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Green Synthesis of…… General Introduction
1
Green Synthesis of Bioactive Phenolics Employing Ionic Liquids and
Microwave Assisted Approaches
“Only when I saw the earth from space, in all its ineffable beauty and fragility, did I realize that
humankind's most urgent task is to cherish and preserve it for future
generations”................................................. Sigmund Jahn, German Cosmonaut
1.1 Introduction:
Nature is a phenomenal repository of an amazing array of structurally and functionally
diverse organic compounds which are useful as drugs, fine chemicals, fragrances, flavors
and biologically active dietary constituents. [Treben (1986); Harborne and Dey (1989);
Albert (1996); Clardy and Walsh (2004)]. For example, compounds like artemisinin,
podophyllotoxin, atropine (Figure 1) etc obtained from different plant parts are reported to
possess various bioactivities such as antimalarial, anticancer and anticholinergic activities.
Among the various natural products, phenolic compounds or more specifically ‘phenolics’
are one of the most ubiquitous groups possessing plethora of biological activities [Harborne
and Dey (1989); Chadwick and Marsh (1990); Ahmad et al. (2006); Crozier et al. (2009)].
For instance, various phenolics like resveratrol (trans-3,4',5-trihydroxystilbene) and DMU-
212 (trans-3,4,4',5-tetramethoxystilbene) have shown potential as therapeutic agents against
various cancer cell lines [Shen et al. (2009)]. Similarly, α-asarone (trans-2,4,5-trimethoxy-
1-phenylpropene) has been recognized as a cardioprotective agent due to its hypolipidemic
activity [Popławski et al. (2000)]. Recently, phenolic derivatives (e.g. chalcones) have been
O
O
H
H
H
O
O
O
Artemisinin(Antimalarial)
OO
O
OHH
H O
H3COOCH3
OCH3
Podophyllotoxin(Anticancer)
Figure 1
O
O
OHN
Atropine(Anticholinergic)
Green Synthesis of…… General Introduction
2
reported as appropriate partner in artemisinin based combination therapies (ACTs) against
malaria [Bhattacharya et al. (2009)].
Owing to the immense importance of phenolics, interest in accessing these molecules has
further intensified the research [Ahmad et al. (2006); Sinha et al. (2010)]. However, these
compounds are often present in only feeble concentration in their natural sources.
Consequently, development of newer methodologies for their synthesis or chemical
modification into various bioactive molecules is gaining ample momentum. However, the
deleterious environmental impact of some of the current chemical practices [Gartner et al.
(2003)] such as use of toxic reagents/solvents, multistep reactions, wastage of energy etc for
the synthesis of above compounds has shifted the attention of scientific community towards
“Sustainable or Green Chemistry” [Anastas and Warner (1998); Li and Trost (2008)]. The
present study aimed to address some of the above challenges using ionic liquids (ILs) and
microwave (MW) as tools of green chemistry by focusing on two important classes of
phenolic compounds viz phenylethanoids and phenylpropanoids.
1.2 Brief description and significance of phenolics:
1.2.1 Phenolics:
The term, phenolics [Harborne and Dey (1989); Harborne et al. (1999)] refers to the
compounds having one or more hydroxyl group attached to the aromatic ring. Phenolics are
widely distributed in the plant kingdom ranging from simple phenol to complex compounds
known as polyphenols. In general, according to the number of carbon atoms in conjunction
with the basic skeleton, phenolics are conveniently classified into various sub-classes like
phenols (C6), phenolic acids (C6-C1), styrenes (C6-C2), cinnamic acids (C6-C3),
naphthaquinones (C6-C4), xanthones (C6-C1-C6), stilbenes (C6-C2-C6), flavonoids (C6-C3-
C6), lignans (C6-C3)2 and lignins (C6-C3)n etc (Table 1) [Harborne et al. (1999)]. In addition,
methylation of hydroxyl functional group or substitution at the phenyl ring further enriches
the phytochemical diversity.
It is evident from Table 1 that phenolic compounds may also bear alkyl side chains to their
core aromatic structure. Further, the functional groups such as -COOH, -CHO, -C=C,
halogen and NO2 etc may be attached either to phenyl ring or to the alkyl side chain.
Green Synthesis of…… General Introduction
3
OH
COOH
HO
O
O
COOH
O
O
O
O
O
O
O
O
O
O
OOH
Carboskeleton Class Basic Structure Examples
C6
C6-C1
C6-C2
C6-C3
C6-C4
C6-C1-C6
C6-C2-C6
C6-C3-C6
Simple phenols
Phenolic acids
Styrenes
Acetophenones
Phenylpropenes
Propiophenones
Cinnamic acids
Naphthaquinones
Xanthones
Stilbenes
Chalcones
Flavones
Anthraquinones
Catechol, Resorcinol
Gallic acid
4-Vinylguaiacol, Canolol
Eugenol, Asarone
Juglone, Plumbagin
Mangostin, Mangiferin
Resveratrol, Combretastatin
Emodin
Lichochalcone A
Sinensetin
Flavanols Catechin
Ferulic acid, Caffeic acid
Table 1. Classification of phenolic compounds along with some examples
Lignans, neolignans(C6-C3)2
(C6-C3)n Lignins Highly crosslinked aromaticpolymer
Pinoresinol, Eusiderin
CHO
HOPhenolic aldehydes
Gallacetophenone
Isoacoramone
Anisaldehyde, Vanillin
Green Synthesis of…… General Introduction
4
In fact, a number of abundantly available bioactive phenolics possessing two to three carbon
side chain are found in nature and have been characterized as phenylethanoids and
phenylpropanoids respectively [Dixon and Paiva (1995); Harborne et al. (1999)]. These
compounds are biosynthetically produced in plants either by the shikimate pathway (which
starts from carbohydrates) or the polyketide pathway (which starts from acetyl and malonyl
coenzyme A) [Dewick (1994)].
A brief description of some of the phenolics (phenylethanoids and phenylpropanoids) taken
up in the present study is given below:
1.2.1.1 Phenylethanoids:
The term “phenylethanoids” represents the organic compounds having C6-C2 skeleton
[Harborne et al. (1999)]. These constitute a large number of compounds such as
phenylethenes (styrenes, stilbenes), phenylethanols, phenyl acetic acids etc (Figure 2), out
of which this study will be mainly focused on synthesis of substituted styrenes and
stilbenes.
R
R'
OH
COOH
RRR
Figure 2. Examples of some phenylethanoids
1.2.1.1.1 Styrenes:
Styrenes, also known as vinyl benzenes, constitute one of the simplest classes of
phenylethanoids.
