GILSON 203B204

Clarity Control Module ENG

CodeRev M16280ADate 392020

Phone +420251013400 DataApex Ltd

Fax +420251013401 Petrzilkova 258313

claritydataapexcom 15800Prague 5

wwwdataapexcom TheCzech Republic

Clarityreg DataApexreg and reg are trademarks of DataApex Ltd Microsoft reg and WindowsTM aretrademarks of Microsoft CorporationDataApex reserves the right to make changes to manuals without prior notice Updated manuals can bedownloaded fromwwwdataapexcom

Author DM

Gilson 203B204 Table of Contents

Contents1 Gilson 203B204 Control Module 12 Requirements 23 Installation Procedure 331 Gilson 203B204 fraction collector communication 332 Clarity Configuration 4

4 Using the control module 641 Method Setup - FC - Fraction Table 742 Method Setup - FC - Vial Numbers 1243 Hardware Configuration 1444 Device Monitor 1545 Data Acquisition window 1746 DataApex UNI Setup 18

5 Report Setup 206 Troubleshooting 21

- i -

Gilson 203B204 Table of Contents

To facilitate the orientation in the Gilson 203B204 manual and Clarity chromatography stationdifferent fonts are used throughout themanual Meanings of these fonts are

Instrument (blue text) marks the nameof thewindow to which the text refersOpen File (italics) describes the commands and names of fields in Clarity parameters that canbe entered into themor a window or dialog name (when you already are in the topic describingthewindow)WORK1 (capitals) indicates the nameof the file andor directoryACTIVE (capital italics) marks the state of the station or its part

The bold text is sometimes also used for important parts of the text and the name of the Claritystation Moreover some sections are written in format other than normal text These sections areformatted as follows

Note Notifies the reader of relevant information

Caution Warns the user of possibly dangerousor very important information

Marks the problem statement or trouble questionDescription Presents more detailed information on the problem describes its causes

etcSolution Marks the response to the question presents a procedure how to remove it

- ii -

Clarityreg DataApexreg and reg are trademarks of DataApex Ltd Microsoft reg and WindowsTM aretrademarks of Microsoft CorporationDataApex reserves the right to make changes to manuals without prior notice Updated manuals can bedownloaded fromwwwdataapexcom

Author DM

Gilson 203B204 Table of Contents

Contents1 Gilson 203B204 Control Module 12 Requirements 23 Installation Procedure 331 Gilson 203B204 fraction collector communication 332 Clarity Configuration 4

4 Using the control module 641 Method Setup - FC - Fraction Table 742 Method Setup - FC - Vial Numbers 1243 Hardware Configuration 1444 Device Monitor 1545 Data Acquisition window 1746 DataApex UNI Setup 18

5 Report Setup 206 Troubleshooting 21

- i -

Gilson 203B204 Table of Contents

To facilitate the orientation in the Gilson 203B204 manual and Clarity chromatography stationdifferent fonts are used throughout themanual Meanings of these fonts are

Instrument (blue text) marks the nameof thewindow to which the text refersOpen File (italics) describes the commands and names of fields in Clarity parameters that canbe entered into themor a window or dialog name (when you already are in the topic describingthewindow)WORK1 (capitals) indicates the nameof the file andor directoryACTIVE (capital italics) marks the state of the station or its part

The bold text is sometimes also used for important parts of the text and the name of the Claritystation Moreover some sections are written in format other than normal text These sections areformatted as follows

Note Notifies the reader of relevant information

Caution Warns the user of possibly dangerousor very important information

Marks the problem statement or trouble questionDescription Presents more detailed information on the problem describes its causes

etcSolution Marks the response to the question presents a procedure how to remove it

- ii -

Gilson 203B204 Table of Contents

Contents1 Gilson 203B204 Control Module 12 Requirements 23 Installation Procedure 331 Gilson 203B204 fraction collector communication 332 Clarity Configuration 4

4 Using the control module 641 Method Setup - FC - Fraction Table 742 Method Setup - FC - Vial Numbers 1243 Hardware Configuration 1444 Device Monitor 1545 Data Acquisition window 1746 DataApex UNI Setup 18

5 Report Setup 206 Troubleshooting 21

- i -

Gilson 203B204 Table of Contents

To facilitate the orientation in the Gilson 203B204 manual and Clarity chromatography stationdifferent fonts are used throughout themanual Meanings of these fonts are

Instrument (blue text) marks the nameof thewindow to which the text refersOpen File (italics) describes the commands and names of fields in Clarity parameters that canbe entered into themor a window or dialog name (when you already are in the topic describingthewindow)WORK1 (capitals) indicates the nameof the file andor directoryACTIVE (capital italics) marks the state of the station or its part

The bold text is sometimes also used for important parts of the text and the name of the Claritystation Moreover some sections are written in format other than normal text These sections areformatted as follows

Note Notifies the reader of relevant information

Caution Warns the user of possibly dangerousor very important information

Marks the problem statement or trouble questionDescription Presents more detailed information on the problem describes its causes

etcSolution Marks the response to the question presents a procedure how to remove it

- ii -

Gilson 203B204 Table of Contents

To facilitate the orientation in the Gilson 203B204 manual and Clarity chromatography stationdifferent fonts are used throughout themanual Meanings of these fonts are

