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COST Action MP1301
FTM/Faculty of Technology and Metallurgy
FTMFaculty of Technology and MetallurgyUniversity of BelgradeKarnegijeva 411000 Belgrade - SERBIA
� Complete denomination: Faculty of Technology and Metallurgy, University of Belgrade
� Location (city, country): Belgrade, Serbia
� Dean: Prof. Dr Djordje Janackovic
� Contact person in NEWGEN: Prof. Dr Bojana Obradovic
� Working Group involvment: WG4
� Staff: Dr Jasmina Stojkovska, Jovana Zvicer, Mina Jovanovic,
Dr Djordje Veljovic, Andrea Osmokrovic
� Research topics: biomimetic bioreactors, skeletal tissue engineering,
nanocomposite hydrogels
� Researchers expertises: bioreactor design and operation,
biomaterial evaluation under biomimetic conditions
GENERAL PRESENTATION
COST Action MP1301
BIOMIMETIC BIOREACTORS
FTM/Faculty of Technology and Metallurgy
Biomimicking articular cartilage:perfusion: 10 – 100 µm/sdynamic compression: 337.5 µm/s, 0.1 - 1 Hz, 5-10 % strain
Bioreactor with dynamic compression Perfusion bioreactors
medium
Bioreactor with hydrostatic pressures
up to 30 bar; continuous perfusion
actuator
specimenoil
membranemicrometerscrew
loadsensor
diaphragm
sample
medium in
medium out
COST Action MP1301
Novel Ag/alginate nanocomposites
FTM/Faculty of Technology and Metallurgy
Production of Ag/alginate hydrogels starting from the Ag/alginate solution:
Production of novel Ag/alginate nanocomposites with incorporated silver nanoparticles (AgNPs)
Electrochemical synthesis of AgNPs in alginate solution:
alginatesolution
Ca(NO3)2
+
syringepump
needleAg/alginatemicrobeads
peristalticpump
needleCa(NO3)2
Ag/alginatesolution Ag/alginate
microfibers
Novel Ag/alginate nanocomposites
500 µm 500 µm
Ag/alginate microfibersinitial dried
500 µm
Ag/alginate microbeadsinitial dried
500 µm
Ag/alginate solutions with controlled AgNPs:concentration
low high
y = 3.1406x
R2 = 0.9989
0
0.2
0.4
0.6
0.8
1
1.2
1.4
0 0.1 0.2 0.3 0.4
c [mM]
abso
rban
ce a
t 40
5 n
m
TEM image
size
microbeads microfibers
Bioreactor studies:- biomechanical characterization- Ag release- cell-biomaterial interactions- performance under biomimetic conditions
Hydrothermal synthesis of AgNPs in alginate solutions
AgNP synthesis by chemical reduction in alginate/honey solutions
COST Action MP1301
CYTOTOXICITY STUDIES IN BIOREACTOR CULTURES
FTM/Faculty of Technology and Metallurgy
dynamic compression:1h on/ 1 h off, 0.56 Hz, 10 % straincontinuous perfusion:0.18 ml/min, 15 µm/s
cell/alginate microbead
isolate cells or
Ag/alginate microbeads
explants
strong cytotoxicity in 2D culturesno cyctotoxicity in 3D cultures consistent with in vivo results
3D tissue culture systems provide more reliable functional evaluation of nanomaterials
COST Action MP1301
CHARACTERIZATION OF GELLAN-GUM HYDROGELS REINFORCED WITH BIOACTIVE-GLASS
FTM/Faculty of Technology and Metallurgy
In collaboration with dr Sasa Novak, Jozef Stefan Institute, Slovenia
Hydroxyapatite formation in 2 % w/w gellan-gum discs with 2 % w/w of bioactive-glass (composition: 70 % SiO2, 30 % CaO) over 14 days of continuous perfusion of simulated body fluid
Zvicer J., Gantar A., Veljovic Dj., Novak S., Obradovic B., Evaluation of nano-particulate bioactive-glass reinforced gellan-gum hydrogel regarding the formation of hydroxyapatite under shear stress, Seventeenth Annual Conference YUCOMAT 2015, 31.08- 4.09, 2015, Herceg Novi, Montenegro, Programme & The Book of Abstracts, p. 87
initial
14 d
� Flow rate 1.13 ml/min� SBF exchange every 48 h� 37 oC
RAMAN apectroscopy: characteristic pick for HAp1 at ̴ 960 nm was obtained in all checked spots
FEG-SEM
sample cross-section