Significance of styrenes:
As Bioactive and Flavoring agents
Styrenes (Figure 3) are obtained from various natural sources and have immense importance
in flavor and pharmaceutical
industries [Crouzet et al. (1997);
Lasekan et al. (2001)]. For
example, 4-hydroxy-3-
methoxystyrene (4-
vinylguaiacol, Figure 3), is
isolated from a variety of plants
such as Hibiscus esculentus (okra), Feijoa sellowiana (feijoa fruit) etc, which is a FEMA
OHOCH3
OH OHOCH3
Figure 3. Examples of some natural styrenes
4-Vinylguaiacol
H3CO
4-Vinylphenol Canolol
Green Synthesis of…… General Introduction
5
GRAS (Flavour and Extract Manufacturers Association Generally Regarded As Safe)
approved compound [Crouzet et al. (1997)]. On the other hand, 4-hydroxy-3,5-
dimethoxyphenylethene (canolol, Figure 3) is a constituent of rapeseed oil and is reported to
possess potent antioxidant and anticancer activities [(Kuwahara et al. (2004)].
Styrenes as synthons in organic chemistry
One of the most significant applications of styrenes in organic synthesis lies in their use for
the production of fine chemicals through transition metal catalysis. For instance, the
classical Heck reaction which is a Pd-catalyzed cross coupling reaction for the formation of
C-C bond involves styrenes as one of its coupling partner (Scheme 1) [Alonso et al. (2005)].
This transformation is a prominent route towards synthesis of biologically active stilbenes
such as resveratrol and pterostilbene etc.
+X
Pd(OAc)2, PPh3
R R' R
R'
X = Br, I, Cl, OTf etc.R = R' = H, CH3, OCH3 etc.
Et3N, DMF
Scheme 1
Similarly, styrenes via epoxidation and hydroformylation approaches can be converted into
synthetically important compounds such as -arylaldehydes [Kim and Alper (2005);
Sharma (2010)]. In addition, styrenes also act as precursors for the synthesis of various
polymers like styrene-butadiene rubber and styrene-divinylbenzene etc [Atsushi et al.
(1998)].
1.2.1.1.2 Stilbenoids:
Stilbene, (also known as biphenylethene), can be considered
to arise from a core C6-C2 skeleton of styrene by replacement
of H atom from side chain with a C6H5 moiety (Figure 4).
Stilbenes and their derivatives including dihydrostilbenes are
commonly known as stilbenoids and are widely present in
nature [Jang et al. (1997); Xiao et al. (2008)]. As such these are known as phytoalexins, a
class of defense compounds synthesized by plants in response to pathogens and abiotic
stress.
Figure 4. Stilbene
Green Synthesis of…… General Introduction
6
Significance of stilbenoids:
As bioactive agents
Stilbenes are known to have a number of biological activities. For example, various
stilbenes such as resveratrol, pterostilbene, DMU-212 and combretastatin A-4 (Figure 5)
have been explored as chemotherapeutic agents against various cancer cell lines [Rimando
et al. (1994); Jang et al. (1997); Sale et al. (2005); Delmas et al. (2006)]. The biological
potential of various stilbenes has further been discussed in details in chapter 4.
OH
HO
OH
Resveratrol Pterostilbene
Figure 5. Some anticancer stilbenoids
DMU-212
OCH3
H3CO
OCH3
H3CO
H3CO
OCH3H3CO
OCH3
OHOCH3
H3CO
OH
Combretastatin A4
Stilbenes as precursors
Aslam et al. reported the synthesis of cicerfuran, an antifungal benzofuran, by epoxidation
and cyclization of corresponding 2-hydroxy-substituted stilbene (Scheme 2) [Aslam et al.
(2006)].
O
O
TBDMSO OTBDMS
OMe
O
O
HO OH
OMeO
O
O OMe
O
m-CPBA
OH
Scheme 2
DCM CHCl3PTS A
Similarly, biological active moracins, norlignans and phenanthrene alkaloids have been
synthesized from corresponding stilbenes [Kumar (2007)]. In addition, use of
polyaromatic/conjugated stilbenes in organic light emitting diodes, laser dyes,
photochemically crosslinked polymers and nonlinear optical devices etc is a subject of
prime interest [Wei and Chen (2007); Kalanoor et al. (2009)].
1.2.1.2 Phenylpropanoids:
The phenylpropanoids are the compounds possessing one or more C6-C3 skeleton [Dixon
and Paiva (1995); Vogt (2010)]. Thus, this is a diverse family comprising of compounds
such as phenylpropenes, propiophenones, chalcones, cinnamaldehydes, coumarins etc, out
of which this study is mainly focused with phenylpropenes, propiophenones and chalcones.
Green Synthesis of…… General Introduction
7
1.2.1.2.1 Phenylpropenes:
Phenylpropenes are compounds having a phenyl ring and a three carbon side chain with at
least one double bond (Figure 6). Various phenylpropenes including anethole,
methylisoeugenol, α-asarone and safrole etc are produced by a majority of plants for various
roles such as a part of chemical defense mechanisms and for biosynthesis of other plant
derivatives like flavonols, lignans and lignins etc [Risch and Chi-Tang (1997); Vogt
(2010)].
OCH 3 OCH3
H3CO
OCH 3OCH 3
H3CO
OCH 3H3CO
OCH3
Anethole Methylisoeugenol Asarone -Asarone
Figure 6. Examples of some natural phenylpropenes
Significance of phenylpropenes
Biological importance
Phenylpropenes have been found to possess hypolipidemic, antiatherogenic, anti-
inflammatory, anticholeretic, antifungal and antiplatelet activities [Risch and Chi-Tang
(1997); Harborne et al. (1999); Popławski et al. (2000)]. However, the biological activity of
phenylpropenes obtained from plants is highly influenced by the variation in their isomeric
forms (, β and γ). For example, α-asarone (trans-2,4,5-trimethoxy-1-phenylpropene, Figure
6,) has been recognized as a cardioprotective agent due to its hypolipidemic activity
[Popławski et al. (2000)], whereas, β-asarone (cis-2,4,5-trimethoxy-1-phenylpropene,
Figure 6) is toxic in nature. Similarly, trans-anethole stimulates hepatic regeneration in rats
and also shows spasmolytic activity.
Synthons in organic chemistry
One of the major synthetic utilities of phenylpropenes lies in their use as abundantly
available hydrocarbon feedstocks for synthesis of value added compounds. The peculiar
presence of benzylic double bond allows their easy functionalization into a wide array of
biologically and industrially important products such as cinnamaldehydes, neolignans and
benzaldehydes etc [Sinha et al. (2002); Leite et al. (2004); Freire et al. (2005); Joshi et al.
(2005, 2006)]. Recently, Sharma et al. developed a green route for the conversion of various
phenylpropenes into synthetically important -arylaldehydes [Sharma et al. (2009)].
Green Synthesis of…… General Introduction
8
1.2.1.2.2 Propiophenones:
Propiophenones are a class of phenolic compounds containing a ketonic group in the propyl
side chain. A large number of substituted propiophenones containing hydroxy,
methylenedioxy and methoxy groups at the aromatic ring have been found in nature
[Harborne et al. (1999)]. Some of the propiophenones are listed in Figure 7.
OMe
MeO
OM eMeOO
O
OO O
O
1-propiophenone 4-methoxy-propiophenone
3,4-methylenedioxy-propiophenone
2,4,5-trimethoxy-propiophenone
Figure 7. Examples of some propiophenones
Propiophenones possess a wide range of biological activities such as anti-PAF, H3-receptor
antagonist, antifungal and hypolipidemic activities [Zacchino et al. (1999)]. For instance,
isoacoramone (2,4,5-trimethoxypropiophenone) found in Piper marginatum (Piperaceae)
and Acorus tararinowii (Araceae) is a potential hypolipidemic compound.
