Fluorescencia Glicina Buffer

Application: Tecan i-control Tecan i-control , 1.7.1.12Device: infinite 200Pro Serial number: 1011009185Firmware: V_3.14_03/10_InfiniTe (Apr 26 2010/11.18MAI, V_3.14_03/10_InfiniTe (Apr 26 2010/11.18.37)

Date: 25/05/2011Time: 13:02:31

System PC-IBEC01User PC-IBEC01\Marina GiannottiPlate Corning 96 Flat Bottom Black Polystyrol [COS96fb.pdfx]Plate-ID (Stacker)

Label: Label2Mode Fluorescence Top ReadingEmission Wavelength Start 280 nmEmission Wavelength End 850 nmEmission Wavelength Step Size 2 nmEmission Scan Number 286Excitation Wavelength 365 nmBandwidth (Em) 280...850: 20 nmBandwidth (Ex) (Range 1) 230...315: 5 nmBandwidth (Ex) (Range 2) 316...850: 10 nmGain 100 ManualNumber of Flashes 25Integration Time 20 µsLag Time 0 µsSettle Time 0 msZ-Position (Manual) 20000 µmPart of Plate H1-H1Start Time: 25/05/2011 13:02:31

Temperature: 26.3 °CWavel. 280 282 284 286 288 290 292H1 43 25 57 35 30 49 43

End Time: 25/05/2011 13:05:45

200 250 300 350 400 450 500 550 6000

500

1000

1500

2000

2500

3000

3500

4000

4500

5000

43 25 57 35 30 49 43 75 9 39 24 24 45 25 21 23 17 21 24 22 17 12 21 13 13 18 17 63 829 4556 54772 OVER

OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER

OVER OVER OVER OVER OVER OVER OVER

43 25 57 35 30 49 43 75 9 39 24 24 45 25 21 23 17 21 24 22 17 12 21 13 13 18 17 63 829 4556 54772 OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER

E1

Tecan.At.Common, 1.7.1.12 Tecan.At.Common.Controls, 1.7.1.12 Tecan.At.Common.Dialogs, 1.7.1.12 Tecan.At.Common.MathLib, 1.7.1.12 Tecan.At.Common.MCS, 1.7.1.12 Tecan.At.Common.Results, 1.7.1.12 Tecan.At.Communication.Common, 1.7.1.12 Tecan.At.Communication.Port.IP, 1.7.1.12 Tecan.At.Communication.Port.RS232, 1.7.1.12 Tecan.At.Communication.Port.SIM.Common, 1.7.1.12 Tecan.At.Communication.Port.USB, 1.7.1.12 Tecan.At.Communication.Server, 1.7.1.12 Tecan.At.Communication.SIM.AMR, 1.7.1.12 Tecan.At.Communication.SIM.AMRPlus, 1.7.1.12 Tecan.At.Communication.SIM.Connect, 1.7.1.12 Tecan.At.Communication.SIM.GeniosUltra, 1.7.1.12 Tecan.At.Communication.SIM.Safire3, 1.7.1.12 Tecan.At.Communication.SIM.SunriseMini, 1.7.1.12 Tecan.At.DocumentManagement, 1.7.1.12 Tecan.At.DocumentManagement.Reader, 1.7.1.12 Tecan.At.FileManagement, 1.7.1.12 Tecan.At.Instrument.Common, 1.7.1.12 Tecan.At.Instrument.Common.Reader, 1.7.1.12 Tecan.At.Instrument.Common.Stacker, 1.7.1.12 Tecan.At.Instrument.Reader.AMR, 1.7.1.12 Tecan.At.Instrument.Reader.AMRPlus, 1.7.1.12 Tecan.At.Instrument.Reader.GeniosUltra, 1.7.1.12 Tecan.At.Instrument.Reader.Safire3, 1.7.1.12 Tecan.At.Instrument.Reader.SunriseMini, 1.7.1.12 Tecan.At.Instrument.Server, 1.7.1.12 Tecan.At.Instrument.Stacker.Connect, 1.7.1.12 Tecan.At.Measurement.BuiltInTest.Common, 1.7.1.12 Tecan.At.Measurement.Common, 1.7.1.12 Tecan.At.Measurement.Server, 1.7.1.12 Tecan.At.Stacker.Server, 1.7.1.12 Tecan.At.Win32, 1.7.1.12 Tecan.At.XFluor, 1.7.1.12 Tecan.At.XFluor.Connect.Reader, 1.7.1.12 Tecan.At.XFluor.Core, 1.7.1.12 Tecan.At.XFluor.Device, 1.7.1.12 Tecan.At.XFluor.Device.AMR, 1.7.1.12 Tecan.At.XFluor.Device.AMRPlus, 1.7.1.12 Tecan.At.XFluor.Device.GeniosUltra, 1.7.1.12 Tecan.At.XFluor.Device.Reader, 1.7.1.12 Tecan.At.XFluor.Device.Safire3, 1.7.1.12 Tecan.At.XFluor.Device.SunriseMini, 1.7.1.12 Tecan.At.XFluor.Editor, 1.7.1.12 Tecan.At.XFluor.ExcelOutput, 1.7.1.12 Tecan.At.XFluor.ReaderEditor, 1.7.1.12 Tecan.At.XmlSettings, 1.7.1.12

