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FINAL REPORT National Agricultural Innovation Project (Indian Council of Agricultural Research) Title of Sub-project: A Value Chain on Murrel Production in Tamil Nadu and Orissa Lead Consortium Centre for Aquaculture Research and Extension (CARE) St. Xavier's College (Autonomous) Palayamkottai, Tamil Nadu - 627 002 2014 A Value Chain on Murrel Production in Tamil Nadu and Orissa Component-2 2014 2014

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Page 1: FINAL REPORT - ICARnaip.icar.gov.in/download/c2-206101.pdfPro-forma: 1. Details of Technologies Developed/ Validated/ Adopted ... Baseline Information on the Pre- Project Situation

FINAL REPORT

National Agricultural Innovation Project

(Indian Council of Agricultural Research)

Title of Sub-project:

A Value Chain on Murrel Production in Tamil Nadu and Orissa

Lead Consortium

Centre for Aquaculture Research and Extension (CARE)

St. Xavier's College (Autonomous)

Palayamkottai, Tamil Nadu - 627 002

2014

A V

alu

e C

hai

n o

n M

urr

el P

rodu

ctio

n i

n T

amil

Nad

u a

nd

Ori

ssa

Component-2

2014

2014

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Page 3: FINAL REPORT - ICARnaip.icar.gov.in/download/c2-206101.pdfPro-forma: 1. Details of Technologies Developed/ Validated/ Adopted ... Baseline Information on the Pre- Project Situation

FINAL REPORT

National Agricultural Innovation Project

(Indian Council of Agricultural Research)

Title of Sub-project:

A Value Chain on Murrel Production in Tamil Nadu and Orissa

Lead Consortium

Centre for Aquaculture Research and Extension (CARE)

St. Xavier's College (Autonomous)

Palayamkottai, Tamil Nadu - 627 002

2014

A

Val

ue

Ch

ain o

n M

urr

el P

rodu

ctio

n i

n T

amil

Nad

u a

nd

Ori

ssa

Component-2

2014

Page 4: FINAL REPORT - ICARnaip.icar.gov.in/download/c2-206101.pdfPro-forma: 1. Details of Technologies Developed/ Validated/ Adopted ... Baseline Information on the Pre- Project Situation

FINAL REPORT

National Agricultural Innovation Project

(Indian Council of Agricultural Research)

Title of Sub-project:

A Value Chain on Murrel Production in Tamil Nadu and Orissa

Lead Consortium

Centre for Aquaculture Research and Extension (CARE)

St. Xavier's College (Autonomous)

Palayamkottai, Tamil Nadu - 627 002

2014

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Inside Cover Page

Printed on :

Component : 2

Citation :

Copyright : Indian Council of Agricultural Research (ICAR)

Disclaimer :

Published by : Name, Designation, Address, Phone, Fax, E-mail, Website

Edited by :

Compiled by :

Hindi translation :

Other Credits :

Cover page

photographs

:

______________________________________________________________________________

Printed at

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CONTENTS

Page Number

Foreword -by CL

Preface -by CPI

1

Executive Summary/ Key words 2-5

Part-I: General Information of Sub-project 6-7

Part-II: Technical Details

1. Introduction 8

2. Overall Sub-Project Objectives 9

3. Sub-Project Technical Profile 9-11

4. Baseline Analysis 11-12

5. Research Achievements 12-30

6. Innovations 30-33

7. Process/Product/Technology/Value Chain/Rural Industry

Developed

33

8. Patents (Filed/Granted) 34

9. Linkages and Collaborations 34-35

10. Status on Environmental and Social Safeguard Aspects 35

11. Constraints, if any and Remedial Measures Taken 35-36

12. Publications 36-45

13. Media Products Developed/Disseminated 45-47

14. Meetings/Seminars/Trainings/Kisan Mela, etc. organized 47-48

15. Participation in Conference/ Meetings/Trainings/ Radio talks, etc. 49-53

16. Trainings/Visits undertaken (National/ International) 53

17. Performance Indicators 53-54

18. Employment Generation 54

19. Assets Generated 54-60

20. Awards and Recognitions 60-61

21. Steps Undertaken for Post NAIP Sustainability 61-62

22. Possible Future Line of Work 62

23. Personnel 62-63

24. Governance, Management, Implementation and Coordination 64-66

Part-III: Budget and its Utilization 67

Part-IV: Declaration 68

Pro-forma:

1. Details of Technologies Developed/ Validated/ Adopted

2. Details of Technologies/ Innovations Commercialized

3. Details of Rural Entrepreneurships/ Rural Industries Developed

Annexure:

1. Publications

2. Murrel Value Added Products

3. Linkages andCollaboration

4. Success Stories

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FOREWORD

Aquaculture has been recognized as a powerful income and employment generator which

stimulates growth of a number of subsidiary industries. It is the only sector which offers cheap

and good animal protein to the people, particularly to the economically weaker section of the

society and thereby serving as a mean ensuring national food security. No doubt, demand for

edible fish is increasing day by day and the only solution is culture practices in freshwater,

brackishwater and sea which promise to meet our increasing fish demand. At present, availability

of fishes in the markets shows a dwindling trend and the supply from the wild and capture

fisheries is not enough to meet the consumers demand. At this juncture, inland fish culture

especially murrel culture, native catfish and eel culture may be fruitful to fish consumers.

I am happy to note that considering the present needs, the Director, Dr.M.A.Haniffa and

his research team of Centre for Aquaculture Research and Extension (CARE) of St.Xavier‟s

College (Autonomous), Palayamkottai, have undertaken extensive investigations on murrels and

native catfishes. I am proud to state that CARE is the unique centre internationally recognized

for research in murrel farming, transfer technologies and conservation of threatened freshwater

fishes by availing financial assistance for 26 major research projects from ICAR., UGC, CSIR,

DST, DBT and MOE since 1980.

I am indeed very happy to know that Dr.M.A.Haniffa, Director CARE produced more

than 40 Ph.Ds and among them, 18 Ph.Ds exclusively on murrel research. It is also gratifying to

note that considering monopoly in murrel research, Dr.Haniffa was given a sub project on “A

Value Chain on Murrel Production in Tamil Nadu and Orissa (F.No. 1(5)/2007-NAIP dt. 22

August 2008)” by ICAR under NAIP to the tune of Rs.4 Crores. I am happy to know that Centre

for Aquaculture Research and Extension, the lead Consortium was given Best Appreciation

Certificate during the National meet on “Technological Innovations in Agriculture”, held at

NASC Complex, New Delhi from 21-22 May 2010.

Under the NAIP sub project, Dr.Haniffa and his research team produced 24 Video CDs,

published 39 articles and two books. Moreover, Two patents on „Murrel Cookies‟

(1227/DEL/2013 dt.30.04.2013) and „Murrel Dhal Powder‟ (3168/DEL/2013 dt.24/10/2013)

have been filed. Meanwhile, popularization of murrel fish foods has been made through three

festivals (Murrel Fish Food Expo‟ 2012, Nellai Fish Food Festival‟ 2013 and Nanjil Fish Food

Festival‟ 2013). Stakeholders including housewives, Fish Farmers, Fish Vendors, Self Help

Groups and Voluntary Organizations were motivated to start murrel culture by 15 training and 7

workshop.

I congratulate Dr.M.A.Haniffa and his research team for their commendable contribution

to Value Chain of Murrels in the Global scenario.

Rev.Dr.V.Gilburt Camillus, S.J.,

Consortium Leader and Principal,

St.Xavier‟s College (Autonomous),

Palayamkottai, Tamil Nadu

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PREFACE

Air breathing fishes form about 13% of marketable freshwater fishes in India and among

them, murrels commonly called snakeheads are highly priced due to taste, less intramuscular

spines and medicinal and therapeutic values. Among the murrels, the giant murrel Channa

marulius, the striped murrel Channa striatus, the spotted murrel Channa punctatus, and redline

murrel Channa diplogramme are widely preferred as edible fish due to their large size. Fish

farmers in India mostly culture major carps viz: rohu, catla and mrigal. Moreover, major carp

seeds have been introduced into lakes, ponds, and reservoirs in millions in India. Hence, major

carps are always available in local markets all over India, eventhough they fetch a very low price

and less consumer preference. Moreover, at present our fish farmers are crazy in exotic catfish

(Thai catfish and African catfish) culture eventhough they pose a great threat to biodiversity of

freshwater fishes. In India, established model farms are flourishing for herbivorous carps and

omnivorous penaeid shrimps, but such a system is lacking for air-breathing fishes especially

murrels.

The value chain of murrel farming is definitely an alternative choice for usual carp

culture and Prawn culture to get more profit as well as conservation of biodiversity. At this

crucial juncture, murrel farming will not only enhance the socio economic status of fish farmers

of our country but also help conservation of existing fish biodiversity. Unlike other culture

practices, murrel farming is ecofriendly without polluting land and water resources. Actually,

murrel farming is a very important value chain in Indian scenario and till date, there is no

difficulty in marketing of murrels due to consumers demand. Unfortunately, Indian fish farmers

are unable to culture murrels due to non availability of seeds as well as feed and lack of

knowledge on seed and feed production. Moreover, murrels are highly prone to diseases

especially Epizootic Ulcerative Syndrome (EUS). They depend on fishermen for seed collection

from the wild, which is unpredictable due to monsoon failure. Hence seed production by

artificial means is necessary for availability of seeds throughout the year.

In recent years, Indian Government put a ban on fishermen to capture marine fishes for a

period of 45 days each in east coast and west coast. This creates not only non availability of

marine fish in the markets but also unacceptable escalation of price cautioning the need for

culture fisheries especially murrel farming to satisfy fish consumers.There is an urgent need to

popularize murrel culture among stakeholders by overcoming the constraints which could be

achieved by training and workshop by providing informations on seed production, larviculture,

feed formulation, live feed culture, growout culture, and prophylaxis and treatment of diseases.

I express my sincere thanks to Indian Council of Agricultural Research, New Delhi for financial

assistance to the tune of Rs.4 crores to carry out the present project on “A Value chain on Murrel

Production in Tamil Nadu and Orissa”. My thanks are due to Prof Dr.T.J Pandian, National

Professor and the Management of St.Xaviers College for encouragement and support.

I believe that this report would be of immerse help to Researchers, Fish Farmers, Fish

Vendors, SHG‟s, Policy Makers, Organisations and Unemployed youths.

Dr.M.A.Haniffa, PI

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Executive Summary

Executive summary

Background Information:

In our country, carp culture is promoted everywhere whereas air breathing fish culture is

neglected. Among the air breathing fishes, murrels occupy the top most rank by fetching the

highest price (Rs. 500 – 700/-) due to taste, fewer intramuscular spines and medicinal quality but

farmers are unable to practice murrel culture due to lack of knowledge on seed and feed

production and disease management. Since murrels are air breathing, our shallow freshwater

systems are highly suitable for murrel culture. Fish farmers prefer African catfish and Thai

catfish for culture due to short term benefit even though they pose great threat to existing

biodiversity of freshwater fishes. At this juncture, diversification of carp culture by murrel

culture will definitely be an alternative to fish farmers to earn more profit.

Baseline Information on the Pre- Project Situation in the Project Area:

Baseline survey indicated that carp culture is practiced widely not only in Tamil Nadu but

all over India. Moreover, fish farmers are not familiar to murrel seed production, and culture

which are in high demand in whole South East Asia. In India, consumers demand for murrels is

more but their availability is not only less but also seasonal. This situation warrants the need of

murrel culture and the supply of murrels to markets for consumers.

Baseline survey also showed that the consumers prefer murrels as live fish and fish

vendors often meet huge loss due to heavy mortality. Moreover, they were not familiar to

preservation of murrels or processing of murrels. Fish farmers who practiced murrel culture often

met huge loss due to sudden outbreak of Epizootic Elcerative Syndrome (EUS) and they were

not familiar to prophylaxis or treatment of murrels. In recent years, Indian Government put a ban

on capture marine fisheries for a period of 45 days each in East Coast and West Coast resulting

in non availability of sea fish as well as unacceptable escalation of their price.

Work Proposed and its Execution Plan:

The present NAIP sub project was proposed

1. To refine and replicate mass seed production techniques to increase survival and upscaling of

murrel seed production at farmers level.

2. To standardize the procedure and process of producing mass culture of live feed organisms

and formulation of microencapsulated diet and semi moist feed for growout culture of

murrels.

3. To enhance the knowledge, skills and attitude of stakeholders in sustainable use of

stakeholders ponds, for murrel farming for their socio economic upliftment by training and

workshop.

4. To strengthen weaker chains like disease management and rectification of missing links viz :

transport as frozen/chilled flesh, preparation of fillet for ready to cook and ready to cook

products and application of anaesthetics for live fish transport.

5. To evaluate the medicinal value of murrels, herbal and probiotics incorporations in diets as

immunostimulators and growth promoters and to popularize murrel value products through

workshop and training.

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3

Achievements:

1. Production technologies developed and adopted:

Seed Production Technology:

HCG was recommended to stakeholders for upscaling of murrel (Channa striatus) seed

production in their ponds. A single breeding set of striped murrel injected with HCG (2000

IU/Kg) produced 8000-12000 eggs. 1,07,889 murrels seeds were produced by St. Xavier‟s

College, Lead Consortium between 2009 and 2012. CIFA Bhuvaneswar, produced 2,63,258

murrel seeds from 2009 to 2013 and stakeholders produced 1,10,000 seeds.

Live Feed Culture & Feed Formulation Technology:

Rotifers were found to be an excellent feed for rearing post larvae and fry especially

during early stages while the cladocerens - Moina and Daphnia were recommended for

advanced fry. For mass culture of cladocerens, chicken manure was recommended as suitable

medium at 700ppm to produce 10,000 Ind./l. Similarly, copepods and rotifers were produced at

7,000 Ind./l and 10,000 Ind./l in fibre tanks of 10,000 l capacity.

Since there is no readymade feed for murrel in markets due to their carnivorous,

piscivorous and cannibalistic behaviour, NAIP sub- project research team formulated diet

containing fish meal (anchovy and jawala) : 60%, wheat flour :10%, rice flour : 10% and soya

flour-20% respectively with 43% of protein was prepared for murrels. Feed pellets prepared

using pelletizer ranged between 2.5-5.0 mm were readily accepted by murrel fingerlings.

Larviculture Technology:

Culture performance of larval snakehead (Channa striatus) was enhanced by feeding

them with cladocerans (Ceriodaphnia cornata, Moina micrura and Daphnia carinata), and

Artemia nauplii as individual and mixed diets. It was noticed that cannibalism was reduced at

different stages by providing prey of suitable size to the growing fish.

Incorporation of herbs Phyllanthus niruri, bacterium Bacillus subtilis, Almond

Terminalia catappa and aloe Aloe vera in diet resulted in better growth performance and

immunostimulation in Channa striatus fingerlings.

Growout Culture Technology:

Earthern ponds ranging from 0.1 ha to 0.4 ha with a depth of 1m with a stocking density

of 1/m2 were recommended for murrel culture. Usually, the culture period is about 8-10

months to obtain table size fish of 750g weight.

52 farmers of Tamilnadu harvested 27.67 tons of murrels and the harvest ranged from 0.1

ton – 5 tons/ha/yr with a profit range of Rs.30,000 to Rs. 15,00,000/ha/yr in large scale murrel

farming; whereas in small scale murrel farming using cement tank, in an area of 1,000 sq. feet,

one stakeholder produced 400 murrels (300kg) fetching Rs.1,50,000/ha/yr a breakthrough in

the histry of murrel culture

CIFA, Bhubaneshwar showed production of 1 ton/ha/yr -2.5 tons/ha/yr fetching a profit

ranging from Rs. 1.25 lakhs to Rs. 3.12 lakhs/ha/yr.

Murrel Disease Management Technology:

Regarding murrel disease management, crude extract of medicinal herbs - Wrightia

tinctoria was successfully used to prevent EUS occurrence and enhanced survival during

larviculture and growout culture. W. tinctoria was recommended to fish farmers due to the

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antibacterial and antifungal activities against pathogens (pseudomonas fluorescens, Aeromonas

hydrophila, Staphylococcus epidermidis, Aeromonas salmonicida, Aphanomyces invadans and

vibrio alginolyticus. Other measures for treatment of EUS included the application of medicinal

plant Coleus aromaticus and herbs, Ocimum sanctum (Tulsi), Aegel marmelos (stone apple) and

rhizome of Curcuma longa (turmeric).

Murrel Transport Technology:

For live murrel transport, 400-500 ppm of clove oil was recommended as sedative since

these concentrations do not cause irreversible changes on hematological/biochemical profiles

in Channa striatus. Aluminium drums (1.75*2*6 ft) of 100 l capacity suggested for transport

of 45 adult murrels (25 Kg) for 24 hours travel.

2. Process Technologies developed, adopted and commercialized:

Murrel Filleting Technology:

Consumers always prefer murrels as live and fish vendors meet heavy loss due to

mortality of murrels. Hence filleting technology was recommended to fish vendors to supply

fillets to consumrs for preparation of ready to eat and ready to cook products.

The CARE research team has filed two patents for Murrel Cookies and Murrel Dhall

Powder; Third patent on Murrel – Millet Mix in collaboration with Department of Food Science

and Nutrition, University of Agricultural Sciences, Bangalore is under progress.

Human Health Management Technology

Murrels are widely preferred due to their medicinal value and recommended for treatment

of wound, ulcer, convalescence and arthritis. Muscle extracts of murrels showed antibacterial

activity against the enteric pathogens showing the zone of inhibition ranging from 7.0 mm -

24.6mm. Skin and Intestinal mucus of murrels also exhibited antibacterial activity.

The PUFA/ Saturated (P/S) ratio of Channa striatus fish oil is 0.69 which is higher than

the patin (Pangasius hypothalamus) with 0.67. On the otherhand, the ω-3/ω-6 ratio of Channa

striatus is 0.96 which is higher than the catfish, Clarias macrocephalus (0.25) and Patin (0.73)

indicating high nutritional value of murrel fillets suitable for children and old people.

Wound healing studies using wistar rats showed faster wound healing upon topical

application of Channa striatus crude extracts. Our results revealed that murrels are bio assets to

the country.

3. Rural industries established/commercialized: Nil

4. Patents (filed/granted):

Nutritional Cookie Composition and A Process for Preparing the Same; Filed (

1277/DEL/2013dt. 30.04.2013)

Process for Preparing Nutritionally Rich Dhal Powder and Its Composition; Filed

(3168/DEL/2013 dt.24/10/2013)

5. Publications: (Please refer Annexure I)

Research papers published: 23

Popular articles published: 14 (in English), 10 (in otherlanguages)

Books/book chapters published: Books: 4; Book Chapters: 9

Bulletins/brochures/leaflets published: 2

Training manuals published: 6

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5

Film / CDs developed: 25

Coverage in press, TV, media: Newspaper articles: 40

6. Trainings undertaken and scientists/other staff trained (national/international): Nil

7. Trainings organized and farmers/other stake holders trained: 1119 farmers through 21

training and 12 workshop (Please refer pages 47-48)

8. Field demonstrations organized: 21 Training and 12 Workshop (Please refer pages 47-48)

9. Field day/farmer day/‟mela‟ organized: 4 (Please refer page 48)

10. Success stories: 16 (Please refer Annexure IV)

11. Self help groups/farmer groups developed: 11 (Please refer Annexure III)

12. Employment generation (man days/year):

S. No. Type of Employment Generation Employment Generation

up to End of Sub-project

Responsible

Partner

1. Seed production and Murrel farming by

farmers (entrepreneurship / self-

employment)

3,139 CIFA,

Bhubaneswar

2. Engagement of manpower (workman)

for murrel farming (man-days/year)

11,680 CIFA,

Bhubaneswar

13. Assets generation (equipments/implements procured, civil work done and revenue

generated):

Please refer Pages 54-60.

S. No. Source of Revenue Year Total amount

(Rs.`)

Responsible

Partner

1. By Supply of Murrel Fingerlings 2009 -

2010

5000 Lead Consortium –

CARE,SXC

14. Awards/honors: 12 (Please refer pages 60-61)

Key words:

Channa striatus, C. marulius, Murrel, Seed Production, HCG, Live Feed, Zooplankton,

Ceriodaphnia cornata, Miona micrura and Daphnia carinata, Larviculture, Pelleted Feed,

Growth Promotors, Immunostimulators, Growout culture, Fillet, Murrel Value Addition, Murrel

Cookies, Murrel Dhal Powder, Murrel Gel, EUS, Training, Workshop, Expo, Linkage, Live

Murrel Transport, Anesthesia and Murrel Medicinal Value.

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Part-I: General Information of Sub-project

1. Title of the sub-project: A Value Chain on Murrel Production in Tamil Nadu and Orissa

2. Sub-project code: 4059

3. Component: 2

4. Date of sanction of sub-project: 22nd

August 2008

5. Date of completion: 30.06.2012

6. Extension if granted, from 01.07.2012 to 31.03.2014

7. Duration of the sub project: 01.09.2008 to 31.03.2014 (Five years and seven months)

8. Total sanctioned amount for the sub-project: Rs.454.76/- lakhs

9. Total expenditure of the sub-project:Rs.405.11589/- lakhs

10. Consortium leader:

(Name of CL, Designation, Organization Address, Phone & Fax, E-mail, Website)

St. Xavier's College (Autonomous), Palayamkottai, Tamilnadu - 627 002

Phone: 0462-2560744/4264301; Fax: 0462-2561765;

Email: [email protected]; Website: www.stxavierstn.edu.in

Name Duration

1) Rev.Dr.S.Alphonse Manickam S.J. ,Principal CL November 2008- May 2012

2) Rev.Dr.A.Joseph S.J., Principal CL June 2012- May 2013

3) Rev.Dr.V.Gilburt Camillus S.J., Principal CL May 2013 – March 2014

11. List of consortium partners:

Name of CPI/

CCPI with

designation

Name of organization and address,

phone & fax, email

Duration

(From-To)

Budget

( Lakhs)

CPI Dr.M.A.Haniffa

Director

Centre for Aquaculture research and

Extension, St. Xavier's College,

Palayamkottai, Tamilnadu - 627 002

Phone: 0462-2560670 Fax: 0462-

2560670

Email: [email protected]

Website: www.caresxc.org

November 2008 -

March 2014

316.010

CCPI1 Dr.A.John De

Britto

Associate

Professor

Department of Plant Biology and

Biotechnology, St. Xavier's College

Phone:0462-2560813

Fax: 0462-2561765

Email: [email protected]

November 2008 -

March 2009

1.39925

CCPI2 Dr.K.Altaff

Principal

The New College (TNC), Chennai,

Tamil Nadu 600014.

Phone: 044-28351050;

Email: [email protected]

November 2008-

March 2012

26.191

CCPI 3 Dr.A.K.Sahu Central Institute of Freshwater 2008-September 2013

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Principal

Scientist

Aquaculture (CIFA), Kausalya Ganga,

Bhubaneshwar

Phone: 0674-2465446

Email:[email protected]

[email protected]

[email protected]

116.69

CCPI 4 Dr.Kuldeep

Kumar

Senior Scientist

2008-March 2013

CCPI 4 Dr.Rajesh

Kumar

Scientist

2008-September 2013

as Co-PI and October

2013-March 2014 as

CCPI

CPI-Consortia Principal Investigator; CCPI-Consortia Co-Principal Investigator

12. Statement of budget released and utilization partner-wise (` in Lakhs):

CPI/ CCPI Name, designation &

address)

Total

budget

sanctioned

Fund released

(up to closing

date)

Fund utilized

(up to closing

date)

CPI Dr.M.A.Haniffa 317.41 287.11776 285.004

(* Upto 15th

Jan 2014)

CCPI1 Dr.K.Altaff. 26.191 20.72806 22.67189

CCPI 2 Dr.A.K.Sahu,

Dr. K. Kumar, Pr. Scientist

Dr. A. K. Sahu, Pr. Scientist

Dr. Rajesh Kumar, Scientist

CIFA, Kausalyaganga,

Bhubaneswar- 751002, Odisha

116.69 99.73 97.44*

(*Up to 15th

Jan 2014)

Total

CPI-Consortia Principal Investigator; CCPI-Consortia Co-Principal Investigator

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Part-II: Technical Details

1. Introduction (Give background information for taking up this sub-project)

Our country is gifted with ponds and tanks covering 2.36 million hectares (ha), but 0.8-0.9

million ha only has been utilized for aquaculture that too mostly for carp culture. In 2008, the

annual demand for carps and air breathing fishes in Tamil Nadu is about 6-7 lakhs tons and 3-4

tons respectively. But the inland fish production in Tamil Nadu was estimated as 1,60,172 tons/

year. Out of this air breathing fishes such as murrels and catfishes comprise hardly less than

10%. When compared to annual demand for murrels of 3 lakhs tons, the existing supply by

capture fisheries was about 0.16 lakh tons only (< 20%) in 2007-2008. Air breathing fishes form

about 13% of the marketable surplus of freshwater fishes in India and among them murrels are

highly priced (Rs.100-200/kg in 2007-2008; Rs.200-300/kg in 2008-2009; Rs.400-450/ kg in

2010-2011; Rs.550/kg in 2012 and Rs.600-700/kg by 2014) for their quality, taste, flavour and

nutritive, recuperative and medicinal properties in the states of Tamil Nadu, Assam, Punjab,

West Bengal and Madhya Pradesh (CIFRI Bulletin No.53, 1988). Considerable scope exists for

augmenting the supply of murrels to markets/ tourist hotels and ultimately exports by

undertaking large scale culture in our country.

Murrels are air breathers and survive in oxygen depleted water bodies and hence are suitable

for profitable culture in shallow systems. Murrels are sold immediately after capture as live fish

due to consumer‟s preference. About 28 to 30 Channa species have been reported in the global

scenario and 8 to 10 species in India. Giant murrel and striped murrel are regarded as excellent

table fish due to their large size in India as well as in South East Asia. Unfortunately, Indian fish

farmers are unable to culture murrels due to non availability of seeds as well as feed. Moreover,

larviculture of murrels is a Herculean task, since murrels are Carnivorous, Piscivorous and

Cannibalistic. When compared to Thailand, Malaysia, Vietnam and Philippines, we are far

backward in murrel farming. Regarding Production to Consumption System (PCS), the present

supply of murrels from the wild does not fulfill the demand of consumers. Hence, the present

project deals with refinement of seed production techniques by R&D and upscaling of seed

production in stakeholder‟s ponds. It also deals with larviculture of murrels by live feed culture

(Daphnia, Moina and Fairy Shrimp) and microencapsulated diet. Since, growout culture was not

practiced due to lack of readymade feed, it was proposed to formulate semi-moist feed using

agro-waste (chicken intestine, trash fish and fish waste) for murrel culture. The fish farmers who

have attempted murrel farming often encounter failures due to sudden outbreak of diseases like

Epizootic Ulcerative Syndrome (EUS). Hence, it was proposed to supplement the feed for young

ones as well as adults by incorporating medicinal herbs and probiotics as immunostimulants and

growth promotors. The preference for dead murrels is less and hence value addition of murrels is

proposed for income generation. The outcome of this unique value chain on murrels by

production of seeds and table size fish will definitely help the socially weaker sections especially

the fish farmers and unemployed youths to generate income and to overcome malnutrition and to

conserve the threatened murrel species due to anthropogenic stresses by captive breeding.

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2. Overall Sub-project Objectives

Refinement of techniques for mass seed production of murrels (R&D)

Standardization of larviculture techniques (hatchling to post larva and postlarva to fry and

fingerling) (R&D)

Mass culture of live feed (Moina, Daphnia and fairy shrimps) and microencapsulated diet a

vital link for mass production of murrels (R&D).

Formulation of semi-moist feed by incorporating medicinal herbs and probiotics (R&D)

Standardization of semi-moist pelleted feed using chicken intestine, trash fish and fish

waste (R&D)

Organization of target community into Self Help Groups and their network for production

and marketing

Transport of live murrel fish to nearby markets and processed ones (as frozen) to

other states and ultimately for export

Popularization of murrel farming among stakeholders for income generation by training and

workshop

Objectives for Extension Period

Preparation of murrel fish oil for treatment of arthritis, wound healing and convalescence

Antimicrobial and Antinociceptive properties of mucus, oil and fillets

Murrel cetrimide cream for treatment of skin infection

Preparation of Gelatin from murrel skin

Preparation of Omega-3 Syrup using murrel fillets

Preparation of packed food products viz ready to serve products (e.g. Murrel fish biscuits and

cakes, Murrel sandwiches) and ready to cook products (e.g. Murrel-millet mix).

Soup preparation from head, bones and fins of murrels

Utilization of murrel skin in wallet and footwear industries

Preparation of manure using fish wastes

3. Sub-project Technical Profile

(Indicate briefly objective-wise work plan, monitoring indicators, expected output and

expected outcome)

S.

no

Major activities

Striped murrel - C. striatus and

Giant murrel- C. marulius

Verifiable Indicators 1st

Yr

2nd

Yr

3rd

Yr

4th

Yr

5th

Yr

1 2 1 2 1 2 1 2 1

1 1.1 Collection of murrel brood stock

C. striatus & C. marulius from

rivers and wetlands of Tamil

Nadu and Kerala

1.2 Brood stock rearing

No. of brood stock

collected and reared.

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1.3 Using the perfected techniques

of induced breeding and artificial

fertilization in C. striatus & C.

marulius

Adequate no. of fertilized

eggs generated/year and,

upscaling of seed

production at fish farmer‟s

level.

1.4 Larviculture techniques for C.

striatus and C. marulius

No. of C. striatus and C.

marulius fingerlings

produced and amount of

daphnia and moina

produced.

2 2 Demonstration of growout

culture of murrels in 22 farm ponds

Annual harvest of striated

and giant murrels in

number and weight;

percentage of survival. 30,

000 murrels (25 tonnes)

and ultimately 100 tonnes.

3 3.1 Use of medicinal herbs

incorporated in murrel feeds as

immunoprophylactics by

Department of Plant Biology and

Biotechnology, St.Xavier‟s

College.

Periodical growth

measurements; periodical

health assessment by

recording mortality and

disease.

3.2 Use of poultry waste (chicken

intestine) and fish waste as major

ingredients and to formulate a feed

in semi moist form to ensure fastest

growth and survival

Gut content analysis;

periodical measurements;

food conversion ratio

3.3 Transport of fish as live and

frozen

No of fish transported and

sold

4 Training researchers, fish farmers

and other stakeholders.

No. of stakeholders/

trainers trained/ year

No. of stakeholders starting

murrel culture/ year

Production of Pamphlets,

Manuals, Video CDs and

DVDs

Output Outcome*

Production of 30,000 post larvae at SXC and 1,00,000 –

10,00,000 murrel seeds from the second year to fourth

year in fish farmers ponds

Refinement of mass seed

production techniques and

upscaling of seed production at

farmers level and development of

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larviculture techniques

Mass production of live feed (1kg -5kg/day) from the

second year to fourth year at CARE Aqua farm

Refinement of mass culture

techniques of daphnia, moina and

fairy shrimp and production of

microencapsulated diet.

Standardization of semi-moist pelleted feed by using

chicken intestine and fish waste and application of

medicinal herbs as growth promoters and immune-

modulators

Production of semi moist pelleted feed 5kg/day for fry

to 50 kg/day for grow out at CARE and supply to feed

murrels in farm ponds

Transport of murrels as live fish as well as frozen flesh Transport of live fish using

anesthetics and dead murrels as

fillet

One hundred fish farmers of Tamil Nadu and

Bhubaneswar will be trained every year

Training fish farmers for seed

production and larval rearing and

growout culture

Murrel farming techniques by fish farmers and

stakeholders; 30000 of striped murrels (25 tons) in the

second year initially and 100 tons of striped and giant

murrels in the last year in Tirunelveli district and 20000

giant murrels (20 tons) in Orissa in the second year and

ultimately 100 tons in the last year

*Please refer section 5 for details of outcome of the sub-project from 2009- 2014

4. Baseline Analysis

(Baseline data on the pre-project situation supported by tables, charts, photographs)

The aim of the present project is to popularize murrel culture by identifying and rectifying

the various missing links. The data were collected through personal interviews from farmers of

Tamil Nadu and Khurda, Puri and Nayagadh districts of Orissa using well-structured

questionnaire during initial period of the project. The cost of murrel/kg varied from Rs.150/- to

Rs.200/- and above in our project operational area. The price of murrel varied with respect to the

seasons also. The price of murrel was cheaper in summer season and higher in rainy season.

39.16% respondents stated that the normal price of murrel/kg costs about Rs. 150/-, 32.5%

reported that the cost comes around Rs.200/- and 28.33% stated that the price exceeds beyond Rs

200/- (Table 1).

Regarding the availability of murrel seeds for culture, out of 120 respondents, only 30%

practice murrel culture and among them 18.33% get murrel seeds from the nearby areas, 8.33%

from natural ponds and only 3.33% produce murrel seeds of their own (Table 2). This indicates

that although murrel culture is a profitable business, the fish farmers were not aware of seed

production and murrel culture techniques.

Among the respondents, 49.17% have stated that the main problem in murrel culture is the

non availability of seeds. 39.17% have reported that they are unaware of murrel culture

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Primary Occupations

Fisheries

Animal husbandry

Agriculture

Agri. labor

others

Ownership of the ponds

Private

Community

Both Priv.& Comm.

No Pond

techniques and 11.66% have mentioned that they are not aware about the diseases and treatment

measures.

Table 1: Cost of murrel in selected districts Table 2: Availability of murrel seeds

Murrels are edible for their low fat, high protein and fewer intra muscular spines and

medicinal qualities. Unlike other fishes, the value added products derived from murrels were not

popularly known among the public and farmers. Murrel value added products were also not

available in the market. In this regard, the baseline survey undertaken among the 120

respondents indicated that only 20% were aware of murrel value added products and remaining

80% have no idea about murrel value added products.

About 55% of the respondents were literate and most of them had primary education. The

primary occupation of the respondents was agriculture (33%), agriculture labourer (36%),

fisheries (9%), animal husbandry (5%) and others. This indicated that most of the respondents

were dependent on the agricultural activity. Though they have very good resources in the form of

ponds and tanks, the fishery activity was comparatively less. Some of the fish farmers were

involved in the rearing and sale of carp fry and fingerlings. Most of the farmers in Khurda and

Puri District of orissa are small or marginal farmers. About 48% of the respondents were having

their own private ponds and most of them are using it for carp seed rearing and culture in

conventional way. The community ponds were owned by 20% of the respondents. Most of the

community ponds were leased out by women self-help groups (WSHG).

5. Research Achievements with Summary

(Give detailed technical progress partner-wise, highlighting the achievements in terms of

targets fixed for each activity and the impact of sub-project. Present statistically analyzed data

through tables/graphs. Attach good quality photographs. Present overall research achievements

Cost in

Rs.

Respondents

No of persons %

150 47 39.16

151 – 200 39 32.50

> 200 34 28.33

Total 120 100

Source Respondents

No of persons %

Supply centres 22 18.3

Production by own 4 3.33

Natural ponds 10 8.33

Total 36 100

Fig. 1 Fig. 2

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with critical discussion of results and conclusions, as if for publication in a journal. Explain the

variation in light of the scientific technological trends, socioeconomic and agro-ecological

conditions)

1. Seed Production and Upscaling

Responsible Partner: CARE, St.Xavier‟s College, (SXC), Tamil Nadu, Lead Institute &

CIFA, Bhubaneswar, Partner Institute

Murrels commonly called snakeheads fetch the highest price (Rs. 500 -700/Kg) among

freshwater fishes due to taste, less intra muscular spines and medicinal and therapeutic value.

Unfortunately, Indian fish farmers are unable to culture murrels due to non-availability of seeds

and lack of knowledge of seed production. They depend on fishermen for seed collection from

the wild which is unpredictable due to monsoon failure. Moreover, supply of seeds by fishermen

is only during southwest monsoon (June to August) and northeast monsoon (October to

December). Hence, induced breeding technique is necessary, as a boon to fish farmers for

availability of seeds throughout the year.

Research team of CARE (SXC, Tamil Nadu), the lead consortium, succeeded in induced

breeding of striped murrel (Channa striatus), spotted murrel (C. punctatus), dwarf snakehead

(C. gachua) and redline snakehead (C. diplogramme) using natural (HCG, pituitary hormone)

and synthetic hormones (LHRH, Ovaprim, Ovatide, Ova FH) by intramuscular injection to

brooders (each set: one male and two females). Two trials using fibre tanks of different capacity

in triplicates were made to observe the effects of different doses of HCG on induced spawning of

C. striatus. Fish which received a dosage of 6000 IU/ Kg body weight showed ovulation after

19- 29 h post injection. Fertilization rate was observed as 40-80% and hatching rate varied from

55- 80% which occurred within 22 – 36 h at water temperature of 27 - 29º C. The overall

breeding performance and the successful development of protocols for captive breeding is likely

to pave the way towards commercialization of the technology for upscaling of murrel seeds in

stakeholders farms.

The CIFA, Bhubaneswar, the partner institute, had developed spontaneous breeding

protocols for murrels (C. striatus) by creating natural ecological environment in pond. The eggs

were laid in the nests made by the fish biting the plants. These eggs and hatchlings were

collected and reared in hatchery. It was observed that about 20-30% of brood stocks responded in

naturally created environment. Each batch consisted of nearly 3000-4500 eggs or hatchlings. The

hatching time varied from 30-50 hrs depending upon temperature. The size of the hatchlings

ranged between 4.2- 4.4 mm.

In another study, following HCG injections (2000 – 6000 IU), after 6 hr, C. striatus became

active and frequently left the bottom swimming in the water column. Males arrived at activity

peaks 11 – 13 h following HCG injections, 2 – 4 h ahead of the females (14- 16 h). The

important observation was that the male was more actively involved in the courtship and

spawning. The male was hitting the snout and vent region of the female more frequently.

Courtship behavior indicative of spawning such as pairing, chasing and touching bodies was

observed. Brooders were found to “cruise together” making X shaped appearance (which is a

common behavior of edible murrels) in water column or frequently come together prior to

releasing eggs or milt. At the time of courtship, the male starts to tilt its body close to genital

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papilla of female and the breeders join together which ultimately resulted in spawning after about

26± 4 h duration followed by external fertilization. The fertilized eggs were yellow in colour

which usually floated and adhered to each other forming an egg mass of 10 – 15 cm in diameter,

whereas the unfertilized eggs were white in colour, non-adherent and were scattered throughout

the tank.

Aggressive behavior was shown by male parent who remained curving around the eggs and

showed circular movement fanning the eggs with its pectoral fins. After 24 h, fibre tanks were

found full of hatchlings black in colour. Both parents showed care for hatchlings by guarding

them right from the stage of fertilized egg till the fry stage. Though C. striatus showed biparental

care, it was observed that the male parent was more vigilant towards their young ones. It was

noted that the eggs guarded by the parents remained clean and showed good development and

reached post larval stage with high survival rate suggesting the importance of parental care to

increase the hatching rate and protection of eggs from fungal infection and predation.

Fecundity of 8500 – 28500 eggs were obtained from C. striatus weighing 550 - 1150 gm

(length 32.02 – 50.8 cm) from fish collected from Thamirabarani river (8º 42‟ N; 7º 24‟ E). The

highest Gonado Somatic Index (GSI) of 3.53 was noticed in fish weighing 780 g and low GSI of

2.50 in fish with 560 g. However, large sized fish (1150 g) showed low GSI (2.72) whereas the

less weighed fish (780 g) showed a high GSI of 3.53. A positive relationship was observed

between the fecundity and body length, body weight, gonad length and weight.

Based on the studies conducted by CARE research team, HCG was recommended to

stakeholders for upscaling of murrel seed production in their ponds. From a single breeding set

of striped murrel injected with HCG (2000 IU / Kg) produced 8,000 – 12,000 eggs. The year

wise seed production details are as follows:

Table 3: Year wise Seed Production of CARE and CIFA

Years SXC, Tamil

Nadu

At CIFA,

Bhubaneswar

By Stakeholders Total

2009-10 11,361 41,000 - 52,361

2010-11 36,500 37,820 22,500 96,820

2011-12 60,028 46,938 35,000 1,41,966

2012-13 NA 75,000 15,000 90,000

2013-14 NA 62,500 37,500 1,00,000

Total 1,07889 2,63,258 1,10,000 4,81,147

*NA- No Seed Production activity for SXC, the Lead Institute during Extension Period

Expected Impact of the project:

For production of 1 lakh seeds - Brood fish maintenance pond (0.10 acre), Breeding pond

(0.25 acre), Nursery tanks (0.50 acre), Fry rearing tanks (0.65 acre), a land of 1.5 acres is

recommended.

For small scale production of 10,000 fingerlings, 8-9 hatcheries (each 3ft diameter with 3 ft

depth) are necessary requiring a total area of 289 sq ft. The expenditure involves Brood

Fish, Feed, Hormone, Plankton Culture Tanks, Hatcheries, etc and the net profit is

Rs.65,000/- per year for the production of 10,000 fingerlings in an area of 289 sq.ft.

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2. Mass culture of Live feed

Responsible Partner: TNC, Chennai - Partner Institute

The eggs of striped murrel C. striatus hatched out within 24 h and the hatchlings started

taking exogenous feed from third day onwards. The growth and survival of hatchlings depend

much on the type of feed and hence live feed culture occupies an important role in murrel

farming to decide the success of farmers. Live feed comprise phytoplankton, zooplankton,

earthworm, annelids and molluscs. Provision of suitable live feed is the bottleneck in rearing

larvae of C. striatus. Rotifers were found to be an excellent feed for rearing post larvae and fry

especially during early stages, while the Cladocerans, Moina and Daphnia were recommended

for the advanced fry and fingerlings.

As the cladocerans are considered to be suitable live feed

for fish larvae, for finding a cheap and suitable medium for

culture of Ceriodaphnia cornuta studies were carried out for 21

days using chicken manure whereupon C. cornuta population

ranged between 50±2 and 10,232±202 Ind/L (Individuals/litre).

The culture peaked on the 17th

day producing the maximum

density of 10,232± 202 Ind/L suggesting chicken manure as a

suitable medium at 700 ppm for mass culture of cladocerans.

The branchipod Moina micrura was mass cultured using

three different concentrations (250 ppm, 500 ppm and 1000 ppm)

of chicken manure in the medium for 21 days. The maximum

density of 1694.44± 9.68 was obtained on the 9th

day of the

inoculation at the concentration of 250 ppm and the number of

plankton declined gradually afterwards. In 500 ppm

concentration, the maximum density (7296.88 ± 54.35) appeared

on the 11th

day while in the third concentration, maximum

density (1510 ± 15.27) was observed on the 14th

day of

inoculation. Young Moina are less than 400µm in size,

approximately of the same size of the adult rotifers.

