Upload
sudhakaranr
View
222
Download
0
Embed Size (px)
Citation preview
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 1/26
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 2/26
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 3/26
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 4/26
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 5/26
WATER DEFICIT is acritical environmentalfactors that limitagricultural production
worldwide. WATER DEFICIT
causes a series of morphological,physiological,
biochemical, andmolecular changes inplants.
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 6/26
Water deficit resistance-
Plants
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 7/26
Water deficit tolerance
in
transgenic
Arabidopsis thaliana
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 8/26
SNF1-related protein kinase 2 (SnRK2), SRK2C,
is an osmotic-stress-activated protein kinase in
Arabidopsis thaliana that can significantly impact
water deficit tolerance of Arabidopsis plants.
Knockout mutants of SRK2C exhibited water
deficit hypersensitivity in their roots, suggestingthat SRK2C is a positive regulator of water
deficit tolerance in Arabidopsis roots.
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 9/26
Materials and Methods
1. Generation of TransgenicPlants or Cells.
The coding region of SRK2CcDNA was amplified by RT-PCR
by using primers ( X
baIsite) and(BamHI site) and cloned into
pBE2113 vector using CaMV35S promoter.
Arabidopsis thaliana culturedcells were transformed by using Agrobacterium tume-faciens.
2.Cell Culture, Plant Growth,and Stress Treatments.
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 10/26
Cont,
3. Gel Kinase Assay.
4.Immunocomplex Kinase Assay
5.RNA Gel Blot Analysis.
6.Microarray Analysis and Data Mining.
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 11/26
RESULTS
Transgenic plants displayed higher overall water deficit tolerance than control plants.
Microarray analysis revealed that their water deficit tolerancecoincided with up-regulation of many stress-
responsive genes, for example, RD29A, COR15A, and
DREB1A/CBF3.
Results,concluded that SRK2C is capable of mediating signals
initiated during water deficit stress, resulting in appropriate
gene expression.
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 12/26
Transformation of tomato cultivar
`Pusa Ruby' with bspA gene from
Populus tremula for water deficit tolerance
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 13/26
bspA
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 14/26
Transgenic wheat
DREB1A
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 15/26
Result
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 16/26
Lily ASR Protein -Conferswater deficit tolerance in
Arabidopsis
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 17/26
LLA23
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 18/26
Result
LLA23 mediates stress-responsive ABA signaling.
In vegetative tissues, it is seen that transgenic plants
have a decreased rate of water loss and exhibitenhanced water deficit resistance.
LL A23-functions as water deficit resistant gene.
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 19/26
Effects of free proline accumulation
in petunias under water deficit
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 20/26
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 21/26
The transgenic plants accumulated Proline and their
drought tolerance was tested. The Proline content
amounted to 0.57±1.01% of the total amino acids in the
transgenic plants, under normal conditions.
The transgenic plant lines tolerated 14 days of drought
stress, which confirms that transgene had full
functionality.
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 22/26
Conclusions
W ater deficit-tolerance is acomplex phenomenon.
W hether transgenic plants will
ultimately contribute to the
struggle against world hunger,is a
Most test results are based on
greenhouse experiments.
There are no commercially
available varieties.
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 23/26
REFERENCES:
1-SRK2C, a SNF1-related protein kinase 2,
improves drought tolerance by controlling stress-
responsive gene expression in Arabidopsis
thalianaTaishi Umezawa , Riichiro Yoshida , Kyonoshin
Maruyama , Kazuko Yamaguchi-Shinozaki , and
Kazuo Shinozaki
PNAS | December 7, 2004 | vol. 101 | no. 49 |
17306-17311
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 24/26
2- Effects of free proline accumulation in petunias under water deficit stress
Mika Yamada, Hiromasa Morishita, Kaoru Urano, NorikoShiozaki, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozakiand Yoshu Yoshiba.
Journal of Experimental Botany 2005 56(417):1975-1981;
3- A Lily ASR Protein Involves Abscisic Acid Signaling and Confers Drought and Salt Resistance in Arabidopsis . .
Chin-Ying Yang, Yu-Chuan Chen, Guang Yuh Jauh andCo-Shine Wang.
Plant Physiology 139:836-846 (2005)
8/8/2019 Genetically Engineered Plants
http://slidepdf.com/reader/full/genetically-engineered-plants 25/26
4-Effects of free proline accumulation in
petunias under w
ater deficit.
Mika Yamada, Hiromasa Morishita, Kaoru Urano, NorikoShiozaki, Kazuko Yamaguchi-Shinozaki, Kazuo
Shinozaki and Yoshu Yoshiba,
Central Research Laboratory, c/o Advanced ResearchLaboratory, Hitachi Ltd., 2520 Akanuma, Hatoyama-cho,Hiki-gun, Saitama 350-0395, Japan
Research Resources Center, Brain Science Institute,RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan