Dr. Anu Prasanna Vankara, M.Sc., Ph.D. E-mail: annuprasanna@gmail.comAssistant Professor vap_2011@yahoo.comPrincipal Investigator CSIR No. 37(1592)/13/EMR-II

YVU/DEVP/VAP-CSIR/ Purchase of items/2013 Date: 06-11-2013

Quotations InvitedToThe Firms

Sub: Yogi Vemana University - Dept. of Zoology - CSIR project sanctioned to Dr. V. Anu prasanna - Purchase of Chemicals and glassware/plasticware from Ist year CSIR Contingent & Consumable grant- Call for quotations – Reg.

Ref: i) YVU/DEVP/VAP-CSIR/ Purchase of items/2013 dated 15.10.2013.

Sir/Madam, You are requested to send your lowest quotations in triplicate towards the supply of following chemicals and glasswares/ plasticwares along with your terms and conditions including clearance, custom duty, F O R to the Lab, so as to reach to “The Registrar, Yogi Vemana University, Vemanapuram, Kadapa-516003” on or before 30.11.2013 and kindly mention on envelop “Quotations for Dr. V. Anu prasanna-CSIR project- YVU/DEVP/VAP-CSIR/ Purchase of items/2013 ”.

Thanking you

Yours Sincerely

(V.Anu prasanna))

Department of ZoologyYOGI VEMANA UNIVERSITY KADAPA

Vemanapuram, Andhra Pradesh, India – 516 003

Chemicals (Himedia/Merck/Qualigens/Aldrich/Thermo Scientific):

Sl.No. Catalogue No. Company Chemical Name Quantity No. 1. ML014-100ML Himedia 0.5M EDTA, Ph 8.0 100ml 12. ML060-100ML Himedia 1X TE buffer, PH 8.0 100ml 1

3. ML016-100MLHimedia 50X TAE(Tris-acetate-EDTA)

buffer100ml

1

4. ML015-6X1MLHimedia 6X Gel loading Buffer (store at 2-

8˚C)6X1ml

15. AS024-500ML Himedia Acetone 500ml 26. ML036-100ML Himedia Acrylamide Solution (30%) 100ml 17. MB080-10G Himedia Agarose (Electrophoretic Grade) 10gm 18. GRM092-500G Himedia Aluminium ammonium sulphate 500gm 19. GRM167-500G Himedia Aluminium potassium sulphate 500gm 110. - Himedia Ammonium hydroxide MP 111. MB003-25G Himedia Ammonium persulphate, A.R 25gm 112. - Himedia Ammonium picrate MP 113. - Himedia Araldite/EPON RESIN MP 114. MB005-25GM Himedia Bis-Acrylamide (0.8%) 25gm 115. GRM325-500GM Himedia Boric acid crystals 500gm 116. 1004-125ML Himedia Bromophenol blue 125ml 217. - Merck Cacodylate/ Phosphate buffer MP 118. - Himedia Calcium chloride MP 119. GRM321-500GM Himedia Canada Balsam/ DPX mount 500gm 120. - Merck Carmine powder 5gm 221. AS039-500ML Himedia Chloroform 500ml 222. MB115-100ML Himedia Chloroform: Isoamyl alcohol 100ml 123. MB092-5G Himedia Coomassie brilliant blue 5gm 124. MB058-100ML Himedia DMSO (Dimethyl Sulphoxide) 100ml 1