CARE and TNC research team also succeeded in

Mass culture of Zooplankton,

Culture of cladocerans and copepods (Ceriodaphnia cornuta, Moina micrura and Daphnia

carinata and Thermocyclops decipiens) and density of zooplankton /litre was reported for

Cladocerans (8,000/l), Copepods – (7,000/l), Rotifers (10,000/l) in fiber tanks of 10,000 litres

capacity.

3. Larviculture Technology

Responsible Partner: CARE, St.Xavier‟s College, Lead Institute

Rearing of murrels from the post larvae to the fingerling stage needs extra care and is a

Herculean task. Three days old post larvae were fed on rotifers for 15 days. Apart from

zooplankton, the larvae were also fed on other live feed organisms like blood worms

(Chironomous larvae), tubifex and earthworms. Boiled chicken intestine paste and fish paste

were supplied as feed for the larvae from the 16th

day onwards instead of live feed. From 30th

day

Fig 4 Population density of D. carinata during

different days of culture at different feeding

rates (Mean ± S.E.)

Fig 3 Population density of Ceriodaphnia

cornuta during different days of culture at

different feeding rates (Mean ± S.E.)

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onwards, the fry were fed with cooked and minced chicken intestine and semi-moist pellets and

were later trained on pelleted feed (semimoist) during fingerling stage.

Dietary herbal additive Phyllanthus niruri, resulted better growth performance and

immunostimulation in C. striatus fingerlings. All growth and hematological parameters increased

in response to supplementary diet P. niruri. In P. niruri fed group, the mean weight gain (5.166

g) and specific growth rate (3.63) were the highest when compared to the control group (2.915).

Incorporation of P. niruri diet increased haemoglobin (Hb) (26.85%), red blood cell count (43

±0.38), white blood cell count (29 ±0.25) and mean corpuscular haemoglobin concentration

(33.23g/dl). haematocrit (36.41 ±4.2), mean corpuscular haemoglobin (2.81) and mean

corpuscular volume (8.46fl) also increased. P. niruri acted as a potent immunostimulant, by

inducing the blood parameters in C. striatus.

In the next study, diets were prepared using the feed additives viz: bacterium Bacillus

subtilis, Indian almond Terminalia catappa and aloe Aloe vera. C. striatus fingerlings fed on

control diet showed 2.881g weight gain, whereas those supplied with T. catappa diet gained

6.319g body weight followed by A. vera diet (2.915g) and B. subtilis diet (2.894g). The specific

growth rate (3.96) and condition factor (0.8) of T. catappa fed groups were higher than that of

other supplemented fed groups. During post A. hydrophila challenge, highest survival rate was

observed in Aloe fed group (100%) followed by B. subtilis (90%), T. catappa (90%) and control

(60%) fed groups. Survival of striped murrel fed diets supplemented with aloe was the greatest

post challenge with A. hydrophila. Survival of striped murrel fed diets supplemented with

B. subtilis and Indian almond was greater than that of fish fed on control diet. The improved

survival suggests that production of metabolites by probiotics suppressed the pathogenicity of

A. hydrophila and ultimately resulted in better survival and growth of the host.

The cellulolytic, amylolytic and proteolytic activities of bacteria were isolated and

enumerated from the gastrointestinal tract of murrel (C. striatus) fingerlings (22±0.43g) fed with

herbals like Murraya Koenjii, Sesbania grandiflora and Amaranthus thandu (I – III), probiotics

like B. coagulans, B. subtilis and mixed probiotics (IV – VI) and chicken intestine diet (VII)

with group VIII as control. At the end of culture, the maximum average growth of group – IV

fish was higher (3.51±0.20) than that of the control – VIII (2.01±0.20). At the end of

Fig 5 Weight gain (gm) of C. striatus

fingerlings fed on P. niruri diet

Fig 6 Effect of feed additives on weight gain in C.

striatus fingerlings fed different feeds

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experimental period, the survival in group VII was 98% whereas in control, it was 90%. The

cellulolytic acitivity was higher in group-IV fishes (93.2±0.81Umg-1

), than control group

(5.9±0.84Umg-1). The amylolytic and the proteolytic activities were higher i.e., 3.3±0.53Umg

-1

and 53.4±0.31 Umg-1

in group III respectively and the differences were statistically significantly

(p<0.05). The results of the present study showed that the chicken intestine and B. subtilis have

better impact on murrel culture

Responsible Partner: TNC, Chennai - Partner Institute

Culture performance of larval snakehead (Channa striatus) was examined by feeding them

with cladocerans (Ceriodaphnia cornata, Miona micrura and Daphnia carinata), and Artemia

nauplii as individual and mixed diets for 28 days. Fish fed Artemia nauplii, C. cornata and

mixed cladocerans showed higher weight gain (15.88± 0.11 mg), (9.72± 0.04 mg) and (10.0 ±

0.06 mg) respectively during the first week, whereas fish fed C. cornata and M. micrura

showed better weight gain (12.88± 0.21 mg) and (11.90±0.09 mg) respectively during the

second week. Less cannibalism was observed in fishes fed with mixed cladocerans in the last

two weeks. Use of cladocerans for early larval rearing of C. striatus will reduce the

expenditures met on seed production, as the nauplii of brine shrimp Artemia extensively used in

hatcheries are exotic and highly priced resulting in

higher cost of seed production. It was noticed that

cannibalism was reduced at different stages by

providing prey of suitable size to the growing fish.

Feeding trials were conducted for rearing C.

striatus fry using different feeding regimes for 30

days viz: fish paste, chicken intestine paste,

chopped prawn, cladocerans, cladocerans with

chicken paste and chopped prawn with cladocerans.

Fishes were fed ad libitum twice a day. Significantly high survival rate (90%) and better weight

gain (354.55± 43.79%) were observed in the fry fed on chopped prawn with cladocerans

followed by those fed on cladocerans with chicken intestine paste (336.88± 44.25%). High

mortality was recorded in fry fed with fish paste and chicken intestine paste without

cladocerans. Hence, the importance of live feed in the survival and growth of murrel fry is

inevitable.

Feeding experiments were conducted for the postlarvae of C. staritus with two different

live feeds - copepods (Thermocyclops decipiens) and cladocerans (C. cornuta and M. micrura)

individually and in mixture at the rate of 500±50 Ind./L for 30 days. Results indicated better

weight gain (951.85±28.77%) and survival (92%) of postlarvae fed with mixed live food than

individual live feed organisms.

Fry and fingerlings were fed with four types of feed viz., chicken intestine, fish waste,

chicken intestine & fish waste together (50:50) and zooplankton (T. decipiens, C. cornuta & M.

micrura ) at 5% BW to examine their effects on survival and growth for 30 days. Better weight

gain of 115.32 ±1.35g for chicken intestine feed in case of fingerlings and 253±1.67 g for live

Fig 7 Weight gain in C. striatus larvae fed different feeds

in different weeks

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feed in case of fry were observed suggesting the preference of live feed by fry and chicken

intestine by fingerlings over other feeds.

Responsible Partner: CIFA, Bhubaneswar, Partner Institute

The low survival is a major problem in larval rearing of murrels. Feeding started 72 h after

hatching with micro zooplanktons especially rotifers. Nursery rearing has always been preferred

in concrete tank for high recovery and better management of this species. Size heterogeneity and

cannibalism are prominent during seed rearing. Hence periodic segregation and separate rearing

of same size group is necessary to achieve good survival. About 2-3% of the hatchlings turns

into shooters and take heavy toll on smaller ones. Survival in the nursery (spawn to fry) ranges

from 50-60% over a period of 20-25 days.

Fingerling rearing is carried out in concrete tanks with live feed (insects, tubifex,

earthworms and small prawns); they readily accepted boiled chicken viscera/trash fish: rice bran

(3:1) as supplementary feed preferably in dough form. Protein requirement for fingerlings was

about 40-42 %. Survival from fry to fingerlings is 40-50%. Furthermore, during the seed rearing

either from larvae to

fry or fry to

fingerling, periodic

removal of bigger

ones is an essential

process to avoid

cannabalism to get

high survival.

Therefore, it is

necessary to net the

tank at weekly

interval and check

them for shoot fry / fingerling and culture them separately.

4. Pelleted Feed Technology

Responsible Partner: CARE, St.Xavier‟s College, Lead Institute

There is no readymade feed for murrels in market, since they are carnivorous, piscivorous

and cannibalistic. Formulated diet containing fish meal (anchovy and jawala) 60%, wheat flour

10%, rice flour 10% and soya flour 20% respectively with 43% of protein was prepared for

murrels.Feed pellets prepared using pelletizer ranged between 2.5 -5.0 mm were readily accepted

by murrel fingerlings.

Antibiotics play an important role in the disease management of aquaculture practices;

however antibiotic resistance develops readily in pathogens following antibiotic treatment.

Medicinal herbs play an alternative role to tackle this problem. To enhance the survival and

growth and to improve the health of the fingerlings, the pellets have been incorporated with

herbs (Phyllanthus niruri, Aloe vera, Terminalia catappa, etc) and probiotics (Bacillus subtilis,

Saccharomyces cervisiae, Lactobacillus helveticus, L. acidophilus, L. plantarum, L. sporogens,

Candida utilis etc).

Fig 8 Survival

of

murrel

seed during

different

stages of seed

rearing

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Antimicrobial activity of methanolic extracts of eight medicinal plants (Ocimum sanctum,

Psidium guajava, punica granatum, Tinospora cardifolia, Withania sominifera, Allium sativum,

Terminalia arjuna and Mentha arvensis) against the common fish pathogen Aeromonas

hydrophila. Among the eight medicinal plant extracts T. cardifolia (0.780mg/ml) and W.

sominifera (1.560mg/ml) showed a wider spectrum of antibacterial activity and least MIC and

MBC values against A. hydrophila. All the other herbal extracts showed a moderate antibacterial

activity against the test pathogen. The MIC values for T.cardifolia and W.sominifera are

estimated as 0.780mg/ml and 1.560mg/ml respectively and were suggested for treating diseases

caused by A. hydrophila in aquaculture practices. Leaf powder of T. cardifolia (5 g/Kg feed) and

root powder of W. sominifera incorporated in fish feed (25g/Kg feed) resulted in cent percent

survival of C. striatus fingerlings.

In another study, fifteen selected plant species belonging to Lamiaceae and Apocynaceae

were evaluated and against the fish pathogen, Aeromonas hydrophila. The antagonistic effect of

the methanolic extracts of Coleus aromaticus of Lamiaceae and Tabernaemontana divaricata of

Apocynaceae were found to be most effective against A. hydrophila. Regarding Lamiaceae,

C. aromaticus, Mentha arvensis and Leucas aspera exhibited lower MIC value of 12.5 mg/ml

with inhibition zones of 10.33 mm, 9.67 mm and 9.33 mm respectively. T. divaricata of

Apocynaceae showed MIC of 12.5 mg/ml with an inhibition zone of 7.33 mm whereas

Catharanthus roseus at 25 mg/ml exhibited an inhibition zone of 9.67mm and Rauvolfia

tetraphylla at 50mg/ml showed an inhibition zone of 9.33mm. Considering overall performance,

C. aromaticus was found to be the most effective antagonistic agent against A. hydrophila.

5. Growout Culture

Responsible Partner: CARE, St.Xavier‟s College, Lead Institute

Earthen ponds ranging from 0.1 ha to 0.4 ha with a depth of 1m with a stocking density of

1/m2

were suggested suitable for murrel culture. Before stocking, the pond was fertilized using

organic manure, viz. cow-dung and poultry waste. Water quality parameters may not be a serious

factor, since murrels are air breathing and survive in oxygen depleted water bodies. For

commercial murrel culture, earthen ponds of various dimensions (depending on land availability)

could be recommended. Once the fingerlings were trained for feeding on semi- moist or pelleted

feed (consisting of fish meal, soy flour, wheat flour, rice flour and tapioca), they were introduced

into the culture ponds. At present, murrel farmers supply the feed as dough in trays and gunny

bags. ICAR-NAIP Research team has trained murrel fingerlings to take pelleted feed at CARE

Aquafarm and stakeholders farms. Usually the culture period is about 8 - 10 months to obtain

table size fish of 750g weight. It is better to harvest before one year to avoid excess cost of feed.

Under the subproject, 52 farmers practiced murrel culture in Tamil Nadu and they harvested

murrels in a total area of 266.56 ha with a production of 27.67 tons in Tamil Nadu. Murrel

farmers harvested 100 Kg table size murrel/ha. The market price of table size murrel varied from

Rs.280/- from the inception of the project to Rs.700/- by 2013 depending upon the area, water

availability and demand for murrels.

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Table 4 Year wise Table size Murrel Production in Tamil Nadu

Year No. of

farmers

harvested

Total

Area

(ha)

Harvested

Murrels

(tons)

Income

Generated

(Rs.)

Production

in tons/ ha/

yr

Income

generated

per ha/ yr

2010-2011 19 250 17.34 33,51,450/- 0.069 13,405/-

2011-2012 20 7.74 5.73 16,13,000 0.740 2,08,398/-

2012-2013 13 7.92 4.6 13,26,200 0.580 1,67,450/-

The length-weight relationship of captured (wild) and cultured C. striatus was compared

using statistical tools. Significant (p<0.05) variations were recorded when the length and weight

of wild Channa striatus collected from Nagercoil was compared with cultured C. striatus of

CARE, Araikulam, Kaliyaoor, and Veeralaperunselvi. The length and weight of Nagercoil wild

C. striatus was 40.36 cm and 653.5 g which significantly (p<0.05) varied from the length and

weight of CARE (32.9 cm and 501.16 gm), Araikulam (38.03cm and 583.50 gm), Kaliyaoor

(35.56 cm and 410.05 gm) and Veeralaperunselvi (39.13 cm and 579.50 gm) samples. Similarly,

the Nagercoil wild C. striatus significantly (p<0.05) differed from wild C. striatus of

Melapalayam. The variations are represented graphically as Y and R2

values in fig. 9 – 12

indicating that the captured wild striped murrels gained more weight than those of the cultured

murrels.

Fig 9 Length – Weight relationship of wild C. striatus

purchased from Nagercoil fish Market

Fig 10 Length – Weight relationship of wild C.

striatus purchased from Melapalayam fish Market

Fig 11 Length – Weight relationship of C. striatus

cultured at CARE, Palayamkottai

Fig 12 Length – Weight relationship of C. striatus

cultured at Arasan Aquafarm,

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The length and weight of four wild species of C. striatus from Melapalayam were 43.3

cm and 651.5 g which significantly (p<0.05) varied from the length and weight of CARE (32.9

cm and 501.16 gm), Araikulam (38.03cm and 583.50 gm), Kaliyavoor (35.56 cm and 410.05

gm) and Veeralaperunselvi (39.13 cm and 579.50 gm) cultured individuals. Similarly the

Melapalayam wild C. striatus significantly (p<0.05) differed when compared with wild

C. striatus of Nagercoil (df = 29; F = 2.130; p = 0.036). Both captured samples (Nagercoil and

Melapalayam) and one cultured sample (Arasan Aqua Farm) were positively allometric in their

growth (Fig.9, 10 and 12), while C. striatus cultured at CARE was negatively allometric

(Fig. 11). The respective exponential equations are: wild samples collected from Nagercoil

(WT=14.34L+26.50), Melapalayam (WT=14.59 L+20.39), and C. striatus cultured at CARE

Aquafarm (WT=7.966L+5.499) and Arasan Aquafarm (WT=7.966L+5.499) are given in Fig. 11

and 12. Graphical representation of the condition factors of wild and cultured C. striatus is

shown in Fig. 13.

Responsible Partner: CIFA, Bhubaneswar, Partner Institute

CIFA, Bhubaneswar took the challenge and standardized the growout farming

technology of murrels under the NAIP project. The brief description of growout culture of

murrels technology is as follows. Ponds with steep dyke and size-0.1-0.5 ha are preferably

used. The recommended stocking density for grow-out under monoculture is 10,000

fingerlings/ha of the size 10-20 g. Fishes are fed boiled poultry viscera/trash fish mixed with

rice bran (7:3). It is also advisable to promote inherent food production in the pond such as

insects, weed fishes etc. for better production. The feeding rate of 2-5% of their body weight is

given and it is gradually reduced as the growth advances. Murrel (C. striatus) grows to 0.6-1.0

Kg in culture period of 8-10 months. For total harvesting, dewatering of pond is required and

fishes are hand-picked. The average production level of 2-2.5 tons/ha/yr has been

demonstrated. There is a great potential for this species to be taken up with carp polyculture

system.

Dissemination of murrel growout culture to farmers of Orissa

According to available information and our baseline survey, it was evident that there was

no scientific murrel culture practices followed in Orissa during pre-project period. In Orissa,

there is a social taboo for consumption of giant murrel and also standard technology for breeding

Fig 13 Condition factor for wild and cultured

C. striatus

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and its culture were not available. These reasons made them skeptical to start murrel culture.

There is a traditional practice to catch murrels from rice fields or derelict water bodies in small

quantities for consumption. Since most of the murrel production coming from common

resources, it is difficult to estimate the exact contribution to the individual farmers‟ income.

Murrel farming offers tremendous potentialities for providing employment opportunities

to the rural people including women. In rural areas, most of the small backyard, shallow,

community and weed infested ponds are not used for fish culture. These water bodies are suitable

for the murrel culture. Since, it is new technology CIFA conducted number of training and

workshops under NAIP project “A value chain on murrel production in Tamil Nadu and Orissa”

about seed raising, feeding and culture of murrels for the SHGs (especially women), NGOs and

private farmers of Odisha in vernacular language to educate them for murrel culture. Over 300

farmers, including more than 180 women were given training on various aspects of murrel

farming. Institute also took help of print media (English & vernacular language) for making

people aware of murrel culture. Due to our concerted effort, people are now realizing the

potential of murrel culture and many of them have come forward to take up this technology.

Murrel farming has been demonstrated in Puri, Khurda and Nayagadh Districts, Odisha. The

CIFA has demonstrated grow out culture technology in farmers‟ ponds in selected places of

Orissa state with average production level of 2.0-2.5t/ha/yr. The additional income generation

from murrel farming not only improved their livelihood but also helped in nutritional security.

After seeing the success many more farmers are coming forward to start murrel culture. The

scientific murrel farming started from scratch in 2009 and at present about 20.0 acres of water

area is under its farming.

CIFA under the NAIP- ICAR project “A value chain on murrel production in Tamil Nadu

and Orissa” made a baseline survey to know the resources and interest of fish farmers towards

culture of murrels. A number of training and workshop were provided to the SHGs, NGOs and

private farmers in vernacular language to educate them for murrel culture. Based on their interest

and available resources four different groups i.e., 1 woman SHG (T1), 1 man SHG (T2), 1 NGO

(T3) and 1 private fish farmers (T4) were selected. Pond sizes were in the range of 0.02-0.6 ha.

The standard pond management practices were followed where they were advised not to

eradicate small trash fishes. The ponds of different groups were stocked with advanced

fingerlings (avg. wt. 17.21 g) of C. striatus @ 5,000/ha. Fishes were fed with boiled poultry

viscera/trash fish (80%) with rice bran (10%) and oil cake (10%) @ 3-5% of their body mass

daily. After 8 -10 months of culture, fishes (0.5 - 1.0 Kg) were harvested by dewatering the

ponds. The highest production was observed in T3 group (2583 Kg/ha/yr.) and lowest in T1

group (1895 Kg/ha/yr.). The highest production in T3 might be due to their efficient management

and addition of live trash fish in the pond. This indicates that supplementation of small trash fish

is better for growth of murrels. The additional income generation from murrel farming not only

improved their livelihood but also helped in nutritional security. After seeing the success many

more farmers are coming forward to start murrel culture.

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6. Murrel Transport:

Responsible Partner: CARE, St.Xavier‟s College, Lead Institute

Fish vendors meet heavy loss due to death of murrels during transport. Sedation or use of

anesthetics is beneficial during bulk transportation. All the fish exposed to different

concentrations of clove oil recovered well and returned to normal after 48 hrs. For 48 hrs

transport a dose of 450 ppm of clove oil was recommended to fish vendors to anesthetize

C. striatus. Clove oil was used as fish anesthetic to study the hematological and biochemical

changes in C.striatus. Erythrocyte count (T/L), Hemoglobin and Hematocrit values of clove oil

treated fish showed elevated levels when compared to control. RBC, Hb and Ht values

significantly increased 1 hr after anesthesia and returned to normal after 24 hrs. Anesthetic

treated fishes exhibited marked

decrease in WBCs when

compared to control group. The

anesthetic treated fishes were

found to show a significant

increase in the concentration of

glucose. Results of the study

suggested that the use of clove

oil at the concentrations of 400,

450 and 500 ppm does not

cause irreversible damage on

the blood parameters as well as biochemical profile in C. striatus.

7. Murrel Disease Management

Responsible Partner: CARE, St.Xavier‟s College, Lead Institute

The crude extracts of a medicinal herb viz; Wrightia tinctoria was successfully used to

prevent EUS occurrence and enhance survive during larviculture and growout culture. Leaves of

W. tinctoria applied as paste on the lesion and ulcers externally cured EUS within four to six

days. The antibacterial and antifungal activity of W. tinctoria was studied against pathogens

Pseudomonas fluorescens, Aeromonas hydrophila, Staphylococcus epidermidis, Aeromonas

salmonicida, Aphanomyces invadans and Vibrio alginolyticus. Phytochemical screening of the

leaves revealed the presence of steroids, reducing sugars, alkaloids, phenolic compounds,

Time in

minutes

Concentration in ppm

Fig 14 Murrel production in the ponds of different stakeholders

Fig 15 Effect of Clove oil on Induction and Recovery time of C. striatus

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flavonoids, saponins and tannins. The TLC chromatogram of the extracts revealed several

coloured bands. The methanol extracts showed efficient antibacterial activity15.6 ±0.3/67.1

(Inhibition zone diameter in mm/RIZD in %) against A. hydrophila. The minimum Inhibitory

concentration of the extract against the pathogens was determined. Growth of A. invadans was

inhibited by 85-90%. The herbal paste applied topically on the lesions showed a positive effect

by controlling and curing the lesions within five days. Following 2nd and 3rd

day post application

of herbal paste, healing symptoms were noticed and signs of dermal lesions and ulcers vanished

and the wound was completely healed by the 5th

day. No mortality was observed during the

course of the study. It is therefore suggested that the active extract of W. tinctoria could be taken

to the next step of bioassay guided purification to characterize the novel antimicrobial agents.

In another consecutive study, the medicinal plant Coleus aromaticus was used in treating

EUS infected giant murrels C. marulius showing symptoms like lesions and deformed fins at the

dorsal side with reddish spots on the ventral side. The herbal paste was applied topically on the

wound surfaces of diseased murrels for 10 days. From 5th

day onwards, the treated murrels

showed good signs of healing like disappearance of lesions and reddish spots and recovery of

affected fins, thus recommending the topical application of herbs for effective treatment of

diseased murrels.

Responsible Partner: CIFA, Bhubaneswar, Partner Institute

A preliminary study was conducted during winter months to know the effects of plant crude

extracts on growth, survival and health status of C. striatus fingerlings in cemented cisterns of

capacity 1000 L in field condition. The herbal crude extract was prepared separately from the

leaves of Ocimum sanctum (Tulsi), Aegel marmelos (stone apple) and rhizome of Curcuma longa

(turmeric) and kept in sealed poly packs under refrigeration. C. striatus fingerlings (6.15 + 0.2g)

were fed on murrel feed containing different medicinal plant extracts for 60 days. The control

group was fed same feed but without herbal extract. Fishes were observed fortnightly for growth,

survivability, gross health examination and bacterial total plate count of whole content of

intestine and gills.

Fishes were fed feed containing turmeric showed significantly higher (p<0.05) survival

(93.33 %) in comparison to control (83.33%) and other herbal crude extract fed groups. There

was no significant difference in growth between control group and crude extract fed groups.

Gross morphological examination showed that fishes fed herbal crude extracts were healthy

(shiny body, intact fins, active movement, etc) in comparison to the control group. The microbial

studies revealed that O. sanctum fed fishes contains significantly less (p<0.05) number of

bacteria in gut i.e., total plate count (2.35 x 107

cfu) in comparison to fishes of other herbal crude

extract fed or control (3.40 x 109

) groups. Similar result was observed for gills. The study

suggests that the herbal crude extracts could be used for prophylaxis to keep fishes healthy and

also to enhance their survival during fingerling stages.

Table 5 Effect of herbal paste on growth and survival of C. striatus fingerlings

Treatments Initial mean

weight (g)

Final mean

weight (g)

Growth (%) Survival (%)

C 6.15 + 0.2 31.78 + 0.40 416.7 + 06.44 83.33a + 0.00

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T1 6.15 + 0.2 31.17 + 0.86 406.8 + 14.02 86.66ab

+ 4.70

T2 6.15 + 0.2 30.92 + 1.25 390.2 + 39.08 93.33c + 0.00

T3 6.15 + 0.2 30.15 + 2.40 402.8 + 20.35 90.00bbc

+ 4.71

The incidence of Ichthyophthirius multifilis was reported by CIFA, Odisha during the

nursery rearing of striped snakehead. This was successfully controlled with continuous

application of 10 ppt formalin for 5 days. This helped in total eradication of parasites from

infected seed and finally mortality was checked completely.

Acinetobacter baumannii acts as a severe human pathogen, there are only few studies to

date that report it as a pathogen for fish. In the present study, one virulent bacterial strain was

isolated from diseased C. striatus, from a farm at the CIFA which showed symptoms like cloudy

eyes, pop eye (exophthalmia), Opaque lenses and mild ulceration on the whole body irrespective

of sex and size of the fish. Based on morphology, different biochemical tests and sequence

analysis of 16S rDNA segment as well as phylogenetic study, the causative bacterium (called

chE) was identified as A. baumannii. The pathogenicity was further confirmed by artificial

infectivity study (LD50 dose of 108.37

CFU/fish). In the drug sensitivity study, this isolate was

highly resistant to many antibiotics. The isolate was also highly resistant to all three tested heavy

metals (Cu+2

, Cr+6

, Hg+2

), thus proving its virulent nature. The involvement of A. baumannii in

disease of Channa species poses a potential threat to freshwater fish farming as an important

emerging pathogen. Further, the resistance to many antibiotics and heavy metals shown by this

isolate poses serious concern.

8. Medicinal value of murrels:

Responsible Partner: CARE, St.Xavier‟s College, Lead Institute

a) Antibacterial activity of muscle extracts

Five crude extracts from the muscle tissues of four Channa species namely, Channa striatus,

C. punctatus, C. marulius and C. diplogramme were screened against five enteric pathogens

namely Salmonella typhi, Staphylococcus aureus, Klebsiella vulgaris, Shigella dysenteriae and

Shigella boydii. Almost all the five extracts had efficient antibacterial activity against the

pathogens. The zone of inhibition ranged from 24.6 to 7.0 mm. The methanol extract of C.

striatus showed the highest inhibition zone of 24.6, 21.6, 18.0 mm against S. dysenteriae, K.

vulgaris, S. typhi respectively and C. marulius showed 23.2, 22.1 mm against S. boydii, S.

aureus. In all the species the inhibition observed was in the order of methanol> chloroform>

acetone> benzene> water against the bacteria. Water extracts of all the species showed least

activity against the pathogens. . The tissues of the Channa species which showed the efficient

antibacterial activity were subjected to SDS –PAGE to study the molecular weights of the tissue

protein. Crude protein sample of four Channa species yielded 34 bands ranging from 16.64-

232.76 k Da. The highest number of band was found in C. diplogramme and the least number of

bands was found in C. punctatus. Efficient antibacterial activity was observed from the methanol

extract of all Channa species. A maximum zone of inhibition was observed in C. striatus against

Shigella dysenteriae. It is therefore suggested that C. striatus can be a potential source of an

antimicrobial protein for specific human pathogens

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26

.

B1 - Salmonella typhi, B2 - Staphylococcus aureus, B3 - Klebsiella vulgaris, B4 – Shigella

dysenteriaand B5 – Shigella boydii; A – Acetone, B – Benzene, C – Chloroform, M – methanol

and W – Water

b) Antibacterial activity of skin and intestinal mucus

The antibacterial activity of skin mucus and intestinal mucus of five different Channa sp viz

Channa striatus, C. micropeltes, C. marulius, C. punctatus and C. gachua was tested against five

pathogenic bacteria namely A. hydrophila, P. aeruginosa, E. coli, Vibrio fischeri and V.

anguillarum using Muller Hinton agar plates by disc diffusion method. All the five Channa sp

showed a better antibacterial activity and among the five species C. straitus showed a broad

spectrum of antibacterial activity of skin mucus against A. hydrophila (19.5±2mm), P.

aeruginosa (28±2.9mm) and V. anguillarum (24.5±2.4 mm) and intestinal mucus against A.

hydrophila (16±1.4 mm), P. aeruginosa (29±3.2 mm), V. anguillarum (18.3±1.5mm) and V.

fischeri (21.5±1.8). Among the five pathogens tested, the maximum antibacterial activity was

observed for the skin mucus against V. fischeri and the intestinal mucus against P. aeruginosa,

suggesting the presence of antimicrobial peptides /proteins in fish mucus that makes the

membrane to act as barrier.

c) Fatty acid composition

The fatty acids present in the murrel fillet were studied. The prevailing compounds in murrel

fillet were found as 9-Octadecenoic acid (Z)-, methyl ester (56.69%), Hexadecanoic acid, methyl

ester (21.02%), Octadecanoic acid, methyl ester (10.74%), Arachidonic acid methyl ester

(1.87%) and 9-Hexadecenoic acid, methyl ester, (Z) (2.84%). The other compounds include

Dodecanoic acid, methyl ester (0.82%), Tetradecanoic acid (0.64%), 9,12,15-Octadecatrienoic

acid, Methyl Ester, (Z,Z,Z)(0.56%), Cyclopropaneoctanoic acid, 2-hexyl-, methyl ester(0.68%),

Eicosanoic acid, methyl ester (0.28%) and 8,11,14-Eicosatrienoic acid, (Z,Z,Z)(0.61%).

d) Wound healing studies:

Wound healing studies on male wistar rats using murrel extract revealed faster healing upon

topical application of C. striatus crude extract but the activity was maximal at the high dose (400

mg/Kg p.o) applied. The wound was healed completely (100%) by 21st day in 10% concentration

whereas in the same 100% healing was observed on 18th

day for standard treatment. The PUFA/

Fig 16 Antibacterial activity of the tissue extract of Channa species

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27

Fig. 1: Net Fillet Yield of Murrels

Fillet

40%

Head

23%

Bone

7%

Skin

8%

Viscera

22%

Saturated (P/S) ratio of C. striatus fish oil is 0.69 which is higher than the Patin (Pangasius

hypothalamus) with 0.67. On the other hand, the ω-3/ ω-6 ratio of C. striatus is 0.96 which is

higher than the catfish, Clarias macrocephalus (0.25) and Patin (0.73).

e) Anti-inflammatory studies:

The striped murrel extract at 400mg/kg dose showed significant decrease in the paw volume

(65.59%) of wistar rats when compared to the control group. C. striatus at the dose of 400

mg/Kg showed, potent anti-inflammatory activity when compared with the reference standard

Indomethacin.

f) Anti-arthritis studies:

Arthritis study was done by inducing arthritis in male wistar rats by injecting Freund‟s

complete adjuvant (FCA). C. marulius (high dose) rendered about 91.72% healing whereas C.

striatus (high dose) crude extract rendered 88.43% healing. The Standard drug yielded 92.86% in

the male wistar rats. The low dose (200mg/Kg p.o.) of C. marulius rendered about 89% healing

whereas C. striatus crude extract rendered 84.31% healing. In both the low and high doses, C.

marulius rendered more protection than C. striatus.

9. Murrel Filleting Technology

Responsible Partner: CARE, St.Xavier‟s College, Tamil Nadu Lead Institute

All over India, murrels are sold as live. Dead murrels are not preferred by consumers and

hence fish vendors quite often meet heavy loss. At this juncture, filleting technology, i.e.,

boneless preparation of murrels is the opt one to meet the need. This technology is known to fish

vendors and consumers in places like Gazhipur market, New Delhi, but it is not spread to

markets of other states especially Tamil Nadu, Kerala and Karnataka. For the first time, the

research team of ICAR-NAIP Sub project succeeded in preparation of fillet from live as well as

frozen murrel. Though murrel fillet has not gained much attention among consumers, CARE

research team is popularizing this technology among housewives, working women, chefs of star

hotels and restaurants, who can adopt this processing technology to prepare several fast food

items as less time consuming by conducting different Expos.

People live in Metropolitan cities viz: Chennai, Mumbai, Delhi and Kolkata and those live in

Cosmopolitan cities like Hyderabad, Bangalore and Cochin are exposed to Fish Food Festivals

like INFISH 2010 (Hyderabad), MATHSYA MELA 2011, RASHTRIYA KRISHI MELA 2011

(Bangalore). But those from southern districts of Tamil Nadu (Tirunelveli, Tuticorin and

Kanyakumari) are not aware of such programmes. Moreover, they are not familiar to the

importance of fish as healthy food. Hence, Fish food expos viz: Murrel Fish Food Expo 2012,

Nellai Fish Food Festival 2013 and Nanjil Fish Food

Festival 2013 conducted by CARE gave an opportunity

for them to attend training on seed production and

culture technologies and live fish recipe demonstration

by leading Chefs and selected women and competition

for essay writing and display of homemade fish food, to

witness live display of freshwater food fishes and value

added products, as a means of popularization of murrel

Fig 17 Net Fillet Yield of Murrels

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28

MURREL

FISH

MURREL FILLET MURREL SOLID

WASTE

MURREL LIQUID

WASTE

COMPLETE FOOD NUTRITIONAL

SUPPLEMENT

READY TO SERVE

PRODUCTS

READY TO COOK

PRODUCTS

MURREL PROTEIN

POWDER

NUTRITIONAL

VALUE

MEDICINAL

VALUE

SOUP POWDER MURREL MUCUS

MURREL SKIN

MANURE

Fig 18 VALUE ADDITION IN MURRELS – Diagrammatic Representation

fish production, value added products and processing techniques during 2012 and 2013.

Filleting technology yields 40% fillet, 23% head, 22% viscera, 8% skin and 7% bone and the

cost of fillet is Rs.800/Kg. The process of filleting involves various steps viz: stunning of fish,

grading, removal of slime, scaling, washing, deheading, gutting, cutting away the fins, slicing the

whole fish into steaks, skinning and meat-bone separation. For the removal of head, the fish is

held flat and the head is cut off with the help of a knife. Then, the entire dorsal ridge including

the fins is cut deeply upto half an inch. This is followed by degutting of internal organs like

gastrointestinal tract, heart and kidney and the peritoneal cavity is washed thoroughly. The

degutted murrel is then cut laterally into two equal halves so as to remove the skin easily. Then

deboning is done, with the fillets being obtained as a boneless flesh. The fillets can be preserved

at -20°C for 30 days. This technology was tested and fillet was used for preparation of more than

30 recipes by us (Please refer Annexure II).

i. Murrrel Value Addition

Value added products viz., Murrel Fry, Murrel Gravy, Murrel Briyani, Murrel Vermicelli,

Murrel Pollichathu, Murrel Cutlet, Murrel Pickle, Murrel Soup, Murrel Curry, Murrel Poori,

Murrel Finger chips, Murrel Papad, Murrel Noodles, Murrel Fish Soup Powder, Murrel

Shawarma, Murrel Gelatin, Murrel Spring Roll, Murrel Salad, Murrel Tikka, Crispy Murrel,

Murrel Ghatti rolls, Murrel Puffs, Southern Fried Murrel, Murrel Cookies, Murrel Dhall powder,

Muurel Bonda, Murrel Bajji, Murrel Fish Flakes, Murrel Vadai, Murrel Sticks and Murrel Stew

have been prepared. Ready to eat products viz: Murrel Wheat Kakhara, Murrel Ragi Kakhara,

Murrel Rice Kakhara, Murrel Pasta and Murrel Idli and Dosa have been prepared due to their

richness in nutritive properties for promoting growth of malnutrition babies and old people. A

combination of Murrel and Millet together as Murrel Millet Mix prepared is something unique to

improve health status of school children and aged people.

Responsible Partner: CIFA, Bhubaneswar, Partner Institute

In Orissa, snakeheads fetch relatively low price in comparison to other parts of the

country because of the religious taboo. Murrels are always sold live as they can survive in moist

condition for long time and their price is reduced by 30-40% when dead. In this context,

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imparting value addition to this fish will not only play an important role in employment

generation, but also income enhancement in rural sector. The entrepreneurs and SHGs, especially

women SHGs, can adopt this murrel processing technology to prepare several value added

products and supply them to the growing numbers of supermarkets and malls in the cities for

profitable earnings. Processed murrels are typically sold as dressed carcasses (head, viscera and

skin) removed or as boneless fillets. Murrels are excellent rounded meaty fish with single

skeleton and minimum intramuscular bones. Murrel meat is white, moist and resilient. Its firm

texture of flesh has a good meaty flavour. Murrels have an accumulation of fat deposits on the

lining of the abdominal cavity and in gastro-intestinal tract. Possession of thick belly muscle of

murrels helps in making good steaks of it. Processing and value addition of murrels into various

products viz. boneless fillets, chunks, nuggets, keema and steaks have been demonstrated to

farmers.

The carcass and commercial yield traits of C. striatus were evaluated. Experimental fish were

8-month-old snakehead murrels (C. striatus) weighing 500–700 gm, reared in earthen ponds and

cultivated intensively at a rate of 10,000 fingerlings/ha. The fish were given formulated feed for

a period of 1 year. Morphometric parameters were measured along with carcass, filleting, and

offal traits. Average meat-to-bone filleting ratio was 3.43 of marketable size murrels.

Insignificant accumulations of fat deposits on the lining of the abdominal cavity and coating the

bowels were noted.

Table 6 Carcass quality and yield traits

S. No. Murrel fish (Range 550-750g) Mean value (n=10)

1. Dressing percentage (%) 89.59

2. Dressing percentage of skinless round pieces (%) 80.80

3. Dressing percentage without Viscera and skin (%) 73.28

4. Dressing percentage without head, skin and viscera (%) 50.72

5. Average meat-to-bone filleting ratio 3.43

i. Value addition of Murrel

High perishability of fish and fish products is a serious problem in tropical countries like

India due to prevailing climatic conditions. Therefore, imparting value addition to this fish will

not only play an important role in developing self-stable products but also in employment

generation and income enhancement of the poor farmers.

Table 7 Sensory evaluation scores of murrel products (mean ± S.E)

S.

N

o

Produ

cts

Colour Flavour Texture Juiciness Sweetness Sourness Saltiness Overall

Acceptability

1 Curry 8.3±0.18 7.8±0.35 7.3±0.62 7.7±0.25 6.7±0.54 6.6±0.56 5.5±0.50 7.5±0.18

2 Finger

s

8.3±0.32 8.3±0.37 8.2±0.36 7.1±0.58 7.2±0.36 6.3±0.49 7.2±0.41 7.6±0.40

3 Fillets 8.0±0.32 8.1±0.29 8.1±0.39 7.2±0.52 6.6±0.45 6.0±0.49 6.2±0.52 7.7±0.25

4 Nugg

ets

7.8±0.29 7.7±0.36 7.8±0.35 6.6±0.46 7.0±0.50 6.1±0.44 7.1±0.29 7.6±0.26

5 Balls 6.7±0.67 7.8±0.35 7.6±0.32 0.7±0.25 7.1±0.35 7.0±0.32 6.5±0.26 7.8±0.22

6 Soups 6.7±0.67 7.0±0.65 7.0±0.50 6.7±0.70 5.8±0.58 5.7±0.41 5.1±0.51 7.3±0.18

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The entrepreneurs and SHGs, especially women SHGs and private farmers have been given

training on development of different murrel value added products such as murrel pickle, soup,

fingers, fillet, ball, steaks, curry, noodles and papadum.Sensory evaluation of murrel fish

products using a nine point hedonic scale indicated that murrel fish excelled in all the 6 types of

products.

ii. Quality and storage stability of low acid Murrel (C. striatus) pickle at room temperature

Emphasis is being given on developing self-stable fish and fish food products, which can

be stored at ambient temperature (32 + 0.50C). Fish pickling in vinegar and edible oil with added

salt, spices and condiments provide ready to eat highly acceptable convenience product with

good self-stability at an ambient temperature. Low moisture and reduced pH are the important

major factors contributing to the self-stability of the pickles (Frazier and Westhoff, 1984).

Pickling also helps in improving the desirable characteristics like taste and flavor along with the

preservative effect. The preliminary trials and evaluation of market fish pickle has indicated that,

with pH range 4.4 - 4.7, the products are objectionably sour; consequently its acceptability was

decreased. Information on development and storage stability of low acid murrel pickle is limited.

Therefore the objective of the present study was to prepare highly acceptable low acid murrel

pickle and study their storage stability at 32 +0.50C.

Low acid murrel meat pickle was prepared using deboned meat from marketable size

murrel (700 + 59 g). The product was evaluated after an elapse of seven days on maturation for

change in physico-chemical, microbiological and organoleptic properties at an interval of 15

days up to 60 days at 32 + 0.50C. Results showed that pH and titratable acidity of the low acid

pickle was 4.83 and 0.68, whereas in control murrel pickle, these values were 4.68 and 0.74

respectively. After 60 days of storage period microbiological count and sensory quality traits did

not show appreciable change and remained satisfactory throughout the storage period. Low acid

pickles had significantly lower sourness and high overall acceptability compared to the control.

Murrel pickle has the potential of becoming ready-to-eat, self-stable, highly acceptable health

food products of indigenous origin.

iii. Development of murrel gel and its application in making Papadum and Noodles

Washed pieces of murrel flesh were converted into gel by dilute acetic acid treatment. Murrel

flesh has excellent gel forming ability, emulsification and foaming properties. Murrel gel has

excellent functional properties. It has excellent keeping quality of 3 days at ambient temperature

(300C), 3 months under chilled condition and more than 6 months under frozen condition. Murrel

gel is highly nutritious and can be available on affordable price. Therefore, we have found its

application in development noodles and papadum.

6. Innovations

(Describe about the innovations and their impacts, one page each, please be clear about

innovation concept and describe only innovation(s) in brief)

I. Production Technology for Seed, Feed and Table size Murrels

a) Mass Seed Production of Murrels

Induced breeding of the striped murrel C. striatus and giant murrel C. marulius was

attempted using various natural and synthetic hormones. HCG 2000 IU / Kg has been

standardized for a single breeding set of striped murrel to produce 8,000 – 12,000 eggs.

f

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4,81,147 seeds of C. striatus and C. marulius were produced by lead and partner consortia

and stakeholders of Tamil Nadu and Orissa

b) Mass Culture of Live Feed

Succeeded in Mass culture of zooplankton, Vermiculture and Tufibex.

Culture of cladocerans and copepods (Ceriodaphnia cornuta, Moina micrura and Daphnia

carinata and Thermocyclops decipiens) was succeeded.