1.2.1.2.3 Chalcones:
Chalcones or 1,3-diaryl-2-propene-1-ones are prominent
plant secondary metabolites and represent another
important subclass of phenolics [Patil et al. (2009)]. The
two aryl rings of chalcone are linked by a three carbon
α,β-unsaturated carbonyl system (Figure 8). Substitution
on both rings (A & B) of chalcone can be easily achieved by their synthesis through
Claisen-Schmidt condensation of substituted benzaldehyde with acetophenones using acid
or base catalysis [Nowakowska (2007); Patil et al. (2009)].
1.2.1.2.3.1 Chalcones as bioactive agents:
Chalcones have been found to possess a wide range of biological properties such as
antioxidant, antileishmanial, antimalarial, pesticidal, antitumor and antibacterial activity
[Modzelewska et al. (2006); Nowakowska (2007)]. Despite a large number of biological
activities, the present work is mainly focused on to see the effect of various substituents on
ring A as well as on ring B of chalcone for antimalarial and pesticidal activity and thereafter
to find out structure-activity relationship.
o
A B
Figure 8. Chalcone
Green Synthesis of…… General Introduction
9
1.2.1.2.3.2 Chalcones as antimalarial agents:
The antimalarial activity of chalcones was first noted when licochalcone A (1,1,-dimethyl
allylated natural product, Figure 9), an oxygenated chalcone isolated from Chinese liquorice
roots, was reported to exhibit potent in vivo and in vitro antimalarial activity against both
chloroquine-susceptible and chloroquine-resistant P. falciparum strain [Chen et al. (1997)].
Similarly, crotaorixin (Figure 9) isolated from the aerial parts of the Crotalaria and
diprenylated chalcone, medicagenin (Figure 9) isolated from the roots of Crotalaria
medicagenia have been found to show good antimalarial activity [Narender et al. (2005);
Nowakowska (2007)].
HO
OH
OH
OCH 3
O
HO
OH
OH
O
OH
OCH 3
HO
O
Crotaorixin MedicageninLicochalcone A
Figure 9. Some natural antimalarial chalcones
Also, various chalcones and their derivatives have been investigated for their pesticidal
activity against Culex quinquefasciatus [Das et al. (2010)] and Periplaneta americana etc
[Gautam and Chourasia (2010)].
1.3 Current challenges in context of phenolics compounds:
Owing to such a huge importance of phenolic compounds, various protocols are available in
the literature for their synthesis or chemical modification into other bioactive molecules.
Although some of these methodologies are beneficial in their own right, however, majority
of the available methods continue to be afflicted with challenges like use of toxic
reagents/solvents, multi-step reaction, delicate reaction conditions, prolonged reaction time
besides use of energy intensive processes. In particular, excessive use of the halogenated
solvents in organic synthesis is associated with grave perils like ozone layer depletion and
environment change [Gartner et al. (2003)]. Moreover, these solvents have been proven to
be human carcinogen. Hence, there is a growing concern in the society towards sustainable
development using environmental benign processes. In the above context, the concept of
Green Chemistry assumes paramount importance as it seeks to provide a platform for
sustainable chemical practices.
Green Synthesis of…… General Introduction
10
1.4 Green Chemistry:
Green chemistry is the design of chemical products and processes aimed at eliminating the
use and generation of hazardous substances. Chemical products can be manufactured using
a wide variety of synthetic routes. Green chemistry enhances the safety of a process by
employing inherently safer substances throughout the process designing including the
selection of substrate, catalysts, solvents and designing the final product so that it too is
innocuous. Green chemistry also promotes the use of agricultural based renewable starting
materials instead of depleting petroleum based feedstock. The essential features of the
concept of Green Chemistry have been enunciated in the form of a set of twelve principles
by Prof. Paul Anastas of the United States Environmental Protection Agency and Prof. John
C. Warner [Anastas and Werner (1998)] as mentioned below:
1.4.1 Principles of Green Chemistry:
(1) Prevention: It is better to prevent waste than to treat or clean up waste after it has been
created.
(2) Atom economy: Synthetic methods should be designed to maximize the incorporation
of all materials used in the process into the final product.
(3) Less hazardous chemical syntheses: Wherever practicable, synthetic methods should
be designed to use and generate substances that possess little or no toxicity to human health
and the environment.
(4) Designing safer chemicals: Chemical products should be designed to effect their
desired function while minimizing their toxicity.
(5) Safer solvents and auxiliaries: The use of auxiliary substances (e.g., solvents,
separation agents, etc) should be made unnecessary wherever possible and innocuous when
used.
(6) Design for energy efficiency: Energy requirements of chemical processes should be
recognized for their environmental and economic impacts and should be minimized. If
possible, synthetic methods should be conducted at ambient temperature and pressure.
(7) Use of renewable feedstocks: A raw material or feedstock should be renewable rather
than depleting whenever technically and economically practicable.
(8) Reduce derivatives: Unnecessary derivatization (use of blocking groups, protection/
deprotection, temporary modification of physical/chemical processes) should be minimized
or avoided if possible, because such steps require additional reagents and can generate
waste.
Green Synthesis of…… General Introduction
11
(9) Catalysis: Catalytic reagents (as selective as possible) are superior to stoichiometric
reagents.
(10) Design for degradation: Chemical products should be designed so that at the end of
their function they break down into innocuous degradation products and do not persist in the
environment.
(11) Real-time analysis for pollution prevention: Analytical methodologies need to be
further developed to allow for real-time, in-process monitoring and control prior to the
formation of hazardous substances.
(12) Inherently safer chemistry for accident prevention: Substances and the form of a
substance used in a chemical process should be chosen to minimize the potential for
chemical accidents, including releases, explosions and fires.
The above principles can be incorporated into any chemical process by using some enabling
tools. However it is not possible to achieve these entire goals simultaneously in a chemical
process. In this context, the present studies especially involve the application of the
following tools of Green Chemistry:
Tandem reactions
Use of renewable starting materials
Use of environmentally benign solvents: Ionic liquids
Energy conservation: Microwave-assisted organic synthesis
1.4.2 Tandem reactions:
One of the classical strategies for the synthesis of organic compounds utilizes linear and
stepwise processes involving isolation and purification of each intermediate and often lead to
reduced yields. The situation becomes even more problematic when the intermediates are
unstable and therefore difficult to isolate. In this context, tandem (or domino) synthesis
allows the formation of several bonds in one operation without isolating the intermediates or
changing the reaction conditions thus saving lot of solvent, energy and time [Parsons et al.
