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MODEL HALO DB-20 UV-VIS SPECTROPHOTOMETER
HALO DB-20 Spectrophotometer Instruction Manual
Please read through this manual carefully and store it in a safe place. Before using the instrument, please read the safety
instructions and precautions carefully Keep this manual in a safe place nearby to access
INSTRUCTION MANUAL HALO DB-20
UV-Vis Spectrophotometer
Read and keep this manual · Read the safety instructions carefully and understand them before using the
equipment. · Keep this manual at hand for reference.
Dynamica Ltd.
Version No. V1.1
CONTENTS PREFACE……………...……….………..…….…….………PREFACE-1
IMPORTANT……………...……….………..….…….…IMPORTANT-1
Precautions on Electromagnetic Wave Interference……………………………………………………………………IMPORTANT-1 1. Possible Electromagnetic Wave Interference Caused by This Instrument………………………………………………………..IMPORTANT-1 2. Possible Electromagnetic Wave Interference Affecting This Instrument…………………………………………………………..IMPORTANT-1 Warranty on Product………………………………………………………..………IMPORTANT-2 Technical Service……………………..……………………………………………IMPORTANT-4 Disposal this instrument……………………………………………………..……IMPORTANT-5 Other Precautions…………………………………………………………..………IMPORTANT-5 1. Handling of Chemicals and Samples… ........................................... … IMPORTANT-5 2. Notice on Instruction Manuals ........................................................ … IMPORTANT-5 3. Trademark Acknowledgments….……………………………………IMPORTANT-5
SAFETY SUMMARY……………...……….……….SAFETY-1
THE PERSONAL COMPUTER…………………..……………SAFETY-10
COMPUTER VIRUSES AND
BACKUP DATA………………………….………………………….SAFETY-10
WARNING DISPLAY LABELS…………………………………….SAFETY-11
CHAPTER 1 INSTALLATION………………….……………………….1-1 1.1 UNPACKING…………………………………………………..…………………………1-1
1.2 INSTALLATION SITE………………….……………………………………...………..…1-2
1.2.1 Installation Conditions…………………….………….…………………………….1-2
1.2.2 Installation Environment……………………………….…………………………..1-2
1.3 CHECK OF CONTENTS……………………………...……………………………………1-3
1.4 CHECK OF VOLTAGE AND FUSE……………………………...………………………….1-4
1.5 SIDE PANEL OF THE DB-20………………………..………..………………………1-5
1.6 CONNECTION OF POWER CORD AND GROUNDING WIRE……………...…………………1-5
1.7 CONNECTION OF PRINTER………………………...……………………………………..1-6
1.8 LOCAL-REMOTE SWITCH…………………….………………………………………….1-7
1.9 LCD BRIGHTNESS ADJUSTMENT PORT…………………………………………………1-7
I
1.10 CONNECTING ACCESSORY TO DB-20……………….………………..…………….1-8
1.11 CONNECTING DB-20 TO PC SOFTWARE………………….……..………………1-8
1.12 CONNECTING USB FLASH MEMORY…..……………………………………………….1-8
CHAPTER 2 FUNCTION…………………………………………………2-1
2.1 APPLICATION……………………………………………………………………………2-1
2.2 PRINCIPLE OF OPERATION……………………………………………………………….2-2
2.2.1 Optical System……………………………………………….……………………..2-2
2.2.2 Signal Processing and Control System……………………………………………..2-3
2.3 PROPER USE OF SPECTROPHOTOMETER…………………………………...…………….2-3
2.3.1 Solvent Selection…………………………………………………..………………2-3
2.3.2 Special Samples……………………………………………………………………2-4
2 .4 F U N C T I O N S …………………………………………………………………2-4
2.4.1 Quantitative Analysis……………………………………………..………………2-4
2.4.2 Wavelength Scan…………………………………………………….…………….2-5
2.4.3 Time Scan…………...……………………………………………….…………….2-6
2.4.4 Secondary Processing Functions…………………………………….…………….2-6
2.4.5 Data Storage Function…………..……………………………………………….2-7
2.4.6 Automatic Start Function………………..……………………………………….2-8
2.4.7 Automatic Calibration and Self – Diagnosis Functions……………………….2-8
2.5 SPECIFICATION………………………………………………………………………….2-8
CHAPTER 3 OPERATIONAL METHOD…………………………………3-1
3.1 BASIC OPERATION……………………………………………………………………....3-1
3.1.1 Operational Procedure……………………………………………………………..3-1
3.1.2 Key-Panel Functions………………………………………………………….…3-1
3.2 PRELIMINARY OPERATION………………………………………………………………3-3
3.2.1 Initialization………………………………………………………………………..3-3
3.2.2 Main Menu…………………………………………………………………………3-4
3.3 PHOTOMETRY……………………………………….…………………………………..3-5
3.3.1 Step by Step Operation……………………………...……………………………..3-6
3.3.2 Performing Photometric Measurements………………………..……………….3-12
3.4 WAVELENGTH SCAN…………………………………………………..………….3-20
3.4.1 Measurement Parameter Setting………………………………………………….3-20
3.4.2 Performing a Wavelength Measurement…………...…………………………….3-28
II
3.4.3 Secondary Processing…………………………………………...………………..3-29
3.4.4 Wavelength Scan Sampling Interval List…..…………………...………………..3-33 3.5 TIME SCAN………………………………………...…………………………………..3-34
3.5.1 Measurement Conditioning Setting……………………………..….…………….3-34
3.5.2 Performing a Time Scan Measurement…………………….…………….………3-38
3.5.3 Secondary Processing……………………………………….……………………3-39
3.6 MULTIPLE WL…………………………………………………………………………3-46
3.6.1 Measurement Conditions Setting……………………………………..…………..3-46
3.6.2 Performing Multiple WL Measurement…………………………………….……3-53
3.7 FILE MANAGER………………………………………………………………...………3-55
3.7.1 Inside Memory…………………………………………………………..………..3-55
3.7.2 Flash Memory…………………………………………...………………………..3-56
3.8 CALIBRATING THE WAVELENGTH…………………………………………………...…3-58
3.8.1 Calibrating the Wavelength………………………………………………………3-58
3.8.2 Set Time……………………………………………………………………..……3-59
3.8.3 Recording Lamp Usage…………………………………………………….…….3-60
3.8.4 GLP/GMP…………………………………………………………………...……3-60
3.9 TEST MENU…………………………………………………………………………..3-65
3.9.1 Retrieving a Test Stored in Inside Memory………………………….…..3-65
3.9.2 Deleting Test From Inside Memory………………………………………3-65
3.9.3 Autostart Function………………………………………………………………3-66
CHAPTER 4 MAINTENANCE…………………..………….…………….4-1
4.1 SELF DIAGNOSIS AND AUTOMATIC ADJUSTMENT………………….….………………..4-1
4.2 CONFIRMATION OF SPECIFICATIONS…………………………….….….……..…………4-1
4.2.1 Wavelength Accuracy…………………………………………..….….…………4-1
4.2.2 Spectrum Bandwidth………………………………………….…...……………4-2
4.2.3 Check for Baseline Flatness……………………………………..……………….4-2
4.2.4 Confirmation of Baseline Flatness……………………………..…………………4-3
4.3 PERIODIC INSPECTION………………………………………………..…….………….4-4
4.3.1 Cleaning Sample Compartment……………………………….….………………4-4
4.3.2 Cleaning the Sample Compartment…………………………….….……………..4-6
4.4 TROUBLESHOOTING………………………………………………….….……………..4-7
CHAPTER 5 REPLACEMENT OF PARTS…………………….……….5-1
5.1 CONSUMABLES AND SPARE PARTS ……………………………………………………..5-1
III
5.2 PARTS REPLACEMENT…………………………….………………….………………….5-1
5.2.1 Replacement of Light Source Lamp…………………………...…………………..5-1
5.2.2 Change Source Lamp Usage Time………..….……………………………………5-4
Notice……...………………………. …………….…………………….………. A
IV
PREFACE Thank you very much for your purchase of Dynamica HALO DB-20 Spectrophotometer. This instruction manual has been prepared for users of the DB-20. It describes how to handle the Spectrophotometer. The HALO DB-20 Spectrophotometer is intended for the purpose of photometric analysis. Do not use it for any other purpose. The HALO DB-20 is designed for use by persons having a basic knowledge of chemical analysis. Other persons should operate the instrument in the presence of someone who has such basic knowledge. Remember that improper use of the instrument, chemicals or samples would result not only in wrong analytical data but in consequences adverse to safety. Before using the instrument, be sure to read through this instruction manual to enable correct use. For installation/maintenance of the instrument, read the instruction manual carefully to attain a full understanding of the instructions. Be sure to observe cautionary instructions. Keep this manual handy for easy reference when necessary. Information contained in this manual is subject to change without notice for product improvement.
ABOUT THIS MANUAL
Before using the instrument, be sure to read through this instruction manual. First of all, read “SAFETY SUMMARY” included at the beginning of this manual for ensuring safety in operation of the spectrophotometer main unit.
PREFACE-I
IMPORTANT Precautions on Electromagnetic Wave Interference 1. Possible Electromagnetic Wave Interference Caused by This Instrument
When this instrument is used in a residential area or an adjacent area thereto, it may cause interference to radio and television reception. To prevent this, use the specified system connection cables in strict accordance with the instruction manual. The instrument is designed to minimize possible electromagnetic wave interference caused by it if the specified cables are connected properly. However, there is no guarantee that electromagnetic wave interference will not be caused by the instrument. If the instrument does cause interference to radio or television reception, which can be determined by turning off and on the instrument, the user is encouraged to try to correct the interference by one or more of the following measures: • Increase separation between the instrument and radio/TV
receiver. • Connect the instrument to an outlet on a circuit different from
that to which the radio/TV receiver is connected. 2. Possible Electromagnetic Wave Interference Affecting This Instrument
If this instrument is used near an intense electromagnetic source, interference noise may be given to the instrument to incur an adverse effect on its performance or functionality. To prevent this, use the specified system connection cables in strict accordance with the instruction manual. The instrument is designed to minimize possible electromagnetic wave interference affecting it if the specified cables are connected properly. However, there is no guarantee that electromagnetic wave interference will not occur in this instrument. If the instrument does incur electromagnetic wave interference, which can be determined by turning on and off possible sources of electromagnetic wave interference nearby, the user is encouraged to try to correct the interference by one or more of the following measures:
IMPORTANT-1
• Reorient the instrument. • Increase separation between the instrument and possible
sources of electromagnetic wave interference. • Increase separation between the power cable of the instrument
and possible sources of electromagnetic wave interference. • Connect the instrument to an outlet on a circuit different from
that to which possible sources of electromagnetic wave interference are connected.
• Confirm that any other device connected with the instrument is not affected by electromagnetic wave interference.
Warranty on Product
The Model HALO DB-20 Spectrophotometer is warranted to operate according to the specifications given in the instruction manual, provided it is used in accordance with the instructions described in the manual. (1) Scope of Warranty
(a) Any parts which prove to be defective in design or workmanship during the warranty period will be repaired without charge.
(b) A substitute part may be used for repair, or replacement
with an equivalent product may be made instead of repair. (c) Such system components as a personal computer and
printer to be updated frequently for improvement may not be available in original versions at the time of replacement.
(2) Warranty Period
One year from the date of initial installation. Consumables such as WI and D2 lamps are excluded from warranty.
(3) Availability of Technical Support Service
Technical support service for this instrument is available within regular working hours on workdays predetermined by us.
IMPORTANT-2
(4) Limitations and Exclusions on Warranty Note that this warranty is void in the following cases, even if they occur within the warranty period.
(a) Failure due to operation at a place not meeting the
installation requirements specified by us (b) Failure due to power supply voltage/frequency other than
specified by us or due to abnormality in power supply (c) Corrosion or deterioration of the tubing due to impurities
contained in reagent, gas, air or cooling water supplied by the user
(d) Corrosion of the electric circuits or deterioration of the
optical elements due to highly corrosive atmospheric gas (e) Failure due to use of hardware, software or spare parts
other than supplied by us (f) Failure due to improper handling or maintenance by the
user (g) Failure due to maintenance or repair by a service agent
not approved or authorized by us (h) After disposal of this instrument, or after its resale without
our approval (i) Failure due to relocation or transport after initial
installation (j) Failure due to disassembly, modification or relocation not
approved by us (k) Consumables, and failure of parts that have reached the
end of specified useful life (l) Failure of parts excluded from the warranty in the
instruction manual or other documents (m) Failure due to acts of God, including fire, earthquake,
storm, lightning, social disturbance, riot, crime, insurrection, war (declared or undeclared), radioactive pollution, contamination with harmful substances, etc.
IMPORTANT-3
(n) Failure of the hardware, or damage to the system software, application software, data or hard disk due to computer virus infection
(o) Failure of the personal computer connected with the
instrument, or damage to the system software, application software, data or hard disk due to power interruption or momentary power voltage drop caused by lightning or the like
(p) Failure of the personal computer connected with the
instrument, or damage to the system software, application software, data or hard disk due to disconnection of main power to the personal computer without taking the specified normal shutdown procedure
(5) Disclaimer of Warranty
(a) Any express warranties other than the explicit conditions
indicated in (1) are excluded from this warranty. Any other implied warranties of merchantability and fitness for a particular purpose are not included in this warranty. No liability is assumed for direct or indirect damages arising out of explicit or implied warranties.
(b) Oral or written information or advice given by our dealers,
distributors, agents or employees without our express permission shall not create a warranty or in any way increase the scope of this warranty.
Technical Service Installation of this instrument shall be carried out by or under supervision of qualified service personnel of Dynamica or its authorized service agent. Before installation of the instrument, the user shall make preparations for satisfying the installation requirements in accordance with the instruction manual. If relocation of the instrument becomes necessary after initial installation (delivery), please notify your local sales representative or nearest service office of Dynamica.
IMPORTANT-4
Disposal this instrument
When you discard equipment, please check and discard a related statute etc. or ask the service section of Dynamica.