Density of zooplankton /litre: Cladocerans (8,000/l), Copepods – (7,000/l), Rotifers

(10,000/l) were produced in fiber tanks of 10,000 litres capacity.

c) Pelleted feed for murrels

There is no readymade feed for murrels in markets, since they are carnivorous, piscivorous

and cannibalistic. Formulated diet containing fish meal (anchovy and jawala) 60%, wheat

flour 10%, rice flour 10% and soya flour 20% respectively with 43% of protein was prepared

for murrels.

Feed pellets prepared using pelletizer ranged between 2.5 -5.0 mm were readily accepted by

murrel fingerlings. When they were trained.

To enhance the survival and growth and to improve the health of the fingerlings, the pellets

have been incorporated with herbs (Phyllanthus niruri, Aloe vera, Terminalia catappa,

Tinospora cardifolia, Withania sominifera etc) and probiotics (Bacillus subtilis,

Saccharomyces cervisiae, Lactobacillus helveticus, L. acidophilus, L. plantarum, L.

sporogens, Candida utilis etc).

Leaf powder of T. cardifolia (5 g/Kg feed) and root powder of W. sominifera incorporated in

fish feed (25g/Kg feed) resulted in cent percent survival in C. striatus fingerlings

d) Larviculture of Murrels:

Rearing of murrels from the post larvae to the fingerling stage needs extra care and is a

Herculean task.

Rotifers were recommended for 3 days old post larvae for 15 days.

Apart from zooplankton, the larvae were also fed on other live food organisms like blood

worms (Chironomus larvae), tubifex and earthworms.

Boiled chicken intestine paste and fish paste were used as feed for larvae from the 16th

day

onwards instead of live feed.

From 30th

day onwards, the fry were fed with cooked and minced chicken intestine and semi-

moist pellets and were later trained on pelleted feed (semimoist) upto fingerling stage.

e) Growout Culture of Murrels

Earthern ponds ranging from 0.1 ha to 0.4 ha with a depth of 1m are suitable for murrel

culture with a stocking density of 1/m2

Murrel farmers supply semimoist feed as dough in trays and gunny bags

52 farmers practiced murrel culture in Tamil Nadu and they harvested table size murrels in a

total area of 266.56 ha with a production of 27.67 tons in Tamil Nadu.

1119 farmers were trained for murrel culture through twenty one training and twelve

workshops in Tamil Nadu and Orissa

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The CIFA has developed it‟s grow out culture technology and it has been disseminated to the

farmers of Orissa under the NAIP project. The brief descriptions of the technology are as

follows:

Pond size: 0.1-0.2 ha.

Stocking size: (>10g) - To achieve better survival and production it is recommended to

stock ponds with advanced fingerlings.

Stocking density: 10,000-15,000 fingerlings/ha.

Feeding: boiled poultry viscera mixed with rice bran (8:2) – If poultry viscera are not

available then it can be replaced with trash fish accordingly (It is advisable to promote inherent

food production in the pond such as insect, weed fishes etc.)

Feeding rate: 2-5% of their body weight according to size.

Water quality parameters: As practiced for carps

Culture period: 8-10 months.

Harvesting: For total harvesting dewatering of pond is required and fishes are

handpicked.

II. Processing Technology for Fillet and Value added Products

a) Filleting Technology:

For the first time, the research team of CARE succeeded in preparation of fillet from live

as well as frozen murrel. Filleting technology yields 40% fillet, 23% head, 22% viscera, 8%

skin and 7% bone and the cost of fillet is Rs.800/Kg. Murrels are usually preferred as live fish

and this technology has a very good scope for preserving dead murrels in frozen form to prepare

fillet. Dead murrels were stored in ice boxes for 7 days whereas fillets were stored upto 30 days

in freezer (-4ºC). The beneficiaries were SHGs, Housewives and Star Hotels who can adopt this

processing technology to prepare several fast food items. Both CARE and CIFA demonstrated

fillet preparation, preparation of fast food items and canned items to the target groups.

Since the fillets are spine – free, children also show much interest in tasting the fish, thus

providing them with a protein rich diet. The prevailing compounds in murrel fillet were found

as 9-Octadecenoic acid (Z)-, methyl ester (56.69%), Hexadecanoic acid, methyl ester (21.02%),

Octadecanoic acid, methyl ester (10.74%), Arachidonic acid methyl ester (1.87%) and 9-

Hexadecenoic acid, methyl ester, (Z) (2.84%). The other compounds include Dodecanoic acid,

methyl ester (0.82%), Tetradecanoic acid (0.64%), 9,12,15-Octadecatrienoic acid, Methyl Ester,

(Z,Z,Z)(0.56%), Cyclopropaneoctanoic acid, 2-hexyl-, methyl ester(0.68%), Eicosanoic acid,

methyl ester (0.28%) and 8,11,14-Eicosatrienoic acid, (Z,Z,Z)(0.61%). The PUFA/ Saturated

(P/S) ratio of C. striatus fish oil is 0.69 which is higher than the Patin (Pangasius

hypothalamus) with 0.67. On the other hand, the ω-3/ ω-6 ratio of C. striatus is 0.96 which is

higher than the catfish, Clarias macrocephalus (0.25) and Patin (0.73).

b) Murrel Value Added Products:

More than 30 Value Added Products have been prepared and 6 CDs highlighting their

preparations have been developed. First Patent has been filed for “Murrel Cookies” which is

rich in protein and Polyunsaturated fatty acids (Per serving of Murrel cookies contain: Protein:

0.65 g; Calories: 53; Fat: 3.47g; Carbohydrates: 7.34g). The second patent on “Murrel Dhall

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33

Powder” a low fat food (Fat: 6.04%) and rich in calcium (800.8 mg/100g) and iron (229.5

mg/100g) was filed on 25.10.2013.

Treatment Technology for Wound, Arthritis, Convalescence, Ulcer and Inflammation

Murrels have medicinal value and are recommended for convalescence and arthritis

patients. In South-east Asian countries (Malaysia, Singapore, Vietnam, Thailand, etc.), the

murrel fish oil essence is commercially available and ingested by ladies after delivery for their

post-operative wound healing effects. Wound healing studies revealed faster healing upon topical

application of C. striatus crude extract by 21st day in 10% concentration whereas, 100% healing

was observed on 18th

day for standard treatment. Regarding anti-inflammatory studies, the

striped murrel extract at 400mg/kg dose showed significant decrease in the paw volume

(65.59%) of wistar rats when compared to the control Indomethacin. In anti-arthritis studies,

C. marulius rendered about 91.72% healing whereas C. striatus crude extract rendered 88.43%

healing in comparison with the Standard drug (92.86%). The anti-ulcer study revealed protection

against pyloric ligation and Ethanol induced ulcer in animal models. The results of our studies

revealed that murrels are bio-assets to the country and should be conserved for future generation.

7. Process/ Product/Technology/Value Chain/Rural Industry Developed

(List partner-wise major Process/ Product/Technology developed and their outcome in

quantifiable terms)

S.

No

(Process/Product/Technology/

Value Chain/ Rural Industry

Developed

Adoption/ Validation/ Commercialization,

etc.

Respons

ible

Partner

1 Murrel Seed Production Adopted, Validated and Commercialized

1,07,889 Seeds produced by SXC

2,63,258 Seeds produced by CIFA

1,10,000 Seeds produced by stakeholders

in Orissa

SXC &

CIFA

2 Grow out culture of murrel

(Technology)

Adopted, Validated and Commercialized

27.67 tons harvested in Tamil Nadu

2.5 tons harvested in CIFA

20.2 tons harvested in Orissa

SXC &

CIFA

3 Live Feed Culture Technology Adopted TNC

4 Larviculture (Technology) Adopted SXC

5 Pelleted Feed Technology Adopted SXC

6 Live Fish Transport (Technology) Attempted using Aluminium Drums, using

Anesthetics like clove oil

SXC

7 Murrel Filleting Technology Adopted, Validated and Commercialized SXC

8 Murrel Fish Gelatin (Products) Produced; Not yet adopted SXC

9 Value addition of murrels (ex.

Murrel Pickle and others)

(Products)

Adopted and Validated SXC &

CIFA

Note: Use pro-forma (1,2, 3) for details.

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34

8. Patents (Filed/Granted)

Responsible Partner: St.Xavier‟s College (SXC), Lead Institute

a). First Patent: Filed

Title of Patent: Nutritional Cookie Composition and A Process for Preparing the Same

Application No: Filed; 1277/DEL/2013dt. 30.04.2013

Name of Inventors:

1. M.A. Haniffa, Director, Centre for Aquaculture Research and Extension, St.Xavier‟s College,

Palayamkottai, Tirunelveli- 627002, Tamil Nadu

2. P. Jeya Sheela, Research Associate, ICAR-NAIP sub-project, Centre for Aquaculture

Research and Extension, St.Xavier‟s College, Palayamkottai, Tirunelveli- 627002, Tamil Nadu

3. H. Mohamed Riaz, Managing Partner, Kings Retail Ventures, No. 12, 18th

Cross street,

Maharaja Nagar, Tirunelveli- 627001, Tamil Nadu

b). Second Patent: Filed

Title of Patent: Process for Preparing Nutritionally Rich Dhal Powder and Its Composition

Application No: Filed; 3168/DEL/2013 dt.24/10/2013

Name of Inventors:

1. M.A. Haniffa, Director, Centre for Aquaculture Research and Extension, St.Xavier‟s College,

Palayamkottai, Tirunelveli- 627002, Tamil Nadu

2. P. Jeya Sheela, Research Associate, ICAR-NAIP sub-project, Centre for Aquaculture

Research and Extension, St.Xavier‟s College, Palayamkottai, Tirunelveli- 627002, Tamil Nadu

3. H. Mohamed Riaz, Managing Partner, Kings Retail Ventures, No. 12, 18th

Cross street,

Maharaja Nagar, Tirunelveli- 627001, Tamil Nadu

b). Third Patent: Under process, to be filed in March 2014

Title of Patent: Nutritionally Rich Murrel – Millet Mix

Name of Inventors:

1. M.A. Haniffa, Director, Centre for Aquaculture Research and Extension, St.Xavier‟s College,

Palayamkottai, Tirunelveli- 627002, Tamil Nadu

2. P. Jeya Sheela, Research Associate, ICAR-NAIP sub-project, Centre for Aquaculture

Research and Extension, St.Xavier‟s College, Palayamkottai, Tirunelveli- 627002, Tamil Nadu

3. Dr.H.B. Shivaleela, Professor, Food Science and Nutrition Department, University of

Agricultural Sciences, GKVK Campus, Bangalore

9. Linkages and Collaborations *

S. No. Linkages developed

(Name & Address of Organization)

Number of

Linkages

Responsible

Partner

1 Linkages within India 25 SXC

2 Linkages Outside India 15 SXC

3 Linkages with Fish Vendors 12 SXC

4 Women SHG‟s

SXC - 1

CIFA - 8

SXC &

CIFA

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35

5 Male SHGs 1 CIFA

6 NGO 1 CIFA

7 Govt. Organizations 5 CIFA

8 Private Farmer

SXC - 53

CIFA - 7

SXC &

CIFA

*(Please refer Annexure III for details)

10. Status on Environmental and Social Safeguard Aspects

(Please see NAIP website for clarity on the subject)

11. Constraints, if any and Remedial Measures Taken: Nil

Difficulty in getting qualified and retention of the scholars throughout the project period of 3-

5years.

Environmental Safeguards

S. No. Environmental Issue (if any) Mitigation measures

1. Draining of stagnant water Used for irrigation purposes

2. Fertilization of ponds Used in an eco friendly way as organic manure

2. Disposal of agrowaste Used for fish feed preparation

3. Mass number of dead fish Preparation of murrel fillet

Fillets used for preparation of ready to eat and ready to

cook products.

Utilization of head and central spine for soup preparation

Skin used for leather manufacture and gelatin extraction

Viscera used for manures after decomposition

4. Mass number of diseased fish Used as manures after decomposition

Social safeguard

1. Lack of knowledge of murrel

culture

Murrel culture popularized and about 1119 farmers were

trained

2. Farmers regret for

diversification of murrel

culture from carp culture

Farmers motivated through 21 training and 12 workshop

3. Lack of awareness on Income

generation

Market assurance, value added products, and consumer

preference

4. Lack of knowledge on

medicinal and recuperative

values of Murrel dishes

Promoted consumer awareness – Murrel Fish Expo

conducted; Articles published

5. Lack of knowledge on Murrel

dishes and murrel products

Value added products popularized – Nellai Fish Food

Festival and Nanjil Fish Food Festival conducted during

February and March 2013

6. Monitory loss to fish vendors

by fish mortality

Stakeholders trained for processing and value added products

- Fish vendors yet to be trained.

7. Lack of knowledge on

nutritional status of murrels

Nellai Fish Food Festival and Nanjil Fish Food Festival

conducted during February and March 2013

8. Religious taboo for

C. marulius in Orissa

Farmers advised to supply to adjacent states as live and

processed by CIFA

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36

Continuous monsoon failure for the past three years resulted in insufficient water supply for

murrel farming in Tamil Nadu.

Psychological fear of farmers towards murrel culture and their change in attitude towards one

practice to another practice

12. Publications (As per format of citation in Indian Journal of Agricultural Sciences)

A. Research papers in peer reviewed journals. Details as per the guidelines for citation of

publications (Annexure I)

S.

No.

Authors, Title of the paper,

Name of Journal, Year, Vol. & Page No.

NAAS

Ratings

(NR)

Responsi

ble

Partner

1 Dhanaraj M, Haniffa M A, Arunsingh V, Muthu Ramakrishnan

C, Manikandaraja D and James Milton M. 2009. Antibacterial

activity of skin and intestinal mucus of five different murrel

species. Malaysian Journal of Science 28(3): 257-262.

NR: Nil

SXC

2 Mehraj Ud Din W, Altaff K and Haniffa M A. 2009. Study on the

growth and survival of Channa striatus post larvae (Bloch) using

live feed. Bangladesh Journal of Fisheries Science 13(2): 131-

135.

NR: Nil

TNC

3 Mehraj Ud Din W, Altaff K and Haniffa M A. 2009. Comparative

study on growth of Channa striatus fry and fingerlings using

different feeding regimes. Journal of Aquatic Biology 24(2):

173-176.

NR: Nil

TNC

4 Altaff K and Mehraj Ud Din W. 2010. Culture of Ceriodaphnia

cornuta using chicken manure as fertilizer: Conversion of a

Waste product into highly nutritive protein. Pakistan Journal of

Scientific and Industrial Research 53(2): 89-91.

NR: Nil

TNC

5 Mehraj Ud Din W and Altaff K. 2010. Culture of zooplankton for

rearing fish larvae. Pollution Research 29(2): 91-93.

NR: 4.75

TNC

6 Mehraj Ud Din W, Altaff K and Haniffa M A. 2010. Evaluation

of growth and survival of fry of Channa striatus (Bloch) using

different feeding regimes and cladoceran live feed. Journal of

Experimental Zoology India 13(1): 225-227.

NR: 4.63

TNC

7 Mehraj Ud Din War and Kareem Altaff. 2011. High density

culture of a cladoceran Moina micrura (Kurz, 1874) by utilizing

poultry manure. Pakistan Journal of Scientific and Industrial

Research 54(3): 131-135.

NR: Nil

TNC

8 Mehraj Ud Din War, Kareem Altaff and Mohammed Abdul

Khader Haniffa. 2011. Growth and survival of larval snakehead

Channa striatus (Bloch, 1793) fed different live feed organisms.

Turkish Journal of Fisheries and Aquatic Sciences 11(3):523-

528.

NR: 6.59

IF*: 0.591

TNC

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37

9 Muthukrishnan Dhanaraj and Mohamed Abdul Kather Haniffa

2011. Effect of probiotics on growth and microbiological

changes in snakehead Channa striatus challenged by Aeromonas

hydrophila. African Journal of Microbiology Research

26(5):4601-4606.

NR: 6.0

IF : 0.54

SXC

10 Rauta P R, Kumar K and Sahoo P K. 2011. Emerging new multi-

drug resistant bacterial pathogen Acinetobacter baumanii from

snake head Channa striatus from India. Current Science 101(4):

548-553

NR: 6.91

IF: 0.905

CIFA

11 Sahu B B, Kumar K, Sahu A K, Kumar R, Mohanty U L, Jahan

N, Sahoo M and Eknath A E. 2012. Carcass Characteristics of

Marketable size Striped Murrel Channa striatus (Bloch, 1793).

Journal of Applied Ichthyology 28(2): 258-260.

NR: 6.90

IF: 0.902

CIFA,

12 Sahu B B, Kumar K, Sahoo A K, Kumar R, Mohanty U L, Majhi

U J, Jahan, N, Sahoo M and Jayasankar P. 2012. Quality and

storage stability of low acid murrel (Channa striatus) pickle at

room temperature. International Food Research Journal 19(4):

1629-1632.

NR: Nil

CIFA

13 Haniffa M A and Kavitha K. 2012. Antibacterial activity of

medicinal herbs against the fish pathogen Aeromonas

hydrophila. Journal of Agricultural Technology 8(1): 205-211.

NR: Nil

SXC

14 Haniffa M Aand Shanthi P. 2012. Phytochemical analysis and

antibacterial screening of medicinal plants against Aeromonas

hydrophila. Asian Journal of Pharmaceutical and Clinical

Research

NR: Nil

SXC

15 Bilal Ahmad Paray, Haniffa M A and Manikandaraja D. 2012.

Induced ovulation and spawning of a striped snakehead murrel

Channa striatus (Bloch) under captive conditions. Journal of

Research in Animal Sciences 1(1): 033-039.

NR: Nil

SXC

16 Kuldeep K, Lalrinsanga P L, Sahoo M, Mohanty U L, Kumar R

and Sahu A K. 2013. Length-Weight Relationship and Condition

Factor of Anabas testudineus and Channa Species under

Different Culture Systems. World Journal of Fisheries and

Marine Science 5(1): 74-78.

NR: Nil

CIFA

17 Haniffa M A, Jeya Sheela P, James Milton M and John De Britto

A. 2013. Invitro and Invivo Antimicrobial Effects of Wrightia

tinctoria (Roxb.) R. Br. against Epizootic Ulcerative Syndrome

in Channa striatus. International Journal of Pharmacy and

Pharmaceutical sciences 5(3): 219-222.

NR: Nil

SXC

18 Haniffa M A, Kavitha Bharathi B, Viji Margaret I, Bilal Ahmad

Paray and Manikandaraja D. 2013. Effect of probiotics and

herbal additives on growth, survival and disease resistance of

NR: Nil

SXC

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38

striped murrel. World Aquaculture 44(2): 64-67.

19 Haniffa M A, Jeya Sheela P and James Milton M. In vitro

antibacterial activity of tissue extracts from four channids

against enteric pathogens. Journal of Agricultural Technology

9(6): 1437-1445.

NR: Nil

SXC

20 Ramesh S, Chelladurai G and Haniffa M A. 2013. Isolation of

enzyme producing bacterial from gut of Channa striatus fed on

different herbs and probiotics diet. International Journal

Pharmacy and Pharmaceutical Sciences 5(4): 195-198.

NR: Nil

SXC

21 Mehrajuddin War and Kareem Altaff. 2013. Preliminary Studies

on the Effect of Prey Length on Growth, Survival and

Cannibalism of Larval Snakehead, Channa striatus (Bloch,

1793). Pakistan Journal of Zoology 45(2): 1-6.

NR: Nil

TNC

22 Bilal Ahmad Paray, Haniffa M A, Manikandaraja D and James

Milton M. 2013. Breeding Behaviour and Parental Care of the

Induced Bred Striped Murrel Channa striatusunder Captive

Conditions. Turkish Journal of Fisheries and Aquatic Sciences

13: 707-711.

NR: 6.59

IF*: 0.591

SXC

23 Mohapatra S D, Kumar K, Jayasankar P and Barman H K. 2013.

Establishment of dry – down hypoxic stress treatment protocol

for snakehead freshwater fish, Channa striatus. International

Journal of Fisheries and Aquatic Sciences 1(2): 36–39.

NR: Nil

CIFA

Papers in Press

1 Haniffa M A, Jeya Sheela P, James Milton M and Kavitha K

2012. Protein Profiling for Phylogenetic Relationship of

Snakehead Species. Iranian Journal of Fisheries and Aquatic

Sciences

NR: Nil

IF*: 0.614

SXC

2 Haniffa M A, Jeyasheela P, Kavitha K, Mat Jais A M. 2013.

Salutary values of striped snakehead- Channa striatus – A

review. Asian Pacific Journal of Tropical Biomedicine

NR: Nil

SXC

3 Haniffa M A, Viji Margaret I, Kavitha Bharathi B and Jeya

Sheela P. 2013. Dietary administration of herbal additive

Phyllanthus niruri on growth performance and haematological

changes in striped snakehead Channa striatus. World

Aquaculture

NR: Nil

SXC

4 Mehrajuddin War and Kareem Altaff. Utilization of Poultry

excreta for high density production of Daphnia carinata (King

1853). Cost effective and environmental friendly technique.

Pakistan Journal of Scientific and Industrial Research

NR: Nil

TNC

5 Haniffa M A, Shanthi P, Kavitha K, Manikandaraja D and Jeya

Sheela P. 2013. Value Addition of Murrels: a Boon to Fish

Farmers. Fishing Chimes

NR: Nil

SXC

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39

Papers communicated

1 Haniffa M A, Jeya Sheela P, Vigneswaran R and Kavitha K.

2012. Effect of Vitamins on Digestive Enzyme Activities and

Growth Performances of Striped Murrel Channa striatus.

Iranian Journal of Fisheries and Aquatic Sciences

NR: Nil

SXC

2 Haniffa M A, Jeya Sheela P, James Milton M, Kavitha K and

Ajaz Ali Bhat. 2012. Inter simple sequence repeat (ISSR)

marker and principal component analysis (PCA) for species

identification and evolutionary analysis. Biochemical

Systematics and Ecology

NR: 7.15

SXC

3 Haniffa M A, Jeya Sheela P and Kavitha K. 2012. Anesthetic

Efficacy of Clove Oil and its Impact on Hematological and

Biochemical Changes in Channa striatus (Bloch, 1793).

Turkish journal of Fisheries and Aquatic Sciences

NR: 6.59

IF*: 0.591

SXC

4 Bilal Ahamad Paray, Haniffa M A and Ananth Kumar Y.

Studies on Embryonic and Larval Development of Induced

Bred Channa striatus. Envirobiotech Journal

NR: Nil

SXC

5 Ajaz Ali Bhat, Haniffa M A, James Milton M, Bilal Ahmad,

Divya P R, Gopalakrishnan A. 2013. Genetic variation of

Striped Snakehead (Channa striatus, Bloch, 1793) populations

using RAPD markers. International Journal of Biodiversity

and Conservation

NR: Nil

SXC

*IF: Impact Factor

B. Books/ Book chapters/ Abstracts/ Popular articles, Brochures, etc.

S.

No.

Authors, Title of the papers

Name of Book/ Seminar/ Proceedings/Journal, Publisher, Year, Page No.

Respon

sible

Partne

r

1. Books

1 Sundararaj V and Haniffa M A. 2010. Veral Valarppu, p 248 Naveena

Velaanmai, Chennai, India

SXC

2 Haniffa M A. 2011. Aquatic Resources and Aquaculture, p 328 Dominant

Publishers and Distributors Pvt. Ltd., New Delhi, India

SXC

3 Sundararaj V. and Altaff K. 2012. Meen Valarpil Uyirunavugal, p 258 Naveena

Velanmei, Chennai, India

TNC

4 K. Kumar, R. Kumar, S. Saurabh, M. Sahoo, A. K. Mohanty, P. Lalrinsanga, U.

L. Mohanty, A. K. Sahu and P. Jayasankar. 2012. Snakehead: Fishes fact sheets.

p 38 Central Institute of Freshwater Aquaculture (ICAR), Kausalyaganga,

Bhubaneswar

CIFA

2. Book Chapters

1 Eknath A E, Kumar K, Jena J K, Mohanty U L, Kumar R, Jahan N and Sahoo M.

2010. Murrel: An ideal fish for mass production (in) souvenir of Assam Matsya

CIFA

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40

Mahotsav, organized by the Department of Fisheries, Govt. of Assam during 30th

Jan- 1st Feb 2010. p 5-8

2 Kumar K, Sahu A K, Kumar R, Mohanty U L, Sahoo M and Jahan, N. 2011.

Murrel Culture in Odisha-A Humble Beginning (in) Aquaculture Innovators, p

105-112, Radheshyam et al. (Eds), Central Institute of Freshwater Aquaculture,

Kausalyaganga, Bhubaneswar, India.

CIFA

3 Kumar K, Kumar R, Mohanty U L and Sahu A K. 2012. Murrel culture and its

popularization. (in) Training manual on Fish Breeding and Seed Production,

p37-45, KVK, CIFA (Eds), Department of Fisheries, Govt. of Odisha and KVK-

Khurda, Central Institute of Freshwater Aquaculture, Kausalyaganga,

Bhubaneswar, India.

CIFA

4 Kumar R, Kumar K, Mohanty U L and Sahu A K. 2012. Murrel (Channa

striatus): Potential candidate species for nutritional security and livelihood

generation. (in) Souvenir: The world food day, p 49-51, S J Swain et al. (Eds),

Odisha Krishak Samaj, Bhubaneswar, Odisha

CIFA

5 Kumar K, Kumar R, Mohanty U L, Saurabh S, Sahu B B, Sahoo M, Mohanty A,

Sahu A K and Jayasankar P. 2013. Snakehead fishes: Alternative candidate fish

for diversification aquaculture. pp- 119-132 (in) Aquaculture: New Possibilities

and Concerns, P-187 Sinha, V.R.P., Jayasankar, P. (Eds), Narendra Publishing

House, Delhi, India.

CIFA

6 Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) Ka beej utpadan

ebm Palan (Hindi) (Seed Productuction and culture of murrel) (in) Training

manual on Jalkrishi mein Dakhata Vikash, p 50-54. Published by Director,

CIFA, Kausalyaganga, Bhubaneswar, India on 15-19 July 2013.

CIFA

7 Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) Ka beej utpadan

ebm Palan (Hindi) (Seed Productuction and culture of murrel) (in) Training

manual on Unnat mithajal matya palan, p 54-57. Published by Director, CIFA,

Kausalyaganga, Bhubaneswar, India. 12-16 December 2013

CIFA

8 Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) ka beej utpadan

ebm Palan (Hindi) (Seed Productuction and culture of murrel) (in) Training

manual on ‘Mithajal Matya palan mein unnat praudhogiki’, p56-59. Published

by Director, CIFA, Kausalyaganga, Bhubaneswar, India. 20-24 December 2013

CIFA

3. Manual

1 Haniffa M A, Dhanaraj M, Muthu Ramakrishnan C, Arunsingh V and

Manikandaraja D. 2009. Veral Meen Valarpu (Tamil) Murrel Fish Culture

Centre for Aquaculture Research and Extension (CARE), St.Xavier‟s College,

Palayamkottai, Tamil Nadu, India, p 20

S

X

C

2 Kumar K, Kumar R, Mohanty U L, Jahan N, Sahoo M and Sahu A K 2010.

(Odia) (Murrel Seed Production and Culture) Central Institute of Freshwater

Aquaculture, Bhubaneswar, India, p20

CIFA

3 Kumar K, Sahu B B, Kumar R, Mohanty U L, Jahan N, Sahoo M and Sahu A K

2010. Value Addition: Flavours from Murrel (bilingual) Central Institute of

CIFA

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41

Freshwater Aquaculture, Bhubaneswar. p32

4 Kumar K, Kumar R, Mohanty U L, Sahoo M and Sahu A K. 2011. Murrel

farming- A manual for farmers. Central Institute of Freshwater Aquaculture,

Kausalyaganga, Bhubaneswar, India, p13

CIFA

5 Kumar K, Kumar R, Mohanty U L, Sahoo M, Mohanty A K and Sahu A K. 2012.

Murrel Culture and its popularization. Central Institute of Freshwater

Aquaculture, Kausalyaganga, Bhubaneswar, India, p25.

CIFA

6 Kumar R, Mohanty U L, Sahoo M, Sahu A D and Sahu A K. 2013. Murrel

Machha chasa, prajanana o janla utpadana (Odia) (Breeding, Seed production

and culture of murrel). Central Institute of Freshwater Aquaculture,

Kausalyaganga, Bhubaneswar, India, p25

CIFA

4. Abstracts

4.1 Papers Presented in International Conferences Abroad

1 Haniffa M A. 2010. Effect of Effinol ® FG incorporated diets on growth response

of Striped Snakehead Channa striatus (in) Proceedings of International

Conference on World Aquaculture 2010 - General Aquaculture session held

during 1 – 5 March 2010 at San Diego, California.

SXC

2 Haniffa M A. 2011. Effect of Probiotics on growth and microbiological changes

in snakehead Channa striatus challenged by Aeromonas hydrophila (in)

Proceedings of International Conference on Aquaculture America 2011 - Fin

Fish Nutrition session held during 28th

February – 3rd

March 2011 at New

Orleans Marriott, New Orleans, Louisiana.

SXC

3 Haniffa M A and Abdul Kader Mydeen K P. 2012 Effects of medicinal herbs on

haematological and biochemical changes in Channa punctatus, Heteropneustes

fossilis and Cyprinus carpio challenged by Aeromonas hydrophila (in)

Proceedings of International Conference on AQUA 2012, held during 1 – 5

September 2012 at Prague Czech Republic

SXC

4 Haniffa M A and Abdul Kader Mydeen.K P. Isolation and identification of

microflora Of Diseased Channa punctatus, Heteropneustes fossilis and Cyprinus

carpio and Treatment By Medicinal Herbs (in) Proceedings of International

Conference AQUA 2012, held during 1 – 5 September 2012 at Prague Czech

Republic

SXC

4.2 Abstracts in National Conferences

1 Kumar K, Damle, D K, Jahan N, Sahoo M, Mohanty U L, Kumar R. 2010.

Formulation of cost effective feeds from locally available animal based

ingredients for Channa striatus fingerlings (in) Golden Jubilee National Seminar

on Diversification of Aquaculture through locally available fish species (DALAF-

2010) held on 27-28 August 2010 at CIFE Centre, Kolkata, India

CIFA

2 Kumar K, Mohanty U L, Kumar R, Jahan N, Damle, D K and Sahoo M. 2011.

Production of snakeheads with different stocking densities in pond ecosystem.

(in) Proceedings of International Conference on Aquaculture Asia held during

17-21 January 2011 at Kochi, India.

CIFA

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42

3 Manikandaraja D. 2011. Morphometric and Meristic Development of Larval and

Juvenile Snakehead Channa striatus (Bloch 1793) (in) Proceedings of

International Conference on Asian Pacific Aquaculture, held during 17-20

January 2011Kochi, Kerala, India

SXC

4 Bilal Ahmed Paray. 2011. Induced breeding and seed production of striped

murrel Channa striatus (in) Proceedings of International Conference on

Aquaculture Asia held during 17-21 January 2011 at Kochi, India.

SXC

5 Mohanty U L, Kumar R, Jahan N and Sahoo M. 2011. Effect of medicinal plant

crude extracts on the health of Channa striatus fingerlings (in) Proceedings of

National Conference on 9th

Indian Fisheries Forum held during 19-23 December

2011 by CMFRI at Chennai, India.

CIFA

6 Kumar K, Sahoo M, Jahan N, Sahu A K, Kumar R, Mohanty U L and Eknath A

E. 2011. Murrel Culture-A panacea for the Farmers (in) Proceedings of National

Conference on 13th

Indian Agricultural Scientists and Farmers’ Congress held

during 19-20 February 2011 at Allahabad, India

CIFA

7 Hameedullah Sherief S, Haniffa M A, Kavitha K and Riyaz Ahmed A. 2012.

Molecular identification of Epizootic Ulcerative Syndrome (EUS) in striped

murrel Channa striatus (in) Proceedings on National Conference on

Biotechnological Approaches in Aquaculture, Live Aqua 2012, held during 1-3

February 2012, School of life Sciences, Bharathiyar University, Coimbatore,

Tamil Nadu, India

SXC

8 Kavitha K, Haniffa M A and Sunitha K S. 2012. Prophylaxis and treatment of

Epizootic Ulcrerative Syndrome (EUS) in murrels (in) Proceedings on National

Conference on Biotechnological Approaches in Aquaculture, Live Aqua 2012,

held during 1-3 February 2012, School of life Sciences, Bharathiyar university,

Coimbatore, Tamil Nadu, India

SXC

9 Manikandaraja D, Haniffa MA and Shanthi P. 2012. Length – Weight

Relationship and Condition Factor of Captured and Cultured Snakehead Channa

striatus(in) Proceedings on National Conference on Biotechnological

Approaches in Aquaculture, Live Aqua 2012, held during 1-3 February 2012,

School of life Sciences, Bharathiyar university, Coimbatore, Tamil Nadu, India

SXC

10 Kumar R, Mohanty U L, Kumar K, Sahoo M, Sahu A K, Jena J K and

Jayasankar, P. 2012. Murrel Farming: A New initiative in Odisha (in)

Proceedings of International Conference on Global Symposium on Aquatic

resources for eradicating hunger and malnutrition-opportunities and challenges

held during 4-6 December 2012 at Mangalore Fisheries College, Mangalore,

India

CIFA

11 Kumar R. 2012. Possibilities of murrel culture in Northern India (in) Proceedings

of International Conference on 14th

Indian Agricultural Scientist and Farmers’

Congress on Agriculture and Agripreneurship organized by Bioved research

CIFA

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43

society, held during 18-19 February 2012 at Allahabad, U.P

12 Haniffa M.A. 2012. Problems and Challenges of Indian Aquaculture and

Attempts by CARE- A Review (in) Proceedings of National Conference on

Indian Science Congress Association, Regional Science Congress meet,

Coimbatore Chapter, held during 15 & 16th

December 2012, Kongunadu Arts

and Science College, Coimbatore, Tamil Nadu, India.

SXC

13 Bilal Ahmad Paray and Haniffa M.A. 2012. Effect of certain feeds on growth

and survival of striped snakehead, Channa striatus (Bloch, 1793) hatchlings as

test starter diets under captive conditions (in) Proceedings of National

Conference on Indian Science Congress Association, Regional Science Congress

meet, Coimbatore Chapter, held during 15 & 16th

December 2012 Kongunadu

Arts and Science College, Coimbatore, Tamil Nadu, India.

SXC

14 JeyaSheela P, Haniffa M.A and Kavitha K. 2012. Medicative Values of Striped

Murrel, Channa striatus(in) Proceedings of National Conference on Indian

Science Congress Association, Regional Science Congress meet, Coimbatore

Chapter, held during 15 & 16th

December 2012, Kongunadu Arts and Science

College, Coimbatore, Tamil Nadu, India

SXC

15 Haniffa M A. 2013. Biodiversity and Food security (in) Proceedings of National

Conference on Food security Issues and Concerns, held during 21- 22 February,

2013, Arulanandar College, Karumathur, Tamil Nadu, India

SXC

5. Popular articles

1 Haniffa M A. 2009. ICAR NAIP sponsored workshop on breeding and seed

production of murrels. Fishing Chimes 29(8): 48 -51.

SXC

2 Haniffa M A, Dhanaraj M, Muthu Ramakrishnan C, James Milton M, Arunsingh

S V and Ajaz Ali. 2009. Backyard Murrel Culture for Women. Infofish

International 6:14 -16.

3 Haniffa M A, Dhanaraj M, Muthu Ramakrishnan C, Arunsingh V and

Manikandaraja D. 2010. Fecundity of C. striatus (Bloch 1793) collected from

Tambraparni River. Fishing Chimes 30(7): 7-8

SXC

4 Haniffa M A, Bilal Ahmad Paray and Manikandaraja D. 2010. Trainings on Seed

production, feed formulation, and culture of murrels. Fishing Chimes 30(8): 35-

38.

SXC

5 Haniffa M A, Bilal Ahmad Paray and Manikandaraja D. 2010. ICAR-NAIP sub-

project workshops on seed production, feed formulation and disease management

in murrel culture. Fishing Chimes 30(8): 39-42

SXC

6 Haniffa M A. 2010. Viru Viru Labam Tharum Viral Valarppu (Tamil) (Murrel

Fish Culture for Fast Income). Pasumai vikadan, 10.11.2010, p 4-9

SXC

7 Haniffa M A, Seenappa D, Manikandaraja D and James Milton M. 2011. Murrel

Farming training to Fish farmers of Karnataka. Fishing Chimes 30(10&11): 52-

53.

SXC

8 Kumar K, Eknath A E, Sahu A K, Mohanty U L, Kumar R, Sahoo M and Jahan N.

2011. Snake heads: Challenging fish for diversification of fish farming. Fishing

CIFA

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44

Chimes 31(1): 110-113.

9 Sahu B B, Kumar K, Sahu A K, Mohanty U L, Kumar R, Sahoo M, Jahan N and

Eknath A E, 2011. Processing and value addition to murrel in value chain.

Fishing Chimes 31(1): 106-108.

CIFA

10 Haniffa M A. 2011. Popularization of Murrel Farming among Fish Farmers of

Hyderabad. Fishing Chimes 31(2): 27-29

SXC

11 Haniffa M A. 2011. A Value Chain on Murrel Production for Income Generation.

Fishing Chimes 31(5): 19-21

SXC

12 Haniffa M A. 2011. Villangam Illatha Viral Valarppu (Tamil) (Harmless Murrel

Fish Culture). Pasumai vikadan, 25.09.2011, p 40-41

SXC

13 Haniffa M A. 2011. Viral Meen Valarppu Payirchi Mugaam (Tamil) (Murrel Fish

Training). Valarum Thamilagam, November 2011, p 34-35.

SXC

14 Haniffa M A. 2011. Pambuthalai Viralmeen, Meen Valarporukku Oru

Varaprasatham (Tamil) (Snakehead murrel fish culture- a boon to fish farmers).

Valarum Thamilagam, December 2011, p 36-37

SXC

15 Haniffa M A. 2011. Veeru Nadai Podum Viral Valarpu (Tamil) (Fast Growing

Murrel Culture). Pasumai vikadan, 10.12.2011, p 28-31.

SXC

16 Kumar K, Kumar R, Mohanty U L and Sahu A K. 2012. Murrel Maccha Chasa O

tara Prasara (Odia) (Murrel farming and its popularization). Meenalok,

FISHCOPFED, Govt. of India, October-December 2012, p3-6.

CIFA

17 Haniffa M A. 2012. Thennaiku Idail Viral (Tamil) (Murrel Culture amidst

Coconut Trees). Pasumai vikadan, 10.02.2012, p 29-31

SXC

18 Haniffa M A. 2012. ICAR- NAIP Sponsored One day Training Programme on –

Seed Production, Feed Formulation and Culture of Murrels: 22 Sept 2011.

Fishing Chimes 31(12): 18-20

SXC

19 Haniffa M A and Mohamed Riyaz H. 2012. Murrel Fish Food Expo 2012. Fishing

Chimes 32(5): 9-12

SXC

20 Haniffa M A. 2013. Mana Aluthama, Viral Meen Biscuit Sapidunga (Tamil)

(Murrel biscuits a cure for depression). Kungumam, 26.08.2013, p 95-96.

SXC

21 Haniffa M A, Jeyasheela P, Manikandaraja D, Jafar Sathik S, and Bilal Ahmad.

2013. Nellai Fish Food Festival 2013. Fishing Chimes 33(6): 18-24

SXC

22 Haniffa M A, Jeyasheela P, Manikandaraja D, Jafar Sathik S, and Bilal Ahmad.

2013. Nanjil Food Fish Festival 2013. Fishing Chimes 33(6): 25-28

SXC

23 Kumar K, Kumar R, Mohanty U L and Sahu A K. 2013. Sal seula macchara janla

utpadan (Odia) (Seed production of murrel) Meenalok FISHCOPFED, Govt. of

India, January-March 2013, p 10-13.

CIFA

6. Brochures

1 Kumar K, Sahu A K, Kumar R, Mohanty U L, Sahoo M, Jahan N, Jena, J K and

Eknath A E. 2010. Farming of Snakeheads. CIFA, Bhuabneswar

CIFA

2 Kumar K, Sahu B B, Kumar R, Mohanty U L, Sahoo M, Jahan N, Sahu A K,

Jena, J K and Eknath A E. 2010. Processing and value addition of murrel. CIFA,

CIFA

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45

Bhuabneswar

7. CDs/ Videos produced by Lead Consortia, Centre for Aquaculture Research and

Extension, St.Xavier‟s College (SXC), Tamil Nadu

1 Haniffa M A. 2010. ICAR- NAIP Sub project activities – 2 minute Video Coverage (2009 -

2010).

2 Haniffa M A. 2010. Murrel Culture Training Programme - Hyderabad, Chennai and

Coimbatore.

3 Haniffa M A. 2010. Murrel Seed Production at CARE Aquafarm.

4 Haniffa M A. 2010. Murrel Seed Production at Vallanadu Fish Farm.

5 Haniffa M A. 2010. Pelleted Feed Preparation for Large and Small Scale Murrel Culture

6 Haniffa M A. 2010. Murrel Feeding at Vallanadu Fish Farm.

7 Haniffa M A. 2010. Murrel Growout Culture at High Ground Aquafarm – Tirunelveli.

8 Haniffa M A. 2011. ICAR- NAIP Sub project activities – 2 minute Video coverage (2010 -

2011).

9 Haniffa M A. 2011. Murrel Seed Production at Araikulam Fish Farm.

10 Haniffa M A. 2011. Murrel Parental Care at Araikulam Fish Farm.

11 Haniffa M A. 2011. Semi Moist Feed Preparation for Murrel Culture.

12 Haniffa M A. 2011. Murrel Table – Size Fish Production at CARE.

13 Haniffa M A. 2011. Murrel Growout Culture at Arasan Aquafarm – Araikulam.

14 Haniffa M A. 2011. Murrel Harvest at Araikulam Fish Farm.

15 Haniffa M A. 2011. Murrel Harvest at Kaliyavoor Fish Farm.

16 Haniffa M A. 2011. Murrel Fillet Preparation.

17 Haniffa M A. 2011. Murrel Value Addition (Murrel Briyani, Cutlet, Fingerchips, Pickle).

18 Haniffa M A. 2012. Murrel Harvest at Araikulam Fish Farm.

19 Haniffa M A. 2012. Murrel Harvest at Veeralaperunselvi Fish Farm.

20 Haniffa M A. 2012. Murrel Fish Food Expo 2012.

21 Haniffa M A. 2013. Murrel Fish Cookies.

22 Haniffa M A. 2013. Mass Culture of Live Feed.

23 Haniffa M A. 2013. Nellai Fish Food festival 2013

24 Haniffa M A. 2013. Nanjil Fish Food Festival 2013

7.2 CDs/ Videos produced by Partner Institute, CIFA, Orissa

1 Sahu B B, Kumar K, Kumar R, Mohanty U L, Sahu A K, Sahoo M, Jahan N and Eknath A E,

2010. Value addition of murrel.

13. Media Products Developed/Disseminated

S.

