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Successful R&I in Europe 2015 Lars M. Blank 06.11.2015 From Plastic waste to Plastic value using Pseudomonas putida Synthetic Biology

From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

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Page 1: From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

Successful R&I in Europe 2015

Lars M. Blank

06.11.2015

From Plastic waste to Plastic value

using Pseudomonas putida

Synthetic Biology

Page 2: From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

The P4SB coordinators

Lars Blank – RWTH | iAMB

Coordinator

Scientific

Nick Wierckx – RWTH | iAMB

Co-coordinator

Scientific

Christine Kempchen – RWTH | Div. 4.2

Project manager

Administration, organization, finance

Page 3: From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

The Consortium

Participant Prinicipal

investigators Country Expertise

RWTH Aachen

(coordinator)

Lars Blank, Nick

Wierckx DE

Metabolic

engineering

University Leipzig Wolfgang

Zimmermann DE PET-hydrolysis

CSIC – National

Centre for

Biotechnology

Auxi Prieto, Juan

Nogales ES

PHA-biotechnology;

Model-based design

University College

Dublin Kevin O’Connor IE PET-biotechnology

Bioplastech Shane Kenny IE PHA-production

Bacmine Pablo Pomposiello ES Synthetic Biology

Helmholz Centre for

Environmental

Research

Hermann Heipieper DE Bacterial stress

response

University of Surrey José Jiminez UK Synthetic Biology

CNRS – University of

Strasbourg

Luc Averous, Eric

Pollet FR PU-hydrolysis

Soprema Rémi Perrin FR Plastic manufacturer

Protéus Cécile Persillon FR Enzyme technology

Page 4: From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

The Scientific Advisory Board

Participant affiliation

Prof. Ken Timmis TU Braunschweig

Prof. Sven Panke ETH Zürich

Dr. Hans-Christian

Schäfer

German Federal

Environmental Foundation

(DBU)

Prof. Vitor martin dos

Santos

Wageningen University

(EmPowerPutida)

Prof. Luis Serrano Pubul

Center for Genomic

Regulation

(MycoSynVac)

Dr. Carmen de Vincente

Coll European Commission

Pre-invited

BIOTEC-1-2014

coordinators

Research programme

officer

Page 5: From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

5

Page 6: From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

The problem

Plastic waste!

Best case:

Worst case:

pixelo.de

oceanwire.wordpress.com pinterest.com

Page 7: From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

The problem

8 million tons per year into the oceans!

175 million metric tons

global sugar production

source: USDA.gov

Page 8: From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

The solution?

Recycling! – PET

Ideal cycle

Page 9: From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

Recycling! – PET

Deteriorating spiral

Less than 30%

is recycled!!!

wikipedia.org

The solution?

Page 10: From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

The solution?

Recycling! – PU?

Thermoset: no re-moulding

Less than 5%

is recycled!!!

Page 11: From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

The solution?

EU recycling targets

EU 2020 target now

PET 50% <30%

PU 70% <5%

EU directive 2008/98/EC

Page 12: From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

The P4SB objective

the biotransformation of non-sustainable plastic waste into

sustainable value-added alternative materials

Bio-upcycling

Bioplastics

Page 13: From Plastic waste to Plastic value using Pseudomonas ......Hermann Heipieper DE Bacterial stress response University of Surrey José Jiminez UK Synthetic Biology CNRS – University

The P4SB approach

Dissemination / Exploitation WP8

Synthetic Biology / Model-based design

plastic

hydrolysis

monomer

metabolism PHA

production

PHA

secretion

WP2 WP3 WP4 WP5

WP6

WP1

WP7