REFERENCES

﴿ ﴾

116

7. REFERENCES

Bartels, F. and Bigalke, H. (1992).

Restoration of exocytosis occurs after inactivation of intracellular

tetanus toxin.

Infect. Immun., 60( 1): 302-307.

Bizzini, B.; Turpin, A. and Raynaud, M. (1973).

On the structure of tetanus toxin .

Arch. Pharm., 276: 271-288.

Blood, D.C; Radostits, O.M. and Henderson, J.A. (1983).

Veterinary Medicine.

6th Ed. Pitman Press Ltd. Great Britain PP. 538-553.

Boushaba, R; Kumpalume, P. and Slater, N.K.(2003)

Kinetics of whole serum and prepurified IgG digestion by pepsin

for F (ab')2 manufacture.

Biotechnol Prog., 19(4): 1176-82.

Bradford, M.M. (1976).

A rapid and sensitive method for the quantitation of microgram

quantities of protein utilizing the principle of protein-dye binding.

Anal. Biochem., 72: 248-254.

Brewer, J. H. (1940).

Clear liquid medium for aerobic cultivation of anaerobes.

J. Amer. Med. Ass., 115: 598.

REFERENCES

﴿ ﴾

117

Burns, R.(2002).

Antibody production In: The Protein Protocols Handbook. 2nd

Edition, Humana Press, Totowa, New Jersey.

Burnouf, T. ;Terpstra, F.; Habib G. and Salwa S. (2007).

Assessment of viral inactivation during pH 3.3 pepsin digestion

and caprylic acid treatment of antivenoms.

Biologicals, 35: 329-334.

Butler, J.E; Josh, K.S and Brown, W.R.(1972)

Toxins and antitoxins. 3rd Ed. In: Experimental Immunology.

Issac Pitman and Sons Ltd. p. 69–80.

Caleo, M. and Schiavo, G. (2009).

Central effects of tetanus and botulinum neurotoxins.

Toxicon, 54: 593–599.

Carter, G.R. and Darla, J. W.(2004).

Essentials of Veterinary Bacteriology and Mycology. 6th Edition

Iowa State Press, Blackwell USA.

Cheung, G. L.M.; Theresa M. Thomas, and Dennis B. Rylatt (2003)

Purification of antibody Fab and F(ab)2 fragments using Gradiflow

technology

Protein Expr. Purif., 32: 135–140.

REFERENCES

﴿ ﴾

118

Chin, J.; Sohn, Y.; Lee, S.H.; Park, Y.I. and Choi, M.J. (2003).

Production of neutralizing human monoclonal antibody directed to

tetanus toxin in CHO cell.

Biologicals, 31(1): 45-53.

Clare, J.; Sreekrishna, K. and Romanos, M. (1998).

Expression of tetanus toxin fragment C

Methods Mol Biol., 103: 193-208.

Cresswell, C.; Newcombe, A.R.; Davies, S.; Macpherson, I.; Nelson,

P.; O'Donovan, K. and Francis, R. (2005).

Optimal conditions for the papain digestion of polyclonal ovine

IgG for the production of bio-therapeutic Fab fragments.

Biotechnol. Appl. Biochem., 42(Pt 2):163-8.

Cruickshank, R.D.; Dugid, J.P.; Mermion, B.R. and Swain, R.H.

(1975).

Medical Microbiology.

2nd vol., 12th Ed., Churchill Livingstone, Edinburgh, London, New

York.

Demain, A.L.; Gerson, D.F.; Kole, M. and Fang, A. (2006):

The role of reduced iron powder in the fermetative production of

tetanus toxin.

Appl. Microbiol. Biotechnol., 73 (1): 55-59.

Duffy, J.I. (1980)

Tetanus in: Vaccine Preparation Techniques. Noyes Data

Corpration. New Jersey, USA.

REFERENCES

﴿ ﴾

119

Favreau, B. ;Giurgiu, D. and Bizzini, B. (1983).

