Embed Size (px)
Citation preview
From Epitope Mapping to Autoimmune
Infectious Disease and Vaccine Research
Corporate Presentation 2018
Who we are
History
bull Founded amp based in Heidelberg Germany
bull Privately held independent company
bull Market launch in 2011
bull ISO 9001 certified QMS
Scientific Background
bull Experienced team of executives
bull Proven track record (eg Science Nature)
bull Strong IP protection
Products for Academia amp Pharma
bull Linear amp conformational epitope mapping
bull Serum antibody amp protein profiling
bull Antigen epitope amp peptide discovery
bull Development of IVD amp CDx tests
Customers amp Partners
Area of Activity
Headquarter Sales Representatives Distributors
Key-Product
Laser-Printed Peptide Microarrays
and Analysis Services
Standard
Standard peptide array
Spotting of peptides on chip
Pre-synthesis of peptides
bull High number of different peptides
bull Highly customizable
bull Highly economic
PEPperCHIPreg Peptide Microarray
On-chip peptide synthesis
bull Expensive peptide pre-synthesis
bull Small peptide libraries
bull Mostly standard peptide content
Competitive Advantage
Benefits
bull Very low material consumption
bull High content (1000 peptidescm2)
bull Digital printing flexibility amp fast production times
bull High peptide quality with routine double couplings
Peptide Microarray Technology
75460
peptides
Discovery Format
11280
peptides
Standard Format
5 x 1632
peptides
Mapping Format
PEPperCHIPreg Peptide Microarrays
with Accessories
send to customer
PEPperMAPreg Services
full analysis service
16 x 273
peptides
Multiplexed Format
PEPperCHIPreg Product Portfolio
Structure
Linear amp conformational peptides
Quality
ISO 9001 standards
Flexibility
Easy library maturation
Speed
Library set-up in one week
Size
Biggest antigen libraries
Resolution
Epitope biomarker discovery
Why PEPperPRINT
Why PEPperPRINT
92 of PEPperPRINT customers would recommend us to a colleague
Result of Customer Survey 2017
2
6
92
No
Unsure
Yes
Would you recommend PEPperPRINT to a colleague
hellipread-out a patients immune profile in a day
helliptranslate sequencing data of cancer patients into proteomics
hellipidentify neutralizing antibodies for malaria vaccine research
hellipdifferentiate Zika and Dengue virus infections with a drop of blood
hellipselect the best drug for each patient in a single assay
hellipdramatically improve the efficiency of peptide drugs
PEPperCHIPreg Applications
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
Antibody Analysis
bull linear and conformational epitope mapping service with max epitope resolution
bull including a custom peptide microarray with single or multiple antigens
PEPperMAPreg Epitope Mapping
bull in-depth analysis of epitopes for the identification of essential and variable amino acids
bull ideal solution for specificity andor cross-reactivity analyses of monoclonal antibodies
PEPperMAPreg Epitope Substitution Scans
bull identification of unknown antigens and epitopes with the Human Epitome Microarray
bull cross-reactivity profiling of antibodies
PEPperMAPreg Antibody Cross-Reactivity Analysis
Microarray Scan
(Double Spots)
Data Analysis
Antigen Sequence
Overlapping Peptides
Peptide Microarray
Secondary Antibody
Target Antibody
Monoclonal Sample
Polyclonal Sample
Epitope = Consensus Motif
Epitope Mapping
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Who we are
History
bull Founded amp based in Heidelberg Germany
bull Privately held independent company
bull Market launch in 2011
bull ISO 9001 certified QMS
Scientific Background
bull Experienced team of executives
bull Proven track record (eg Science Nature)
bull Strong IP protection
Products for Academia amp Pharma
bull Linear amp conformational epitope mapping
bull Serum antibody amp protein profiling
bull Antigen epitope amp peptide discovery
bull Development of IVD amp CDx tests
Customers amp Partners
Area of Activity
Headquarter Sales Representatives Distributors
Key-Product
Laser-Printed Peptide Microarrays
and Analysis Services
Standard
Standard peptide array
Spotting of peptides on chip
Pre-synthesis of peptides
bull High number of different peptides
bull Highly customizable
bull Highly economic
PEPperCHIPreg Peptide Microarray
On-chip peptide synthesis
bull Expensive peptide pre-synthesis
bull Small peptide libraries
bull Mostly standard peptide content
Competitive Advantage
Benefits
bull Very low material consumption
bull High content (1000 peptidescm2)
bull Digital printing flexibility amp fast production times
bull High peptide quality with routine double couplings
Peptide Microarray Technology
75460
peptides
Discovery Format
11280
peptides
Standard Format
5 x 1632
peptides
Mapping Format
PEPperCHIPreg Peptide Microarrays
with Accessories
send to customer
PEPperMAPreg Services
full analysis service
16 x 273
peptides
Multiplexed Format
PEPperCHIPreg Product Portfolio
Structure
Linear amp conformational peptides
Quality
ISO 9001 standards
Flexibility
Easy library maturation
Speed
Library set-up in one week
Size
Biggest antigen libraries
Resolution
Epitope biomarker discovery
Why PEPperPRINT
Why PEPperPRINT
92 of PEPperPRINT customers would recommend us to a colleague
Result of Customer Survey 2017
2
6
92
No
Unsure
Yes
Would you recommend PEPperPRINT to a colleague
hellipread-out a patients immune profile in a day
helliptranslate sequencing data of cancer patients into proteomics
hellipidentify neutralizing antibodies for malaria vaccine research
hellipdifferentiate Zika and Dengue virus infections with a drop of blood
hellipselect the best drug for each patient in a single assay
hellipdramatically improve the efficiency of peptide drugs
PEPperCHIPreg Applications
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
Antibody Analysis
bull linear and conformational epitope mapping service with max epitope resolution