E3

EHC, V_3.14_03/10_InfiniTe (Apr 26 2010/11.18.37) MTP, V_3.14_03/10_InfiniTe (Apr 26 2010/11.18.37) CUV, V_3.14_03/10_InfiniTe (Apr 26 2010/11.18.37) HCP, V_2.02_05/06_HCP (May 23 2006/14.05.27) LUM, V_2.00_04/06_LUMINESCENCE (Apr 5 2006/08.57.29) MEM, V_2.12_03/08_MCR (Apr 3 2008/16.03.31) MEX, V_2.13_04/10_MCR (Apr 25 2010/17.39.16) ZSCAN, V_3.14_03/10_InfiniTe (Apr 26 2010/11.18.37)

Serial number of connected stacker:

Corning 96 Flat Bottom Black Polystyrol [COS96fb.pdfx]

294 296 298 300 302 304 306 30875 9 39 24 24 45 25 21

200 250 300 350 400 450 500 550 6000

500

1000

1500

2000

2500

3000

3500

4000

4500

5000

43 25 57 35 30 49 43 75 9 39 24 24 45 25 21 23 17 21 24 22 17 12 21 13 13 18 17 63 829 4556 54772 OVER

OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER

OVER OVER OVER OVER OVER OVER OVER

43 25 57 35 30 49 43 75 9 39 24 24 45 25 21 23 17 21 24 22 17 12 21 13 13 18 17 63 829 4556 54772 OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER OVER