No.

CD, Bulletins, Brochures, etc.

(Year wise)

No. of

Copies

Distrib

ution

Respons

ible

Partner

1 Pamphlet on “Farming of Snakeheads” was released by the

honourable Chief Minister, Assam on the occasion of Assam

250 240 CIFA

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46

Matsya Mahotsav, organized by the Department of Fisheries,

Govt. of Assam during 30th

Jan- 1st Feb 2010

2 A training manual (in Oriya) on “Murrel Seed Production

and Culture” has been developed and it was released by the

Director, Central Institute of Freshwater Aquaculture,

Bhubaneswar 2010

150 130 CIFA

3 A pamphlet on “Processing and value addition of murrel” in

2010

300 275 CIFA

4 A training manual (bilingual) on “Value Addition: Flavours

from Murrel ” has been developed and it was released by the

Director, Central Institute of Freshwater Aquaculture,

Bhubaneswar.2010

25 20 CIFA

5 Fact sheet on murrels has been released by Honorable

Fisheries Minister Govt. of Assam on 6th

December 2010.

60 50 CIFA

6 CD on “Value addition of murrel” has been developed on

2010

25 20 CIFA

7 „MURREL FARMING- A manual for farmers‟ was

developed and it has been released by Secretary, Department

of Fisheries, Govt. of Assam, Guwahati during a Workshop

conducted in Assam on 11th

September 2011.

60 50 CIFA

8 Training manual on “Murrel culture and its popularization”

in Oriya, 2012

70 60 CIFA

9 Training manual on „Murrel Machha chasa, prajanana o janla

utpadana (odia) (Breeding, Seed production and culture of

murrel).2012

35 25 CIFA

10 CD on Murrel Seed Production at CARE Aquafarm. 600 595 SXC

11 CD on Murrel Seed Production at Vallanadu Fish Farm. 600 595 SXC

12 CD on Pelleted Feed Preparation for Large and Small Scale

Murrel Culture.

600 595 SXC

13 CD on Murrel Feeding at Vallanadu Fish Farm. 600 595 SXC

14 CD on Murrel Growout Culture at High Ground Aquafarm –

Tirunelveli

600 595 SXC

15 CD on Murrel Seed Production at Araikulam Fish Farm. 600 595 SXC

16 CD on Murrel Parental Care at Araikulam Fish Farm. 600 595 SXC

17 CD on Semi Moist Feed Preparation for Murrel Culture. 600 600 SXC

18 CD on Murrel Table – Size Fish Production at CARE. 600 550 SXC

19 CD on Murrel Growout Culture at Arasan Aquafarm –

Araikulam.

600 550 SXC

20 CD on Murrel Harvest at Araikulam Fish Farm. 600 580 SXC

21 CD on Murrel Harvest at Kaliyavoor Fish Farm. 600 580 SXC

22 CD on Murrel Fillet Preparation. 600 580 SXC

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47

23 CD on Murrel Value Addition (Murrel Briyani, Cutlet,

Fingerchips, Pickle).

600 545 SXC

24 CD on Murrel Harvest at Veeralaperunselvi Fish Farm. 600 575 SXC

25 CD on Murrel Fish Cookies. 100 75 SXC

26 CD on Mass Culture of Live Feed. 250 225 SXC

14. Meetings/Seminars/Trainings/Kisan Mela, etc. organized

S.

No.

Details of Meetings/Seminars/Trainings, etc. Duration

(From-To)

No. of

Personnel

Trained

Organizer

(Name &

Address)

1 Formulation and Development of feed for

Murrels

22nd

- 24th

Mar. 2009

30 SXC

2 Workshop on Breeding and Seed Production

Techniques for Murrels

16th

-18th

Mar. 2009

30 SXC

3 Workshop on Seed Production and Feed

Formulation of murrels

30th

Jun.

2009

50

SXC

4 Induced Breeding and rearing Murrel larvae 7th

-9th

Oct.

2009

40 SXC

5 Workshop on Seed Production and Feed

Formulation for Murrel Farming

2nd

- 3rd

Feb.

2010

40 SXC

6 Workshop on Murrel farming for the farmers of

Pudduchery

31st Oct.

2009

12 CIFA

7 Workshop on “Murrel feed preparation” for the

farmers of Tamil Nadu

19th

Nov.

2009

15

CIFA

8 Training on “Murrel Seed Production and

Culture”

22-24th

Feb.

2010

45 CIFA

9 Workshop on “Murrel Seed Production and

Culture”

4-6th

Mar.

2010

42 CIFA

10 Training on “Value addition: Flavours from

Murrel”

24-25th

Sep.

2010

20 CIFA

11 Awareness programme on „Murrel culture‟ at

Nankar Bhaira, Nalbari, Assam

21st Nov.

2010

21 CIFA

12 Murrel culture and rearing of murrel larvae and

development of feed formulation. Puthu

Aayakudi, Palani, Tamil Nadu.

17th

Apr.

2010

30

SXC

13 Seed Production and Murrel farming. INFISH

2010, NFDB, Hyderabad. Andhra Pradesh

10th

Jul.

2010

43 SXC

14 Seed production and murrel farming. Govt. Arts

College, Nandanam, Chennai, Tamil Nadu.

15th

Jul.

2010

43 SXC

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48

15 Prophylaxis and Prevention of microbial

diseases for Murrel Culture at CARE, SXC.

4th

– 5th

Aug. 2010

33 SXC

16 Seed Production and Murrel farming. Bharathiar

University, Coimbatore, Tamil Nadu.

19th

Aug.

2010

75

SXC

17 Training on Feed Formulation on Murrel culture 24th

-25th

Sep. 2010

20 SXC

1 Training for fish farmers and trainers. CIBA, RA

Puram, Chennai, Tamil Nadu

29th

– 30th

Sep. 2010

10

SXC

18 Training for fish farmers and trainers. Matsya

Bhavan, Department of Fisheries, NFDB,

Hyderabad.

26th

Oct.

2010

10

SXC

19 Training for fish farmers and trainers. Inland

Fisheries Division, Bangalore.

18th

Nov.

2010

17

SXC

20 Training for fish farmers and trainers. St.

Xavier‟s College, Palayamkottai, Tamil Nadu.

14th

- 15th

Dec. 2010

26 SXC

21 Awareness programme on „Popularization of

murrel culture and other economical species in

Assam‟

11th

Sep.

2011

49 CIFA

22 One day Workshop on „BMP for murrel and

other economical species in Assam‟ at Nalbari.

12th

Sep.

2011

56 CIFA

23 Training on „ Murrel culture and its

popularization‟

7-9th

Feb.

2012

53 CIFA

24 Seed Production, Feed Formulation and Culture

of Murrels

22nd

Sep.

2011

30 SXC

25 Seed Production and Culture of Murrels 23rd

Nov.

2011

25 SXC

26 Commercial Murrel Culture 10th

Dec.

2011

68 SXC

27 Murrel Fish Food Expo 2012 7th

-8th

Jan.

2012

SXC

28 Workshop on Commercial Murrel Culture 9th

Feb.

2012

29 SXC

29 Workshop on Live and Formulated

F feed for Murrels

29th

– 30th

Mar., 2012

100 TNC

30 Nellai Fish Food Festival- 2013 7th

-9th

Feb

2013

SXC

31 Nanjil Fish Food Festival - 2013 16th

Mar.

2013

SXC

32 Training on „Breeding, seed production and

culture of murrel‟

5-7th

Sep.

2013

15 CIFA

33 Workshop on Murrel Culture and Value Added

Products

12th

Sep.

2013

42 SXC

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15. Participation in Conference/ Meetings/Trainings/ Radio talks, etc.

S.

No

Details of Meetings/Seminars/

Trainings/Radio talk, etc.(Name &Address)

Duration

(From-To)

Participant

(Name & Address)

Participation in International Conferences held Abroad

1 World Aquaculture 2010 at San Diego,

California

1-5 March 2010 Dr.M.A.Haniffa,

(As Resource Person)

SXC

2 Aquaculture America 2011 - Fin Fish Nutrition

session held at New Orleans Marriott, New

Orleans, Louisiana.

28 February – 3

March 2011

Dr.M.A.Haniffa,

(As Resource Person)

SXC

3 AQUA 2012 at Czech Republic

1-5 September

2012

Dr.M.A.Haniffa

( Two talks as

Resource Person)

SXC

Participation in National Conferences

1 National Meet on “Technological Innovations

in Agriculture” NASC complex, New Delhi

21-22 May 2010 Dr.M.A.Haniffa, SXC

Dr. Rajesh Kumar,

CIFA

2 TV talk on “murrel culture” during a

GRAMSAT programme on “diversification in

pisciculture” organized by Directorate of

Fisheries Orissa, Cuttack

27 August 2010 Dr. Kuldeep Kumar,

CIFA

3 Awareness program on “Murrel Culture” at

Nalbari, Assam

21 November

2010

Dr. Kuldeep Kumar,

CIFA

4 CAC meeting at St. Xavier College,

Palayamkottai, Tamil Nadu

13-14 December

2010

Dr. Rajesh Kumar,

CIFA

5 Asian Pacific Aquaculture 2011 Kochi, India. 17-21 January

2011

Dr. Rajesh Kumar,

Dr. Minakshi Sahoo,

CIFA

6 Bioved Research Society meeting, Allahabad,

U.P

19-20 February

2011

Dr. Kuldeep Kumar,

Dr. Minakshi Sahoo,

CIFA

7 Annual review meeting at UAS, Dharwad 15-16 March

2011

Dr. A. K. Sahu,

CIFA

8 Meeting on „Gender dimension for manpower

planning in Agriculture‟ at Directorate of

Research on Women in Agriculture,

Bhubaneswar

8 July 2011

Dr. U.L. Mohanty

CIFA

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50

9 National Workshop on „Strategies on

enhancement of productivity of IMC and

species diversification with special reference to

Magur, Murrel, Chitala, Pabda, Anabas,

Mahaseer and others in North-Eastern states‟.

Organized by NFDB, Ministry of Agriculture

Govt. of India at Guwahati.

8-9 July 2011

Dr. Kuldeep Kumar,

CIFA (delivered an

invited lecture on

„Prospects of murrel

culture in North East‟)

10 Workshop on „National Consultation on Gender

Perspective in Agriculture‟ at NASC Complex,

New Delhi

8-9 August, 2011

Dr. U.L. Mohanty

11 International Brainstorming Meet 2011 on

„Recent Advances In Fish Reproductive

Physiology‟ CIFA, Bhubaneswar, India.

16 August 2011.

Dr. Rajesh Kumar

Dr. U. L. Mohanty

CIFA

12 National Seminar on „Sustaining Agricultural

Productivity and food Security under Changing

Climate‟ at Google Research Centre,

Bhubaneswar

11-12 November

2011

Dr. U.L. Mohanty

CIFA

13 Training program on „Murrel for farmers‟ at

Guwahati, Assam

20-21 December

2011

Dr. Kuldeep Kumar,

CIFA

14 99th

Indian Science Congress at KIIT

University, Bhubaneswar

3-7 January 2012 Dr. Rajesh Kumar

Dr. U.L. Mohanty

Dr. M. Sahoo

Ms A. K. Mohanty

CIFA

15 14th

Indian Agricultural Scientists & Farmers‟

Congress organized by Bioved Research

Society, Allahabad, U.P

18-19 Feb 2012 Dr. Rajesh Kumar

Dr. M. Sahoo

CIFA

16 Technologies and Programmes for Second

Green Revolution in Eastern India at CRRI,

Cuttack

21-23 February,

2012

Dr. Rajesh Kumar

CIFA

17 National Conference on “Aquaculture: Fish for

Billion” at CIFA, Bhubaneswar

16-17 March

2012

Dr. Kuldeep Kumar

Dr. Rajesh Kumar

Dr. U.L. Mohanty

CIFA

18 National Workshop on “Gender in Fisheries: A

Future Roadmap”

CIFT, KOCHI, Kerala

4 April, 2012 Dr. U.L. Mohanty

CIFA

19 Workshop cum interactive meeting and training

camp with a view to offer an interactive

platform for the resource dependent

communities, at Kokrajhar, Assam

21-25 April 2012 Dr. Kuldeep Kumar

Dr. Rajesh Kumar

CIFA

20 National training on solid fermentation 29 May 2012 Dr. A. K. Sahu

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51

Dr. Kuldeep Kumar

Dr. Rajesh Kumar

Dr. U. L. Mohanty

CIFA

21 Training programme on “Fish breeding and

seed production” organized by Department of

Fisheries, Govt. of Odisha and KVK- Khurda,

Central Institute of Freshwater Aquaculture

17 September –

16 October 2012

Dr. Kuldeep Kumar

Dr. Rajesh Kumar

Dr. U. L. Mohanty

(As Resource Person)

CIFA

22 International symposium on „Genomics in

Aquaculture‟, CIFA, Bhubaneswar

22-23 January

2013

Dr. A. K. Sahu

Dr. Kuldeep Kumar

Dr. Rajesh Kumar

Dr. U. L. Mohanty

Dr. M Sahoo

Ms A K Mohanty

CIFA

23 XI AGRICULTURAL SCIENCE

CONGRESS on “Agricultural Education –

Shaping India‟s Future” OUAT, Bhuabneswar

7-9 February

2013

Dr. Rajesh Kumar

Dr. U. L. Mohanty

CIFA

24 Training Programme on “Competency

enhancement Programme for Technical Officers

of ICAR Institutes” at NAARM

13-22 May, 2013 Dr. U. L. Mohanty

CIFA

25 Consultation Workshop on „Development of

action plan for livelihood options‟ at Bali Island

of Sunderban

2 October 2013 Dr. Rajesh Kumar

CIFA

26 A talk on “Meeninam” during “Uzhavar

Uzhalagam” programme in All India Radio

23 June 2010 Dr. M. A. Haniffa,

SXC

27 Importance of zooplanktons in aquaculture and

effects of thermal and chemical stress on

zooplanktons, in National seminar on Recent

Trends in environmental Toxicology , BEED,

Maharashtra

24 July, 2010 Dr. K Altaff, TNC

28 A Radio talk on “Veralmeen Valarppu” on

Popularization of Murrel Culture in All India

Radio

31 August 2010 Dr. M. A. Haniffa,

SXC

29 Asian Pacific Aquaculture 2011 Kochi, India 17-21 January

2011

Dr. M. A. Haniffa,

SXC

30 Asian Pacific Aquaculture 2011 Kochi, India 17-21 January

2011

Mr. D. Manikandaraja,

SXC

31 Asian Pacific Aquaculture 2011 Kochi, India 17-21 January

2011

Mr.Bilal Ahmad

Paray,

SXC

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52

32 Importance of live feed in aquaculture fin fish

and shell fish aquaculture in India and feeding

strategies in Proceedings of national seminar on

emerging trends and challenges in Aquatic

Biotechnology held at Adirampatnam,

Thanjavur

11-12 February

2011

Dr. K Altaff, TNC

33 Mathsya Mela 2011 Karnataka, Bengaluru. 18 – 21February

2011

Mr. Hameedullah

Sherief

Dr. S. Velmurugan,

Mr. Manikandaraja,

SXC

34 One day Refresher training on Procurement and

Financial Management at NIANP, Adugodi,

Bangalore.

21 February 2011 Mr. Hameedullah

Sherief,

Mr. Manikandaraja,

SXC

35 Madurai Symposium 2011 on “Advancing

Development Knowledge towards sustainable

livelihoods

16 September

2011

Mr. S. Hameedullah

Sherief,

Mr.D.Manikandaraja

SXC

36 Chennai Aquaculture Technology Meet,

Fisheries Research and Extension, TANUVAS,

Chennai, Tamilnadu

16 – 17

November 2011

Mr. Bilal Ahmed

Paray,

SXC

37 Rastryiya Krishimela 2011 held at University of

Agricultural Sciences, GKVK Campus,

Bangalore

16 – 20

November 2011

Mr. S. Hameedullah

Sherief,

Mr.D.Manikandaraja

SXC

38 National Conference on Biotechnological

Approaches in Aquaculture, Bharathiar

University, Coimbatore, Tamilnadu

1 – 3 February

2012

Dr.M.A.Haniffa,

(As Resource Person)

Mr.S.Hameedullah

Sherief,

Mr.D. Manikandaraja,

Ms.K. Kavitha,

Ms.P.Jeya Sheela

SXC

39 Workshop on advances in aquaculture

techniques (WAAT), Bharathidasan University

Tiruchirappalli-620 024

20 – 24 February

2012

Mr. Mehraj ud din war

TNC,

40 Indian Science Congress Association Meet,

Regional Science Congress meet, Coimbatore

Chapter, Tamil Nadu

15 - 16 December

2012

Dr.M.A.Haniffa

(As Resource Person)

Mr.Bilal Ahmad Paray

Ms. P.Jeya Sheela,

SXC

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53

41 12th

Implementation Support Mission (ISM) of

the World Bank, TNAU, Coimbatore

22- 25 January

2012

Dr.M.A.Haniffa,

SXC

42 Biodiversity and Food security at Arulanandar

College, Karumathur

21- 22 February,

2013

Dr.M.A.Haniffa

(As Resource Person)

SXC

43 Intellectual Property Rights- Awareness, Tamil

Nadu

13 September

2013

Mrs. P.Jeya Sheela

SXC

44 Fish Feed Formulation at Tuticorin Fisheries

University, Tuticorin

22 October 2013 Mrs. P.Jeya Sheela

SXC

45 A lead talk in „National Symposium on

Biotechnology for Sustainable Community

Livelihood (BSCL 2014)‟ at Annai Velankanni

College, Tamil Nadu

23-24 January

2014

Mr.D.Manikandaraja

(delivered a lead talk

on „Aquaculture

Techniques for Murrel

Culture‟

SXC

16. Foreign Trainings/ Undertaken (National/ International) : Not Sanctioned

17. Performance Indicators (from inception to completion)

S.

No. Indicator Total No.

1. No. of production technologies released and/or adopted 7

2. No. of processing technologies released and/or adopted 2

3. Number of technologies/products commercialized based on NAIP

research 1

4. No. of new rural industries/enterprises established/ upgraded Nil

5. No. of product groups for which quality grades developed and agreed Nil

6. Total no. of private sector organizations (including NGOs)

participating in consortium 11

7. No. of farmers involved in consortia activities 52 (Tamil Nadu)+

86(Orissa) = 138

8. Total number of farmers‟ group developed for marketing and

processing 2

9. Number of patent/intellectual property protection applications filed

based on NAIP research 2

10. Number of patents/intellectual property protections granted/published

based on NAIP research Nil

11. Number of scientists trained overseas in the frontier areas of science Nil

12. Number of scientists trained overseas in consortium-based subject area Nil

13. No. of scientists participated in conference/seminar etc. abroad 1

14. No. of training organized/ farmers trained

Trai

ning

No.

Farmers

No.

1119

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54

33

15. Success stories 16 (Refer

Annexure IV)

16. Incremental employment generated (person days/year/HH)

Baseli

ne Final

17. Increase in income of participating households (` per annum)

Baseli

ne Final

18. Number of novel tools/protocols/methodologies developed 9

19. Publications

Articles in NAAS rated journals 7

Articles in other journals 16

Book(s) 4

Book chapter(s) 8

Thesis 11

Popular article(s) (English) 14

Newspaper article(s) 40

Seminar/Symposium/Conference/Workshop Proceedings 11

Technical bulletin(s) 2

Manual(s) 6

CDs/Videos 25

Popular article(s) in other language 9

Folder/Leaflet/Handout -

Report(s) -

18. Employment Generation (man-days/year)

S. No. Type of Employment Generation Employment

Generation

up to End of

Sub-project

Responsible

Partner

1. Seed production and Murrel farming by farmers

(entrepreneurship/self-employment)

3,139 CIFA,

Bhubaneswar

2. Engagement of manpower (workman) for murrel

farming (man-days/year)

11,680 CIFA,

Bhubaneswar

19. Assets Generated

(Details to be given on equipments and works undertaken in the sub-project, costing more

than ` 10,000/- in each case)

(i) Equipment/ Vehicles/ Research Facilities

S.No. Name of the equipment with

manufacturers name, model and Sr.No.

Year of

purchase

Quantity

(Nos.)

Total cost (in

lakhs)

Responsible

consortium

1 Fiber tank 2009 5 3,41,250.00 SXC

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55

2 Electronic balance (Shimadzu Japan

AX220)

2009 1 85,280.00 SXC

3 Computers (HCL) 2009 1 49,800.00 SXC

4 Feed pelletizer 2009 1 8,57,428.00 SXC

5 Ozone generator 2009 1 4,40,875.00 SXC

6 Oxygen cylinder (BTYPE) 2010 2 11,648.00 SXC

7 Research Microscope (Olympus: CH20i) 2009 1 1,87,200.00 SXC

8 Pipettes 2009 20 60,320.00 SXC

9 Digital camera (Nikon D 3000) 2010 1 30,300.00 SXC

10 Laminor Airflow (New Lab ) 2009 1 57,660.00 SXC

11 Xerox Machine (Canon Digital Copier : iR

2018N)

2009 1 1,07,501.00 SXC

12 Deep Freezer (DFS 290) 2009 1 1,82,000.00 SXC

13 Fax machine (CANON 4350D) 2009 1 18,900.00 SXC

14 Refrigerators (GL 278VA4,

GL211SAG4)

2009 2 27,400.00 SXC

15 Diesel generator 2009 1 2,89,000.00 SXC

16 Air Conditioner (ONIDA 3.0 ton) 2009 1 31,500.00 SXC

17 LCD with Laptop (Sony: VPL – EX50 ) 2009 1 64,990.00 SXC

18 Monoblock motors (TEXMO) 2009 4 41,207.00 SXC

19 Binocular Microscope (Olympus: CH20i) 2009 1 41,800.00 SXC

20 Statistical software (Image analysis

software)

2009 1 40,360.00 SXC

21 Auto clave (EQUITRON) 2010 1 97,240.00 SXC

22 KG Balance (ECB 300, ECB 3000) 2009 2 8,320.00 SXC

23 Hot air oven 2009 1 62,080.00 SXC

24 Refrigerated centrifuge (REMI C 24B1) 2009 1 1,66,348.00 SXC

25 Water Bath (S.S. Digital) 2009 1 14,137.50 SXC

26 Fencing net 2009 160 m 92,000.00 SXC

27 Digital PH meter (ELiCO model no. LI

127)

2009 1 6,340.00 SXC

28 Digital DO analyser (831E DO meter) 2009 1 5,710.00 SXC

29 Microtome (PICOASI) 2009 1 19,406.00 SXC

30 Salanometer ATAGO) 2009 1 10,688.00 SXC

31 Submersible motor & Accessories

(DECCAN )

2009 3 1,67,400.00 SXC

32 Video camera (HDR-XR 100 E) 2009 1 49,000.00 SXC

33 Fishing nets 2009 20 80,774.00 SXC

34 C-Mount 0.7X Relay lens (Cat no.

MBB73520)

2009 1 38,590.00 SXC

35. Microscope image projection system 2009 1 40,512 SXC

36 Microscope with laptop

(Olympus trinocular microscope, model

CX31 with HP Pavillion)

2009-10 1 2,89,868 CIFA

37 Deep freezer (Voltas Deep Freezer, 2009-10 2 58, 000 CIFA

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56

Model- 500 Ltr, D. D. Metal top)

38 Camera (Nikon camera Model : P 80

coolpix)

2009-10 1 22,500 CIFA

39 Meat mill (Local product) 2009-10 1 10,169 CIFA

40 FRP Tanks (M. R. Aqua Tech.)

2013-14 10 (500

L)

15 (200

L)

2,04,850 CIFA

41 Honda Brush Cutter, Model- UMK 435 T 2013-14 1 26,675 CIFA

42 Electronic Balance,

Model- Citizen CX 220

2013-14 1 78,750 CIFA

43 pH Meter 2013-14 1 33,667 CIFA

44 DO meter 2013-14 1 1,07,825 CIFA

45 Refractometer 2013-14 1 43,130 CIFA

46 Tissue homoginiser

Model. RQT-124/A,

2013-14 1 15,890 CIFA

47 Water-bath with shaker

Model.- BDI-67

2013-14 1 66,965 CIFA

48 Water Purifier,

Model- AQUELIX 5

2013-14 1 2,61,050 CIFA

49 Cooling Centrifuge

Remi, India

2013-14 1 3,29,718 CIFA

50 Refrigerator (Vestfrost) 2013-14 2 1,32,296 CIFA

51 Kirloskar 10 KVA silent DG Set. 2013-14 1 3,37,000 CIFA

52 Diesel pumps (5hp & 4hp), Kirlosker. 2013-14 1 5hp

1 4hp

94,500 CIFA

53 Inverter (common name- SRC 3000UXI)

Model No. APC smart-UPS RC 3000VA

230v

2013-14 1 89,460 CIFA

54 Air Blower

Model No.- GP7500, Model No.-GP3000

2013-14 1 5,66,365 CIFA

55 Feed pelletizer, Fabricated 2010-11 1 8,21,520.00 TNC

ii) Research Facilities: Books

S. No. Name of the Research facilities and

Address of Agency

Year of

Purchase

Quantity

(Nos.)

Total

cost (`in

Rs.)

Responsibl

e Partner

1 M/S Narendra Publishing House, 141

Kishan Dutt Street, Maliwara-110 006

2009 11 14000 SXC

2 M/S, Daya Publishing House, Publishers

and Distributors, 4760- 61/23, Ansari

Road, Darya Ganj, New Delhi-110002

2009 85 70537 SXC

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57

3 M/S Narendra Publishing House, 141

Kishan Dutt Street, Maliwara-110 006

2009 12 60,657 CIFA

4 M/S Narendra Publishing House, 141

Kishan Dutt Street, Maliwara-110 006

2009 21 22924 SXC

5 M/S, Daya Publishing House, Publishers

and Distributors, 4760- 61/23, Ansari

Road, Darya Ganj, New Delhi-110002

2009 12 14799 SXC

6 M/s. Abishek Book Agency

Block A, Plot No 49/A, Second Floor,

Krishan Vihar, Delhi-110086

2010 23 18204 SXC

7 M/s. A & A Book House, T.C-14/1130

(1), ST.George Lane, Kumarapuram

Medical College Post, Trivandrum-

695011

2010 49 36869 SXC

8 M/s. Agrobios, Behind Nasrani Cinema,

Chopasani road, Jodhpur- 342002

2010 6 3663 SXC

9 M/s. Sri Ram Book Agency, 69, P.V

Ammal Street, West Mambalam,

Chennai- 600033

2010 2 1300 SXC

10 M/S. Eagle Book Centre, Opposite VOC

Ground, 168, Trivandrum Road,

Palayamkottai- 627002

2010 24 9464 SXC

11 M/S. Velavan Book Centre, 104- A,

Trivandrum Road, Opposite VOC

Ground, Palayamkottai

2010 4 35000 SXC

12 M/s. Abishek Book Agency

Block A, Plot No 49/A, Second Floor,

Krishan Vihar, Delhi-110086

2011 12 80658 SXC

13 M/S. Meena Enterprises, H-250,

Housing Board, Ellis Nagar, Madurai -

625010

2011 11 9560 SXC

14 M/S. Velavan Book Centre, 104- A,

Trivandrum Road, Opposite VOC

Ground, Palayamkottai

2011 5 23499 SXC

15 M/S. Power Book House, Old No:13,

New No: 12, Kalki Nagar Main Road,

Arumbakkam, Chennai

2011 19 24214 SXC

16 M/S. Eagle Book Centre, Opposite VOC

Ground, 168, Trivandrum Road,

Palayamkottai- 627002

2011 7 2630 SXC

17 M/S Agrosciences Book Centre, 19B,

Daryaganj, ansary Road, NEW Delhi-

110 002

2010 17 62,370 CIFA

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18 M/S Narendra Publishing House, 141

Kishan Dutt Street, Maliwara-110 006

2011 8 42,860 CIFA

19 M/s. New India Publishing Agency,

101, Vikas Surya Plaza, CU Block,

L.S.C. Mkt, Pitampura, New Delhi-

110088

2011 5 6444 SXC

20 M/s. Abhishek Book Agency,

Block A, Plot No 49/A, Second Floor,

Krishan Vihar, Delhi-110086

2012 32 125486 SXC

21 M/S Books Media,

A 3/53, Paschim Vihar, New Delhi

110063

2012 24 62,544 CIFA

(iii)Works

S.No. Particulars of the work, name and

address of agency awarded the

work and site of construction

Year of

work

done

Quantity

(Nos.)

Total cost

(Rs.)

Responsible

consortium

1. Earthern Ponds

M/S S.Kamaraj

Building Contractor

57,Senthil Nagar

Palayamkottai

2009 4 nos. (7m x

3m x 1.5 m

each)

2,31,850 SXC

2. Cement Tanks

M/S Muthu Construction

No,23 A Kottur Road

Palayamkottai

2010 12 nos.

(1.5m x

1.0m x 1.0m

each)

1,10,820 SXC

3. Moina unit

M/S Muthu Construction

No,23 A Kottur Road

Palayamkottai

2010 6 nos. (5m x

5m x 2m

each)

2,27,974.00 SXC

4. Larval Rearing Unit I

M/S New Bharath Builders

92/18A/2, Nethaji Road

Melapalayam

2010 10.75m x

10.10m x

4.3m

9,57,700 SXC

5. Larval Rearing Unit II

M/S A.Mohamed Nowfal

&M/S St.George Engineering

Works& M/S AKR Fabricators

Palayamkottai-627002.

2010 1.75m x

5.5m x 2.5m

4,29,038 SXC

6. Seed Production and Larviculture

Infrastructure

M/S Asian Analytical Laboratories

2010 18 Wooden

Stands,

135 Glass

26,06,560 SXC

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Pvt .Ltd

4/2 Bharathiyar Street

ECR,Palavakkam, Chennai-41.

Tanks,

6 Filteration

units

7. Laterite lining of two ponds M/S K.

C. Mohanty

Plot No. S/2, Indira Gandhi Housing

Board Colony, Sisupalgarh, BBSR

2009 2 2,49,992 CIFA

8. Renovation of old facilities into feed &

store room by M/S Dhurandhar Parida

C/o Nayak Medical Store, Uttara, P.O.

Kausalyaganga, BBSR-2

2009 1 49,004

CIFA

9. Construction of cement cisterns for larval

rearing by M/S K. C. Mohanty Plot No.

S/2, IndiraGandhi Housing Board

Colony, Sisupalgarh, BBSR

2009 8 (5x3 m2) 4,98,814 CIFA

10. Construction of hatchery shed for

larval rearing by M/S Parsuram

Biswal

Uttara, Near Petrol Pump,

Kausalyaganga, BBSR-2

2009 1 4,78,426 CIFA

11. Construction of small cement

cisterns (2 x 1 m2) for larval rearing

by M/S K. C. Mohanty, Plot No. S/2,

Indira Gandhi Housing Board

Colony, Sisupalgarh, BBSR

2010 12 (2x1 m2) 3,89,287 CIFA

11. Construction of Hatchery, bore well,

drain, pipeline etc. by M/S Parsuram

Biswal, Uttara, Near Petrol Pump,

Kausalyaganga, BBSR-2

2010 1

4,98,046

CIFA

12. Renovation of pond by laterite

lining

Making of cement tank (2 x 1 m2),

Pipeline arrangement

by M/S K. C. Mohanty Plot No.

S/2, Indira Gandhi Housing Board

Colony, Sisupalgarh, BBSR

2010 1

7

2,66,884 CIFA

13 Electrification of hatchery

C/O. Subas Behera

Qrs. No. 2R-II(P), Unit 1,Ashok

Nagar, BBSR-9

2010 20,176 CIFA

(iv) Livestock

(Details of livestock procured/produced in the sub-project)

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S. No. Details of Livestock

(Breed, etc.)

Year of

Procurement/

Production

Nos. Total

Cost

Responsible

Partner

1 Nil NA NA NA NA

(v) Revenue Generated

(Details may be given on revenue generated in the sub-project viz., sale of seeds, farm

produce, products, patents, commercialization, training, etc.)

S. No. Source of Revenue Year Total amount

(`)

Responsible

Partner

1. By supply of Murrel Fingerlings 2009 -

2010

5000 Lead Consortium –

CARE,SXC

20. Awards and Recognitions

S.

No.

Name,

Designation,

Address of

the Person

Award/

Recognition

(with Date)

Institution/ Society

Facilitating (Name &

Address)

Responsible

Partner

1 Dr Kuldeep Kumar,

Principal Scientist, CIFA,

Bhubaneswar-2

Scientist of the

year 2009

BIOVED Research Institute

of Agriculture and

Technology, Allahabad, UP

CIFA

2 Dr.M.A.Haniffa, CARE,

St. Xavier‟s College,

Palayamkottai

Best

Appreciation

Certificate, 2010

ICAR, New Delhi SXC

3 Dr.M.A.Haniffa, CARE,

St. Xavier‟s College,

Palayamkottai

Received

memento, 2010

INFISH 2010, NFDB,

Hyderabad SXC

4 Dr.M.A.Haniffa, CARE,

St. Xavier‟s College,

Palayamkottai

Received

memento for

best Recipe,

2010

INFISH 2010, NFDB,

Hyderabad SXC

5 Mr. S.Hameedullah Sherief

– RA, CARE, St. Xavier‟s

College

Received

memento, 2011 Mathsya Mela Karnataka SXC

6

Mr. S.Hameedullah Sherief

– RA, CARE, St. Xavier‟s

College

Received second

prize for fish

recipe

competition,

2011

Mathsya Mela Karnataka SXC

7 D.Manikandaraja SRF,

CARE, St. Xavier‟s

College

Received Award

for best exhibits,

2011

Mathsya Mela Karnataka SXC

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8 Dr.M.A.Haniffa, CARE,

St. Xavier‟s College,

Palayamkottai

A memento,

2011

DHAN Vayalagam (Tank)

Foundation, Madurai,

Tamilnadu

SXC

9 Dr Rajesh Kumar (one of

the team member),

Scientist, CIFA,

Bhubaneswar-2

Krushakabandhu

Team Award

2011

Orissa Krushak Samaj,

Bhubaneswar, Orissa. CIFA

10 Dr Rajesh Kumar

Scientist, CIFA,

Bhubaneswar-2

Young Scientist

Award, 2012

BIOVED Research Institute

of Agriculture and

Technology, Allahabad, UP

CIFA

11

Dr.(Mrs.) Minakshi Sahoo,

SRF, CIFA, Bhubaneswar-

2

Young Scientist

Award, 2011

BIOVED Research Institute

of Agriculture and

Technology, Allahabad, UP

CIFA

12 Dr.(Mrs) Minakshi Sahoo ,

SRF, CIFA, Bhubaneswar-

2

Distinguished

service award

2012

BIOVED Research Institute

of Agriculture and

Technology, Allahabad, UP

CIFA

21. Steps Undertaken for Post NAIP Sustainability

Linkages have been developed with reputed laboratories / organizations for various activities:

S.No. Studies / Activities Laboratories / Organizations

1. Medicinal value of Murrels Manian laboratories, Coimbatore;

Antartica College of Pharmacy, Tirunelveli;

Changethu Ayurvedic Hospital and Research

Centre, Kerala

2. Commercialization of

Murrel Value Added

Products

Millennium Exports, Chennai;

Arasan Super Market, Tirunelveli;

Shrimpex Aqua House, Cochin

3. Marketing of Live Murrels Fish Vendors from Tirunelveli, Tenkasi,

Virudhunagar, Madurai, Coimbatore, Chennai,

Thrissur, Cochin and Hyderabad

CARE has a network of 1119 fish farmers in Tamil Nadu, Kerala, Andhra Pradesh, and

Punjab

All activities are updated in our website www.caresxc.org.

Most of the video CDs are available in Youtube (as Murrel Culture)

Regular training and workshop are conducted.

Linkages established with Tirunelveli Corporation, District Collectorate of Tirunelveli,

FFDA, KVKs, NFDB, SHGs, NGOs and State Fisheries Colleges, State Fisheries

Departments (Bangalore, Hyderabad, Chennai).

Established network of fish vendors from Tirunelveli, Tenkasi, Virudhunagar, Madurai,

Coimbatore, Chennai, Thrissur, Cochin and Hyderabad.

Awareness on popularization by media via. Newspapers, technical manuals, Radio Talks, TV

Programmes, etc.

Centre for Aquaculture Research and Extension (CART) established in September 2013 at

KIFVI, Kalakad Village for regular training and workshop.

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CARE as a liaison between stakeholders and fish farmers for seed supply.

Liaison between fish farmers and fish vendors for supply of table size murrels

22. Possible Future Line of Work

(Comments/suggestions of CPI regarding possible future line of work that may be taken up

arising out of this sub-project)

1. Establishment of permanent hatchery at Centre for Aqua culture Research and Training

(CART), Kings Farm Ville, established in September 2013 at Kalakkad Village, to supply

murrel seeds to farmers throughout the year.

2. Popularisation of medicinal value of murrels especially for treatment of wound, arthritis and

convalescence by establishing tie up with Siddha and Ayurvedic Hospitals.

3. Studies on molecular characterization and gene expression of aetiological agent with regard

to common occurrence of EUS in murrels.

4. There is a common psychology that murrels are highly predatory. But our experience shows

murrel compatability with other species. Our future studies include murrel breeding

behaviour, parental care, cannabolistic behaviour and compatability with other fishes etc, for

which only few reports are available.

5. To overcome the loss due to death of murrels, fish vendors have to be trained for the

preparation of fillet from dead murrels as well as safe transport of live murrels from the

harvested area to markets.

6. Popularization has yet to be made with regard to cold storage of fillet and its usage for the

preparation of ready to eat and ready to cook products among SHGs, Housewives, and

Officegoers.

7. Future Studies on Biodiversity status of different murrels (edible and ornamental) from

ponds, lakes, rivers, and reservoirs of different states of India are necessary.

8. Conservation of threatened murrel species by seed production and river ranching attempts is

yet to be made in India.

23. Personnel

(Staff of Lead Centre & Partner-wise, their Name, Designation, Discipline and Duration)

From – To (DD/MM/YYYY)

Research Management (CL)

1. Rev.Dr.S.Alphonse Manickam S.J. , Principal 01.11. 2008- 31.5. 2012

2. Rev.Dr.A.Joseph S.J., Principal 01.06. 2012- 31.05.2013

3. Rev.Dr.V.Gilburt Camillus S.J., Principal 01.06.2013 – 31.03.2014

Scientific (CPI, CCPI, others)

4. Dr.M.A.Haniffa, CPI 01.11.2008 – 31.3.2014

5. Dr.A.John De Britto, CCPI 01.11.2008 – 31.3 2009

6. Dr.K.Altaff, CCPI 01.11.2008 – 31.3. 2012

7. Dr. A.K. Sahu, CCPI 01.10.2008 - 30.09.2013

8. Dr. Kuldeep Kumar, CCPI 01.10.2008 - 31.03.2013

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63

9. Dr. Rajesh Kumar, CCPI 01.10.2008 - till date

Technical (CPI, CCPI, others)

10. Dr. (Mrs.) U.L. Mohanty, CIFA 01.10.2008 to till date

11. Mr.A.Alagianambi, SXC 01.11.2008 – 31.07.2009

12. Mrs.S.Lakshmi Priya, SXC 10.08.2009 – 31.3.2014

Contractual (CPI, CCPI, others)

13. Mr.M.Dhanaraj, SXC 01.11.2008 – 01.05.2010

14. Mr.C.Muthuramakrishnan, SXC 01.11.2008 – 01.05.2010

15. Mr.S.V.Arun Singh, SXC 01.11.2008 – 01.05.2010

16. Mr.D.Manikandaraja, SXC 01.11.2008 – 31.10.2012

08.03.2013 – till date

17. Mr.Bilal Ahmad Paray, SXC 01.11.2008- 31.05.2010

01.08.2010 – 31.03.2011

01.07.2012 – till date

18. Mr.C.Palpandi, SXC 01.06.2010 – 31.07.2010

19. Mr.S.Hameedullah Sherief, SXC 01.06.2010 – 31.03.2012

20. Mr.S.Sankar Ram, SXC 01.06.2010 – 31.07.2010

21. Miss.P.Shanthi, SXC 01.06.2010- 31.10.2011

22. Mr.S.Velmurugan, SXC 03.08.2010 – 31.03.2011

23. Miss.K.Kavitha, SXC 01.04.2011 – 31.03.2012

01.11.2012 – till date

24. Miss.K.Lavanya, SXC 01.04.2011 – 31.10.2011

25. Miss.P.Jeya Sheela, SXC 01.11.2011 – 31.03.2012

01.06.2012 – till date

26. Dr.R.Arthimanju, SXC 01.11.2011 – 31.03.2012

27. Dr.M.James Milton, SXC 01.04.2012 – 07.03.2013

28. Dr.Ajaz Ali Bhat, SXC 01.04.2012 – 31.10.2012

29. Miss.B.Kavitha Bharathi, SXC 01.04.2012 – 30.06.2012

30. Miss.D.Viji Margret, SXC 01.04.2012 -30.06.2012

31. Mr.S.Ramesh, SXC 01.04.2012 – 30.06.2012

32. Mr.K.Venkatesh, SXC 01.11.2012 – 31.03.2013

33. D.Pushparani, SXC 01.04.2013 – till date

34. Ms. Noor Jahan, CIFA 20.01.2009 - 31.10.2011

35. Mr. D. Damle, CIFA 21.01.2009 - 13.11.2009

36. Dr. (Mrs.) Minakshi Sahoo, CIFA 05.12.2009 - till date

37. Ms. Amita Kumari Mohanty, CIFA 15.12.2011 - 15.05.2013

38. Mrs. Anjana Devi Sahu, CIFA 04.06.2013 - till date

39. Mr. Mehraj Ud Din War 01.11.2008 – 31.03.2012

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64

24. Governance, Management, Implementation and Coordination

A. Composition of the various committees (CIC, CAC, CMU, etc.)

S. No. Committee

Name

Chairman

(From-To)

Members

(From-To)

1. CIC

Dr.V.Sundararaj

(2008 – 2014)

19th

October 2010

1. Mr.T.Dharmaraj, Dean of Arts

2. Dr. A. Lourdusamy, Dean of Science

3. Mr.J.Carmel Rajan, Superintendent

14th

September 2011

1. 1.Dr.P.Lourdes Poobala Rayan, Dean of Arts

2. 2. Dr.A.Lourdusamy,

3. Dean of Science

4. 3. J.Carmel Rajan, Superintendent

10th

January 2013

1. Dr.P.Lourdes Poobala Rayan, Dean of Arts

2. Mr.J.Carmel Rajan, Superintendent

3. Dr.A.Lourdusamy,

Dean of Science

7th

March 2013

1. Dr.P.Lourdes Poobala Rayan, Dean of Arts

2. Mr.J.Carmel Rajan, Superintendent

3. Dr.A.Lourdu Samy, Dean of Science

2. CAC 11th May 2010

1. Dr.V.Sundararaj, Chairman,CAC

2. Dr.G.S.Vijayalakshmi, Member, CAC

3-5th

August 2010

1. Dr.V.Sundararaj, Chairman,CAC

2. Dr.G.S.Vijayalakshmi, Member, CAC

3. Mr.S.Sivasuryanarayanan, Member, CAC

4. Mr.S.Manikkaraj,Member, CAC

13th

December 2010

1. Dr.V.Sundararaj, Chairman,CAC

1. 2. Dr.G.S.Vijayalakshmi, Member, CAC

2. 3. Mr.S.Sivasuryanarayanan, Member, CAC

3. 4. Mr.S.Manikkaraj,Member, CAC

23rd

March 2011

1. 1. Dr.V.Sundararaj, Chairman,CAC

2. 2. Dr.G.S.Vijayalakshmi, Member, CAC

11th

August 2011

1. Dr.V.Sundararaj, Chairman,CAC

2. Dr.G.S.Vijayalakshmi, Member, CAC

3. 3. Mr.Phlip Abraham, Member, CAC

4. 4. Mr.H.Peer Mohamed Rafik,Member, CAC

18th

October 2011

3. 1. Dr.V.Sundararaj, Chairman,CAC

2. Dr.G.S.Vijayalakshmi, Member, CAC

9th

January 2013

1. Dr.V.Sundararaj, Chairman,CAC

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2. Dr.G.S.Vijayalakshmi, Member, CAC

4. 3. Mr.H.Mohammed Riyaz, Member, CAC

5. 4.Mr.S.Muthukumar, Member, CAC

8th

March 2013

1. Dr.V.Sundararaj, Chairman, CAC

2. Dr.G.S.Vijayalakshmi, Member, CAC

31st July 2013

1. Dr.V.Sundararaj, Chairman,CAC

2. Dr.G.S.Vijayalakshmi, Member, CAC

3. Mr.S.Sivasuryanarayanan, Member, CAC

4. Muruga Pandi, Member, CAC

7th

November 2013

1. Dr.V.Sundararaj, Chairman, CAC

2. Dr.G.S.Vijayalakshmi, Member, CAC

3. CMU 15th

September 2010

1. Dr.A.Dhananjayan, Head, Dept of Folklore

2. Dr.P.Devaraju, Head, Dept of Economics

3. Mr.J.Carmel Rajan, Superintendent

4. Dr.Dharmaraj, Dean of Arts

15th

September 2011

1. Dr.P.Devaraju, Head, Dept of Economics

2. Mr.J.Carmel Rajan, Superintendent

3. Dr.P.Lourdes Poobala Rayan, Dean of Arts

4. Dr.A.Dhananjayan, Head, Dept of Folklore

9th

January 2013

1. Dr.A.Dhananjayan, Head, Dept of Folklore

2. Mr.J.Carmel Rajan, Superintendent

6th

March 2013

5. Dr.S.V.L.Michael, Head, Dept of Economics

6. Mr.J.Carmel Rajan, Superintendent

7. Dr.P.Lourdes Poobala Rayan, Dean of Arts

3. Dr.A.Dhananjayan, Head, Dept of Folklore

B. List of Meetings organized (CIC, CAC, CMU, etc.)

S. No. Details of the meeting Date Place & Address (Where meeting

was organized)

1. CIC 19th

October 2010

CARE,

St.Xavier‟s College,

14th

September 2011

10th

January 2013

07th

Mach 2013

2. CAC 11th

May 2010

3-5th

August 2010

13th

December 2010

23rd

March 2011

11th

August 2011

18th

October 2011

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66

9th

January 2013 Palayamkottai.