A new method for the large scale preparation of antitoxic

antibodies exhibiting high specific protective activities.

Expreientia, 39: 483-487.

Fernandes, A.; Kaundinya, J.O.; Daftary, G.; Saxena, L.; Banerjee,

S. and Pattnaik, P.(2008).

Chromatographic purification of equine immunoglobulin G F(ab)2

from plasma.

Chromatogr. B Analyt. Technol. Biomed. Life Sci., 876(1):109-15.

Finn, C. W.; Silver, R. P.; Habig, W. H.; Hardegree, M. C.; Zon, G.

and Garon, C. F.(1984).

The structural gene for tetanus toxin is on a plasmid.

Science, 224: 881-884.

Fisek, N. H.; Mueller, J. H. and Miller, P. A. (1954).

Muscle extractives in the production of tetanus toxin.

J. Bact., 67: 329-334.

Fischer, P.M. and Howden, M.E.(1994)

Synthetic peptide antigens of tetanus toxin.

Mol. Immunol., 31(15): 1141-8.

Fishman, P.S.; Farrand, D.A.; Halpern, J.L. and Latham,

W.C.(1992)

A simplified method for the preparation of tetanus toxin binding

fragment for neurobiology.

J. Neurosci. Methods., 42(3): 229-36.

REFERENCES

﴿ ﴾

120

FIu, G.; VIu, P.; Komarova, E.A. and Sheremet'ev, N.N. (2002).

Study on the effect of a biostimulant on the growth and toxigenic

function of Clostridium tetani strain Copenhagen-471.

Zh. Mikrobiol. Epidemiol. Immunobiol., (6): 73-75.

Foster, K. A. (2009)

Engineered toxins: New therapeutics.

Toxicon, 54: 587–592.

Francis, J.W.; Bastia, E.; Matthews, C.C.; Parks, D.A.;

Schwarzschild, M.A.; Brown, R.H.Jr. and Fishman, P.S. (2004).

Tetanus toxin fragment C as a vector to enhance delivery of

proteins to the CNS.

Brain Res., 1011(1): 7-13.

Fratelli, F.; Siquini,T.J.; Prado,S.M.; Higashi,H.G.;Converti, A.; de

Carvalho, J.C. (2005).

Effect of medium composition on the production of tetanus toxin

by C. tetani

Biotechnol. Prog., 21(3):756-61.

Fratelli, F.; Siquini, T.J.; de Abreu, M.E.; Higashi, H.G.; Converti,

A. and de Carvalho, J.C.(2010)

Fed-batch production of tetanus toxin by Clostridium tetani.

Biotechnol Prog., 26(1): 88-92.

REFERENCES

﴿ ﴾

121

Grodzki, A.C. and Berenstein, E.(2010)

Antibody purification: ion-exchange chromatography.

Methods Mol. Biol., 588: 27-32.

Goretzki, K. and Habermann, E.(1985)

Enzymatic hydrolysis of tetanus toxin by intrinsic and extrinsic

proteases. Characterization of the fragments by monoclonal

antibodies.

Med. Microbiol. Lmmunol., 174: 139—150.

Guidolin, R.; Morais, J.F.; Stephano, M.A.; Marcelino, J.R.;

Yamaguchi, I.K. and Higashi, H.G.(1997)

Effect of beta-propiolactone treatment on the complement

activation mediated by equine antisera.

Rev. Inst. Med. Trop. Sao. Paulo., 39(2):119-122.

Harlow, E. and Lane, D. (1988).

Antibodies.

A Laboratory Manual. Cold Spring Harbor Lab., N.Y.

Hatamabadi, H.R. ; Abdalvand, A.; Safari,S.; Kariman,H.;

Dolatabadi, A. A. ; Shahrami,A. Alimohammadi, H. and Hosseini,

M. (2010).

Tetanus Quick Stick as an applicable and cost-effective test in

assessment of immunity status.