25. MBT049-50LNHimedia DNA ladder 100bp/ DNA ladder

1kb 250µl 126. - Himedia DNA standard MP 127. MBT078-1ML Himedia dNTP solution 1ml 128. - Himedia Dodecyl Succinic anhydride MP 129. GRM678-100G Himedia EDTA 100gm 130. GRM6421-100G Himedia Egg Albumen powder 100gm 131. S007-125ML Himedia Eosin 125ml 132. MB071-1G Himedia Ethidium bromide 1gm 133. AS017-500ML Himedia Formaldehyde Sol, 37-41% 500ml 234. - Himedia Gelatin MP 135. GRM128-25G Himedia Giemsa powder 25gm 136. AS119-500ML Himedia Glacial Acetic acid. A.R 500ml 237. RM5927-25ML Himedia Glutarldehyde 25% 25ml 138. - Himedia Glycerine MP 139. AS100-500ML Himedia Glycerol (85%) 500ml 240. - Merck GOMORI’s Trichome MP 141. GRM236-5G Himedia Haemotoxylin crystals 5gm 1

42. S034-125ML Himedia Harris Haematoxylin solution 125ml 143. AS004-500ML Himedia Hydrochloric acid 500ml 244. GRM1064-50G Himedia Iodine crystals 50gm 145. AS068-500ML Himedia Isopropanol 500ml 146. GRM243-500ML Himedia Lactic acid 500ml 147. GRM949-25G Himedia Leishman stain powder 25gm 148. ML115-100ML Himedia Magnesium chloride Solution 100ml 149. - Merck Mallory solution MP 1

50. - Merck Mayer’s carmalum MP 1

51. - Merck Mercaptoethanol MP 1

52. - Merck Mercuric oxide MP 1

53. AS059-500ML Himedia Methanol 500ml 254. - - Methyl nadic anhydride MP 1

55. - - MilliQ water MP 1

56. 1008-125ml Himedia Neutral red indicator 125ml 157. - - Osmium tetraoxide MP 158. GRM1137-500GM Himedia Paraffin Wax 500gm 259. - Himedia PCR buffers MP 160. 1010-125ML Himedia Phenol red 0.02% indicator 125ml 261. GRM1788-500GM Himedia Picric acid extra pure 500Ggm 162. GRM209-500G Himedia Potassium Alum 500gm 263. GRM698-500G Himedia Potassium chloride 500gm 164. - Himedia Potassium hydrogen phosphate MP 165. GRM252-100G Himedia Potassium Iodide 100gm 1

66. -Himedia Primers (100bp- Biogene/Genetix

or 1kb Promega/Biogene) MP 167. MB086-10MG Himedia Proteinase-K 10mg 268. - Himedia Rectified spirit MP

69. ML007-100ML Himedia SDS (20%) 100ml 170. ML009-100ML Himedia Sodium acetate (pH 5.2) 100ml 171. - Himedia Sodium bicarbonate MP 172. ML008-100ML Himedia Sodium chloride 100ml 173. MB023-500G Himedia Sodium chloride 500gm 174. GRM6382-500G Himedia Sodium dihydrogen phosphate 500gm 175. ML026-100ML Himedia Sodium hydroxide 100ml 176. MB095-500G Himedia Sodium hydroxide 500gm 177. GRM7517-500G Himedia Sodium salicylate 500gm 178. - Himedia STE MP 179. - Himedia Sterile dH2O MP 180. - Himedia Sterilised Nanopure water MP 181. MB025-500G Himedia Sucrose pure 500gm 182. AS016-500ML Himedia Sulphuric acid (H2SO4) 500ml 2

83. MBT060D-500UHimedia Taq polymerase (1 unit/µl)

without MgCl₂500µ

184. MB026-100ML Himedia TEMED 100ml 185. - Himedia Template DNA

86. AS036-500ML Himedia Tetra Butyl alcohol (100%) 500ml 287. GRM7581-100G Himedia Thymol crystals 100gm 188. - Himedia TNE (Tris-NaCl-EDTA)- Buffer MP 189. MB030-100G Himedia Tris HCl 100gm 190. AS080-500ML Himedia Xylene,A.R 500ml 2

Note: Please quote the catalogue No. and the lowest available quantity for each chemical with amount in rupees. MP- Minimum pack (Quote)

Glassware (Borosil/ Merck)/ Plasticware (Tarsons):

