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Fluid Mechanics of the eye:
recent progress at the University of Genoa.
Jan O. Pralits
Department of Civil, Chemical and Environmental EngineeringUniversity of Genoa, Italy
June 17, 2015
The work presented has been carried out by:
Rodolfo Repetto DICCA, University of Genoa, Italy;
Jennifer Siggers Imperial College London, UK;
Jan O. Pralits DICCA, University of Genoa, Italy;
Krystyna Isakova DICCA, University of Genoa, Italy;
Peyman Davvalo Khongar DICCA, University of Genoa, Italy;
Damiano Natali DICCA, University of Genoa, Italy;
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 1 / 63
1 Introduction
2 Stability of the interface after vitreoretinal surgery
3 Flow of aqueous humour with an intraocular lens
4 Retinal break
5 Equilibrium shape of the aqueous humour-vitreous substitute interface
6 References
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 2 / 63
Introduction
Anatomy of the eye
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 3 / 63
Introduction
Anterior chamber I
Flow mechanisms
Flow induced by aqueous production/drainage:Aqueous humor is produced by the ciliary body, and then flows throughthe posterior chamber, the pupil and the anterior chamber, from whereit is drained into the trabecular meshwork. (3 µl/min)
Flow induced during myosis/mydriasis:During pupil contraction (myosis), a flow from the posterior to theanterior chamber of the eye is generated, which is intense, although itonly lasts a short time, typically less than 1 s. (middle figure)
Buoyancy-driven flow:It is well known that, since the posterior surface of the cornea istypically cooler than the iris and lens. We prescribed a temperature of34 C on the cornea and 37 C on all other surfaces.
Flow induced by saccades of the eye:We consider the flow generated in the anterior chamber by rotations ofthe eye bulb by modeling isolated rotations using the analyticalrelationship proposed by Repetto et al. (2005) which provides theangular velocity of the eye as a function of time. (bottom figure)
0
5e-10
1e-09
1.5e-09
2e-09
2.5e-09
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8
Inle
t flux [m
3/s
]
Time [s]
(a)
0
50
100
150
200
250
300
350
0 0.01 0.02 0.03 0.04 0.05 0.06
Angula
r velo
city [deg/s
]
Time [s]
(b)A= 10 deg
20 deg
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 4 / 63
Introduction
Vitreous characteristics and functions
Vitreous composition
The main constituents are
Water (99%);
hyaluronic acid (HA);
collagen fibrils.
Its structure consists of long, thick, non-branching collagenfibrils suspended in hyaluronic acid.
Normal vitreous characteristicsThe healthy vitreous in youth is a gel-like material with visco-elastic mechanical properties,which have been measured by several authors (Lee et al., 1992; Nickerson et al., 2008;Swindle et al., 2008).
In the outermost part of the vitreous, named vitreous cortex, the concentration of collagenfibrils and HA is higher.
The vitreous cortex is in contact with the Internal Limiting Membrane (ILM) of the retina.
Physiological roles of the vitreousSupport function for the retina and filling-up function for the vitreous body cavity;
diffusion barrier between the anterior and posterior segment of the eye;
establishment of an unhindered path of light.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 5 / 63
Introduction
Vitreous ageing
With advancing age the vitreous typically undergoes significant changes in structure.
Disintegration of the gel structure which leads to vitreousliquefaction (synchisys). This leads to an approximatelylinear increase in the volume of liquid vitreous with time.Liquefaction can be as much extended as to interest thewhole vitreous chamber.
Shrinking of the vitreous gel (syneresis) leading to thedetachment of the gel vitreous from the retina in certainregions of the vitreous chamber. This process typically occursin the posterior segment of the eye and is called posteriorvitreous detachment (PVD). It is a pathophysiologiccondition of the vitreous.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 6 / 63
Introduction
Retinal detachment
Posterior vitreous detachment (PVD) andvitreous degeneration:
more common in myopic eyes;
preceded by changes in vitreousmacromolecular structure and invitreoretinal interface → possiblymechanical reasons.
If the retina detaches from the underlyinglayers → loss of vision;
Rhegmatogeneous retinal detachment:
fluid enters through a retinal break into thesub retinal space and peels off the retina.
Risk factors:
myopia;
posterior vitreous detachment (PVD);
lattice degeneration;
...
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 7 / 63
Introduction
Scleral buckling and vitrectomy
Scleral bluckling
Scleral buckling is the application of a rubberband around the eyeball at the site of a retinaltear in order to promote reachtachment of theretina.
Vitrectomy
The vitreous may be completely replaced withtamponade fluids: silicon oils, water, gas, ...,usually immiscible with the eye’s own aqueoushumor
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 8 / 63
Introduction
Investigations
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 9 / 63
Stability of the interface after vitreoretinal surgery
A model for the linear stability of the interface between aqueoushumor and vitreous substitutes after vitreoretinal surgery
K. Isakova1, J. O. Pralits1, R. Repetto1, M. R. Romano2
1 Department of Civil, Chemical and Environmental Engineering, University of Genoa, Italy,2 Istituto Clinico Humanitas, Milano, Italy
Published: Physics of Fluids 2014; 26:124101
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 10 / 63
Stability of the interface after vitreoretinal surgery
Stability of the interface between aqueous humor and vitreoussubstitutes after vitreoretinal surgery
Retinal detachment
Warning signs of retinal detachment:
Flashing lights.