bull including a custom peptide microarray with single or multiple antigens
PEPperMAPreg Epitope Mapping
bull in-depth analysis of epitopes for the identification of essential and variable amino acids
bull ideal solution for specificity andor cross-reactivity analyses of monoclonal antibodies
PEPperMAPreg Epitope Substitution Scans
bull identification of unknown antigens and epitopes with the Human Epitome Microarray
bull cross-reactivity profiling of antibodies
PEPperMAPreg Antibody Cross-Reactivity Analysis
Microarray Scan
(Double Spots)
Data Analysis
Antigen Sequence
Overlapping Peptides
Peptide Microarray
Secondary Antibody
Target Antibody
Monoclonal Sample
Polyclonal Sample
Epitope = Consensus Motif
Epitope Mapping
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Area of Activity
Headquarter Sales Representatives Distributors
Key-Product
Laser-Printed Peptide Microarrays
and Analysis Services
Standard
Standard peptide array
Spotting of peptides on chip
Pre-synthesis of peptides
bull High number of different peptides
bull Highly customizable
bull Highly economic
PEPperCHIPreg Peptide Microarray
On-chip peptide synthesis
bull Expensive peptide pre-synthesis
bull Small peptide libraries
bull Mostly standard peptide content
Competitive Advantage
Benefits
bull Very low material consumption
bull High content (1000 peptidescm2)
bull Digital printing flexibility amp fast production times
bull High peptide quality with routine double couplings
Peptide Microarray Technology
75460
peptides
Discovery Format
11280
peptides
Standard Format
5 x 1632
peptides
Mapping Format
PEPperCHIPreg Peptide Microarrays
with Accessories
send to customer
PEPperMAPreg Services
full analysis service
16 x 273
peptides
Multiplexed Format
PEPperCHIPreg Product Portfolio
Structure
Linear amp conformational peptides
Quality
ISO 9001 standards
Flexibility
Easy library maturation
Speed
Library set-up in one week
Size
Biggest antigen libraries
Resolution
Epitope biomarker discovery
Why PEPperPRINT
Why PEPperPRINT
92 of PEPperPRINT customers would recommend us to a colleague
Result of Customer Survey 2017
2
6
92
No
Unsure
Yes
Would you recommend PEPperPRINT to a colleague
hellipread-out a patients immune profile in a day
helliptranslate sequencing data of cancer patients into proteomics
hellipidentify neutralizing antibodies for malaria vaccine research
hellipdifferentiate Zika and Dengue virus infections with a drop of blood
hellipselect the best drug for each patient in a single assay
hellipdramatically improve the efficiency of peptide drugs
PEPperCHIPreg Applications
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
Antibody Analysis
bull linear and conformational epitope mapping service with max epitope resolution
bull including a custom peptide microarray with single or multiple antigens
PEPperMAPreg Epitope Mapping
bull in-depth analysis of epitopes for the identification of essential and variable amino acids
bull ideal solution for specificity andor cross-reactivity analyses of monoclonal antibodies
PEPperMAPreg Epitope Substitution Scans
bull identification of unknown antigens and epitopes with the Human Epitome Microarray
bull cross-reactivity profiling of antibodies
PEPperMAPreg Antibody Cross-Reactivity Analysis
Microarray Scan
(Double Spots)
Data Analysis
Antigen Sequence
Overlapping Peptides
Peptide Microarray
Secondary Antibody
Target Antibody
Monoclonal Sample
Polyclonal Sample
Epitope = Consensus Motif
Epitope Mapping
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Key-Product
Laser-Printed Peptide Microarrays
and Analysis Services
Standard
Standard peptide array
Spotting of peptides on chip
Pre-synthesis of peptides
bull High number of different peptides
bull Highly customizable
bull Highly economic
PEPperCHIPreg Peptide Microarray
On-chip peptide synthesis
bull Expensive peptide pre-synthesis
bull Small peptide libraries
bull Mostly standard peptide content
Competitive Advantage
Benefits
bull Very low material consumption
bull High content (1000 peptidescm2)
bull Digital printing flexibility amp fast production times
bull High peptide quality with routine double couplings
Peptide Microarray Technology
75460
peptides
Discovery Format
11280
peptides
Standard Format
5 x 1632
peptides
Mapping Format
PEPperCHIPreg Peptide Microarrays
with Accessories
send to customer
PEPperMAPreg Services
full analysis service
16 x 273
peptides
Multiplexed Format
PEPperCHIPreg Product Portfolio
Structure
Linear amp conformational peptides
Quality
ISO 9001 standards
Flexibility
Easy library maturation
Speed
Library set-up in one week
Size
Biggest antigen libraries
Resolution
Epitope biomarker discovery
Why PEPperPRINT
Why PEPperPRINT
92 of PEPperPRINT customers would recommend us to a colleague
Result of Customer Survey 2017
2
6
92
No
Unsure
Yes
Would you recommend PEPperPRINT to a colleague
hellipread-out a patients immune profile in a day
helliptranslate sequencing data of cancer patients into proteomics
hellipidentify neutralizing antibodies for malaria vaccine research
hellipdifferentiate Zika and Dengue virus infections with a drop of blood
hellipselect the best drug for each patient in a single assay
hellipdramatically improve the efficiency of peptide drugs
PEPperCHIPreg Applications
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
Antibody Analysis
bull linear and conformational epitope mapping service with max epitope resolution
bull including a custom peptide microarray with single or multiple antigens
PEPperMAPreg Epitope Mapping
bull in-depth analysis of epitopes for the identification of essential and variable amino acids
bull ideal solution for specificity andor cross-reactivity analyses of monoclonal antibodies
PEPperMAPreg Epitope Substitution Scans
bull identification of unknown antigens and epitopes with the Human Epitome Microarray
bull cross-reactivity profiling of antibodies
