Final Study PDF

7/30/2019 Final Study PDF

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Final&Study&Guide&&

Microbiology&

Aspergillus Condia- FUNGIAscaris lumbricoides - Nematode- Helminthes

Ascaris lumbricoides (eggs)- Nematode-

HELMINTHES


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Balantidium coli- Phylum Cilliophora- Protozoa

Candida albicans (budding Yeast) FUNGI

Bacillus

Capsule stain


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Enterobius vermicularis - Nematode-

HELMINTHES

Clostridium

ENTERI

Endospore


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Fasciola hepatica- Trematodes- HELMINTHES

Giardia lamblia- Sarcomastigophora:Subphylum

Mastigophora - PROTOZOA

Entamoeba histolytica - Subphylum sarcodina-

Protozoa

Flagella


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Penicillium notatum- FUNGI Schistosoma japonicum -Trematodes-

HELMINTHES

Plasmodium vivax - Apicomplexans-

PROTOZOA

Nisseria


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Taenia solium- Cestodes- HELMINTHES

Schistosoma mansoni (eggs) - Trematodes-

HELMINTHESStaph

Strept


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TRICHOMONAS vaginalis -Sarcomastigophora

PROTOZOA-

Beta-hemolysin breaks down the red blood cells and

hemoglobin completely. This leaves a clear zone around

the bacterial growth.

Alpha-hemolysin partially breaks down the red blood

cells and leaves a greenish color behind

If the organism does not produce hemolysins and does not

break down the blood cells, no clearing will occur. This is

called-hemolysis (gamma hemolysis).


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Bacterial Susceptibility test on Blood Agar Bile esculin agar contains oxgall (bile salts) to inhibit

the growth of gram positive organisms other than

enterococci and group D streptococci.

NEG

POSITIVE

(MSA) is a selective and differential medium. The high concentration of salt (7.5%) selects for members of the genus Staphylococcus,

since they can tolerate high saline levels. If an organism can ferment mannitol, an acidic byproduct is formed that will cause the phenol

red in the a ar to turn ellow.


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Coagulase&Test& Urea&Test&

+& 9&=&

The coagulase test identifies whether an organism produces

the exoenzyme coagulase, which causes the fibrin of blood

plasma to clot.

differential medium that tests the ability of an organism to

produce an exoenzyme, called urease, that hydrolyzes urea toammonia and carbon dioxide.

selective and differential medium designed to isolate and differentiate

enterics based on their ability to ferment lactose. Bile salts and crystal

violet inhibit the growth of Gram positive organisms

selective and differential medium used to isolate fecal coliforms

Green=fermentation+acid Pink= Fermentation little acid


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Indole&test&

+ = N/R&

Glucose&Fermenta@on&

Methyl&Red&Test&SIM&Tubes&

Sim Medium used to detect enterics

SIM medium is a combination differential medium that tests three

different parameters, which are represented by the three letters in the

name: Sulfur Reduction Indole Production Motility

The Methyl Red test

involves adding the pH

indicator methyl red to an

inoculated tube of MR-VP

broth. If the organismuses the mixed acid

fermentation pathway and

produces stable acidic

end-products, the acids

will overcome the buffers

in the medium and

produce an acidic

environment in the

medium. When methyl red

is added, if acidic end

products are present, the

methyl red will stay red.


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Starch agar is a differential medium that tests the ability of an organism to produce certain

exoenzymes, A clearing around the bacterial growth indicates that the organism has

hydrolyzed starch.

NO REACTION

POSSITIVE

+ IODINE

The oxidase test identifies organisms that produce the enzyme cytochrome

oxidase. If the test organism produces cytochrome oxidase, the oxidase reagent

will turn blue or purple within 15 seconds.

used to detect acetoin in a bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth which has been

inoculated with bacteria. A cherry red color indicates a positive result, while a yellow-brown

color indicates a negative result.


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Lactose&Fermenta@on&

+&Gas&&&&&&&&&+&&&&&&&&&&&&&&&=&

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