Sudden appearance of floaters.
Shadows on the periphery of your vision.
Gray curtain across your field of vision.
Vitrectomy
The vitreous may be completely replaced withtamponade fluids: silicon oils, air, gas, ...
Denoted tamponade liquids
Purpose: Induce an instantaneousinterruption of an open communicationbetween the subretinal space/retinalpigment epithelial cells and the pre-retinalspace.
Healing: a scar should form as the cellsabsorb the remaining liquid.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 11 / 63
Stability of the interface after vitreoretinal surgery
Fluids commonly used as a vitreous substitutes
Silicone oils;960 ≤ ρ∗ ≤ 1290 kg/m3
10−4 ≤ ν∗ ≤ 5× 10−3 m/s2
σ∗ ≈ 0.05 N/m
Perfluorocarbon liquids;1760 ≤ ρ∗ ≤ 2030 kg/m3
8× 10−7 ≤ ν∗ ≤ 8× 10−6 m/s2
σ∗ ≈ 0.05 N/m
Semifluorinated alkane liquids;1350 ≤ ρ∗ ≤ 1620 kg/m3
4.6× 10 ≤ ν∗ ≤ 10−3 m/s2
0.035 ≤ σ∗ ≤ 0.05 N/m
The choice of tamponade liquid depends on thespecific case
The tabulated fluids are immiscible withwater and commonly used in surgery
A lighter fluid (cf. water) is used totamponade in the upper part
A heavier fluid is used to tamponade in thelower part
High surface tension is preferred to a lowvalue (EXPERIENCE)
High value of viscosity (cf. water) ispreferred to a low value (EXPERIENCE)
What could happen otherwise ?
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 12 / 63
Stability of the interface after vitreoretinal surgery
Emulsification
Emulsification leads to loss of vision, not satisfactory
Figure: Emulsification of vitreous substitutes in the vitreous chamber
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 13 / 63
Stability of the interface after vitreoretinal surgery
Summary & Motivation
SummaryFrom experience it is known that tamponade fluids with high surface tension and highviscosity (compared to water) are less prone to emulsify
It is also know that initially ”good” tamponade fluids tend to change with time, for instancea decrease of surface tension due to surfactants, which leads to emulsification.
It is generally believed that shear stresses at the tamponade fluid-aqueous interfacegenerated during eye rotations play a crucial role in the generation of an emulsion.
The tamponade liquid needs to stay for a period of months so it is of interest to know howemulsification can be avoided.
Our analysisWe want to understand how emulsification, or the initial stages leading to emulsification, arerelated to the parameters (surface tension, viscosity, density, real conditions).
As a first study we focus on the stability characteristics of the interface in order to see if ithas any role.
A linear stability analysis, of wave like solutions, is used.
The evolution of the disturbance kinetic energy is analyzed.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 14 / 63
Stability of the interface after vitreoretinal surgery
Mathematical model I
The geometry
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 15 / 63
Stability of the interface after vitreoretinal surgery
Mathematical model II
Underlying assumptions
Figure: Geometry of the problem
d∗ << R∗
2D-model;
flat wall oscillating harmonically;
semi-infinite domain;
small perturbations;
quasi-steady approach.
Stokes problem when 1 = 2
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 16 / 63
Stability of the interface after vitreoretinal surgery
Scaling and Dimensionless Parameters
x =x∗
d∗, ui =
u∗iV ∗0
, pi =p∗i
ρ∗1 V ∗20
, t =V ∗0d∗
t, ω =d∗
V ∗0ω∗
m =µ∗2µ∗1
γ =ρ∗2ρ∗1
Re =V ∗0 d∗
ν∗1Fr =
V ∗0√g∗d∗
S =σ∗
ρ∗1 d∗V ∗20
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 17 / 63
Stability of the interface after vitreoretinal surgery
Basic flow
Analytical solution
Parallel time-dependent flow
U1(y , t) = (c1e−ay + c2eey )e iωt + c.c.,
U2(y , t) = c3e−by e iωt + c.c.,
∂P1
∂y= −Fr−2,
∂P2
∂y= −γFr−2,
where
a =√
iωR, b =
√iγωR
m.
and c1, c2, c3 are functions of a, b,m.