310 312 314 316 318 320 322 32423 17 21 24 22 17 12 21

326 328 330 332 334 336 338 34013 13 18 17 63 829 4556 54772

342 344 346 348 350 352 354 356OVER OVER OVER OVER OVER OVER OVER OVER



390 392 394 396 398 400 402 40470703 4027 358 145 109 118 150 164

406 408 410 412 414 416 418 420178 197 217 226 226 226 220 210

422 424 426 428 430 432 434 436189 176 162 151 133 109 99 92

438 440 442 444 446 448 450 45287 80 79 75 72 72 69 65

454 456 458 460 462 464 466 46864 64 57 54 53 49 48 46

470 472 474 476 478 480 482 48445 43 40 40 39 38 35 34

486 488 490 492 494 496 498 50032 31 28 29 28 26 24 23

502 504 506 508 510 512 514 51621 19 19 18 19 17 17 14

518 520 522 524 526 528 530 53214 15 13 13 13 12 12 11

534 536 538 540 542 544 546 54811 11 9 8 10 9 7 8

550 552 554 556 558 560 562 5647 6 6 7 6 7 5 6

566 568 570 572 574 576 578 5805 4 5 5 6 4 6 4

582 584 586 588 590 592 594 5965 3 5 4 4 3 7 5

598 600 602 604 606 608 610 6126 4 3 4 5 6 4 4

614 616 618 620 622 624 626 6284 4 4 4 5 3 3 5

630 632 634 636 638 640 642 6444 3 5 4 4 5 5 4

646 648 650 652 654 656 658 6603 4 7 5 5 4 6 4

662 664 666 668 670 672 674 6763 6 3 6 6 4 4 5

678 680 682 684 686 688 690 6924 7 4 5 5 6 3 6

694 696 698 700 702 704 706 70810 5 3 4 4 5 5 4

710 712 714 716 718 720 722 7247 4 5 7 5 6 6 6

726 728 730 732 734 736 738 7406 7 4 8 6 4 6 4

742 744 746 748 750 752 754 7564 6 4 4 4 4 3 5

758 760 762 764 766 768 770 7724 4 4 5 3 4 5 5

774 776 778 780 782 784 786 7887 3 5 5 4 7 6 6

790 792 794 796 798 800 802 8044 7 5 6 4 5 7 7

806 808 810 812 814 816 818 8205 8 4 3 8 6 6 5

822 824 826 828 830 832 834 8367 7 10 9 8 6 8 6

838 840 842 844 846 848 85011 11 6 15 12 14 21


Date: 25/05/2011Time: 12:46:25


Label: Label2Mode Fluorescence Top ReadingEmission Wavelength Start 280 nmEmission Wavelength End 850 nmEmission Wavelength Step Size 2 nmEmission Scan Number 286Excitation Wavelength 230 nmBandwidth (Em) 280...850: 20 nmBandwidth (Ex) (Range 1) 230...315: 5 nmBandwidth (Ex) (Range 2) 316...850: 10 nmGain 100 ManualNumber of Flashes 25Integration Time 20 µsLag Time 0 µsSettle Time 0 msZ-Position (Manual) 20000 µmPart of Plate H1-H2Start Time: 25/05/2011 12:46:25

Temperature: 24.1 °CWavel. 280 282 284 286 288 290 292H1 3018 3138 3953 4439 5163 5919 6380H2 7020 7107 8076 10227 11076 12589 13820

End Time: 25/05/2011 12:52:47

E1


E3




294 296 298 300 302 304 306 3087235 8134 8871 9568 9585 10681 11462 11710

15331 17707 19151 20310 20520 23042 24521 25106

310 312 314 316 318 320 322 32411824 12478 12708 12942 13063 12991 12967 1273025493 26998 27393 27919 28131 28206 28080 27469

326 328 330 332 334 336 338 34012452 12122 11531 11119 10530 9949 9297 888627222 26247 25223 24227 23024 21529 20362 19269

342 344 346 348 350 352 354 3568119 7497 6807 6314 5658 5085 4567 4126

17698 16228 14845 13632 12238 11020 9864 8902

358 360 362 364 366 368 370 3723637 3412 2905 2616 2218 1985 1765 15387827 7351 6304 5659 4778 4281 3762 3302

374 376 378 380 382 384 386 3881371 1228 1086 963 832 725 633 5642924 2618 2317 2031 1707 1548 1340 1199

390 392 394 396 398 400 402 404502 432 364 318 272 246 196 171

1054 907 760 654 556 503 394 342

406 408 410 412 414 416 418 420154 143 126 114 101 90 87 76309 284 243 212 189 167 148 142

422 424 426 428 430 432 434 43671 61 60 54 51 49 45 44

120 112 106 98 87 80 73 67

438 440 442 444 446 448 450 45241 45 50 70 82 118 137 15065 64 74 90 103 140 161 176

454 456 458 460 462 464 466 468176 186 195 200 187 172 158 129202 211 224 220 212 193 176 146