Other Precautions 1. Handling of Chemicals and Samples
(1) The user is responsible for following relevant legal standards
and regulations in handling, storage and discarding of chemicals and samples used in analytical operations of this instrument.
(2) Reagents, standard solutions and accuracy-control samples
shall be handled, stored and discarded as instructed by the respective suppliers.
2. Notice on Instruction Manuals
(1) Information contained in the instruction manuals furnished
with the instrument is subjected to change without notice for product improvement.
(2) This manual is copyrighted by Dynamica with all rights
reserved. (3) No part of this manual may be reproduced or transmitted in
any form or by any means without our express written permission.
3. Trademark Acknowledgments
Microsoft, Windows, Microsoft Excel, Microsoft Word and Windows XP are registered trademarks, trademarks or trade names of Microsoft Corporation, USA, while other company names and product names are those of respective companies.
IMPORTANT-5
SAFETY SUMMARY
For your safety please read the following precautions carefully before using the DB-20 UV-Vis Spectrophotometer.
General Safety Guidelines For safe handling of this product, please follow the instruction
procedure in the manual for this product. Pay special attention to follow all the hazard warnings on the product
and in the manual. Failure to do so can cause injury to you or damage to the product.
After installation, please do not move the equipment. A vibration might
affect the adjustment of the product. The hazard warnings, which appear on the warning labels on the
product or in the manual, have one of the following alert headings consisting of an alert symbol and a signal word, DANGER, WARNING, or CAUTION.
DANGER: Indicates an imminently hazardous situation that, if notavoided, will result in death or serious injury.
(It does not apply to this equipment.)
WARNING: Indicates a potentially hazardous situation that, if notavoided, can result in death or series injury.
CAUTION: Indicates a hazardous situation that, if not avoided, will or can result in minor to moderate injury, or serious damage to the product.
: The alert symbol shown precedes every signal word forhazard warnings, and appears in safety relateddescriptions in the manual.
SAFETY-1
SAFETY SUMMARY (Continued)
General Safety Guidelines (Continued)
In addition, the following “Attention” and “Note” are not directly related to the safety of a person:
SAFETY-2
Attention: It is used to present warnings, which are notdirectly related to personal injury hazards. It is used to indicate prevention against damage to theequipment.
Note: This is used to indicate instructions that enable you
to operate the equipment accurately and performaccurate measurements.
SAFETY SUMMARY (Continued)
General Safety Guidelines (Continued) Before Using
Before using this product, please make sure you read and understand the instructions.
Please keep this manual in a safe and easily accessible place so that you can use it
when necessary.
Please make sure to use this product properly and follow the instructions as specified in this manual.
Please make sure to understand and follow the instructions regarding safety in this
manual.
If you do not follow the instructions in this manual, an inaccurate analysis may result or bodily injury may occur.
Because of danger, please make sure not to modify or alter the product, make sure
not to use unspecified parts, and make sure not to operate the equipment by removing/defeating the safety device(s).
When using chemicals, please make sure to ventilate the room well. If there is not
enough ventilation, it may be hazardous to your health.
Although we have carefully considered the instructions written on the products and manuals, it is possible for an unexpected event to occur. When operating the equipment, aside from following the instructions, be very cautious.
SAFETY-3
SAFETY SUMMARY (Continued)
General Safety Guidelines (Continued) Precautions for Installation • Maintenance • Relocation and After Sale Technical Service
Before installation, confirm that there are no missing items or standard accessories. If there is something missing or damaged, or you have noticed any problems, please contact our nearest representative.
Operating the equipment without a standard part can damage the
equipment and cause safety concerns. If that occurs, please follow the instruction of the installer.
Installation of this instrument shall be carried out by or under
supervisions of qualified service personnel of Dynamica or its authorized service agent.
When relocation of this instrument becomes necessary after initial
installation (delivery), please notify your local Dynamica sales representative or nearest Dynamica service office. Technical support service for this instrument is available from s service agent approved or authorized by Dynamica within regular working hours or workdays.
Please do not perform any other operations that are not included in
the manual. If any problem occurs with the equipment, please contact the agent from whom you purchased it or the service department of Dynamica.
SAFETY-4
SAFETY SUMMARY (Continued)
WARNING: Poisoning from Organic Solvent Gas Handling Organic Solvents
The organic solvent vapor may be harmful to your health.
WARNING: Eye Injury from Organic Solvents Handling Organic Solvents
Please wear protective glasses when using organic solvents. If the organic solvent should get into your eye, flush your eye immediately under running water for at least 5 minutes while keeping your eyelids open. See a physician for appropriate treatment.
SAFETY-5
SAFETY SUMMARY (Continued)
WARNING: Electrical Shocks from Improper Grounding Electrical Shock from Improper Grounding
When wiring the personal computer, power supply for the thermostatic cell holder and the like, please make sure to use the 3-prong wire (with ground) provided.
WARNING: Electrical Shock Electrical Shock from Contact
High voltage is used inside of the equipment. Never remove the cover except by a service person or as instructed in the section for lamp replacement.
When cleaning the inside of the cell compartment, be sure to turn off the
unit.
CAUTION: High Temperature Burns from high temperatures
The lamp will become very hot during operation, please make sure that the D2 lamp and the tungsten lamp is cooled off when replacing the lamp.
SAFETY-6
SAFETY SUMMARY (Continued)
CAUTION: Injury from incidental contact with the unit.
Handling the sample compartment cover
When you open and close the sample compartment cover, please be careful not to get your hand or hair caught. Please assure your safety before operation.
CAUTION: Fatigue due to Prolonged Work Data Processing
Viewing the display in your work can cause eye and physical fatigue if you continue to work in the same posture for extended periods.
When working with the display for a prolonged period, for your health, make sure to take breaks for 10 to 15 minutes every hour in order to rest your eyes and body.
SAFETY-7
SAFETY SUMMARY (Continued) NOTE Precautions regarding Accuracy and Precision of Measurements
Do a routine check prior to taking measurements. In order to obtain accurate measuring results, please perform tests as instructed, following the instructions in Chapter 3.
SAFETY-8
SAFETY SUMMARY (Continued) Electricity
Voltage supplied to the DB-20 UV-Vis Spectrophotometer. The voltage for the Spectrophotometer system and personal computer must be a single-phase AC 100V to 200V; Variations in the voltage and noise generated in the power line will cause adverse effects on the UV-Vis spectrophotometer and may also cause accidents.
Please make sure that grounding is provided together with the power
supply wires, and make sure that it is connected with a grounding resistance of less than 10 Ω. Defective grounding may not only cause lower resistance against noise from the outside but it can also cause the Mass Spectrometer and personal computer to generate static electricity, which may involve the danger of electrical shocks.
A high voltage circuit is used inside the UV-Vis Spectrophotometer. Do
not open the covers when this circuit is operational because of the danger from electrical shock.
Fire Extinguishers
Do not smoke or use fire within 3 meters of the UV-Vis Spectrophotometer
Make sure to keep a fire extinguisher near the UV-Vis Spectrophotometer
at all times. Obtain an ABC Powder extinguisher that can be used for normal fires, oil fires, and electrical fires.
SAFETY-9
SAFETY SUMMARY (Continued) The Personal Computer
When turning off the power to the personal computer while the hard disk, floppy disk, or MO drive (optional) is still running, the computer may malfunction or the data and software can be destroyed.
Computer Viruses and Backup Data
If any program/data is damaged suddenly or an unexpected operation/screen is encountered, the personal computer may be infected by a computer virus. Computer viruses are malicious programs that sneak into the personal computer to cause misbehavior or damage to data. A program designed to offer protection against computer viruses is called a vaccine program (virus scan). Do not use any programs or storage media that are suspected of containing a virus. If there is a possibility of virus infection, check for a virus using a vaccine program. It is very important that you make a backup of your hard disk just in case.
SAFETY-10
SAFETY-11
SAFETY SUMMARY (Continued) Warning Display Labels
The following warning label is applied to the mainframe of the DB-20 Spectrophotometer:
High temperature can cause burns. Light source lamp and lamp chamber remain hot for a while even after Power-off. Before lamp replacement, turn power off and wait at least 20 minutes.
134-1973
High voltage: 500V Can cause fatal or serious injury. Before removing light source cover, turn power switch off, Never release safety mechanism inadvertently.
1-1
Chapter 1 Installation
1.1 Unpacking
After unpacking the shipping crate, carefully take out the spectrophotometer and place it on a table. Since the spectrophotometer is as heavy as about 29 kg, special attention should be paid when taking it out.
Fig. 1-1 HALO DB-20 Spectrophotometer
Note: The light source-switching mirror is secured with the sponge rubber material for transport at shipment from the factory. After using, open the light source cover and remove the protective sponge from the light source-switching mirror. The shipping crate cannot be claimed for compensation even if it is damaged during the transportation.
1-2
1.2 Installation site
1.2.1 Installation conditions
Power requirements: Power supply voltage: AC100, 115, 220, 230 or 240V (There must be the
devices for switching voltage.) Allowable fluctuation is within ±10% of rated voltage. Frequency: 50 or 60 Hz
Allowable fluctuation is within ±4% of rated frequency. Power capacity: 200VA or more.
Prepare a capacity more than 500VA in reference with a co-use with other accessory devices.
Note: If warning labels are not readable because of stains on coming off, please notify local Dynamica sales representative or service office. New labels will be provided.
Grounding line: Grounding line resistance of 100Ω or less is required.
Place for Installation Minimum floor area: Floor area with a width of 550 mm and a depth of 710
mm or more is required. Floor strength: Floor strength 30kg or more.
Select a rigid and horizontal plane capable of withstanding the above load as an installation area.
1.2.2 Installation Environment
Operating temperature: 5~35℃. In order to perform a measurement under the most stable condition, we recommend that the instrument is used in an air conditioning room of 20℃ ~ 25℃. Operating humidity: 45% ~ 85%.
1-3
Storage temperature: -20℃ ~ 60℃.
Atmosphere, gas:
Free from acid gas or alkaline gas and other gases that may corrode metals significantly. Free from gases that may dissolve paint such as from organic solvents (particularly benzene, thinner or the like).
Other general notes:
Avoid direct sunlight (otherwise optical performance might deteriorate or housing might be discolored). Avoid a window as much as possible. A strong vibration or shock that can be felt by the human body must not
be transmitted to the instrument (otherwise the fine adjustment mechanism might malfunction). Avoid the vicinity of a heat generating apparatus such as a gas burner,
electric heater or oven in order to prevent the mainframe cover being heated (beyond 70℃). Avoid the vicinity of instruments that generate a strong electric field
(such as an electric welding machine, high frequency furnace or pole transformer). Avoid a dusty environment (otherwise optical performance might
deteriorate). The line voltage must be stable and free from a rapid fluctuation
(otherwise noise might increase). Do not turn on and off frequently electric instruments (stirrer, vibrator,
etc) that are not provide with a noise-preventive device in the same power line as for the mainframe.
Note: The optical system is very delicate. And the control unit incorporates high-density electronic circuit components that function as a computer. So the above cautions should be observed strictly.
1.3 Check of Contents
After unpacking, check the contents of delivery against the packing list. In the DB-20, you should find the following items.
1-4
DB-20 Main unit Operation Manual Power cord with fuse Standard cuvette holder for DB-20 Dust cover One Philips and one minus screw drivers
If any part is missing or damaged, or if you have a question, contact the nearest Dynamica sales representative.
Fig. 1-2 Standard Accessories
Note: The standard accessories are subject to change for improvement withoutprevious notice. Check them for proper quantity against the packing list furnished with the instrument.
1.4 Check of Voltage and Fuse
Please first check if the line voltage is suitable for the line voltage allowed using labeled beside the power connector.
Warning: Before proceeding to the work, make sure the power cord is not connected to the wall socket.
Note: There is the voltage selector switch to select the line voltage allowed to
use. Confirmation of the Fuse
1-5
Select the fuse in accordance with the line voltage to be used.
Voltage Fuse Capacity 100 V 3.15A 220 V 3.15A
Fig. 1-3 Power Connector of HALO DB-20
1.5 Side Panel of the DB-20
The DB-20 has 6 ports on the side panel. They are: Power connector and switch Printer port LCD brightness adjustment port Local-Remote switch for PC software control or Local keypad control Acc. Port for connection of accessories such as auto sipper. 9- pin Serial port for connection to personal computer
1.6 Connection of Power Cord and Grounding Wire
Note: Before plugging in the power cord, be sure to turn off the POWER switch and be sure to select proper power cord. The capacity of the power cord should be over 250V and 6A.
1-6
Plug the power cord securely into the connector of the mainframe. When using a plug adapter of table tap, be sure its grounding wire to the grounding terminal securely.
Fig. 1-4 How to Ground the HALO DB-20
1.7 Connection of Printer
Connect the printer with the instrument if to use the printer with a standard parallel printer cable. The connector of printer is on the right side of the
1-7
instrument as show in Fig 1-3. DB-20 is compatible with Seiko thermal printer, Hewlett Packard printer with PCL3 setting, Epson Printer with ESC/P setting, Seiko Printer with DPU setting. For a detail list of the printer that is compatible with the DB-20, contact your local representative of Dynamica. To define the printer type connected to the DB-20, please refer to the System setting section interface under printer setting sub menu. Please refer to the relevant instruction manual of printer to install the printer.
1.8 Local-Remote switch
DB-20 can be controlled by the optional PC software, or simply via keypad control. Please put the Local-Remote switch to Local when using keypad and LCD on the DB-20. When connecting to the PC software, switch the Local-Remote switch to Remote position. The LCD will display < PC Control > when connected to PC software for complete instrumentation control and data analysis.
1.9 LCD Brightness adjustment port
Using a small minus screwdriver, the LCD brightness can be adjusted through this port by turning the screw.
LCD brightness adjustment port
1-8
1.10 Connecting Accessory to DB-20
The Acc port is for the connection and control of a variety of optional accessories available from Dynamica such as Auto Sipper, Electronic Thermostatic cell holder. Connect the optional accessories using the cable provided along with the optional accessories. Please refer to the installation manual for detail for installation of optional accessories.