PEPperMAPreg Antibody Cross-Reactivity Analysis
Microarray Scan
(Double Spots)
Data Analysis
Antigen Sequence
Overlapping Peptides
Peptide Microarray
Secondary Antibody
Target Antibody
Monoclonal Sample
Polyclonal Sample
Epitope = Consensus Motif
Epitope Mapping
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Standard
Standard peptide array
Spotting of peptides on chip
Pre-synthesis of peptides
bull High number of different peptides
bull Highly customizable
bull Highly economic
PEPperCHIPreg Peptide Microarray
On-chip peptide synthesis
bull Expensive peptide pre-synthesis
bull Small peptide libraries
bull Mostly standard peptide content
Competitive Advantage
Benefits
bull Very low material consumption
bull High content (1000 peptidescm2)
bull Digital printing flexibility amp fast production times
bull High peptide quality with routine double couplings
Peptide Microarray Technology
75460
peptides
Discovery Format
11280
peptides
Standard Format
5 x 1632
peptides
Mapping Format
PEPperCHIPreg Peptide Microarrays
with Accessories
send to customer
PEPperMAPreg Services
full analysis service
16 x 273
peptides
Multiplexed Format
PEPperCHIPreg Product Portfolio
Structure
Linear amp conformational peptides
Quality
ISO 9001 standards
Flexibility
Easy library maturation
Speed
Library set-up in one week
Size
Biggest antigen libraries
Resolution
Epitope biomarker discovery
Why PEPperPRINT
Why PEPperPRINT
92 of PEPperPRINT customers would recommend us to a colleague
Result of Customer Survey 2017
2
6
92
No
Unsure
Yes
Would you recommend PEPperPRINT to a colleague
hellipread-out a patients immune profile in a day
helliptranslate sequencing data of cancer patients into proteomics
hellipidentify neutralizing antibodies for malaria vaccine research
hellipdifferentiate Zika and Dengue virus infections with a drop of blood
hellipselect the best drug for each patient in a single assay
hellipdramatically improve the efficiency of peptide drugs
PEPperCHIPreg Applications
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
Antibody Analysis
bull linear and conformational epitope mapping service with max epitope resolution
bull including a custom peptide microarray with single or multiple antigens
PEPperMAPreg Epitope Mapping
bull in-depth analysis of epitopes for the identification of essential and variable amino acids
bull ideal solution for specificity andor cross-reactivity analyses of monoclonal antibodies
PEPperMAPreg Epitope Substitution Scans
bull identification of unknown antigens and epitopes with the Human Epitome Microarray
bull cross-reactivity profiling of antibodies
PEPperMAPreg Antibody Cross-Reactivity Analysis
Microarray Scan
(Double Spots)
Data Analysis
Antigen Sequence
Overlapping Peptides
Peptide Microarray
Secondary Antibody
Target Antibody
Monoclonal Sample
Polyclonal Sample
Epitope = Consensus Motif
Epitope Mapping
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Benefits
bull Very low material consumption
bull High content (1000 peptidescm2)
bull Digital printing flexibility amp fast production times
bull High peptide quality with routine double couplings
Peptide Microarray Technology
75460
peptides
Discovery Format
11280
peptides
Standard Format
5 x 1632
peptides
Mapping Format
PEPperCHIPreg Peptide Microarrays
with Accessories
send to customer
PEPperMAPreg Services
full analysis service
16 x 273
peptides
Multiplexed Format
PEPperCHIPreg Product Portfolio
Structure
Linear amp conformational peptides
Quality
ISO 9001 standards
Flexibility
Easy library maturation
Speed
Library set-up in one week
Size
Biggest antigen libraries
Resolution
Epitope biomarker discovery
Why PEPperPRINT
Why PEPperPRINT
92 of PEPperPRINT customers would recommend us to a colleague
Result of Customer Survey 2017
2
6
92
No
Unsure
Yes
Would you recommend PEPperPRINT to a colleague
hellipread-out a patients immune profile in a day
helliptranslate sequencing data of cancer patients into proteomics
hellipidentify neutralizing antibodies for malaria vaccine research
hellipdifferentiate Zika and Dengue virus infections with a drop of blood
hellipselect the best drug for each patient in a single assay
hellipdramatically improve the efficiency of peptide drugs
PEPperCHIPreg Applications
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
Antibody Analysis
bull linear and conformational epitope mapping service with max epitope resolution
bull including a custom peptide microarray with single or multiple antigens
PEPperMAPreg Epitope Mapping
bull in-depth analysis of epitopes for the identification of essential and variable amino acids
bull ideal solution for specificity andor cross-reactivity analyses of monoclonal antibodies
PEPperMAPreg Epitope Substitution Scans
bull identification of unknown antigens and epitopes with the Human Epitome Microarray
bull cross-reactivity profiling of antibodies
PEPperMAPreg Antibody Cross-Reactivity Analysis
Microarray Scan
(Double Spots)
Data Analysis
Antigen Sequence
Overlapping Peptides
Peptide Microarray
Secondary Antibody
Target Antibody
Monoclonal Sample
Polyclonal Sample
Epitope = Consensus Motif
Epitope Mapping
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
75460
peptides
Discovery Format
11280
peptides
Standard Format
5 x 1632
peptides
Mapping Format
PEPperCHIPreg Peptide Microarrays
with Accessories
send to customer
PEPperMAPreg Services
full analysis service
16 x 273
peptides
Multiplexed Format
PEPperCHIPreg Product Portfolio
Structure
Linear amp conformational peptides
Quality
ISO 9001 standards
Flexibility
Easy library maturation
Speed
Library set-up in one week
Size
Biggest antigen libraries
Resolution
Epitope biomarker discovery
Why PEPperPRINT
Why PEPperPRINT
92 of PEPperPRINT customers would recommend us to a colleague
Result of Customer Survey 2017
2
6
92
No
Unsure
Yes
Would you recommend PEPperPRINT to a colleague
hellipread-out a patients immune profile in a day