(1996); Tietze (1996)]. The term tandem, domino, cascade, and sequential are often used
indistinguishably from one another [Nicolaou et al. (2006)], although some efforts for the
classification of above reaction terminologies have been made in literature. As defined by
Tietze [Tietze (1996)], “A tandem reaction is a process involving two or more bond-forming
transformations which take place under the same reaction conditions without adding
Green Synthesis of…… General Introduction
12
additional reagents and catalysts, and in which the subsequent reactions result as a
consequence of the functionality formed in the previous step. Tandem reactions not only
increase the yield of a synthetic route but also provide chemo- and regioselective control in
an efficient and atom-economical manner. For instance, Angelin et al. reported a tandem
reaction for the synthesis of 3-substituted isoindolinones using 2-cyanobenzaldehyde and
primary nitroalkanes [Angelin et al. (2010)]. The reaction proceeds via formation of
nitroaldol (Henry reaction), followed by a subsequent cyclization and rearrangement as
mentioned in Scheme 3.
CN
H
OR NO2
Et3NNH
O
NO2
R
OHN
NO2
R
Rearrangement
Scheme 3
R = CH3, C2H5, Ph, etc.
However, designing of a new tandem processes is highly dependent on the judicious
selection of catalyst and reaction conditions so that any interference or cross contamination
between the reagents in concurrent steps could be avoided [Baidossi et al. (2005); Kumar et
al. (2010)].
1.4.3 Use of renewable starting materials:
Ideally, a chemical synthesis should utilize readily available or more preferably renewable
source of starting materials [Bjørsvik and Liguori (2002); Meier et al. (2007)] instead of
depleting petroleum based feedstock. In this context, the use of abundantly available plant
derived raw materials as synthons is highly advantageous as these often provide convenient
templates to efficiently build up a wide array of value added compounds.
For instance, Acorus calamus (family: Araceae) is a rich source of various phenylpropenes
with -asarone as its major component (upto 96%). Recently, -asarone (cis isomer) of
Acorus calamus oil is proved to be toxic and carcinogenic whereas its trans isomer i.e –
asarone is an active hypolipidemic agent. The above problem has highly affected the
economic prospects of this crop for industries as well as farmers. In this context, Sinha and
co-workers have developed various green methodologies utilizing this inexpensive starting
materials for the synthesis of a wide range of commercially important bioactive phenolics
including hypolipidemic active -asarone (Figure 10) [Sinha et al. (2002); Joshi et al.
(2005); Sharma et al. (2009); Sharma et al. (2010)].
Green Synthesis of…… General Introduction
13
Similarly, dihydrotagetone (2,6-dimethyl-7-octen-4-one) is an abundantly available
terpenoid isolated from several plants including Tagetes minuta [Baser and Malyer (1996)].
This compound has proved to be an efficient substrate for semi-synthesis of 5-isobutyl-3-
methyl-4,5-dihydro-2(3H)-furanone which is an analogue of a commercially important
naturally occurring flavoring agent whisky lactone [Sinha et al. (2007a)]. In addition, ferulic
acid rich fractions isolated from the maize, corn or rice bran are used as renewable
feedstock for the synthesis of flavoring compounds such as vinyl phenols, vanillin etc
[Lesage-Meessen et al. (1999); Gioia et al. (2009)].
1.4.4 Selection of solvent in a chemical reaction:
Solvents are one of the auxiliary materials used in chemical synthesis to facilitate mass
transfer. However, excessive use of organic solvents like benzene, toluene, dichloromethane
and chloroform etc for various reactions is a major concern in today’s chemical processing
industries due to their deleterious impact on environment and human health. In view of the
above concern there is a need to minimize the amount of solvents during a chemical
synthesis or to find an alternate for halogenated toxic solvents which is one of the key areas
of green chemistry. Some of the strategies include reactions on solid support (heterogeneous
catalysis), use of water or supercritical fluids as solvents etc. Recently, ILs have attracted
much attention [Anastas and Warner (1998); Earle et al. (2000)] as ecofriendly solvents for
chemical synthesis.
OMe
MeO
OMe
CHO
OMe
MeO
OMe
CHO
OMe
MeO
OMe
OMe
MeO
OMe
OMe
OMe
OMe
OMe
MeO
OMe
OMe
MeO
OMe
O
Figure 10. Synthesis of value added products from natural-asarone of Acorus calamus oil
OMe
MeO
OMe
Green Synthesis of…… General Introduction
14
1.4.4.1 Ionic liquids:
The term ILs generally refers to those salts, which have melting points below 100oC
[Welton (1999); Plechkova and Seddon (2008)]. Particularly, the salts that melt at room
temperature are known as “room temperature ionic liquids” (RTILs). This temperature limit
does not have any physical or chemical significance and is just an indicator to distinguish
the ILs from high-temperature molten salts. ILs generally consists of relatively large organic
cation such as imidazolium or pyridinium based cation, whereas anion can be
organic/inorganic such as Cl-, Br-, BF4-, PF6
-, NO3-, [AcO]-, [N(CF3SO2)2]-, [CF3CO2]-,
[CF3SO3]- and [SCN]- etc [Chowdhury et al. (2007); Plechkova and Seddon (2008); Palou
(2010)]. Some of the commonly used cations and anions used for the synthesis of ILs are
depicted in Figure 11.
N NR1H3C N
R
R1
N
R3
R2
R4
R1
P
R3
R2
R4
1-alkyl-3-methyl-imidazolium
[Cnmim]+
1-alkyl-pyridinium
[CnPy]+
NR1 R2
1,1-dialkyl-pyrrolidinium
[CmCnpyr]+
tetraalkyl-ammonium
[Nijkl]+
tetraalkyl-phosphonium
[Pijkl]+
Some common cations:
Some common anions:Water-immiscible Water-miscible
PF6-
[(CF3SO2)2N]-
BF4-
[CF3SO3]-
[CH3COO]-, [CF3COO]-
Br-, Cl-, I-, [NO3]-, [Al2Cl7]-
Figure 11. Examples of some commonly used cations and anions of ionic liquids
+ + ++
Where, n = number of carbon atoms in the linear alkyl chainindicies i, j, k and l indicate the length of the corresponding linear alkyl chains
The field of ILs began in 1914 when Pual Waldon studied the electrical conductivity of
organic ammonium salts which have been found to melt nearly upto 100°C. These
anhydrous salts such as ethylammonium nitrate [EtNH3][NO3] were synthesized by the
neutralization reaction of ethylamine with nitric acid. However, the earliest application of
ILs dates back to 1948 when chloroaluminate salts were used as bath solution for
electroplating aluminium [Wasserscheid and Keim (2000)].
Green Synthesis of…… General Introduction
15
1.4.4.1.1 Classification of ionic liquids according to their acidity or basicity:
The design and choice of an IL in a particular reaction is greatly determined by various
properties such as water miscibility, viscosity, density etc. On the other side, less attention
has been paid to assess the role of acidity or basicity of ions of ILs. In the year 2006,
MacFarlane and co-workers demonstrated that the nature of the IL (as acidic, neutral or
basic) significantly affects the catalytic properties and applications in various organic
transformations [MacFarlane et al. (2006)]. Some of the cations and anions of ILs
categorized according to their Lewis acid/base properties are given in Figure 12
[MacFarlane et al. (2006); Hakala (2009)].