1.11 Connecting DB-20 to PC Software
Optional PC software allows DB-20 to be fully controlled by the software and perform data analysis using the PC software. Connect the serial cable provided with PC software to the port marked PC. Please also set the Local-Remote switch to Remote when using PC software.
1.12 Connecting USB Flash Memory
DB-20 is equipped with a USB port to connect commercial available USB flash memory for the storage of data generated by the DB-20. The data generated in the DB-20 can save into USB flash memory with File Manager of DB-20. Refer to the relevant chapter for operation. The data can be transferred to standard computer for storage also. Simply insert the USB flash memory disk into the port. USB flash memory of Kinston Data Traveller ( Compatible to USB1.1 and FAT file system ) is tested to be compatible. Contact your Dynamica sales agent to obtain the latest list of the compatible Flash Memory.
1-9
2-1
Chapter 2 Function
2.1 Application
The HALO DB-20 Spectrophotometer is designed for absorption analyses of liquid, solid and gaseous samples in ultraviolet and visible spectral regions. In this scheme (Fig.2-1), a monochromatic beam with intensity ‘I0’ travels through a liquid phase having concentration ‘c’ and path length ‘l’ , which results in the intensity of monochromatic radiation decreasing to ‘It’. With respect to the initial intensity ‘I0’ and attenuated intensity ‘It’ of monochromatic radiation, the following equation (1) can be set up. It/I0 = 10-ε·c·l =τ……………………………………………………….(1) Where, ‘ε’ is a constant known as absorptivity, which varies depending on the sample. Equation (1) represents the statement of Lambert – Beer law, and ‘τ’ indicates transmittance which is often expressed as a percentage, i.e. ‘τ× 100 = T’ (percent transmittance or %T). Also, the common logarithm of inverse transmittance can be expressed as follows: Log (l/τ) = ε·c·l = E …………………………………………………..(2) Where ‘E’ is called absorbance (abbreviated as Abs). Absorbance ‘E’ is proportional to concentration ‘c’ and is a unit of measurement indispensable for quantitative determination.
Fig.2-1 Spectrophotometry Principle Scheme
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2.2 Principle of Operation
2.2.1 Optical System
Figure 2-2 shows the optical system of the HALO DB-20.
Sample Compartment Monochromator
Detector 1
Lens Sample Beam
Grating
Enter Slit Mirror （M1）
WI lamp
Ｄ２ lamp
Filter (F)
Exit Slit
Half Mirror （HM）
Mirror（M3）
Mirror（M4）
Detector 2
Mirror（M2）
Reference BeamLens
Fig 2-2 Optical System of HALO DB-20 Spectrophotometer
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DB-20 is a double beam system, which features a half mirror to split the beam to reference beam and sample beam. The white light emitted from the light source is fed to the Seya-Namioka monochromator utilizing a concave diffraction grating (with a grating constant of 1/600 mm, a blaze wavelength of 250nm and a grating area of 20mm × 25mm), where it is transformed into a monochromatic beam. The beam sent from the monochromator is reflected by the toroidal mirror (M2), and then separated into reference beam and sample beam by the half mirror (HM). The two beams have passed through the sample compartment are focused by lenses, and are then irradiated into the detector (D1 and D2) where they are converted into electric signals. Listed below are the features of this optical system. The Seya-Namioka optical system utilizing Dynamica’s reputable concave diffraction grating realizes a monochromator featuring high-energy efficiency and low stray light level. The beam is separated by using a half mirror rather than a rotary mirror like in the conventional double beam spectrophotometer. This structure serves to eliminate the moving parts, thus realizing a highly stable and reliable monochromator. The optical systems on the sample and reference beam sides are absolutely the same to enable acquiring highly stable data.
2.2.2 Signal Processing and Control System
The electric signal converted from optical signal enters the LOG amplifier where it is LOG converted to provide absorbance data. This data is amplified, and is then A/D converted to be processed as digital value. The processed results are displayed on the LCD and recorded onto the printer.
2.3 Proper Use of Spectrophotometer
2.3.1 Solvent Selection
When selecting a solvent for sample preparation, keep the following requirements in mind. Small absorption in the spectral region of measurement wavelength. Non – interactive with solute.
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Low volatility. Table 2-1 lists the applicable wavelength ranges of common organic solvents.
Table 2-1 Wavelength Ranges for Common Solvents Solvent Wavelength Range Cyclohexane 200nm ~ 400+ nm Ethyl alcohol 220 nm ~ 400+ nm Methyl alcohol 220 nm ~ 400+ nm Ethyl ether 220 nm ~ 400+ nm Dioxane 220 nm ~ 400+ nm Hexane 220 nm ~ 400+ nm Chloroform 250 nm ~ 400+ nm Isopropyl alcohol 250 nm ~ 400+ nm Acetic acid 250 nm ~ 400+ nm Ethyl acetate 270 nm ~ 400+ nm Carbon tetrachloride 275 nm ~ 400+ nm Benzene 280 nm ~ 400+ nm Methyl ethyl ketone 335 nm ~ 400+ nm Acetone 340 nm ~ 400+ nm Carbon disulfide 380 nm ~ 400+ nm
2.3.2 Special Samples
Note that the laws expressed by Equations (1) and (2) are not applicable to the following special samples. Fluorescing sample Appreciably turbid sample In measurement of a solid sample as glass plate, the energy of beam radiation undergoes a loss (r) due to reflection on the surface of solid substance. In this case, Equation (1) must be transformed as shown below. It /I0 = 10-ε.c.l – r ………………………………………………. …(3) Where, ‘r’ varies depending on the reflectance of substance.
2.4 Functions
2.4.1 Quantitative Analysis
The HALO DB-20 is capable of calculating the concentration of a sample after generating a standard calibration curve or by entering a factor into the system. The following are the features of this function. (1) Generation of standard calibration Curve
(a) Up to twenty samples can be measured, and the acquired values are connected to each other with segmented lines or regression lines determined by the least squares method for generating a working curve.
(b) Specific standard samples can be measured again to regenerate a working curve.
(c) A working curve can be regenerated upon deleting specific standard samples.
(d) Working curves can be stored as a part of measuring conditions. It is possible to store the curves for a maximum of twenty tests.
(e) Working curve can be graphically displayed on the LCD, and a value at a desired point can be read by moving the cursor to that point.
(f) Analytical results are displayed on the LCD together with the working curve. Thus, the results can be confirmed while observing the working curve.
(g) Working curve can be graphically plotted on the printer. (h) Working curve can be generated by entering factor values. (i) It is possible to generate a working curve by 2 - /3 – wavelength
measurement.
(2) Statistical Calculation This function enables the instrument to acquire mean value, standard deviation (SD) and coefficient of variation (CV).
(3) The upper and lower limit values of analytical data can be judged.
2.4.2 Wavelength Scan
(1) The highest wavelength scan speed is 3600nm/min. Wavelength ranging from 1100~190nm can be scanned in a little over 20 seconds even if the filter and light source are switched over halfway.
(2) Wavelengths can be scanned repeatedly for recording spectra of a sample
that changes with time. (3) Photometric values can be printed out at the specified wavelength interval.
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(4) Photometric values can also be displayed on the LCD. (5) Graphic display on the LCD can be output onto the printer. It takes a little
over 10 seconds to make a hard copy for the graphic display on the LCD.
2.4.3 Time Scan
(1) A graph can be displayed with time graduated on the abscissa. (2) Photometric values can be printed out at the specified time interval. (3) Data can be calculated for right assay.
2.4.4 Secondary Processing Functions
The HALO DB-20 can store in the memory the measured spectrum and its change with time. Secondary processing can be applied variously to the stored data. Automatic Data Readout
A cursor can be moved to a desired point in the spectrum currently displayed on the LCD screen and a reading of the absorbance (transmittance) at that point.
Peak Detection
Cursor can be moved to a peak position to display absorbance (transmittance) readings at the peak. In addition, an automatic peak selection routine allows peak and valley measurements to be printed in list format on the printer.
Scale Expansion
Three methods are available for expanding the graphic display on the LCD.
(a) Simultaneous Expansion of X and Y Axes Cursor should be moved for pointing out a desired expansion range.
(b) Expansion of X Axis Numeric values should be input to specify a desired expansion range for the abscissa.
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(c) Expansion of Y Axis
Numeric values should be input to specify a desired expansion range for the ordinate.
Smoothing
A spectrum with a large noise level can be corrected to form a smooth curve. Differentiation
The data of a spectrum currently displayed on the LCD are differentiated. The derivative order applicable ranges 1.
Integration
A peak area can be determined by specifying the two wavelengths or times between which you wish the integration calculated. The DB-20 then integrates the area under the spectral curve between the specified points and points out the results.
Calculations Between Spectra
You can calculate addition, subtraction, multiplication or division between two spectra or calculate a constant value with the resulting data displayed on the LCD.
Recalculation During Rate Assay (Kinetics)
Upon completion of a rate assay in the Time Scan mode, you can adjust the calculation start time and end time to allow recalculation of the data.
2.4.5 Data Storage Function
The following data are stored in the memory, and are not deleted even if the instrument power is turned off. Analytical conditions for 20 tests
The working curve prepared for each test can also be stored together with the analytical conditions.
Up to 10 measurement results in inside memory. You also can store the measurement results in flash memory which can be open via personal computer.
Three baselines (System baseline and two user-defined baseline.)
Instrument parameters that were used most recently.
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2.4.6 Automatic Start Function
When the automatic start function is set in advance, the operator need only turn on the power switch (measuring conditions are set automatically), set a sample in the instrument and then press the START key. The rest is done automatically.
2.4.7 Automatic Calibration and Self – Diagnosis Functions
a) Memory (RAM and EPROM) check b) Wavelength drive mechanism check c) Lamp ignition check d) Automatic wavelength calibration
Wavelength is automatically calibrated with reference to that of the beam emitted from the D2 lamp.
e) Baseline Correction
2.5 Specifications
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Table 2-1 DB-20 Specifications Item Description Monochromator Equipped with high resolution
concave diffraction grating and Seya-Namioka monochromator
Wavelength Range 190~1100nm Spectral Bandwidth 1.5 nm Stray Light 0.05% or less (220nm NaI, 340nm
NaNO2) Wavelength Accuracy ±0.3nm Wavelength Reproducibility 0.1nm Photometric Mode Abs, %T, Conc
Photometric Range Abs: -3.000~3.000 %T: 0~600%T Conc: 0.000~9999
Photometry Accuracy (Measured with NIST 930D Filter)
±0.002Abs (0~0.5Abs) ±0.004Abs (0.5~1.0Abs) ±0.008Abs (1.0~2.0Abs) ±0.3%T
Photometry Reproducibility (Measured with NIST 930D)
±0.001Abs (0~0.5Abs) ±0.002Abs (0.5~1.0Abs) ±0.004Abs (1.0~2.0Abs) ±0.15%T
Baseline Flatness ±0.002Abs ( 200 to 950 nm) Baseline Stability 0.0003Abs/h (at 500 nm)
2 hours after power up Noise Level 0.0003Abs/300s (500nm) Wavelength Scan Speed 10, 100, 200, 400,
800,1200,2400,3600nm/min Light Sources WI lamp, D2 lamp Light Source Switchover Automatic changeover, 325-370nm
selectable Detector Silicon photodiode Display Liquid Crystal Display (LCD) Printer Interface PCL/3,ESC/P or DPU Expanded Storage USB Disk Communication Function RS-232C Dimensions 500(W) ×705 (D) ×225 (H) Weight Approx. 29kg Power Source 100, 115, 220, 230, 240V 50/60Hz Power Consumption 200VA
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Chapter 3 Operational method
3.1 Basic Operation
3.1.1 Operational Procedure
The user interface of the HALO DB-20 is convenient and easy-to-use. Screen prompts help you to select the variables that are required for method set-up and data acquisition.
3.1.2 Key-Panel Functions
The illustration below shows the operation panel of the HALO DB-20. There three groups to touch keys on the panel: Special function Numerical Control
Key Functions The function of each key on the HALO DB-20 key panel is described below:
Main Menu List the six data processing functions and retrieves the initial main menu frame. Clear Return This key acts as a backspace key to erase the last character entered or acts as a return key to the previous screen display. Left Arrow Moves the trace left or the page up.
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Right Arrow Moves the trace right or the page down. Up Arrow Moves the highlight or the page up. Down Arrow Moves the highlight or the page down. Enter Enter numeric values specified through the keyboard or selects a test for which analytical conditions is to be set. Goto WL Used to select a desired wavelength. Auto Zero Automatic zero adjustment. In subsequent measurements, each photometric value is determined with reference to the value stored above. Start/Stop Start or stop the measurement. Print Data Used to copy data or spectrum from the screen to the printer. Shift Upper When hold down, this key allows you to use the keyboard keys to enter the upper alphabetical characters. Shift Down When hold down, this key allows you to use the keyboard keys to enter the down alphabetical characters. 0%T To measure the dark current.
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3.2 Preliminary Operation
3.2.1 Initialization
Upon turning on the power switch, the INITIALIZATION frame appears on the display and the instrument performs a self-diagnosis and automatic wavelength calibration. During self-diagnosis your HALO DB-20 spectrophotometer checks the following items.
RAM ROM Wavelength Driver D2 lamp WI lamp AD Conversion 656.1nm
As each item is checked, the message “OK” will display if the item’s function is normal or the message “NG” will display if the item’s function is abnormal.
CONSULT THE TROUBLESHOOTING SECTION IF YOU FIND ERROR MESSAGE DURING INITIALIZATION. Error Message: 1. If the wavelength driver is NG, the system will freeze. And you must power
off the instrument to solve the problem. 2. If the other items are error, the instrument can go on run by pressing
Clear/Return. Or else the system will stop.