Am. J. of Emerg. Med. , Apr 30. [Epub ahead of print]

REFERENCES

﴿ ﴾

122

He, H.J.; He, Z.Y.; Shi, H.J.; Zhu, W.; Yang, G.Z.; Yuan, Q.S. and

Wu, X..F(2000).

Cloning and Expression of Tetanus Toxin Fragment C in E.coli.

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao(Shanghai)

32(4): 322-326.

Helting, T. B and Zwisler, O. (1974).

Enzymatic breakdown of tetanus toxin.

Biochem. Biophys. Res. Comm., 57: 1263-1270.

Helting, T. B and Zwisler, O.(1977).

Structure of tetanus toxin. I. Breakingdown of the toxin molecule

and discrimination between polypeptide fragments.

J. Biol. Chem., 252: 187-193.

Helting, T. B.; Ronneberger, H.; Vollerthun, R. and Neubauer, V.

(1978).

Toxicity of papain-digested tetanus toxin. Pathological effect of

fragment B in the absence of spastic paralysis.

J. Biol. Chem., 253(1): 125-129.

Hepple, J. R.(1968).

Large-scale production of Clostridium tetani toxin

Chemistry and Industry, 21: 670-674.

REFERENCES

﴿ ﴾

123

Herrera, M.; Leon, G.; Segura, A.; Meneses, F.; Lomonte, B.;

Chippaux, J.P. and Gutierrez, J. M. (2005)

Factors associated with adverse reactions induced by caprylic acid-

fractionated whole IgG preparations: comparison between horse,

sheep and camel IgGs.

Toxicon, 46: 775-781.

Herreros, J.; Lalli, G. and Schiavo G. (2000)

C-terminal half of tetanus toxin fragment C is sufficient for

neuronal binding and interaction with a putative protein receptor.

Biochem J., 347 Pt 1:199-204

Hervé, F.; Ghinea, N.; D'Athis, P.; Carrupt, P.A. and Scherrmann,

J.M. (2008).

Covalent modifications of antitetanus F(ab')2 fragments with

natural and synthetic polyamines and their effects on the antibody

endocytosis in cultured HL60 cells.

Bioconjug, Chem., 19(8):1543-55.

Holliger, P. and Hudson, P.J. (2005)

Engineered antibody fragments and the rise of single domains.

Nature biotechnology, 23(9): 1126-1136.

Ikbal, F.; Nagi, G. M. and Ebeid, M. H.(1988).

Factors affecting production and stability of tetanus toxins.

Vet. Med. J., 36(2): 267-278.

REFERENCES

﴿ ﴾

124

Jhonson, E. A. (2005)

Clostridium botulinum and Clostridium tetani. In: Toply and

Wilson ُ s Microbiology and Microbial Infections, 10th Edition.

Hodder Arnold, London.

Jones, R.G.A and Landon, J.(2003).

A protocol for "enhanced pepsin digestion": a step by step method

for obtaining pure antibody fragment in high yield from serum.

J.Immunol. Methods., 275: 239-250.

Kegel, B.; Behrensdorf-Nicol, H.A.; Bonifas, U.; Silberbach, K.;

Klimek, J.; Kra¨mer, B. and Weißer, K. (2007).

An in vitro assay for detection of tetanus neurotoxin activity:Using

antibodies for recognizing the proteolytically generated cleavage

product.

Toxicology in Vitro, 21: 1641–1649.

Kegel, B.; Bonifas, U.; Silberbach, K.; Kramer, B. and Weisser, K.

(2002).

In vitro determination of specific toxicity in tetanus vaccines.

Dev. Biol. (Basel). 111: 27-33.

Krieglestein, K.; Henschen, A.; Weller, U. and Habermann, E.(1990)

Arrangement of disulfide bridges and positions of sulfhydryl

groups in tetanus toxin.

Eur. J. Biochem., 188: 39-45.

REFERENCES

﴿ ﴾

125

Kumpalume, P.; Boushaba, R. ; Jones R. G. A and Slater N. K.

