GALAK Chromatography Technology

Reversion Phase Chromatography (RPC)

Galaksil® C18/C8 Prepacked Columns & Resins

GALAK Chromatography Technology

Galaksil® C18/C8

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Galaksil® C18/C8 Galaksil® is packing media of Reversion Phase

Chromatography (RPC) which uses for peptides

isolation and purification.

Galaksil® is a long carbon chain C18/C8 bound

with the spherical silica-gel substrate which has

low metal-ion content (<20 ppm), high specific surface area and good mechanical

strength. With GALAK® unique chemical bonding technique, Galaksil® has excellent

stability of bonding and end-capping. Hence, Galaksil® is preferred media for

industrial isolation.

Advantages

• Uniform particle size, High column efficiency, High resolution

• Low poriness, no fragments in repeated packing processes

• Excellent C18/C8 bonding and end-capping technologies

• Alkali resistance, easy for CIP operation

Multiple Choice

Galaksil® C18/C8 for common usage.

Galaksil® C18H/C8X have better alkali resistance.

GALAK Chromatography Technology 2

Reversion Phase Chromatography Column and Media

Main Characteristics

Base Material

Functional Size

Particle Size

Pore Size

Specific Surface Area

Carbon Content

End Caped

pH Range*

Bulk Density

Cleaning Agents

Silica gel

C18/C8

5/8/10/20 µm

120 Å

330m2/g

9-20%

Yes

pH 2-8 (2-11 for C18H/C8X)

Approx. 0.57 g/cm2

0.1-0.5M NaOH

Temperature Stability 4-25 °C

* pH stability, long term refers to the pH interval where the media is stable over a long period without

adverse effects on its subsequent chromatographic performance.

Product Information

Products Bonded Phase Bore Diameter Particle Size Carbon Content

C18 C18 120 Å 5/10/20 μm 15 %

C18H C18 120 Å 5/10/20 μm 20 %

C8 C8 120 Å 5/10/20 μm 12 %

C8X C8 120 Å 5/10/20 μm 9 %

GALAK Chromatography Technology

Galaksil® C18/C8

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Stability Test

20 repeated packing cycles in DAC column ran for Galaksil® C18 stability test.

Packing Condition

Equipment: DAC 150 system

Homogenization Buffer: 100% Isopropanol

Packing Material: Galaksil® C18H

Packing Pressure: 2000psi

Column Height: 250mm

Test Condition

Mobile Phase: 100% Methyl alcohol

Flow Rate: 350ml/min

Standard Substance: 0.5% Methylbenzene (v/v)

Injection Volume: 5ml

Determine Wavelength: 254nm

Results:

1st 5th 10th 15th 20th

250mm Theoretical Plates

Number（TP） 10191 10400 10294 10266 9398

Tailing Factor (TF) 1.31 1.33 1.29 1.05 1.35

Column Pressure / MPa 1.3 1.3 1.3 1.1 1.1

Note:

1. The performance of Galaksil® C18 changes little during repacking processes which conform to DAC

column requirements.

2. 100% isopropanol is for normal packing condition. For higher conditions, chloroform need to be

added.

3. The Permeability of sieve plate is very important for the performance of the column. The sieve plate

must be cleaned when the pressure rises.

GALAK Chromatography Technology 4

Reversion Phase Chromatography Column and Media

Details:

Chromatogram Electron Microscope

1st

5th

10th

15th

20th

8.4

92

'8

.55

8'

8.8

49

'

9.4

35

'

9.9

94

'

-10

0

10

20

30

40

50

60

70

80

90

100

mV

2 4 6 8 10 12 14 16 min

8.5

33

'

8.8

63

'

9.5

27

'9

.57

7'

10

.02

9'

12

.77

8'

-10

0

10

20

30

40

50

60

70

80

90

100

mV

2 4 6 8 10 12 14 16 min

2.7

73

'

4.3

98

'

6.3

24

'

8.5

18

'

8.8

34

'

9.9

92

'

-10

0

10

20

30

40

50

60

70

80

90

100

mV

2 4 6 8 10 12 14 16 min

8.4

12

'

9.7

87

'

10

.53

8'

11

.07

3'

-10

0

10

20

30

40

50

60

70

80

90

100

110

120mV

2 4 6 8 10 12 14 16 18 min

0.0

97

'

9.9

04

'

0

10

20

30

40

50

60

70

80

90

100

110

120mV

1 2 3 4 5 6 7 8 9 10 11 12 min

TP=10191

TF=1.31

Pre:1.3MPa

TP=10400

TF=1.33

Pre:1.3MPa

TP=10294

TF=1.29

Pre:1.3MPa

TP=10266

TF=1.05

Pre:1.1MPa

TP=9398

TF=1.35

Pre:1.1MPa

GALAK Chromatography Technology

Galaksil® C18/C8

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400V CIP clearance test for Galaksil® C8

Galaksil® C8 Competitive Product

Purification result for peptides after 400CV CIP process

Galaksil® C8 Competitive Product

GALAK Chromatography Technology 6

Reversion Phase Chromatography Column and Media

Standard Test

Standard Sample: Peptides (Sigma)

Galaksil® UP-C18 Competitive Product

Peak NO. Retention time Tailing factor

1 5.45 1.157

2 9.66 1.117

3 12.43 1.120

4 13.12 1.134

5 13.81 1.119

Peak NO. Retention time Tailing factor

1 5.27 1.113

2 9.50 1.097

3 12.27 1.097

4 12.98 1.124

5 13.67 1.093

Peptides Purification Application

Using Galaksil® UP-C18 and Competitive product in peptides purification. The

results show the performance of Galaksil® UP-C18 is good as competitive product.

The recovery purity of the final product all meet customer’s requirements.

Items Galaksil® UP-C18 Competitive Product

Performance Column Height(cm) 21.3 21.1

Column Efficiency (TP) 70457 56935

Peptides Injection Sample (g) 2.5 2.5

Recovery (%) 89.3 90.0

Purity (%) 95.5 95.5

Freeze-dried product (g) 1.1302 1.1317

GALAK Chromatography Technology

Galaksil® C18/C8

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Details:

Galaksil® UP-C18 Competitive Product

Notes:

1. The operation condition influences the result. Different personnel and equipment may have different

values and chromatograms.

2. The choice of packing material is according to the performance of the peptides.

3. The competitive product used in the tests are one of the bestsellers in the market.

Volume: 500ml

Injection Sample: 5μl

Peak Area: 10167

Purity: 67.2%

Volume: 500ml

Injection Sample: 5μl

Peak Area: 10213

Purity: 69.3%

Fr 42.7-52.9

Volume: 2040ml

Injection Sample: 20μl

Peak Area: 8902

Purity: 95.5%

Fr 42.1-54.1

Volume: 2400ml

Injection Sample: 20μl

Peak Area: 7665

Purity: 95.5%

GALAK Chromatography Technology 8

Reversion Phase Chromatography Column and Media

GALAK Chromatography Technology

Galaksil® C18/C8

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GALAK Chromatography Technology

88th Meiliang Rd, Mashan, Wuxi 214092, China

Tel: +86 510 85992929

Contact: jing.tian@galak-tech.com

Website: www.galak-tech.com (CN)

www.galaklc.com (EN)

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