Initilization…
RAM OKROM OKWavelength Driver OKD2 lamp OKWI lamp OKAD Conversion OK656.1nm OK
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3.2.2 Main Menu
After initialization, the LCD will enter into the interface which last interface before power off. Press Main Manu key and the Main Menu screen appears on the display as shown in the below. The Main Menu lists seven options plus Wavelength and photometric value displayed on the top of the screen. To guide you through each procedure, there is a prompt displayed at the bottom of each screen page. The function of each option item refers to chapter 2.
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Main Menu
1 Photometry
2 Wavelength Scan
3 Time Scan
4 Multiple WL
5 File Manager
6 Calibration
7 Test Menu
Select Function: _
Selecting Options from the Main Menu You can access a desired option in one of two ways: 1. The numeric key as the function key, just press the number, such as Press
<1> to start the Photometry sub menu. 2. Press Main Menu key to return the Main menu.
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3.3 Photometry
Press [1] on the keypad in the main menu to enter the photometry mode. The photometry submenu will be displayed on the LCD.
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Photometry
1 Parameter Setup
2 Sample Setup
3 Curve Setup
4 Print Setup
5 System Setup
6 Save Parameter0 Forward
Select Function: _
To help you understand the variables for each sub menu option, the general purpose/function of each option is outlined in Table 3-1.
Table 3-1 Photometry Options and Variables Sub menu
Option Variables Range Description of Function
Parameter Setup
Data Mode WL
T%, ABS, Conc 190 ~ 1100nm
Measures transmittance, absorbance and concentration. Wavelength for analysis
Sample Setup
Name 02/11/02 Path Length Unit Label
1-9,A-Z,*,-,space,:,/, Not more than 8 character 10mm Not more than 6 character
Sample title. Specify the concentration units
Curve Setup
Curve Type Curve Data Num STDS
1st order 2nd order Segment K-factor 0.000 ~ 9999 2 ~ 20
Linear-squares fit (y=k1x+k0) Quadratic fit (y=k2x2+ k1x+k0) Straight lines interconnecting curve points Concentration of each standard Number of standards used for fitting the selected curve type
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Print Setup Printer Type ESC/P, DPU,PCL3 System Setup
Lamp change mode Lamp change WL (nm) D2 lamp WI lamp
Auto D2 Lamp Only WI Lamp Only 325.0~370.0 (±0.1nm) On / Off On / Off
Changeover is automatically made between D2 Lamp and WI Lamp Using D2 lamp /WI lamp over the entire wavelength region Changeover wavelength between D2 Lamp and WI lamp Turn on or off the D2 Lamp Turn on or off the WI lamp
When the sample is volatile liquid, pleas must cover the sample cell with lid. Don’t measure the combustible liquid or gas with this instrument. If the sample is spilled over from the sample cell, please blot up immediately with proper paper or cloth lest eroding the instrument.
Cautions:
3.3.1 Step by Step Operation
3.3.1.1 Parameter Setup Press <1> to select Parameter Setup from the photometry submenu, the parameter submenu list appears on the right side of the screen as shown in the illustration below:
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Photometry
1 Parameter Setup 1 Data Mode ABS
2 Sample Setup 2 WL (nm) 500.0
3 Curve Setup
4 Print Setup
5 System Setup
6 Save Parameter
0 Forward
Select Function: _1 T% 2 ABS 3 Conc
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Press the corresponding key on the keyboard to select the function. For example, press <1> to select Data Mode; press <2> to input the wavelength for analysis. Data Mode: The following three data mode is acceptable. %T: (percent transmittance measurement) ABS: (Absorbance measurement) Conc: (Concentration measurement) WL (nm): Determine a wavelength to be used for measurement. This
parameter can be established within a range of 190 ~ 1100 nm in increments of 0.1 nm.
Note: There are always prompts on the bottom of the screen. You can operateaccording the prompts.
3.3.1.2 Sample Setup
Press <2> to select Parameter Setup from the photometry submenu, a submenu list appears on the right side of the screen as shown in the illustration below:
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Photometry
1 Parameter Setup 1 Name : SAMP-1
2 Sample Setup 2 Path Length ( nm ): 10.0
3 Curve Setup 3 Unit Label : MG/L
4 Print Setup
5 System Setup
6 Save Parameter
0 Forward
Select Function: _
Press <1> key to input the sample name. The name cannot be more than eight characters. Press <2> to input the Path Length value, which means: when measurement was preformed using a cell other than 10 mm rectangular cell, the photometric value can be converted into one measured with a 10 mm rectangular cell.
3.3.1.3 Curve Setup
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Press <3> to select Curve Setup from the photometry submenu, a submenu list appears on the right side of the screen as shown in the illustration below:
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Photometry
1 Parameter Setup 1 1st order
2 Sample Setup 2 STD NUM
3 Curve Setup 3 Curve Data 10
4 Print Setup 4 Repeat NUM 1
5 System Setup 5 HI Limit 9999
6 Save Parameter 6 LO Limit 0
7 Forward
Select Function: _
Curve Type
Note: Function 2 to function 6 can only work at the “Conc” data mode.
Curve Type: Press <1> on the keyboard. The following four types of curves selectable are shown in the illustration below.
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Photometry
1 Parameter Setup 1 1st order
2 Sample Setup 2 STD NUM
3 Curve Setup 3 Curve Data 10
4 Print Setup 4 Repeat NUM 1
5 System Setup 5 HI Limit 9999
6 Save Parameter 6 LO Limit 0
7 Forward
Select Function: _
Curve Type
1 1st order 2 2nd order 3 Segement 4 K-factor
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Press corresponding key on the keyboard to select curve type. In which 1st order means linear; 2nd order means quadratic regression curve; segment means polygonal line; K-factor means k-factor input line. If select “1st order”, the standards number should be more than 2. If select “Second order”, the standards number should be more than 3. If select “K-factor”, then you can input the value of k0, k1 and k2.
Note: If the standards number is not fit the curve type, the system will automatically changed the numbers to fit the curve type.
Curve Data: Input the standards concentration.
Press <3> key on the keypad to input the Curve Data. The data list will appear on the right side of the screen as follows. Left and right arrow key are used to select the ID of Standards (STDs).
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Photometry
1 Parameter Setup STD Conc
2 Sample Setup 1 10
3 Curve Setup 2 20
4 Print Setup 3 30
5 System Setup
6 Save Parameter
0 Forward
Input Value:_
(0 to 9999)
STD NUM: (Number of standards) Select the numbers of standards sample. This parameter is
specifiable in range of 2 to 20.
Repeat Num: Indicates the times to repeat to measure the standard sample. Input this value from 1-5.
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HI Limit: The highest value limitation of concentration. If the
concentration more than this value, “HI” will be displayed. The value of concentration is between 0 to 9999.
LO Limit:
The lowest value limitation of concentration. If the concentration less than this value, “LO” will be displayed .The value of concentration is between 0 to 9999.
3.3.1.4 Print Setup
Press <4> to select Print Setup from the photometry submenu, a submenu list appears on the right side of the screen as shown in the illustration below:
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Photometry
1 Parameter Setup 1 ESC/P
2 Sample Setup
3 Curve Setup
4 Print Setup
5 System Setup
6 Save Parameter
0 Forward
Select Function: _
Printer Type
1 ESC/P 2 DPU 3 PCL3
Press the number key on the keyboard to select corresponding function. DB-20 support three types printer, namely EPSON series printer, HP type series printer and Seiko thermal printer.
3.3.1.5 System Setup
Press <5> to select System Setup from the photometry submenu, a submenu list appears on the right side of the screen as shown in the illustration below:
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Photometry
1 Parameter Setup 1 Lamp Change Mode Auto
2 Sample Setup 2 Lamp Change WL ( nm ) 350.0
3 Curve Setup 3 WI Lamp On
4 Print Setup 4 D2 Lamp Off
5 System Setup
6 Save Parameter
0 Forward
Select Function: _
Lamp Change Mode:
The followings three Lamp Change Mode are available. Auto: Changeover is automatically made between D2 Lamp and WI Lamp. D2 Lamp Only: Using D2 lamp over the entire wavelength region. WI Lamp Only: Using WI lamp over the entire wavelength region.
Lamp Change WL (nm):
This parameter is used for selection of light source switching wavelength. This spectrophotometer is provided with WI lamp for visible range measurement and D2 lamp for ultraviolet range measurement. The wavelength is selectable with a range of 325.0 ~ 370.0 nm in increments of 0.1 nm.
WI Lamp: Turn on or turn off the WI lamp.
D2 Lamp: Turn on or turn off the D2 lamp.
Note: If turn on the lamp but display “OFF”, perhaps the lamp is error.
3.3.1.6 Save Parameter
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Press <6> key to select Save Parameter from the photometry submenu. The submenu screen is shown as following:
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Photometry
1 Parameter Setup
2 Sample Setup
3 Curve Setup
4 Print Setup
5 System Setup
6 Save Parameter
0 Forward
Select Function: _
Use the keypad to select the desired catalog number (1-20), then press ENTER. If the memory location that you have selected is available, the instrument stores entire variable set under test name you have specified. If the catalog number you requested is already full, the following message appears: Overwrite this File? To delete the previous test variables and store the new variable set, press ENTER. To leave the old variables set intact and store new variables in a different location, press RETURN.
3.3.2 Performing Photometric Measurements
Once the appropriate operating conditions are selected, the system is ready for measurement. 1. “%T” or “ABS” selected for Data Mode Press <0> on the keyboard and the variable selection screen will change to the measurement screen and the following message will appear on the screen: Insert SAMP and Press Start to Measure: Press Autozero on the keyboard to begin an autozero first, and then insert the sample and press START key to begin a photometric measurement. The results
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of measurement (value of absorbance or transmittance) will display on the screen. After finishing the measurement, press Clear/Return key to go on other performance.
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Photometry
ID
12
ABS(T%)
Select Function:_
0.1030.108
If you press CLEAR key right after the measurement without save, such hint will appear on the screen: Data Save? Yes: Clear key/ No: Enter key. To save the measurement, press Clear/Return key and return the last display. Then select the memory where you want to save the file (Inside memory or USB). If you do not want to save the measurement result, press Enter key directly.
2. “Conc” selected for Data Mode
Select “Conc” data mode from item “Parameter Setup”. After parameter being set up, press <0> on the keyboard and the variable selection screen will change to the measurement screen on which is shown in the illustration below.
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Press Autozero on the keyboard to begin an autozero, and then insert the and
1 Measure 2 Save Inside 3 Save to USB
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Photometry
STD ABS Conc
Insert STD and Press Start to Measure
standard sample 1 and press START. The standard sample 1 is measured the resultant value is indicated on the screen. In the same manner, repeat measurement as many time as the specified number of standards. Upon completion of standard sample measurements, a calibration curve is dispon the right side of the screen on which is shown in the illustration below.
s, a calibration curve is dispon the right side of the screen on which is shown in the illustration below.
layed
layed
1 Measure 2 Save Inside 3 Save to USB 4 Graph 5 Blank 6 Statistic
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Photometry 0.410
STD ABS Conc
1 -.0068 0.000
2 -.0098 0.050
3 0.203 0.100 ABS
4 0.300 0.150
5 0.410 0.200 -0.0680 MG/L 0.200
Conc = f(ABS) K0 = 0.000 K1 = 0.577 R= 1.000 R2 = 1.000Select Function: _
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If the Repeat Num is more than one, all the measurement value will be displayed
fter measuring of standard sample, insert the unknown sample and then press
s
Note: The “+” marks indicating the measured points are suppressed inpresentation of a saved calibration curve.
in the screen. For example, the Repeat Num is 2, the following screen will appear on the LCD.
600.0 nm 0.854 ABS
Photometry0.410
STD ABS Conc
1-1 -0.0681-2 -0.0681 -0.068 0.000 ABS
2-1 0.098
2-2 0.100 0.050
2 0.099 -0.0680.000 MG/L 0.200
Conc = f(ABS) K0 = 0.000 K1 = 0.577 R= 1.000 R2 = 1.000Select Function: _1 Measure 2 Save Inside 3 Save to USB 4 Graph 5 Blank 6 Statistic
A<1> key on the keypad to measure the unknown sample. Press <2> to save the measurement results. The instrument can save 10 files (1 –10) at most. If you want to save the file in the flash memory, press <3> key on the keyboard. Pres<4> key on the keyboard to adjust the coordinate of calibration curve. The display screen is shown as follow after pressing <4> key.
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Press <1> key on the keypad to trace the absorbance. The absorbance at any nd
Press Return/Clear to return the last interface screen and then press <2> on the
1 Trace 2 Zoom 3 STD Data
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Photometry0.410
ABS
-0.068
0 0.200
Conc = f(ABS) K0 = 0.000 K1 = 0.577 R= 1.000 R2 = 1.000Select Function: _
wavelength will be displayed at the bottom of the screen by pressing the left aright arrow key. The display screen is shown as follow.
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Photometry0.410
ABS
-0.068
0 0.200
Conc = f(ABS) K0 = 0.000 K1 = 0.577 R= 1.000 R2 = 1.000Select Function: _Trace conc 0.0 ABS -0.068
keypad to adjust the graph by changing the value of the two axis. The adjusting screen is shown as following.
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Zoom0.410
1 X - Max 0.200
2 X- Min 0
3 Y- Max 0.410 ABS
4 Y- Min -0.068
-0.0680.000 MG/L 0.200
Select Function: _
Press <3> key to display the measurement data of standard sample. But it just displays the mean value of several results for the same standard sample even if it is measured more than one times. Then select “Remeasure” by pressing <1> on the keypad to remeasure the absorbance of this standard sample. Select the “Delete” to delete the result you don’t want.
1 Remeasure 2 Delete
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Photometry
STD ABS Conc1 -0.068 0.0
2 0.098 1.0
3 1.025 5.0
1-1 -0.068 2-2 -0.068
Select Function: _
If you need measure blank sample value, press <5> before measure sample. Once you press <5> key, the display will prompt you to insert the sample and press Start to measure and press Clear/Return to return the last menu. Then
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press <1> to start the sample measurement. Prompt --“Insert the sample and press START to measure”-- is displayed at the bottom of the screen. Then insert unknown samples and press START. The concentration calculation
If the sample were measured more than 1 times, you can press <6> to get
he values of “Mean”, “SD” and “CV” are calculated according the following
is performed automatically and the measured results will be displayed on the LCD. The measurement result will be displayed on the screen as follow.