Acidic HN N R
N N SO3H
N N COOHN N
SCl
O
R
RR
(Protonated cation)
AlCl4 FeCl4
(Lewis acidic)
HSO4 H2PO4
(Bronsted amphoteric)
Neutral
N NR3R1
R2
N
R2
R1 R3
R4
S
R1
R3 R2
NR1
PF6 BF4 NO3
Br Cl R-SO3
S
O
O
S
O
O
CF3F3CN
BasicN
NR
(1-alkyl-4-aza-1-azoniabicyclo-[2.2.2]octane
N
NN
Cations Anions
H3C O
O HO
O
O
Type
R2
Figure 12. Structures and acid/base properties of some common cations and anions of ILs
1.4.4.1.2 Properties of ionic liquids:
ILs as reaction media have several interesting properties such as:
Negligible vapor pressure: ILs have negligible vapor pressure and as a result they can be
used as environmental friendly substitutes for volatile halogenated organic solvents.
Green Synthesis of…… General Introduction
16
Recyclability: ILs have been proven to be good solvents for metal catalyzed reactions due to
recyclability of expensive metal complexes besides easy work up of reaction products.
Catalytic properties: ILs can act as a catalyst as well as solvent besides their applications in
asymmetric synthesis and biotransformation studies.
High thermal stability: Most of the ILs have been found to be stable at temperature higher
than 300°C. In addition, ILs are non-flammable and can be handled very easily.
Wide liquid range: ILs have the ability to dissolve a wide variety of organic, inorganic and
organometallic compounds.
Designer property: Most interesting property of ILs is their tunable nature i.e. various
properties of ILs such as miscibility with water and other solvents, dissolving ability,
polarity, viscosity, density etc can be tuned by an appropriate choice of the anion and the
cation, hence these are also called as designer or tunable solvents.
1.4.4.1.3 Preparation and applications of ionic liquids in organic synthesis:
The basic concept for the synthesis of imidazolium based IL containing halide anion (e.g.
Cl, Br, I) involves the quaternization reaction of 1-alkylimidazole with corresponding alkyl
halide (Scheme 4) [Laus et al. (2005); Polshettiwar and Varma (2008)]. The halide anion of
above IL can further be converted into various other anions (such as BF4, PF6 etc) through
metathesis reaction with (i) metal salts or (ii) strong Bronsted acids. On the other hand,
addition of Lewis acid to above halide IL results in the formation of a complex anion e.g.
chloroaluminate ILs. The general procedure for the preparation of 1,3-dialkylimidazolium
based ILs is depicted in Scheme 4.
N NR2R1
X
N NR1
R2X
NaBF 4
NaXN N
R2R1
BF4
N NR2R1
AlCl4
AlCl3
NaPF 6N NR2R1
PF6
NaX
Scheme 4. General procedure for the synthesis of imidazolium based ionic liquids
X = Cl,Br or IR1 = R2 = CnH2n+1
Green Synthesis of…… General Introduction
17
For halide free synthesis of ILs, alkyl carbonates in place of alkyl halides have also been
used as alkylating agents.
In synthetic organic chemistry, ILs have successfully been explored for various reactions
such as Diels-Alder, Mannich, Knoevenagel, Heck, Aldol condensation, Friedal-Craft
reaction etc besides their applications in synthesis of pharmaceutical intermediates
[Malhotra (2007); Jain et al. (2005); Kumar et al. (2007a)]. For instance, Earle et al.
achieved the synthesis of NSAID, Pravadoline in ionic liquid [bmim]PF6 without the need
of any Lewis acid [Earle et al. (2000)] (Scheme 5). The use of IL not only eliminates the
waste disposal problems associated with conventional Friedal-Craft reaction but can also be
recycled.
NH
CH 3N
CH3
N
O
NCH3
N
O
O OCH3
NOCl
HC l.
+Base
[bmim]PF6 [bmim]PF6
Cl
O
H3CO
Scheme 5
ILs have also been used for the production of sustainable and alternative sources of energy
like 5-(hydroxymethyl) furfural (HMF), an intermediate for biofuel industry. Yong et al.
utilized ionic liquid [bmim]Cl along with N-heterocyclic carbene complex of chromium
(NHC-Cr) for the dehydration of glucose and fructose into HMF (Scheme 6) [Yong et al.
(2008)].
OHOHO
OHOH
OH
OHO
HMF
Glucose
OHO
OH
OHHOOHNHC-Cr
Imidazolium saltsbasemetal chloride
DMF 80 °C
[bmim]Cl80°C
(-3H2O)
[bmim]Cl100°C
(-3H2O)
O
Scheme 6
Fructose
Since various permutation of cation and anion can lead to at least a million binary and 1018
ternary ILs whereas only approx. 600 molecular solvents are in use today [Plechkova and
Green Synthesis of…… General Introduction
18
Seddon (2008)]. Being such a huge diversity, there exist numerous opportunities for the
synthesis of various novel ILs to explore them for a particular task.
1.4.4.1.4 Task-specific ionic liquids or functionalized ionic liquids:
The terms ‘task-specific ionic liquids (TSILs)’ or ‘functionalized ionic liquids’ are used for
those ILs which contain additional functional groups as a part either at anion or at the cation
[Lee (2006); Giernoth (2010)]. Thus, ILs possessing various functional groups such as
amine, nitrile, ether, alcohols, acid, urea etc have shown great promises as a reagent/catalyst
besides their role as a solvent. Some of the examples and applications of imidazolium salts
containing functionality at alkyl side chain are shown below (Figure 13) [Lee (2006)].
XN N
R FunctionalGroup
-NH2
-OH, -OR
-SH-PPh2
-Si(OR)3
-Urea & thiourea
-Metal complex etc.
Catalysis
Organic synthesis
Extraction and dissolution
Nanoparticles
Conductive materials
Figure 13
For instance, Wang et al. designed and synthesized a TSIL which acts as a base, ligand and
recyclable reaction media for Heck reaction (Figure 14) [Wang et al. (2009)]. Similarly,
immobilised metal ion containing ILs have been reported as reusable catalytic systems for
various organic reactions such as Kharasch reaction [Sasaki et al. (2005)]. Recently, the
area of TSILs has been expanded through the development of chiral ionic liquids (Figure
14) [Luo et al. (2006)]. Such ILs are usually designed from precursors such as chiral
amines, alcohols or amino acids etc.
(n-C4H9)3NN
OH
OH
Br
Organic Base
Effectiveanion
Figure 14. Examples of some task-specific ionic liquids
N,O-Ligand
(For Heck reaction)
O OSi
N
N
O OSi
N
N
Cu
SiO 2 SiO 2
Cl
ClCl
Cl
H3CO OCH 3
(For Kharasch reaction)
NH
N
N
Bu
BF4-
(Asymmetricorganocatalysts forMichael addition)
Green Synthesis of…… General Introduction
19
In addition, various TSILs have found applications as lubricant, magnetic materials besides
their ability for the absorption of gases such as CO2, SO2 etc [Giernoth (2010)]. Moreover,
ILs having high nitrogen content have been described as “energetic materials” finding
applications in civil as well as for military purposes [Smiglak et al. (2007)].