helliptranslate sequencing data of cancer patients into proteomics
hellipidentify neutralizing antibodies for malaria vaccine research
hellipdifferentiate Zika and Dengue virus infections with a drop of blood
hellipselect the best drug for each patient in a single assay
hellipdramatically improve the efficiency of peptide drugs
PEPperCHIPreg Applications
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
Antibody Analysis
bull linear and conformational epitope mapping service with max epitope resolution
bull including a custom peptide microarray with single or multiple antigens
PEPperMAPreg Epitope Mapping
bull in-depth analysis of epitopes for the identification of essential and variable amino acids
bull ideal solution for specificity andor cross-reactivity analyses of monoclonal antibodies
PEPperMAPreg Epitope Substitution Scans
bull identification of unknown antigens and epitopes with the Human Epitome Microarray
bull cross-reactivity profiling of antibodies
PEPperMAPreg Antibody Cross-Reactivity Analysis
Microarray Scan
(Double Spots)
Data Analysis
Antigen Sequence
Overlapping Peptides
Peptide Microarray
Secondary Antibody
Target Antibody
Monoclonal Sample
Polyclonal Sample
Epitope = Consensus Motif
Epitope Mapping
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Structure
Linear amp conformational peptides
Quality
ISO 9001 standards
Flexibility
Easy library maturation
Speed
Library set-up in one week
Size
Biggest antigen libraries
Resolution
Epitope biomarker discovery
Why PEPperPRINT
Why PEPperPRINT
92 of PEPperPRINT customers would recommend us to a colleague
Result of Customer Survey 2017
2
6
92
No
Unsure
Yes
Would you recommend PEPperPRINT to a colleague
hellipread-out a patients immune profile in a day
helliptranslate sequencing data of cancer patients into proteomics
hellipidentify neutralizing antibodies for malaria vaccine research
hellipdifferentiate Zika and Dengue virus infections with a drop of blood
hellipselect the best drug for each patient in a single assay
hellipdramatically improve the efficiency of peptide drugs
PEPperCHIPreg Applications
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
Antibody Analysis
bull linear and conformational epitope mapping service with max epitope resolution
bull including a custom peptide microarray with single or multiple antigens
PEPperMAPreg Epitope Mapping
bull in-depth analysis of epitopes for the identification of essential and variable amino acids
bull ideal solution for specificity andor cross-reactivity analyses of monoclonal antibodies
PEPperMAPreg Epitope Substitution Scans
bull identification of unknown antigens and epitopes with the Human Epitome Microarray
bull cross-reactivity profiling of antibodies
PEPperMAPreg Antibody Cross-Reactivity Analysis
Microarray Scan
(Double Spots)
Data Analysis
Antigen Sequence
Overlapping Peptides
Peptide Microarray
Secondary Antibody
Target Antibody
Monoclonal Sample
Polyclonal Sample
Epitope = Consensus Motif
Epitope Mapping
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Why PEPperPRINT
92 of PEPperPRINT customers would recommend us to a colleague
Result of Customer Survey 2017
2
6
92
No
Unsure
Yes
Would you recommend PEPperPRINT to a colleague
hellipread-out a patients immune profile in a day
helliptranslate sequencing data of cancer patients into proteomics
hellipidentify neutralizing antibodies for malaria vaccine research
hellipdifferentiate Zika and Dengue virus infections with a drop of blood
hellipselect the best drug for each patient in a single assay
hellipdramatically improve the efficiency of peptide drugs
PEPperCHIPreg Applications
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
Antibody Analysis
bull linear and conformational epitope mapping service with max epitope resolution
bull including a custom peptide microarray with single or multiple antigens
PEPperMAPreg Epitope Mapping
bull in-depth analysis of epitopes for the identification of essential and variable amino acids
bull ideal solution for specificity andor cross-reactivity analyses of monoclonal antibodies
PEPperMAPreg Epitope Substitution Scans
bull identification of unknown antigens and epitopes with the Human Epitome Microarray
bull cross-reactivity profiling of antibodies
PEPperMAPreg Antibody Cross-Reactivity Analysis
Microarray Scan
(Double Spots)
Data Analysis
Antigen Sequence
Overlapping Peptides
Peptide Microarray
Secondary Antibody
Target Antibody
Monoclonal Sample
Polyclonal Sample
Epitope = Consensus Motif
Epitope Mapping
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
hellipread-out a patients immune profile in a day
helliptranslate sequencing data of cancer patients into proteomics
hellipidentify neutralizing antibodies for malaria vaccine research
hellipdifferentiate Zika and Dengue virus infections with a drop of blood
hellipselect the best drug for each patient in a single assay
hellipdramatically improve the efficiency of peptide drugs
PEPperCHIPreg Applications
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
Antibody Analysis
bull linear and conformational epitope mapping service with max epitope resolution
bull including a custom peptide microarray with single or multiple antigens
PEPperMAPreg Epitope Mapping
bull in-depth analysis of epitopes for the identification of essential and variable amino acids
bull ideal solution for specificity andor cross-reactivity analyses of monoclonal antibodies
PEPperMAPreg Epitope Substitution Scans
bull identification of unknown antigens and epitopes with the Human Epitome Microarray
bull cross-reactivity profiling of antibodies
PEPperMAPreg Antibody Cross-Reactivity Analysis
Microarray Scan
(Double Spots)
Data Analysis
Antigen Sequence
Overlapping Peptides
Peptide Microarray
Secondary Antibody
Target Antibody
Monoclonal Sample
Polyclonal Sample
Epitope = Consensus Motif
Epitope Mapping