8th

March 2013

31st July 2013

7th

November 2013

3. CMU 15th

September 2010

15th

September 2011

11th

January 2013

6th

March 2013

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Part-III: Budget and its Utilization

STATEMENT OF EXPENDITURE (Final) for St.Xavier‟s College (Lead Institute)

(Period from 01.11.2008 to 31.03.2013)

Sanction Letter No. F.No. 1(5)/2007- NAIP dt. August 22, 2008

Total Sub-project Cost: Rs. 454.76 lakhs

Sanctioned/Revised Sub-project cost (if applicable): Rs.317.41/- lakhs

Date of Commencement of Sub-project: 01.11.2008

Duration: From 01.11.2008 to 31.03.2014 (DD/MM/YYYY)

Funds Received in each year

I Year `Rs.10846000/-

II Year `Rs.3013941/-

III Year ` Rs.3602600/-

IV Year Rs.3452902/-

V Year Rs.3108316/-

Bank Interest received on fund (if any) ` Rs.403310/-

Total amount received ` Rs.240.75/- lakhs

Total expenditure ` Rs.238.67/- Lakhs

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PART-IV: DECLARATION

This is to certify that the final report of the Sub-project has been submitted in full

consultation with the consortium partners in accordance with the approved objectives and

technical programme and the relevant records, note books; materials are available for the same.

Place:_________

Date:_________ Signature of Consortium Principal Investigator

Signature & Date

Consortium Co-Principal Investigator

Signature & Date

Consortium Co-Principal Investigator

Signature & Date

Consortium Co-Principal Investigator

Signature & Date

Consortium Co-Principal Investigator

Comments & Signature of Consortium Leader

Date:

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Proforma -1

Validation of Developed/ Released/ Adopted Production Technologies/ Innovations

(Page limit: 3 pages/technology)

1. Title of the Sub-project: A Value Chain on Murrel Production in Tamil Nadu and Orissa

(F.No.1(5)/2007-NAIP dt. 22 August, 2008).

2. Name of CPI/CCPI:

CPI:Dr.M.A.Haniffa, St.Xavier‟s College (Autonomous), Palayamkottai, Tamil Nadu.

CCPIs:Dr.A.K.Sahu, Dr.Kuldeep Kumar & Dr.Rajesh Kumar,CIFA, Bhubaneswar.

3. Title of the Technology: Murrel Seed Production

4. Information on Existing Farming Systems, Practices, Productivity Levels and Income in

the Target Area:

At present, farmers who are culturing murrels buy seeds from fishermen who collect

them from the wild. Most farmers who are interested in murrel farming are unable to culture

murrels due to non availability of seeds. Murrel seed collection from the wild is unpredictable

due to non availability of murrel brooders as well as monsoon failure. Moreover, murrel farmers

are not familiar to seed production by induced breeding and/ or artificial fertilization. At this

juncture, through the present ICAR- NAIP subproject, 1119 farmers were trained in Tamilnadu

and Orissa for various seed production techniques associated with murrel farming.

Before implementation of NAIP subproject farmers collected seeds from the

wild/purchased seeds from fishermen and released them into village ponds/lakes during rainy

season (October-December). During summer (April-May) when ponds/lakes dried, they

harvested table size fish and sold them to markets. At present also, such practice is going on but

slowly, the murrel farmers are changing their attitude i.e., instead of purchasing the seeds from

middle men, they are producing their own seeds for farming.

5. Key Intervention(s) Introduced:

Succeeded in seed production of striped murrel Channa striatus and giant murrel

C. marulius in indoor farm and upscaling of seed production at stakeholders farms in

Tamilnadu and Orissa.

A breakthrough was made for year round availability of murrel fingerlings by induced

breeding for farming.

6. Results

Status of Dissemination/ Commercialization; and, Extent of Adoption and Success, If

Applicable; with Supporting Data (with Tables and Photographs as Annexure)

After implementation of NAIP subproject murrel seeds were produced as details given in

the table.

Table 1: Seed Production at CARE and CIFA

Years SXC, Tamil

Nadu

At CIFA,

Bhubaneswar

By

Stakeholders

Total

2009-10 11,361 41,000 - 52,361

2010-11 36,500 37,820 22,500 96,820

2011-12 60,028 46,938 35,000 1,41,966

2012-13 NA 75,000 15,000 90,000

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70

2013-14 NA 62,500 37,500 1,00,000

Total 1,07889 2,63,258 1,10,000 4,81,147

7. Brief Description of Technology for Release:

Murrel seed production:

Indian fish farmers are not familiar to murrel breeding techniques. Hence they depend on

fishermen for seed collection from the wild. Moreover, supply of seeds by fishermen is only

during southwest monsoon (June to August) and northeast monsoon (October to December)

Hence induced breeding technique is necessary, which is a boon to fish farmers for availability

of seeds throughout the year.

Research team of CARE and CIFA succeeded in induced breeding of striped murrel

Channa striatus and giant murrel Channa marulius using natural (HCG, pituitary hormone) and

synthetic hormones (LHRH, Ovaprim, Ovatide, Ova FH) by intramuscular injection to brooders

(each set: one male and two females) and after that they were released into breeding tanks (4.5m

x 4m x 2m). Based on the results, HCG was recommended to stakeholders for upscaling of

murrel seed production in their ponds. About 4,81,147 seeds were produced after implementation

of NAIP sub project. From a single breeding set of striped murrel, 8,000 – 12,000 eggs were

obtained. The eggs of striped murrel C.striatus hatched out within 12 – 16 hrs and the hatchlings

started taking exogenous feed from third day onwards. The growth and survival of hatchlings

depend much on the type of feed and hence live feed culture occupies an important role in murrel

farming to decide the success of farmer.

8. Expected Outcome/Impact of the Technology:

Production of fingerlings at stakeholders level (1,00,000/ year).

For large scale production of 1 lakh seeds - Brood fish maintenance pond (0.10 acre),

Breeding pond (0.25 acre), Nursery tanks (0.50 acre), Fry rearing tanks (0.65 acre), a land

of 1.5 acres are recommended.

Upscaling of seed production at stakeholders farms (1, 00,000 – 5, 00,000) according to the

availability of land and water resources.

Table 8 Expected Impact of Technology

For small scale production of 10,000 fingerlings, 8-9 hatcheries (each 3ft diameter with 3 ft

depth) are necessary requiring a total area of 300 sq ft. The expenditure involved Brood

Fish, Feed, Hormone, Plankton Culture Tanks, Hatcheries, etc and the net profit is

Rs.65,000/- per year for the production of 10,000 fingerlings in an area of 300 sq.ft.

(8.1) Expected Increase in Area, Production and Net Income

(8.2) Others Nil

Expected Area Number of seeds Net income (Rs.)

0.5 Acres 1 lakh seeds 3 lakhs

2.5 Acres 5 lakhs seeds 15 Lakhs

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71

9. Whether findings have been published? If so, give the citation and enclose copy of the

publication.

Research Articles:

Bilal Ahmad Paray, Haniffa M A and Manikandaraja D. 2012. Induced ovulation and

spawning of a striped snakehead murrel Channa striatus (Bloch) under captive conditions.

Journal of Research in Animal Sciences 1(1): 033-039.

Bilal Ahmad Paray, Haniffa M A, Manikandaraja D and James Milton M. 2013. Breeding

Behaviour and Parental Care of the Induced Bred Striped Murrel Channa striatus under

Captive Conditions. Turkish Journal of Fisheries and Aquatic Sciences 13: 707-711.

Bilal Ahamad Paray, Haniffa M A and Ananth Kumar Y. Studies on Embryonic and Larval

Development of Induced Bred Channa striatus. Envirobiotech Journal. (communicated)

Book Chapters:

Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) Ka beej utpadan ebm Palan

(Hindi) (Seed Productuction and culture of murrel) (in) Training manual on Jalkrishi mein

Dakhata Vikash, p 50-54. Published by Director, CIFA, Kausalyaganga, Bhubaneswar, India

on 15-19 July 2013.

Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) Ka beej utpadan ebm Palan

(Hindi) (Seed Productuction and culture of murrel) (in) Training manual on Unnat mithajal

matya palan, p 54-57. Published by Director, CIFA, Kausalyaganga, Bhubaneswar, India.

12-16 December 2013.

Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) ka beej utpadan ebm Palan

(Hindi) (Seed Productuction and culture of murrel) (in) Training manual on ‘Mithajal Matya

palan mein unnat praudhogiki’, p56-59. Published by Director, CIFA, Kausalyaganga,

Bhubaneswar, India. 20-24 December 2013.

Kumar K, Kumar R, Mohanty U L and Sahu A K. 2012. Murrel culture and its popularization.

(in) Training manual on Fish Breeding and Seed Production, p37-45, KVK, CIFA (Eds),

Department of Fisheries, Govt. of Odisha and KVK- Khurda, Central Institute of Freshwater

Aquaculture, Kausalyaganga, Bhubaneswar, India.

Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) Ka beej utpadan ebm Palan

(Hindi) (Seed Production and culture of murrel) (in) Training manual on Jalkrishi mein

Dakhata Vikash, p 50-54. Published by Director, CIFA, Kausalyaganga, Bhubaneswar, India

on 15-19 July 2013.

Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) Ka beej utpadan ebm Palan

(Hindi) (Seed Production and culture of murrel) (in) Training manual on Unnat mithajal

matya palan, p 54-57. Published by Director, CIFA, Kausalyaganga, Bhubaneswar, India.

12-16 December 2013.

Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) ka beej utpadan ebm Palan

(Hindi) (Seed Production and culture of murrel) (in) Training manual on ‘Mithajal Matya

palan mein unnat praudhogiki’, p56-59. Published by Director, CIFA, Kausalyaganga,

Bhubaneswar, India. 20-24 December 2013.

Popular Articles:

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72

Haniffa M A. 2009. ICAR NAIP sponsored workshop on breeding and seed production of

murrels. Fishing Chimes 29(8): 48 -51.

Haniffa M A, Dhanaraj M, Muthu Ramakrishnan C, Arunsingh V and Manikandaraja D.

2010. Fecundity of C. striatus (Bloch 1793) collected from Tambraparni River. Fishing

Chimes 30(7): 7-8.

Haniffa M A, Bilal Ahmad Paray and Manikandaraja D. 2010. Training on Seed production,

feed formulation, and culture of murrels. Fishing Chimes 30(8): 35-38.

Haniffa M A, Bilal Ahmad Paray and Manikandaraja D. 2010. ICAR-NAIP sub-project

workshops on seed production, feed formulation and disease management in murrel culture.

Fishing Chimes 30(8): 39-42.

Haniffa M A. 2012. ICAR- NAIP Sponsored One day Training Programme on – Seed

Production, Feed Formulation and Culture of Murrels: 22 Sept 2011. Fishing Chimes 31(12):

18-20.

Kumar K, Kumar R, Mohanty U L and Sahu A K. 2013. Sal seula macchara janla utpadan

(Odia) (Seed production of murrel) Meenalok FISHCOPFED, Govt. of India, January-March

2013, p 10-13.

10. Any other information : Nil

Signature of PI / CCPI

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73

Proforma -1

Validation of Developed/ Released/ Adopted Production Technologies/ Innovations

(Page limit: 3 pages/technology)

1. Title of the Sub-project: A Value Chain on Murrel Production in Tamilnadu and

Orissa (F.No.1(5)/2007-NAIP dt. 22 August, 2008).

1. Name of CPI/CCPI:

CPI:Dr.M.A.Haniffa, St.Xavier‟s College (Autonomous), Palayamkottai, Tamil Nadu.

CCPI: Dr. K. Altaff, Principal, The New College, Chennai.

2. Title of the Technology: Live Feed Culture and Preparation of Formulated Feed.

3. Information on Existing farming Systems, Practices, Productivity Levels and Income in the

Target Area:

At present most of the fish farmers are using the live feed collected from the natural

water bodies. Since there is seasonal variations in the ponds one can not meet the need of the

fish farmers all the time. Moreover this type of practice is not advisable as it may lead to the

introduction of several pathogenic organisms into the hatchery which will have a very bad

effect on the seed production. Culture of live feed organisms under controlled conditions will

overcome the constraints of the wild caught live feed, which is a common practice among the

fish farmers.

Channa striatus is a carnivorous fish, and is commonly fed on trash fish, which in turn

leads to a high cost of feed production for the culture of this fish. Thus pressure on low value

trash fish supply increases the price and makes the production system unstable and

unsustainable. In practical diets the protein content is often provided by fish meal resulting in

high production costs and this can be addressed by studies on use of cheaper ingredients as

protein sources and use of mixed feeding schedules for better protein utilization. Preparation of

formulated feed using local ingredients will lead to the production of low cost feed and increase

the income of the fish farmers who are practicing the culture of this fish and this may lead to

further extension of the culture practice of this fish by other farmers. At present very little

information is available on nutrition of C. striatus. However information on rearing of C.

striatus exclusively on formulated feed is scarce and inconsistent. Investigations on the

influence of dietary protein level on the culture performance need to be carried out. For most

practical applications, evaluation of production diets can be easily done in feeding trials.

4. Key Intervention(s) Introduced:

Succeeded in Mass culture of zooplankton, Vermiculture and Tufibex.

Culture of cladocerans and copepods (Ceriodaphnia cornuta, Moina micrura and Daphnia

carinata and Thermocyclops decipiens) was succeeded.

Density of zooplankton /litre: Cladocerans (8,000/l), Copepods – (7,000/l), Rotifers

(10,000/l) were produced in fiber tanks of 10,000 l capacity.

5. Results

Status of Dissemination/ Commercialization; and, Extent of Adoption and Success, If

Applicable; with Supporting Data (with Tables and Photographs as Annexure):

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74

The branchipod Moina micrura was mass cultured using three different concentrations

(250 ppm, 500 ppm and 1000 ppm) of chicken manure in the medium for 21 days. The

maximum density of 1694.44± 9.68 was obtained on the 9th

day of the inoculation at the

concentration of 250 ppm and the number of plankton declined gradually afterwards. In 500 ppm

concentration, the maximum density (7296.88 ± 54.35) appeared on the 11th

day while in the

third concentration, maximum density (1510 ± 15.27) was observed on the 14th

day of

inoculation. Young Moina are less than 400µm in size, approximately of the same size of the

adult rotifers.

6. Brief Description of Technology for Release:

The failures with dry diets in freshwater fish larviculture are initially attributed to

deficient enzymatic equipment possessed by the fish larvae during the initial days of life.

Complete suppression of live prey in larval rearing of fish still remains an important challenge.

As the cladocerans are considered to be suitable live feed for fish larvae, for finding a cheap and

suitable medium for culture of Ceriodaphnia cornuta studies were carried out for 21 days using

chicken manure whereupon C. cornuta population ranged between 50±2 and 10,232±202 Ind/l

(Individuals/litre). The culture peaked on the 17th

day producing the maximum density of

10,232± 202 Ind/l suggesting chicken manure as a suitable medium at 700 ppm for mass culture

of cladocerans.

Culture performance of larval snakehead (Channa striatus) was examined by feeding

them with cladocerans (C. cornata, Miona micrura and Daphnia carinata), and Artemia nauplii

as individual and mixed diets for 28 days. Use of cladocerans for early larval rearing of Channa

striatus reduced the expenditures met on seed production, as the nauplii of brine shrimp

Artemia which are extensively used in hatcheries are exotic and highly priced resulting in

higher cost of seed production.

7. Expected Outcome/Impact of the Technology:

(9.1) Expected Increase in Area, Production and Net Income

(9.2) Others

8. Whether findings have been published? If so, give the citation and enclose copy of the

publication.

Altaff K and Mehraj Ud Din W. 2010. Culture of Ceriodaphnia cornuta using chicken

manure as fertilizer: Conversion of a Waste product into highly nutritive protein. Pakistan

Journal of Scientific and Industrial Research 53(2): 89-91.

Mehraj Ud Din W and Altaff K. 2010. Culture of zooplankton for rearing fish larvae.

Pollution Research 29(2): 91-93.

Mehraj Ud Din War and Kareem Altaff. 2011. High density culture of a cladoceran Moina

micrura (Kurz, 1874) by utilizing poultry manure. Pakistan Journal of Scientific and

Industrial Research 54(3): 131-135.

Mehrajuddin War and Kareem Altaff. Utilization of Poultry excreta for high density

production of Daphnia carinata (King 1853). Cost effective and environmental friendly

technique. Pakistan Journal of Scientific and Industrial Research (Accepted).

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75

Book

Sundararaj V. and Altaff K. 2012. Meen Valarpil Uyirunavugal, p 258 Naveena Velanmei,

Chennai, India.

9. Any other information.

Signature of CPI/CCPI

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76

Proforma -1

Developed/ Released/ Adopted Production Technologies/ Innovations

(Page limit: 3 pages/technology)

1. Title of the sub-project: A Value Chain on Murrel Production in Tamilnadu and Orissa

2. Name of CPI/CCPI:

CPI: Dr.M.A.Haniffa, Director, Centre for Aquaculture Research and Extension (CARE)

CCPIs: Dr. K. Altaff, Principal, The New College, Chennai.

3. Title of the Technology: Larviculture Technology

4. Information on Existing farming Systems, Practices, Productivity Levels and Income in the

Target Area:

Rearing of murrels from the postlarvae to the fingerling stage needs extra care and is a

Herculean task. Three days old post larvae were fed on rotifers for 15 days. Apart from

zooplankton, the larvae were also fed on other live feed organisms like blood worms

(Chironomous larvae), tubifex and earthworms. Vitamins are complex organic substances

usually of low molecular weight, which are essential to a wide variety of metabolic processes.

They are required in trace amounts and are essential for normal fish growth, reproduction and

general health. Fish nutritionists have demonstrated that in order to promote growth, any diet

must include an energy source, essential amino acids, fatty acids, vitamins and minerals.

Presence and activity of certain enzymes may be used as a comparative indicator of survival rate

and development. Information on the intestinal enzyme activities is essential to know the

digestive efficiency of the cultured species at the particular stage of development. Herbal

additives in fish feed are used not only against diseases but also as growth promoters, stress

resistance boosters and preventatives of infections in fish. Moreover, herbal treatment is cost

effective and ecofriendly with minimal side effects.

5. Key Intervention(s) Introduced:

Larviculture using vitamins and herbs as growth promoters and immunostimulators

6. Results

Culture performance of larval snakehead (Channa striatus) was examined by feeding

them with cladocerans (Ceriodaphnia cornata, Miona micrura and Daphnia carinata), and

Artemia nauplii as individual and mixed diets for 28 days. Fish fed Artemia nauplii, C. cornata

and mixed cladocerans showed higher weight gain (15.88± 0.11 mg), (9.72± 0.04 mg and 10.0

± 0.06 mg) during the first week, whereas fish fed C. cornata and M. micrura showed better

weight gain (12.88± 0.21 mg and 11.90±0.09 mg) respectively during the second week. Less

cannibalism was observed in fishes fed with mixed cladocerans in the last two weeks. Use of

cladocerans for early larval rearing of C. striatus reduced the expenditures met on seed

production, as the nauplii of brine shrimp Artemia extensively used in hatcheries are exotic and

highly priced resulting in higher cost of seed production. It was noticed that cannibalism was

reduced at different stages by providing prey of suitable size to the growing fish.

Feeding trials were conducted for rearing C. striatus fry using different feeding regimes

for 30 days viz: fish paste, chicken intestine paste, chopped prawn, cladocerans, cladocerans

with chicken paste and chopped prawn with cladocerans. Fishes were fed ad libitum twice a

day. Significantly high survival rate (90%) and better weight gain (354.55± 43.79%) were

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77

observed in the fry fed on chopped prawn with cladocerans followed by those fed on

cladocerans with chicken intestine paste (336.88± 44.25%). High mortality was recorded in fry

fed with fish paste and chicken intestine paste without cladocerans. Hence, the importance of

live feed in the survival and growth of murrel fry is inevitable.

Feeding experiments were conducted on the postlarvae of C. striatus with two different

live feeds copepod (Thermocyclops decipiens) and cladocerans (Ceriodaphnia cornuta and

Moina micrura) individually and in mixture at the rate of 500±50 Ind./L for 30 days. Results

indicated better weight gain (951.85±28.77%) and survival (92%) of postlarvae fed with mixed

live food than individual live feed organisms.

Fry and fingerlings were fed with four types of feed viz., chicken intestine, fish waste,

chicken intestine & fish waste together (50:50) and zooplankton (Thermocyclops decipiens,

Ceriodaphnia cornuta & Moina micrura ) at 5% BW to examine their effects on survival and

growth for 30 days. Better weight gain of 115.32 ±1.35g for chicken intestine feed in case of

fingerlings and 253±1.67 g for live feed in case of fry were observed suggesting the preference

of live feed by fry and chicken intestine by fingerlings over other feeds.

The highest survival rate (93%) was observed in (93.5±2.56%) vitamin C and

(93.7±1.81%) Vitamin - Mineral mix incorporated diets as illustrated in Table 9

After 45 days, there was a significant difference between the mean weights as a

function of different experimental diets (D1 – D5), when compared to control diet. The highest

weight gain (11.46±0.52g) was noticed in Channa striatus fed on diet incorporated with

vitamin C. Among the experimental fishes, the highest protein content was noticed in vitamin C

incorporated diet (36.91%), followed by other diets. The highest carbohydrate content was

found in control diet (17.57%) whereas the lowest carbohydrate content was found in vitamin C

(13.14%). Fat was found as the highest in control (13.65%) and the lowest in vitamin C

incorporated diet (10.75%). The protease, amylase and lipase activities were significantly

higher in Vitamin C diet as 110.58±2.49 Umg-1

, 53.50±2.49 Umg-1

and 93.80±1.42 Umg-1

respeectively.

Growth

parameters

Control

(D1)

Folic acid

Diet (D2)

Vitamin B

Diet (D3)

Vitamin C

Diet (D4)

Vitamin -

Mineral mixed

Diet (D5)

Initial

Weight (g)

10.8±0.09 10.6±0.12 10.7±0.45 10.6±0.67 10.8±0.36

Final

Weight (g)

17.83±0.11 19.11±0.34 20.15±0.49 22.06±0.52 20.93±0.48

Survival

rate (%)

82.3±2.36 92.1±1.56 91.6±1.81 93.5±2.56 93.7±1.81

Weight

gain (g)

7.03±0.11 8.61±0.31 9.45±0.45 11.46±0.52 9.13±0.39

SGR 1.11±0.28 1.33±0.09 1.56±0.14 1.62±0.23 1.49±0.04

FCR 3.42±0.01 2.75±0.02 2.51±0.04 2.06±0.01 2.43±0.03

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78

Effect of dietary herbal additive Phyllanthus niruri, resulted better growth performance

and immunostimulation in Channa striatus fingerlings. All growth and hematological parameters

increased in response to supplementary diet Phyllanthus niruri. In P. niruri fed group, the mean

weight gain (5.166 g) and specific growth rate (3.63) were the highest when compared to the

control group (2.915). Incorporation of P. niruri diet increased haemoglobin ( Hb) (26.85%), red

blood cell count (43 ±0.38), white blood cell count (29 ±0.25) and mean corpuscular

haemoglobin concentration (33.23g/dl). Haematocrit (36.41 ±4.2), mean corpuscular

haemoglobin (2.81) and mean corpuscular volume (8.46fl) also increased. P. niruri acted as a

potent immunostimulant, by inducing the blood parameters in C. striatus.

In the next study, diets were prepared using the feed additives viz: bacterium Bacillus

subtilis, Indian almond Terminalia catappa and aloe Aloe vera. C. striatus fingerlings fed on

control diet showed 2.881g weight gain, whereas those supplied with T. catappa diet gained

6.319g body weight followed by A. vera diet (2.915g) and B. subtilis diet (2.894g). The specific

growth rate (3.96) and condition factor (0.8) of T. catappa fed groups were higher than that of

other supplemented fed groups. During post A. hydrophila challenge, highest survival rate was

observed in Aloe fed group (100%) followed by B. subtilis (90%), T. catappa (90%) and control

(60%) fed groups. Survival of striped murrel fed diets supplemented with aloe was the greatest

post challenge with A. hydrophila. Survival of striped murrel fed diets supplemented with B.

subtilis and Indian almond was greater than that of fish fed on control diet. The improved

survival suggests that production of metabolites by probiotics suppressed the pathogenicity of A.

hydrophila and ultimately resulted in better survival and growth of the host.

The cellulolytic, amylolytic and proteolytic activities of bacteria were isolated and

enumerated from the gastrointestinal tract of murrel (C. striatus) fingerlings (22±0.43g) fed with

herbals like Murraya Koenjii, Sesbania grandiflora and Amaranthus thandu (I – III), probiotics

like B. coagulans, B. subtilis and mixed probiotics (IV – VI) and chicken intestine diet (VII)

with group VIII as control. At the end of culture, the maximum average growth of group – IV

fish was higher (3.51±0.20) than that of the control – VIII (2.01±0.20). At the end of

experimental period, the survival in group VII was 98% whereas in control, it was 90%. The

cellulolytic acitivity was higher in group-IV fishes (93.2±0.81Umg-1

), than control group

(5.9±0.84Umg-1). The amylolytic and the proteolytic activities were higher i.e., 3.3±0.53Umg

-1

and 53.4±0.31 Umg-1

in group III respectively and the differences were statistically significantly

(p<0.05). The results of the present study showed that the chicken intestine and B. subtilis have

better impact on murrel culture

7. Brief Description of Technology for Release:

Rearing of murrels from the post larvae to the fingerling stage needs extra care and is a

Herculean task. Three days old post larvae were fed on rotifers for 15 days. Apart from

zooplankton, the larvae were also fed on other live feed organisms like blood worms

(Chironomous larvae), tubifex and earthworms. Boiled chicken intestine paste and fish paste

were supplied as feed for the larvae from the 16th

day onwards instead of live feed. From 30th

day

onwards, the fry were fed with cooked and minced chicken intestine and semi-moist pellets and

were later trained on pelleted feed (semimoist) during fingerling stage. There is no readymade

feed for murrels in market, since they are carnivorous, piscivorous and cannibalistic. Formulated

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79

diet containing fish meal (anchovy and jawala) – 60%, wheat flour – 10%, rice flour - 10% and

soya flour 20% respectively with 43% of protein was prepared for murrels. Feed pellets

prepared using pelletizer ranged between 2.5 -5.0 mm were readily accepted by murrel

fingerlings.

Feeding experiments conducted on the postlarvae of C. striatus indicated better weight

gain and survival while fed with mixed live food (a copepod (Thermocyclops decipiens) and

cladocerans (Ceriodaphnia cornuta and Moina micrura) than individual live feed organisms.

Moreover, it had been observed that fry preferred live feed whereas fingerlings preferred chicken

intestine over other feeds.

Vitamin supplemented diets resulted in an increase in weight gain and specific growth

rate of C. striatus. The results showed that survival and growth rate of fingerlings increased

(p < 0.05) with 200 mg of vitamin C / kg feed. The addition of vitamin-C in diet improved

digestive enzymes, including protease, amylase and lipase, which have consequently resulted in

better growth performances. Different vitamins could affect the enzyme activities differently,

and Vitamin C induced a stronger stimulation than the others. This stimulation of amylase and

protease activity was correlated with the growth performance.

Antibiotics play an important role in the disease management of aquaculture practices;

however antibiotic resistance develops readily in pathogens following antibiotic treatment.

Medicinal herbs play an alternative role to tackle this problem. To enhance the survival and

growth and to improve the health of the fingerlings, the pellets have been incorporated with

herbs (Phyllanthus niruri, Aloe vera, Terminalia catappa, Aloe vera etc) and probiotics (Bacillus

subtilis, Saccharomyces cervisiae, Lactobacillus helveticus, L. acidophilus, L. plantarum, L.

sporogens, Candida utilis etc). Probiotic and herbal incorporated feeds showed good results as

means of higher survival rate, growth and immunity of C. striatus fingerlings suggesting their

importance in larval rearing.

8. Expected Outcome/Impact of the Technology:

Live feed organisms are suitable for fry upto 20 days and hence large scale culture of

zooplankton will meet the growing demand. Moreover, in the case of pelleted feed,

incorporation of feed additives like vitamins, herbs and probiotics help in the enhancement of

survival and immunity of the fingerlings. This in turn will improve the health status if

fingerlings and the economic conditions of the farmers.

9. Whether findings have been published? If so, give the citation and enclose copy of the

publication.

Research Articles:

Mehraj Ud Din W, Altaff K and Haniffa M A. 2009. Study on the growth and survival of

Channa striatus post larvae (Bloch) using live feed. Bangladesh Journal of Fisheries Science

13(2): 131-135.

Mehraj Ud Din W, Altaff K and Haniffa M A. 2009. Comparative study on growth of Channa

striatus fry and fingerlings using different feeding regimes. Journal of Aquatic Biology

24(2): 173-176.

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80

Mehraj Ud Din W, Altaff K and Haniffa M A. 2010. Evaluation of growth and survival of fry

of Channa striatus (Bloch) using different feeding regimes and cladoceran live feed. Journal

of Experimental Zoology India 13(1): 225-227.

Mehraj Ud Din War, Kareem Altaff and Mohammed Abdul Khader Haniffa. 2011. Growth

and survival of larval snakehead Channa striatus (Bloch, 1793) fed different live feed

organisms. Turkish Journal of Fisheries and Aquatic Sciences 11(3):523-528.

Muthukrishnan Dhanaraj and Mohamed Abdul Kather Haniffa 2011. Effect of probiotics on

growth and microbiological changes in snakehead Channa striatus challenged by Aeromonas

hydrophila. African Journal of Microbiology Research 26(5):4601-4606.

Haniffa M A, Kavitha Bharathi B, Viji Margaret I, Bilal Ahmad Paray and Manikandaraja D.

2013. Effect of probiotics and certain herbal additives on growth, survival and disease

resistance of Channa striatus challenged by Aeromonas hydrophila. World Aquaculture

44(2): 64-67.

Ramesh S, Chelladurai G and Haniffa M A. 2013. Isolation of enzyme producing bacterial

from gut of Channa striatus fed on different herbs and probiotics diet. International Journal

Pharmacy and Pharmaceutical Sciences 5(4): 195-198.

Mehrajuddin War and Kareem Altaff. 2013. Preliminary Studies on the Effect of Prey Length

on Growth, Survival and Cannibalism of Larval Snakehead, Channa striatus (Bloch, 1793).

Pakistan Journal of Zoology 45(2): 1-6.

Haniffa M A, Viji Margaret I, Kavitha Bharathi B and Jeya Sheela P. 2013 Dietary

administration of herbal additive Phyllanthus niruri on growth performance and

haematological changes in striped snakehead Channa striatus. World Aquaculture

(Accepted)

Haniffa M A, Jeya Sheela P, Vigneswaran R and Kavitha K. 2012. Effect of Vitamins on

Digestive Enzyme Activities and Growth Performances of Striped Murrel Channa striatus.

Iranian Journal of Fisheries and Aquatic Sciences

M.Sc. Dissertations:

Mohamed Ansari A. 2009. „Effect of water probiotics on intestinal microflora and growth,

Aeromonas hydrophila challenged Channa striatus exposed to different salinity.‟ M Sc

thesis, Jamal Mohamed College (Autonomous), Thiruchirapalli, Tamil Nadu, p 55.

Suganya K R. 2010. „Digestive enzyme activities of Channa striatus fingerlings fed with

selected medicinal herbs.‟ M Sc thesis, Nandha Arts and Science College, Erode, Tamil

Nadu, p 29.

Tamil Elakkiya B. 2010. „Digestive enzyme activities of Channa marulius fingerlings fed

with selected probiotics.‟ M Sc thesis, Nandha Arts and Science College, Erode, Tamil Nadu,

p 32.

Vigneshwaran R. 2011. „Effect of dietary vitamins on growth and protein variability of

Channa striatus fingerlings.‟ M Sc thesis, Jamal Mohamed College (Autonomous),

Thiruchirapalli, Tamil Nadu, p 62.

PhD Theses:

Dhanaraj M. 2009. „Probiotics as immunostimulants in striped murrel Channa striatus.‟ Ph

D thesis, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, p 127.

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Mehraj Ud Din War. 2012. „Aspects of development and larval rearing of snakehead murrel

Channa striatus (Bloch 1793) using different feed.‟ Ph D thesis, Madras University, Chennai,

Tamil Nadu, p 108.

Bilal Ahmad Paray. 2012. „Histological studies on ontogenic development and larval rearing

of striped murrel Channa striatus.’ Ph D thesis, Manonmaniam Sundaranar University,

Tirunelveli, Tamil Nadu, p 165.

Popular Articles:

Haniffa M A, Bilal Ahmad Paray and Manikandaraja D. 2010. ICAR-NAIP sub-project

workshops on seed production, feed formulation and disease management in murrel culture.

Fishing Chimes 30(8): 39-42

Haniffa M A. 2012. ICAR- NAIP Sponsored One day Training Programme on – Seed

Production, Feed Formulation and Culture of Murrels: 22 Sept 2011. Fishing Chimes 31(12):

18-20

10. Any other information.

Nil

Signature of CPI/CCPI

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82

Proforma -1

Validation of Developed/ Released/ Adopted Production Technologies/ Innovations

(Page limit: 3 pages/technology)

1. Title of the Sub-project: A Value Chain on Murrel Production in Tamil Nadu and Orissa

(F.No.1(5)/2007-NAIP dt. 22 August, 2008).

2. Name of CPI/CCPI:

CPI:Dr.M.A.Haniffa, St.Xavier‟s College (Autonomous), Palayamkottai, Tamil Nadu.

CCPIs:Dr.A.K.Sahu, Dr.Kuldeep Kumar & Dr.Rajesh Kumar,CIFA, Bhubaneswar.

3. Title of the Technology: Murrel Growout Culture Technology

4. Information on Existing farming Systems, Practices, Productivity Levels and Income in the

Target Area:

In Indian major carps are easily avalible for consumption; hence, the fish farmers are

interested mainly in carp culture. According to available information and our baseline survey, it

was evident that there was no scientific murrel culture practices followed in Tamil Nadu. Usually

murrels are captured during summer (when ponds and lakes dry) and brought to local markets.

There is a traditional practice to catch murrels from rice fields or derelict water bodies in small

quantities for consumption. Since, most of the murrel production comes from the wild, it is

difficult to estimate the exact contribution to the individual farmers‟ income.

5. Key Intervention(s) Introduced:

Earthern ponds ranging from 0.1 ha to 0.4 ha with a depth of 1m are recommended for

murrel culture.

Murrel farmers supply semimoist feed as dough in trays and gunny bags

52 farmers practiced murrel culture in Tamil Nadu and they harvested table size murrels in a

total area of 266.56 ha with a production of 27.67 tons in Tamil Nadu.

1119 farmers were trained for murrel culture through twenty one training programmes and

twelve workshops in Tamil Nadu and Orissa

The CIFA has developed it‟s grow out culture technology and it has been disseminated to the

farmers of Orissa under the NAIP project.

6. Results:

Status of Dissemination/ Commercialization; and, Extent of Adoption and Success, If

Applicable; with Supporting Data (with Tables and Photographs as Annexure):

Earthen ponds ranging from 0.1 ha to 0.4 ha with a depth of 1m with a stocking density

of 1/m2

were suggested suitable for murrel culture. Since murrels are air breathing, they survive

in oxygen depleted water bodies. For commercial murrel culture, earthen ponds of various

dimensions (depending on land availability) are recommended. Once the fingerlings were trained

for feeding on semi- moist or pelleted feed (consisting of fish meal, soy flour, wheat flour, rice

flour and tapioca), they were introduced into the culture ponds.

At present murrel farmers supply the feed as dough in trays & gunny bags. ICAR-NAIP

Research team has trained murrel fingerlings to take pelleted feed at CARE Aqua farm &

stakeholder‟s farms. Usually the culture period is about 10 months to obtain table size fish of

750g weight. It is better to harvest before one year to avoid excess cost of feed. Under the

subproject, 52 farmers practiced murrel culture in Tamil Nadu and they harvested murrels in a

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83

total area of 266.56 ha with a production of 27.67 tons in Tamil Nadu. The market price of table

size murrel varied from Rs.150/- from the inception of the project to Rs.700/- by 2014 depending

upon the area, water availability and demand for murrels.

Table 4 Yearwise Table size Murrel Production in Tamil Nadu

Year No. of

farmers

harvested

Total

Area

(ha)

Harvested

Murrels

(tons)

Income

Generated

(Rs.)

Production

in tons/ ha/

yr

Income

generated

per ha/ yr

2010-2011 19 250 17.34 33,51,450/- 0.069 13,405/-

2011-2012 20 7.74 5.73 16,13,000 0.740 2,08,398/-

2012-2013 13 7.92 4.6 13,26,200 0.580 1,67,450/-

7. Brief Description of Technology for Release:

Murrel Grow out culture technology:

Usually the culture period is about 10 months, to obtain table size fish of 750g weight. It is

better to harvest before one year to avoid excess cost of feed. Under the subproject, 52 farmers

are practicing murrel culture in Tamilnadu.

Table 10 Economics of Large Scale Murrel (C. striatus) Table Size Fish Production – CIFA (2010)

S.No Variable Unit cost (Rs.) Actual

Value (Rs)

Culture Characteristics

1 Pond (Lease for 3 Years) For 3 Years- Rs 10,000

2 Pond Area 1 Acre 3300

3 Pond preparation (dyke repair, liming,

manuring etc.)

10,000

3 Stocking size 40g (Rs. 5.00 per fingerling) -

4 Stocking density (Murrel fish) 4000 fingerlings/acre 20,000

5 Feed (Poultry offal & Rice bran- 8:2) For a period of 9 months 35,000

6 Labour (feeding & other works) Weekly 4 man days (@ Rs

170)- 160X170

27,200

7 Post stocking management (medicine,

fertilization etc)

- 8000

8 Cost of Harvesting - 3000

9 Total expenditure 1,06,500

Murrel fish Production:

10 Production of table size (@ 60%

survivability with average weight of

0.80 Kg)

1,920 Kg

11 Marketing of Harvested murrel @ 120/Kg (1,920X120) 2,30,400

Total earning 2,30,400

Total Expenditure 1,06,500

Net Profit 1,23,900

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84

8. Expected Outcome/Impact of the Technology:

(8.1) Expected Increase in Area, Production and Net Income

Table 11 Economics of Small Scale Production of Table Size Murrels – CARE (2013):

Item Rupees

Expenses

A. Capital cost:

1, Cement Pond construction

25‟x 10 x 3‟= 875CFT. Rs 55/CFT : 48,125

2, Motor pipelines : 15,000

3, Fencing & net : 3400

---------------

Total Expenses : 66,525

---------------

B. Operational cost:

1, Cost of fingerlings 500 x Rs 4/individual : 2000

2, Feed cost (300 days X Rs 25/day) : 7500

3, Electricity : 1000

4, Maintenance cost : 3200

--------------

Total Expenses : 13,700

---------------

Total Expenses of Aand B : 80225

Income

Fish sales of 100kg at the rate of Rs500/Kg : 50,000

Total Expenses other than capital cost : 13700

---------------

Net Profit Per year : 36,300

---------------

The harvested murrels 100 Kg were sold at a rate of Rs. 500/Kg and the net profit was

Rs. 36,000/- for an investment of Rs. 80,000. As per availability of area in the backyard, one can

either increase the area of the culture tank (250 m2

– 100 m2) or the number of culture tanks (1 -

4). For instance if 100 m2

area is available, at the backyard, farmer can earn about Rs

150,000/year by selling 400 murrels.