470 472 474 476 478 480 482 484104 87 67 51 34 21 16 12118 97 75 59 41 27 22 19

486 488 490 492 494 496 498 50014 11 11 11 10 11 9 1018 16 16 16 15 15 13 13

502 504 506 508 510 512 514 5168 8 7 8 7 7 8 7

13 12 11 11 10 10 9 9

518 520 522 524 526 528 530 5328 7 6 5 5 6 6 69 9 7 9 8 9 7 8

534 536 538 540 542 544 546 5485 6 6 5 6 5 4 58 6 7 6 7 5 6 5

550 552 554 556 558 560 562 5645 5 4 3 4 4 2 47 5 5 7 5 6 4 5

566 568 570 572 574 576 578 5803 3 2 3 3 4 3 34 7 4 5 5 6 4 5

582 584 586 588 590 592 594 5963 3 2 2 3 3 1 24 5 4 5 5 4 5 5

598 600 602 604 606 608 610 6122 2 3 3 2 1 2 35 5 3 5 4 5 6 3

614 616 618 620 622 624 626 6283 2 2 2 4 4 1 35 5 6 4 4 4 5 4

630 632 634 636 638 640 642 6442 2 3 2 3 3 3 25 6 4 4 5 7 6 6

646 648 650 652 654 656 658 6603 2 3 4 4 3 5 34 6 6 7 5 6 4 6

662 664 666 668 670 672 674 6764 1 3 4 2 3 2 48 5 6 8 7 5 7 6

678 680 682 684 686 688 690 6924 5 4 7 7 4 7 56 6 8 6 8 9 7 7

694 696 698 700 702 704 706 7085 3 6 7 4 4 4 3

10 8 7 6 6 5 7 7

710 712 714 716 718 720 722 7244 3 2 1 3 3 3 36 6 7 6 6 5 5 5

726 728 730 732 734 736 738 7404 2 2 3 3 2 1 36 6 6 6 4 6 5 3

742 744 746 748 750 752 754 7564 1 5 2 3 4 2 25 5 3 6 5 6 5 7

758 760 762 764 766 768 770 7721 3 3 4 5 4 4 37 6 5 5 6 7 5 5

774 776 778 780 782 784 786 7883 4 3 2 3 5 3 35 4 4 7 6 7 4 5

790 792 794 796 798 800 802 8046 4 5 3 4 4 3 47 7 5 9 6 9 8 2

806 808 810 812 814 816 818 8205 5 9 5 4 9 5 76 7 7 9 8 9 10 8

200 250 300 350 400 450 500 550 6000

500

1000

1500

2000

2500

3000

3500

4000

4500

5000

Row 36Row 37

200 250 300 350 400 450 500 550 6000

500

1000

1500

2000

2500

3000

3500

4000

4500

5000

Row 36Row 37

822 824 826 828 830 832 834 8365 5 5 8 11 6 9 49 9 8 10 12 12 12 16

1 12 23 34 45 56 67 78 89 1001111221331441551661771881992102212322432542652760

5000

10000

15000

20000

25000

30000

Row 35Row 36Row 37

200 250 300 350 400 450 500 550 6000

500

1000

1500

2000

2500

3000

3500

4000

4500

5000

Row 36Row 37

1 12 23 34 45 56 67 78 89 1001111221331441551661771881992102212322432542652760

5000

10000

15000

20000

25000

30000

Row 35Row 36Row 37

200 250 300 350 400 450 500 550 6000

500

1000

1500

2000

2500

3000

3500

4000

4500

5000

Row 36Row 37

838 840 842 844 846 848 8509 8 11 12 11 14 11

19 17 20 20 27 19 34

1 12 23 34 45 56 67 78 89 1001111221331441551661771881992102212322432542652760

5000

10000

15000

20000

25000

30000

Row 35Row 36Row 37

200 250 300 350 400 450 500 550 6000

500

1000

1500

2000

2500

3000

3500

4000

4500

5000

Row 36Row 37

1 12 23 34 45 56 67 78 89 1001111221331441551661771881992102212322432542652760

5000

10000

15000

20000

25000

30000

Row 35Row 36Row 37

200 250 300 350 400 450 500 550 6000

500

1000

1500

2000

2500

3000

3500

4000

4500

5000

Row 36Row 37


Date: 25/05/2011Time: 12:42:00


Label: Label1Mode Fluorescence Top ReadingExcitation Wavelength 365 nmEmission Wavelength 450 nmExcitation Bandwidth 9 nmEmission Bandwidth 20 nmGain 238 Optimal (100%)Number of Flashes 25Integration Time 20 µsLag Time 0 µsSettle Time 0 msZ-Position (Calculated From: H1) 20439 µmPart of Plate H1-H2Start Time: 25/05/2011 12:43:58

