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Figures Only, Tables will be in the manuscript word file. Fungal Genome Size and Intron Evolution. Kemin Zhou, Oct 4, 2012. 400. 100. Batde5. ustma1. 350. 300. 80. 250. 200. 60. Sporo1. 150. 100. 40. 50. 0. 20. 40. 60. 80. 100. 0. 20. 40. 60. 80. 100. 0. 20. 40. 60. - PowerPoint PPT Presentation
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Fungal Genome Size and Intron Evolution
Kemin Zhou, Oct 4, 2012
Figures Only, Tables will be in the manuscript word file
Percent Relative Intron Location from 5’-End of mRNA
Co
un
t
10
02
00
30
04
00
Aspni1
10
01
50
20
02
50
Mycfi1
10
01
50
20
02
50
30
0
Mycgr1
20
03
00
40
05
00 Necha2
10
30
50
70
Picst3
10
02
00
30
04
00
Trive1
50
15
02
50
35
0
Trire2
30
05
00
70
0 copci1
10
02
00
30
04
00
cryneo1
40
06
00
80
01
000 Lacbi1
20
03
00
40
05
00 Phchr1
20
03
00
40
05
00
Pospl1
0 20 40 60 80 100
50
15
02
50
35
0
Sporo1
0 20 40 60 80 100
40
60
80
10
0
ustma1
0 20 40 60 80 100
10
02
00
30
04
00
Batde5
0 20 40 60 80 100
20
04
00
60
08
00
Phybl1
Fig 1.
Relative Intron Location0.0 0.2 0.4 0.6 0.8 1.0
0600
Avg = 0.501
Exon Length0 200 400 600 800 1000
01500
Avg = 214.8
Number of Introns per Gene0 10 20 30 40
0600
Avg = 7.6 round = 0
Relative Intron Location0.0 0.2 0.4 0.6 0.8 1.0
0600 Avg = 0.476
Exon Length0 200 400 600 800 1000
02000 Avg = 246.2
Number of Introns per Gene0 10 20 30 40
0600 Avg = 6.5 round = 1
Relative Intron Location0.0 0.2 0.4 0.6 0.8 1.0
0400
Avg = 0.456
Exon Length0 200 400 600 800 1000
02000 Avg = 276.9
Number of Introns per Gene0 10 20 30 40
0600 Avg = 5.7 round = 2
Relative Intron Location0.0 0.2 0.4 0.6 0.8 1.0
0400
Avg = 0.427
Exon Length0 200 400 600 800 1000
01500 Avg = 332.3
Number of Introns per Gene0 5 10 15 20 25 30 35
01000 Avg = 4.6 round = 4
Relative Intron Location0.0 0.2 0.4 0.6 0.8 1.0
0400 Avg = 0.407
Exon Length0 200 400 600 800 1000
01000 Avg = 412.1
Number of Introns per Gene0 5 10 15 20 25 30 35
01500 Avg = 3.5 round = 8
Relative Intron Location0.0 0.2 0.4 0.6 0.8 1.0
0300
Avg = 0.419
Exon Length0 200 400 600 800 1000
0800 Avg = 493.3
Number of Introns per Gene0 5 10 15 20 25 30 35
03000
Avg = 2.7 round = 16
Relative Intron Location0.0 0.2 0.4 0.6 0.8 1.0
0300 Avg = 0.453
Exon Length0 200 400 600 800 1000
0600 Avg = 551.9
Number of Introns per Gene0 5 10 15 20 25 30
03000 Avg = 2.4 round = 32
0.0 0.2 0.4 0.6 0.8 1.0
0200 Avg = 0.481
0 200 400 600 800 1000
0600 Avg = 583.7
0 5 10 15 20 25 30
04000 Avg = 2.2 round = 64
Fig 2.