In Orissa, Women self help group adopted murrel culture technology and harvested good

production. They consumed part of the produce and remaining was sold. This helped them to

improve their family nutritional security as well as livelihood. This acted as catalyst and they

took other community ponds, which was lying idle since many years, on lease for murrel culture.

A few SHGs has earned good profit from murrel culture and now many more farmers are coming

forward to adopt this technology. This technology is giving more net profit in comparison to carp

culture.

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85

Table 12 Details of murrel production in Orissa

(8.2) Others Nil

7. Whether findings have been published? If so, give the citation and enclose copy of the

publication.

Research Articles

Kuldeep K, Lalrinsanga P L, Sahoo M, Mohanty U L, Kumar R and Sahu A K. 2013. Length-

Weight Relationship and Condition Factor of Anabas testudineus and Channa Species under

Different Culture Systems. World Journal of Fisheries and Marine Science 5(1): 74-78.

Books

Sundararaj V and Haniffa M A. 2010. Veral Valarppu, p 248 Naveena Velaanmai, Chennai,

India.

Haniffa M A. 2011. Aquatic Resources and Aquaculture, p 328 Dominant Publishers and

Distributors Pvt. Ltd., New Delhi, India

Farmer/SHG/NGO Gross Production Production

(Kg/yr./ha.)

Women Self Help Group (comprising of

44 members) named Narayani Nari Shakti

Mahila Mandal Dist. – Khurda

1,213 Kg 1,895

Unnayan (NGO), Puri. 47 Kg 2,350

Maa Mangala Yuba Atmasahayak Gosthi

(comprising of 11 members) Village-

Tarapi, P.O.-Soran, Via- Kuhuri, Block-

Chilka, Dist.- Khurda

2,000 Kg 2,583

Mr. Amit Mohanty, Puri. 105 Kg 1,050

Mr. Brundaban Swain, Barkul, Dist.-

Khurda

546.8 Kg 1,367

Mr Sisir Kant Biswal, Konark 600Kg 1,500

Mrs. Pratima Barala (SHG; comprising of

10 members)

296.6 Kg 1,483

Mrs. Satyabhama Patnayak (SHG;

comprising of 10 members)

162.8Kg 1,080

Mr Prafulla Subudhi, Tarapi, Khurda 250kg expected 2,100

Mr Brajabihari sahoo, Daspalla, Nayagarh 200 kg expected 1,800

Mr Trinath Sahoo, Daspalla 150 kg expected 1,500

Mr Jalandhar Ghanta, Daspalla 150 kg expected 1,500

CIFA farm, Kausalyaganga, Bhubaneswar 400 Kg harvested &

700 Kg expected

2,562

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86

K. Kumar, R. Kumar, S. Saurabh, M. Sahoo, A. K. Mohanty, P. Lalrinsanga, U. L. Mohanty,

A. K. Sahu and P. Jayasankar. 2012. Snakehead: Fishes fact sheets. p 38 Central Institute of

Freshwater Aquaculture (ICAR), Kausalyaganga, Bhubaneswar.

Book Chapters

Eknath A E, Kumar K, Jena J K, Mohanty U L, Kumar R, Jahan N and Sahoo M. 2010.

Murrel: An ideal fish for mass production (in) souvenir of Assam Matsya Mahotsav,

organized by the Department of Fisheries, Govt. of Assam during 30th

Jan- 1st Feb 2010. p 5-

8

Kumar K, Sahu A K, Kumar R, Mohanty U L, Sahoo M and Jahan, N. 2011. Murrel Culture

in Odisha-A Humble Beginning (in) Aquaculture Innovators, p 105-112, Radheshyam et al.

(Eds), Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, India.

Kumar R, Kumar K, Mohanty U L and Sahu A K. 2012. Murrel (Channa striatus): Potential

candidate species for nutritional security and livelihood generation. (in) Souvenir: The world

food day, p 49-51, S J Swain et al. (Eds), Odisha Krishak Samaj, Bhubaneswar, Odisha.

Kumar K, Kumar R, Mohanty U L, Saurabh S, Sahu B B, Sahoo M, Mohanty A, Sahu A K

and Jayasankar P. 2013. Snakehead fishes: Alternative candidate fish for diversification

aquaculture. pp- 119-132 (in) Aquaculture: New Possibilities and Concerns, P-187 Sinha,

V.R.P., Jayasankar, P. (Eds), Narendra Publishing House, Delhi, India.

Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) Ka beej utpadan ebm Palan

(Hindi) (Seed Production and culture of murrel) (in) Training manual on Jalkrishi mein

Dakhata Vikash, p 50-54. Published by Director, CIFA, Kausalyaganga, Bhubaneswar, India

on 15-19 July 2013.

Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) Ka beej utpadan ebm Palan

(Hindi) (Seed Production and culture of murrel) (in) Training manual on Unnat mithajal

matya palan, p 54-57. Published by Director, CIFA, Kausalyaganga, Bhubaneswar, India.

12-16 December 2013.

Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) ka beej utpadan ebm Palan

(Hindi) (Seed Production and culture of murrel) (in) Training manual on ‘Mithajal Matya

palan mein unnat praudhogiki’, p56-59. Published by Director, CIFA, Kausalyaganga,

Bhubaneswar, India. 20-24 December 2013.

Popular Articles:

Haniffa M A, Dhanaraj M, Muthu Ramakrishnan C, James Milton M, Arunsingh S V and

Ajaz Ali. 2009. Backyard Murrel Culture for Women. Infofish International 6:14 -16.

Haniffa M A, Seenappa D, Manikandaraja D and James Milton M. 2011. Murrel Farming

training to Fish farmers of Karnataka. Fishing Chimes 30(10&11): 52-53.

Kumar K, Eknath A E, Sahu A K, Mohanty U L, Kumar R, Sahoo M and Jahan N. 2011.

Snake heads: Challenging fish for diversification of fish farming. Fishing Chimes 31(1): 110-

113.

Haniffa M A. 2011. Popularization of Murrel Farming among Fish Farmers of Hyderabad.

Fishing Chimes 31(2): 27-29

Haniffa M A. 2011. A value chain on murrel production for income generation. Fishing

Chimes 31(5): 19-21.

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87

Haniffa M A. 2012. ICAR- NAIP Sponsored One day Training Programme on – Seed

Production, Feed Formulation and Culture of Murrels: 22 Sept 2011. Fishing Chimes 31(12):

18-20.

Murrel Culture in Odisha: A Humble Beginning. Kuldeep Kumar, A. K. Sahu, Rajesh

Kumar, U. L. Mohanty, M. Sahoo & Noor J. (2011): Aquaculture Innovators. Central

Institute of Freshwater Aquaculture, ICAR, Kausalyaganga, Bhubaneswar. pp. 106-113.

Haniffa M A. 2010. Viru Viru Labam Tharum Viral Valarppu (Tamil) (Murrel Fish Culture

for Fast Income). Pasumai vikadan, 10.11.2010, p 4-9

Haniffa M A. 2011. Villangam Illatha Viral Valarppu (Tamil) (Harmless Murrel Fish

Culture). Pasumai vikadan, 25.09.2011, p 40-41

Haniffa M A. 2011. Viral Meen Valarppu Payirchi Mugaam (Tamil) (Murrel Fish Training).

Valarum Thamilagam, November 2011, p 34-35.

Haniffa M A. 2011. Pambuthalai Viralmeen, Meen Valarporukku Oru Varaprasatham

(Tamil) (Snakehead murrel fish culture- a boon to fish farmers). Valarum Thamilagam,

December 2011, p 36-37

Haniffa M A. 2011. Veeru Nadai Podum Viral Valarpu (Tamil) (Fast Growing Murrel

Culture). Pasumai vikadan, 10.12.2011, p 28-31.

Kumar K, Kumar R, Mohanty U L and Sahu A K. 2012. Murrel Maccha Chasa O tara

Prasara (Odia) (Murrel farming and its popularization). Meenalok, FISHCOPFED, Govt. of

India, October-December 2012, p3-6.

Haniffa M A. 2012. Thennaiku Idail Viral (Tamil) (Murrel Culture amidst Coconut Trees).

Pasumai vikadan, 10.02.2012, p 29-31

8. Any other information : Nil

Signature of PI / CCPI

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88

Proforma -1

Validation of Developed/ Released/ Adopted Production Technologies/ Innovations

(Page limit: 3 pages/technology)

1. Title of the Sub-project: A Value Chain on Murrel Production in Tamilnadu and Orissa

(F.No.1(5)/2007-NAIP dt. 22 August, 2008).

2. Name of CPI/CCPI:

CPI:Dr.M.A.Haniffa, St.Xavier‟s College (Autonomous), Palayamkottai – 627 002.

CCPIs:Dr.A.K.Sahu, Dr.Kuldeep Kumar & Dr.Rajesh Kumar, CIFA,Bhubaneswar.

3. Title of the Technology: Murrel Disease Management Technology

4. Information on Existing farming Systems, Practices, Productivity Levels and Income in the

Target Area:

Murrels are often affected by the dreadful disease Epizootic Ulcerative Syndrome (EUS)

and encounter heavy loss to capture and culture fisheries. The primary causative agent of EUS is

the fungus Aphanomyces invadans and the opportunistic bacterial pathogen is Aeromonas

hydrophila characterized as virulent or cytotoxic invades the blood stream and causes lethal

septicemia resulting heavy mortality. Moreover, Vibrio sp., Pseudomonas sp. and Salmonella sp.

and virus have also been detected in EUS affected murrels. The development of drug resistance

as well as appearance of undesirable side effects of certain antibiotics in EUS affected murrels

have led to the search of new antibacterial agents, particularly medicinal plants as potential

source of new anti-microbial agents, since they are safe without side-effects and also ecofriendly.

5. Key Intervention(s) introduced:

Herbal pastes were prepared using medicinal herbs like Coleus aromaticus, Wrightia

tinctoria, Azadiracta indica, Ocimum sanctum, Curcuma longa and A. marmelos for treating

EUS of murrels.

Fishes with severe mortality recovered completely within 7 days of the herb treatment

6. Results

Status of dissemination/commercialization; and, extent of adoption and success, if applicable;

with supporting data (with tables and photographs as annexure):

The crude extracts of a medicinal herb viz; Wrightia tinctoria was successfully used to

prevent EUS occurrence and enhance survive during larviculture and growout culture. Leaves of

W. tinctoria applied as paste on the lesion and ulcers externally cured EUS within four to six

days. The antibacterial and antifungal activity of W. tinctoria was studied against pathogens

Pseudomonas fluorescens, Aeromonas hydrophila, Staphylococcus epidermidis, Aeromonas

salmonicida, Aphanomyces invadans and Vibrio alginolyticus. Phytochemical screening of the

leaves revealed the presence of steroids, reducing sugars, alkaloids, phenolic compounds,

flavonoids, saponins and tannins. The TLC chromatogram of the extracts revealed several

coloured bands. The methanol extracts showed efficient antibacterial activity15.6 ±0.3/67.1

(Inhibition zone diameter in mm/RIZD in %) against A. hydrophila. The minimum Inhibitory

concentration of the extract against the pathogens was determined. Growth of A. invadans was

inhibited by 85-90%. The herbal paste applied topically on the lesions showed a positive effect

by controlling and curing the lesions within five days. Following 2nd and 3rd

day post application

of herbal paste, healing symptoms were noticed and signs of dermal lesions and ulcers vanished

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89

and the wound was completely healed by the 5th

day. No mortality was observed during the

course of the study. It is therefore suggested that the active extract of W. tinctoria could be taken

to the next step of bioassay guided purification to characterize the novel antimicrobial agents.

In another consecutive study, the medicinal plant Coleus aromaticus was used in treating

EUS infected giant murrels C. marulius showing symptoms like lesions and deformed fins at the

dorsal side with reddish spots on the ventral side. The herbal paste was applied topically on the

wound surfaces of diseased murrels for 10 days. From 5th

day onwards, the treated murrels

showed good signs of healing like disappearance of lesions and reddish spots and recovery of

affected fins, thus recommending the topical application of herbs for effective treatment of

diseased murrels.

A preliminary study was conducted during winter months to know the effects of plant

crude extracts on growth, survival and health status of Channa striatus fingerlings in cemented

cisterns of capacity 1000 L in field condition. The herbal crude extract was prepared separately

from the leaves of Ocimum sanctum (Tulsi), Aegel marmelos (stone apple) and rhizome of

Curcuma longa (turmeric) and kept in sealed poly packs under refrigeration. C. striatus

fingerlings (6.15 + 0.2g) were fed on murrel feed containing different medicinal plant extracts

for 60 days. The control group was fed same feed but without herbal extract. Fishes were

observed fortnightly for growth, survivability, gross health examination and bacterial total plate

count of whole content of intestine and gills.

Fishes were fed feed containing turmeric showed significantly higher (p<0.05) survival

(93.33 %) in comparison to control (83.33%) and other herbal crude extract fed groups. There

was no significant difference in growth between control group and crude extract fed groups.

Gross morphological examination showed that fishes fed herbal crude extracts were healthy

(shiny body, intact fins, active movement, etc) in comparison to the control group. The microbial

studies revealed that O. sanctum fed fishes contains significantly less (p<0.05) number of

bacteria in gut i.e., total plate count (2.35 x 107

cfu) in comparison to fishes of other herbal crude

extract fed or control (3.40 x 109) groups. Similar result was observed for gills. The study

suggests that the herbal crude extracts could be used for prophylaxis to keep fishes healthy and

also to enhance their survival during fingerling stages.

Table 5 Effect of herbal paste on growth and survival of C. striatus fingerlings.

Treatment Initial mean

weight (g)

Final mean

weight (g)

Growth (%) Survival (%)

C 6.15 + 0.2 31.78 + 0.40 416.7 + 06.44 83.33a + 0.00

T1 6.15 + 0.2 31.17 + 0.86 406.8 + 14.02 86.66ab

+ 4.70

T2 6.15 + 0.2 30.92 + 1.25 390.2 + 39.08 93.33c + 0.00

T3 6.15 + 0.2 30.15 + 2.40 402.8 + 20.35 90.00bbc

+ 4.71

The incidence of Ichthyophthirius multifilis was reported by CIFA, Odisha during the

nursery rearing of striped snakehead. This was successfully controlled with continuous

application of 10 ppt formalin for 5 days. This helped in total eradication of parasites from

infected seed and finally mortality was checked completely.

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90

Acinetobacter baumannii acts as a severe human pathogen. There are only few studies to

date that report it as a pathogen for fish. In the present study, one virulent bacterial strain was

isolated from diseased Channa striatus, from a farm at the CIFA which showed symptoms like

cloudy eyes, pop eye (exophthalmia), opaque lenses and mild ulceration on the whole body

irrespective of sex and size of the fish. Based on morphology, different biochemical tests and

sequence analysis of 16S rDNA segment as well as phylogenetic study, the causative bacterium

(called chE) was identified as A. baumannii. The pathogenicity was further confirmed by

artificial infectivity study (LD50 dose of 10 8.37

CFU/fish). In the drug sensitivity study, this

isolate was highly resistant to many antibiotics. The isolate was also highly resistant to all three

tested heavy metals (Cu+2

, Cr+6

, Hg+2

), thus proving its virulent nature. The involvement of A.

baumannii in disease of Channa species poses a potential threat to freshwater fish farming as an

important emerging pathogen. Further, the resistance to many antibiotics and heavy metals

shown by this isolate poses serious concern.

7. Brief description of technology for release:

The primary causative agent of EUS is the fungus Aphanomyces invadans and the

opportunistic bacterial pathogen is Aeromonas hydrophila characterized as virulent or cytotoxic

which invades the blood stream and causes lethal septicemia resulting heavy mortality. In search

of eco friendly treatment, medicinal plants acts as a potential source of new anti-microbial

agents.

8. Expected Outcome/Impact of the technology:

(8.1) Expected increase in area, production and net income

The technology has been transferred to fish farmers through regular training and workshop

(8.2) Others

8. Whether findings have been published? If so, give the citation and enclose copy of the

publication.

Research Articles

1.1. Muthukrishnan Dhanaraj and Mohamed Abdul Kather Haniffa 2011. Effect of probiotics

on growth and microbiological changes in snakehead Channa striatus challenged by

Aeromonas hydrophila. African Journal of Microbiology Research 26(5):4601-4606.

1.2. Rauta P R, Kumar K and Sahoo P K. 2011. Emerging new multi-drug resistant bacterial

pathogen Acinetobacter baumanii from snake head Channa striatus from India. Current

Science 101(4): 548-553.

1.3. Haniffa M A and Kavitha K. 2012. Antibacterial activity of medicinal herbs against the

fish pathogen Aeromonas hydrophila. Journal of Agricultural Technology 8(1): 205-211.

1.4. Haniffa M Aand Shanthi P. 2012. Phytochemical analysis and antibacterial screening of

medicinal plants against Aeromonas hydrophila. Asian Journal of Pharmaceutical and

Clinical Research

1.5. Haniffa M A, Jeya Sheela P, James Milton M and John De Britto A. 2013. Invitro and

Invivo Antimicrobial Effects of Wrightia tinctoria (Roxb.) R. Br. against Epizootic

Ulcerative Syndrome in Channa striatus. International Journal of Pharmacy and

Pharmaceutical sciences 5(3): 219-222.

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1.6. Haniffa M A, Kavitha Bharathi B, Viji Margaret I, Bilal Ahmad Paray and Manikandaraja

D. 2013. Effect of probiotics and certain herbal additives on growth, survival and disease

resistance of Channa striatus challenged by Aeromonas hydrophila. World Aquaculture

44(2): 64-67.

1.7. Haniffa M A, Viji Margaret I, Kavitha Bharathi B and Jeya Sheela P. 2013 Dietary

administration of herbal additive Phyllanthus niruri on growth performance and

haematological changes in striped snakehead Channa striatus. World Aquaculture

(Accepted).

M.Sc. Dissertations:

Mohamed Ansari A. 2009. „Effect of water probiotics on intestinal microflora and growth,

Aeromonas hydrophila challenged Channa striatus exposed to different salinity.‟ M Sc

thesis, Jamal Mohamed College (Autonomous), Thiruchirapalli, Tamil Nadu, p 55.

Jeyakumar R. 2010. „Studies on the effect of herbal concoction in the therapy of Channa

striatus (Bloch).‟ M Sc thesis, St. Xavier‟s College (Autonomous), Tirunelveli, Tamil Nadu,

p 59.

Ashfaq Ahmed S. 2011. „Studies on pathogenicity and treatment of Channa marulius

infected with Aeromonas hydrophila.‟ M Sc thesis, Jamal Mohamed College (Autonomous),

Thiruchirapalli, Tamil Nadu, p 44.

Imthathulla S. 2012. „A comparative study of Antioxidant enzymes and lipid peroxidation in

selected murrel species challenged with Aeromonas hydrophila.‟ M Sc thesis, Jamal

Mohamed College (Autonomous), Thiruchirapalli, Tamil Nadu, p 26.

PhD Theses:

1.8.Dhanaraj M. 2009. „Probiotics as immunostimulants in striped murrel Channa striatus.‟ Ph

D thesis, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, p 127.

Popular Articles:

5.1 Haniffa M A, Bilal Ahmad Paray and Manikandaraja D. 2010. ICAR-NAIP sub-project

workshops on seed production, feed formulation and disease management in murrel culture.

Fishing Chimes 30(8): 39-42.

9. Any other information.

Signature of PI / CCPI

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Proforma -1

Developed/ Released/ Adopted Production Technologies/ Innovations

(Page limit: 3 pages/technology)

1. Title of the sub-project: A Value Chain on Murrel Production in Tamilnadu and Orissa

2. Name of CPI: Dr.M.A.Haniffa, Director, Centre for Aquaculture Research and Extension

(CARE), St. Xavier‟s College, Palayamkottai

3. Title of the technology: Live fish Transport Technology

4. Information on existing farming systems, practices, productivity levels and income in the

target area:

Murrels can be kept alive for several hours outside water under slight moist condition

which facilitates transportation to distant markets in good condition. Even then often fishermen

meet heavy loss due to death of murrels during transport. Hence, sedation or use of anesthetics

can be beneficial to calm excitable fish during bulk transportation of fish stocks, especially over

long distances and high density. Practices that require fish handling are a common source of

stress in aquaculture operations and research activities. Hence, a variety of anesthetics are used

mainly in order to reduce stress level and to prevent fish injury/ death during their handling.

5. Key Intervention(s) introduced:

Clove oil is used as anesthetic for live transport of C. striatus

6. Results

All the fish exposed to different concentrations of clove oil recovered well and returned

to normal behaviour with respect to feeding, surfacing activity, swimming and respond to

external stimuli after the anesthetic treatment Furthermore, no mortality was noticed within 48

hrs following recovery from anesthesia. Induction of anesthesia was assumed to be complete

when the fish lost its response to external stimuli. As per the results obtained, the optimum

concentration to anesthetize C. striatus was found to be 450 ppm.

7. Brief description of technology for release:

Increase in clove oil concentration resulted in decrease in induction time whereas the

recovery time increased significantly. However, the recovery and induction time was also

concentration dependent. The mean induction time for C. striatus 400, 450 & 500 ppm clove

oil was found to be 7.10 ± 1.10 min., 2.36 ± 0.42 min. and 2.02±0.52 min. respectively. The

mean recovery time for each

concentration was 5.21±1.1

min., 3.20±0.40 min. and

6.38±1.15 min. respectively.

The induction time was longer

at the lower dose whereas it is

quick and short at higher dose.

In the same manner, the

recovery time was quick in

lower dose whereas it elapsed

for a longer duration at higher

dose.

Time in

minutes

Concentration in ppm

Fig 15 Effect of Clove oil on Induction and Recovery time of C. striatus

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When the fish were put into induction tray containing clove oil, they became excited

and hypermotile followed by bubbling; the gill and fin movements progressively decreased, the

fish lost equilibrium and started swimming laterally. Finally the fish became immobile with full

loss of equilibrium and consciousness. After transfer to recovery tray reappearance of gill

movement was noticed first. This was followed by fin and then tail movement. The fish started

moving laterally. Gradually full equilibrium was regained and normal behaviour was restored

at 5.21±1.1 min., 3.20±0.40 min. and 6.38±1.15 min. as a function of 400 ppm, 450 ppm & 500

ppm clove oil respectively. Erythrocyte count (T/L), Hemoglobin and Hematocrit values

showed elevated levels when compared to control. RBC, Hb & Ht values significantly

increased 1 hr after anesthesia and returned to normal after 24 hrs. Anesthetic treated fishes

exhibited marked decrease in WBCs when compared to control group. The rest of the indices

(MCV, MCH, MCHC, Lymphocytes, Monocytes, Neutrophils) were at comparable levels in all

groups. The anesthetic treated fishes were found to show a significant increase in the

concentration of glucose. The rest of the indices (TP, ALB, GLOB, ALT, AST) were at

comparable levels in all groups. Results of the study suggested that the use of clove oil at the

concentrations of 400, 450 and 500 ppm does not cause irreversible damage on the blood

parameters as well as biochemical profile in C. striatus.

8. Expected Outcome/Impact of the technology:

Anesthetics are useful to fish farmers for the transport of adults as well as fingerlings.

The stress can be reduced to a greater extent and also the economic loss of fish farmers can be

reduced by preventing the fish mortality.

9. Whether findings have been published? If so, give the citation and enclose copy of the

publication.

Research Articles:

5.2 Haniffa MA, Jeya Sheela P, Kavitha K. (2012) Anesthetic Efficacy of Clove Oil and its

Impact on Hematological and Biochemical Changes in Channa striatus (Bloch, 1793)

(Communicated to Turkish journal of Fisheries and Aquatic Sciences IF-0.7).

M.Sc. Dissertation:

5.3 Chandrasekar B. 2012. „Effect of Clove oil on Hematology of Channa striatus challenged

with Aeromonos hydrophila.‟ M Sc thesis, Jamal Mohamed College (Autonomous),

Thiruchirapalli, Tamil Nadu, p 40.

10. Any other information.

Nil

Signature of CPI/CCPI

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Proforma -2

Validation of Developed/ Released/ Adopted Processing Technologies/ Innovations

(Page limit: 3 pages/technology)

1. Title of the Sub-project: A Value Chain on Murrel Production in Tamilnadu and

Orissa (F.No.1(5)/2007-NAIP dt. 22 August, 2008).

2. Name of CPI/CCPI:

CPI:Dr.M.A.Haniffa, St.Xavier‟s College (Autonomous), Palayamkottai – 627 002.

CCPIs:Dr.A.K.Sahu, Dr.Kuldeep Kumar & Dr.Rajesh Kumar, CIFA,Bhubaneswar.

3. Title of the Technology: Murrel Filleting Technology

4. Information on Existing Farming Systems, Practices, Productivity Levels and Income in

the Target Area:

All over India, murrels are sold as live. Dead murrels are not preferred by consumers

and hence fish vendors quite often meet heavy loss. Hence preservation by some kind of

technology is essential. Filleting technology, for boneless preparation of murrels is the opt

one to meet the need. This technology is existing in places like Gazhipur market, New Delhi,

but it is not spread to markets of other states especially

Tamilnadu, Kerala and Karnataka. In Andhra Pradesh now only

the technique is being popularized. For the first time the research

team of CARE has succeeded in preparation of fillet from live as

well as frozen murrel. But the fillet has not gained much attention

among consumers and fish vendors of South India. This

technology is promoted among housewives, working women,

SHGs, star hotels and restaurants.

This technology was tested and fillet was used for preparation of more than 20 recipes

by us. Moreover, 5 CDs highlighting the preparation of cooked and canned products using

fillets have been developed. Filleting technology has very good

scope for preserving dead murrels in frozen form to prepare fillet.

Moreover by this technology, the fish vendors can avoid monitory

loss since dead murrels are not preferred by consumers. The

SHGs, housewives and star hotels can adopt this processing

technology to prepare several fast food items as less time

consuming. Moreover, murrels have medicinal value and are

recommended for convalescence and arthritis patients. Both

CARE and CIFA demonstrated fillet preparation and preparation

of fast food and canned items to the target groups. At present fish

vendors of Gazhipur market, New Delhi are selling 3000 kg/day

of murrel fillet to star hotels and maximum murrel fillet sale is usually during October

(10,000 kg/day) and February (15,000 kg/day).One kilogram of murrel fillet is sold at a rate

of Rs.500 nowadays.

Murrel Fillet

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3. Key Intervention(s) introduced:

Food Products:

Value added products viz., Murrel Fry, Murrel Gravy, Murrel Briyani, Murrel Vermicelli,

Murrel Pollichathu, Murrel Cutlet, Murrel Pickle, Murrel Soup, Murrel Curry, Murrel Poori,

Murrel Finger chips, Murrel Papad, Murrel Noodles, Murrel Fish Soup Powder, Murrel

Shawarma, Murrel Gelatin, Murrel Spring Roll, Murrel Salad, Murrel Tikka, Crispy Murrel,

Murrel Ghatti rolls, Murrel Puffs, Southern Fried Murrel, Murrel Cookies, Murrel Dhall

powder, Muurel Bonda, Murrel Bajji, Murrel Fish Flakes, Murrel Vadai, Murrel Sticks and

Murrel Stew have been prepared. Ready to eat products viz: Murrel Wheat Kakhara, Murrel

Ragi Kakhara, Murrel Rice Kakhara, Murrel Pasta and Murrel Idli and Dosa have been

prepared.

Murrel Fish By-Products:

Gelatin was prepared from the skin of C. striatus, C. marulius, and C. micropeltes.

Fish Oil Essence and Murrel Protein Concentrate were prepared from C. striatus, C.

marulius, and C. micropeltes flesh

6 CDs highlighting their preparations have been developed.

Development of murrel gel and its application in making Papadum and Noodles

Washed pieces of murrel flesh were converted into gel by dilute acetic acid treatment.

Murrel flesh has excellent gel forming ability, emulsification and foaming properties. Murrel

gel has excellent functional properties. It has excellent keeping quality of 3 days at ambient

temperature (300C), 3 months under chilled condition and more than 6 months under frozen

condition. Murrel gel is highly nutritious and can be available on affordable price. Therefore,

we have found its application in development noodles and papadum.

First Patent has been filed for “Murrel Cookies” which is rich in protein and

Polyunsaturated fatty acids (Per serving of Murrel cookies contain: Protein: 0.65 g; Calories:

53; Fat: 3.47g; Carbohydrates: 7.34g). The second patent on “Murrel Dhall Powder” a low

fat food (Fat: 6.04%) and rich in calcium (800.8 mg/100g) and iron (229.5 mg/100g) was

filed on 25.10.2013.

In addition to the above value added products, a combination of Murrel and Millet as

Murrel Millet Mix is something unique to improve health status. Research Collaboration has

been established with Department of Food Science and Nutrition, University of Agricultural

Sciences, GKVK Campus, Bangalore to file patent for murrel millet mix.

4. Results

Status of dissemination/commercialization; and, extent of adoption and success, if

applicable; with supporting data (with tables and photographs as annexure):

For the first time the research team of CARE succeeded in preparation of fillet from live

as well as frozen murrel. Filleting technology yields 40% fillet, 23% head, 22% viscera, 8%

skin and 7% bone and the cost of fillet is Rs.800/Kg. Murrels are usually preferred as live fish

and this technology has a very good scope for preserving dead murrel in frozen form to prepare

fillet. Dead murrels can be stored in ice boxes for 7 days whereas fillet can be stored upto 30

days in freezer (-4ºC). The beneficiaries are SHGs, Housewives and Star Hotels who can adopt

this processing technology to prepare several fast food items. Both CARE and CIFA

f

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96

demonstrated fillet preparation, preparation of fast food items and canned items to the target

groups.

Since the fillets are spine – free, children also show much interest in tasting the fish, thus

providing them with a protein rich diet. The prevailing compounds in murrel fillet were found

as 9-Octadecenoic acid (Z)-, methyl ester (56.69%), Hexadecanoic acid, methyl ester (21.02%),

Octadecanoic acid, methyl ester (10.74%), Arachidonic acid methyl ester (1.87%) and 9-

Hexadecenoic acid, methyl ester, (Z) (2.84%). The other compounds include Dodecanoic acid,

methyl ester (0.82%), Tetradecanoic acid (0.64%), 9,12,15-Octadecatrienoic acid, Methyl Ester,

(Z,Z,Z)(0.56%), Cyclopropaneoctanoic acid, 2-hexyl-, methyl ester(0.68%), Eicosanoic acid,

methyl ester (0.28%) and 8,11,14-Eicosatrienoic acid, (Z,Z,Z)(0.61%). The PUFA/ Saturated

(P/S) ratio of C. striatus fish oil is 0.69 which is higher than the Patin (Pangasius

hypothalamus) with 0.67. On the other hand, the ω-3/ ω-6 ratio of C. striatus is 0.96 which is

higher than the catfish, Clarias macrocephalus (0.25) and Patin (0.73).

5. Brief description of technology for release:

The fish production and consumption patterns have changed over the last 30–40 years, with

both production and consumption being predominant in developing countries. Fish and all

aquatic products are easily digested, and though perishable, are easily processed into various

forms avoiding wastage. Most importantly, fish constitutes one of the main animal protein

sources of the developing world, containing all essential amino acids, thereby providing an

affordable nutrient source to most rural and those below poverty line communities.

Murrel Fish provides an excellent source of essential fatty acids. Murrels also provide

essential micronutrients in the form of vitamins, minerals and co-enzymes. Increasing quantum

of evidence is becoming available on the health benefits of fish consumption, with clear evidence

being brought forward with regard to its impact on common diseases such as cardio vascular

related ones. It is in this respect that there is an increase in fish consumption in the developed

world, whereas in the developing world, in all probability, the driving forces with regard to

increased consumption are its affordability and availability. There is clear evidence that both in

the developing and developed world, fish consumption are on the increase.

High perishability of fish and fish products is a serious problem in tropical countries like India

due to prevailing climatic conditions. Therefore, imparting value addition to this fish will not

only play an important role in developing self-stable products but also in employment generation

and income enhancement of the poor farmers. The entrepreneurs and SHGs, especially women

SHGs and private farmers have been given training on development of different murrel value

added products.

6. Expected Outcome/Impact of the technology:

(8.1) Expected increase in area, production and net income

We have established tie up with Mr.Vinod Wasan, Fish n fun, Gazipur market, New Delhi.

(8.2) Others

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97

7. Whether findings have been published? If so, give the citation and enclose copy of the

publication.

Research Articles

Sahu B B, Kumar K, Sahu A K, Kumar R, Mohanty U L, Jahan N, Sahoo M and Eknath A E.

2012. Carcass Characteristics of Marketable size Striped Murrel Channa striatus (Bloch,

1793). Journal of Applied Ichthyology 28(2): 258-260.

Sahu B B, Kumar K, Sahoo A K, Kumar R, Mohanty U L, Majhi U J, Jahan, N, Sahoo M and

Jayasankar P. 2012. Quality and storage stability of low acid murrel (Channa striatus) pickle

at room temperature. International Food Research Journal 19(4): 1629-1632.

Popular Articles

Haniffa M A, Shanthi P, Kavitha K, Manikandaraja D and Jeya Sheela P. 2013. Value

Addition of Murrels: a Boon to Fish Farmers. Fishing Chimes

Sahu B B, Kumar K, Sahu A K, Mohanty U L, Kumar R, Sahoo M, Jahan N and Eknath A E,

2011. Processing and value addition to murrel in value chain. Fishing Chimes 31(1): 106-

108.

Haniffa M A and Mohamed Riyaz H. 2012. Murrel Fish Food Expo 2012. Fishing Chimes

32(5): 9-12

Haniffa M A, Jeyasheela P, Manikandaraja D, Jafar Sathik S, and Bilal Ahmad. 2013. Nellai

Fish Food Festival 2013. Fishing Chimes 33(6): 18-24

Haniffa M A, Jeyasheela P, Manikandaraja D, Jafar Sathik S, and Bilal Ahmad. 2013. Nanjil

Food Fish Festival 2013. Fishing Chimes 33(6): 25-28.

Haniffa M A. 2013. Mana Aluthama, Viral Meen Biscuit Sapidunga (Tamil) (Murrel biscuits

a cure for depression). Kungumam, 26.08.2013, p 95-96.

8. Any other information.

Signature of PI / CCPI

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Proforma -2

Processing Technologies/ Innovations

1. Title of the sub-project: A Value Chain on Murrel Production in Tamil Nadu and Orissa

2. Name of CPI: Dr.M.A.Haniffa, Director, Centre for Aquaculture Research and

Extension (CARE), St. Xavier‟s College, Palayamkottai

3. Title of the technology: Human Health Management Technology

4. Information on existing farming systems, practices, productivity levels and income in

the target area:

Channid namely Channa striatus are economically important not only for its nutritional

value but also for its putative effects on wound healing, arthritis, ulcer, convalescence and

inflammation. In Malaysia, C. striatus is consumed as protein source to induce wound healing

after child birth and now becoming supplementary among caesarean mothers and illnesses such

as cancer and diabetic gangrene. In the present investigation, this species was analyzed for its

wound healing effects on male wistar rats.

5. Key Intervention(s) introduced:

Improvement of human health status by consuming C. striatus

6. Results (Status of dissemination/commercialization; and, extent of adoption and

success, if applicable; with supporting data (with tables and photographs as annexure)):

The muscle tissues of four Channa species namely, Channa striatus, C. punctatus, C.

marulius and C. diplogramme were screened against five enteric pathogens namely Salmonella

typhi, Staphylococcus aureus, Klebsiella vulgaris, Shigella dysenteriae and Shigella boydii. A

maximum zone of inhibition (24.6 mm) was observed in C. striatus against Shigella dysenteriae.

It is therefore suggested that C. striatus can be a potential source of an antimicrobial protein for

specific human pathogens.

The antibacterial activity of skin mucus and intestinal mucus of five different Channa sp

viz Channa striatus, C. micropeltes, C. marulius, C. punctatus and C. gachua were tested against

five pathogenic bacteria namely Aeromonas hydrophila, Pseudomonas aeruginosa, Escherichia

coli, Vibrio fischeri and V. anguillarum. Maximum antibacterial activity was observed against

P. aeruginosa for the skin mucus (28±2.9mm) and intestinal mucus (29±3.2 mm) respectively,

suggesting the presence of antimicrobial peptides /proteins in fish mucus that makes the

membrane to act as barrier.

The fatty acids present in the murrel fillet were studied. The prevailing compounds in

murrel fillet were found as 9-Octadecenoic acid (Z)-, methyl ester (56.69%), Hexadecanoic acid,

methyl ester (21.02%), Octadecanoic acid, methyl ester (10.74%), Arachidonic acid methyl ester

(1.87%) and 9-Hexadecenoic acid, methyl ester, (Z) (2.84%). The other compounds include

Dodecanoic acid, methyl ester (0.82%), Tetradecanoic acid (0.64%), 9,12,15-Octadecatrienoic

acid, Methyl Ester, (Z,Z,Z)(0.56%), Cyclopropaneoctanoic acid, 2-hexyl-, methyl ester(0.68%),

Eicosanoic acid, methyl ester (0.28%) and 8,11,14-Eicosatrienoic acid, (Z,Z,Z)(0.61%).

Wound healing studies revealed faster healing upon topical application of C. striatus

crude extract by 21st day in 10% concentration whereas, 100% healing was observed on 18th

day

for standard treatment. Regarding anti-inflammatory studies, the striped murrel extract at

400mg/kg dose showed significant decrease in the paw volume (65.59%) of wistar rats when

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compared to the control Indomethacin. In anti-arthritis studies, C. marulius rendered about

91.72% healing whereas C. striatus crude extract rendered 88.43% healing in comparison with

the Standard drug (92.86%). The results of our studies revealed that murrels are bio-assets to the

country and should be conserved for future generation.

7. Brief description of technology for release:

Since the fillets are spine – free, children also show much interest in tasting the fish, thus

providing them with a protein rich diet. Moreover, the PUFA/ Saturated (P/S) ratio of C. striatus

fish oil is 0.69 which is higher than the Patin (Pangasius hypothalamus) with 0.67. On the other

hand, the ω-3/ ω-6 ratio of C. striatus is 0.96 which is higher than the catfish, Clarias

macrocephalus (0.25) and Patin (0.73).Murrels have medicinal value and are recommended for

convalescence and arthritis patients. In South-east Asian countries (Malaysia, Singapore,

Vietnam, Thailand, etc.), the murrel fish oil essence is commercially available and ingested by

ladies after delivery for their post-operative wound healing effects.

8. Expected Outcome/Impact of the technology: Not immediate

(8.1) Expected increase in area, production and net income : NA

(8.2) Others: NA

9. Whether findings have been published? If so, give the citation and enclose copy of the

publication.

Research Articles:

Dhanaraj M, Haniffa M A, Arunsingh V, Muthu Ramakrishnan C, Manikandaraja D and

James Milton M. 2009. Antibacterial activity of skin and intestinal mucus of five different

murrel species. Malaysian Journal of Science 28(3): 257-262.

Haniffa M A, Jeya Sheela P and James Milton M. In vitro antibacterial activity of tissue

extracts from four channids against enteric pathogens. Journal of Agricultural Technology

9(6): 1437-1445.

Haniffa M A, Jeyasheela P, Kavitha K, Mat Jais A M. 2013. Salutary values of striped

snakehead- Channa striatus – A review. Asian Pacific Journal of Tropical Biomedicine

(Accepted for publication).

10. Any other information: Nil

Signature of CPI/CCPI

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Proforma -3

Details of Commercialized Technologies/ Innovations

(Page limit: 3 pages/technology)

1. Title of the Sub-project: A Value Chain on Murrel Production in Tamilnadu and Orissa

(F.No.1(5)/2007-NAIP dt. 22 August, 2008).

2. Name of CPI/CCPI:

CPI : Dr.M.A.Haniffa, St.Xavier‟s College (Autonomous), Palayamkottai – 627 002.

CCPIs: Dr.A.K.Sahu, Dr.Kuldeep Kumar & Dr.Rajesh Kumar, CIFA, Bhubaneswar.

3. Title of the Technology: Murrel Pickle

4. Commercialization status with date of licensing/MOU:

We have established tie up with Arasan supermarket, Palayamkottai for the commercialization

of murrel pickle. For this purpose, murrel pickle was evaluated after an elapse of seven days on

changes in chemical and microbiological properties at an interval of 15 days upto 60 days of

storage. After 60 days of storage period microbiological count and quality traits did not show

appreciable change and remained satisfactory throughout the storage period. Now the product is

ready for commercialization. Tie up has been established with SHGs for commercialization of

murrel pickle for their income generation.

5. Brief description of intervention / innovation:

Change in murrel farmer‟s perceptions towards modern technology is the present need to

enable the farmers to understand the principles and benefits of new production systems and

practices. Such changes can only be done through good demonstration of the advantages of the

new and advanced systems/practices.

Emphasis is being given on developing self-stable fish

and fish food products, which can be stored at ambient

temperature (32 + 0.50C). Fish pickling in vinegar and edible

oil with added salt, spices and condiments provide ready to eat

highly acceptable convenience product with good self-stability

at an ambient temperature. Low moisture and reduced pH are

the important major factors contributing to the self-stability of

the pickles. Pickling also helps in improving the desirable

characteristics like taste and flavor along with the preservative

effect. The preliminary trials and evaluation of market fish pickle has indicated that, with pH

range 4.4 - 4.7, the products are objectionably sour; consequently its acceptability was decreased.

Information on development and storage stability of low acid murrel pickle is limited. Therefore

the objective of the present study was to prepare highly acceptable low acid murrel pickle and

study their storage stability at 32 +0.50C.

Low acid murrel meat pickle was prepared using deboned meat from marketable size

murrel (700 + 59 g). The product was evaluated after an elapse of seven days on maturation for

change in physico-chemical, microbiological and organoleptic properties at an interval of 15

days up to 60 days at 32 + 0.50C. Results showed that pH and titratable acidity of the low acid

pickle was 4.83 and 0.68, whereas in control murrel pickle, these values were 4.68 and 0.74

respectively. After 60 days of storage period microbiological count and sensory quality traits did

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101

not show appreciable change and remained satisfactory throughout the storage period. Low acid

pickles had significantly lower sourness and high overall acceptability compared to the control.

Murrel pickle has the potential of becoming ready-to-eat, self-stable, highly acceptable health

food products of indigenous origin.

Name and address of the firm(s) which links to commercialized the product:

1. Arasan Supermarket,

Kamatchi Amman Kovil street, 1st cross street, KTC Nagar, Tirunelveli – 627 011.

