Haploid embryonic stem cells

Reporter： XueBinghuaDate： 2011.12.24

Derivation of haploid embryonic stem cells from mouse embryos

Martin Leeb & Anton WutzNature

7 September 2011

Results:

1 、 Deriving haploid mouse ES cells

B6CBAF1

132 activated oocytes

30 blastocysts (22%)

27 ES cell lines(93%)

LIF+2i[SU5402+PD184352(inhibitor of MAPK and GSK3)]

Requirements for deriving aploid mES cells

1 、 Lif+KOSR+DMEM2 、 Lif+FBS+DMEM+Feeder

Results:

2 、 DNA content

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3 、 Morphology and Karyotype

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4 、 Comparative genomic hybridization

g

CBA control, black; HAP-1, red; HAP-2, green

HTG control, black; HTG-1 , green ; HTG-2 , red

Results:

5 、 Pluripotency markers

Results:

6 、 High correlation : Genome-wide

99 X-linked genes were over expressed and 4 Y-linked genes were

lost in haploid ES cells

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7 、 Differentiation potential :Chimaeras

Results:

8 、 Utility : Genetic screening

1、 6-TG（ 2氨基 6巯嘌呤）2、 Spilinkerette PCR