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
Antibody Analysis
bull linear and conformational epitope mapping service with max epitope resolution
bull including a custom peptide microarray with single or multiple antigens
PEPperMAPreg Epitope Mapping
bull in-depth analysis of epitopes for the identification of essential and variable amino acids
bull ideal solution for specificity andor cross-reactivity analyses of monoclonal antibodies
PEPperMAPreg Epitope Substitution Scans
bull identification of unknown antigens and epitopes with the Human Epitome Microarray
bull cross-reactivity profiling of antibodies
PEPperMAPreg Antibody Cross-Reactivity Analysis
Microarray Scan
(Double Spots)
Data Analysis
Antigen Sequence
Overlapping Peptides
Peptide Microarray
Secondary Antibody
Target Antibody
Monoclonal Sample
Polyclonal Sample
Epitope = Consensus Motif
Epitope Mapping
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Antibody Analysis
bull linear and conformational epitope mapping service with max epitope resolution
bull including a custom peptide microarray with single or multiple antigens
PEPperMAPreg Epitope Mapping
bull in-depth analysis of epitopes for the identification of essential and variable amino acids
bull ideal solution for specificity andor cross-reactivity analyses of monoclonal antibodies
PEPperMAPreg Epitope Substitution Scans
bull identification of unknown antigens and epitopes with the Human Epitome Microarray
bull cross-reactivity profiling of antibodies
PEPperMAPreg Antibody Cross-Reactivity Analysis
Microarray Scan
(Double Spots)
Data Analysis
Antigen Sequence
Overlapping Peptides
Peptide Microarray
Secondary Antibody
Target Antibody
Monoclonal Sample
Polyclonal Sample
Epitope = Consensus Motif
Epitope Mapping
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Microarray Scan
(Double Spots)
Data Analysis
Antigen Sequence
Overlapping Peptides
Peptide Microarray
Secondary Antibody
Target Antibody
Monoclonal Sample
Polyclonal Sample
Epitope = Consensus Motif
Epitope Mapping
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Possible Samples
bull monoclonal or polyclonal antibodies
bull synthetic or purified antibodies hybridoma
supernatant
bull plasma or serum samples
bull species human mouse rabbit goat
chicken pig bovinehellip
bull isotypes total Ig IgG IgM IgE IgY IgAhellip
PEPperPRINT uses a maximum
peptide-peptide overlap (shift 1)
to get high-resolution
epitope data
Epitope Mapping
Antigen Sequence
Overlapping Peptides
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
bull clear separation of two adjacent epitopes
bull easy determination of epitope length
bull exact identification of conserved core motifs
Length 15 aa Overlap 14 aa (Shift 1)
YES
Length 15 aa Overlap 11 aa (Shift 4)
Competitor
NO
Epitope Mapping
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
bull model therapeutic mAb Rituximab directed against the extracellular domain of human CD20
bull data basis X-ray Analysis of a co-crystal of Rituximab Fab fragment and CD20 peptide (163- 187 aa)
bull observation conformational epitope due to disulfide bond formation
bull outcome 15 amino acid loop NIYNCEPANPSEKNSPSTQYCYSIQ covers the epitope of Rituximab
Conformational Epitope Mapping
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
bull goal epitope mapping of Rituximab (Rituxanreg) a chimeric monoclonal anti-CD20 antibody
bull microarray CD20 as linear and cyclic constrained overlapping peptides (7 aa 10 aa and 13 aa)
bull sample monoclonal antibody Rituximab
bull outcome high resolution data with EPANPSEK as Rituximab epitope
Rituximab 100 microgml
Rituximab 100 microgml
No response
Cyclized at 7aa 10aa and 13aa
Epitope EPANPSEK (8aa)
Linear peptides
Conformational Epitope Mapping
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
bull goal in-depth epitope analysis to determine conserved and variable amino acids
bull microarray setup full substitution scan of looped peptide NIYNCEPANPSEK with all 20 amino acids
bull data analysis antibody binding relative to the wild type epitope at 100
bull outcome detailed information about essential conserved and variable amino acids
Microarray Scan
Heatmap Part of extracellular domain of CD20
Epitope
Essential
Sig
nal in
tensity
(wild
typ
e e
pitope s
et to
100
)
Epitope Substitution Scan
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Applications
bull epitope mapping and analysis of therapeutic
antibodies
bull cross-reactivity analysis with response matrix
bull patient stratification point mutations vs
essential amino acids
bull specificity testing of diagnostic antibodies
Epitope Substitution Scan
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Content
bull covers all human epitopes of the
Immune Epitope Database (wwwiedborg)
8504 epitopes of infectious diseases
3808 epitopes of autoimmune diseases
4661 epitopes of the most common
vaccines
199 cancer epitopes 3451 epitopes of
allergens
28895 different peptides of 2542
proteins and 468 organisms
Applications
bull epitome-wide antibody screening
bull differentiation between IgG and IgM or IgA
response profiles
bull high throughput serum biomarker discovery
bull identification of prognostic epitopes
bull cross-reactivity analysis of mono- and
polyclonal antibodies
bull patient stratification
bull investigation of links between autoimmune
and infectious diseases
bull cross-reactivity analysis of therapeutic and
diagnostic antibodies
PEPperCHIPreg Human Epitome Microarray
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Human Monoclonal Antibody anti-c-Myc (chi9E10) 2 microgml
bull microarray setup PEPperCHIPreg
Human Epitome Microarray
bull goal cross-reactivity analysis with
28895 human database epitopes on
one chip
bull sample chimeric human anti-Myc
(chi9E10) antibody
bull data analysis few but clear peptide
hits (red) with 2 microgml antibody