0
10
20
30
40
50
60
70
80
-1 -0.5 0 0.5 1
y
U
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 18 / 63
Stability of the interface after vitreoretinal surgery
Linear stability analysis
Flow decomposition:ui = Ui + ui
′, vi = vi′ pi = Pi + p′i
Boundary conditions:
u′1(0, t) = v ′1(0, t) = 0 and u′2(y , t)→ 0, v ′2(y , t)→ 0 as y →∞
Interface: (y∗ = d∗) introducing also the perturbation of the interface η′
Continuity of the perturbation velocity components across the interface
Continuity of the tangential stress of across the interface
The wall normal stress is balanced by the surface tension
A quasi-steady approach is assumed with two-dimensional wave-like solutions as:
ξi = e iα(x−Ωt)ξi (y , τ) + c.c
where0 ≤ τ ≤ 2π/ω
The system of equations is reduced introducing the perturbation stream function giving twoOrr-Sommerfeld equations, discretized using finite differences, solved using an inverse iterationalgorithm.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 19 / 63
Stability of the interface after vitreoretinal surgery
Range of variability of the dimensionless parameters
0.1
1
10
0 0.02 0.04 0.06
Re
ω
(a)
0.1
1
10
10 100 1000
Re
S
(b)
0.1
1
10
0 5 10 15 20 25
Re
F r
(c)
Figure: Relationship between Re − ω, Re − S and Re − Fr obtained adopting feasible values of eye movement.From thin to thick curves: d = 1× 10−5m, d = 3× 10−5m, d = 1× 10−4m
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 20 / 63
Stability of the interface after vitreoretinal surgery
Neutral Curves
160
180
200
220
240
0.3 0.35 0.4 0.45 0.5
L=
2π/α
ωt/π
Unstable
Stable
m = 510
15
20
Figure: S = 14, γ = 1.0, Re = 7, ω = 0.001
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 21 / 63
Stability of the interface after vitreoretinal surgery
Energy analysis
The disturbance kinetic energy is given by 3 contributions: production, dissipation and interfacerelated terms
α
2π
dE
dt= −
∫ 1
0
u1v1U′1dy − γ∫ +∞
1
u2v2U′2dy
−1
Re
∫ 1
0
[(∂u1
∂x
)2+(∂u1
∂y
)2+(∂v1
∂x
)2+(∂v1
∂y
)2]dy
−m
Re
∫ +∞
1
[(∂u2
∂x
)2+(∂u2
∂y
)2+(∂v2
∂x
)2+(∂v2
∂y
)2]dy
(v1
[(γ − 1)Fr−2 + α
2S]η −
v1
Re(∂v1
∂y− m
∂v2
∂y) +
1
Re(u1
∂u1
∂y− mu2
∂u2
∂y))∣∣∣
y=1. (4)
The different contributions of (4) are commonly presented in terms of growth rates. The sum can directly becompared with the solution of the eigenvalue problem governing the linear stability problem.
1
2αE
dE
dt= Im(Ω).
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 22 / 63
Stability of the interface after vitreoretinal surgery
Results from the energy analysis
-1
-0.5
0
0.5
1
160 180 200 220 240
L = 2π/α
(a)
stable unstable
-1
-0.5
0
0.5
1
0.3 0.35 0.4 0.45 0.5
ωt/π
(b)
unstable stablestable
Figure: The different contributions from the energy analysis as a function of (a) L for ωt/π = 0.4 (a) and (b)ωt/π for L = 200. In both figures S = 14, γ = 1.0, R = 7, m = 5, ω = 0.001. Lines: thin solid (production),dashed (dissipation), dotted (interface), thick solid (growth rate scaled).
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 23 / 63
Stability of the interface after vitreoretinal surgery
Shortes unstable wave length
The shortest unstable wave length as a function of S, Re and γ.
20
40
60
80
100
120
140
160
180
200
0 2 4 6 8 10 12 14
Lm
in
S
(a)
Unstable
Stable
20
40
60
80
100
120
140
160
180
200
0 20 40 60
Lm
in
Re
(b)
Unstable
Stable
20
40
60
80
100
120
140
160
180
200
0.5 1 1.5 2
Lm
in
γ
(c)
Unstable
Stable
Figure: Length of the shortest unstable perturbation Lmin versus S (a), Re (b), and γ (c) with ω = 0.001 andm = 5. The values of Re = 7 in (a) and (c), S = 14 in (b) and (c), and γ = 1 in (a) and (b), respectively.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 24 / 63
Stability of the interface after vitreoretinal surgery
Verification of the quasi-steady approach
In order for the quasi steady approach to be valid the perturbation frequency should be largerthan the base flow frequency (scale separation).
160
170
180
190
200
210
220
230
240
250
0.3 0.35 0.4 0.45 0.5
L=
2π/α
ωt/π
10
15
20
5
Figure: Neutral stability curve (solid line) in the (ωt/π)− L plane for the case m = 5, Re = 7 , ω = 0.001,S = 14, γ = 1. The dotted lines show the values of the ratio α<(Ω)/ω.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 25 / 63
Stability of the interface after vitreoretinal surgery
Conclusions and Continuation
Monitoring the shortest unstable wave length (critical wave length) we have seen that:
Increasing the viscosity, ratio the critical wave length increases (stabilizing for the Eye)
Increasing the surface tension, the critical wave length increases (stabilizing for the Eye)
Increasing the Reynolds no., the critical wave length decreases (destabilizing for the Eye)
Increasing the density ratio, the critical wave length decreases (destabilizing for the Eye)
The first two is ”in line” with realistic observations.
