IsolatMorfologi Koloni Morfologi Sel

Bentuk Elevasi Margin Bentuk Jenis Gram EndosporaU24 Punctiform Convex Entire Basil/Batang Gram positif -U36 Punctiform Flat Entire Basil/Batang Gram positif -U40 Punctiform Convex Curled Basil/Batang Gram positif -U45 Punctiform Flat Undulate Basil/Batang Gram positif -U47 Punctiform Flat Lobate Basil/Batang Gram positif -U57 Punctiform Flat Lobate Basil/Batang Gram positif -U58 Punctiform Flat Undulate Basil/Batang Gram positif -U59 Punctiform Flat Entire Basil/Batang Gram positif -U67 Punctiform Flat Undulate Basil/Batang Gram positif -U71 Punctiform Flat Curled Basil/Batang Gram positif -

Bacillus katalase (+), aerobik/ fakultatif anaerobik

1. B. megaterium: Large, smooth, soft, glistening, round, convex, entire, non-spreading, dense, creamy white to yellow. Variations: Rough, concentrically or radially ridged, thin edged.

2. B.cereus: Large, rough, flat, irregular with whip-like outgrowths. Whitish with characteristic mottled appearance by transmitted light (resembling galvanized iron or moire silk). Variations: Thin and spreading, rough and arboresce,nt, smooth and dense. Grayish, thin, widely spreading by means of long, twisted chains of cells, turning to the left or right (sinistral or dextral). The sinistral form occurs more often in soil

U24U36U47U57

U58U59U67U71U40U45

3. B. anthracis: Large, dense, irregular, composed of parallel chains of cells giving a curled or combed appearance; similar to certain strains of Bacillus cereus.

4. B. thuringiensis: sama kayak Bacillus cereus5. B. licheniformis: Large, spreading; surface rough or rugose; hairy outgrowths.

Offwhite. Variations: Smooth, entire or lobate 6. B. subtilis: Rough, opaque, dull, spreading, offwhite. Variations: Smooth to slimy,

soft, thin, translucent, dendroid. Yellow, orange or brown.7. B. pumilus: Smooth, thin, flat, spreading, dendroid, translucent. Variations: Small to

pinpoint, nonspreading, dense.8. B. coagulans: Usually small, round, opaque, not distinctive.9. B. badius: Large, dense, rough, opaque, with hairy outgrowths, resemble those of

Bacillus cereus.10. B. firmus: Small, round, smooth, dense, whitish, rarely pink.11. B. lentus: Small, round, smooth, dense, whitish, not distinctive.12. B. polymyxa: Usually thin, translucent, spreading, lobate or fimbriate. Rough stage

small, round, whitish, sometimes adherent13. B. macerans: Thin, transparent to whitish, spreading, irregular. Rough stage small

and compact.14. B. alvei: Thin, smooth, translucent, quickly spreading as a thin layer over entire

plate. Variation: Round and rather gummy. Giant colonies: Motile, bullet-shaped, micro-colonies moving in large arcs from point of inoculation, usually covering the plate in 1 day (agar plates should stand a couple days in order to dry the surface somewhat before using).

15. B. stearothermophilus: Not distinctive, pinpoint to small, round to irregular, translucent to opaque, rough to smooth.

16. B. circulans: Thin, spreading, translucent to transparent, sometimes barely visible or small to medium in size, opaque, entire. Giant colonies: Sometimes there are motile micro-colonies which move from the point of inoculation in a rotating pattern, eventually covering the entire plate (poured agar plates should be allowed to stand 2 or 3 days at room temperature in order to dry the surface somewhat before using).

17. B. laterosporus: Thin, translucent, spreading, irregular. Variations: Small, round, rough, opaque.

18. B. brevis: Thin, smooth, translucent, quickly spreading over entire plate. Variations: Small, round, opaque, non-spreading

19. B. panthothenticus: Small, round, translucent to dense, granular to moire, smooth to rough. Embedded colonies lenticular or irregular and hairy.

20. B. sphaericus: Thin, smooth, translucent, rapidly spreading over entire plate. Variations: Small, round or irregular. Giant agar colonies: Usually motile micro-colonies of various shapes move in large arcs from the point of inoculation and

cover the plate in 1 day (the surface of poured agar plates should be dried by holding at room temperature for 2 or 3 days before use)

Biokimia

IsolatIndole Acetic Acid

(IAA)Enzim Ekstraselular

KatalaseProtease Amilase Fitase Selulase

U24 - + - - - +U36 - + - - - +U40 - + + + + +U45 - + - + - +U47 - + + + - +U57 - + + + + -U58 - + + + - -U59 - + - - - +U67 - + + + + +U71 - + + - - +

Kemampuan degradasi pewarna

IsolatWarna

Merah Biru Hijau UnguU24 - - - -U36 - - - +U40 - - - +U45 - - - +U47 - - - +U57 - - - +U58 - - - +U59 - - - +U67 - - - +U71 + + - +

Isolat Media CairU24 -U36 -U40 -U45 -U47 -U57 -U58 -U59 -U67 -U71 -