Instrument (blue text) marks the nameof thewindow to which the text refersOpen File (italics) describes the commands and names of fields in Clarity parameters that canbe entered into themor a window or dialog name (when you already are in the topic describingthewindow)WORK1 (capitals) indicates the nameof the file andor directoryACTIVE (capital italics) marks the state of the station or its part

The bold text is sometimes also used for important parts of the text and the name of the Claritystation Moreover some sections are written in format other than normal text These sections areformatted as follows

Note Notifies the reader of relevant information

Caution Warns the user of possibly dangerousor very important information

Marks the problem statement or trouble questionDescription Presents more detailed information on the problem describes its causes

etcSolution Marks the response to the question presents a procedure how to remove it

- ii -

Gilson 203B204 1Gilson 203B204 ControlModule

1 Gilson 203B204 Control ModuleThis manual describes the setting of the Gilson 203B204 fractioncollector The control module enables direct control of the instrument overserial line

Fig 1Gilson 203B204 fraction collector

Direct control means that the fraction collector can be completelycontrolled from the Clarity environment The Instrument methodcontrolling the analysis conditions will be saved in the measuredchromatogramsThe control is performed via the UNI Ruby control module and the Gilson203B204 script

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Gilson 203B204 2 Requirements

2 RequirementsClarity Installation USB with appropriate control license allowed - LCControl (pn A24) or GC Control module (pn A23) for thermostats valvesand detectors LC Control (pn A24) for pumps and fraction collectors GCControl module (pn A23) for gas chromatographs or AS Control module(pn A26) for autosamplersFree serial COM port in the PC

Note Modern computers usually have only one (if any) serial (COM) portinstalled To use more devices requiring the RS232 port the MultiCOMadapter (pnMC01) is available

RS232 to GSIOC cable converter (pn IGLN1) or Gilson equivalent

Note Cables are not part of Clarity Control Module It is stronglyrecommended that you order the required cables together with theControlModule

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Gilson 203B204 3 Installation Procedure

3 Installation Procedure31 Gilson 203B204 fraction collector communication

The Gilson 203B204 is controlled by GSIOC communication Clarityuses GSIOC to RS232 converter to be able to use the standard COMports

Note Even though the IGLN1 kit has possible connections to two Gilsoninstruments when using the Gilson 203B204 fraction collector thesecond socket must be left empty

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Gilson 203B204 3 Installation Procedure

32 Clarity Configuration

Fig 2 How to Add UNI Rubymodule

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Gilson 203B204 3 Installation Procedure

Start the Clarity station by clicking on the icon on the desktopInvoke the System Configuration dialog accessible from the Claritywindow using the System - Configuration commandPress the Add button (① on Fig 2 on pg 4) to invoke the Available ControlModules dialogYou can specify the search filter② to simplify the finding of the driverSelect the correct item and press the Add (③ on Fig 2 on pg 4) buttonEach device with already created UNI profile should have its own itemnamed accordingly in the Available Control Modules dialogThe DataApex UNI Setup dialog will appear

Fig 3 Gislon 203B setup

Set the correct communication Port and click on the AutoDetect button toestablish communication with the deviceYou may fill in the custom Device Name

Note The DataApex UNI Setup dialog is described in detail in the chapterDataApex UNI Setup on pg 18

The Gilson 203B204 item④ will appear in the Setup Control Modules listof the System Configuration dialogDrag the appropriate item from the Setup Control Modules list on the leftside to the desired Instrument tab ⑤ on the right side ⑥ or click on the

button ⑦ Set the Ext Start Dig Input and Ready Dig Output numbers ⑧ for youracquisition card according to the wires used for synchronization

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Gilson 203B204 2 Requirements

2 RequirementsClarity Installation USB with appropriate control license allowed - LCControl (pn A24) or GC Control module (pn A23) for thermostats valvesand detectors LC Control (pn A24) for pumps and fraction collectors GCControl module (pn A23) for gas chromatographs or AS Control module(pn A26) for autosamplersFree serial COM port in the PC

Note Modern computers usually have only one (if any) serial (COM) portinstalled To use more devices requiring the RS232 port the MultiCOMadapter (pnMC01) is available

RS232 to GSIOC cable converter (pn IGLN1) or Gilson equivalent

Note Cables are not part of Clarity Control Module It is stronglyrecommended that you order the required cables together with theControlModule

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Gilson 203B204 3 Installation Procedure

3 Installation Procedure31 Gilson 203B204 fraction collector communication

The Gilson 203B204 is controlled by GSIOC communication Clarityuses GSIOC to RS232 converter to be able to use the standard COMports

Note Even though the IGLN1 kit has possible connections to two Gilsoninstruments when using the Gilson 203B204 fraction collector thesecond socket must be left empty

- 3 -

Gilson 203B204 3 Installation Procedure

32 Clarity Configuration

Fig 2 How to Add UNI Rubymodule

- 4 -

Gilson 203B204 3 Installation Procedure

Start the Clarity station by clicking on the icon on the desktopInvoke the System Configuration dialog accessible from the Claritywindow using the System - Configuration commandPress the Add button (① on Fig 2 on pg 4) to invoke the Available ControlModules dialogYou can specify the search filter② to simplify the finding of the driverSelect the correct item and press the Add (③ on Fig 2 on pg 4) buttonEach device with already created UNI profile should have its own itemnamed accordingly in the Available Control Modules dialogThe DataApex UNI Setup dialog will appear