RIL Exon Length IPG
0.42 0.44 0.46 0.48 0.50
23
45
67
8
Relative Intron Location
IPG
0.42 0.44 0.46 0.48 0.50
4.5
5.5
6.5
7.5
Relative Intron Location
IPG
0 50000 150000 250000
23
45
67
Number of RT
2 4 6 8 10 12
23
45
67
ln(Number of RT)
-0.13
Fig 3.
A.
B.
C.
D.Number of RT Events
Ln (Number of RT Events)
789.210089.2948.4 610718.4 5
xey x
Mean Relative Intron Location
Me
an
Nu
mb
er
of
Co
din
g E
xon
s p
er
Ge
ne
0.40 0.42 0.44 0.46 0.48 0.50
12
34
56
78
Aspni1
Batde5
copci1
cryneo1
Lacbi1
Mycfi1Mycgr1
Necha2
Phchr1
Phybl1
Picst3
Pospl1
Sporo1
Trire2Trive1
ustma1
7.66y = 61.94x – 23.313p-value = 2.566E-07
Fig 4.
0 200 400 600 800 1000 1200 1400
23
45
67
8
Number of RT
EP
G
Fig 5.
Number of RTF
389.210089.2848.4 510118.2 3
xey x
Fig 6.
Intercept: 9.69 ± 1.99Slope: -0.30 ± 0.16
Intercept: 4.04 ± 0.35Slope: -0.11 ± 0.03
9 10 11 12 13 14
23
45
67
ln (Total RTF Length (nt))
Ave
rag
e E
PG
Aspni1
Batde5
copci1
cryneo1
Lacbi1
Mycfi1
Mycgr1Necha2
Phchr1
Phybl1
Picst3
Pospl1
Sporo1
Trire2
Trive1
ustma1
0 100 200 300 400
010
0020
0030
00
Cou
nt3n3n+13n+2
0 100 200 300 400
050
150
250
Cou
nt
3n3n+13n+2
Exon Length (nt)
C. reinhardtii
FungiA.
B.
Fig 7.
75
Number of Introns per Gene
Ave
rag
e E
xon
Le
ng
th (
nt) 5.1988961.11.1060 7812.0 xey x
020
060
010
00
0 10 20 30 40 50 60 70
Fig 8.
Percent relative intron location from 5’-end of mRNA
Diff
ere
nce
of
fre
qu
en
cy (
no
n-c
on
serv
ed
- c
on
serv
ed
)
-0.0
40.
000.
04 1.204E-07
Aspni1
1.482E-18
Mycfi1
2.228E-10
Mycgr1
1.390E-25
Necha2
-0.0
40.
000.
04 1.303E-03
Picst3
1.397E-35
Trive1
5.121E-18
Trire2
4.021E-08
copci1
-0.0
40.
000.
04 5.447E-01
cryneo1
1.770E-35
Lacbi1
1.330E-01
Phchr1
4.135E-02
Pospl1
0 20 40 60 80
-0.0
40.
000.
04 8.968E-02
Sporo1
0 20 40 60 80
2.997E-03
ustma1
0 20 40 60 80
8.028E-11
Batde5
0 20 40 60 80
1.047E-22
Phybl1
Fig 9.
Fig 10.
allbetween phylaphylumspecies
0
2
4
6
8
0
1
2
3
4
5
6
7
copc
i1
cryn
eo1
Lacb
i1
Phchr
1
Pospl1
Sporo
1
ustm
a1
Batde
5
Phybl1
Aspni1
Myc
fi1
Myc
gr1
Necha
2
Picst3
Trire
2
Trive
1
A.
B.
EP
G p
er
40
0 a
aE
PG
EPG of Genes Conserved in All Species
EP
G o
f S
pe
cie
s-sp
eci
fic G
en
es
2 3 4 5 6 7 8
23
45
67
Aspni1
Batde5
copci1
cryneo1
Lacbi1
Mycfi1
Mycgr1
Necha2
Phchr1
Phybl1
Picst3
Pospl1
Sporo1
Trire2
Trive1
ustma1
y = 0.503 x + 1.172
Excluding Sporo1, p-val=8.196E-07
Fig 11.