For realistic values of R,S , γ,m, ω, d∗ the critical wave length ≈ 5 mm, which is about halfthe Eye radius.
However, the growth rate is instantaneous and the waves unstable only during certainintervals of one period. (cf. turbulent burst in the classical Stokes II problem). No sustainedgrowth over one period is guaranteed.
This analysis is far from explaining the onset of emulsion but a first step to rule out (or not)different physical mechanisms.
Next step...
Floquet analysis
Non-modal analysis
Include curvature, ...., more realistic geometry (circle, sphere)
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 26 / 63
Flow of aqueous humour with an intraocular lens
Phakic iris-fixated intraocular lens placement in the anteriorchamber: effects on aqueous flow
R. Repetto1, J. O. Pralits1, J. H. Siggers2 and P. Soleri3
1 Department of Civil, Chemical and Environmental Engineering, University of Genoa, Italy,2 Department of Bioengineering, Imperial College London, London SW7 2AZ, UK,
3 Ophtec BV, Schweitzerlaan 15, 9728 NR, Groningen, The Netherlands
Published: investigative ophtamology & visual science (iovs) 2015; 56(5):3061-3068.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 27 / 63
Flow of aqueous humour with an intraocular lens
Flow of aqueous humour with an intraocular lens
Phakic intraocular lenses
MotivationIntraocular lenses decrease endothelial cell density in some patients. Possible reasons include:
Excess shear stress due to altered flow of aqueous
Impaired transport of nutrients due to altered flow
Non-ideal placement of lens, with respect to iris and other tissues
Impact of external body on eye (e.g. accidental impact, patient touching eyes)
This study: Investigate the first possibility, by comparing the shear stress both in the presenceof and without the lens. Moreover, monitor the intra-ocular pressure (IOP).
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 28 / 63
Flow of aqueous humour with an intraocular lens
Description of the model
Governing equations:
∇ · u =0
ρ
(∂u
∂t+ u ·∇u
)=−∇p +∇2u + ρg
ρcp
(∂T
∂t+ u ·∇T
)=ka∇2T
Assumptions:
Boussinesq approximation (ρ is constant,except in the gravitational acceleration termin which ρ = ρ0(1− α(T − T0)))
Numerical solution:
All solutions are obtained with OpenFOAM
Solvers were first tested on cases whereanalytical solutions exist.
Definitions:
Symbol Definitionu velocityp pressureρ density of aqueousµ viscosity of aqueousg acceleration due to gravitycp specific heat at constant pressureT temperatureka thermal conductivity of aqueous
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 29 / 63
Flow of aqueous humour with an intraocular lens
Model geometry
Figure: (left) ”real” anterior chamber, (right) cross-section of the idealized anterior chamber used in thesimulations.
Figure: (a) Geometry of the pIOL consisting of a lens and two haptics that have claws that allow the lens to beattached to the iris, (b) pIOL placed in the anterior chamber.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 30 / 63
Flow of aqueous humour with an intraocular lens
Flow mechanisms
Flow induced by aqueous production/drainage:Aqueous humor is produced by the ciliary body, and then flows throughthe posterior chamber, the pupil and the anterior chamber, from whereit is drained into the trabecular meshwork. (3 µl/min)
Flow induced during miosis:During pupil contraction (miosis), a flow from the posterior to theanterior chamber of the eye is generated, which is intense, although itonly lasts a short time, typically less than 1 s. (middle figure)
Buoyancy-driven flow:It is well known that, since the posterior surface of the cornea istypically cooler than the iris and lens. We prescribed a temperature of34 C on the cornea and 37 C on all other surfaces.
Flow induced by saccades of the eye:We consider the flow generated in the anterior chamber by rotations ofthe eye bulb by modeling isolated rotations using the analyticalrelationship proposed by Repetto et al. (2005) which provides theangular velocity of the eye as a function of time. (bottom figure)
0
5e-10
1e-09
1.5e-09
2e-09
2.5e-09
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8
Inle
t flux [m
3/s
]
Time [s]
(a)
0
50
100
150
200
250
300
350
0 0.01 0.02 0.03 0.04 0.05 0.06
Angula
r velo
city [deg/s
]
Time [s]
(b)A= 10 deg
20 deg
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 31 / 63
Flow of aqueous humour with an intraocular lens
Flow induced by aqueous production and drainage
2e-5
4e-5
6e-5
p [mmHg]
0
7.53e-05(a)
(b)
2e-5
4e-5
U [m/s]
0
5.89e-05
Figure: Flow due to production/drainage of aqueous humorwith the device present: (a) excess pressure above IOP, (b)velocity magnitude.