H.(2002).

Facile Fab2 manufacturing: strategies for the production of snake

antivenoms.

Trans IChemE, 80( Part C): 88-97.

Laing, G. D. Lee L., Smith, D. C. Landon J. and Theakston R. D.

G.(1995)

Experimental assessment of a new, low-cost antivenom for

treatment of carpet viper (Echis ocellatus) envenoming.

Toxicon, 33(3): 307-313.

Latham, W. C.; Bent, D. F. and Levine, L.(1962).

Tetanus toxin production in the absence of protein.

App. Microbiol.,10: 146-152.

Latham, W.C.; Jenness, C.P.; Timperi, R.J.K.; Michelsen, C.B.H.;

Zipilivan, E.M.; Edsall, G. and Ley, H.J.Jr. (1965).

Purification and characterization of tetanus toxoid and toxin I.

Fractionation of tetanus toxoid by gel filtration.

J. Immunol., 95: 487-493.

Le H. Duc, Huynh A. Hong, Neil Fairweather, Ezio Ricca, and Simon

M. Cutting. (2003).

Bacterial Spores as Vaccine Vehicles

Infection and Immunity, 71(5): 2810–2818.

REFERENCES

﴿ ﴾

126

Ledoux, D.N.; Be, X.H. and Sin, B.R. (1994)

Quaternary structure of botulinum and tetanus neurotoxins as

probed by chemical cross-linking and native gel electrophoresis.

Toxicon., 32(9): 1095-104.

Lee, G. H. (2000).

High-performance sodium dodecyl sulfate-capillary gel

electrophoresis of antibodies and antibody fragments.

J. Immunol. Methods, 234 :71–81.

Leslie, H. and Frank, C. (1989).

Practical Immunology.

3rd Ed., Blackwell Scientific Publications.

Maloy, S. (1990):

Supplemental assay method for potency testing of tetanus toxoid

by ELISA.

United States Dep. Of Agric. Animal and Plant Health Inspection

Service, Iowa, USA.

Maria, M. D.; Abeiro, H. D.; Bernagozzi, J. A. and Juan, A. B.

(1997).

Nitrogen gas bubbling during the cultivation of Clostridium tetani

produces a higher yield of tetanus toxin for the preparation of its

toxoid.

Microbiol. Immunol., 41(2):161-163.

Matsuda, M. and Yoneda, M. (1974).

Dissociation of tetanus neurotoxin into two polypeptide fragments.

Biochem. Biophys. Res. Comm., 57(4): 1257-1262.

REFERENCES

﴿ ﴾

127

Matsuda, M. and Yoneda, M. (1975).

Isolation and purification of two antigenically active"

complementary" polypeptide fragments of tetanus neurotoxin.

Infect. Immun., 12(5): 1147-1153.

Mellanby, J. (1968).

The effect of glutamate on toxin production of Clostridium tetani.

J. Gen. Microbiol., 54: 77-82.

Mendieta, L.; Venegas, B.; Moreno, N.; Patricio, A.; Martínez,

I.;Aguilera J. and Limón, I.D.(2009)

The carboxyl-terminal domain of the heavy chain of tetanus toxin

prevents dopaminergic degeneration and improves motor behavior

in rats with striatal MPP(+)-lesions.

Neurosci Res., 65(1): 98-106.

Miana-Mena, F.J.; Munoz, M.J.; Ciriza, J.; Soria, J.; Brulet, P.;

Zaragoza, P.; and Osta, R. (2003)

Fragment C tetanus toxin: a putative activity-dependent

neuroanatomical tracer.

Acta. Neurobiol. Exp. (Wars)., 63(3): 211-8.

Michella M. and Parkinson, A. (1987).

A simple non-chromatographic procedure to purify

immunoglobulins from serum and ascitis fluid.

