Figure S1. Analysis by EMSA of competition by excess unlabelled probe of binding of PhoP to the glnA, glnII and amtB promoters. P: probe without protein; PhoP: probe with GST-PhoPDBD protein (0.5 µM ); C: Control with GST-PhoPDBD protein (0.5 µM) and an excess of unlabeled probe. Note the competition with the labeled probe in the formation of the shifted band.

glnII

P PhoP

C

glnA

P PhoP

C

amtB

P PhoP

C