0.00e+00
5.00e-05
1.00e-04
1.50e-04
2.00e-04
2.50e-04
0.06 0.07 0.08 0.09 0.1 0.11 0.12 0.13 0.14
Ave
rag
e in
let
pre
ssu
re [
mm
Hg
]
Minimum distance between the pIOL and the iris [mm]
without pIOL
pIOL without hapticspIOL
Figure: Average pressure drop between iris–lenschannel and trabecular meshwork as a function ofthe minimum distance between the pIOL and theiris (solid circles). No pIOL (horizontal line) andpIOL with haptics (empty circle).
Lubrication theory:
∆p =6µQ
π
∫ r2
r1
dr
rh3,
To get ∆p = 1mmHg you need h = 1 µm
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 32 / 63
Flow of aqueous humour with an intraocular lens
Flow induced during miosis
-0.0005
0
0.0005
0.001
0.0015
0.002
0.0025
0.003
0.0035
0.004
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8
Ave
rag
e in
let
pre
ssu
re [
mm
Hg
]
Time [s]
pIOLwithout pIOL
Figure: Spatially averaged pressure over the inlet as a function of time during miosis, in an eye both with thepIOL (solid circles) and without it (open circles).
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 33 / 63
Flow of aqueous humour with an intraocular lens
Buoyancy-driven flow I
0.0002
0.0004
U [m/s]
0
0.000592
(a)
0.0002
0.0004
U [m/s]
0
0.000585
Figure: Buoyancy-driven flow in the absence of the pIOL. Gravity acts in the vertical direction. Streamlines ofthe flow and the distribution of the velocity magnitude on selected horizontal planes, (a) without the lens, (b)with the lens.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 34 / 63
Flow of aqueous humour with an intraocular lens
Buoyancy-driven flow II
0.0004
0.0008
WSS [Pa]
0
0.00119
(a)
0.001
0.002
WSS [Pa]
0
0.00255
(b)
Figure: Buoyancy-driven flow in the presence of the pIOL. (a) Magnitude of WSS on the cornea; (b) WSS onthe iris.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 35 / 63
Flow of aqueous humour with an intraocular lens
Buoyancy-driven flow III
0.0012
0.0013
0.0014
0.0015
0.0016
0.0017
0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5
Ma
xim
um
WS
S m
ag
nitu
de
[P
a]
Minimum distance between the pIOL and the iris [mm]
without pIOL
Figure: Spatial maximum of the WSS on the cornea as a function of the distance between the pIOL and the iris,neglecting the haptics (open circles). The maximum WSS with no pIOL is shown by the horizontal line, whilstthe maximum WSS with the pIOL in its normal position and including the haptics is shown by the solid circle.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 36 / 63
Flow of aqueous humour with an intraocular lens
Flow induced by saccades of the eye
0.02
0.04
0.06
0.08
0.1
0.12
0.14
0.16
0.18
0.2
0 0.02 0.04 0.06 0.08 0.1
Ma
xim
um
WS
S m
ag
nitu
de
o
n t
he
co
rne
a [
Pa
]
Time [s]
(a)without pIOL
pIOL
0.02
0.04
0.06
0.08
0.1
0.12
0.14
0.16
0.18
0.2
0 0.02 0.04 0.06 0.08 0.1
Ma
xim
um
WS
S m
ag
nitu
de
o
n t
he
iris [
Pa
]
Time [s]
(b)without pIOL
pIOL
Figure: Time evolution of the spatial maximum of the WSS on the (a) cornea and (b) iris in an eye performinga saccade of angle 10 .
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 37 / 63
Flow of aqueous humour with an intraocular lens
Discussion and conclusions
(i) If the lens is properly placed there is a negligible influence on the pressure in the posterior chamber.
(ii) There is no significant increase of the WSS on the cornea.
(iii) The WSS on the iris is significantly greater than in the case with no pIOL, but the increase is not likely tobe sufficiently great so as to give a risk of cell detachment.
Endothelial cell loss:
Kaji et al.(2005) performed experiments on porcine corneal endothelial cells that were plated onto glassslides, and found that significant detachment was observed for shear stresses in excess of 0.03 Pa if thecells had had 1 hour of adhesion, rising to 0.1 Pa for 3 hours of adhesion. Our model predicts that theactual values are significantly smaller than this.
Other causes: rubbing the eye (mechanical forcing), insufficient delivery of oxygen and/or nutrients to thecorneal epithelium. The latter will be studied in the near future.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 38 / 63
Retinal break
Computer modeling of rhegmatogenous retinal detachment
D. Natali1, R. Repetto1, J. O. Pralits1,J. H. Siggers2 and Tom H. Williamson3
1 Department of Civil, Chemical and Environmental Engineering, University of Genoa, Italy,2 Department of Bioengineering, Imperial College London, London SW7 2AZ, UK,
3 Retina Surgery, 50-52 New Cavendish Street, London W1G 8TL, United Kingdom
A paper is in preparation for investigative ophtamology & visual science (iovs)
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 39 / 63
Retinal break
Retinal break
Rhegmatogenous retinal detachment
Occurs in approximately 1 in 10,000 of the population.