J. Immunol. Methods, 96: 271-278.

REFERENCES

﴿ ﴾

128

Mohanty, J.G. and Elazhary, Y.(1989)

Purification of IgG from serum with caprylic acid and ammonium

sulphate precipitation is not superior to ammonium sulphate

precipitation alone.

Comp. Immunol. Microbiol. Infect. Dis., 12(4): 153-60.

Morais,V. and Massaldi, H. (2005)

Effect of pepsin digestion on the antivenom activity of equine

immunoglobulins.

Toxicon, 46(8): 876-82.

Morais,V. and Massaldi, H. (2007)

Evolution of endotoxin contamination during production of a

therapeutic serum.

PDA J. Pharm. Sci. Technol., 61(5): 375-82.

Mpandi, M.; Schmutz, P.; Legrand, E.; Duc, R.; Geinoz, J.;

Henzelin-Nkubana, C.; Giorgia, S.; Clerc, O.; Genoud, D. and Webe,

T. (2007)

Partitioning and inactivation of viruses by the caprylic acid

precipitation followed by a terminal pasteurization in the

manufacturing process of horse immunoglobulins.

Biologicals, 35(4): 335-41.

Mueller, J. H. and Miller, P. A. (1954) .

Variable factors influencing the production of tetanus toxin.

J. Bact., 67: 271-277.

REFERENCES

﴿ ﴾

129

Nadia, M. E. (1992).

Studies on the toxins of Clostridium tetani.

M.V.Sc. Thesis, Microbiology (Bacteriology), Faculty of

Veterinary Medicine, Zagazig University.

Nakajima, Y.; Sakamoto, M.; Nishi, K.; Nishio, S.; Oosone, Y.;

Akizuki. S. and Nozaki, H. (2009)

Two severe tetanus cases infected in an urban area of Kawasaki

City.

Kansenshogaku Zasshi., 83(6): 679-82.

Nielsen, P.A. (1967).

Large-scale Production of tetanus toxoid

Appl. Microbiol., 15(2): 453-454.

Oconnor, L. (2006)

The analysis of bacterial protein by SDS-PAGE. Diagnostic

Bacteriological Protocols: Methods in molecular biology,46: 27-

40.

Odendaal, M. W. and Kriek, N. P. J. (1994)

Tetanus in: Infectious Diseases of Livestock. Oxford University

Press, London.

Anon (1998)

Oxoid Manual,8th Edition, published by Oxoid limited, Wade

Road, Basingstoke, Hampshire, England.

REFERENCES

﴿ ﴾

130

Ozutsumi, K.; Sugimoto, N. and Matsuda, M.(1985).

Rapid, simplified method for production and purification of tetanus

toxin.

Appl. Env. Micrbiol., 49(4): 939-943.

Ozutsumi, K.; Lei, D.L.; Sugimoto, N. and Matsuda, M. (1989).

Isolation and purification by high performance liquid

chromatography of a tetanus toxin fragment (fragment [A-B])

derived from mildly papain-treated toxin.

Toxicon, 27(9): 1055-1057.

Page, M and Thrope, R. (1998).

Immunochemical Protocols (Methods in Molecular Biology), 2nd

Ed. Humana Press, Totowa, New Jersey.

Poulain, B.; de Paiva, A.; Dolly, J.O.; Weller. U. and Tauc. L. (1992)

Differences in the temperature dependencies of uptake of

botulinum and tetanus toxins in Aplysia neurons.

Neurosci Lett., 139(2): 289-92.

Prado, S.M.; Vancetto, M.D.; de Oliveira, J.M.; Fratelli, F. and

Higashi, H. G.(1999)

Development and validation study for the chromatographic

purification process for tetanus anatoxin on Sephacryl S-200 High

Resolution.

Boll. Chim. Farm.,138(7):364-8.

REFERENCES

﴿ ﴾

131

Qazi, O; Sesardic, D; Tierney, R; So¨derba¨ck, Z; Crane, D;

Bolgiano, B and Fairweather, N. (2006)

Reduction of the ganglioside binding activity of the tetanus toxin

HC fragment destroys immunogenicity: implications for

development of novel tetanus vaccines.

