Growth of phage materials

Mod3 Day1

Tuesday Nov 4, 2008

p3

p8]> 20 nm

DNA

p9 p7

p6

900 nm

10^10th phage/ml of seawater

2700 copies

BioMaterials: Let’s start with the biology

p3

p8]> 20 nm

DNA

p9 p7

p6

900 nm

2700 copies

BioMaterials: Let’s start with the biology

1. Infection

2. Amplification

3. Morphogenesis + release of progeny phage

• new M13 phage particles within 10’ post-infection

• 1000 phage/cell within the first hour

BioMaterials: Let’s start with the biology

BioMaterials: Let’s start with the biology

Parts 2 + 3 of your experiment today

Phage purification

• Harvest phage infected cells. Phage in SUPERNATANT!

• Concentrate by precipitation (+PEG/salt)

• Pellet may not be visible…have faith

Plaque assay

• Serially dilute phage in water

• Mix with bacteria

• Add warm “top agar” and plate on LB--> 37° overnight

• Give remaining phage to Luis

BioMaterials: now for materials

Oxidized (purple) Reduced (clear)

Part 1 of your experiment today

Hydrolyze Iridium with citrate at 90° 4 hrs

Generates Ir colloidal particles

Next time will oxidize to form IrOx colloidal particles which

can react with phage p8

You must wear safety glasses, gloves, lab coat to do this part

of today’s experiment

FNT

1. Describe display technology

2. Choose research proposal (5 possible topics)

or collaborative art/science option