Fig 3 Gislon 203B setup

Set the correct communication Port and click on the AutoDetect button toestablish communication with the deviceYou may fill in the custom Device Name

Note The DataApex UNI Setup dialog is described in detail in the chapterDataApex UNI Setup on pg 18

The Gilson 203B204 item④ will appear in the Setup Control Modules listof the System Configuration dialogDrag the appropriate item from the Setup Control Modules list on the leftside to the desired Instrument tab ⑤ on the right side ⑥ or click on the

button ⑦ Set the Ext Start Dig Input and Ready Dig Output numbers ⑧ for youracquisition card according to the wires used for synchronization

- 5 -

Gilson 203B204 3 Installation Procedure

3 Installation Procedure31 Gilson 203B204 fraction collector communication

The Gilson 203B204 is controlled by GSIOC communication Clarityuses GSIOC to RS232 converter to be able to use the standard COMports

Note Even though the IGLN1 kit has possible connections to two Gilsoninstruments when using the Gilson 203B204 fraction collector thesecond socket must be left empty

- 3 -

Gilson 203B204 3 Installation Procedure

32 Clarity Configuration

Fig 2 How to Add UNI Rubymodule

- 4 -

Gilson 203B204 3 Installation Procedure

Start the Clarity station by clicking on the icon on the desktopInvoke the System Configuration dialog accessible from the Claritywindow using the System - Configuration commandPress the Add button (① on Fig 2 on pg 4) to invoke the Available ControlModules dialogYou can specify the search filter② to simplify the finding of the driverSelect the correct item and press the Add (③ on Fig 2 on pg 4) buttonEach device with already created UNI profile should have its own itemnamed accordingly in the Available Control Modules dialogThe DataApex UNI Setup dialog will appear

Fig 3 Gislon 203B setup

Set the correct communication Port and click on the AutoDetect button toestablish communication with the deviceYou may fill in the custom Device Name

Note The DataApex UNI Setup dialog is described in detail in the chapterDataApex UNI Setup on pg 18

The Gilson 203B204 item④ will appear in the Setup Control Modules listof the System Configuration dialogDrag the appropriate item from the Setup Control Modules list on the leftside to the desired Instrument tab ⑤ on the right side ⑥ or click on the

button ⑦ Set the Ext Start Dig Input and Ready Dig Output numbers ⑧ for youracquisition card according to the wires used for synchronization

- 5 -

Gilson 203B204 3 Installation Procedure

32 Clarity Configuration

Fig 2 How to Add UNI Rubymodule

- 4 -

Gilson 203B204 3 Installation Procedure

Start the Clarity station by clicking on the icon on the desktopInvoke the System Configuration dialog accessible from the Claritywindow using the System - Configuration commandPress the Add button (① on Fig 2 on pg 4) to invoke the Available ControlModules dialogYou can specify the search filter② to simplify the finding of the driverSelect the correct item and press the Add (③ on Fig 2 on pg 4) buttonEach device with already created UNI profile should have its own itemnamed accordingly in the Available Control Modules dialogThe DataApex UNI Setup dialog will appear

Fig 3 Gislon 203B setup

Set the correct communication Port and click on the AutoDetect button toestablish communication with the deviceYou may fill in the custom Device Name

Note The DataApex UNI Setup dialog is described in detail in the chapterDataApex UNI Setup on pg 18

The Gilson 203B204 item④ will appear in the Setup Control Modules listof the System Configuration dialogDrag the appropriate item from the Setup Control Modules list on the leftside to the desired Instrument tab ⑤ on the right side ⑥ or click on the

button ⑦ Set the Ext Start Dig Input and Ready Dig Output numbers ⑧ for youracquisition card according to the wires used for synchronization

- 5 -

Gilson 203B204 3 Installation Procedure

Start the Clarity station by clicking on the icon on the desktopInvoke the System Configuration dialog accessible from the Claritywindow using the System - Configuration commandPress the Add button (① on Fig 2 on pg 4) to invoke the Available ControlModules dialogYou can specify the search filter② to simplify the finding of the driverSelect the correct item and press the Add (③ on Fig 2 on pg 4) buttonEach device with already created UNI profile should have its own itemnamed accordingly in the Available Control Modules dialogThe DataApex UNI Setup dialog will appear

Fig 3 Gislon 203B setup

Set the correct communication Port and click on the AutoDetect button toestablish communication with the deviceYou may fill in the custom Device Name

Note The DataApex UNI Setup dialog is described in detail in the chapterDataApex UNI Setup on pg 18

The Gilson 203B204 item④ will appear in the Setup Control Modules listof the System Configuration dialogDrag the appropriate item from the Setup Control Modules list on the leftside to the desired Instrument tab ⑤ on the right side ⑥ or click on the

button ⑦ Set the Ext Start Dig Input and Ready Dig Output numbers ⑧ for youracquisition card according to the wires used for synchronization

