Ha See Nullah

8/12/2019 Ha See Nullah

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EFFICACY OF DIFFERENT FUNGICIDES ON FRUIT

ROTS OF CHILLI (ALTERNARIA ALTERNATA.) IN

VITRO AND VIVO CONDITION

SUPERVISOR:

Mr. AKHTAR GULCO-SUPERVISOR

MR. FAISAL ADNAN

MEMBER

MR. AHMED KHAN STUDENT:

HASEENULLAH

DEPARTMENT OF PLANT PATHOLOGY

BALOCHISTAN AGRICULTURE COLLEGE, QUETTA



RATIONALE/PROBLEM STATEMENT

Chilli is grown in four agro-ecological zones of Balochistan

and farmers get high income from chilli growing. However ,

Chilli is susceptible to several diseases particularly fruit rot

of chilli is of great importance in terms of yield and quality

losses. The chemical control of this disease is the easiest

option in farmer’s perspective. So, this study is designed to

evaluate the efficacy of different fungicides against the fruit

rot of Chilli caused by Alternaria alternate.



OBJECTIVES

To isolate, purify and identify the causal organism of

chilli fruit rot (Aternaria alternata ) and prepare its

culture in the laboratory.

To find out the most effective fungicide against chilli

fruit rot caused by Aternaria alternata under in vitro

and vivo condition.



INTRODUCTION

Chilli (Capsicum annuum L.) is considered an important tropical

and subtropical crop on the basis of its high consumption,

nutritional and cash value to farmers and consumers both in

developed and developing countries.

In Pakistan chilli (Capsicum annum L.) is an important

vegetable crop planted over an estimated area of 47.3 thousandhectares with an annual production of 69.5 thousand tones, with

an average yield of 1902 kg/ha.





MATERIAL AND METHODS

Four fungicides i.e. Diathene M45, Topsin, Allete, and Revus will be evaluated against Chilli fruit rot caused by Alternaria

alternata in vitro and vivo condition that will be replicated three

times. In vitro study will be conducted in Plant Pathology

Laboratory Balochistan Agriculture College Quetta using CRD

and in vivo study will be carried out at the experimental field

using RCBD at Directorate of Agriculture Research Plant

Protection, Agriculture Research Institute Sariab Quetta during

2013.



IN VITRO STUDY

Alteranaria alternata culture will be prepared from infected chilli

fruit that will be used for the assessment of fungicides efficacy.

Different concentrations of the tested fungicides with specific

volumes will be added to the proper amounts of PDA medium

before solidification and then poured into sterilized Petri-dishes

(9cm diam.).

All dishes will be inoculated in the centre from the Alteranaria

alternata cultures.

All dishes will be incubated at 30±1°C for 7 days then the number

of colonies of fungus will be counted and the average will be

calculated.



IN VIVO STUDY

T1 T3 T4

T2 T5 T2

T3 T1 T3

T4 T2 T5

T5 T4 T1

T1 = No fungicide spray(control)

T2 = Diathene M45 spray

T3 = Topsin spray

T4 = Allete spray

T5 = Revus spray

R1 R2 R3

7 m

6 m

Treatment = 5 (4 fungicides and one control)

Plot size = 6x7 m = 42 m2

Replication = 3

Statistical design = RCBD

Randomized complete block design Water Channel

LAY OUT



OBSERVATIONS

Number of colonies counted per treatment

In vitro study

In vivo study

Disease severity assessed one week after each spray and theaverage will be calculated

The number of infected fruits/plot

Fruit weight (kg/plot)



STATISTICAL ANALYSIS

The collected data will be subjected to statistical analysis

using computer software STATISTIX 8.1 (Russel and

Eisensmith, 1983). Treatment averages will be compared at

0.05 level of probability using L.S.D. procedure (Fisher,

1948).



IN VITRO RESEARCH WORK IS BEING CONDUCTED IN

THE LABORATORY OF PALNT PATHOLOGY

BALOCHISTAN AGRICULTURE COLLEGE QUETTA



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