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8/25/17
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BIOS10191Biology’sImpactinOurWorld
Instructors
Dr.JoeO’Tousa Dr.GaryBelovsky
Week1 Ques@onnaire-Week1
• 9.Inmyopinion,thebestreasonforstudyingbiologyis:
• ________________________________________________________________________• ________________________________________________________________________• ________________________________________________________________________•
• 10.ThemostconfusingtopicinbiologythatIhaveeverhadtostudyis:•
________________________________________________________________________• ________________________________________________________________________• ________________________________________________________________________
Ge<ngstarted Ge<ngstarted
Ge<ngstarted• edX,SakaiSite• Syllabus• Weeklyclassformat(onetopicperweek)
ClassDay-1(Monday)2(Wednesday)3(Friday)
InClass--Lecture,quizLecture,in-classworksheets,quizOther:labexerciseDebates,studentvideos
OutofClassPre-lectureedXworksheet(A),reflec@onPre-lectureedXworksheet(B),reflec@onOthertypeofassignment
Ge_ngstartedGrading
Gradesarebasedontheclassa`endance,par@cipa@on,classquizzes,andfinalexam:
1.A`endance 20%2.Prelectureassignments,essays 20%3.ClassDiscussion, Presenta@ons,Quizzes 20%4.MidtermExam(Fri,Oct.13) 20%5.FinalExam(Friday,Dec.15): 20%
AllgradedassignmentscoreswillbepostedontheSAKAIsiteonaweeklybasis.
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Ge<ngstartedFORFRIDAYedX:week1. Week1• DNAisthemoleculecontainingthegene@cinforma@on.• TherulesofbasepairingmakesiteasytounderstandhowaDNA
moleculecanbereplicated.• Celltheory,history,@metodevelop->1665-->1830.• Cellmembranes.• DNA/gene@cmaterialmust:
(1)replicate. (2)makeproductsforcelltowork.
• ThecellularmachineryresponsibleforDNAreplica@oncanbeharnessedtocreateausefuldiagnos@ctoolcalledthepolymerasechainreac@on(PCRforshort).
• ReviewtheWatson/Crick/RosalindFranklinHistory
| | | | | | | | | | | | | |
Q#1.TwostrandsmakeaDNAmolecule:
T-A-G-G-C-G-A-T-T-A-G-G-C-G
A-T-C-C-G-C-T-A-A-T-C-C-G-C
C-G-G-T-A-C-A-A-T-T-G-A-G-GWhatissequenceoflowerstrand?
DNAisthemoleculecontainingthegeneAcinformaAon.
TheNucleo@desofDNA
ThebuildingblocksforDNAaretheA,C,[email protected],asshowninstructureatright,sodifferonlybythe“Base”.Eachnucleo@deisnamedforthebaseA,C,G,orT.
A C G TA A A A A
A A A A
DNAisapolymercomposedofnucleo@des
DNAhasapolarityknownas5’à3’
backbone
base
A C G TA A A A A
A A A A
DNAisapolymercomposedofnucleo@des
DNAhasapolarityknownas5’à3’
backbone
base
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CellTheory1665:RobertHookediscoveredamul@tudeof@nyporesincorkthathenamed"cells".
“Cell”--fromtheLa@nwordCella,meaning‘asmallroom’likemonkslivedinandalsoCellulae,whichmeantthesixsidedcellofahoneycomb.
1.Alllivingorganismsarecomposedofoneormorecells.
2.Thecellisthemostbasicunitoflife.3.Allcellsariseonlyfrompre-exis@ngcells.(1852)
CellTheoryIn1839:plantsandanimalsarecomposedofcellsortheproductofcells.
Acellmembrane Acelldivision
Eachofthetwoprogenycells(daughtercells)needstohavethesamecontentofDNAasparentcell.
1. DNAreplica@onoccursina5’à3’direc@on
2. DNAneedsaprimertoini@atereplica@on.
| | | | | | | | | | | | | |
TheDNAstrandsruninoppositedirec@ons::
3’ T-A-G-G-C-G-A-T-T-A-G-G-C-G 5’
5’ A-T-C-C-G-C-T-A-A-T-C-C-G-C 3’
5’ C-G-G-T-A-C-A-A-T-T-G-A-G-G 3’
A.or
B.
