Embed Size (px)
Citation preview
GUSTA ME
¿Te gusta la estadística?
An online guide to statistical analysis in microbial ecology
Roman FeldbauerDivision of Computational Systems Biology
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Multivariate statistical analysis in microbial ecology
● Which technique for which data?● Ongoing development of methodology● 'Prevalence rate' of statistical
expertise?
→ Where to find guidance?
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Multivariate statistics for microbial ecologists
● Online resource● Techniques, relevance, debates● Different entry points/approaches
● GUSTA ME webpage:
http://mb3is.megx.net/gustame
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Reference pages● High-level descriptions of techniques● Principles, assumptions, limitations,
statistical and ecological interpretation plus common pitfalls
● Examples: PCA, NMDS, db-RDA, ...
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
Interactive guides:● Suitable methods for given data set● Decrease and conquer strategy● 'Decision tree' based on (relatively)
easy questions● Suggestion of one or several
appropriate techniques
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Social cognitive theoretic approach● Flowcharts: Analytical methodology
of peer-reviewed studies● Learning by imitation
● http://mb3is.megx.net/gustame/ walkthroughs
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Visualization makes high-dimensional data human readable
● Browsing different types of viz● 'Putting the cart before the horse'
approach
● http://mb3is.megx.net/gustame/home/ visualisations
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Multivariate AnalysiS Applications in Microbial Ecology
● Web front-end for several R tools● Point-and-click style, no R knowledge
required● Example data sets provided● Direct links from end-points
(e.g. PCA, NMDS, db-RDA)
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Community involvement● Online forums
– Comments on content
– Method discussion
– Suggest revisions
– Contribute walkthroughs etc.
● Community still needs to grow...
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Student– Given data set, follow a wizard, analysis using
MASAME, publish study with appropriate multivariate statistics
● Principal investigator– Designing a study, browses walkthroughs, adjusts
sampling design and replication strategy
● Reviewer– Needs to check appropriateness of a certain
technique, jumps directly to corresponding end-point, may check key assumptions with MASAME
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
● Current state is a starting point, useful resource for multivariate statistics
● Might become great knowledge base● Some issues
– Community involvement
– Minor inconsistencies
– Web design
GUSTA ME
Motivation
GUSTA ME
End-points
Wizards
Walkthroughs
VIZ libraries
MASAME
Community
Usage ex.
Outlook
Me gusta
Gracias por su atención