- 5 -

Gilson 203B204 4 Using the controlmodule

4 Using the control moduleAfter adding and setting up the new device one or more new tabs willappear in the Method Setup dialog depending on the type of theinstrument A new Gilson 203B204 fraction collector section enabling themonitoring of the current fraction collector state will be also created in theDevice Monitor window

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Gilson 203B204 4 Using the controlmodule

41 Method Setup - FC - Fraction TableThe Method Setup - FC - Fraction Table tab is used for setting theautomatic fraction collection program of the Gilson 203B204 fractioncollector The actions of the fraction collector can be displayed in DataAcquisition window or Chromatogram window click the right mousebutton on the graph choose Propertieshellip to open the Graph Propertiesdialog and check the Show Events option

Fig 4 Method Setup - FC - Fraction Table

Flow RateSets the flow rate of the liquid phase going to the fraction collector Thisparameter together with the Fraction Volume parameter from the FractionTable helps to prevent collection vial overflowing Flow rate units aretaken from the Instrument Method Sending dialogDelay Volume Detector - ValveSets the volume of the tubing between the detector and wastecollectvalve of the fraction collector Volume units are devised from the FlowRate Units set in the Instrument Method Sending dialogThe delayed volume is displayed in the Data Acquisition window as avertical line trailing the signal frontDelay Volume Valve - VialSets the volume of the tubing between the wastecollect valve and the vialin the fraction collector tray Volume units are devised from the Flow RateUnits set in the Instrument Method Sending dialog If the fraction collector

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Gilson 203B204 4 Using the controlmodule

has no wastecollect valve this field should be left empty and he totaltubing volume between the detector and fraction collector tray should betyped into the Delay Volume Detector - Valve fieldFraction TableAllows to set the fraction collector behavior The table rows are run in timeorder the time intervals should not overlap If time interval on any rowends while the fraction collector is collecting the collection will beterminated Fraction collection based on negative peaks is not supported -in case you would like to perform such fraction collection it is necessary toinvert the detector signal prior to setting up the methodNameThis name will be displayed in the Special Results table for allfractions collected in the given intervalStart Time [min]Beginning of the time interval where the Signal Condition will beevaluated See the description of Signal Condition belowStop Time [min]End of the time interval where the Signal Condition will be evaluatedWhen the Stop Time is reached the fraction collection will beterminated regardless on the current Signal Condition state See thedescription of Signal Condition belowFraction VolumeCollected amount can be collected to one or more vialsfractions TheFraction Volume value should be lower or equal to the vial volume toprevent vial overflow If the collected volume exceeds the set valuethe vial will be automatically changed Fraction Volume units aredevised from the Flow Rate Units set in the Instrument MethodSending dialogDetector SignalSelects the detector used for triggering the collection of fractionsaccording to the Signal Condition in the fraction table Each row of thetable can be related to a different detector signal Only the signals thatare enabled using the Enabled checkbox on the Method Setup -Acquisition tab of the particular detector will work in the Fraction tableSignal ConditionSignal Condition is evaluated within time interval set for each row inthe Fraction Table If these conditions are met more than once in agiven time interval the fraction collection will be performed more thanonce swapping the vial after each collection stop If the start conditionsare not met in the interval defined on fraction table row the fractioncollection will not occur in given time interval

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Gilson 203B204 4 Using the controlmodule

None - The fractions are collected in the defined time interval ruled byStart Time and Stop Time regardless of signal courseLevel - The fraction collection

starts when the detector signal increases above the Level Startstops when the detector signal decreases below the Level Stop

Slope - The fraction collection

starts when the first derivative of the detector signal increases abovethe Slope Startstops when the first derivative of the detector signal first decreasesand then increases above the negative of the Slope Stop Followingimage displays the course of the first derivative of the signal anddemonstrates Slope Stop activation Read carefully the values of theslope in all End Fraction points and the course of the first derivativebefore it reaches these End Fraction pointsit is necessary to know that for fraction collection stop that shouldcaused by Slope Stop value is applied a multiplying coefficient 105 toentered slope value (entered value 0100 recalculated to 0105)this multiplication of Slope Stop value (ie inflection point on the fallingedge of the peak) is applied in order to have correct end of the fractionwhere the Slope Stop condition is fulfilledfor easier understanding how Slope Stop value is applied inchromatogram refer to next image

Fig 5 Fist derivate course

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Gilson 203B204 4 Using the controlmodule

Note It is strongly recommended not to use Slope Signal Condition for fractioncollection based on noisy detector signal

Start L+S End L+S - The fraction collection

starts when both start signal conditions are fulfilled simultaneouslydetector signal increases above Level Start value and also the firstderivative of the detector signal increases above Slope Start value Ifonly one of the start signal conditions is fulfilled the fraction collectionwill not startstops when both stop signal conditions are fulfilled simultaneouslydetector signal decreases below Level Stopvalue and also the firstderivative of the detector signal first decreases and then increasesabove the negative of the Slope Stop value If only one of the stopsignal conditions is fulfilled the fraction collection will not stop

Start L+S End L or S - The fraction collection

starts when both start signal conditions are fulfilled simultaneouslydetector signal increases above Level Start value and also the firstderivative of the detector signal increases above Slope Start value Ifonly one of the start signal conditions is fulfilled the fraction collectionwill not startstops when any of the stop signal conditions is fulfilled (whateveroccurs first) detector signal decreases below Level Stop value or thefirst derivative of the detector signal first decreases and then increasesabove the negative of the Slope Stop value If none of the stop signalconditions is fulfilled the fraction collection will not stop before StopTime is reached