Q2:DNAcanonlybemadeina5’to3’[email protected]@onofthenewsynthesis:
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BIOS10191Biology’sImpactinOurWorld
StartàFriday,Week1DNAisthemoleculecontainingthegene@cinforma@on.TherulesofbasepairingmakesiteasytounderstandhowaDNAmoleculecanbereplicated.Celltheory,history,@metodevelop->1665-->1830.Cellmembranes.DNA/gene@cmaterialmust:
(1)replicate. (2)makeproductsforcelltowork.
ThecellularmachineryresponsibleforDNAreplica@oncanbeharnessedtocreateausefuldiagnos@ctoolcalledthepolymerasechainreac@on(PCRforshort).SequenceDNA.
Ques@onnaire-firstday• 10.ThemostconfusingtopicinbiologythatIhaveeverhadtostudyis:• ________________________________________________________________________• ________________________________________________________________________• ________________________________________________________________________
Cellularenergy,KrebsCycle(mitochondria),Photosynthesis,Kelvincycle(chloroplasts)DNAreplica@on,DNAstructureGene@cs,mitosis,meiosis,inheritancera@osNeurobiology—ClassifyingorganismsEcology—Plants—phospholipids—Noanswer(“tookBiomyfirstyearofHS”)
9
8
4
1-1-1-1
2-2
h`ps://youtu.be/5qSrmeiWsuc?t=4m14s
Start4:14,runto6:30.
DNAReplicaAon:TheCell'sExtremeTeamSportPolymeraseChainReac@on(Part1)
SHOWANIMATIONINCLASS
SHOWANIMATIONINCLASS
PolymeraseChainReac@on(Part2)
CopiesofT
argetD
NA
124
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PolymeraseChainReac@on(Part3)
30cyclestakesabout3hrsandprovidesmorethanamillioncopiesofDNAtarget.
Introduc@ontoProteins:the“skilledlabor”ofthecell
• makingproteinsfromgenes• proteinfolding,proteinstructure• proteinstructurespecifiesfunc@on• muta@onsinDNAmayalterproteinfolding,func@on
UsingGene@cInforma@ontomakeaProtein
“THECENTRALDOGMA”
protein
DNA→
RNA→
Protein
6/08/2017
DNAtoProtein
3.2.
1.
4.
Q1.Fillintheblankswithanappropriateterm.
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Worksheet:makeaprotein!
…GCGCATTTAGCATGCTTATGCATTGGCAGCCCTACTAGCGGGCAAT……CGCGTAAATCGTACGAATACGTAACCGTCGGGATGATCGCCCGTTA…
transcrip@onstart
transla@onstart
Q1.Whatisthenameoftheaminoacidthatwillbethefourthaminoacidoftheprotein?
AProteinisaPolymertoo…
backbone
sidechains
Foldedandac@veprotein
X-rayanalysisonlysozyme
Whydoproteinsfoldinapar@cularway?
Aminoacidsidechains:differenta`rac@ve/repulsiveforcesexis@ngbetweentheindividualaminoacidsallowpar@cularfoldedproteinstobestable JaneRichardson
h`ps://www.youtube.com/watch?v=uqQlwYv8VQI
s@ckandballviewofprotein Ribbonviewofprotein
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AProteininAc@on
Compound2
Compound1
6/08/2017
GeneMuta@ons
Gene@cDiseaseduetoThrombinmuta@on
6/08/2017
Q2.Ifthispartoftheproteinmadebythean@thrombingenehastheaminoacidsequence:
-GLY-ASP-LEU-SER-
…howwillthenucleo@dechangefoundinthegene@cdiseaseversionofthegenealtertheprotein?
GGCGACGTGAGCGeneOcdiseasesequence
GGCGACCTGAGC
Wrapping Up……Next Week: DNA in the Courtroom
→CompletetheedXWeek2PartAassignmentbeforeMonday’sClass→Rememberoneweekfromtoday(Friday9/1)classheldattheDNALearningCenterinJordanHall