Caused by the appearance of retinal breaks in the peripheral retina
Unchecked retinal detachment is a blinding condition
There is uncertainty surrounding the mechanism of action of surgical methods.
Traction on the retina from separation of the vitreous is thought to create the retinal break
Postulated that saccadic eye movements create liquefied vitreous flow in the eye, which helpto lift the retina.
Experience says that the hole condition detaches quicker than the free flap condition
Here: use numerical simulations (FSI) to investigate the two cases under realistic conditions togive indications to surgeons.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 40 / 63
Retinal break
The model
x
y
θ θ
X(s, t)
Xp(t)
ρ, ν
Γ1
Γ2
L
∆Ω
Π
Σ∂Ωleft ∂Ωright
∂Ωtop
∂Ωbottom
n
τ
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 41 / 63
Retinal break
The model parameters
Quantity Value Reference
Properties of the retinal flap
Density ρS 1300 kg/m3
Length L 1.5− 2.5 mm yesThickness 70 µm yes
Bending stiffness Kb 2.98 · 10−11 Nm2
Young modulus E 1.21 · 103 N/m2 yes
Properties of the fluid
Density ρF 1000 kg/m3 yesDynamic viscosity µ 1.065 · 10−3 kg/ms yes
Table: Parameter values used for the simulations.
thickness/length = h/L = 1/30
The flap is considered a slender body, Kb = Eh3/12
Curvature neglected but the vitreous chamber radius ≈ 12 mm
It is known that the retina is slightly heavier than the aqueous humor,here ρS/ρF = 1.3
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 42 / 63
Retinal break
The saccadic eye movement
Saccade: Ballistic, conjugate, voluntary eye movements that change the point of foveal fixation.
(note: labels must be switched)
The saccade is modeled by a fifth polynomial, in Repetto et al. (2005)
angle is 8
The maximum velocity is 0.061 m/s
The duration is 0.045 s
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 43 / 63
Retinal break
The mathematical model
∂u
∂t(x, t) + u(x, t) · ∇u(x, t) = −∇p(x, t) +
1
Re∇2u(x, t) + f(x, t)
∇ · u(x, t) = 0(5)
Fimp = α
∫ t
0(Uib −
∂X
∂t)dt′ + β(Uib −
∂X
∂t) (6)
F = (Fimp · n)n + (Fimp · τ )τ (7)
f(x, t) = ρ
∫Γ
F(s, t)δ(x− X(s, t))ds (8)
Uib(s, t) =
∫Ω
u(x, t)δ(x− X(s, t))dΩ (9)
∂2X
∂t2=
∂
∂s
(T∂X
∂s
)−
∂2
∂s2
(γ∂2X
∂s2
)+ Fr
g
g− F (10)
∂X
∂s
∂2
∂s2
(T∂X
∂s
)=
1
2
∂2
∂t2
(∂X
∂s
∂X
∂s
)−
∂2X
∂t∂s
∂2X
∂t∂s−∂X
∂s
∂
∂s(Fb − F) (11)
along with suitable boundary conditions.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 44 / 63
Retinal break
The tendency to detach
From the solution of the FSI problem we can evaluate the forces F = (Fc,τ ,Fc,n) and themoment Mc at the clamping point(s). However, this static forces need to be combined in order toobtain an overall tendency to detach.
Here we use, from civil engineering, the Winkler’s theory. The theory assumes that the substrateon which the retinal adheres can be thought of as a system of identical, independent, closelyspaced, discrete and linearly elastic springs
kT
r(s)
s
v(s)F
Ms
v(0) =αMc + Fc,n
2α3I E, and d = max(v(0), 0),
where α ≈ 40 is the ratio between the stiffness of the substrate to the bending stiffness of thefoundation beam (retina).
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 45 / 63
Retinal break
Results I
In all cases Re = 114.6 based on maximum velocity and L = 2mm.Here, an example of the temporal variation of the wall-normal clamping force, moment andtendency to detach d .
−20
0
20
40
60
80
100
120
140
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5
0
0.2
0.4
0.6
0.8
1
t/D
Fc,n
αMc
d/dmax
Figure: The normal component of the clamping force Fc,n together with the clamping moment Mc multipliedby the parameter α on the left vertical axis and the tendency to detach d/dmax on the right vertical axis as afunction of the dimensionless time t/D. Note that d ∼ αMc + Fc,n. In this case L = 2 mm and θ = 33.56.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 46 / 63
Retinal break
Results II
Example of free-flap dynamics (giant retinal tear)
Movie 2
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 47 / 63
Retinal break
Results III
Exampel of retinal hole dynamics
Movie 2
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 48 / 63
Retinal break
Results IV
Free flap vs. retinal hole
Experience tells that the retinal hole is more prone to further detach compared to the free flap.