bull outcome cross-reactions against
epitopes of nucleoprotein
erythrocyte membrane-associated
giant protein antigen 332 and
dihydrolipoamide S-
acetyltransferase
One-Step Cross-Reactivity Analysis
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Peptide anti-c-Myc (chi9E10) 2 microgml Epitope ID Source Molecule Accession Source Molecule Name Source Organism ID Source Organism Name
LGITAEDARLVSEIAMH 79875 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
EEVVGEEKLVSEEIVT 25350 11927 13508497 Erythrocyte Membrane-Associated Giant Protein Antigen 332 5833 Plasmodium Falciparum
EVQEEGYLAKILVPE 19605 125443 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
LAKILVPEGTRDVP 15590 125468 119587578 Dihydrolipoamide S-Acetyltransferase (E2 Component Of Pyruvate Dehydrogenase Complex) Isoform Cra_A9606 Homo Sapiens
TAEDARLVSEIAMHTTE 10505 98874 127900 Nucleoprotein 11235 Measles virus strain Edmonston
TYGRKLHLYSHPIILGF 10075 190568 4323200 Polymerase 10407 Hepatitis B virus
ALVAEGIEAIVFRTL 9230 178535 15608176 Truncated Is1560 Transposase 1773 Mycobacterium tuberculosis
NGFLDVFTSFGGLVAE 7480 6520 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
FTSFGGLVAEAFGF 6390 18069 75352214 Outer Surface Protein Vlse 224326 Borrelia Burgdorferi B31
QYLVGERTVLAGQCYI 6200 133331 113118 Muscarinic Acetylcholine Receptor M1 9606 Homo Sapiens
AQTQSLVYPF 5930 78152 162797 Beta-Casein Precursor 9913 Bos Taurus
VVSYVNTNVGLKFRQLL 5475 53394 164509170 Precore Protein 10418 Hepatitis B virus subtype ayw
VLNPWDQVKR 5455 95970 115654 Alpha-S2-Casein Precursor 9913 Bos Taurus
WLSLLVPFV 4960 72794 15211906 Large Surface Antigen 10407 Hepatitis B virus
GRSPRRRTPSPRRR 4950 55713 16930336 Core Protein 10407 Hepatitis B virus
GLSPTVWLSV 4850 21139 128168864 Envelope Protein 10407 Hepatitis B virus
PSPRRRRSQSPRRRR 4755 55713 16930336 Core Protein 10407 Hepatitis B virus
WSEGEGAVFYRVDLHFI 4680 119822 62094 110 Kd Polyprotein Precursor 11041 Rubella virus
GAAGTAAQAAVVRFQ 4430 21976 15611010 10 Kda Culture Filtrate Antigen Esxb 1773 Mycobacterium tuberculosis
PWATLVAES 4180 140582
KYGGTEIKYNGEEYLI 4140 34437 116200 10 Kda Chaperonin 1773 Mycobacterium tuberculosis
RKLHLYSHPIILGFRKI 4120 190568 4323200 Polymerase 10407 Hepatitis B virus
SSLRGF 4080 156460
MININIFMRESSRSFL 3990 11711
RRRSQSPRRRR 3965 55713 16930336 Core Protein 10407 Hepatitis B virus
HVYLDTVVLLGALAN 3830 25123 15609123 Probable Conserved Integral Membrane Protein 83332 Mycobacterium tuberculosis H37Rv
RIRRSILPYGDSMDRI 3770 125882 146345399 Collagen Alpha-1(Xvii) Chain 9606 Homo Sapiens
WWARRRRRWRRWKRR 3600 127897 5441235 Orf1 68887 Torque Teno Virus
FPGGGQIVGGVYVLPRR 3570 150483 974351 Core Protein 356114 Hepatitis C Virus Genotype 3
LVAEEDER 3525 7979 SRC279960 Genome Polyprotein 11103 Hepatitis C Virus
DVKFPGGGQI 3320 10636 130461 Genome Polyprotein 11103 Hepatitis C Virus
GPSVFLF 3310 107421 494350 Chain H Three-Dimensional Structure Of A Human Immunoglobulin With A Hinge Deletion9606 Homo Sapiens
AIAEYERSAAVLVRYPF 3295 193035 564602885 Peptidylarginine Deiminase 837 Porphyromonas Gingivalis
NPGLLRFLPQLSERL 3240 179310 15609704 Hypothetical Protein 1773 Mycobacterium tuberculosis
FLPSDFFPSV 3115 79531 116946 Capsid Protein 10418 Hepatitis B virus Subtype Ayw
ELGGKPALVPDRQVLYQ 3110 20145 81992797 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
VFCVQP 3110 68440 130458 Genome Polyprotein 31647 Hepatitis C Virus Subtype 1B
QSLSFDSSNPEYFDGYW 3090 176618 226694183 Integrin Alpha-Iib 9606 Homo Sapiens
AQLLTEFAI 3080 4002 57117045 Ppe Family Protein 83332 Mycobacterium tuberculosis H37Rv
CLLCAYSIEF 3015 7713 124757 Ovomucoid Precursor 9031 Gallus Gallus
bull cross-reactivity profile over 28895 known human epitopes with database annotations
bull identification of conserved and variable amino acid positions by MEME motif discovery
One-Step Cross-Reactivity Analysis
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Service Process Overview ndash Part 1
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Service Process Overview ndash Part 2
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
PEPperCHIPreg Autoimmune Epitope Microarray
bull covering 4287 linear autoimmune epitopes of the Immune Epitope Database
bull including 286 citrullinated peptides and the corresponding arginine controls
Autoimmune Research
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Lupus Microarray
bull 28 lupus antigens translated into overlapping peptides for a multiplexed epitope mapping
bull including 761 lupus epitopes of the Immune Epitope Database and literature
PEPperCHIPreg Cyclic Citrullinated Peptide Microarray
bull covering 577 different cyclic peptides of the Immune Epitope Database and literature
bull including 337 different cyclic citrullinated peptides and the corresponding arginine controls
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
PEPperCHIPreg Infectious Disease Epitope Microarray
bull covering 3857 linear infectious disease epitopes of the Immune Epitope Database
bull epitopes of 192 different pathogens including bacterial fungal parasitic and viral pathogens
PEPperCHIPreg Human Epitome Microarray
bull covering all linear human B-cell epitopes of the Immune Epitope Database
bull most comprehensive human epitope microarray with 28895 different peptides
PEPperCHIPreg Standard Peptide Microarrays
bull PEPperCHIPreg Dengue Virus Proteome Microarrays
bull PEPperCHIP reg MERS-CoV Proteome Microarray
bull PEPperCHIP reg Influenza Virus H1N1 Proteome Microarray
bull PEPperCHIP reg Oncovirus Epitope Microarray and many morehellip
Infectious Disease Research
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
PEPperCHIPreg Oncovirus Epitope Microarray
bull covering 3653 linear B-cell epitopes of human oncoviruses
bull including epitopes of hepatitis B and C human herpesviruses human papillomavirus etc
PEPperCHIPreg Tumor Antigen Microarray
