www.fungitell.com Add 100 µL of Fungitell ® lysate reagent into Pyroplate wells. 7 Read report data. 8 Fungitell ® Assay Report Date: Patient Beta-Glucan Interpretation I.D. pg/mL ~ ~ ~ ~ ~ 35 - ~ ~ ~ ~ ~ 70 n/a ~ ~ ~ ~ ~ 290 + 9 Incubate and collect kinetic data at 37º C for 40 minutes. 4 Reconstitute Fungitell ® Reagent. 5 100µL Add 20 µL of alkaline pretreatment solution into Pyroplate sample wells. 3 20µL Add 25 µL standards to Pyroplate. 6 25µL Incubate at 37º C for 10 minutes. 5 NOTE: For complete test procedure refer to Fungitell ® Instructions For Use (IFU). FUNGITELL ® ASSAY TEST PROCEDURE OUTLINE SET-UP TEST SAMPLES Reconstitute glucan stock and make standards. 1 Pipette 5 µL of sample into Pyroplate wells. 5µL 2

FUNGITELL ASSAY TEST PROCEDURE OUTLINE · TEST SAMPLES Incubate and collect kinetic data at 37º C for 40 minutes. 4 Reconstitute Fungitell® Reagent. 5 100µL Pipette 5 µL of sample

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Page 1: FUNGITELL ASSAY TEST PROCEDURE OUTLINE · TEST SAMPLES Incubate and collect kinetic data at 37º C for 40 minutes. 4 Reconstitute Fungitell® Reagent. 5 100µL Pipette 5 µL of sample

www.fungitell.com

Add 100 µL of Fungitell® lysate reagent into Pyroplate wells.

7 Read report data.8 Fungitell® Assay Report Date:

Patient Beta-Glucan Interpretation

I.D.

pg/mL

~ ~ ~ ~ ~ 35

- ~ ~ ~ ~ ~

70 n/a

~ ~ ~ ~ ~ 290

Reconstitute glucan stockand make standards.

1Prepare alkaline pre-treatment solution.

SET-UP

TEST SAMPLES

Incubate and collect kinetic data at 37º C for 40 minutes.

4Reconstitute Fungitell® Reagent.

100µL

Pipette 5 µL of sample into Pyroplate wells.

5µL

Add 20 µL of alkaline pretreatment solution into Pyroplate sample wells.

20µL

Add 25 µL standards to Pyroplate.

25µL

Incubate at 37º C for 10 minutes.

NOTE: For complete test procedure refer to Fungitell® Instructions For Use (IFU).

FUNGITELL® ASSAY TEST PROCEDURE OUTLINE

SET-UP

TEST SAMPLES

Add 100 µL of Fungitell® lysate reagent into Pyroplate wells.

7 Read report data.8 Fungitell® Assay Report Date:

Patient Beta-Glucan Interpretation

I.D.

pg/mL

~ ~ ~ ~ ~ 35

- ~ ~ ~ ~ ~

70 n/a

~ ~ ~ ~ ~ 290

Reconstitute glucan stockand make standards.

1Prepare alkaline pre-treatment solution.

SET-UP

TEST SAMPLES

Incubate and collect kinetic data at 37º C for 40 minutes.

4Reconstitute Fungitell® Reagent.

100µL

Pipette 5 µL of sample into Pyroplate wells.

5µL

Add 20 µL of alkaline pretreatment solution into Pyroplate sample wells.

20µL

Add 25 µL standards to Pyroplate.

25µL

Incubate at 37º C for 10 minutes.

Page 2: FUNGITELL ASSAY TEST PROCEDURE OUTLINE · TEST SAMPLES Incubate and collect kinetic data at 37º C for 40 minutes. 4 Reconstitute Fungitell® Reagent. 5 100µL Pipette 5 µL of sample

Corporate HeadquartersAssociates of Cape Cod, Inc.124 Bernard E. Saint Jean DriveEast Falmouth, MA 02536-4445 USATel: (888) 395–ACC1(2221) or(508) 540–3444Fax: (508) 540–8680www.acciusa.comCustomer Service:[email protected] Service:[email protected] Test Service:[email protected]

United KingdomAssociates of Cape Cod Int’l., Inc.Deacon Park, Moorgate RoadKnowsley, Liverpool L33 7RXUnited KingdomTel: (44) 151–547–7444Fax: (44) 151–547–7400E-mail: [email protected] Registration Number:BR002906

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