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Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2

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Page 1: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2
Page 2: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2
Page 3: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2

Glucosyltransferases

O

O

O

O

OHO

O

Polymer synthesis

OHO

HOHO

HO

HO

HOOH

OHOO

+

OHO

OHO

Hydrolysis

Acceptor reactions

HOHO

HOHO

HOHO

HO

HOHO

HO

HO

HO

HO

HO

HO

HOHO

HOHO

HOHO

HO

HO

HOHO

HO

HO

HO

HO

HO

HO

HOHO

HOHO

HOHO

HOHO

HO

HO

HO

O

O

O

O

OH

OH

OH

OHO

OO

O

O

HOMaltose

Sucrose

Dextran

Glucose

+

+

Panose

Fructose

Fructose

Fructose

n

Page 4: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2

Alternan

Mutan

Dextran

Glucosyltransferases

Leuconostoc mesenteroides

Streptococcus

linkages

1-6B-512F

B-1299

B-1355

1-3

1-2

mutans GS5

downei Mfe28

Microorganism enzyme

Dextransucrase

Alternansucrase

Mutansucrase

Glucansucrase

1-6

1-6 1-3

1-6 1-3

1-6 1-3

strain polymer

Glucan

Page 5: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2
Page 6: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2

O

O

OO

O

O

O

CH2OH

CH2OHCH2

OHOH

OH

OH

OH

OH

OH

OHOH

HOCH2

Page 7: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2

Oligosacaridos

-Fructo-oligosacáridos

-Fructo-oligosacáridos

-Galacto-oligosacáridos

-Galacto-oligosacáridos

Lactulosa

-Gluco-oligosacáridos

-Gluco-oligosacáridos

-Gluco-oligosacáridos

Xylo-oligosacáridos

Proceso

Síntesis Enzimática

Hidrólisis Enzimática

(inulina)

Extracción de Soya

Síntesis Enzim{atica

Isomerización de lactosa

Síntesis Enzimática

Hidrólisis de Almidón

Síntesis Enzimática

Hidrólisis Enzimática

(Hemicelulosa)

Empresa

Meiji Seika

Refinería

Tirlemontoise

Calpis

Yakult

Nikken Kapau

Meiji Seika

Showa Sangyo

BioEurope

fuente: Monsan P. Biofutur, (6,7), 1993

Page 8: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2

Bioprospection

Agave PozolPulque

Page 9: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2

Pozol

14 strains of Leuconostoc mesenteroides

Cell-associated activity

Identify as Leuconostoc citreum by analysis of 16S

rRNA

screening of glucosyltransferases activities

FRUCTOSYLTRANSFERASE

Page 10: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2

o

Fructose

ooHO

HO

OHHO

HO

OHHO

OH

o

HO

HOHO

HO o

Sucrose

Polymer synthesis

(n)

O O OO

o

HO

HO

HOCH2

O O

HO

HO CH2OHCH2OH

CH2 CH2

CH2OHHO

HO

O CH2

CH2OHHO

HO

O

CH2

HO

HO

HOHO

HO

HOHO

HO o

OH

+

Levan

Glucose

Acceptor reactions

Fructosyltransferases

HO

HOHO

HO o

OH

Glucose

+

Hydrolisis

+HO

HOHO

HO o

OH

Glucose

HOHO

HOHO

HO

HO

HO

O

OO

HO

Maltose

HOHO

HOHO

HO

HO

HO

O

OO

OO

HO

HO

OHHO

Fructoside

Page 11: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2

ß-2,1 linked fructosyl moieties with branching through ß-2,6 linkages

(n)

O

o

HO

HO

O

CH2

HO

HO

HOHO

CH2

CH2OH

O

HO

HO

O

CH2

CH2OH

O

HO

HO

OCH2OH

CH2OH

Inulin

Levan

ß-2,6 linked fructosyl moieties with branching through ß-2,1 linkages

O O OO

o

HO

HO

HOCH2

O O

HO

HOCH2OHCH

2OH

CH2

CH2

CH2OHHO

HO

O CH2

CH2OHHO

HO

O

CH2

HO

HO

HOHO

(n)

Page 12: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2

Applications of fructans in food

• Technological properties

– Taste improvement

– Texturizing agent

(mouthfeel)

– Sugar and fat

replacement

– Filler/binder in tablets

• Nutritional properties

– Soluble dietary fiber

– Prebiotic and related

health effects

– Sugar and fat replacement

– Low caloric value

– Suitable for diabetics

Page 13: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2

Fructosiltransferasas

• Plantas

Neoseries de levana

LEVANALEVANA

levana

Neoseries de inulina

INULINAINULINA

levana

NEOKESTOSA

1- KESTOSA

BIFURCOSA

SACAROSA6-KESTOSA

6-SFT

6-SFT

6-SFT 6-SFT

1-FFT

1-FFT

1-FFT

6-SFT

6G-FFT

1-SST

+ Sac

+ Sac

Modelo de la biosíntesis de fructanas en plantas (Vijn y Smeekens, 1999).

©SCIMAT 2003

• Bacterias

SACAROSA

INULINAINULINA

LEVANALEVANA

IS

LS

http://www.promza.org.ar/export/ingles/m22a.htm
http://images.google.com.mx/imgres?imgurl=http://www12.ocn.ne.jp/~pageon/MyPage/img/Tulipa_gesneriana.jpg&imgrefurl=http://jolly-ocn.blog.ocn.ne.jp/y/cat61078/&h=240&w=320&sz=16&tbnid=uUS37MGb9kUJ:&tbnh=84&tbnw=113&hl=es&start=4&prev=/images%3Fq%3Dtulipa%2Bgesneriana%26svnum%3D10%26hl%3Des%26lr%3D%26sa%3DN
Page 14: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2

Azucar Caña de azúcar

Enzima

Fructosiltransferasa

Glucosa

Fructosa

Fructosa

Fructosa

Fructosa

FOS

Glucosa

+

Fructosa

Sacarosa =

Page 15: Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2

1 tea spoon of NutraFlora

22 bananas15 onions383 cloves of garlic


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