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052615-1 Cell line: SW480 1 2 3 loading conc: 125 ug 24h, C 052615-2 Cell line: SW620 12 11 10 loading conc: 125 ug 24h, C Sample: 100uM drug treatment Tue code: !1

Gels

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calculations for WB gel

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052615052615-1Cell line: SW480123456789101112 (duplicate of 11)loading conc: 125 ug24h, C24h, veratMW48h, C48h, verat

052615-2Cell line: SW620121110987654321loading conc: 125 ug24h, C24h, veratMW48h, C48h, veratSample: 100uM drug treatmentTube code: R1

060515060515-1Cell line: SW480123456789101112loading conc: 150 ug24h, C24h, veratMW48h, C48h, verat

060515-2Cell line: SW620121110987654321loading conc: 150 ug24h, C24h, veratMW48h, C48h, veratSample: 100uM drug treatment in 10% DME; tx time= 10' and 30' Tube code: 10' or 30' label

060915060915-1Cell line: SW480123456789101112 (duplicate of 11)loading conc: 150 ug24h, C24h, veratMW48h, C48h, verat

060915-2Cell line: SW620121110987654321loading conc: 150 ug24h, C24h, veratMW48h, C48h, veratSample: Conservative readjustment based on mTOR expression of R1 samples (gels=052615)Tube code: R1

061815061815-1Cell line: SW480123456789101112loading conc: 150 ug24h, C24h, verat48h, C48h, verat

061815-2Cell line: SW620121110987654321loading conc: 150 ug24h, C24h, verat48h, C48h, veratSample: 100uM drug treatmentTube code: R2

062415062415-1Cell line: SW480123456789101112loading conc: 140 ug24h, C24h, verat48h, C48h, verat

062415-2Cell line: SW620121110987654321loading conc: 140 ug24h, C24h, verat48h, C48h, veratSample: 100 uM treatmentTube code: R3

070815070815-1Cell line: SW480123456789101112loading conc: adjusted based on mTOR expression on gel ()24h, C24h, veratMW48h, C48h, veratblank

070815-2Cell line: SW620121110987654321loading conc: adjusted based on mTOR expression on gel ()24h, C24h, veratMW48h, C48h, veratSample: Readjusted conc. Based on mTOR expression from R1 sample set (R1 gels = a) Tube code: R1

071015071015-1Cell line: SW480123456789101112loading conc: 150 ug24h, CMW24h, verat48h, C48h, verat

071015-2Cell line: SW620121110987654321loading conc: 150 ug24h, CMW24h, verat48h, C48h, veratSample: Re-reun of gels 061815Tube code: R2

071315

071315-2Cell line: SW620121110987654321loading conc: 150 ug24h, CMW24h, verat48h, C48h, veratSample: Re-run R3 samples (gel 062415-2 did not properly transfer) Tube code: R3

071515071515-1Cell line: SW480123456789101112loading conc: 150 ug24h, C24h, veratMW48h, C48h, veratblank071515-2Cell line: SW620121110987654321loading conc: 150 ug24h, C24h, veratMW48h, C48h, veratSample: 10uM drug treatmentTube code: A

071615071615-1Cell line: SW480123456789101112loading conc: 150 ug24h, C24h, veratMW48h, C48h, veratblank

Sample: Re-reun of gels 071515-1Tube code: A

071615b

071615-2Cell line: SW620121110987654321loading conc: 150 ug24h, C24h, veratMW48h, C48h, veratSample: Re-reun of gels 071015-2Tube code: R2

BB= 48h only samples tx w/100uM but in 60mm plates