Note: Press Autozero key to exert an autozero before measurement.
statistics. The display will be as following:
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Photometry0.098
ID ABS Conc
1 0.087 5.0
2 0.098 10.0
0.0870 MG/L 10.0
Select Function: _Mean=7.5 2 SD=3.535 3 CV=47.13
Tformula.
NXi
MEAN ∑=
1
2
−
⎟⎟⎠
⎞⎜⎜⎝
⎛−
=∑ ∑
N
Nx
x
SD
ii
100×=MEAN
SDCV
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Where
e concentration value of No.i = the number of samples
ion
sults are less than 2, the statistic function will not work.
Xi＝ThNMEAN = the mean value SD = Standard deviation CV = Coefficient of variat Note: If the measurement re
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3.4 Wavelength Scan
Select wavelength scan from the Main Menu. Press MAIN MENU on the keypad to enter the main menu screen. And then press 2 on the keyboard from the main menu to enter the Wavelength Scan sub menu which is shown in the illustration below.
11/04/02 9:54 600.0 nm 0.854 ABS
Wavelength Scan
1 Parameter Setup
2 Sample Setup
3 Print Setup
4 System Setup
5 Save Parameter
6 User Baseline
0 Forward
Select Function: _
3.4.1 Measurement Parameter Setting
When the sample is volatile liquid, please must cover the sample cell with lid. Don’t measure the combustible liquid or gas with this instrument.
Note:
1. Parameter Setup
Press <1> to select parameter setup from the Wavelength Scan Sub Menu and a sub menu list will appear on the right side of the screen as shown in the illustration below.
3-20
11/04/02 9:54 600.0 nm 0.854 ABS
Wavelength Scan
1 Parameter Setup 1 Data Mode ABS
2 Sample Setup 2 Start WL (nm) 600.0
3 Print Setup 3 Stop WL (nm) 500.0
4 System Setup 4 Up Scale 3.000
5 Save Parameter 5 Low Scale -3.0006 User Baseline 6 Scan Speed (nm/min) 400
0 Forward 7 Num Cycles 18 Cycle Time (s) 09 Display Format Sequential
Select Function: _
1 %T 2 ABS 3 E(S) 4 E( R)
Data Mode Select one of the following modes: a) %T (percent transmittance measurement) b) ABS (absorbance measurement) c) E (S) (Energy on the sample side measurement) d) E (R) (Energy on the reference side)
Start WL (nm)
Start Wavelength for wavelength scanning operation. Enter a long wavelength value of the measurement wavelength range. This parameter is specifiable within a range of 190 to 1100 nm in increments of 0.1 nm.
Stop WL (nm)
Stop Wavelength for wavelength scanning operation. Enter a short wavelength value of the measurement wavelength range. This parameter is specifiable within a range of 190 to 1100 nm in increments of 0.1 nm.
Note: 1. The minimum scan width is 10 nm.
2. The data read interval varies depending on the wavelength range. 3. The data reading wavelength interval varies depending on the
specified wavelength range.
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. r Y-axis upper limit in each Data Mode is as follows:
Low Scale (Lower Scale)
r Y-axis upper limit in each Data Mode is as follows:
Scan Speed (nm/min)
g speed. ues are selectable:
200; 7)2400; 8)3600 Cycle Num (Cycle numbers)
e times which the sample is measured after the
Sequential,you can select Num Cycles from 1 to 99.
Cycle Time
alue from 0 to 9999 second.
Display Format
options –Sequential and Overlay -- in this function. If the elected,
2. Sample Setup
ct sample setup from the Wavelength Sub Menu and a sub menu
Up Scale (Upper Scale) Upper limit on Y-axisThe specifiable range foABS: Within a range of –3.000 to 3.000 in increments of 0.001. %T: Within a range of 0 to 600.0in increments of 0.1.
Lower limit on Y-axis. The specifiable range foABS: Within a range of –3.000 to 3.000 in increments of 0.001. %T: Within a range of 0 to 600.0 in increments of 0. 1.
Wavelength scanninThe following eight speed val1) 10; 2) 100; 3) 200; 4) 400; 5) 800; 6)1
This parameter indicates thSTART key is pressed. When Display Format isWhen Display Format is Overlay, Num Cycle is fixed 2.
Input this v
There are two Sequential is selected, only the last graph is displayed. If the Overlay is sall the graph are displayed on the screen.
Press <2> to selelist will appears on the right side of the screen as shown in the illustration below. These parameters are the same as section 3.3.
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11/04/02 9:44 600.0 nm 0.854 ABS
Wavelength Scan
1 Parameter Setup 1 Name SAMP-1
2 Sample Setup 2 Path Length (nm) 10.0
3 Print Setup
4 System Setup
5 Save Parameters6 User Baseline0 Forward
Input Name:_
Name
ring Sample name. pecifiable. It can input “0-9”, “A-Z”, “-”, “/”, “:”.
m) t was preformed using a cell other than 10 mm rectangular
. Print Setup
elect system setup from the Wavelength Sub Menu and a sub menu
MeasuUp to eight characters are s Path length (mWhen measuremencell, the photometric value can be converted into one measured with a 10 mm rectangular cell.
3Press <3> to slist will appears on the right side of the screen as shown in the illustration below.
3-23
11/04/02 10:17 600.0 nm 0.854 ABS
Wavelength Scan
1 Parameter Setup 1 Printer Type ESC/P2 Sample Setup 2 Auto Print ON
3 Print Setup 3 Graph Print ON4 System Setup 4 Data Print Peak
5 Save Parameter 5 List Interval (nm) 10.06 User Baseline0 Forward
Select Function: _
Printer Type There are three kinds of printer to select: EPSON, Seiko, HP.
Auto Print If the autoprint function is “ON”, the DB-20 will print automatically the setting parameter and the graph or data after completion of a measurement. But if the autoprint function is “OFF”, the instrument will not print automatically unless the Print key is pressed.
Graph Print Using this parameter to determine whether to output the on-screen spectral graph onto the printer automatically at the end of wavelength scanning.
Data Print If it is desired to print out photometric values at a fixed wavelength interval during spectral scanning, set the Text Print parameter to Peak, Valley or All Data. Otherwise, set this parameter to Not Print.
List Interval (nm) If the Text Print parameter is set to ON, specify a wavelength interval at which photometric values are printed out. This parameter is specifiable within a range of 0.1 to 100.0 nm in increments of 0.1 nm.( Please refer to section 3.4.4 )
4. System Setup
Press <4> to select system setup from the Wavelength Sub Menu and a sub menu list will appears on the right side of the screen as shown in the illustration below. These parameters are the same as 3.3 except Peak Threshold.
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11/04/02 11:23 600.0 nm 0.854 ABS
Wavelength Scan
1 Parameter Setup 1 Auto
2 Sample Setup 2 Lamp Change WL (nm) 350.0
3 Print Setup 3 WI Lamp On
4 System Setup 4 D2 Lamp On
5 Save Parameter 5 Peak Threshold 0.0016 User Baseline 6 Baseline System
0 Forward 7 Response Medium
8 Init Delay (s) 0
Select Function: _
Lamp Change Mode
1 Auto 2 D2 Only 3 WI Only
Lamp Change Mode: The followings three Lamp Change Mode are available. Auto: Changeover is automatically made between D2 Lamp and WI Lamp. D2 Lamp Only: Using D2 lamp over the entire wavelength region. WI Lamp Only: Using WI lamp over the entire wavelength region.
Lamp Change WL (nm): This parameter is used for selection of light source switching wavelength. This spectrophotometer is provided with WI lamp for visible range measurement and D2 lamp for ultraviolet range measurement. The wavelength is selectable with a range of 325.0 ~ 370.0 nm in increments of 0.1 nm.
WI Lamp: Turn on or turn off the WI lamp. D2 Lamp: Turn on or turn off the D2 lamp.
Peak Threshold:
Threshold function is used to determine the level of signal changes required to be identified as peak or valley. The range of threshold is 0.1 ~ 100.0 %T（E） or 0.001 ~ 1.000 ABS.
Baseline: Choose the kind of baseline to use for measurement. The following four kinds of baselines are selectable: a) System (auto calibration from 190 to 1100 nm)
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b) User 1 (calibration according to input conditions) c) User 2 (calibration according to input conditions) d) None There is no baseline.
Response: There are three response speeds to select: Fast, Medium and Slow. In the ordinary measurement, select “Medium”. If it is desired to reduce variation, choose the slow response parameter. If it is required carry out high-resolution measurement, select “Fast” response parameter.
Init Delay: Specify an initial delay time where it is required to wait until the temperature becomes stable for sample measurement or the sample sipper is employed. In an ordinary measurement application, set an initial delay time to ‘0’ (zero). This parameter is specifiable within a range of 0 to 9999 seconds in increments of one second.
5. Save Parameter
Press <5> to select Save Parameter from the wavelength scan submenu, a submenu list appears on the right side of the screen as shown in the illustration below:
10/11/02 13:28 600.0 nm 0.854 ABS
Wavelength Scan
1 Parameter Setup2 Sample Setup3 Print Setup
4 System Setup
5 Save Parameter
6 User Baseline
0 Forward
Input File No.:_(1 to 20)
Use the keypad to select the desired catalog number (1-20), then press ENTER. If the memory location that you have selected is available, the instrument stores entire variable set under test name you have specified. If the catalog number you requested is already full, the following message appears: Overwrite this File?
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To delete the previous test variables and store the new variable set, press ENTER. To leave the old variables set intact and store new variables in a different location, press RETURN.
6. User Baseline
Press <6> to select User Baseline from the wavelength scan submenu, a submenu list appears on the right side of the screen as shown in the illustration below:
09/11/02 15:32 600.0 nm 0.854 ABS
Wavelength Scan
1 Parameter Setup 1 System
2 Sample Setup 2 User 1
3 Print Setup 3 User 2
4 System Setup5 Save Parameter
6 User Baseline
0 Forward
Press START to measure or Return to exit
Press the numeric key to select System, User 1 or User 2. With the screen displayed, press START and the baseline measurement is executed. The wavelength range of the User 1 and User 2 is specified under the parameter Setup. The user baseline measurement is conducted under the currently defined conditions. Therefore, efficient correction can be made using the identical conditions (wavelength range, scan speed, data read interval, etc.) to those of actual measurement. Note: If the baseline is out of baseline range you selected or there is not
baseline stored in memory, such hint will appear on the bottom of screen.
Baseline out of Range
7. Forward: End setting up and go to the measurement display.
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3.4.2 Performing a Wavelength Measurement
Upon completion of measurement conditioning setting, press the <0> key and the measurement screen appears on the LCD. When the conditioning screen is switched over to the measurement screen, the instrument checks the user-specified parameters. If any conditional inconsistency is found, the relevant error message is displayed at the bottom of the screen. Also, if the currently wavelength does not match the start wavelength, it is forced to shift to the level of start wavelength. Press Autozero key on the keyboard to execute an auto zero. Then set a sample and press the START key to perform wavelength scanning and the spectrum will be displayed on the screen. To abort a measurement during operation, press the STOP key. Upon completion of measurement, the following spectral plot appears on the LCD. If the Auto Print and Graph parameter is preset to “On”, the on-screen spectral plot is output to the printer if connected. If the printer does not print the graph correctly, check the printer setup menu to make sure that the correct printer type is selected.
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Wavelength Scan
3.000
ABS
-3.000190 1100.0
Select Function:_1 Trace 2 Peak 3 Zoom 4 Process 5 Reset 6 File
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3.4.3 Secondary Processing
The measured spectrum is stored in internal memory. Several kinds of computational processing can be performed for the stored spectrum. The following explains these secondary-processing operations. Note: The second processing is effective only for the spectral data measured last. For the overlaid spectral attained through repetitive scanning, only the spectral data measured in last cycle can be handled in the secondary processing. (a) Trace The Trace processing function allows the user to read out values of the on-screen spectrum by means of the crosshair “+” cursor. On completion of measurement, press <1> on the keyboard and then the trace screen appears as shown below.
11/08/02 16:44 600.0 nm 0.854 ABS
Wavelength Scan
3.000
ABS
-3.000190 1100.0
Select Function:_Trace: nm 500.0 ABS 0.043
When the crosshair (+) cursor appears on the spectral graph, it can be moved left/right using the arrow keys. The wavelength range that the cursor moved each time is according to Wavelength Range on Interval. Holding the arrow key down causes this cursor to move quickly. The wavelength and photometric value pointed by the cursor are read out and indicated at the bottom of screen.
(b) Peaks
The Peaks processing function allows the user to detect spectral peaks. On completion of measurement, press <2> and then the peaks screen is presented as shown below.
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11/08/02 16:44 600.0 nm 0.854 ABS
Wavelength Scan
3.000
ABS
-3.000190 1100.0
Select Function:_1 Peak 2 Valley 3 All Data
If you want to read the value of peaks, press <1> and the graph screen will change to the data screen as below. Similarly, press <2> and the value of the valleys will be list on the screen.
11/05/02 15:23 600.0 nm 0.854 ABS
Peaks
ID WL(nm) ABS ID WL(nm) ABS ID WL(nm) ABS1 512 0.172 2 548 0.068 3 589 -0.061
Select Function:_
If you want all data listed on the screen, then press <3> on the keypad. Press Return key to return last interface.,
(c) Zoom
This function allows the user to rescale the measured spectrum in different graph axial scales.
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After measurement, press <5> on the keyboard and then the rescale screen is presented as shown below.
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Zoom
1 X - Max 600.0
2 X- Min 500.0
3 Y- Max 3.000
4 Y- Min -3.000
Select Function: _
(d) Process
This function is used for data processing. Press <4> , and the measurement screen change to the process screen as follow.
1 Smooth 2 Deriv 3 Area 4 Calcu 5 +K 6 *K 7 Reset
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Wavelength Scan
3.000
ABS
-3.000190 1100.0
Select Function:_
Smooth
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The smooth function employs a data-smoothing algorithm to smooth the noise. DB-20 can displays 1st through 4th order smooth spectrum. Selection of this function causes the spectral graph screen to change.
Deriv
The Deriv function allows the user to obtain a derivative spectral graph. DB-20 can displays 1st through 4th order derivate spectrum.
Area
This function is used to carry out area calculation. Specify the wavelength range for area calculation, and this function will perform integration. When the spectral wavelength Start wavelength and End wavelength are specified, the Area function calculates the following area values.
Calcu (Calculation)
Selection of this function causes the spectral graph screen to change as shown below.
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Calc
1 File A 1
2 File B 2
3 Arithmetic A + B
Select Function or Press Start to Calculate
File A or File B is to select stored file. Item 3 (Arithmetic) is to select calculation function such as ‘A+B’, ‘A-B’, ‘A*B’, ‘A/B’. Addition, subtraction, multiplication, and division can be performed on 2 sets of spectral data.
+K (or * K)
The +K (*K) option performs addition (multiplication) of constant (K) on the spectral plot. A constant can be entered in four digits within a range of –9999 to 9999.
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(e) Reset Using this function allows you to load the originality spectrum
(f) Files
DB-20 is capable of storing the spectral or time scan graph data in internal memory. Press <6> to save or load the spectral or time scans data.
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W avelength Scan
3.000
ABS
-3.000190.0 1100.0
Select Function:_
1 Save 2 Load 3 Overlay
Save: Save the spectrum as ‘1’ to ‘10’. Load: Load the stored file ‘1” to ‘10’. Overlay: Overlap the spectral data of the specified file on the current spectral graph.
3.4.4 Wavelength Scan Sampling Interval List Wavelength scan sampling interval (Unit: nm) as following List.
Scan range (1-100)
nm (100.1-200) nm
(200.1-500) nm
(500.1-910) nm
3600 5 5 5 5 2400 5 5 5 5 1200 2 2 2 2 800 1 1 1 1 400 0.5 0.5 0.5 1 200 0.2 0.2 0.5 1 100 0.1 0.2 0.5 1 10 0.1 0.2 0.5 1
Velocity
(nm/min)
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3.5 Time Scan
Select wavelength scan from the Main Menu. Press 3 on the keyboard to enter the Wavelength Scan sub menu
11/10/02 9:47 600.0 nm 0.854 ABS
Time Scan
1 Parameter Setup 1 Data Mode ABS
2 Sample Setup 2 WL (nm) 600.0
3 Print Setup 3 Scan Time (s) 500
4 System Setup 4 Up Scale 3.000
6 Save Parameter 5 Low Scale -3.000
0 Forward
Select Function: _
Note: When the sample is volatile liquid, pleas must cover the sample cell with lid. Don’t measure the combustible liquid or gas with this instrument.
3.5.1. Measurement Parameter Setting
1. Parameter Setup Press <1> to select parameter setup from the Time Scan Sub Menu and a sub menu list will appears on the right side of the screen as shown in the illustration below.
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11/10/02 9:47 600.0 nm 0.854 ABS
Time Scan
1 Parameter Setup 1 Data Mode ABS
2 Sample Setup 2 WL (nm) 600.0
3 Print Setup 3 Scan Time (s) 500
4 System Setup 4 Up Scale 3.000
6 Save Parameter 5 Low Scale -3.000
0 Forward
Select Function: _
1 %T 2 ABS 3 E(S) 4 E(R)
Data Mode
Select one of the following modes: a) %T (percent transmittance measurement) b) ABS (absorbance measurement) c) E (S) (Energy on the sample side measurement) d) E (R) (Energy on the reference side)
WL (nm) (Wavelength) Enter a wavelength to be used for measurement. DB-20 has a range of 190 to 1100 nm in increments of 0.1 nm.
Scan Time
Specify a scanning time which corresponds to the upper limit value on the abscissa axis of spectral graph. Range of 60 to 99999 seconds in increments of one second.
Up Scale (Upper Scale)
Upper limit on Y axis. Range for Y-axis upper limit in each Data Mode is as follows: ABS: Within a range of –3.000 to 3.000 in increments of 0.001. %T: Within a range of 0 to 600.0 in increments of 0. 1.
Low Scale (Lower Scale)
Lower limit on Y-axis. Range for Y-axis upper limit in each Data Mode is as follows: ABS: Within a range of –3.000 to 3.000 in increments of 0.1.
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%T: Within a range of 0 to 600.0 in increments of 0. 1.
Response Three response speeds are available: Fast, Medium and Slow. Most application requires a “Medium”. For better reproducibility, choose the slow response parameter. For high-resolution measurement, select “Fast” response parameter.
Note: The data reading time interval varies depending on the specified scan time.
Determine a period of scan time according to the purpose of measurement. As the scan time increase, data is smoothed more to reduce noise.
Scan Time (sec) Reading Interval (sec) Scan time < 100 0.1
100 ≤ Scan time < 200 0.2 200 ≤ Scan time < 500 0.5 500 ≤ Scan time < 1000 1.0 1000 ≤ Scan time < 2000 2.0 1000 ≤ Scan time < 5000 5.0 5000 ≤ Scan time < 9999 10.0
Scan time ≥ 10000 100.0 2. Sample Setup
Press <2> to select sample setup from the Time Scan Sub Menu and a sub menu list will appears on the right side of the screen as shown in the illustration below. These parameters are the same as 3.3.
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Time Scan
1 Parameter Setup 1 Name SAMP-1
2 Sample Setup 2 Path Length (nm) 10.0
3 Print Setup
4 System Setup
5 Save Parameter
0 Forward
Input Name:_
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Name
Sample name. Up to eight characters are specifiable. It can input “0-9”, “A-Z”, “-”, “/”, “:”.
Path length (mm)
When measurement was preformed using a cell other than 10 mm rectangular cell, the photometric value can be converted into one measured with a 10 mm rectangular cell.
3. Print Setup
Press <3> to select system setup from the Wavelength Sub Menu and a sub menu list will appears on the right side of the screen as shown in the illustration below.
11/10/02 13:29 600.0 nm 0.854 ABS
Time Scan
1 Parameter Setup 1 Printer Type ESC/P
2 Sample Setup 2 Auto Print ON
3 Print Setup 3 Graph Print ON
4 System Setup 4 Data Print Peak
5 Save Parameter 5 List Interval (nm) 10.0
0 Forward
Select Function: _
Printer Type
There are three kinds of printer to select: EPSON, Seiko, HP. Auto Print
If the Auto print function is “ON”, the DB-20 will print automatically the setting parameter and the graph or data after completion of a measurement. But if the Auto print function is “OFF”, the instrument will not print automatically.
Graph Print Using this parameter to determine whether to output the on-screen spectral graph onto the printer automatically at the end of wavelength scanning.
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Data Print If it is desired to print out photometric values at a fixed wavelength interval during spectral scanning, set the Text Print parameter to Peak, Valley or All Data. Otherwise, set this parameter to Not Print.
List Interval (nm) If the Text Print parameter is set to ON, specify a wavelength interval at which photometric values are printed out. This parameter is specifiable within a range of 0.1 to 100.0 nm in increments of 0.1 nm.
4. System Setup
Press <4> to select system setup from the Time Scan Sub Menu and a sub menu list will appears on the right side of the screen as shown in the illustration below. These parameters are the same as 3.3 except Peak Threshold. Refer to the System Setup of Wavelength Scan in Section 3.4.
1 Auto 2 D2 Only 3 WI Only
11/10/02 14:25 600.0 nm 0.854 ABS
Time Scan
1 Parameter Setup 1 Auto
2 Sample Setup 2 Lamp Change WL (nm) 350.0
3 Print Setup 3 WI Lamp On
4 System Setup 4 D2 Lamp On
5 Save Parameter 5 Peak Thershold 0.001
0 Forward 6 Baseline System
7 Response Medium
8 Init Delay (s) 0.1
Select Function: _
Lamp Change Mode
3.5.2 Performing a Time Scan measurement
On completion of measurement conditioning setting, press the <0> key and the measurement screen appears on the LCD. When the conditioning screen is switched over to the measurement screen, the instrument checks the user-specified parameters.
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If any conditional inconsistency is found, the relevant error message is displayed at the bottom of the screen. Also, if the currently specified wavelength does not match the measurement wavelength, it is forced to shift to the level of measurement wavelength. Press Autozero key on the keyboard to execute an auto zero. Then set a sample and press the START key. Thus, time scanning is carried out and the spectrum is displayed on the screen. To abort measurement prematurely, press the STOP key. On completion of measurement, the following spectral plot appears on the LCD. If the Auto Print and Graph Print parameter is preset to “ON”, the on-screen spectral plot is output onto the printer also.
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Time Scan
1.000
ABS
0.0000 60s
Select function: _
1 Trace 2 Peak 3 Zoom 4 Process 5 Reset 6 File
3.5.3 Secondary Processing
The measured spectrum is stored in internal memory. Several kinds of computational processing can be performed for the stored spectrum. The following explains these secondary-processing operations. Note: The second processing is effective only for the spectral data measured last. For the overlaid spectral attained through repetitive scanning, only the spectral data measured in last cycle can be handled in the secondary processing.
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(a) Trace The Trace processing function allows the user to read out values of the on-screen spectrum by means of the crosshair “+” cursor. On completion of measurement, press <1> on the keyboard and then the trace screen appears as shown below.
11/11/02 10:41 600.0 nm 0.854 ABS
Time Scan
1.000
ABS
0.0000 60s
Trace :S 12 ABS 0.031
When the crosshair (+) cursor appears on the spectral graph, it can be moved left/right using the arrow keys. Holding the arrow key down causes this cursor to move quickly. The time and photometric value pointed by the cursor are read out and indicated at the bottom of screen. (b) Peaks The Peaks processing function allows the user to detect spectral peaks. On completion of measurement, press <2> and then the peaks screen is presented as shown below.
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11/11/02 10:41 600.0 nm 0.854 ABS
Time Scan
1.000
ABS
0.0000 6
Select Function:_0s
1 Peak 2 Valley 3 All Data 4 Kinetics
Press <1> and the screen will change the peak data screen as shown below. Similarly, press <2> and the Valley data will be listed on the screen. If you press <3> key and all data will be listed on the screen.
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Peak
ID Time(s) ABS ID Time(s) ABS ID ABS
No Peaks
Selection Function :_
Time(s)
Press <4> key to select Kinetics. Then, you can input the start time and end time. The screen will change to Kinetics screen as shown below.
3-41
11/10/02 17:49 600.0 nm 0.85 ABS
Kinetics
Start(s) Stop(s) Slope(ABS/min) R R2
10 30 -6.6725E-05 0.170 0.029
Selection Function :_
(c) Zoom This function allows the user to rescale the measured spectrum in different graph axial scales. After measurement, press <3> on the keyboard and then the rescale screen is presented as shown below. Press the numeric key to adjust the range of X-axis and Y-axis.
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Zoom
1 X - Max 60.0
2 X- Min 0
3 Y- Max 0.100
4 Y- Min 0.000
Select Function: _
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(d) Process
This function is used for data processing. Press <4>, and the measurement screen change to the process screen as follow.
Smooth
The smooth option is used for time scan graph smoothing. This function is convenient when handling a spectral graph containing appreciable noise. DB-20 can displays 1st through 4th order smooth spectrum..
Deriv
The Deriv function allows the user to obtain a derivative spectral graph. Press <2> to select this function causes the spectral graph screen to change as shown below. DB-20 can displays 1st through 4th order smooth spectrum.
Area This function is used to carry out area calculation. Specify the time range for area calculation, and this function will perform integration. When the spectral time Start wavelength and End time are specified, the Area function calculates the following area values.
Calcu Selection of this function causes the spectral graph screen to change as shown below.
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Calc
1 File A 1
2 File B 2
3 Arithmetic A + B
Select Function or Press Start to Calculate
3-43
File A or File B is to select stored file. Item 3 (Arithmetic) is to select calculation function such as ‘A+B’, ‘A-B’, ‘A*B’, ‘A/B’. Using these function, addition, subtraction, multiplication, and division can be performed on spectral data.
+K (or * K) The +K (*K) option performs addition (multiplication) of constant (K) on the spectral plot. A constant can be entered in four digits within a range of –9999 to 9999.
(e) Reset
Using this function allows you to load the originality spectrum
(f) Files DB-20 is capable of storing the spectral or time – varying graph data in internal memory. After memory, press <6> and the spectral screen is changed as shown below.
Save: Save the spectrum as ‘1’ to ‘10’.
e specified file on the current spectral
5. Save Parameter
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Time Scan
1.000
ABS
0.0000 60s
Select Function:_1 Save 2 Load 3 Overlay
Load: Load the stored file ‘1” to ‘10’. Overlay: Overlap the spectral data of thgraph.
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Press <5> to select Parameter Setup from the time scan submenu, and a submenu list will appear on the right side of the screen as shown in the illustration below:
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Time Scan
1 Parameter Setup
2 Sample Setup
3 Print Setup
4 System Setup
5 Save Parameter
0 Forward
Input File No._(1 to 20)
Use the keypad to select the desired catalog number (1-20), then press ENTER. If the memory location that you have selected is available, the instrument stores entire variable set under test name you have specified. If the catalog number you requested is already full, the following message appears: Overwrite this File? To delete the previous test variables and store the new variable set, press ENTER. To leave the old variables set intact and store new variables in a different location, press RETURN.
6. Forward: End setting up and go to the measurement display.
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3.6 Multiple WL
Note: When the sample is volatile liquid, pleas must cover the sample cell with lid. Don’t measure the combustible liquid or gas with this instrument.
Press [4] on the keyboard to enter the Multiple WL option from the main menu. The Multiple WL submenu will be displayed on the LCD as shown in the illustration below.