The first successful example of an industrial process using IL technology was the BASILTM
(Biphasic Acid Scavenging utilising Ionic Liquids) process started in 2002 [Plechkova and
Seddon (2008)]. The use of IL in BASIL process increased the productivity of their
alkoxyphenylphosphine (photoinitiator precursor) formation by a factor of 80000 compared
with the conventional process [Rogers and Seddon (2003)]. Although, research in the field
of IL is booming day by day, however there are certain limitations pertaining to ILs which
need to be resolved.
1.4.4.1.5 Some limitations of ionic liquids:
ILs possessing negligible vapor pressure are rarely flammable or explosive meaning that
they do not contribute to air pollution. However their high solubility in water may pose
environmental risks to aquatic ecosystem [Cho et al. (2008)]. Recent research on ILs in
aquatic system have shown them to be toxic against various organisms including Vibrio
fischeri [Docherty and Kulpa Jr. (2005)], Daphnia magna [Bernot et al. (2005)], algae
[Latala et al. (2005)], Escherichia coli [Lee et al. (2005)] and Danio rerio [Pretti et al.
(2006)] etc.
Another disadvantage is that ILs possessing BF4 or PF6 as counter anions sometimes
produce hydrofluoric acid (HF) as a byproduct due to hydrolysis. The use of such ILs could
be a problem for industry because HF is highly corrosive and dissolves all inorganic metal
and semimetal oxides. However, in positive sense, such ILs can be used to deliver this
corrosive acid (i.e. HF) safely in many applications.
Overall, the research in the field of ILs is still at very early stage and many of their
properties need to be elucidated as yet. However, various permutation of cations and anions
can lead to the design of a variety of novel ILs having useful applications as well as less
toxicity besides biodegradability to microorganisms. In fact, a large number of non-toxic
and biodegradable ILs have been discovered recently [Harjani et al. (2010); Trivedi et al.
(2011)].
After discussion on ILs in organic synthesis, conservation of energy is also an important
principle of green chemistry which is discussed below:
Green Synthesis of…… General Introduction
20
1.4.5 Energy conservation:
Traditionally, in the most commonly used heating sources such as Bunsen burner, heater, oil
bath, electric plate heater or heating mantle, the transfer of heat energy into the reaction
system depends on convection currents beside thermal conductivity of various materials of
the reaction pot. Consequently, the temperature of the reaction vessel is always higher than
that of the reaction mixture which in turn can lead to the decomposition of product,
substrate or reagent due to development of temperature gradient. In the above backdrop,
MW as non conventional energy source has become very popular and useful technology in
organic chemistry [Lidstrom et al. (2001); Kappe (2004, 2008); Loupy (2006); Polshettiwar
and Varma (2008)].
1.4.5.1 Microwave:
Microwaves are basically electromagnetic radiations which fall in the frequency range from
300 Hz to 30 GHz that corresponds to the wavelengths of 1m to 1cm. To avoid the
interference with radar and telecommunications, most of the MW appliances operate at
fixed frequency of 2450 MHz. In contrast to traditional heating sources, MW irradiation
couple directly with the component of reaction mixture [Kappe (2008); Loupy (2006)].
1.4.5.1.1 Theory of Microwave:
Heat energy of MW is transferred to the reaction mixture by the following two mechanisms
[Lidstrom et al. (2001); Kappe (2008)].
(i) Dipolar polarization (ii) Ionic conduction
(i) Dipolar polarization:
In the dipolar polarization mechanism,
electric field component of MW
interacts with polar molecules of
reaction mixture. When a molecule
with dipole moment is subjected to
MW it tends to align itself with the
field by rotation (Figure 15). However,
the frequency of the rotating dipole is
not high enough to precisely follow the
alternating electric field of MW. So as the dipole re-orients to align itself with the electric
field, the field is already changing and generates a phase difference between the dipole and
the orientation of the field. As a result lot of friction leads to intense internal heating.
Figure 15. Interaction of the electric field of MW withdipole moment [Gagnon (2008)]
Electric field of MW
Dipole
Figure 15. Interaction of the electric field of MW withdipole moment [Gagnon (2008)]
Electric field of MW
Dipole
Green Synthesis of…… General Introduction
21
(ii) Ionic conduction:
In the ionic conduction mechanism, the charged particles of the sample (usually ions)
oscillate back and forth under the influence of electric component of MW irradiation. They
collide with their neighbouring molecules or atoms and these collisions cause agitation or
motion, creating heat. The ionic conduction principle is a much stronger effect than the
dipolar rotation mechanism with regard to the heat-generating capacity. That’s why the
media containing ions are heated more efficiently by MW than just polar solvents.
Since MW energy is directly imparted to the reaction medium rather than through the wall
of reaction vessel, it is an efficient source of energy than conventional steam wherein
heating the entire furnace or oil bath consumes lot of time and energy.
1.4.5.1.2 Microwave-assisted organic synthesis [MAOS]:
MAOS is considered as an important approach towards green chemistry [Basu et al. (2003);
Kappe (2008); Polshettiwar and Varma (2008)]. Since the first report on MAOS in 1986,
the technique has been accepted to reduce the reaction time besides increasing yield of
product compared to conventional synthesis. In addition, MW heating in a pressurized
system rapidly increases temperature far above the boiling point of the solvent and leads to
a uniform energy transfer to the reactants of the chemical reaction.
Some of the examples of microwave-assisted organic reactions and their comparison with
conventional methods are given in Table 2.
Table 2. Some examples of microwave-assisted reactions and their comparison with conventional conditions
Ph
COOH
Ph
Reaction Activation mode Reference
Conventional
MW
Time Yield
CHO
HO HO
6 h
5 min
Kumar etal. (2007b)
Sinha et al.(2007b)
O
Ph
Ph
O Conventional
MW
10h 0%
1 h 73%
Cleary et al.(2011)
O
O
O
O
Conventional
MW
61%
88%
16 h
10 min
Zhou et al.(2006a)
+ CH2(COOH)2
CO2Et
N
NCH2CH2CO2Et
NNH
Conventional
MW 5 minMartin-Arandaet al. (1997)
Conventional
MW
5 min 27%
75 %
12%
61%
AcO HO 20 min 87%
16 h
OCH3 OCH3
62%
+
Green Synthesis of…… General Introduction
22
Recent trends in MAOS are the use of environmentally benign ILs. Ionic liquids being salts
(feature polar and ionic character) interact efficiently with MW irradiation through both
polarization and ionic conduction energy transfer mechanisms. Thus, ILs are considered as ideal
solvents for MAOS.
1.4.6 Application of ionic liquids in microwave assisted organic synthesis:
A number of reports on the use of ILs as heating aid, solvents, co-solvents, additives and
catalysts in MAOS has been furnished in literature. Some of the selected applications of ILs
in MAOS are described below:
1.4.6.1 Ionic liquids as heating aid under microwave:
Leadbeater et al. investigated the role of ILs for the MW heating of non polar solvents such
as hexane, toluene, THF and dioxane etc [Leadbeater and Torenius (2002)] and found that
such non polar solvents can be heated far above their boiling points with the aid of small
amount of an IL. Some of the ILs used in the present study and comparison of the
temperature attained in the presence or absence of these ILs is depicted in Table 3.
