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Genetics News
Mystery sequence project
Lost book
Problem Day Friday
Unknown Day Monday
Where to get help before the exam
Wednesday, Sept 20 6:30 PM - 8:00 PM E-107 Sarah Muller
Wednesday, Sept 20 7:00 PM - 9:00 PM UFA2107 Jennifer Lee
Thursday, Sept 21 4:00 PM - 5:00 PM S-215 Garrett Griffin
Friday, Sept 22 3:00 PM - 5:00 PM S-215 Jeff Elhai
Sunday, Sept 24 8:00 PM - 10:00 PM E-209 Karen Lewis
Monday, Sept 25 2:30 PM - 3:30 PM S-111 Sarah Muller
Monday, Sept 25 5:15 PM - 6:15 PM S-111 Sarah Muller
See Who We Are page on web.Click on Special Help Sessions.
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
substrate
Pathways: Making Autos
substrateconsumed
Product
Pathways: Making Pigment
chemical A(vermilion color)
Enzymes
Cell
Pathways: Making Pigment
Modify chemical A
Enzyme X
Pathways: Making Pigment
Enzyme X
chemical B(cinnabar color)
Pathways: Making Pigment
Enzyme Y
Modify chemical B
Pathways: Making Pigment
Wild-type pigment(red color)
Enzyme Y
Pathways: Making Pigment
Accumulate wild-type pigment
X+ Y+
X+ Y-
X- Y+
Wild-type eye color
Cinnabar eye color
Vermilion eye color
When Pathways Go Wrong
X+ Y-
X- Y+
When Pathways Go Wrong
Imaginal disk tissue
Abdominal tissue
X+ Y-
When Pathways Go Wrong
Imaginal disk tissue
Abdominal tissue
Wild-type eye color
X- Y+
Pulse-Chase ExperimentDoes RNA move from nucleus to cytoplasm?
Pre pulse
UU
UU
U
UU
U
UU
UUU
U
RNA
Uracil
Pulse-Chase ExperimentDoes RNA move from nucleus to cytoplasm?
Pulse
UU
UU U
UU
U
RNA
Uracil
UU
U
UUUU
U
Pulse-Chase ExperimentDoes RNA move from nucleus to cytoplasm?
Chase
UU
UU
U
UU
U
UU
UUU
U
RNA
Uracil
Comparison of artificial RNA polymer experiment & amino acid swap experiment
Artificial polymer Amino acid swap
Need ribosomes, tRNA, tRNA synthetases Cell extract Cell extract
Need to focus on one amino acid
Label each aa, one at a time Label cysteine
Need artificial polymer Use poly(U,G) Use poly(U,G)
Isolate new protein Precipitate, filter Precipitate, filter
Experimental need
Comparison of artificial RNA polymer experiment & amino acid swap experiment
Artificial polymer Amino acid swap
Need ribosomes, tRNA, tRNA synthetases Cell extract Cell extract
Need to focus on one amino acid
Label each aa, one at a time Label cysteine
Need artificial polymer Use poly(U,G) Use poly(U,G)
Isolate new protein Precipitate, filter Precipitate, filter
Experimental need
Alter amino acid on tRNA ---
Convert cysteine to alanine
Classes of RNA and Why they are
Table 1. Comparison of classes of RNA in a typical bacterium (E. coli)Class Types Fraction Sizes Stability Function
Ribosomal RNA(rRNA)
3 80%2904bp,
1542bp, 120bpstable
Scaffold for ribosomal proteins,participates in translation
Transfer RNA(tRNA)
37 12% 76bp to 91 bp stable Connects codon to amino acid
Messenger RNA(mRNA)
1000’s 5%~300 bp to~10,000 bp
unstableCarries information on amino acidsequence of protein