2. Aryaas Super market

139 A, Trivandrum road, Palayamkottai-627002

Contact number: 0462 2585713, 2585450.

3. Anantha Departmental Stores

81 B/1&2,Tiruchendur Road, Palayamkottai-627002

Contact number: 0462 2585801, 2585802

6. Area (state(s)/district(s)) covered:

Tie up with different supermarkets of Tamil Nadu is under process.

7. Volume/quantity of Annual production and approximate sale value:

8. Benchmark (existing similar product) and Consumer acceptance, particularly in case of

food products:

9. Status of patenting, if patentable, trademark or any other IPR title, if applicable

Signature of CPI/CCPI

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102

Pro-forma 3

Details on Rural Entrepreneurships/ Rural Industries Developed

(Page limit: 3 pages/ rural industry)

1) Title of the sub-project:

2) Name of CPI:

3) Name of rural industry with address:

4) Contact: Phone and E-mail of rural industry:

5) Investment (Rs): NAIP Funds

Industry/ Entrepreneur

6) Product(s) produced and marked:

7) Annual Production (kg or litre):

8) Raw Material(s) and Quantity used/ year (kg or litre):

9) Cost of raw material (per kg or litre):

10) Price of Product: In Whole Sale

In Retail

11) Type of Beneficiaries:

12) Number of Beneficiaries:

13) How the Industry is beneficial to primary producers:

14) Estimate Employment Generation/ Year (person days):

15) CPI to explain whether the industry is approved by FPO/BIS or any other statutory body and

how the food safety and quality assurance of end product are being ensured?

Note: Use separate pro-forma for each entrepreneurship/ rural industry

Attach photograph(s) relevant to the industry/ entrepreneurship

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103

Proforma -4

Information on Technologies/ Innovations Filed for Patent

1. Exact title of the technology and date of filing application: Nutritional Cookie

Composition And A Process For Preparing The Same

2. Where it was filed?

The Patent application was filed by “Metayage IP Strategy Consulting LLP‟, Coimbatore

3. Present status (if number is awarded, give No. and date):

Patent filing Specifications: 1277/DEL/2013 dt.30.04.2013

4. Brief write up of the technology(duly masking the IPR related issues) which can be

printed:

Murrel Cookie is an edible food product rich in protein and Poly unsaturated fatty acids (Per

serving of Murrel cookies contain: Protein: 13.65%; Energy 471.1Kcal/100g; Fat: 10.1%;

Carbohydrates: 63.04%; Calcium 0.45%; Iron 5.5mg/100g).

As it is high in nutrition, it can be consumed as snacks to meet immediate energy

requirement of all age groups, since it is more nutritious than other cookies.

The protein content of the Murrel cookie is higher than the cookies available in the markets.

Murrels are rich in Omega-3 (EPA – 0.21% and DHA- 0.19%) fatty acids. Hence, no doubt

that the incorporation of Murrel fish in cookie will improve the health of the consumer.

Preparation is easy and increased storage time and no fishy odour are added advantages

5. Whether the technology is commercialized?

No. Tie up has been established with Millenium Exports, Periamet, Chennai and

Mr.Sukumar, Thureya Properties, Coimbatore for Commercialization.

6. Impact of the technology:

Since the murrel cookie is rich in nutrients, malnourished and old age people will be

benefitted. Murrel cookies will definitely play an important role in improving the health status of

the socially weaker sector.

Signature of CPI/CCPI

Murrel Cookies

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104

Proforma -4

Information on Technologies/ Innovations Filed for Patent

1. Exact title of the technology and date of filing application: Process For Preparaing

Nutritionally Rich Dhal Powder And Its Composition

2. Where it was filed?

The Patent application was filed by “Metayage IP Strategy Consulting LLP‟, Coimbatore

3. Present status (if number is awarded, give No. and date):

Patent filing Specifications: 3168 /DEL/2013 dt.25.10.2013

4. Brief write up of the technology(duly masking the IPR related issues) which can be

printed:

Murrel Dhal Powder was prepared due to its richness in Omega 3 (EPA – 0.21% and DHA-

0.19%) fatty acids (in murrel) for promoting growth of malnutrition babies as well as old people.

A combination of Murrel and Dhal together as Murrel Dhal powder is something unique to

improve health status of school children and aged people. Increase in the concentration of murrel

fish powder resulted in decrease in fat and increase in calcium in the murrel dhal powder. The

Murrel Dhal Powder is a low fat food but enriched with calcium as details given below:

S. No Chemical Composition % of Channa striatus

0 20 30

1 Fat (g/100g) 13.56 7.31 6.04

2 Calcium (mg/ 100g) 403.2 603.3 800.8

5. Whether the technology is commercialized?

No. Tie up has been established with Arasan Super Market, Tirunelveli for

Commercialization.

6. Impact of the technology:

Since the murrel dhall powder is a low fat food rich in calcium, malnourished, diabetic

and old age people will be benefitted. Murrel dhall powder will definitely play an important role

in improving the health status of the socially weaker sector.

Signature of CPI/CCPI

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Value Chain Flow Diagram as Approved by PMC

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A VALUE CHAIN ON MURREL PRODUCTION IN TAMIL NADU AND ORISSA (As per the original proposal approved by the PMC)

OUTPUT

**Publications: IF: Journals with Impact Factor; PR: Peer Reviewed Journals; PA: Popular Articles; Proc: Proceedings; Tech Bull: Technical Bulletin;

CP: Communicated Papers

PR

OD

UC

TIO

N T

EC

HN

OL

OG

Y

R

O

D

C

T I O

N

A.S

EE

DL

ING

S

B.G

RO

W O

UT

Mass Seed

Production

Mass Culture

of Live Feed

Pelleted feed

for murrels

Larviculture

of Murrels

Murrel Growout

culture

Live Fish

Transport

Disease

Management

*4,81,147 seeds of C. striatus & C. marulius produced

*8,000 – 12,000 eggs produced by HCG 2000 IU / Kg female PR: 2; PA: 5; CDS:

4, Book Chapter: 3

CP: 1; Proc: 1

*Cladocerans (8,000/l), Copepods (7,000/l), Rotifers

(10,000/l) produced

*Succeeded in mass culture of zooplankton, Vermiculture

and Tubifex

PR:4; Book: 4;

CDS: 1

Ph.D Thesis: 1

Ann: I

Plate: 1

*Diet containing fish meal (anchovy and jawala) – 60%,

wheat flour – 10%, rice flour - 10% and soya flour 20%

with herbs and probiotics formulated

Ann: I

Plate: 2

PR:1; CP:1

CDS: 1

Ann: I

Plate: 3

*3 days old post larvae rotifers suitable for 15 days

*16th

day onwards with boiled chicken intestine

*30th

day onwards- semi- moist or pellet feed

recommended *Earthern Ponds of 0.1 ha to 0.4 ha with a depth of 1m

suitable for culture

*52 farmers in Tamil Nadu harvested 27.67 tons ranging

from 0.1 ton – 5 tons/ha/yr with a profit range of Rs.30,000

to Rs. 15,00,000/ha/yr in large scale

*1119 farmers were trained through 21 training and twelve

workshop in Tamil Nadu,, Karnataka, Andhra Pradesh and

Orissa

*Recommended dosage for 48 hrs transport- 450 ppm

clove oil

*Aluminium drums (1.75*2*6 ft) of 100 l capacity

suggested for transport of 45 adult murrels (25 Kg) for 24

hours travel

Herbal pastes were prepared using medicinal herbs Coleus

aromaticus, Wrightia tinctoria, Azadiracta indica and

turmeric for treating EUS of murrels.

IF:2; PR:6; CP:1;

Proc:5, MSc.

Theses: 4

Ph.D Theses: 3

Ann: I

Plate: 3

PR:1; PA:13;

Proc:7; CDS: 8

Books: 3

Book Chapter: 7 Tech Bulletin: 6

Ann: I

Plate: 4

CP:1

M.Sc. Thesis:1

Ann: I

IF:2; PR:5; PA:1;

Proc:6; Ph.D

Thesis:1

M.Sc.Theses:5

Ann: I

Plate: 5

Annexure &

Photo plates Publications**

*

OUTPUT November 2008 – March 2014 Interventions Missing Links

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**Publications: IF: Journals with Impact Factor; PR: Peer Reviewed Journals; PA: Popular Articles; Proc: Proceedings; Tech Bull: Technical Bulletin;

CP: Communicated Papers

PR

OC

ES

SIN

G

R

O

D

C

T I O

N

MA

RK

ET

ING

R

O

D

C

T I O

N

DIS

SE

MIN

AT

ION

R

O

D

C

T I O

N

*Filleting technology yielded 40% fillet, 23% head, 22% viscera,

8% skin and 7% bone and the cost of fillet is Rs.800/Kg

* Fillet can be stored upto 30 days in freezer (-4 ºC)

*Murrel fillet used for preaparation of Murrel fry, Murrel gravy,

Murrel Briyani, Murrel Vermicelli, Murrel Pollichathu, Murrel

Cutlet, Murrel Pickle, Murrel Soup, Murrel Curry, Murrel Poori,

Murrel Finger chips, Murrel papad, Murrel Noodles, Murrel fish

Soup Powder, Murrel Shawarma, Murrel Gelatin, Murrel spring

roll, Murrel salad, Murrel Tikka, Crispy murrel, Murrel Ghatti

rolls, Murrel Puffs, Southern fried murrel, Murrel Cookies, Murrel

Dhall powder, Murrel Bonda, Murrel Bajji, Murrel Fish Flakes,

Murrel Vadai

*PATENT has been filed for “Murrel Cookies” on 30.04.2013 and

Murrel Dhall Powder on 25.10.2013

Next Patent on Murrel Millet Mix is under progress

Murrel Fillet –

Value Addition IF:1; PR:3, PA:6;

CP:4, Proc:1;

Tech Bull:2

Ann: II

Plate: 5-7

Linkages

* Linkages have been developed with fish vendors in Tamil Nadu,

Kerala, Karnataka and Andhra Pradesh and different states

department fisheries, KVKs, NFDB and State Fisheries Colleges

*We have included SHGs (Women and Men) for murrel farming

* Linkages have been developed with Manian Laboratories,

Coimbatore, Bharathiyar University, Coimbatore, University of

Agricultural Sciences, Bangalore, Prathyusha Institute of

Technology, Chennai, Scott Christian College, Nagercoil, Malar

Magalir Self Help Group, Nagercoil, Central Institute of

Brackishwater Aquaculture, Chennai, Aquaculture Foundation of

India, Chennai

NA Ann: III

Plate: 8

Linkages

* Collaboration with fish vendors and fish traders

* Periodical training and workshop in neighbouring states (Kerala,

Karnataka, and Andhra Pradesh) apart from Tamil Nadu

*About 21 training and 12 workshop were organized by CARE,

CIFA and TNC

* Awareness program via media (Daily news papers, technical

manuals, pamphlets, fact sheet etc.) and television

PA: 10

Newspaper: 40

CDs:4

Ann: III

Plate: 8

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Murrel Value Chain Sustainability Backyard hatcheries for small

scale production -10,000 seeds in

an area of 289 sq.ft.

large scale 100000 seeds in 0.5

acre

Confidence in production of live

feed, Semi moist feed and

pelleted feed.

Network of farmers registered;

CART at KIFVI, Kalakad village;

Youtube all videos on murrel culture;

Daily contact by email / Phone and

website

Linkages developed among murrel

farmers, fish vendors, processors and

markets.

Availability of video CDs in youtube under

murrel culture;

Articles on EUS treatment in the website;

Visits by CARE research team to

stakeholders farms.

Impact Stakeholders confident to

produce 100000 seeds in an area

of 0.5 acre

Net Profit Rs.3 Lakhs

Stakeholders confident of

preparing feed; Housewives

expertise in plankton culture at

backyard.

Increase in number of murrel farmers 52

from 0

Rs.100-150/ kg in 2008.

Rs 600-750/kg now Demand more;

supply less; Production / ha =0.1 - 5 tons

Assured market consumer safety for

Export

Consumers preference for murrel fillet

(e.g. Murrel Pickle)

Loss due to dead fish reduced

Shelf life of murrel fillet (for 30 days)

increased

Preparation of ready to cook and ready to

eat products in the absence of marine

fish in markets.

Stakeholders confidence in EUS treatment

Due importance to water quality.

Outcome 2000 IU HCG to produce 8000-

12000 eggs

107889 Seeds produced by SXC

263258 Seeds produced by

CIFA.

110000 Seeds produced by

stakeholders, Orissa

Semi-moist diet (fish meal:

Anchovy and Jawala 60%; Wheat

flour 10%; Rice flour 10% and

Soya flour 20%) recommended -

probiotics and herbs incorporated.

0.1 ha- 0.4 ha pond with 1m depth

recommended; 52 farmers in Tamil Nadu

started Murrel culture harvested 27.67

tons and earned Rs.62,90,650/-;

83 farmers harvested 5.5 tons of murrels

with an income of Rs. 1,23,900/- per 1.4

ton murrel.

Aluminium drums and Anasthetics didn’t

receive attention.

Diseased fishes quarantined and treated

with Coleus aromaticus, Ocimum sanctum,

Curcuma longa and Wrightia tinctoria;

Feed incorporated with Probiotics for

disease resistance

Interventions Refinement of Induced breeding

techniques to produce seeds

throughout the year.

Rotifers (10,000/l) cladocerans

(8000/l), copepods (7000/l)

produced.

Mass culture of zooplankton -

TNC

Vermiculture and tubifex- CIFA

Rotifiers for 3 days old post

larvae

16th day onwards- boiled chicken

intestine

30th day onwards- semi-moist

feed

1. Motivation of stakeholders to start

murrel culture by training, workshop and

Expo

2. Refinement of culture technology.

3. Preparation of semi moist and pelleted

feed.

4.Incorporation of probiotics and herbs

in diets

Filleting: 40% fillet, 23% head, 22%

viscera,8% skin and 7% bone

Cost of fillet Rs.800/ kg. Preparation of

ready to eat and ready to cook products-

Housewives, SHGs, Star hotels etc.,

Murrel soup from head, leather from

Skin and manure from other wastes

Handle live murrel with care –use cloth

To avoid loss of mucus-avoid abrasion

Herbal pastes prepared using Coleus

aromaticus, Wrightia tinctoria, Azadiracta

indica and turmeric for EUS treatment.

Reasons Monsoon failure, Absence of

Natural breeding, lack of

knowledge on Induced breeding

Techniques.

1. Refinement of mass culture of

live feed -Train fingerlings to

feed on pelleted feed

2. Supply of feed as semi-moist

(with or without fishmeal) to fry

and fingerlings

Non availability of readymade feed in

markets.

Non availability of murrel seeds;

Carnivorous, piscivorous and

cannibalistic behaviour of murrels;

Occurrence of EUS.

Aluminium drums of 100 l capacity to

transport 50 adult murrels for 24 hrs.

450ppm clove oil recommended for live

fish transport

Filleting technology popularized.

EUS more during rainy season

More in the wild capture- market samples.

Issues Lack of production /sale centres

for murrel seeds.

Seed availability only seasonal in

the wild

No readymade feed as for carps;

carnivorous, piscivorous and

cannibalistic habit Non

acceptability of pelleted feed and

lack of knowledge on live feed

Lack of interest in murrel culture;

Psychological fear

Consumers preference live murrel

mortality during live murrel transport.

Gut break of Diseases (EUS) resulting in

mass mortality.

Murrel Seed Production Difficulty in larviculture of

murrels

Murrel Growout Culture Dead murrels –Heavy loss to fish

vendors.

Disease Management

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Murrel Value Chain

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Annexure 1

Guidelines for Citation of Publications from NAIP sub-projects

1. Research Article:

1.1 Dhanaraj M, Haniffa M A, Arunsingh V, Muthu Ramakrishnan C, Manikandaraja D and

James Milton M. 2009. Antibacterial activity of skin and intestinal mucus of five different

murrel species. Malaysian Journal of Science 28(3): 257-262.

1.2 Mehraj Ud Din W, Altaff K and Haniffa M A. 2009. Study on the growth and survival of

Channa striatus post larvae (Bloch) using live feed. Bangladesh Journal of Fisheries Science

13(2): 131-135.

1.3 Mehraj Ud Din W, Altaff K and Haniffa M A. 2009. Comparative study on growth of Channa

striatus fry and fingerlings using different feeding regimes. Journal of Aquatic Biology

24(2): 173-176.

1.4 Altaff K and Mehraj Ud Din W. 2010. Culture of Ceriodaphnia cornuta using chicken

manure as fertilizer: Conversion of a Waste product into highly nutritive protein. Pakistan

Journal of Scientific and Industrial Research 53(2): 89-91.

1.5 Mehraj Ud Din W and Altaff K. 2010. Culture of zooplankton for rearing fish larvae.

Pollution Research 29(2): 91-93.

1.6 Mehraj Ud Din W, Altaff K and Haniffa M A. 2010. Evaluation of growth and survival of fry

of Channa striatus (Bloch) using different feeding regimes and cladoceran live feed. Journal

of Experimental Zoology India 13(1): 225-227.

1.7 Mehraj Ud Din War and Kareem Altaff. 2011. High density culture of a cladoceran Moina

micrura (Kurz, 1874) by utilizing poultry manure. Pakistan Journal of Scientific and

Industrial Research 54(3): 131-135.

1.8 Mehraj Ud Din War, Kareem Altaff and Mohammed Abdul Khader Haniffa. 2011. Growth

and survival of larval snakehead Channa striatus (Bloch, 1793) fed different live feed

organisms. Turkish Journal of Fisheries and Aquatic Sciences 11(3):523-528.

1.9 Muthukrishnan Dhanaraj and Mohamed Abdul Kather Haniffa 2011. Effect of probiotics on

growth and microbiological changes in snakehead Channa striatus challenged by Aeromonas

hydrophila. African Journal of Microbiology Research 26(5):4601-4606.

1.10 Rauta P R, Kumar K and Sahoo P K. 2011. Emerging new multi-drug resistant bacterial

pathogen Acinetobacter baumanii from snake head Channa striatus from India. Current

Science 101(4): 548-553.

1.11 Sahu B B, Kumar K, Sahu A K, Kumar R, Mohanty U L, Jahan N, Sahoo M and Eknath

A E. 2012. Carcass Characteristics of Marketable size Striped Murrel Channa striatus

(Bloch, 1793). Journal of Applied Ichthyology 28(2): 258-260.

1.12 Sahu B B, Kumar K, Sahoo A K, Kumar R, Mohanty U L, Majhi U J, Jahan, N, Sahoo M

and Jayasankar P. 2012. Quality and storage stability of low acid murrel (Channa striatus)

pickle at room temperature. International Food Research Journal 19(4): 1629-1632.

1.13 Haniffa M A and Kavitha K. 2012. Antibacterial activity of medicinal herbs against the

fish pathogen Aeromonas hydrophila. Journal of Agricultural Technology 8(1): 205-211.

1.14 Haniffa M Aand Shanthi P. 2012. Phytochemical analysis and antibacterial screening of

medicinal plants against Aeromonas hydrophila. Asian Journal of Pharmaceutical and

Clinical Research

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1.15 Bilal Ahmad Paray, Haniffa M A and Manikandaraja D. 2012. Induced ovulation and

spawning of a striped snakehead murrel Channa striatus (Bloch) under captive conditions.

Journal of Research in Animal Sciences 1(1): 033-039.

1.16 Kuldeep K, Lalrinsanga P L, Sahoo M, Mohanty U L, Kumar R and Sahu A K. 2013.

Length-Weight Relationship and Condition Factor of Anabas testudineus and Channa

Species under Different Culture Systems. World Journal of Fisheries and Marine Science

5(1): 74-78.

1.17 Haniffa M A, Jeya Sheela P, James Milton M and John De Britto A. 2013. Invitro and

Invivo Antimicrobial Effects of Wrightia tinctoria (Roxb.) R. Br. against Epizootic

Ulcerative Syndrome in Channa striatus. International Journal of Pharmacy and

Pharmaceutical sciences 5(3): 219-222.

1.18 Haniffa M A, Kavitha Bharathi B, Viji Margaret I, Bilal Ahmad Paray and Manikandaraja

D. 2013. Effect of probiotics and certain herbal additives on growth, survival and disease

resistance of Channa striatus challenged by Aeromonas hydrophila. World Aquaculture

44(2): 64-67.

1.19 Haniffa M A, Jeya Sheela P and James Milton M. In vitro antibacterial activity of tissue

extracts from four channids against enteric pathogens. Journal of Agricultural Technology

9(6): 1437-1445.

1.20 Ramesh S, Chelladurai G and Haniffa M A. 2013. Isolation of enzyme producing

bacterial from gut of Channa striatus fed on different herbs and probiotics diet. International

Journal Pharmacy and Pharmaceutical Sciences 5(4): 195-198.

1.21 Mehrajuddin War and Kareem Altaff. 2013. Preliminary Studies on the Effect of Prey

Length on Growth, Survival and Cannibalism of Larval Snakehead, Channa striatus (Bloch,

1793). Pakistan Journal of Zoology 45(2): 1-6.

1.22 Bilal Ahmad Paray, Haniffa M A, Manikandaraja D and James Milton M. 2013.

Breeding Behaviour and Parental Care of the Induced Bred Striped Murrel Channa

striatusunder Captive Conditions. Turkish Journal of Fisheries and Aquatic Sciences 13:

707-711.

1.23 Mohapatra S D, Kumar K, Jayasankar P and Barman H K. 2013. Establishment of dry –

down hypoxic stress treatment protocol for snakehead freshwater fish, Channa striatus.

International Journal of Fisheries and Aquatic Sciences 1(2): 36–39.

Papers in Press

1.24 Haniffa M A, Jeya Sheela P, James Milton M and Kavitha K 2012. Protein Profiling for

Phylogenetic Relationship of Snakehead Species. Iranian Journal of Fisheries and Aquatic

Sciences (Accepted)

1.25 Haniffa M A, Jeyasheela P, Kavitha K, Mat Jais A M. 2013. Salutary values of striped

snakehead- Channa striatus – A review. Asian Pacific Journal of Tropical Biomedicine

(Accepted)

1.26 Haniffa M A, Viji Margaret I, Kavitha Bharathi B and Jeya Sheela P. 2013 Dietary

administration of herbal additive Phyllanthus niruri on growth performance and

haematological changes in striped snakehead Channa striatus. World Aquaculture

(Accepted)

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1.27 Mehrajuddin War and Kareem Altaff. Utilization of Poultry excreta for high density

production of Daphnia carinata (King 1853). Cost effective and environmental friendly

technique. Pakistan Journal of Scientific and Industrial Research (Accepted)

1.28 Haniffa M A, Shanthi P, Kavitha K, Manikandaraja D and Jeya Sheela P. 2013. Value

Addition of Murrels: a Boon to Fish Farmers. Fishing Chimes

Communicated papers

1.29 Haniffa M A, Jeya Sheela P, Vigneswaran R and Kavitha K. 2012. Effect of Vitamins on

Digestive Enzyme Activities and Growth Performances of Striped Murrel Channa striatus.

Iranian Journal of Fisheries and Aquatic Sciences

1.30 Haniffa M A, Jeya Sheela P, James Milton M, Kavitha K and Ajaz Ali Bhat. 2012. Inter

simple sequence repeat (ISSR) marker and principal component analysis (PCA) for species

identification and evolutionary analysis. Biochemical Systematics and Ecology

1.31 Haniffa M A, Jeya Sheela P and Kavitha K. 2012. Anesthetic Efficacy of Clove Oil and

its Impact on Hematological and Biochemical Changes in Channa striatus (Bloch, 1793).

Turkish journal of Fisheries and Aquatic Sciences

1.32 Bilal Ahamad Paray, Haniffa M A and Ananth Kumar Y. Studies on Embryonic and

Larval Development of Induced Bred Channa striatus. Envirobiotech Journal.

1.33 Ajaz Ali Bhat, Haniffa M A, James Milton M, Bilal Ahmad,

Divya P R, Gopalakrishnan

A. 2013. Genetic variation of Striped Snakehead (Channa striatus, Bloch, 1793) populations

using RAPD markers. International Journal of Biodiversity and Conservation

2. Book

2.1. Sundararaj V and Haniffa M A. 2010. Veral Valarppu, p 248 Naveena Velaanmai, Chennai,

India.

2.2. Haniffa M A. 2011. Aquatic Resources and Aquaculture, p 328 Dominant Publishers and

Distributors Pvt. Ltd., New Delhi, India

2.3. Sundararaj V. and Altaff K. 2012. Meen Valarpil Uyirunavugal, p 258 Naveena Velanmei,

Chennai, India.

2.4. K. Kumar, R. Kumar, S. Saurabh, M. Sahoo, A. K. Mohanty, P. Lalrinsanga, U. L. Mohanty,

A. K. Sahu and P. Jayasankar. 2012. Snakehead: Fishes fact sheets. p 38 Central Institute of

Freshwater Aquaculture (ICAR), Kausalyaganga, Bhubaneswar.

3. Book Chapter

3.1.Eknath A E, Kumar K, Jena J K, Mohanty U L, Kumar R, Jahan N and Sahoo M. 2010.

Murrel: An ideal fish for mass production (in) souvenir of Assam Matsya Mahotsav,

organized by the Department of Fisheries, Govt. of Assam during 30th

Jan- 1st Feb 2010. p 5-

8

3.2. Kumar K, Sahu A K, Kumar R, Mohanty U L, Sahoo M and Jahan, N. 2011. Murrel Culture

in Odisha-A Humble Beginning (in) Aquaculture Innovators, p 105-112, Radheshyam et al.

(Eds), Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, India.

3.3. Kumar K, Kumar R, Mohanty U L and Sahu A K. 2012. Murrel culture and its

popularization. (in) Training manual on Fish Breeding and Seed Production, p37-45, KVK,

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CIFA (Eds), Department of Fisheries, Govt. of Odisha and KVK- Khurda, Central Institute

of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, India.

3.4 Kumar R, Kumar K, Mohanty U L and Sahu A K. 2012. Murrel (Channa striatus): Potential

candidate species for nutritional security and livelihood generation. (in) Souvenir: The world

food day, p 49-51, S J Swain et al. (Eds), Odisha Krishak Samaj, Bhubaneswar, Odisha.

3.5 Kumar K, Kumar R, Mohanty U L, Saurabh S, Sahu B B, Sahoo M, Mohanty A, Sahu A K

and Jayasankar P. 2013. Snakehead fishes: Alternative candidate fish for diversification

aquaculture. pp- 119-132 (in) Aquaculture: New Possibilities and Concerns, P-187 Sinha,

V.R.P., Jayasankar, P. (Eds), Narendra Publishing House, Delhi, India.

3.6 Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) Ka beej utpadan ebm Palan

(Hindi) (Seed Productuction and culture of murrel) (in) Training manual on Jalkrishi mein

Dakhata Vikash, p 50-54. Published by Director, CIFA, Kausalyaganga, Bhubaneswar, India

on 15-19 July 2013.

3.7 Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) Ka beej utpadan ebm Palan

(Hindi) (Seed Productuction and culture of murrel) (in) Training manual on Unnat mithajal

matya palan, p 54-57. Published by Director, CIFA, Kausalyaganga, Bhubaneswar, India.

12-16 December 2013.

3.8 Kumar R, Mohanty U L and Verma D K.2013. Saoul (murrel) ka beej utpadan ebm Palan

(Hindi) (Seed Productuction and culture of murrel) (in) Training manual on ‘Mithajal Matya

palan mein unnat praudhogiki’, p56-59. Published by Director, CIFA, Kausalyaganga,

Bhubaneswar, India. 20-24 December 2013.

4. Thesis:

4.1.Mohamed Ansari A. 2009. ‘Effect of water probiotics on intestinal microflora and growth,

Aeromonas hydrophila challenged Channa striatus exposed to different salinity.’ M Sc

thesis, Jamal Mohamed College (Autonomous), Thiruchirapalli, Tamil Nadu, p 55.

4.2. Jeyakumar R. 2010. ‘Studies on the effect of herbal concoction in the therapy of Channa

striatus (Bloch).’ M Sc thesis, St. Xavier’s College (Autonomous), Tirunelveli, Tamil Nadu,

p 59.

4.3 Suganya K R. 2010. ‘Digestive enzyme activities of Channa striatus fingerlings fed with

selected medicinal herbs.’ M Sc thesis, Nandha Arts and Science College, Erode, Tamil

Nadu, p 29.

4.4 Tamil Elakkiya B. 2010. ‘Digestive enzyme activities of Channa marulius fingerlings fed

with selected probiotics.’ M Sc thesis, Nandha Arts and Science College, Erode, Tamil Nadu,

p 32.

4.5 Jafar Sathik S. 2010. ‘Intraspecific hybridization between two different populations and

RAPD analysis of Murrel Channa striatus’. M Sc thesis, Jamal Mohamed College,

Thiruchirapalli, Tamil Nadu, p 43.

4.6 Vigneshwaran R. 2011. ‘Effect of dietary vitamins on growth and protein variability of

Channa striatus fingerlings.’ M Sc thesis, Jamal Mohamed College (Autonomous),

Thiruchirapalli, Tamil Nadu, p 62.

4.7 Ashfaq Ahmed S. 2011. ‘Studies on pathogenicity and treatment of Channa marulius

infected with Aeromonas hydrophila.’ M Sc thesis, Jamal Mohamed College (Autonomous),

Thiruchirapalli, Tamil Nadu, p 44.

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4.8 Riyaz Ahmed A. 2011. ‘Molecular identification and diagnostics of Epizootic Ulcerative

Syndrome (EUS) in Striped Murrel Channa striatus.’ M Sc thesis, Jamal Mohamed College

(Autonomous), Thiruchirapalli, Tamil Nadu, p 34.

4.9 Chandrasekar B. 2012. ‘Effect of Clove oil on Hematology of Channa striatus challenged

with Aeromonos hydrophila.’ M Sc thesis, Jamal Mohamed College (Autonomous),

Thiruchirapalli, Tamil Nadu, p 40.

4.10 Imthathulla S. 2012. ‘A comparative study of Antioxidant enzymes and lipid peroxidation

in selected murrel species challenged with Aeromonas hydrophila.’ M Sc thesis, Jamal

Mohamed College (Autonomous), Thiruchirapalli, Tamil Nadu, p 26.

PhD Theses:

4.11 Dhanaraj M. 2009. ‘Probiotics as immunostimulants in striped murrel Channa striatus.’

Ph D thesis, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, p 127.

4.12 Mehraj Ud Din War. 2012. ‘Aspects of development and larval rearing of snakehead

murrel Channa striatus (Bloch 1793) using different feed.’ Ph D thesis, Madras University,

Chennai, Tamil Nadu, p 108

4.13 Bilal Ahmad Paray. 2012. ‘Histological studies on ontogenic development and larval

rearing of striped murrel Channa striatus.’ Ph D thesis, Manonmaniam Sundaranar

University, Tirunelveli, Tamil Nadu, p 165.

5. Popular Article:

5.1 Haniffa M A. 2009. ICAR NAIP sponsored workshop on breeding and seed production of

murrels. Fishing Chimes 29(8): 48 -51.

5.2 Haniffa M A, Dhanaraj M, Muthu Ramakrishnan C, James Milton M, Arunsingh S V and

Ajaz Ali. 2009. Backyard Murrel Culture for Women. Infofish International 6:14 -16.

5.3 Haniffa M A, Dhanaraj M, Muthu Ramakrishnan C, Arunsingh V and Manikandaraja D.

2010. Fecundity of C. striatus (Bloch 1793) collected from Tambraparni River. Fishing

Chimes 30(7): 7-8.

5.4 Haniffa M A, Bilal Ahmad Paray and Manikandaraja D. 2010. Trainings on Seed production,

feed formulation, and culture of murrels. Fishing Chimes 30(8): 35-38.

5.5 Haniffa M A, Bilal Ahmad Paray and Manikandaraja D. 2010. ICAR-NAIP sub-project

workshops on seed production, feed formulation and disease management in murrel culture.

Fishing Chimes 30(8): 39-42

5.6 Haniffa M A, Seenappa D, Manikandaraja D and James Milton M. 2011. Murrel Farming

training to Fish farmers of Karnataka. Fishing Chimes 30(10&11): 52-53.

5.7 Kumar K, Eknath A E, Sahu A K, Mohanty U L, Kumar R, Sahoo M and Jahan N. 2011.

Snake heads: Challenging fish for diversification of fish farming. Fishing Chimes 31(1): 110-

113.

5.8 Sahu B B, Kumar K, Sahu A K, Mohanty U L, Kumar R, Sahoo M, Jahan N and Eknath A E,

2011. Processing and value addition to murrel in value chain. Fishing Chimes 31(1): 106-

108.

5.9 Haniffa M A. 2011. Popularization of Murrel Farming among Fish Farmers of Hyderabad.

Fishing Chimes 31(2): 27-29

5.10 Haniffa M A. 2011. A value chain on murrel production for income generation. Fishing

Chimes 31(5): 19-21

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5.11 Haniffa M A. 2012. ICAR- NAIP Sponsored One day Training Programme on – Seed

Production, Feed Formulation and Culture of Murrels: 22 Sept 2011. Fishing Chimes 31(12):

18-20

5.12 Haniffa M A and Mohamed Riyaz H. 2012. Murrel Fish Food Expo 2012. Fishing

Chimes 32(5): 9-12

5.13 Haniffa M A, Jeyasheela P, Manikandaraja D, Jafar Sathik S, and Bilal Ahmad. 2013.

Nellai Fish Food Festival 2013. Fishing Chimes 33(6): 18-24

5.14 Haniffa M A, Jeyasheela P, Manikandaraja D, Jafar Sathik S, and Bilal Ahmad. 2013.

Nanjil Food Fish Festival 2013. Fishing Chimes 33(6): 25-28.

6. Newspaper Article:

6.1 Haniffa M A. 2008. Rs 3 crore grant to train fish farmers on Murrel culture. The Hindu,

14.10.2008.

6.2 Haniffa M A. 2008. Viral meen urpathi payirchi. Dinamalar, 14.10.2008.

6.3 Haniffa M A. 2008. Arithagi varum viral meen urupathiyai peruka mathiya arasu Rs 3.2

crore Nithi uthavi. Dinakaran, 14.10.2008.

6.4 Haniffa M A. 2008. Viral meen aaraichikaga Rs.3 crore othukeedu. Daily Thanthi,

15.10.2008.

6.5 Haniffa M A. 2008. The unemployed can easily earn Rs.30,000/- to Rs 40,000/- a year from

a small pond. The Hindu, 19.10.2008.

6.6 Haniffa M A. 2009. Murrel fish training to farmers. The Hindu, 14.03.2009.

6.7 Haniffa M A. 2009. Great demand for Murrel fish. The Hindu, 17.03.2009.

6.8 Haniffa M A. 2009. Viral meen payirchi thuvangiathu. Dinamalar, 17.03.2009.

6.9 Haniffa M A. 2009. CARE aims st socio economic upliftment through Murrel farming. The

New Indian Express, 11.06.2009.

6.10 Haniffa M A. 2009. Vivasaigaluku meen valarpu payirchi 30th

June Nadakirathu. The

Daily Thanthi, 11.06.2009.

6.11 Haniffa M A. 2009. 200 ton viral meengal urupathi seya plan. Dinakaran, 1.07.2009.

6.12 Haniffa M A. 2009. Viral meen kunjugal urupathiku athiga vivasaiyigal thaevai.

Dinamalar, 1.07.2009.

6.13 Haniffa M A. 2010. Good scope for Murrel culture. The new Indian Express, 28.1.2010.

6.14 Haniffa M A. 2010. Viral meen valarkamunvarum magalir kuluvinarku uthavigal.

Dinakaran , 3.02.2010.

6.15 Haniffa M A. 2010. Farmers to learn new fish culture Techniques. The Hindu, 3.02.2010.

6.16 Haniffa M A. 2010. Viral meen kunju valarpathu kurithapayirchi mugaam. Daily

Thanthi, 3.02.2010.

6.17 Haniffa M A. 2010. Viral meen kuritha aaraichigal, vivasaikalai sendradayavendum.

Dinamalar, 23.3.2010.

6.18 Haniffa M A. 2010. Neervalam konda kulangalil viral meen valarga munvaravendum.

Daily Thanthi, 25.03.2010.

6.19 Haniffa M A. 2010. Viral meengalai athigalavu urupathi seya vendum. Dinamalar,

25.3.2010.

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6.20 Haniffa M A. 2010. Valedictory function of training programme on Murrel farming held.

The New Indian Express, 25.03.2010.

6.21 Haniffa M A. 2010. Mana manakum viral Piriyani. Daily Thanthi, 28.03.2010.

6.22 Haniffa M A. 2010. Viral meen valarpil athiga labam peralam. Dinamalar, 7.7.2010.

6.23 Haniffa M A. 2010. From a poor driver to successful fish farmer. The Hindu, 15.12.2010.

6.24 Haniffa M A. 2010. Viral meen valarpu payirchi mugaam. Daily Thanthi, 15.12.2010.

6.25 Haniffa M A. 2010. Viral meen kunju urupathi mulam varudathirku Rs. 15 lakh

varumanam peralam. Dinamalar, 15.12.2010.

6.26 Haniffa M A. 2011. Viral meen il irunthu Karpinigaluku marunthu. Daily Thanthi,

12.08.2011.

6.27 Haniffa M A. 2011. Viral meen eatrumathiku nalla chanthai vaippu. Dinamani,

12.08.2011.

6.28 Haniffa M A. 2011. Fish farmers Training on seed production and culture of Murrels.

Daily Thanthi, 23.11.2011.

6.29 Haniffa M A. 2011. Viral meenin magathuvamum maruthuvamum. Daily Thanthi,

18.12.2011.

6.30 Haniffa M A. 2012. Viral meen unavu kankatchi. Daily Thanthi, 8.1.2012.

6.31 Haniffa M A. 2012. Murrel fish food mela today. The Hindu, 9.1.2012.

6.32 Haniffa M A. 2013. Meen unavin thaevai nalukunal athigaripu, Dinamani, 8.02.2013.

6.33 Haniffa M A. 2013. Palai il unavu Thiruvila. Dinakaran, 9.02.2013.

6.34 Haniffa M A. 2013. Palai il viral meen unavu kankatchi. Dinamani, 31.07.2013.

6.35 Haniffa M A. 2013. Training for inland fish farmers in Murrel culture. The Hindu,

1.08.2013.

6.36 Haniffa M A. 2013. Viral meen idly podi. Dinakaran, 1.08.2013.

6.37 Haniffa M A. 2013. Viral valarpu karutharangu. Daily Thanthi, 1.08.2013.

6.38 Haniffa M A. 2013. Viral valarpu and unavu kankatchi. Dina malar, 1.08.2013.

6.39 Haniffa M A. 2013. Viral meen valarpathu eppadi. Daily Thanthi, 13.09.2013.

6.40 Haniffa M A. 2013. Viral meen valarkalam. Daily Thanthi, 12.12.2013

7. Seminar/ Symposium/Conference/Workshop Proceedings

7.1 Haniffa M A. 2010. Effect of Effinol ® FG incorporated diets on growth response of Striped

Snakehead Channa striatus (in) Proceedings of International Conference on World

Aquaculture 2010 - General Aquaculture session held during 1 – 5 March 2010 at San Diego,

California.

7.2 Haniffa M A. 2011. Effect of Probiotics on growth and microbiological changes in snakehead

Channa striatus challenged by Aeromonas hydrophila (in) Proceedings of International

Conference on Aquaculture America 2011 - Fin Fish Nutrition session held during 28th

February – 3rd

March 2011 at New Orleans Marriott, New Orleans, Louisiana.

7.3 Haniffa M A and Abdul Kader Mydeen K P. 2012 Effects of medicinal herbs on

haematological and biochemical changes in Channa punctatus, Heteropneustes fossilis and

Cyprinus carpio challenged by Aeromonas hydrophila (in) Proceedings of International

Conference on AQUA 2012, held during 1 – 5 September 2012 at Prague Czech Republic.

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7.4 Haniffa M A and Abdul Kader Mydeen.K P. Isolation and identification of microflora Of

Diseased Channa punctatus, Heteropneustes fossilis and Cyprinus carpio and Treatment By

Medicinal Herbs (in) Proceedings of International Conference AQUA 2012, held during 1 –

5 September 2012 at Prague Czech Republic.

7.5 Kumar K, Damle, D K, Jahan N, Sahoo M, Mohanty U L, Kumar R. 2010. Formulation of

cost effective feeds from locally available animal based ingredients for Channa striatus

fingerlings (in) Golden Jubilee National Seminar on Diversification of Aquaculture through

locally available fish species (DALAF-2010) held on 27-28 August 2010 at CIFE Centre,

Kolkata, India

7.6 Kumar K, Mohanty U L, Kumar R, Jahan N, Damle, D K and Sahoo M. 2011. Production of

snakeheads with different stocking densities in pond ecosystem. (in) Proceedings of

International Conference on Aquaculture Asia held during 17-21 January 2011 at Kochi,

India.

7.7 Mohanty U L, Kumar R, Jahan N and Sahoo M. 2011. Effect of medicinal plant crude

extracts on the health of Channa striatus fingerlings (in) Proceedings of National Conference

on 9th

Indian Fisheries Forum held during 19-23 December 2011 by CMFRI at Chennai,

India.

7.8 Kumar K, Sahoo M, Jahan N, Sahu A K, Kumar R, Mohanty U L and Eknath A E. 2011.

Murrel Culture-A panacea for the Farmers (in) Proceedings of National Conference on 13th

Indian Agricultural Scientists and Farmers’ Congress held during 19-20 February 2011 at

Allahabad, India

7.9 Kumar R, Mohanty U L, Kumar K, Sahoo M, Sahu A K, Jena J K and Jayasankar, P. 2012.

Murrel Farming: A New initiative in Odisha (in) Proceedings of International Conference on

Global Symposium on Aquatic resources for eradicating hunger and malnutrition-

opportunities and challenges held during 4-6 December 2012 at Mangalore Fisheries

College, Mangalore, India

7.10 Kumar R. 2012. Possibilities of murrel culture in Northern India (in) Proceedings of

International Conference on 14th

Indian Agricultural Scientist and Farmers’ Congress on

Agriculture and Agripreneurship organized by Bioved research society, held during 18-19

February 2012 at Allahabad, U.P.

7.11 Haniffa M.A. 2012. Problems and Challenges of Indian Aquaculture and Attempts by

CARE- A Review (in) Proceedings of National Conference on Indian Science Congress

Association, Regional Science Congress meet, Coimbatore Chapter, held during 15 & 16th

December 2012, Kongunadu Arts and Science College, Coimbatore, Tamil Nadu, India.

7.12 Haniffa M A. 2013. Biodiversity and Food security (in) Proceedings of National

Conference on Food security Issues and Concerns, held during 21- 22 February, 2013,

Arulanandar College, Karumathur, Tamil Nadu, India.