Sl.No. (Make & Cat.No.) Glassware/ Plasticware Quantity/Number

1) Borosil-1519 Amber colour reagent bottle-250ml 102) Borosil-1519 Amber colour reagent bottle-500ml 103) Borosil-1000 Beakers -250ml 104) Borosil-1000 Beakers-500ml 105) Borosil Cavity blocks with lids 306) Borosil/Tarsons centrifuge tubes conical bottom (15ml) 1 case7) Borosil-5100 Conical flasks-250 ml 108) Himedia-PW354-1X100NO Coplin jars (Plastic) 209) Himedia-CG119-10X100NO Cover slips (18mm) 10 sets10) Himedia-CG120-10X100NO Cover slips (22mm) 10 sets11) Himedia-LA288-1x500NO Disposable Micropipette tips, (1µl-20µl) 1x50012) Himedia-LA286-1x500NO Disposable Micropipette tips, (200µl) 1x50013) Borosil/Tarsons Dissection instruments 3 Sets 14) Borosil/Tarsons Droppers (Glass) 2 Boxes15) Borosil Fine Brushes 2016) Riviera-71700 54/ Borosil Finger Bowls (150 x 80 ml) big size 1517) Riviera-71700 44/Borosil Finger bowls Small size 1518) Himedia Forceps 419) Borosil-6140-75mm Funnels 2020) Borosil/ Riviera Glass rods 1021) - Ice Storage Box (Big) 222) Borosil-3021 Measuring cylinder (1000ml) 123) Borosil-3021 Measuring cylinder (100ml) 224) Borosil-3022 Measuring cylinder (10ml) 225) Borosil-3021 Measuring cylinder (50ml) 226) Borosil-7002 Micropipette (variable 20-200 µl)) 127) Borosil-7002 Micropipette (Variable 2-20 µl) 128) Borosil-7002 Micropipette stand for three single channel

pipettes1

29) Himedia/Borosil Mortar and Pestle 330) - Ocular micrometer (10mm) 02

31) Himedia-LA017-1NO Para film M250 132) Himedia-CG281-1X1000NO PCR tubes with lids 2 sets

33) Borosil/Riviera Petri dishes (3” and 8”) 20 each34) Eutech-ECOPHTEST2-8020 009 pH tester 0135) Borosil/Tarsons Plastic vials -5ml & 10ml 100

each36) Himedia-GW192-1NO Prismatic Haemometer Square type 1

37) Borosil-1501/Tarsons Reagent bottles-250ml 1038) Borosil-1501 Reagent bottles-500ml 1039) - Silver foils 240) Riviera Slide boxes 1041) Bluestar/Riviera Slides (1.0-1.2mm thickness) 10

boxes42) - Spirit lamps 243) - Stage micrometer (1mm) 0144) - Table Lamp 245) Borosil Test tube cleaning brushes 2046) Borosil Test tube stands 547) Borosil/Tarsons Test tube stands 2sets48) Borosil Test tubes- 20ml 5049) Riviera-41 4100 Thermometer 250) Himedia Thumb press Dispensing Droppers 1x5051) Himedia-GW175-1NO. Tissue Homogenizer (Glass Vessel type S.P

10ml) 2 no.

52) Himedia-CG312-1x10NO/ Borosil Transfer Pipettes (0.5ML) 1053) Himedia-CG313-1x10NO/Borosil Transfer Pipettes (1 ML) 1054) Himedia-CG315-1x10NO/Borosil Transfer Pipettes (5ML) 1055) Himedia-LA611-1No Varivol II Micropipette-10 (variable 0.5-10

µl))1

56) Borosil-5641 Volumetric flasks (100ml) 1057) Borosil-5641 Volumetric flasks (250ml) 1058) Himedia-PW177-10NO Wash bottles 1059) Borosil-9985-125mm Watch glasses 20

60) Himedia Whatmann Filter paper 3 boxes