L = 2mm, θ = 33.56, and ∆ = 0.17mm.
0
0.5
1
1.5
2
2.5
3
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5
d/
dm
ax,fl
ap
t/D
free flap
(left side) hole
(right side) hole
Maximum value of dhole/dflap as a function of L
2.0
2.5
3.0
3.5
1.5 1.25 2 2.25 2.5
dh
ole/
dfl
ap
L [mm]
The numerical simulations are giving similar trends as what has been observed clinically with anincreasing trend for increasing values of the retinal length.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 49 / 63
Retinal break
Results V
Results regarding the free-flap case
Effect of changing the length L
0.75
1
1.25
1.5 1.75 2 2.25 2.5
dm
ax/
dm
ax,L
=2
L [mm]
Increasing tendency with increasing values of L
Effect of changing the clamping angle θ
0.5
0.55
0.6
0.65
0.7
0.75
0.8
0.85
0.9
0.95
1
15 17.5 20 22.5 25 27 30 33.56
dm
ax/
dm
ax,θ
=2
5
θ [degrees]
A maximum tendency is found for θ ≈ 25
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 50 / 63
Retinal break
Results VI
Results regarding the retinal hole
Effect of changing the length L
0.75
1
1.25
1.5 1.75 2 2.25 2.5
dm
ax/
dm
ax,L
=2
L [mm]
Increasing tendency with increasing values of L
Effect of changing the clamping angle θ
0.25
0.5
0.75
1
15 25 33.56 45 55
dm
ax/
dm
ax,θ
=3
3.5
6
θ [degrees]
A maximum tendency is found for θ ≈ 34
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 51 / 63
Retinal break
Conclusions
The tendency to detach has been analyzed both for the free flap and hole case for differentvalues of the detached retinal length, clamping angle and inter-tip distance (in the case of retinalhole). The general conclusions can be summarized as follows:
The tendency to detach of a retinal hole, compared to a retinal free flap, is 2 - 3 times largerfor retinal filaments of 1.5 - 2.5 mm, with increasing values of d for increasing values of thefilament length.
The tendency to detach increases as the retinal filament length increases, both for the retinalhole- and retinal free flap case.
A worst-case angle is found when the tendency to detach is investigated for differentclamping angles. The value is ' 25 in the free flap case and ' 34 in the hole case.
The effect of changing the inter-tip distance, which is related to the size of the retinal hole,on the tendency to detach is weak.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 52 / 63
Equilibrium shape of the aqueous humour-vitreous substitute interface
Equilibrium shape of the aqueous humour-vitreous substituteinterface
K. Isakova1, J. O. Pralits1, M. R. Romano2, J.-W. Beenakker3 and R. Repetto1
1 Department of Civil, Chemical and Environmental Engineering, University of Genoa, Italy2 Istituto Clinico Humanitas, Milano, Italy
3 Leiden University, Netherlands,
A paper is in preparation for investigative ophtamology & visual science (iovs)
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 53 / 63
Equilibrium shape of the aqueous humour-vitreous substitute interface
Equilibrium shape
Tamponade fluids (TF) used for vitrectomy are immiscible with aqueous humor (AH) and a smallpocket of AH is therefore created between the TF and the retina. Knowing the shape of theinterface and where the TF is in contact with the retina is important information for thesurgeons.
The purpose here is to predict with a numerical model the shape of the interface betweenaqueous humor and tamponade fluids in the vitreous chamber, in the case of use of a gas or asilicon oil. To determine the retinal coverage in the case of various eye shapes, from normal tohighly myopic eyes.
Infe
rior-
superior
direction
Anterior-posterior direction
T.F.
A.H.
22 mm
16 mm
R = 6 mm
Interface
Infe
rio
r-su
per
ior
dir
ecti
on
Anterior-posterior direction
The eye geometry has been taken from Atchison and Smith (2000) and the myopic eye is elongated in all
directions and dimensions are taken from Atchison et al. (2004).
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 54 / 63
Equilibrium shape of the aqueous humour-vitreous substitute interface
Mathematical model
From the Laplace-Young equation
2km = −∆ρg
γyi + B,
we see that the interface yi depends on the density difference ∆ρ, surface tension γ. Theconstant B can be found imposing the contact angle α at the solid boundary.
α
g Fluid 1
Fluid 2
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 55 / 63
Equilibrium shape of the aqueous humour-vitreous substitute interface
Mechanical properties of tamponade fluids
In this study we analyze both gas and silicone oil, both used in sugary, with values taken from theliterature.