N N X N N X
1. X = I,2. X = PF6 3. X = Br
THF
Solventused IL added Temperature
attained with IL(°C)
Time(S)
Temperaturewithout IL
(°C)
Boiling point(°C)
Hexane
Toluene
Dioxane
279 20217 10 46 69
228 15195 150 109 111
268 70 112 66
264 90 76 101
Table 3. The microwave heating effects of adding a small quantity of ionic liquids to hexane,toluene, THF and dioxane
234 130280 60
242 60231 60
246 90149 100
1
32
1
32
1
32
1
32
Green Synthesis of…… General Introduction
23
1.4.6.2 Ionic liquids as benign reaction media under microwave:
Some of the organic reactions wherein ILs have been used as reaction media are discussed
below:
1.4.6.2.1 For synthesis of 2,4,5-trisubstituted imidazole derivatives:
Xia et al. conducted a three component synthesis of 2,4,5-trisubstituted imidazole
derivatives in a neutral ionic liquid, 1-methyl-3-heptylimidazolium tetrafluoroborate
([hemim]BF4) under MW (Scheme 7) [Xia and Lu (2007)]. The reaction got completed
within 2-6 min of MW irradiation whereas conventional heating (oil bath) took 2 h for its
completion besides providing the product in comparatively low yield.
O
O
CHO
NH
N+
MW (135 W)
NH4OAc[hemim]BF4
R
R
R =H, F, Cl, Br, CF3, CH3, OH etc.
(2-6 min)
Scheme 7
1.4.6.2.2 For Heck reaction:
Heck reaction is a transition metal catalyzed cross coupling arylation of olefins with organic
halides and is an important method for the construction of C-C bond. Heck reaction has
been studied by various groups under ILs [Bellina and Chiappe (2010)] or IL-MW
conditions. For instance, Vallin et al. studied the Heck coupling of aryl halides with n-butyl
acrylate using PdCl2 along with ligand P(o-tol)3 and base Et3N in [bmim]PF6 under MW
condition (Scheme 8) [Vallin et al. (2002)]. Similarly, Heck coupling of aryl halides with
styrenes or n-butyl acrylate using Pd/C and (n-Bu)3N without any ligand has been carried
out in [omim]BF4 under MW irradiation [Xie et al. (2004)]. The reaction time was reduced
significantly using IL-MW combination
X R'
+R
PdCl2, [bmim]PF6, Et3NP(o-tol)3 (5-20 min)
MicrowaveR
Pd/C, [omim]BF4, (n-Bu)3N (1.5-2.0 min)
R'
R = H, OCH3 etc.X = Br, I
R' = COOBu
R' = COOBu or Ph
Scheme 8
Green Synthesis of…… General Introduction
24
1.4.6.2.3 For oxidation of alcohols:
Development of cost-effective and environmentally benign oxidation process, particularly
using H2O2 holds a prominent place among other oxidants [Wahlen et al. (2003); Kumar et
al. (2007a)]. However, oxidation with H2O2 requires prior activation by transition metal
catalysts due to its low activation energy. Consequently, various transition metal complexes
for H2O2 assisted oxidation of alcohols in ILs (such as [bmim]Br, [bmim]BF4) under
conventional heating have been studied by a number of groups [Muzart (2006)]. Also,
combination of IL and MW has been employed for the oxidation of various alcohols using KIO4
as an oxidant (Scheme 9) [Hajipour et al. (2006)].
R1 R2
OH
R1 R2
O
MW
R1 = R2 = H, Ph etc.
KIO4, Et4NBr
Scheme 9
To the best of our knowledge, oxidation of alcohols with green oxidant H2O2 without any
transition metal catalyst using IL-MW combination has not yet been explored.
1.4.6.2.4 For Stetter reaction:
Zhou et al. studied the combination of [bmim]BF4 and MW irradiation for the
intramolecular Stetter reaction for the synthesis of chromanone derivatives [Zhou et al.
(2006b)]. The reactions were also performed under conventional heating using IL as a
solvent (Table 4). The results show that IL-MW condition significantly reduced the reaction
time from 8 h to 20 min besides improvement in the yield of the products.
OO
O
O
O
OO
O
Et3N, [bm im]BF4
N
S
CH3H3C
BrHO
IL-conventional IL-MW
Table 4. Microwave-assisted intramolecular Stetter reaction using ionic liquid as a solvent
Entry
12
Time Yield Time Yield
5 h2.5 h8 h
808874
20 min5 min20 min
919484
RR
R
HClOCH33
Green Synthesis of…… General Introduction
25
The use of ILs under MW irradiation for various organic reactions is summarized at the end
i.e. in section 1.4.6.7.
There has been much recent interest in developing ‘catalyst free’ organic transformations in
neat ILs, wherein, the classical necessity of an additional metal or acid/base catalyst stands
removed [Meciarova et al. (2007); Parvulescu and Hardacre (2007)]. Consequently, a
number of reports on the applications of such TSILs, whereby the role of the IL goes
beyond that of a solvent have been furnished in literature.
1.4.6.3 Ionic liquids as solvent cum catalysts under microwave:
1.4.6.3.1 For decarboxylation of aromatic acids:
Sharma et al. have shown that ILs can be used as efficient catalysts cum solvents for
decarboxylation of diverse N-heteroaryl and aryl carboxylic acids under MW irradiation
without using any metal or base quinoline (Scheme 10) [Sharma et al. (2008)]. The
developed methodology offers several inherent advantages like reduction in waste,
recyclability of reagent system, short reaction times besides ease of product recovery.
HOOC
N
R
R=R'= H, CH3, X = H, Cl
R'OR3
R1R2
R4
R1-R4 = OMe, OH etc.R' = H, CH3 etc.
HOR3
R1
R2
R4
R5 R7
R8
R9
R6
R1-R9 = H, OMe, OH etc.
x
Ionic Liquid-WaterMW
R = Indoles, Aryl ethenes andArene derivatives
R
X= OH, NO2, ClR = H, CH3, CH2-C6H5 etc.
R
X
CO2
R'
Scheme 10
1.4.6.3.2 For Michael addition and alkylation of active methylene compounds:
Ranu et al. studied the use of basic ionic liquid [bmim]OH for the Michael addition of
active methylene compounds to conjugated -unsaturated ketones, carboxylic acids,
esters and nitriles besides alkylation of active methylene compounds (Scheme 11) [Ranu et
al. (2007a)]. Thus, it is observed that alkylation reaction of active methylene compounds
with alkyl halides proceed efficiently under MW irradiation within 5 min. The open chain
Green Synthesis of…… General Introduction
26
active methylene compounds (such as 1,3-diketones or 1,3-keto carboxylic esters) produced
the monoalkylated products, whereas the cyclic diketones provided the dialkylated products.
On the other side, conventional heating (80-100°C) for more than 12 h provided the
products in inferior yields.