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Multipl WL
1 Parameter Setup
2 Sample Setup
3 Curve Setup
4 Print Setup
5 System Setup
6 Save Parameter
7 User Baseline
0 Forward
Select Function:_
3.6.1 Measurement Conditions Setting
1. Parameter Setup Press <1> to select parameter setup from the Multiple WL Sub Menu and a sub menu list will appears on the right side of the screen as shown in the illustration below. Press numeric key to select corresponding function.
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11/12/02 15:38 600.0 nm 0.854 ABS
Multipl WL
1 Parameter Setup 1 Data Mode ABS
2 Sample Setup 2 NUM WL 2
3 Curve Setup 3 WL Setup 500.0
4 Print Setup 4 Baseline System
5 System Setup
6 Save Parameter
7 User Baseline
0 Forward
Select Function:_1 T% 2 ABS 3 Conc 4 Ratio
Data Mode Select one of the following modes: a) %T (percent transmittance measurement) b) ABS (absorbance measurement) c) Conc (concentration measurement, 2-, 3-wavelength calculation)
Concentration measurement only allows max 3 wavelengths. d) Ratio
Ratio of 2 wavelengths can be performed. Example of application includes purity determination of DNA and/or RNA by using the difference and ratio of the absorbance at two user-selected wavelengths. Background can be corrected at a specific wavelength if the NUM WL is set at 3.
WL NUM
When NUM WL is selected on the Multiple WL condition-setting screen, the following screen appears on the LCD.
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11/12/02 15:38 600.0 nm 0.854 ABS
Multipl WL
1 Parameter Setup 1 Data Mode ABS
2 Sample Setup 2 NUM WL 2
3 Curve Setup 3 WL Setup 500.0
4 Print Setup 4 Baseline System
5 System Setup
6 Save Parameter
7 User Baseline
0 Forward
Input Value:_(2 to 6)
Specifies the number of wavelengths. The following shows the relation between the number of wavelengths and each data mode. Data Mode
Settable Wavelengt
h
Description
T% 2 to 6 Transmittance at each wavelength is measured
and displayed. ABS 2 to 6 Absorbance at each
wavelength is measured and displayed.
2 2–wavelength calculation is made and concentration
is obtained.
WL
Conc
3 3–wavelength calculation is made and concentration
is obtained.
(a) WL NUM (Data Mode: Conc) The photometric values A (WL1) and A (WL2) at two specified wavelength is calculated based for the following formula. D = WL2 – WL1
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A(WL1)A(WL2)Ratio =
(b) NUM WL ‘3’ (Data Mode: Conc) The photometric values A (WL1), A (WL2) and A (WL3) at three specified wavelengths are calculated base on the following formula. D = (WL2 – WL3) – (WL1 – WL3)
A(WL3) - A(WL1)
A(WL3) - A(WL2)Ratio =
(c) When NUM WL Ranges from 2 to 6 (Data Mode: ABS or T%) When data mode is ABS (or T%), absorbance (or transmittance) at each set wavelength is displayed.
WL Setup
Setting the wavelength. Press <3> on the keyboard to select this function and the setting screen will change as following.
11/12/02 15:49 600.0 nm 0.854 ABS
Multipl WL
1 Parameter Setup WL Setup (nm)
2 Sample Setup WL1(nm) 200.0
3 Curve Setup WL2(nm) 300.0
4 Print Setup WL3(nm) 400.0
5 System Setup
6 Save Parameter
7 User Baseline
0 Forward
Input Value :_(190.0 to 1100.0)
Baseline
Press <4> key on the keyboard to select appropriate baseline type. Four options are available: System, User1, User2 and None. System baseline is recommended for most applications.
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2. Sample Setup(refer to section 3.3) When selecting the Sample Setup, the Sample Setup screen appears on the LCD as shown below.
11/13/02 9:54 600.0 nm 0.854 ABS
Multiple WL
1 Parameter Setup 1 Name SAMP-1
2 Sample Setup 2 Path Length (nm) 10.0
3 Curve Setup 3 Unit Label MG/L
4 Print Setup
5 System Setup
6 Save Parameter
7 User Baseline
0 Forward
Input Value:_
3. Curve Setup (refer to section 3.3) Press <3> key to select Curve Setup from the Multiple WL submenu. A submenu list appears on the right side of the screen, four options are available.
1 1st Order 2 Second Order 3 Segment 4 K-factor
10/11/02 14:28 600.0 nm 0.854 ABS
Multiple WL
1 Parameter Setup 1 Curve Type 1st order
2 Sample Setup 2 STD NUM 3
3 Curve Setup 3 Curve Data
4 Print Setup 4 Repeat NUM 2
5 System Setup 5 Hi Limit 9999
6 Save Parameter 6 LO Limit 0
7 User Baseline
0 Forward
Select Function: _
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Curve Type: Select the required curve types. a) 1st order b) 2nd order c) Segment d) K – factor
Press corresponding key on the keyboard to select curve type. For “1st order”, the standards number should be more than 2. For “Second order”, the standards number should be more than 3. For “K-factor”, value of k0, k1 and k2 need to be input manually. Note: If the standards number is not fit the curve type, the system will automatically changed the numbers to fit the curve type. Curve Data: Input the standards concentration. Press <2> on the
keyboard to input the standard Curve Data. STD NUM: (Number of standards) Select the numbers of standards sample between “1” to “20”.
4. Print Setup (refer to section 3.3)
Press <4> to select Print Setup from the Multiple WL submenu, a submenu list appears on the right side of the screen as shown in the illustration below:
1 ESC/P 2 DPU 3 PCL3
11/13/02 11:35 600.0 nm 0.854 ABS
Multiple WL
1 Parameter Setup 1 Printer Type ESC/P
2 Sample Setup
3 Curve Setup4 Print Setup
5 System Setup
6 Save Parameter
7 User Baseline
0 Forward
Select Function: _
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Press the number key on the keyboard to select corresponding function.
rinter setup: DB-20 support three types of printer, namely ESC/P, PCL3 type
. System Setup (refer to section 3.3)
Press <5> to select System Setup from the Multiple WL submenu, a submenu
Lamp Change Mode: three Lamp Change Mode are available.
p and
nly: Using D2 lamp over the entire wavelength
p Only: Using WI lamp over the entire wavelength
Lamp Change WL (nm):
sed for selection of light source switching
hotometer is provided with WI lamp for visible
Pand Seiko thermal printer.
5
list appears on the right side of the screen as shown in the illustration below:
11/13/02 11:35 600.0 nm 0.854 ABS
Multiple WL
1 Parameter Setup 1 Auto
2 Sample Setup 2 Lamp Change WL (nm) 350.0
3 Curve Setup 3 WI Lamp On
4 Print Setup 4 D2 Lamp On
5 System Setup
6 Save Parameter
7 User Baseline
0 Forward
Select Function: _
Lamp Change Mode
1 Auto 2 D2 Only 3 WI Only
The followings Auto: Changeover is automatically made between D2 LamWI Lamp. D2 Lamp Oregion. WI Lamregion.
This parameter is uwavelength. This spectroprange measurement and D2 lamp for ultraviolet range
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measurement. The wavelength is selectable with a range of 325.0 ~ 370.0 nm in increments of 0.1 nm.
WI Lamp: Turn on or turn off the WI lamp.
D2 Lamp: Turn on or turn off the D2 lamp.
3.6.2 Performing Multiple WL Measurement
Creating a Standard Curve After setting the appropriate operating conditions, use the following steps to perform multiple wavelength measurements. 1. Press <0> on the keyboard. The condition-setting screen will switch to the measurement screen. 2. Press <Autozero> to start an autozero measurement. 3. Insert the first standard sample in the sample compartment. Then press Start to start measurement. Repeat the step, as many times as are required to analyze the number of standards after completing analysis of standard samples, the measurement screen will change to following screen.
1 Measure 2 Save Inside 3 Save to USB 4 Graph 5 Blank 6 Statistic
16:42 400.0 nm 0.457 ABS
Multiple WL
STD WL1 WLDiff Conc
(200nm) (300nm)
1 0.000 0.00.000 0.0002 0.098 0.10.016 0.0053 0.203 0.20.017 0.100
Select Function: _
11/13/02
Press <3> key on the keypad to select Graph. Then the new curve will be created as following.
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11/10/02 17:54 600.0 nm 0.854 ABS
Zoom0.410
1 X - Max 0.200
2 X- Min 0
3 Y- Max 0.410 ABS
4 Y- Min -0.068
-0.0680.000 MG/L 0.200
Select Function: _
The abscissa is the concentration and the ordinate is difference of ABS. Note: The “+” marks indicating the measured points.
Saving a Standard Calibration Curve After creating a new standard curve, press Clear/Return to return the measurement screen. Then press <2> to save the test. Measuring the Unknown Sample After saving the test, press <1> on the keyboard. The prompt “Insert Sample and Press Start to Measure or Return to exit” will appear on the bottom of the screen. Then, insert a sample and press Start. Repeat the procedure for multiple sample measurement. The measurement results will be displayed on the screen.
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3.7 File Manager
Press [5] on the keyboard to enter the File Manager option from Main menu. The File Manager submenu will be displayed on the LCD as shown below.
11/10/02 9:47 600.0 nm 0.854 ABS
File Manager
1 Inside Memory
2 Flash Memory
Select Function: _
You can save and load the test method in both inside memory and flash memory (normally refered as USB). Press relevant numeric key on keypad to select desired memory.
3.7.1 Inside Memory
Test method can be retrieved from the non-volatile memory: 1) Press <1> on the keyboard. Test names and test number will display on the
right side of the screen as follow.
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11/10/02 9:47 600.0 nm 0.854 ABS
File ManagerFile
1 Inside Memory 1 F1 UTT2 Flash Memory 2 F2 UTT
3 F3 UTS4 F4 UTQ5 F5 UTQ6 F6 UTT7 F7 UTS8 F8 UTS9 F9 UTT
10 F10 UTQ
Select Function: _
1 Open 2 Delete 3 Save to others 4 Rename
2) Select file name by pressing the Up and Down Arrow key from the keyboard.
3) ing function such as open, Delete
save the
3.7.2 Flash Memory
Press <1> key on the keyboard. Test names and test number will display on the
“.UTS” means wavelength scan test, “.UTT” means time scan test, “.UTQ” means photometry or multiple WL test. Press the numeric key to select correspondand so on. Press <1> to open the file and the display will be back the measurement screen before. Press <2> to delete the file. Press <3> to file in the flash memory. Press <4> to change the file name.
right side of the screen as follow.
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11/10/02 9:47 600.0 nm 0.854 ABS
File ManagerFile
1 Inside Memory 1 F1 UTT 112 Flash Memory 2 F2 UTT 12
3 F3 UTS 134 F4 UTQ 14
5 F5 UTQ 156 F6 UTT 167 F7 UTS 178 F8 UTS 189 F9 UTT 19
10 F10 UTQ 20
Select Function: _1 Open 2 Delete 3 Save to others 4 Rename
imilar to section 3.7.1, selecting test name by pressing the Up and Down ing
em
SArrow key from the keyboard and to select corresponding function by pressnumeric key. The number of file in flash memory is more than 400, using Left/Right arrow key page up/down. (Note: when using flash memory, PC software can open the files, such as UTT, UTS, UTQ, and also can export thto EXCEL report by PC computer .)
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3.8 Calibration
Press [6] on the keyboard to enter the Calibration mode. The Calibration submenu will be displayed on the LCD as shown below.
11/12/02 10:52 600.0 nm 0.854 ABS
Calibration
1 WL Calibration
2 Set Time
3 Lamp Time
4 GLP/GMP
Select Function: _
3.8.1 Calibrating the Wavelength
The entire calibration procedure is automatic and uses the 656.1 nm emission lines from the deuterium lamp. Use the following steps to calibrate the wavelength. 1. Press <1> on the keyboard. 2. Ensure that the cuvette holders are empty and the Sample Compartment is
closed. Press Start.
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11/15/02 11:15 600.0 nm 0.854 ABS
Calibration
1 WL Calibration2 Set Time
3 Lamp Time4 GLP/GMP
Are you sure to excute?
3.8.2 Set Time
If the time displayed on the LCD screen is incorrect, reset the clock using the following steps. Press <2> key on the keyboard from the Calibration submenu. Then the time setting submenu appears on the right of the screen as shown below.
11/15/02 11:15 600.0 nm 0.854 ABS
Calibration
1 WL Calibration 1 Minute 15
2 Set Time 2 Hour 11
3 Lamp Time 3 Day 15
4 GLP/GMP 4 Month 11
5 Year
6 Clock ON7 M/D/Y
Select Function: _
Display Format
1 Y/M/D 2 M/D/Y 3 D/M/Y
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1. Press the corresponding numeric key to select appropriate variables. 2. Select value you wish to enter, using the number key on the keyboard. Then
press Enter.
Note: The Date and Time data are non-volatile memory even power off. The memory will lose if the on board battery is out of power.
3.8.3 Recording Lamp Usage
Press <3> on the keyboard from the Calibration submenu, time setting submenu will appear on the right of the screen as shown below.
11/16/02 15:25 600.0 nm 0.854 ABS
Calibration
1 WL Calibration 1 Change New WI Lamp
2 Set Time 2 Change New D2 Lamp
3 Lamp Time WI Lamp Usage (h) 10
4 GLP/GMP D2 Lamp Usage (h) 10
Select Function: _
The Lamp Time setting keeps a record of how many hours of the D2 or WI lamp has been used. The memory is non-volatile and will stay in the memory even after power off. After replacing a new WI lamp or D2 lamp, press <1> or <2> to reset time of WI lamp or D2 lamp to zero.
3.8.4 GLP/GMP
Press <4> key on the keyboard from the Calibration submenu. Then the time setting submenu appears on the right of the screen as shown below.