bull covering 22 selected tumor antigens as overlapping peptides (p53 NY-ESO-1 etc)
bull multiplexed and isotype-specific epitope mappings against general tumor antigens
PEPperCHIPreg Melanoma Microarray
bull covering 21 melanoma-associated antigens as overlapping peptides (eg MAGEA1 2 3)
bull multiplexed and isotype-specific epitope mappings against melanoma antigens
PEPperCHIPreg Custom Peptide Microarrays
bull covering any epitope or antigen collection with linear or cyclic constrained peptides
bull isotype-specific screening of antibody responses against linear or conformational epitopes
Cancer Reseach
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Cancer Neoepitope Microarrays
Select neoepitopes
from databases amp
sequencing
Translate point
mutations into peptide
microarray
Incubate sera from
cancer patients amp
healthy controls
Identify patient
specific cancer
antibody profiles
Translate cancer point mutations into peptide microarrays
Applications
bull Validate therapeutic antibodies against tumor associated antigens
bull Target discovery Identify immunogenic epitopes for immunotherapy
bull Develop personalized cancer vaccines
bull Monitor patient-specific anti-tumor antibody responses
bull Discover prognostic cancer neoepitopes
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Cancer Neoepitope Microarrays
PEPperCHIPreg Cancer Neoepitope Microarray Specifications
bull Microarray Content ndash cancer neoepitopes sequencing data custom peptide lists etc
bull Peptide Length ndash 15 amino acids by default (adjustable)
bull Peptide Numbers ndash up to 5500 different peptides printed in duplicate
bull Control Peptides ndash HA epitopes by default optional custom controls
bull Timeline ndash 4 weeks for microarray delivery 2 weeks for data analysis amp reporting
bull Microarray Example ndash 2750 wild type neoepitope peptide pairs printed in duplicate
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
bull unpurified amp purified custom peptides with varying QC levels (MS HPLC)
bull from 6 - 25 amino acids in 2-10 micromol scale
bull T-cell stimulation 8 - 15 amino acids each peptide with free amino and carboxyl termini
bull individual peptides and peptide pools optional modifications like acetylation phosphorylation etc
General Capacities
bull 8 peptide synthesizers 4 RP-HPLC units (analytical amp preparative)
bull more than 20 RP-HPLC columns
bull Bruker Ultraflex MALDI-TOF
Delivery Times
bull set of 384 peptides lt 2micromol scale (research grade) 6 weeks
bull set of 192 peptides 2-10 micromol scale (research grade) 4-5 weeks
bull for higher peptide purities (gt80 purity) delivery times may vary
rarr about 100 customers all over the world
Peptide Pools for T-Cell Stimulation
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
PEPperMAPreg Immune Monitoring
bull linear and conformational epitope mapping of immunogens with max epitope resolution
bull fingerprint analysis of B-cell responses at various time points before and after immunization
PEPperMAPreg HLA Class II Screening
bull linear mapping of HLA class II binding peptides for peptide vaccine design
bull identification of HLA II DR specific epitopes with stable recombinant HLA proteins
Peptide Pools for T-Cell Stimulation
bull unpurified amp purified custom peptides with varying QC levels from 6 - 25 amino acids
bull peptide pools with hundreds of peptides from 2-10 micromol scale in few weeks
Vaccine Development
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Infectious Diseases
Vaccine Development
Autoimmune Research
Antibody Analysis Peptide Target Binder
Cancer Research
PEPperCHIPreg Applications
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
PEPperMAPreg Protein Interaction Analysis
bull linear and conformational protein mapping service with max peptide resolution
bull including a custom peptide microarray with single or multiple target proteins
PEPperMAPreg Hit Discovery
bull target binder screening with ~35000 custom or random peptides (linear or cyclic)
bull initial peptide hit discovery for target proteins
PEPperMAPreg Peptide Display Hit Validation and Optimization
bull in-depth analysis of hit peptides of peptide display screening
bull peptide hit validation and high-throughput optimization by peptide substitution scans
Full Combinatorial Constrained Tripeptide Libraries
bull target binder screening with all possible cyclic constrained tripeptides
bull small-molecule-like peptide hit discovery for target proteins
Screening Options
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
For detailed presentations including case studies
please contact infopepperprintcom
PEPperCHIPreg Applications
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Customization
bull most flexible peptide microarray platform with any content
bull from up to 35000 different peptides per microarray to prototype test format
bull linear and cyclic constrained peptides
bull incorporation of peptides with posttranslational modifications
Quality
bull high peptide quality and chip reproducibility ISO 9001-certified quality
bull custom and adjusted glass slide coatings for best signal-to-noise ratios
Service
bull extensive knowhow from peptide selection to bioinformatics
bull custom and scientific reporting instead of machine-made reports
bull thorough scientific support and fast response times
Summary
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Antibody Characterization
bull Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 Mesalam AA Desombere I Farhoudi A Van Houtte F Verhoye L Ball J Dubuisson J Foung SKH Patel AH Persson MAA
Leroux-Roels G Meuleman P Virology 2018 514 30-41 doi 101016jvirol201710019 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam
AA Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J
Leroux-Roels G Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