Temperature: 24 °C<> 1 2H 46298 35489

End Time: 25/05/2011 12:44:03

E1


E3





Date: 25/05/2011Time: 12:40:05


Fluorescence

E1

Tecan.At.Common, 1.7.1.12 Tecan.At.Common.Controls, 1.7.1.12 Tecan.At.Common.Dialogs, 1.7.1.12 Tecan.At.Common.MCS, 1.7.1.12 Tecan.At.Common.Results, 1.7.1.12 Tecan.At.Communication.Common, 1.7.1.12 Tecan.At.Communication.Port.IP, 1.7.1.12 Tecan.At.Communication.Port.RS232, 1.7.1.12 Tecan.At.Communication.Port.SIM.Common, 1.7.1.12 Tecan.At.Communication.Port.USB, 1.7.1.12 Tecan.At.Communication.Server, 1.7.1.12 Tecan.At.Communication.SIM.AMR, 1.7.1.12 Tecan.At.Communication.SIM.AMRPlus, 1.7.1.12 Tecan.At.Communication.SIM.Connect, 1.7.1.12 Tecan.At.Communication.SIM.GeniosUltra, 1.7.1.12 Tecan.At.Communication.SIM.Safire3, 1.7.1.12 Tecan.At.Communication.SIM.SunriseMini, 1.7.1.12 Tecan.At.DocumentManagement, 1.7.1.12 Tecan.At.DocumentManagement.Reader, 1.7.1.12 Tecan.At.FileManagement, 1.7.1.12 Tecan.At.Instrument.Common, 1.7.1.12 Tecan.At.Instrument.Common.Reader, 1.7.1.12 Tecan.At.Instrument.Common.Stacker, 1.7.1.12 Tecan.At.Instrument.Reader.AMR, 1.7.1.12 Tecan.At.Instrument.Reader.AMRPlus, 1.7.1.12 Tecan.At.Instrument.Reader.GeniosUltra, 1.7.1.12 Tecan.At.Instrument.Reader.Safire3, 1.7.1.12 Tecan.At.Instrument.Reader.SunriseMini, 1.7.1.12 Tecan.At.Instrument.Server, 1.7.1.12 Tecan.At.Instrument.Stacker.Connect, 1.7.1.12 Tecan.At.Measurement.BuiltInTest.Common, 1.7.1.12 Tecan.At.Measurement.Common, 1.7.1.12 Tecan.At.Measurement.Server, 1.7.1.12 Tecan.At.Stacker.Server, 1.7.1.12 Tecan.At.Win32, 1.7.1.12 Tecan.At.XFluor, 1.7.1.12 Tecan.At.XFluor.Connect.Reader, 1.7.1.12 Tecan.At.XFluor.Core, 1.7.1.12 Tecan.At.XFluor.Device, 1.7.1.12 Tecan.At.XFluor.Device.AMR, 1.7.1.12 Tecan.At.XFluor.Device.AMRPlus, 1.7.1.12 Tecan.At.XFluor.Device.GeniosUltra, 1.7.1.12 Tecan.At.XFluor.Device.Reader, 1.7.1.12 Tecan.At.XFluor.Device.Safire3, 1.7.1.12 Tecan.At.XFluor.Device.SunriseMini, 1.7.1.12 Tecan.At.XFluor.Editor, 1.7.1.12 Tecan.At.XFluor.ExcelOutput, 1.7.1.12 Tecan.At.XFluor.ReaderEditor, 1.7.1.12 Tecan.At.XmlSettings, 1.7.1.12

E3




Documents

Fluorescencia Glicina Buffer