Start L or S End L+S - The fraction collection

starts when any of the start signal conditions is fulfilled (whateveroccurs first) detector signal increases above Level Start value or thefirst derivative of the detector signal increases above Slope Startvalue If none of the start signal conditions is fulfilled the fractioncollection does not startstops when both stop signal conditions are fulfilled simultaneouslydetector signal decreases below Level Stopvalue and also the firstderivative of the detector signal first decreases and then increasesabove the negative of the Slope Stop value If only one of the stopsignal conditions is fulfilled the fraction collection does not stop

Start L or S End L or S - The fraction collection

starts when any of the start signal conditions is fulfilled (whateveroccurs first) detector signal increases above Level Start value or thefirst derivative of the detector signal increases above Slope Start

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Gilson 203B204 4 Using the controlmodule

value If none of the start signal conditions is fulfilled the fractioncollection does not startstops when any of the stop signal conditions is fulfilled (whateveroccurs first) detector signal decreases below Level Stop value or thefirst derivative of the detector signal first decreases and then increasesabove the negative of the Slope Stop value If none of the stop signalconditions is fulfilled the fraction collection does not stop before StopTime is reached

FilterSets the data filter on the detector signal defined on the given row ofthe fraction table Default value of 1 means no filtering of data will beperformed value of X means each X data points received will beaveraged to 1 point used for fraction startend conditions detectionThe reaction to the fulfilled startstop signal condition will be delayedthe higher the Filter value is set mainly on detectors with low datarate

Note Filter settings influence the value that should be set the Slope Start andSlope Stop columns To get the correct trigger value for the Slope Start orSlope Stop using the Show SlopeLevel tool in the Chromatogramwindow it is necessary to set the Global Bunching value in theIntegration Table to the same value as the desired filter

Note The values coming from Detector Signal are evaluated in bunchapproximately every 300 milliseconds with respect to Start of Stop valuesinserted in Fraction Table It means that the positions of fraction collectionStart and Stop events in consecutive chromatograms with exactly thesame signal curve can differ plusmn 300 milliseconds In case of usage of thedetector with high sampling rate (for example 50 Hz) the difference offraction collection events between two consecutive chromatograms withexactly the same signal curve can be up to plusmn 15 datapoints (50 Hz is 50datapoints per second thus15 datapoints per 300milliseconds)

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Gilson 203B204 4 Using the controlmodule

Note It is strongly recommended not to use Slope Signal Condition for fractioncollection based on noisy detector signal

Start L+S End L+S - The fraction collection

starts when both start signal conditions are fulfilled simultaneouslydetector signal increases above Level Start value and also the firstderivative of the detector signal increases above Slope Start value Ifonly one of the start signal conditions is fulfilled the fraction collectionwill not startstops when both stop signal conditions are fulfilled simultaneouslydetector signal decreases below Level Stopvalue and also the firstderivative of the detector signal first decreases and then increasesabove the negative of the Slope Stop value If only one of the stopsignal conditions is fulfilled the fraction collection will not stop

Start L+S End L or S - The fraction collection

starts when both start signal conditions are fulfilled simultaneouslydetector signal increases above Level Start value and also the firstderivative of the detector signal increases above Slope Start value Ifonly one of the start signal conditions is fulfilled the fraction collectionwill not startstops when any of the stop signal conditions is fulfilled (whateveroccurs first) detector signal decreases below Level Stop value or thefirst derivative of the detector signal first decreases and then increasesabove the negative of the Slope Stop value If none of the stop signalconditions is fulfilled the fraction collection will not stop before StopTime is reached

Start L or S End L+S - The fraction collection

starts when any of the start signal conditions is fulfilled (whateveroccurs first) detector signal increases above Level Start value or thefirst derivative of the detector signal increases above Slope Startvalue If none of the start signal conditions is fulfilled the fractioncollection does not startstops when both stop signal conditions are fulfilled simultaneouslydetector signal decreases below Level Stopvalue and also the firstderivative of the detector signal first decreases and then increasesabove the negative of the Slope Stop value If only one of the stopsignal conditions is fulfilled the fraction collection does not stop

Start L or S End L or S - The fraction collection

starts when any of the start signal conditions is fulfilled (whateveroccurs first) detector signal increases above Level Start value or thefirst derivative of the detector signal increases above Slope Start

- 10 -

Gilson 203B204 4 Using the controlmodule

value If none of the start signal conditions is fulfilled the fractioncollection does not startstops when any of the stop signal conditions is fulfilled (whateveroccurs first) detector signal decreases below Level Stop value or thefirst derivative of the detector signal first decreases and then increasesabove the negative of the Slope Stop value If none of the stop signalconditions is fulfilled the fraction collection does not stop before StopTime is reached

FilterSets the data filter on the detector signal defined on the given row ofthe fraction table Default value of 1 means no filtering of data will beperformed value of X means each X data points received will beaveraged to 1 point used for fraction startend conditions detectionThe reaction to the fulfilled startstop signal condition will be delayedthe higher the Filter value is set mainly on detectors with low datarate