Infect. Immun., 74(8): 4884–4891.

Rawat, S.; Laing G.;Smith, C.; Theakstone, R.G. and

Landon,J.(1994).

A new antivenom to treat eastern coral snake (Micrurus fulvius

fulvius).

Toxicon, 32(2): 185-190.

Raweerith R and Ratanabanangkoon K. (2003).

Fractionation of equine antivenom using caprylic acid precipitation

in combination with cationic ion-exchange chromatography.

J. Immunol. Methods., 282(1-2):63-72.

Rea, D. W. and Ultee, M.E. (1993).

A novel method for controlling the pepsin digestion of antibodies.

J Immunol Methods, 157(1-2): 165-173.

Redwan, E. M.; Khali A. and Zeinab EL-Dardiri (2005).

Production and purification of ovine anti-tetanus antibody.

Comp. Immunol. Microbiol. Infect. Dis., 28: 167–176.

REFERENCES

﴿ ﴾

132

Redwan, E. M. (2006).

Comparison between therapeutic antitoxin F(ab)2 fractionated with

ammonium sulphate and caprylic acid.

J. immunoassay immunochem., 27: 319-329.

Robinson, J.P.(1988).

Purification of tetanus toxin and its major peptides

Methods In Enzymology. 165: 85-90.

Rodrigues-Silva R. ; Martins M. S.; Magalhaes A. and Santoro M.

M.(1997)

Purification and stability studies of Immunoglobulins from lachesis

muta muta antivenom.

Toxicon, 35(8):1229-1238.

Rojas, G.; Jimenez, J. M. and Gutierrez, J. M. (1994).

Caprylic acid fractionation of hyperimmune horse plasma:

description of a simple procedure for antivenome production.

Toxicon, 32: 351-363.

Rossetto, O; Sevesoa, M; Caccina, P; Schiavo, G; and Montecucc, C.

(2001)

Tetanus and botulinum neurotoxins: turning bad guys into good by

research.

Toxicon, 39: 27-41.

REFERENCES

﴿ ﴾

133

Salwa, S. A.; Wanas, S.; Shehata, A.; Fawaz, S. and Helmy, M.H.

(2002)

Development of an improved method for production of

antiscorpion F(ab')2 fragment of IgG with high yield and potency.

J. Nat. Toxins, 11(2):123-32.

Salwa, S. A.; Wanas, S.; Abdel Malak, G. and Helmy, M. H. (2003).

Efficacy of IgG, Fab, and F(ab')2 fragments of horse antivenom in

the treatment of local symptoms after Cerastes cerastes (Egyptian

snake) bite.

Afr. J. Biotechnol., 2 (7): 189–193.

Schiavo, G.; Benfenati, F.; Poulain, B.; Rossett, O.O.; Shone,C.C.;

Dasgupta, B,R, and Montecucco, C. (1994).

Tetanus and botulinum neurotoxins are zinc proteases specific for

proteins involved in vesicle docking and fusion. In: Bacterial

Protein Toxins (Freer et al. edition) Gustav Fischer, Stuttgart, Jena,

New York.

Sheoran, A. S. and Holmes, A. M. (1996).

Separation of equine IgG subclasses ( IgGa, IgGb and IgG( T) )

using their differential binding characteristics for staphylococcal

protein A and streptococcal protein G.

Vet. Immunol. Immunopathol., 55: 33-43.

Shone, C.C. and Tranter, H.S.(1995).

Tetanus neurotoxin In: Clostridial Neurotoxins. The Molecular

Pathogenesis of Tetanus and Botulism. Montecucco C. (Ed.),

Springer-Verlag, Berlin.

REFERENCES

﴿ ﴾

134

Slade, A.L; Schoeniger,J.S; Darryl Y; Sasaki, z and Yip, C M.(2006).