Fluid Density(kg/m3)
Surface tension withaqueous (N/m)
Contact angle with theretina (deg)
Silicone oil 980 0.044 16.17± 1.23Intraocular gas ≈ 1 0.07 30.74± 4.24Aqueous humour 1000 - -
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 56 / 63
Equilibrium shape of the aqueous humour-vitreous substitute interface
Numerical model
The numerical, thee-dimensional steady solution is obtained using the OpenFOAM, in particular
VoF method: to keep track of the liquid-liquid interface or gas-liquid interface
snappyHexMesh: to generate the mesh (not efficient but simple)
a normal mesh has about 1.7M volumes and we run a a 32 processor work station
we integrate the governing equations in time and ”tune” the viscosity for numerical efficiency
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 57 / 63
Equilibrium shape of the aqueous humour-vitreous substitute interface
Validation of the numerical model
The numerical code was first validated for the case of sphere by comparing the results with thoseobtained by solving, analytically, the Laplace-Young equation.
(a) (b)
Figure: Shape of the interface in the spherical domain computed by analytical model (lines) and by thenumerical model (dots) for different volume fraction of tamponade fluids: silicone oil (a) and intraocular gas (b).
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 58 / 63
Equilibrium shape of the aqueous humour-vitreous substitute interface
Results I
Emmetropic (normal) eyes
Infe
rio
r-su
per
ior
dir
ecti
on
Posterior-anterior direction
(a)
Infe
rior-
super
ior
dir
ecti
on
Lateral direction
(b)
Infe
rio
r-su
per
ior
dir
ecti
on
Posterior-anterior direction
(c)
Infe
rior-
super
ior
dir
ecti
on
Lateral direction
(d)
Figure: Interface in the normal eye for the silicone oil (a)-(b) and gas (c)-(d). Three different degrees of filling- 90%, 75% and 60% for the silicone oil and 40%, 25% and 10% for the intraocular gas.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 59 / 63
Equilibrium shape of the aqueous humour-vitreous substitute interface
Results II
Myopic eyes
Infe
rio
r-su
per
ior
dir
ecti
on
Posterior-anterior direction
(a)
Infe
rio
r-su
per
ior
dir
ecti
on
Lateral direction
(b)
Infe
rio
r-su
per
ior
dir
ecti
on
Posterior-anterior direction
(c)
Infe
rio
r-su
per
ior
dir
ecti
on
Lateral direction
(d)
Figure: Interface in the myopic eye for the silicone oil (a)-(b) and gas (c)-(d). (a),(c) Intersection normal tolateral direction; (b),(d) intersection normal to anterior-posterior direction. Axial length is 26.6 mm, height andwidth are 22.75 mm.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 60 / 63
Equilibrium shape of the aqueous humour-vitreous substitute interface
Results III
Comparison normal and myopic eyes (surface)
0
0.2
0.4
0.6
0.8
1
1.2
0 0.2 0.4 0.6 0.8 1
Sta
mp
on
aded
/S
Vs.o./V
Normal eyeMyopic eye
Figure: Tamponaded surface of the retina as a function of the volume fraction for the case of silicon oil.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 61 / 63
Equilibrium shape of the aqueous humour-vitreous substitute interface
Results IV
Comparison normal and myopic eyes (angle)
Ψ
(a)
φ
(b)
Figure: Measurements of the coverage angle (a) normal to lateral direction and (b) normal to anterior-posteriordirection
0
0.2
0.4
0.6
0.8
1
1.2
0 0.2 0.4 0.6 0.8 1
ψ/2
π
Vs.o./V
normalmyopic
0
0.2
0.4
0.6
0.8
1
1.2
0 0.2 0.4 0.6 0.8 1
φ/2
π
Vs.o./V
normalmyopic
Figure: Coverage angles Ψ and Φ versus the filling ration VS.O./V in the case of silicon oil.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 62 / 63
References
References I
D. A. Atchison and G. Smith. Optics of the human eye. Butterworth-Heinemann, 2000.
D. A. Atchison, C. E. Jones, K. L. Schmid, N. Pritchard, J. M. Pope, W. E. Strugnell, and R. A.Riley. Eye shape in emmetropia and myopia. Investigative Ophthalmology & Visual Science, 45(10):3380–3386, Oct. 2004. doi: 10.1167/iovs.04-0292.
B. Lee, M. Litt, and G. Buchsbaum. Rheology of the vitreous body. Part I: viscoelasticity ofhuman vitreous. Biorheology, 29:521–533, 1992.
C. S. Nickerson, J. Park, J. A. Kornfield, and H. Karageozian. Rheological properties of thevitreous and the role of hyaluronic acid. Journal of Biomechanics, 41(9):1840–6, 2008. doi:10.1016/j.jbiomech.2008.04.015.
R. Repetto, A. Stocchino, and C. Cafferata. Experimental investigation of vitreous humourmotion within a human eye model. Phys. Med. Biol., 50:4729–4743, 2005. doi:10.1088/0031-9155/50/19/021.
K. Swindle, P. Hamilton, and N. Ravi. In situ formation of hydrogels as vitreous substitutes:Viscoelastic comparison to porcine vitreous. Journal of Biomedical Materials Research - Part A,87A(3):656–665, Dec. 2008. ISSN 1549-3296.
Jan O. Pralits (University of Genoa) Fluid Mechanics of the eye June 17, 2015 63 / 63