R1
R2W
R1
R2
R1
R2
W
W
R1
R2
R3XR1
R2
R3R1
R2
COOMe
R1 = R2 = COOMe, COOEt, CN, NO2R3 = alkyl, benzyl, allyl, propargylX = Br, I
R3
R3
+ or[bmim]OH
+
W = COR
W = CN
[bmim]OH
MW (100°C)
W
Scheme 11
The same group has also investigated the stereoselective debromination of vicinal
dibromides [Ranu and Jana (2005)] and -bromoketones [Ranu et al. (2007b)] using ionic
liquid [pmim]BF4 under MW irradiation. In both cases [pmim]BF4 was found to be an
efficient catalyst cum solvent.
1.4.6.3.3 For Pechmann reaction:
The Pechmann reaction (condensation of phenols with β-ketoesters) for the synthesis of
coumarins generally involves acid catalysts such as H2SO4, P2O5, AlCl3 etc. In this context,
ionic liquid [bmim]HSO4 has been effectively utilized for the synthesis of coumarins under
MW (Scheme 12) [Singh et al. (2005)]. A comparison of MW and conventional heating was
also carried out, wherein, MW proved better in terms of yields and reaction times.
O OOH
O
OR'
OR R+
M.W (140 W)(2-10 min)
[bmim]HSO4
R = OH, CH3 etc.R' = CH3, C2H5
Scheme 12
Green Synthesis of…… General Introduction
27
1.4.6.4 Ionic liquids and microwave in biocatalysis:
ILs are promising as media for enzymatic transformations, allowing high levels of
efficiency and good solubility [Sheldon et al. (2002); Jain et al. (2005); Sunitha et al.
(2007)]. Major et al. investigated the enzymatic esterification of lactic acid with ethanol in
IL medium under MW conditions (Scheme 13) [Major et al. (2009)]. MW irradiations were
found to cause hydrolysis of lactoyllactic acid (the linear dimer of lactic acid) and as a
result, more lactic acid was found available as substrate for enzyme (CALB) enabling better
yield of product.
H3C
OH
OH
O
H3C OH H3CCH
OH
O
O
CH3
MW (10W, 40°C)
Scheme 13
Lipase, ionic liquid+
Recently, IL-MW combination has been successfully explored for the pretreatment of
cellulosic biomass to make it more accessible to enzymatic hydrolysis [Ha et al. (2011)].
1.4.6.5 Deuterolabeling of polyphenols using ionic liquid and microwave:
Deuterolabeling of biologically active compounds is highly desired because such labeled
analogues are used as internal standards for quantitation of biological samples, screening of
biological activities besides determining the biosynthetic and metabolic pathways. In this
context, IL-MW combination has been effectively employed for postsynthetic regioselective
aromatic ring H/D exchanges (using DCl/D2O) in bioactive polyphenolic compounds
(Scheme 14) [Hakala and Wahala (2007)].
Unlabled compound[bmim]Cl + DCl/D2O(MW, 15-40 min)
e.g.O
O
D
D
HOD
D
DD OH
Scheme 14
Green Synthesis of…… General Introduction
28
1.4.6.6 Ionic liquid and microwave for nanoparticles synthesis:
The low surface tension of many ILs leads to high nucleation rates and thus offers
outstanding properties as media for the synthesis of nanoparticles. In this context an
efficient microwave-assisted preparation of magnetite nanoparticles is described in ionic
liquid [bmim]BF4 (Scheme 15) [Hu et al. (2010)]. The use of IL was found to be an
efficient aid for MW heating of non-polar dibenzyl ether in high temperature solution-phase
synthesis of monodisperse magnetite nanoparticles. The developed IL-MW technology
provided lot of advantages over conventional protocols which usually proceed via co-
precipitation of Fe2+ and Fe3+ ions by a base (e.g. NaOH, NH3.H2O etc) for which careful
adjustment of pH is required.
O
O
N NBF4
Fe3O4
Fe(acac)3
RCOOH
ROH
Microwave
Good microwave absorption(Efficient synthesis of nanoparticles)
Poor microwave absorption(inefficient synthesis of nanoparticles)
Scheme 15
1.4.6.7 Applications of ionic liquids and microwave combination for some of the organic
name reactions:
The application of IL-MW technology for various name reactions is summarised in Table 5
[Palou (2010)].
Green Synthesis of…… General Introduction
29
Name of the reaction Catalyst/IL-MW conditions Reference
Diels-Alder cycloaddition
S.No.
1
Mannich condensation
Knoevenagel condensation
Biginelli
Pictet–Spengler
Friedel–Craft (acylation)
Beckmann Rearrangement
Heck coupling
Morita–Baylis–Hillman
Pechmann
[bmim]Cl–AlCl3
[bmim]HSO4
Bis{(trifluoromethyl)sulfonyl}amine(HNTf2) or BF3-Et2O/[bmim]BF4
[bmim]HSO4
Tsuji–Trost Pd(OAc)2/[emim]BF4/H2O
[bmim]BF4
CuCl/[i-ProMIM]PF6
Organotungsten catalyst/[bmim]PF6Mineral supports/[hmim]BF4
In(OTf)/[bdmim]PF6
H2O/DABCO/[bmim]PF6
Pd/C/[omim]BF4
2
3
4
5
6
7
8
9
10
11
12
Leadbeater et al. (2003)
Arfan et al. (2007)
Liao et al. (2005)
Srinivasan and Ganesan(2003)
Singh et al. (2005)
Hakala and Wahala(2006)
de Souza et al. (2008)
Xie et al. (2004)
Sugamoto et al. (2011)
Ma et al. (2006)
Chen et al. (2004)López et al. (2007)
Fisher esterification [bmim]HSO4Arfan and Bazureau(2005)
Table 5. Applications of ionic liquids and microwave for some selected organic reactions
1.5 Objectives of present studies:
In view of the above discussion, it is evident that synergistic combination of ILs with MW
is a useful concept for the designing of environmentally benign chemical processes. The
thesis entitled “Green Synthesis of Bioactive Phenolics Employing Ionic Liquids and
Microwave Assisted Approaches” is mainly concerned with the development of alternative
environmental benign strategies for the synthesis of phenolic compounds or their chemical
modification into other value added products either using ILs or MW irradiation. ILs have
been used as solvent, co-solvent besides exploring their dual role as solvent-cum catalyst.
Moreover, use of ILs in conjunction with MAOS has led to the expeditious synthesis of
biologically important phenolics such as arylalkenes, arylalkanones, stilbenoids and
Green Synthesis of…… General Introduction
30
chalcones etc. Also, various synthesized chalcone derivatives have been evaluated for their
antimalarial and pesticidal potential.
For the sake of convenience, the studies undertaken in this thesis have been divided into
four chapters as mentioned below:
Chapter 1 Ionic liquid and microwave assisted dehydration of arylalkanols into (E)-
arylalkenes under neutral condition
Chapter 2 Synergism of ionic liquid and microwave towards metal-free activation of
H2O2 for oxidation of benzyl alcohols
Chapter 3 Structure-activity relationship of chalcones and their derivatives for
antimalarial and pesticidal activity
Chapter 4 Microwave promoted tandem C-C bond formation strategy in ionic liquid for
synthesis of stilbenoids
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