Note Filter settings influence the value that should be set the Slope Start andSlope Stop columns To get the correct trigger value for the Slope Start orSlope Stop using the Show SlopeLevel tool in the Chromatogramwindow it is necessary to set the Global Bunching value in theIntegration Table to the same value as the desired filter

Note The values coming from Detector Signal are evaluated in bunchapproximately every 300 milliseconds with respect to Start of Stop valuesinserted in Fraction Table It means that the positions of fraction collectionStart and Stop events in consecutive chromatograms with exactly thesame signal curve can differ plusmn 300 milliseconds In case of usage of thedetector with high sampling rate (for example 50 Hz) the difference offraction collection events between two consecutive chromatograms withexactly the same signal curve can be up to plusmn 15 datapoints (50 Hz is 50datapoints per second thus15 datapoints per 300milliseconds)

- 11 -

Gilson 203B204 4 Using the controlmodule

value If none of the start signal conditions is fulfilled the fractioncollection does not startstops when any of the stop signal conditions is fulfilled (whateveroccurs first) detector signal decreases below Level Stop value or thefirst derivative of the detector signal first decreases and then increasesabove the negative of the Slope Stop value If none of the stop signalconditions is fulfilled the fraction collection does not stop before StopTime is reached

FilterSets the data filter on the detector signal defined on the given row ofthe fraction table Default value of 1 means no filtering of data will beperformed value of X means each X data points received will beaveraged to 1 point used for fraction startend conditions detectionThe reaction to the fulfilled startstop signal condition will be delayedthe higher the Filter value is set mainly on detectors with low datarate

Note Filter settings influence the value that should be set the Slope Start andSlope Stop columns To get the correct trigger value for the Slope Start orSlope Stop using the Show SlopeLevel tool in the Chromatogramwindow it is necessary to set the Global Bunching value in theIntegration Table to the same value as the desired filter

Note The values coming from Detector Signal are evaluated in bunchapproximately every 300 milliseconds with respect to Start of Stop valuesinserted in Fraction Table It means that the positions of fraction collectionStart and Stop events in consecutive chromatograms with exactly thesame signal curve can differ plusmn 300 milliseconds In case of usage of thedetector with high sampling rate (for example 50 Hz) the difference offraction collection events between two consecutive chromatograms withexactly the same signal curve can be up to plusmn 15 datapoints (50 Hz is 50datapoints per second thus15 datapoints per 300milliseconds)

- 11 -

Gilson 203B204 4 Using the controlmodule

42 Method Setup - FC - Vial NumbersThe Method Setup - FC - Vial Numbers tab is used for setting the behaviorof the Gilson 203B204 fraction collector

Fig 6 Method Setup - FC - VialNumbers

Start Vial NumberVial NumberSets the number of the vial where the first collected fraction will be storedThe current vial number is incremented each time the Next signal isactivated The vial number is stored in the Results TableReset atSpecifies the conditions of the current vial number setting to Start VialNumber in three different situations

Each Run - the vial number is reset at the start of each acquisitionStart Sequence - the vial number is reset only at the start of asequenceOpen Instrument - the vial number is reset only once when theInstrument is opened

Last Vial NumberVial NumberSets the number of the last available vial

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Gilson 203B204 4 Using the controlmodule

After Last VialSpecifies the action executed when current vial number exceeds the LastVial Number

Waste - the acquisition continues but the fraction table is ignored andthe samples are automatically discardedShutdown - the acquisition is immediately stopped and the analysisaborted

Vial Change OptionsWaste During Vial ChangeThis option prevents spilling when the vial is changed during collection Ittemporary stops the fraction collection before changing the vial andrestarts it again when the vial is swapped The vial is changed in themiddle of the wasting interval the volume wasted during vial change canbe specified in the Volume field Volume units are the ones set in theInstrument Method Sending dialog This functionality is not applicable(even if checked) when Collect All option is selectedChange Vial when Collecting and Peak Top is DetectedIf enabled the vial is automatically swapped when the peak top isdetected during collection Be aware this option is not applied if the activerow in the Fraction Table is set to None in the Signal Condition columnCollect AllWhen checked fractions are collected during the whole course of theanalysis regardless of the settings in the Fraction Table The FC modulethen sends the Next Vial command only which makes this mode ofoperation suitable for a fraction collector without a waste position CollectAll mode does not permitWaste During Vial ChangeThe maximum amount of eluent collected in a single vial is limited by thevalue in Vial Volume field Once this volume is filled it begins collectinginto the next successive vial

Note With Collect All option the valve is not controlled

Warning Once error message Vial number exceeded last vial number isdisplayed it means that the FC was already at the last position whencommand Next Vial was received Collecting stops at position which isequal to the Last Vial Number - 1 because its the last possible positionto collect (due toWaste) Correct appropriately your method

- 13 -

Gilson 203B204 4 Using the controlmodule

After Last VialSpecifies the action executed when current vial number exceeds the LastVial Number

Waste - the acquisition continues but the fraction table is ignored andthe samples are automatically discardedShutdown - the acquisition is immediately stopped and the analysisaborted