bull Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein Gardner TJ Stein KR
Duty JA Schwarz TM Noriega VM Kraus T Moran TM Tortorella D Nat Commun 2016 7 13627 (Epub)
Autoimmune Research
bull Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype Fritzle MJ Hudson M Choi MY Mahler M Wang M Bentow C Milo J Baron M Autoimmunity Reviews 2018 doi 101016jautrev201801006 (Epub)
bull Identification of the Antigenic Epitopes of Maternal Autoantibodies in Autism Spectrum Disorders Edmiston E Jones KL Vu T Ashwood P
Van de Water J Brain Behavior and Immunity 2017 doi 101016jbbi201712014 (Epub)
bull Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays Weber LK Palermo A Kuumlgler J Armant O
Isse A Rentschler S Jaenisch T Hubbuch J Duumlbel S Nesterov-Mueller A Breitling F Loeffler FF J Immunol Meth 2017 443 45ndash54 (Epub)
bull Acquired Factor XIII inhibitor associated with mantle cell lymphoma Nixon CP Prsic EH Guertin CA Stevenson RL Sweeney JD
Transfusion 2016 doi 101111trf13947 (Epub)
bull Identification of novel antigens contributing to autoimmunity in cardiovascular diseases Muumlller AM Bockstahler M Hristov G Weiszlig C Fischer A
Korkmaz-Icoumlz S Giannitsis E Poller W Schultheiss HP Katus HA Kaya Z Clinical Immunology 2016 in press doi 101016jclim201609003 (Epub)
bull Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis Metz I Beiszligbarth T Ellenberger D Pache F
Stork L Ringelstein M Aktas O Jarius S Wildemann B Dihazi H Friede T Bruumlck W Ruprecht K
Paul F Neurol Neuroimmunol Neuroinflamm 2016 doi 101212NXI0000000000000204 (Epub)
Key Publications
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Cancer Research
bull Circulating GRP78 antibodies from ovarian cancer patients a promising tool for cancer cell targeting drug delivery system Van Hoesen K
Meynier S Ribaux PG Petignat P Delie Salmon F Cohen MB Oncotarget 2017 doi 1018632oncotarget22412 (Epub)
bull Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics Qendro V Lundgren DH
Palczewski S Hegde P Stevenson C Perpetua L Latifi A Merriman J Bugos G Han DK Proteomics 2017 doi 101002pmic201600318 (Epub)
bull Non-invasive glioblastoma immunoprofiling by printed peptide arrays Mock A Herold-Mende C OncoImmunology 2015
doi 1010802162402X20151069941 (Epub)
bull Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients Mock A Warta R Geisenberger C Bischoff R
Schulte A Lamszus K Stadler V Felgenhauer T Schichor C Schwartz C Matschke J Jungk C Ahmadi R Sahm F Capper D Glass R Tonn JC
Westphal M von Deimling A Unterberg A Bermejo JL Herold-Mende C Oncotarget 2015 (Epub)
Infectious Diseases and Vaccines
bull A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan J Sack BK
Oyen D Zenklusen I Piccoli L Barbieri S Foglierini M Silacci Fregni C Marcandalli J Jongo S Abdulla S Perez L Corradin G Varani L Sallusto F
Lee Sim BK Hoffman SL Kappe SHI Daubenberger C Wilson IA Lanzavecchia A Nature Medicine 2018 24401ndash407 doi 101038nm4513 (Epub)
bull Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution Palermo A Weber LK Rentschler S Isse A Sedlmayr M
Herbster K List V Hubbuch J Loeffler FF Nesterov-Mueller A Breitling F Biotechnol J 20171700197 doi 101002biot201700197 (Epub)
bull Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire MCLC Pol-Fachin L Coecirclho DF Viana IFT Magalhatildees T Cordeiro MT Fischer N Loeffler FF Jaenisch T Franca RF Marques ETA
Lins RD ACS Omega 2017 2(7) 3913-3920 doi 101021acsomega7b00608 (Epub)
bull Identification of protective B-cell epitopes within the novel malaria vaccine candidate P falciparum Schizont Egress Antigen-1 Nixon CE
Park S Pond-Tor S Raj D Lambert LE Orr-Gonzales S Barnafo EK Friedman JF Fried M Duffy PE Kurtis JD Clin Vaccine Immunol 2017
pii CVI00068-17 doi 101128CVI00068-17 (Epub)
bull A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo Desombere I Mesalam AA
Urbanowicz RA Van Houtte F Verhoye L Keck ZY Farhoudi A Vercauteren K Weening KE Baumert TF Patel AH Foung SKH Ball J Leroux-Roels G
Meuleman P Antiviral Research 2017 148 53-64 doi 101016jantiviral201710015 (Epub)
Key Publications
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Peptide Target Binder Development
bull Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis Ho M-H
Lamont RJ Xie HSci Rep 2017 7 1413 (Epub)
PEPperPRINT Technology
bull Combinatorial synthesis of peptide arrays with a laser printer Stadler V Felgenhauer T Beyer M Fernandez S Leibe K Guumlttler S Groumlning M
Koumlnig K Torralba G Hausmann M Lindenstruth V Nesterov A Block I Pipkorn R Poustka A Bischoff FR Breitling F Angew Chem Int Ed Engl
200847(37)7132-5 (PubMed)
bull Combinatorial synthesis of peptide arrays onto a microchip Beyer M Nesterov A Block I Koumlnig K Felgenhauer T Fernandez S Leibe K
Torralba G Hausmann M Trunk U Lindenstruth V Bischoff FR Stadler V Breitling F Science 2007 Dec 21318(5858)1888 (PubMed)
Key Publications
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
Application Notes
httpswwwpepperprintcomupdatesapplication-notes
Tutorial Video amp Webinar
httpswwwpepperprintcomupdateswebinars-and-movies
wwwpepperprintcom infopepperprintcom
wwwlinkedincomcompanypepperprint-gmbh
wwwtwittercompepperchip
wwwfacebookcompepperprintgmbh
More Information
![An integrated approach to epitope analysis II: A system ...€¦ · DeGroot reviews T-cell epitope mapping systems avail-able publically and developed commercially [31]. Many T-cell](https://img.pdfslide.us/doc/110x75/5f2a36467c09c723b54fd267/an-integrated-approach-to-epitope-analysis-ii-a-system-degroot-reviews-t-cell.jpg)
![Identification and fine mapping of a linear B cell epitope of human … · amino acid side chains of the epitope, the back-bone atoms or a combination of the two [3–5]. An antibody](https://img.pdfslide.us/doc/110x75/6070beb68dfde87d6b6cb470/identification-and-fine-mapping-of-a-linear-b-cell-epitope-of-human-amino-acid-side.jpg)