In Situ Scanning Probe Microscopy Studies of Tetanus Toxin-

Membrane Interactions.

Biophysical J., 91: 4565–4574.

Smith, J.W.G.and MacIver, A.G. (1975)

Growth of Clostridium tetani in vivo.

Progresss Drug Research, 19: 384-390

Smith, D.C.; Reddi, K.R.; Laing, G.; Theakstone, R.G. and Landon,

J. (1992).

An affinity purified ovine antivenome for the treatment of Vipera

berus envenoming.

Toxicon, 30: 865-871.

Teng, Q.; Tanase, D.; Liu, J.K.; Garrity-Moses, M.E.; Baker, K.B.

and Boulis, N.M. (2005).

Adenoviral clostridial light chain gene-based synaptic inhibition

through neuronal synaptobrevin elimination.

Gene. Ther., 12(2): 108-119.

Thomson, R.O. (1957).

A semi-continuous method for the large-scale production of tetanus toxin.

Nature, 180: 1126-1127.

Tregoning, J.S.; Clare, S.; Bowe, F.; Edwards, L.; Fairweather, N.;

Qazi, O.; Nixon, P.J.; Maliga, P.; Dougan, G. and Hussell, T. (2005).

Protection against tetanus toxin using a plant-based vaccine.

Eur. J. Immunol., 35(4): 1320-6.

REFERENCES

﴿ ﴾

135

Turillazzi, E.; Neri, M.; Pomara, C.; Riezzo, I. and Fineschi, V.

(2009).

An immunohistochemical study on a tetanus fatal case using toxin

fragment C (TTC). Should it be a useful diagnostic tool?

Neuropathology, 29(1):68-71.

Umland, T.C; Wingert, L; Swaminathan, S; Schmidt, J.J. and Sax,

M.(1998)

Crystallization and preliminary X-ray analysis of tetanus

neurotoxin C fragment.

Acta Crystallogr D Biol Crystallogr. 54 (Pt 2): 273-5.

Wafaa, R. A. (2005).

Advancing studies on Clostridium tetani.

M.V.Sc. Thesis, Microbiology (Bacteriology,Immunology and

mycology), Fac. of Vet. Med., Moshtohor, Benha Univ.

Walker, J.M.(2002)

The Bradford Method for Protein Quantitation In: The protein

Protocols Handbook. 2nd Edition, Humana Press, Totowa, New

Jersey.

Wang L.; Sun, X. and Ghosh, R. (2008)

Purification of equine IgG using membrane based enhanced hybrid

bioseparation technique: a potential method for manufacturing

hyperimmune antibody.

Biotechnol. Bioeng., 99(3): 625-33.

REFERENCES

﴿ ﴾

136

Wang, L.; Khan, T.; Mohanty, K. and Ghosh, R. (2010).

Cascade ultrafiltration bioreactor-separator system for continuous

production of F(ab)2 fragment from immunoglobulin G.

J. Memb. Sci., 351: 96–103

Watt, B. and Brown, R. (1975).

A defined medium for the growth of Clostridium tetani and other

anaerobes of clinical interest.

J. Med. Microbiol., 8: 167-172.

Weller, U.; Dauzenroth, M.E.; Meyer, z.u; Heringdorf, D. and

Habermann, E. (1989)

Chains and fragments of tetanus toxin. Separation, reassociation

and pharmacological properties.

Eur. J. Biochem., 182(3): 649-56.

Williamson, L.C. and Neale, A. E. (1994)

Tetanus toxin enters neurons through acidic endosomes: Studies in

spinal cord cell cultures. In: Bacterial protein toxins (Freer et al.

Edition) Gustav Fischer, Stuttgart, Jena, NewYork.

Williamson, L.C; Bateman, K.E.; Clifford, J.C.M. and Neale, A.E.

(1999).

Neuronal sensitivity to tetanus toxin requires ganglioside

J. Biol. Chem., 274: 25173-25180.