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Customized solutions from TIG (Tecan Integration Group) Genomics: Molecular Biology Workstation Cloning, Protein Expression and Purification Molecular Biology workstation for 11 different assay types Technical Reference 30013695 Process 11 different assay types PCR set up and PCR (2 types) Gateway cloning (2 types) Transformation of E.coli Restriction digest Plasmid extraction (Macherey-Nagel® NucleoSpin® Robot 96 Plasmid Kit) Clean up of PCR products (Qiagen® QIAquick® 96 PCR Kit) Loading of running E-GEL® 96 (Invitrogen®) Purification of proteins using Promega® magnetic GST Agarose Beads incl. Bradford with BSA standard curve Purification of proteins using Promega Ni-NTA Superflow 96 Robot Kit incl. Bradford with BSA standard curve Input Plates 96 well PCR MJ Research® plates 96 well Corning® plates Deep well plates Filter plates Tubes Eppendorf ® 1.5 ml tubes Output Throughput One plate a day per purification Several cloning and expression assays can be run additionally.

Genomics: Molecular Biology Workstation Cloning, Protein

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Page 1: Genomics: Molecular Biology Workstation Cloning, Protein

Customized solutions from TIG(Tecan Integration Group)

Genomics: Molecular Biology Workstation

Cloning, Protein Expression and PurificationMolecular Biology workstation for 11 different assay types

Technical Reference 30013695

Process

11 different assay types

PCR set up and PCR (2 types) Gateway cloning (2 types) Transformation of E.coli Restriction digest Plasmid extraction (Macherey-Nagel®

NucleoSpin® Robot 96 Plasmid Kit) Clean up of PCR products (Qiagen®

QIAquick® 96 PCR Kit) Loading of running E-GEL® 96

(Invitrogen®) Purification of proteins using

Promega® magnetic GST AgaroseBeads incl. Bradford with BSAstandard curve

Purification of proteins using PromegaNi-NTA Superflow 96 Robot Kit incl.Bradford with BSA standard curve

Input

Plates

96 well PCR MJ Research®plates

96 well Corning® plates Deep well plates Filter plates

Tubes

Eppendorf® 1.5 ml tubes

Output

Throughput

One plate a day per purification

Several cloning and expressionassays can be run additionally.

Page 2: Genomics: Molecular Biology Workstation Cloning, Protein

Customized solutions from TIG(Tecan Integration Group)

Manufactured by TIG (Tecan Integration Group), Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland,Email [email protected], www.tecan.com, p 2/4

Highlights

Cooling hotel in the PosID™ area, E-Gel 96 and Qiagen magnet on microplate carrier, H+P Thermoshake in PosID area

H+P Thermoshake, left module with adapter for filter plate

Page 3: Genomics: Molecular Biology Workstation Cloning, Protein

Customized solutions from TIG(Tecan Integration Group)

Manufactured by TIG (Tecan Integration Group), Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland,Email [email protected], www.tecan.com, p 3/4

Modified Te-VacS™ with DiTi Waste chute to save space on the deck

Integrated E-GEL 96 Modified trough carrier 200 ml to safe space on deck

Page 4: Genomics: Molecular Biology Workstation Cloning, Protein

Customized solutions from TIG(Tecan Integration Group)

Manufactured by TIG (Tecan Integration Group), Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland,Email [email protected], www.tecan.com, p 4/4

Integration of two MJ Research® Thermocyclers

GENios™ Reader Stand alone DiTi racks stored in hotel

Scientific instrumentation; not for use in human, clinical or diagnostic procedure. All systems or products are tailor-made one-offs that were exclusively developed oncustomer demand. All displayed pictures and images originate from development-, test- or installation phases and do not claim to represent the final installation atcustomer site.

Tecan and Freedom EVO are in major countries registered trademarks and GENios, Te-VacS, PosID are trademark of Tecan Group Ltd., Männedorf, Switzerland.Eppendorf is a registered trademark of Eppendorf-Netheler-Hinz GmbH, Hamburg, Germany. QIAGEN is a registered trademark of QIAGEN GmbH Corp., Hilden,Germany. MJ Research is a registered trademark of Bio-Rad Laboratories, Inc., Hercules USA. Invitrogen and E-Gel 96 are registered trademarks of InvitrogenCorporation, Carlsbad, USA . MACHEREY -NAGEL and NucleoSpin are registered trademarks of Macherey-Nagel, Düren, Germany. Promega is a registeredtrademark of Promega Corporation, Madison, USA . H+P a unit of Thermo Electron Corporation. Corning is a registered trademark of Corning Incorporated, Corning,USA. QIAquick is a registered trademark of QIAGEN GmbH Corp., Hilden, Germany.

© 2008, Tecan Trading AG, Switzerland, all rights reserved.