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11/16/02 15:32 600.0 nm 0.854 ABS
Calibration
1 WL Calibration 1 WL Accuracy
2 Set Time 2 WL Reproducibility
3 Lamp Time 3 Bandpass
4 GLP/GMP 4 Noise Level
5 Baseline Stability
6 Baseline Flatness
7 Hardware
0 Summary Report
Select Function: _
The measure condition of GLP/GMP is shown as below:
Check item Process Specification WL Accuracy Wavelength Scan, Range: (660-
650nm) or (490.0-480.0), speed: 100nm/min
+/-0.3nm
WL Reproducibility
Wavelength Scan, Range: (660-650nm), 3 times, speed: 100nm/min
+/-0.1nm
Bandpass Wavelength Scan, Range: (660-650nm), speed:100nm/min
≤ 1.5 nm
Noise Level Time Scan, WL:500.0nm, 5min 0.001ABS Baseline Stability
Time Scan, WL:500.0nm, 3600s 0.001ABS
Baseline Flatness
Wavelength Scan, Range:950.0-200.0, Speed:400nm/min,
+/-0.004ABS
1. <WL Accuracy> After wavelength scanning from 660.0nm to 650.0nm, the system will get the maximum value and the corresponding wavelength position. If the wavelength position is within the range of 656.1±0.3nm, the WL Accuracy item will display <OK>.
2. <WL Reproducibility>
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DB-20 will perform wavelength scan from 660.0 to 650.0nm as in the WL Accuracy test in the first stage. The maximum value of wavelength will be located, then the motor moves from 650.0 to 190.0nm, and then from 190.0 to 660.0nm. In second stage, DB-20 will scan from 660.0-650.0nm again; find another wavelength position at the same time. In the third stage, DB-20 will moves from 650.0-1100.0nm, from 1100.0-660.0nm and perform a wavelength scan the third time and mark the third wavelength peak position. From the three values recorded in the 3 stages, DB-20 will obtain the maximum wavelength value and the minimum wavelength value, taking the mean of the two values i.e. (max-min)/2. If the result is within 0.1nm, the WL Reproducibility item will display <OK>.
3. <Bandpass> DB-20 perform a wavelength scan from 660.0 to 650.0nm as in WL Accuracy test. From the measured data, DB-20 identified the maximum value and the minimum value and taking the mean of the absorbance value i.e. A/2= (Max-Min)/2. At A/2 Abs value, two wavelength values that are close to A/2 can be identified. The difference between these two wavelengths is the value of band pass. If the bandpass value is within 1.5nm, the bandpass item will display <OK>.
x
0
5
1 2 3 4 5 6 7 8
x
½
A
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4. <Noise Level>
After the time scan of 300S at 500.0nm, DB-20 will identify the maximum value and the minimum value of the measured data. The noise level value is the difference between the maximum value and the minimum value (peak to peak). If the noise level value is within the range of 0.001ABS, the item will display <OK>.
5. <Baseline Stability> After the time scans of 3600S at 500.0nm, the peak-to-peak value of the wavelength scan is defined as baseline stability value. If this value is within the range of 0.001ABS, this item will display <OK>.
6. <Baseline Flatness> DB-20 will perform a wavelength scan from 950.0nm to 200.0nm twice. The first time the measured data is recorded as the baseline, and the second group of data is defined as absorbance. The maximum value and the minimum value will be identified in this scan. The result (Max-Min)/2 is defined as baseline flatness. If the value is within the range of (±0.004ABS), the baseline flatness item will display as <OK>. When one function is completed, the system display <OK> or <NG> message as described in the above.
Press <7> key on the keyboard to check hardware. The hardware of the instrument will be listed on the right side on the screen as shown below.
11/12/02 10:52 600.0 nm 0.854 ABS
Calibration
1 WL Calibration Hardware
2 Set Time RAM OK
3 Lamp Time ROM OK
4 GLP/GMP Wavelength Driver OK
D2 Lamp OKWI Lamp OKAD Conversion OK
D2 Lamp Usage (h) 8WI Lamp Usage (h) 8
Select Function: _
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After checking all the above items of GLP/GMP, press <0> to print the measuring results in the GLP/GMP screen measured.
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3.9 Test Menu
Press [7] on the keyboard to enter the Test Menu mode. The Test Menu submenu will be displayed on the LCD as shown below.
11/28/02 12:38 600.0 nm 0.854 ABS
Test Menu
1 Load Test File2 Delete Test 1 P01 UTQ 11
3 Set Autostart 2 123 13
4 145 156 167 178 189 1910 20
Input File No.(0-20)
3.9.1 Retrieving a Test Stored in Inside Memory
You can retrieve a test method that you have stored in Inside memory by following steps: Press <1> on the keyboard to select the Load Test, and a file list will appear on the side of the screen. Select the desired test number using the UP and DOWN arrow key and then press ENTER. The operating parameters for the selected test are initiated automatically and the screen then advances immediately to the START position.
3.9.2 Deleting Test From Inside Memory
Press [2] on the keyboard to select the Delete Test, and a file list will appear on the side of the screen.
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Use the UP and DOWN arrow keys to select the desired file number and then press [ENTER]. Then the test method has been deleted from the inside memory. Press [RETURN] key at any time if you wish to cancel the method-set deletion request. Note: It is a good laboratory practice routinely to remove test method sets
that you no longer use. This ensures that there always available file numbers to allow the instrument to remember your method for you automatically.
3.9.3 Autostart Function
The AUTOSTART function allows you to select the operating parameters for a particular test, so that the test will be initiated automatically at instrument power-up. Press [3] on the keyboard to select AutoStart function.
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Chapter 4 Maintenance
4.1 Self Diagnosis and Automatic Adjustment
The HALO DB-20 spectrophotometer will execute self-diagnosis and automatic adjustment immediately after the power switch is turned on. For details, refer to chapter 3.
4.2 Confirmation of Specifications
4.2.1 Wavelength Accuracy
1. Verify that the instrument is powered up and wait for 30 minutes at least. 2. Select Wavelength Scan from the Main Menu. 3. Set the operating conditions as shown below:
Data Mode E(R) Start WL (nm) 660 Stop WL (nm) 650 Scan Speed (nm/min) 100 UP Scale 100 LO Scale 0 Lamp Change WL (nm) 340.0
WI Lamp ON D2 Lamp ON Response Fast
4. Verify that the Sample and Reference cuvette holders are empty. Then
press 0 to display the measurement screen. 5. Press START to acquire a spectrum. 6. After the measurement, read the peak wavelength. 7. If the wavelength position is within the range of 656.1±0.3nm, it is
normal. If the peak is not in the range then: a) Change the existing scale to provide an easier to read graph and if
the peak is still not within range, perform the WL Calibration in Chapter 3.
b) Once you have performed the wavelength calibration, repeat steps necessary to confirm that the peak is within the specified normal value range.
4-1
4.2.2 Confirmation of Bandwidth
1. Select Wavelength Scan from the Main Menu and then press ENTER. 2. Set the operating conditions as shown below:
Data Mode E(R) Start WL (nm) 660 Stop WL (nm) 650 Scan Speed (nm/min) 100 UP Scale 100 LO Scale 0 Lamp Change WL (nm) 340.0
WI Lamp ON D2 Lamp ON Response Fast
3. Verify that the Sample and Reference cuvette holders are empty. Then press 0 to display the measurement screen.
4. Press START to acquire a spectrum. 5. Select “Print” to record the spectrum on a graphic plotter, and then check
the bandwidth as shown below: a. Measure the peak width in nm at half-height. b. If this value is 1.5nm±0.3nm, then the bandwidth is normal.
Energy
Peak width at half-height
A2
WL
A
0
4.2.3 Confirmation of Baseline Stability
Be sure to warm up the instrument sufficiently (approx. 2 hours), and then proceed as instructed below:
4-2
1. Select Time Scan from the Main menu. 2. Set the operating conditions as follows:
Data Mode ABS
Wavelength (nm) 500 Scan time (s) 3600 UP Scale 0.001 LO Scale -0.001
Lamp Change Mode Auto Lamp Change WL (nm) 340.0
WI Lamp ON D2 Lamp ON Response Medium
3. After setting up the conditions, press 0 to display the measurement screen. 4. Verify that the Sample and Reference cuvette holders are empty. And then
press Auto Zero. 5. Press START to start a measurement. 6. After measurement, check the baseline stability as shown figure below:
Ｄ
3600sec
4.2.4 Confirmation of Baseline Flatness
Be sure to warm up the instrument sufficiently (approx. 2 hours), and then proceed as instructed below: 1. Select Wavelength Scan from the Main menu. 2. Set the operating conditions as follows:
Data Mode ABS Start WL (nm) 950 Stop WL (nm) 200 Scan Speed (nm/min) 400 UP Scale 0.005 LO Scale -0.005
Lamp Change Mode Auto Lamp Change WL (nm) 340.0
WI Lamp ON
4-3
D2 Lamp ON Response Medium
3. After setting up the conditions, press 0 to display the measurement screen. 4. Verify that the Sample and Reference cuvette holders are empty. And then
press Auto Zero. 5. Press START to start a measurement. 6. After measurement, check the baseline stability as shown figure below:
Flatness
200～950nm
4.3 Periodic Inspection
4.3.1 Cleaning of Sample Compartment
The sample compartment is susceptible to contamination the compartment should be cleaned if a sample is spilled inside. Take out the sample compartment base as Fig. 4-1 and clean it.
Guide Pin
4-4
Fig. 4-1 Method to take out the sample compartment base
1. Open the sample compartment door completely. 2. Loosen the fastening screw with a screwdriver and take out the sample
compartment base.
Fig. 4-2
4-5
3. Clean the sample compartment base. 4. After cleaning the base and reinstall it as the Fig.
4.3.2 Cleaning the Sample Compartment Windows
If the transparent window of the sample compartment is contaminated, remove the window plate, and then clean it completely. Be sure to wash your hands thoroughly before cleaning the window, and then wipe off the contaminant with a soft paper or cloth soaked with alcohol and ether solution (mixed in a ratio of 1 to 1).
CAUTION: Pinch both ends of the window plate with your fingers to set it back in the sample compartment. Do not leave fingerprints on the window plate.
Adhesive tape
Black window frame
(Pull it forward with tweezers)
Window frame
Optical axis
Spring
Transparent Window
Fig. 4-4 Removal of Sample Compartment Window Plate
4-6
4.4 Troubleshooting
If there is an abnormality, correct it referring to the following table Symptom Cause Solution 1 Lamp fails to light if power
switch is turned on. a. Fuse is not in place. b. Fuse is blown.
a. Reinsert the fuse. b. Replace the fuse.
2 “656.1nm NG”, appears on the screen during initialization.
Wavelength calibration values have been deleted.
a. Execute CALIBRATION. b. Contact the nearest Dynamica agent if
the error display does not go off even after executing CALIBRATION.
3 D2 LAMP or WI LAMP NG is displayed on the screen during initialization.
Lamp filament has failed Replace the lamp.
4 “PRINTER NOT READY” appears on the LCD screen.
Printer head is abnormal. a. Turn off the power switch, and see if the error display goes off.
b. Contact the nearest Dynamica agent if the error message is displayed again.
5 Error message appears on the bottom of the LCD.
Instrument has been operated erroneously.
Operate the instrument properly referring to the message displayed.
4-7
Chapter 5 Replacement of Parts
5.1 Consumables and Spare Parts
The following parts are consumables. Contact the nearest Dynamica agent before consuming all of them.
WI Lamp (Tungsten Iodide Lamp): P/N: 25-03-0001 D2 Lamp (Deuterium Lamp): P/N 25-02-0013 Main Power Fuse: P/N 13-02-0004
Table 5-1 Guaranteed life of lamp
Guaranteed ti
Guaranteed Period
WI Lamp 1000h 6 months after delivery to customer’s itD2 Lamp 1000h 6 months after delivery to customer’s it
5.2 Part Replacement
5.2.1 Replacement of Light Source Lamp
Cautions on Replacement of Lamp Do not replace the lamp with the power switch on. Note that high
voltage is applied to the lamp. High voltage is discharged while the power is turned off.
Wait for at least 10 minutes after the power switch is turned on. Wear clean gloves for mounting a new lamp. Do not leave fingerprints
on the lamp tube wall. For D2 lamp, care should be taken to prevent leaving fingerprints on the protruded sections.
The following shows how to replace the existing lamp with a new one.
(1) Turn off the power switch. Pull off the power cord. Then, wait for at least 20 minutes until the lamp cools down.
5-1
(2) Remove the fastening screw from the side face of the light source cover, and then remove the cover. (See Fig. 5-1)
Fastening Screw
Fig. 5-1 remove the cover
After open the cover, the source lamp can be seen as fig. 5-2.
D2 lamp
WI lamp
Fig.5-2
(3) Replacement of WI Lamp Loosen the retainer spring, and then pull it out. Push in a new lamp to the extreme end of the lamp holder.
Fig. 5-3 Remove the WI Lamp
5-2
(5) Replacement of D2 Lamp
a) Pull out the D2 Lamp connector.
Fig.5-4
b) Hold the top end of the D2 lamp, and turn it counterclockwise until it is separated from the holder. (See Fig. 5-3)
Fig. 5-5 Remove the D2 Lamp (before turning)
c) Attach a new D2 lamp by reversing the procedure described above. d) Plug in the D2 lamp connector as shown in Fig. 5-5.
5-3
(6) Put back the light source cover. (See Fig. 5-5)
Fig. 5-5
5.2.2 Change Source Lamp Usage Time
After replacement of new WI lamp or D2 lamp, you should clear the lamp usage time. Press <1> or <2> to reset time of WI lamp or D2 lamp to zero from the Calibration submenu, time setting submenu will appear on the right of the screen as shown below.
11/16/02 15:25 600.0 nm 0.854 ABS
Calibration
1 WL Calibration 1 Change New WI Lamp
2 Set Time 2 Change New D2 Lamp
3 Lamp Time WI Lamp Usage (h) 10
4 GLP/GMP D2 Lamp Usage (h) 10
Select Function: _
5-4
If you have any question about the instrument, please contact the producer.
5-5
Notice For the updated contact of Dynamica, applications and other product updated information about HALO DB-20 spectrophotometer. Please visit Dynamica website periodically. Dynamica Website: http://www.dynamica.com.au/
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