Vial Change OptionsWaste During Vial ChangeThis option prevents spilling when the vial is changed during collection Ittemporary stops the fraction collection before changing the vial andrestarts it again when the vial is swapped The vial is changed in themiddle of the wasting interval the volume wasted during vial change canbe specified in the Volume field Volume units are the ones set in theInstrument Method Sending dialog This functionality is not applicable(even if checked) when Collect All option is selectedChange Vial when Collecting and Peak Top is DetectedIf enabled the vial is automatically swapped when the peak top isdetected during collection Be aware this option is not applied if the activerow in the Fraction Table is set to None in the Signal Condition columnCollect AllWhen checked fractions are collected during the whole course of theanalysis regardless of the settings in the Fraction Table The FC modulethen sends the Next Vial command only which makes this mode ofoperation suitable for a fraction collector without a waste position CollectAll mode does not permitWaste During Vial ChangeThe maximum amount of eluent collected in a single vial is limited by thevalue in Vial Volume field Once this volume is filled it begins collectinginto the next successive vial

Note With Collect All option the valve is not controlled

Warning Once error message Vial number exceeded last vial number isdisplayed it means that the FC was already at the last position whencommand Next Vial was received Collecting stops at position which isequal to the Last Vial Number - 1 because its the last possible positionto collect (due toWaste) Correct appropriately your method

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Gilson 203B204 4 Using the controlmodule

43 Hardware ConfigurationThe Hardware Configuration dialog (invoked by using the FC Statusbutton from the Method Setup - LC Method Setup - FC Method Setup -Valves dialog) displays the configuration of the Gilson 203B204 namelythe communication type and its parameters

Fig 7 Gilson 203BHardware Configuration

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Gilson 203B204 4 Using the controlmodule

44 Device MonitorThe window with the fraction collector status can be invoked by theMonitor - Device Monitor command from the Instrument window or usingthe Device Monitor icon

Device Monitor - Fraction Collector

Fig 8 DeviceMonitor - Gilson 203BFraction Collector

Current PositionDisplays whether the fraction collector is currently wasting or the positionto which it is currently collectingNextMoves the collection vessel one vial forward Using this button while thecollector is performing its program set on the Method Setup - FC - FractionTable tab during an analysis run immediately overrides the automaticcollection program To return to the automatic program use the ResumeMethod buttonCollect WasteThe description on the button changes according to the state in which thefraction collector is - while it is collecting the Waste button forces thefraction collector to stop collecting while it is in a waste cycle the Collectbutton will force the collector to start collecting Using any of the twoactions while the collector is performing the program set on the MethodSetup - FC - Fraction Table tab during an analysis run immediatelyoverrides the automatic collection program To return to the automaticprogram use the Resume Method buttonPosition To GoAllows to set the position to which the fraction collector should beswitched The switching itself is performed using the Go To PositionbuttonResume MethodResumes the actions of the automatic fraction table set on the MethodSetup - FC - Fraction Table tab If the fraction collector is collecting at that

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Gilson 203B204 4 Using the controlmodule

44 Device MonitorThe window with the fraction collector status can be invoked by theMonitor - Device Monitor command from the Instrument window or usingthe Device Monitor icon

Device Monitor - Fraction Collector

Fig 8 DeviceMonitor - Gilson 203BFraction Collector

Current PositionDisplays whether the fraction collector is currently wasting or the positionto which it is currently collectingNextMoves the collection vessel one vial forward Using this button while thecollector is performing its program set on the Method Setup - FC - FractionTable tab during an analysis run immediately overrides the automaticcollection program To return to the automatic program use the ResumeMethod buttonCollect WasteThe description on the button changes according to the state in which thefraction collector is - while it is collecting the Waste button forces thefraction collector to stop collecting while it is in a waste cycle the Collectbutton will force the collector to start collecting Using any of the twoactions while the collector is performing the program set on the MethodSetup - FC - Fraction Table tab during an analysis run immediatelyoverrides the automatic collection program To return to the automaticprogram use the Resume Method buttonPosition To GoAllows to set the position to which the fraction collector should beswitched The switching itself is performed using the Go To PositionbuttonResume MethodResumes the actions of the automatic fraction table set on the MethodSetup - FC - Fraction Table tab If the fraction collector is collecting at that

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Gilson 203B204 4 Using the controlmodule

moment it will start a waste cycle and then resume the function of thefraction table

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Gilson 203B204 4 Using the controlmodule

45 Data Acquisition window

Fig 9 Data Acquisition window

The Data Acquisition window can display the fractions in the graph usingthe background color and also the start and stop fraction events andmarker of the Fraction Delay To see the fractions and markers right clickon the graph select Propertieshellip to open the Graph Properties dialog andcheck the Show Events checkboxWhen the Fraction Delay is set there will be two colors present in thegraph - lighter and darker red The lighter red color shows the expectedduration of the fraction (which Clarity only presumes based on data pointsit got so far) while the darker red color shows the placement of the realfractions

Note The Fraction Delay in the picture will appear if the Delay Volume field intheMethod Setup - FC - Fraction Table dialog is set to a nonzero value

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Gilson 203B204 4 Using the controlmodule

45 Data Acquisition window

Fig 9 Data Acquisition window

The Data Acquisition window can display the fractions in the graph usingthe background color and also the start and stop fraction events andmarker of the Fraction Delay To see the fractions and markers right clickon the graph select Propertieshellip to open the Graph Properties dialog andcheck the Show Events checkboxWhen the Fraction Delay is set there will be two colors present in thegraph - lighter and darker red The lighter red color shows the expectedduration of the fraction (which Clarity only presumes based on data pointsit got so far) while the darker red color shows the placement of the realfractions

Note The Fraction Delay in the picture will appear if the Delay Volume field intheMethod Setup - FC - Fraction Table dialog is set to a nonzero value

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Gilson 203B204 4 Using the controlmodule

46 DataApex UNI SetupThe appearance of the DataApex UNI Setup dialog depends on thepresence of the selected Ruby Script - if the script is not present only theRuby Script field is visible

Fig 10 DataApexUNI Setup

Ruby ScriptDisplays the selected Ruby Script The correct GILSON203BFCRB scriptfor the Gilson 203B204 instrument can be found in the UTILSUNI_DRIVERSGILSON subdirectory (accessible through the button) ofthe Clarity installation folder (CCLARITYBIN by default)PortDefines the communication port used possible values dependent on thetype of communication of the device andor available ports in the PCAutoDetectIt is used for verifying the device communication over the serial portselected aboveInstrument NameAllows you to set the custom name of the instrument This name (enteredinto the Value column) will be used throughout the Clarity stationDevice TypeDisplays the subtype of the fraction collectorBaud RateSets the Baud Rate communication parameter for the Gilson 203B204fraction collector Available values are 4800 9600 and 19200 baudPlease note that the value set in the DataApex UNI Setup dialog must bethe same as the one set on the fraction collector

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Gilson 203B204 4 Using the controlmodule

GSIOC IDSets or shows (if autodetected) the ID of the fraction collector on theGSIOC protocolQuick ShutdownIf checked quick shutdown procedure is applied when the instrumentwindow is closed When an immediate opening of the just closedinstrument is then desired a communication error window may appearIf unchecked the shutdown of the Instrument window takes approximately10 seconds thus preventing a communication error window appearanceafter an immediate opening of the just closed instrument is then desired

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Gilson 203B204 5 Report Setup

5 Report SetupThe fraction collector section on the method report can be enabled bychecking the Instrument Control checkbox on the Method tab of the ReportSetup dialog

Fig 11 Report Setup

All of the parameters set in the Method Setup - FC - Fraction Table andMethod Setup - FC - Vial Numbers tabs are reported as well as thecustom FC Name and other parameters set in the DataApex UNI Setupdialog

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Gilson 203B204 4 Using the controlmodule

GSIOC IDSets or shows (if autodetected) the ID of the fraction collector on theGSIOC protocolQuick ShutdownIf checked quick shutdown procedure is applied when the instrumentwindow is closed When an immediate opening of the just closedinstrument is then desired a communication error window may appearIf unchecked the shutdown of the Instrument window takes approximately10 seconds thus preventing a communication error window appearanceafter an immediate opening of the just closed instrument is then desired

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Gilson 203B204 5 Report Setup

5 Report SetupThe fraction collector section on the method report can be enabled bychecking the Instrument Control checkbox on the Method tab of the ReportSetup dialog

Fig 11 Report Setup

All of the parameters set in the Method Setup - FC - Fraction Table andMethod Setup - FC - Vial Numbers tabs are reported as well as thecustom FC Name and other parameters set in the DataApex UNI Setupdialog

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Gilson 203B204 5 Report Setup

5 Report SetupThe fraction collector section on the method report can be enabled bychecking the Instrument Control checkbox on the Method tab of the ReportSetup dialog

Fig 11 Report Setup

All of the parameters set in the Method Setup - FC - Fraction Table andMethod Setup - FC - Vial Numbers tabs are reported as well as thecustom FC Name and other parameters set in the DataApex UNI Setupdialog

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Gilson 203B204 6 Troubleshooting

6 TroubleshootingWhen the solution to a problem cannot be found easily a recording of thecommunication between Clarity and the fraction collector will significantlyhelp DataApex supportThe data recording can be enabled by adding or amending theCOMMDRVINI file in the Clarity installation directory (CCLARITYCFGby default) The file can be edited in any text editor (eg Notepad) Thefollowing section should be edited or added

[COM1]echo=ontextmode=onfilename=CommDrvCOM1_Dtxtreset=off

Note Instead of COM1 type the communication port used to communicate withthe Gilson 203B204 fraction collector This port number is displayedwhen the FC Statusbutton in theMethod Setup - FC dialog is invoked

Note D (or d) in the filename parameter means that the log will be createdseparately for each day The reset=off parameter disables deleting thecontent of the log each time the station is started during the same day

The created TXT files will be of great help in the diagnosis of notdocumented errors and communication issues

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• 1 Gilson 203B204 Control Module
• 2 Requirements
• 3 Installation Procedure
• • 31 Gilson 203B204 fraction collector communication
  • 32 Clarity Configuration
  • • 4 Using the control module
    • • 41 Method Setup - FC - Fraction Table
      • 42 Method Setup - FC - Vial Numbers
      • 43 Hardware Configuration
      • 44 Device Monitor
      • 45 Data Acquisition window
      • 46 DataApex UNI Setup
      • • 5 Report Setup
        • 6 Troubleshooting