Genetic Engineering

Genetic Engineering

aka .. Genetic Modification

Make changes in DNA code by Insert or delete specific genes

Use modern molecular biology techniques.

1) Recombinant DNA Aka .. Gene Splicing

made by combining DNA from > 1 source (often from very different species)

2) Gel electrophoresis An electric current smaller fragments -

fasterlarger ones – slower

DNA fragments move to

positive end

+

- A B DNA on murder weapon

3)Transgenic organisms

transgenic animal Mice--- so immune sys

more like humans- can study ‘em

Livestock --- leaner meat

carries a foreign gene, deliberately inserted into its genome.

transgenic plant Soybeans/corn/crops --- to

produce nat insecticiside so don’t have to be sprayed

Also: Improved Nutritional

Quality Insect Resistance Disease Resistance Drought Tolerance

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4) Cloning

replacing the nucleus of an unfertilized ovum with the nucleus of a body cell from the organism.

first adult mammal cloned was Dolly in 1997

Clone --- genetically identical copy

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5) DNA Fingerprinting Compare genetic similarities/differences b/w individuals. often used to ID source of blood / tissue found at crime

scenes. Polymerase Chain Reaction (PCR)

6) Gene Therapy the insertion of normal or genetically altered genes into cells usually to replace defective genes (cancer & genetic disorders)

Eye cancer

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My lab experience…….

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