8. Technical Bulletin:

8.1 Kumar K, Sahu A K, Kumar R, Mohanty U L, Sahoo M, Jahan N, Jena, J K and Eknath A E.

2010. Farming of Snakeheads. CIFA, Bhuabneswar.

8.2 Kumar K, Sahu B B, Kumar R, Mohanty U L, Sahoo M, Jahan N, Sahu A K, Jena, J K and

Eknath A E. 2010. Processing and value addition of murrel. CIFA, Bhuabneswar.

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9. Manual

9.1 Haniffa M A, Dhanaraj M, Muthu Ramakrishnan C, Arunsingh V and Manikandaraja D.

2009. Veral Meen Valarpu (Tamil) Murrel Fish Culture Centre for Aquaculture Research and

Extension (CARE), St.Xavier’s College, Palayamkottai, Tamil Nadu, India, p 20

9.2 Kumar K, Kumar R, Mohanty U L, Jahan N, Sahoo M and Sahu A K 2010. (Odia) (Murrel

Seed Production and Culture) Central Institute of Freshwater Aquaculture, Bhubaneswar,

India, p20

9.3 Kumar K, Sahu B B, Kumar R, Mohanty U L, Jahan N, Sahoo M and Sahu A K 2010. Value

Addition: Flavours from Murrel (bilingual) Central Institute of Freshwater Aquaculture,

Bhubaneswar. p32

9.4 Kumar K, Kumar R, Mohanty U L, Sahoo M and Sahu A K. 2011. Murrel farming- A manual

for farmers. Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar,

India, p13

9.5 Kumar K, Kumar R, Mohanty U L, Sahoo M, Mohanty A K and Sahu A K. 2012. Murrel

Culture and its popularization. Central Institute of Freshwater Aquaculture, Kausalyaganga,

Bhubaneswar, India, p25.

9.6 Kumar R, Mohanty U L, Sahoo M, Sahu A D and Sahu A K. 2013. Murrel Machha chasa,

prajanana o janla utpadana (Odia) (Breeding, Seed production and culture of murrel). Central

Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, India, p25.

10. Seminar/ Symposium/Conference/Workshop Presentation

10.1 Manikandaraja D. 2011. Morphometris and meristic development of larval and juvenile

snakehead Channa stratus (Bloch 1793). (in) Asian Pacific Aquaculture, held during 17-20

January 2011 at Kochi, Kerala.

10.2 Bilal Ahmed Paray. 2011. Induced breeding and seed production of striped murrel

Channa striatus. (in) Asian Pacific Aquaculture, held during 17-20 January 2011 at Kochi,

Kerala.

10.3 Hameedullah Sherief S, Haniffa M A, Kavitha K and Riyaz Ahmed A. 2012. Molecular

identification of Epizootic Ulcerative Syndrome (EUS) in striped murrel Channa striatus (in)

National Conference on Biotechnological Approaches in Aquaculture, Live Aqua 2012, held

during 1-3 February 2012 at School of life Sciences, Bharathiyar University, Coimbatore,

Tamil Nadu.

10.4 Kavitha K, Haniffa M A and Sunitha K S. Prophylaxis and treatment of Epizootic

Ulcrerative Syndrome (EUS) in murrels (in) National Conference on Biotechnological

Approaches in Aquaculture, Live Aqua 2012, held during 1-3 February 2012 at School of life

Sciences, Bharathiyar University, Coimbatore, Tamil Nadu.

10.5 Manikandaraja D, Haniffa M A and Shanthi P. Length – Weight Relationship and

Condition Factor of Captured and Cultured Snakehead Channa striatus(in) National

Conference on Biotechnological Approaches in Aquaculture, Live Aqua 2012, held during 1-

3 February 2012 at School of life Sciences, Bharathiyar University, Coimbatore, Tamil Nadu.

10.6 Bilal Ahmad Paray and Haniffa M.A. Effect of certain feeds on growth and survival

of striped snakehead, Channa striatus (Bloch, 1793) hatchlings as test starter diets under

captive conditions (in) Indian Science Congress Association, Regional Science Congress

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meet, Coimbatore Chapter, held during 15 & 16th

December, 2012 at Kongunadu Arts and

Science College.

10.7 Jeya Sheela P, Haniffa M.A and Kavitha K. Medicative Values of Striped Murrel,

Channa striatus (in) Indian Science Congress Association, Regional Science Congress meet,

Coimbatore Chapter, held during 15 & 16th

December, 2012 at Kongunadu Arts and Science

College.

11. CDs/Videos:

11.1 Haniffa M A. 2010. ICAR- NAIP Sub project activities – 2 minute Video Coverage (2009

-2010). Centre for Aquaculture Research and Extension (CARE), St.Xavier’s College

(Autonomous), Tamil Nadu

11.2 Haniffa M A. 2010. Murrel Culture Training Programme - Hyderabad, Chennai and

Coimbatore. Centre for Aquaculture Research and Extension (CARE), St.Xavier’s College

(Autonomous), Tamil Nadu

11.3 Haniffa M A. 2010. Murrel Seed Production at CARE Aquafarm. Centre for Aquaculture

Research and Extension (CARE), St.Xavier’s College (Autonomous), Tamil Nadu

11.4 Haniffa M A. 2010. Murrel Seed Production at Vallanadu Fish Farm. Centre for

Aquaculture Research and Extension (CARE), St.Xavier’s College (Autonomous), Tamil

Nadu

11.5 Haniffa M A. 2010. Pelleted Feed Preparation for Large and Small Scale Murrel Culture.

Centre for Aquaculture Research and Extension (CARE), St.Xavier’s College (Autonomous),

Tamil Nadu

11.6 Haniffa M A. 2010. Murrel Feeding at Vallanadu Fish Farm. Centre for Aquaculture

Research and Extension (CARE), St.Xavier’s College (Autonomous), Tamil Nadu

11.7 Haniffa M A. 2010. Murrel Growout Culture at High Ground Aquafarm – Tirunelveli.

Centre for Aquaculture Research and Extension (CARE), St.Xavier’s College (Autonomous),

Tamil Nadu

11.8 Haniffa M A. 2011. ICAR- NAIP Sub project activities – 2 minute Video coverage (2010

- 2011). Centre for Aquaculture Research and Extension (CARE), St.Xavier’s College

(Autonomous), Tamil Nadu

11.9 Haniffa M A. 2011. Murrel Seed Production at Araikulam Fish Farm. Centre for

Aquaculture Research and Extension (CARE), St.Xavier’s College (Autonomous), Tamil

Nadu

11.10 Haniffa M A. 2011. Murrel Parental Care at Araikulam Fish Farm. Centre for

Aquaculture Research and Extension (CARE), St.Xavier’s College (Autonomous), Tamil

Nadu

11.11 Haniffa M A. 2011. Semi Moist Feed Preparation for Murrel Culture. Centre for

Aquaculture Research and Extension (CARE), St.Xavier’s College (Autonomous), Tamil

Nadu

11.12 Haniffa M A. 2011. Murrel Table – Size Fish Production at CARE. Centre for

Aquaculture Research and Extension (CARE), St.Xavier’s College (Autonomous), Tamil

Nadu

11.13 Haniffa M A. 2011. Murrel Growout Culture at Arasan Aquafarm – Araikulam. Centre

for Aquaculture Research and Extension (CARE), St.Xavier’s College (Autonomous), Tamil

Nadu

11.14 Haniffa M A. 2011. Murrel Harvest at Araikulam Fish Farm. Centre for Aquaculture

Research and Extension (CARE), St.Xavier’s College (Autonomous), Tamil Nadu

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11.15 Haniffa M A. 2011. Murrel Harvest at Kaliyavoor Fish Farm. Centre for Aquaculture

Research and Extension (CARE), St.Xavier’s College (Autonomous), Tamil Nadu

11.16 Haniffa M A. 2011. Murrel Fillet Preparation. Centre for Aquaculture Research and

Extension (CARE), St.Xavier’s College (Autonomous), Tamil Nadu

11.17 Haniffa M A. 2011. Murrel Value Addition (Murrel Briyani, Cutlet, Fingerchips, Pickle).

Centre for Aquaculture Research and Extension (CARE), St.Xavier’s College (Autonomous),

Tamil Nadu

11.18 Haniffa M A. 2012. Murrel Harvest at Araikulam Fish Farm. Centre for Aquaculture

Research and Extension (CARE), St.Xavier’s College (Autonomous), Tamil Nadu

11.19 Haniffa M A. 2012. Murrel Harvest at Veeralaperunselvi Fish Farm. Centre for

Aquaculture Research and Extension (CARE), St.Xavier’s College (Autonomous), Tamil

Nadu

11.20 Haniffa M A. 2012. Murrel Fish Food Expo 2012. Centre for Aquaculture Research and

Extension (CARE), St.Xavier’s College (Autonomous), Tamil Nadu

11.21 Haniffa M A. 2013. Murrel Fish Cookies. Centre for Aquaculture Research and Extension

(CARE), St.Xavier’s College (Autonomous), Tamil Nadu

11.22 Haniffa M A. 2013. Mass Culture of Live Feed. Centre for Aquaculture Research and

Extension (CARE), St.Xavier’s College (Autonomous), Tamil Nadu

11.23 Haniffa M A. 2013. Nellai Fish Food Festival 2013. Centre for Aquaculture Research and

Extension (CARE), St.Xavier’s College (Autonomous), Tamil Nadu

11.24 Haniffa M A. 2013. Nanjil Fish Food Festival 2013. Centre for Aquaculture Research and

Extension (CARE), St.Xavier’s College (Autonomous), Tamil Nadu

11.25 Sahu B B, Kumar K, Kumar R, Mohanty U L, Sahu A K, Sahoo M, Jahan N and Eknath

A E, 2010. Value addition of murrel. Central Institute of Freshwater Aquaculture, Orissa.

12. Popular article in other Language

12.1 Haniffa M A. 2010. Viru Viru Labam Tharum Viral Valarppu (Tamil) (Murrel Fish

Culture for Fast Income). Pasumai vikadan, 10.11.2010, p 4-9

12.2 Haniffa M A. 2011. Villangam Illatha Viral Valarppu (Tamil) (Harmless Murrel Fish

Culture). Pasumai vikadan, 25.09.2011, p 40-41

12.3 Haniffa M A. 2011. Viral Meen Valarppu Payirchi Mugaam (Tamil) (Murrel Fish

Training). Valarum Thamilagam, November 2011, p 34-35.

12.4 Haniffa M A. 2011. Pambuthalai Viralmeen, Meen Valarporukku Oru Varaprasatham

(Tamil) (Snakehead murrel fish culture- a boon to fish farmers). Valarum Thamilagam,

December 2011, p 36-37

12.5 Haniffa M A. 2011. Veeru Nadai Podum Viral Valarpu (Tamil) (Fast Growing Murrel

Culture). Pasumai vikadan, 10.12.2011, p 28-31.

12.6 Kumar K, Kumar R, Mohanty U L and Sahu A K. 2012. Murrel Maccha Chasa O tara

Prasara (Odia) (Murrel farming and its popularization). Meenalok, FISHCOPFED, Govt. of

India, October-December 2012, p3-6.

12.7 Haniffa M A. 2012. Thennaiku Idail Viral (Tamil) (Murrel Culture amidst Coconut

Trees). Pasumai vikadan, 10.02.2012, p 29-31

12.8 Haniffa M A. 2013. Mana Aluthama, Viral Meen Biscuit Sapidunga (Tamil) (Murrel

biscuits a cure for depression). Kungumam, 26.08.2013, p 95-96.

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12.9 Kumar K, Kumar R, Mohanty U L and Sahu A K. 2013. Sal seula macchara janla

utpadan (Odia) (Seed production of murrel) Meenalok FISHCOPFED, Govt. of India, January-

March 2013, p 10-13.

13. Folder/Leaflet/Handout:

14. Report:

……………………………………………………………………………………………………

Note:

Name of CPIs and CCPIs to be given in italics

Journal name to be given in full

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Annexure II

Murrel Value Added Products

Responsible Partner: CARE, St.Xavier’s College, Tamil Nadu, Lead Institute

a) Ready To Serve Products:

1. Murrel fry:

Clean Murrel fillet of ½ Kg and cut into small pieces. Prepare a masala paste by mixing

lemon juice (2 tsp), pepper powder (5 g), cumin seed powder (5 g), rice flour (10 g), salt (to

taste), and water. Marinate the fish pieces in the masala paste for an hour and shallow fry in oil

and serve hot.

2. Murrel gravy:

Clean Murrel fillet of ¼ Kg and cut into small pieces. Prepare a masala paste by grinding

red chillies (8 nos), cumin seeds (2 tsp) and small onion (10 Nos) and mix with turmeric powder

(½ tsp), tamarind pulp (1 lemon size), salt (to taste) and sesame oil (2 tbsp). Boil the masala for

10 minutes and add the fish pieces to the gravy. Cook until oil separation and serve hot.

3. Murrel Briyani:

Soak Basmati rice (1 Kg) in water for half an hour. Clean Murrel fillet of 1 Kg, pat dry,

and cut into 1 inch pieces. Smear the fish pieces with spice paste of ginger – garlic paste (4 tsp),

chilli powder (2 tsp) and salt to taste and marinate for half an hour. Deep fry the marinated fishes

and keep aside. In a pressure cooker, add spices and allow to splutter. Sauté chopped onion (1

Kg) in ghee till golden brown colour. Stir fry chopped tomatoes (½ Kg) and reduce to pulp till oil

separation. Then add a cup of curd, chilli powder (2 tsp), garam masala powder (1 tsp), turmeric

powder (1 tsp), lemon juice (1 No.) and salt (to taste) and sprinkle with finely chopped coriander

and mint leaves. Add double the quantity of water as that of rice, allow boiling and pressuring

cook for 10 minutes. Add fried fish and ghee; stir well and serve hot.

4. Murrel Vermicelli:

Fry finely chopped big onions (75 g), green chillies (10 g), coriander leaves (10 g) and

garlic (25 g) in oil. To the above, add 400 ml of water, salt (to taste) and turmeric powder (a

pinch) and allow to boil. Then add fish pieces (200 g), toasted vermicelli (200 g) and cook for

5 minutes and served hot.

5. Murrel Pollichathu:

Sauté finely chopped ginger and garlic (30 g each) in oil; Toss chopped onions (¾ Kg)

and allow to cook. Heat half tablespoon (tbsp) of oil. To this add chilly (¾ tsp) and turmeric

powder (1 tsp) mixed with a little water to form a ball (so that the powders don’t burn while

sautering) and sauté for some time. Add the chopped tomatoes (¼ Kg) and sauté till done.

Remove from fire and keep aside

To make the Packets: Heat the banana leaves gently over fire to make it pliable. Smear

the inside of the leaves with a drop of oil. Put some sautéed mixture in the centre of the leaf.

Place a piece of fish and some mixture to cover the fish pieces. Fold over and make a parcel, and

tie with banana strings or secure it with a toothpick. Heat a thick-bottomed pan. Pour

2 tbsp oil and heat. Arrange the packets and cover the pan with a lid. Put live coal on top and fire

below. (If looking on gas, you can do away with the coal on top). Turn the fish over when one

side of the banana leaf turns pale brown in colour. Cook till the other side also turns pale brown.

Remove from fire and serve hot

6. Murrel Cutlet:

Wash the fish fillets (½ Kg) and smear with chilli powder (½ tsp), turmeric powder

(1/8 tsp) and salt and keep aside for half an hour. Deep fry the marinated fish and keep aside. Pour

oil in a pan, as the oil becomes hot, add finely chopped big onion (No. 1), ginger, garlic and

green chillies and half fry. Stir continuously. Now add fried fish to it and mix well (Do not add

water). Cook till it dry. Now add pepper powder (1 tsp), salt, and garam masala (2 tsp). Fry well.

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Add smashed potatoes (½ Kg) and mix well. Turn off the gas and spread some coriander leaves.

Let it cool for some time; then make round balls and make patties. Take a bowl, beat the eggs

and in another plate, take bread crumbs. Dip the balls in the egg mixture and then put bread

crumbs on both sides of the cutlet. Now deep fry the cutlets in oil and serve hot with tomato

ketchup.

7. Murrel Pickle:

Marinate the cleaned fish pieces (½ Kg) with turmeric powder (½ tsp), chilli powder (4

tsp), and salt and keep aside for ½ an hour. Heat the gingly oil, and fry the fish pieces till they

become brown. Remove the fish pieces from oil and keep aside. Saute the chopped ginger (1

tsp), garlic (1 tsp), Fenugreek (½ tsp) and green chilli (4 nos). Remove it from the oil and add

fried fish pieces. Mix chili powder with vinegar and pour the mixture in the pan, add desired

amount of salt, sugar and wait for 5 minutes. Now add fried fish pieces and allow it to boil for 15

minutes. Allow it to cool and fill in clean sterile bottles.

8. Murrel Soup:

Murrels are slimy with mucus all over the body. Kill the fish (2 nos) by hitting on the

head. Cut off the fins and scrap the scales. Cut into somewhat thin round pieces. Wash the pieces

thoroughly by rubbing the sides against some rough surface using salt and water until they are

mucus free. Soak tamarind (100 g) in warm water, extract the juice and set aside. Peel the

mangoes (2 nos) and slice into thin pieces. Apply turmeric powder (1 tsp), red chilly powder (4

tsp), ginger-garlic paste (2 tsp) and two spoons of oil to the fish pieces and marinate for thirty

minutes. Grind the garlic (12 nos), cloves (6 nos), cardamoms (6 nos), one cup of onions,

mustard seeds (2 tsp) and adding three spoons of oil, into a smooth paste. Add oil to a deep

bottomed pan and heat. Splutter remaining mustard seeds. Add remaining onion pieces, green

chillies and the curry leaves and fry. Now add the marinated fish pieces, mango pieces and the

ground paste. Fry for about ten minutes turning the contents up and down taking utmost care to

see that the fish pieces are not broken. Now add the tamarind juice and enough salt. Allow to

simmer until the dish is done. Serve hot with rice varieties.

9. Murrel Curry:

Heat the oil and fry the Fenugreek, mustard and garlic paste. Add the chopped onion and

fry to rich golden brown. Then add green chillies (8 nos), tomato (75 g), tamarind pulp (50 g),

turmeric powder (1 tsp), coriander powder (25 g), chilli powder (25 g), salt, pepper powder (1

tsp), cumin seed powder (1 tsp) to the above and mix well and cook to get gravy. To the gravy

add the fish (¼ Kg) and keep in flame until the fish is cooked. Finally add the powdered jaggery,

stir well and remove from fire and serve hot.

10. Murrel Poori: For stuffing: Mix cleaned and chopped fish fillet (100g) along with the salt, whisked egg

(No. 1), sesame oil (3 ml), chopped spring onions (20 g) and green chillies (2 nos). Divide the

fish mixture into 10 equal portions.

For covering: Shift wheat flour (3 cups) and salt into a medium bowl. Add 1tsp oil and

mix in enough of the water to make soft dough. Knead dough for few minutes on a lightly oiled

surface, with a little oil rubbed into hands to prevent them from sticking, until dough is soft and

pliable. Cover with plastic wrap and let rest it for 10 minutes. Divide dough into 10 pieces; roll

into balls. Dust balls with flour and cover with a damp cloth. Flatten it with hand and add a small

ball of the stuffing inside and close it completely and try to spread it out with hand with moisten

it with oil and fold to seal. Repeat the same process with the remaining fish mixture and balls.

Heat the oil in a pan and deep fry the poori. Remove, drain the excess oil and serve hot.

11. Murrel Finger chips:

Wash the fish fillet (¼ Kg) thoroughly and slice the fillet into long finger like pieces.

Apply salt, pepper powder (2 tsp) and lemon juice (1 no.); keep aside for 1 hour. Beat the eggs

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(3 nos). Heat oil in a frying pan and dip the fish piece in egg white and roll it in the bread

crumbs. Deep fry in hot oil and serve hot.

12. Murrel Fish Puttu:

Clean and steam cook the murrel fillet (½ Kg). Chop onion (10 nos), ginger (20 g), garlic

(4 flakes), green chillies (5 nos) finely. Heat oil in a pan and sauté mustard (1 tsp), curry leaves

and all chopped ingredients till golden brown. Now add the cooked fillet, mix evenly and add

salt to taste. The dish is now ready to serve.

13. Murrel Fish Moilee: Slice the big onion (1 no.) nicely and paste the ginger (5 g) nicely. Take the coconut milk

from half Coconut. Chop the Tomato (2 no.) & Green Chillies (12 nos). Mix the Salt with Fish

(½ Kg) and rest it for 15 minutes. Semi Fry the fish in hot oil. Heat the oil; fry the onion, Ginger,

green chillies, curry leaves, tomato, till golden brown. Add the Coconut Milk, Fish, Salt and

cook for 10 minutes. Then add the corn flour (½ tsp) and lemon juice (1 no.) and boil for a while

and serve hot.

14. Murrel Fish Chops: Clean the fillet (¼ Kg) and cut into small pieces. Chop the onion (100 g), ginger (1 inch),

garlic (6 flakes), red chilli (4 nos) finely. Heat oil in a pan and fry the chopped onion, ginger,

garlic, red chilli, coriander (1 tsp), and pepper (2 tsp) until it is done and allow it to cool. Grind

the above ingredients in a mixer. Pour oil in a pan and sauté mustard seeds and curry leaves.

Then add the cleaned fillet pieces, ground mixture and add little water. Allow it to cook in low

flame for 15 minutes. The dish is now ready to serve with rice.

15. Murrel Fish Soup: Clean and break the murrel fish bones (½ kg) into small pieces. Boil it with water (12

soup cups), finely sliced onions (100g), clove (6 nos.), cinnamon (2 pieces), pepper corns (5

nos), and salt (1 tbsp), till the water is reduced to 10 cups. Strain the soup and keep aside. Saute

the onion (200 g) in ghee (2 tbsp) till golden brown in colour in a vessel big enough to hold the

stock. Add the flour and sauté till flour is done. Add coarsely powdered pepper (2 tbsp) & salt.

Pour in the stock & boil for few minutes on low flame. Remove from fire. Heat the ghee / butter

& fry the onions (25 g) till golden brown. Add this to the soup just before serving.

16. Murrel Fish Tandoori:

Clean the fish fillet (1 kg) & cut them into large pieces. Paste the small onion (2 nos),

brinji leaves (1 no.), chinnamon (1 no.), ginger (15 g), garlic (4 flakes), cardamom (3 nos),

cashew nut (1 tsp), red chillies (6 nos) nicely. Mix the salt, red colour powder (1 tsp), lemon

juice (1 no.), curd (1 cup), ground masala & fish together well. Rest it for 2 hours & start frying

in the mud stove (or) baking oven. Add oil simultaneously and cook well as roasted. Serve Hot.

17. Murrel Fish Balls:

Cut big onion (200 g) into small pieces & fry till golden brown. Add chilli powder (30 g),

coriander powder (10 g) & turmeric powder (1 tsp) to the fried onion. Mix minced fish (1 Kg)

with the fried onion and masala mixtures. Add beaten eggs (1 no.), bread powder (30 g) & salt.

Mix homogenously. Prepare into small balls of about 3 cm diameter. Steam for 10-15 min. The

balls can also be fried and served.

18. Murrel Fish Bajji:

Marinate the cleaned fish pieces (½ kg) with maida (200 g), soya four (150 g), chilli

powder (1 tbsp), lemon juice (2 tsp) and salt; keep aside for half an hour. Heat the oil, and fry

the fish pieces till it becomes brown. Remove the fish pieces from oil and serve with tomato

sauce.

19. Murrel Fish Bonda:

Grind the minced fish (½ kg) with salt, maida (100 g), butter (50 g) and spices – pepper

powder (3 tsp), chopped green chilli (4 nos) and coriander leaves (a fistful). Mould it in metallic

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or wooden moulds of different shapes. The thickness must not exceed 1cm. Cool the moulded

fish cakes in a freezer till it becomes semi-frozen. Make the batter by mixing the maida with

chilled water in the ration of 1:1. Prepare the breading mixture (150 g) by powdering dried bread.

Dip the semifrozen fish cakes in the batter and spread the bread powder uniformly over it. Fry

the frozen fish bonda in hot oil till it attains a golden yellow colour.

20. Murrel Cookies:

The first patent in Murrel Cookie is an edible food product prepared from Murrel fillet

and it is rich in protein and Polyunsaturated fatty acids (Per serving of Murrel cookies contain:

Protein: 0.65 g; Calories: 53; Fat: 3.47g; Carbohydrates: 7.34g) has been filed on 30.04.2013

with the assistance of Metayage IP Strategy Consulting Ltd, Coimbatore.

21. Murrel Millet Mix: Ready to eat products viz: Murrel Wheat Kakhara, Murrel Ragi Kakhara, Murrel Rice

Kakhara, Murrel Pasta and Murrel Idli and Dosa have been prepared due to their richness in

Omega 3 and 6 fatty acids to overcome malnutrition. A combination of Murrel and Millet

together as Murrel Millet Mix is something unique to improve health status. Research

Collaboration has been established with Department of Food Science and Nutrition, University

of Agricultural Sciences, GKVK Campus, Bangalore to file patent for murrel millet mix.

22. Murrel Dhall Powder

Murrel Dhall Powder has been prepared using Murrel fish powder and other plant

products. It is a low fat food (Fat: 6.04%) and rich in calcium (800.8 mg/100g) and iron (229.5

mg/100g) with the assistance of Metayage IP Strategy Consulting Ltd, Coimbatore for filing.

This calcium and iron rich food is a health supplement for the malnourished children and old

aged people especially women. The patent application was filed on 25.10.2013.

b) Ready To Cook Products:

23. Murrel Fish Flakes:

Clean and steam cook the murrel fillet (1 Kg). Mix the tapioca flour (800 g), corn flour

(800 g) and soy bean flour (200 g) with required quantity of water and make into a paste. Add

salt, cumin seed powder (20 g) and little asafetida to the above mixture. Add steam boiled fillet

and mix properly. Pour into aluminium trays and spread evenly. Cook for 1 hour in steam and

cut into small pieces. Dry in oven maintained at 55 - 60ºC until moisture is reduced to about

10%. Store in polythene bags or tin containers for longer shelf life.

24. Murrel fish soup powder The murrel fillet of 250g was cleaned and steam cooked for 20 min. in pressure cooker.

To the cooked fillet, the ingredients viz: maida 75g, chopped onion 150g, cumin seed powder

15g, pepper powder 4g, vanaspathi 25g, curry masala powder 5g and trace amount of ascorbic

acid were added. The whole mass was then spread in aluminium trays and dried at 60°C until the

moisture was reduced to about 20%. The dehydrated mass was then powdered and mixed with

30g of skimmed milk powder and packed for marketing. Shelf life is about 6 months at room

temperature and over one year at refrigerated conditions. This product is easy to prepare, light

weight, protein rich and stable without refrigeration.

i. Utilization of Murrel Filleting Wastes:

As mentioned earlier, while filleting, the total yield of fillet is only 40%; however the

other wastes viz: head, viscera (intestine, caudal fin, pectoral fin, pelvic fin, and blood), skin and

bone constitute 23%, 22%, 8% and 7% respectively. All these unwanted organs were utilized

intelligently for the preparation / extraction of various useful products. The head and central

spine were utilized for soup powder preparation. The skin served as an interesting raw material

for gelatin and leather preparations. The viscera and diseased fish were used for manure

preparation to increase the fertility of garden soil.

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25. Murrel Fish Gelatin

For the preparation of gelatin, murrel fish skin was cleaned using tap water atleast three

times and drained. Then the fishes were frozen at -20ºC until use. After thawing overnight at

4°C, thawed skins were first cut into small pieces (about 2 to 3 cm) and then washed with

running tap water for 3 times. Skins were further cleaned with 0.8N sodium chloride (NaCl) (1:6

w/v) at 5ºC for 10 min and rinsed with abundant running tap water. Excess water was removed

by draining the cleaned skins and manual squeezing. The cleaned skins were treated with 0.2 N

sodium hydroxide (NaOH) (1:6 w/v) at room temperature for 30 min with constant stirring at

120 rpm and again rinsed with tap water (repeated 3 times). Skin were made to swell using 0.05

N acetic acid (1:6 w/v) at room temperature for three hours, rinsed with tap water (repeated 3

times) and then extracted with distilled water at 45ºC for 18 hours. The extracted gelatin

solutions were concentrated by rotary evaporator until moisture level reached less than 15%. The

extracted gelatin can be kept at room temperature for maximum two to three months.

Responsible Partner: CIFA, Bhubaneswar, Partner Institute

1. Ready- to-cook products

(a) Murrel Steaks: Murrel fish is rounded and an average fish of 600 g gives 20 nos of fish

steaks. Murrel steaks go very well for fish fry and curry preparation.

(b) Fingers, Chunks, Nuggets and Fillets: Deboned murrel flesh can be very well converted

into fish fingers, chunks and fillets. Fillets can be breaded and battered. They are shallow fried

and served. Chunks and nuggets are smaller pieces which are breaded, deep fried and served as

fast food.

(c) Minced Meat: Scrapings can be comminuted and made to minced meat. Spices and binders

are used and made into meat balls, cutlets and chops. Then they are deep fried and served as fast

food. Excellent fish fingers can be made from deboned murrel flesh.

2. Ready-to-eat products (d) Murrel Fish Soup: Murrel head and bones are used for hot and sour fish soups. Soups have

excellent flavour and are good appetizers.

(e) Murrel pickle: Murrel pickle was prepared using deboned meat. Two standard recipes for

hot and sweet/hot chilly murrel pickles were prepared. Pickling of murrel fish with vinegar,

mustard oil, green curry stuff, spices and condiments produced fairly acceptable murrel pickle.

The product can be safely stored for three months at ambient temperature without marked

deterioration in sensory quality. Murrel pickle is the highly acceptable and convenient fish

product of indigenous origin.

(f) Curry, Fingers, Fillets, Nuggets and Balls: From murrel processing, different ready to

cook products were developed. Ready to eat products viz., were prepared and evaluated by semi-

trained panelists using a nine point hedonic scale.

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Annexure III

Linkages and Collaborations

S. No. Linkages developed

(Name & Address of Organization)

Linkages developed by Lead Consortium: St.Xavier’s College (SXC) within India

1 PG & Research Department of Advanced Zoology and Biotechnology, Government Arts

College for Men, Nandhanam, Chennai – 600 035

2 Department of Zoology, Bharathiar University, Coimbatore – 641 046

3 National Bureau of Fish Genetics Research, Cochin Unit, CMFRI campus, Cochin-

682018 (Dr. A. Gopalakrishnan).

4 Dept. of Aquatic Biology and Fisheries, University of Kerala, Thiruvanthapuram – 695

581 (Dr. A. Biju Kumar).

5 Inland Fisheries Division, University of Agricultural Sciences, Bangalore

(Dr. D. Seenappa, Chief Scientific Officer - Inland Fisheries).

6 Fisheries Research and Information Centre,

Karnataka Veterinary Animal and Fisheries Science University, Bangalore – 560 094

7 National Fisheries Developmental Board (NFDB), Ameerpet, Hyderabad, Andhra

Pradesh.

8 Fish Metro Cash & Carry India Pvt. Ltd., Survey No. 133-140, Moosapet Village,

Balanagar Mandal, Ranga Reddi Dist. Hyderabad – 500072 (Mr. Laxhman Yaram).

9 State Fisheries Department, Hyderabad, Andra Pradesh.

10 State Fisheries Department, Bangalore, Karnataka.

11 Department of Life Sciences,Manipur University, Manipur -795003 (Dr. W.Vishvanath).

12 Department of Industrial Biotechnology, Karpagam University, Coimbatore - 641 021

(Dr. D. Teepica Priyadarshani).

13 Food Science and Nutrition Department, University of Agricultural Sciences, GKVK

Campus, Bangalore ( Dr.H.B. Shivaleela).

14 Manian Laboratories, Peelamedu, Coimbatore (Dr. Anusuya).

15 Sri Paramakalyani Centre for Environmental Studies, Manonmaniam Sundaranar

University, Tirunelveli (Dr. M. Arunachalam).

16 Department of Zoology, University of Madras, Chennai (Dr. P. Mullainadhan).

17 Division of Aquatic Environmental Management, University of Agricultural Sciences &

Technology of Kashmir, Jammu & Kashmir (Dr. Masood H. Balkhi).

18 Deaprtment of Biotechnology, Prathyusha Institute of Technology, Chennai

(Dr.Dhasarathan).

19 Department of Zoology, Scott Christian College, Nagercoil (Dr.Jayasuriya)

20 Central Institute of Brackishwater Aquaculture, Chennai (Dr. Thirunavukkarasu)

21 Aquaculture Foundation of India, Chennai (Dr.Sakthivel).

22 Comunity Action for Social Transformation, Poothathankudieruppu,

Thiruviruthanpuli (PO), Cheranmahadevi, Tirunelveli

23 DHAN Vayalagam (Tank) Foundation, Madurai (Mr.Madhan Mohan)

24 Tirunelveli Social Service Society, Krishnapuram, Tirunelveli

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25 Inner Wheel Club, Tirunelveli

Linkages developed by Lead Consortium: St.Xavier’s College (SXC) outside India

1 Dr. Roshada Hashim, School of Bioogical Sciences,

University of Sains Malaysia, Penang – 11800, Malaysia.

2 Dr. Nathar Khan, Ecotone Environmental Management SDN. BHD.

Suite 912, Block A, Kelana Centre Point,

3, Jalan SS7/19, Kelana Jaya, 47301 Petaling, Selangor, Malaysia

3 Dr. Tran Thi Thuy Ha,

Head of the Department, Genetics and Selection, Research Institute of Aquaculture No. 1

(RIA. 1), Dinh Bang- Tu Son- Bac Ninh, Vietnam

4 Dr. Samuel Appelbaum,

Head, The Bengis Center for Desert Aquaculture,

Ben-Gurion University of Negev, The Jacob Blaustein Institute of Desert Research,

Sede Bager Campus – 84990, Israel

5 Dr. Abdul Manan Mat Jais, Department of Biomedical Sciences, Faculty of Medicine

and Health Sciences, Universiti Putra Malaysia, Malaysia

6 Dr. M.Aminur Rahman, Research Fellow, Laboratory of Marine Biotechnology, Institute

of Bioscience (IBS), Universiti Putra Malaysia, Malaysia

7 Dr.C.V.Mohan, Aquatic Animal Health Specialist, Network of Aquaculture Centres in

Asia Pacific (NACA), Department of Fisheries, Kasetsart University Campus, Bangkok,

Thailand

8 Dr.Tan Heok Hui, Lecturer, Fish Taxonomy Unit, National University of Singapore,

Singapore.

9 Dr.K.Marimuthu, Faculty of Applied Sciences, AIMST University

10 Dr.Bui Minh Tam, Faculty of Aquaculture and Fisheries, Cantho University, Vietnam

11 Dr.Amrit N.Bart, Associate Professor, Asian Institute of Technology, Thailand

12 Dr.Jian G.Qin, Senior Lecturer in Aquaculture, School of Biological Sciences, Flinders

University, Australia

13 Dr.Andreas Graber, M.S. Environmental Sciences, ZHAW Zurich University of Applies

Sciences, Institute for Natural resources Sciences, Switzerland

14 Dr.Southa Faiza Brunner Wahab, Hoeschgasse, University of Zurich, Switzerland

15 Dr.Brian Thomson, Deputy Director, Global Communications Unit, IUCN, Switzerland

Linkages developed by Lead Consortium: St.Xavier’s College (SXC) with Fish Vendors

1 Mr.Arumugam, Melapalayam Fish market, Tirunelveli

2 Mr.Sethuhariharan, Tenkasi Fish Market

3 Mr.Allapitchai, Madurai Fish Market

4 Mr. M.A.Hammed & sons, Coimbatore

5 Mr.J.B.Hassain, Chennai Fish Market

6 Mr.Basheer, Shrimpex Aqua House, Ernakulam, Kerala

7 Mr. Nazeer, S.K.fish market, Trishur, Kerala

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8 Mr.Anoop, Adoor, Kerala

9 Mr. C.Sonnel Noronha, Thahazhy, Kerala

10 Mr. Ranson, Alapuzha, Kerala

11 Mr.Jefferson, Kanyakumari

12 Mr.Akleemkhan, Bhegam bazaar market

Linkages developed by Partner Institute: CIFA with Women SHGs

1 Narayani Narisakti Mahila Mahamandal (142 members), Aitalanga, Block-Balipatana,

Dist. Khurda

2 Beleswar Mahila Sangathan, Aitalanga, Dist.- Khurda

3 Maa Mangala WSHG, Aitalanga, Block- Balipatana, Dist. Khurda, Odisha

4 Maa Kalika WSHG, Block- Pubasasan, Dist. Khurda, Odisha

5 Maa Gelha Dei WSHG, Block- Pubasasan, Dist. Khurda,Odisha

6 Maa Mangala WSHG, Tota Sahi,Block-Pubasan, Dist. Khurda, Odisha

7 Maa Tarini WSHG, Village-Sorana, Dist-Khurda

8 Sri Bramheswaradev WSHG, Village-Sorana, Dist.- Khurda, Odisha

Linkages developed by Lead Consortium: St.Xavier’s College (SXC) with Women SHG

1 Mahalir SHG, Nagercoil, Tamil Nadu

Linkages developed by Partner Institute: CIFA with Men SHG

1 Maa Mahamangla Yubak Atmasahayak Gosthi, Tarapi, Balugaon, Odisha

Linkages developed by Partner Institute: CIFA with NGO

1 Unnayan NGO, Village- Ranghalo, Block- Nimapada, Dist.- Puri

Linkages developed by Partner Institute: CIFA with Government Organizations

1 KVK, CIFA, Khurda, Odisha

2 State Fisheries Department, Odisha

3 State Fisheries Department, Assam

4 NFDB, Hyderabad

5 State Fisheries Colleges from Karnataka, Orissa etc.

Linkages developed by Partner Institute: CIFA with Private Farmers

1 Mr. Amit Mohanty, Village-Birtung, Gop, Dist. -Puri Odisha

2 Mr. Brindavan Swain, Village- Nuagarh, PO. Barkul, Dist.-Khurda, Odisha

3 Mr. Sisirkant Biswal, At/PO- Kurujanga, Konark, Dist.- Puri,

4 Mr. Jalandhar Ghanta

Jamu Sahi, Village: Durga Prasad, Dasapalla, Dist. Nayagarh, Odisha

5 Mr. Braja Bihari Sahoo, Jamu Sahi, Dasapalla, Dist. Nayagarh, Odisha

6 Mr. Trinath Sahu, Jamu Sahi, Dasapalla, Dist. Nayagarh, Odisha

7 Mr. Saiban Kanhar, Village- Tanganadi, Block- Daspalla, Dist. Nayagarh, Odisha

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Linkages developed by Lead Consortium: St.Xavier’s College (SXC) with Private Farmers

1 Mr.Kiran, Nellore, Andhra Pradesh

2 Mr.Gurpreet Singh, Chhattisgarh, Punjab

3 Mr. Velusamy, 22 Periyasamykulam, Arasiyarpuram, Sankarankoil, Tvl district

4 Mr.Senbaharayappan, 51 Aathichudi street, Kallidaikurichi

5 Mr.Rahman, Akber Street, Melapalayam,Tirunelveli

6 Mr.Johnson, 748, Ramilas Nagar, Papankulam post, Nanguneri, Tirunelveli

7 Mr.Allah Pitchai, 21,Big street, Kadayanallur, Tirunelveli

8 Mr.Rafiq, 3rd

South street, Suthamalli, Tirunelveli

9 Mr.Jafar Saddik, Jawahar street, Tenkasi, Tirunelveli

10 Mr.Ramarajan, 248 Nelkottan seval, Tirunelveli District

11 S.Antonysamy, 2154, Veeramamuniver street, Tenkasi, Tirunelveli

12 Mr. Antony, Sinthamani village, Vasudevanallur, Tirunelveli

13 Mr.Tharmaraj, Kannapatti, Mettur post, Tenkasi, Tirunelveli

14 Mr. Ravindran, Elayankudi, Karaikal, Nagapatinam

15 Mr. Maria joseph, Anthoniyar kovil street, Akkarai vattam, Karaikal, Nagapattinam

16 Mr. Vinoth kumar, Puthagaram colony street, Needamangalam, Tiruvarur District

17 Mr.Jeevanantham, 99A Pasukada villi, Maninagaram, V.K Puram, Ambasamudram

18 Mr.Murugapandi, Veeralaperunselvi, Tirunelveli

19 Mr.Deivamani, Kazhuvathur post, Tiruvarur District

20 Mr. R.Ravi, Sogankudi post, Chengalpattu, Kanchipuram District

21 Mr.Rajasekar, 1/14 Kalayankuppam village, Ikkedu post, Tiruvallur District

22 Mr. Ravi sundaram, 15th

main road, SVS nagar, Valasaravakkam,Chennai-87

23 Mr. Manikkam, 18/72 mariyamman koil street, Ponnamma pettai, Salem

24 Mr.V.Nagarajan, N.R Biofarms, Siruvallai Post, Villupuram

25 Mr.K .Ramesh, Senthil farms , Mugaiyur , Tirukovilur Taluk, Vilupuram

26 Mr.Hari, No.2 Savadi street, Pallavaram, Chennai

27 Mr.Naresh, 124,NH ottakuthar Street, Maraimalainagar, Kanchipuram District

28 Mr.Natrajan, 5th

kovil street, Selarayal, Chennai-51

29 Mr.Rafeeq, 3 Maggamma nagar, Viyasaraja nagar, Srirangam, Trichy

30 Mr.Illaperumal, ¾ Periyar street, Nagathy, Villupuram

31 Mr.Sivakumar, Malaipatti village, Arupukottai Tk, Virudhunagar

32 Mr.Sekar, Cavary patti post, Sangakiri TK, Salem

33 Mr. Thirumavalavan, 11/59 3rd

Main road, Kasthuri nagar, Adayar, Chennai

34 Mr. Venkatesan, 14, Cavureey college road, Annamalai Nagar, Trichy-12

35 Mr. Stephen Sampath, Mathakoil street, Eervadi, Tirunelveli

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36 T.Arun Raj,Tamilar street,Keelapavoor(PO), Alangulam(T.K.)-627806.

37 M.Kulandai Velu,8A,Sudalai Madaswamy Kovil street,Vadakkoor,Gopalasamuthiram-

627407,Tirunelveli.

38 P.Senthilvel, 4/436, Keelanatham, Vadakkoor, Keelanatham(Post), Tirunelveli-627353

39 M.Muralidharan, Plot No:16, Zion Town,Near IRT polytechnic ,

Konganthanparai PO,Trivandrum Road, T irunelveli-627007.

40 Mr.John Winslin, 35, Valluvar Nagar Extension, Near J.K.Hospital, Reddiyar patti,

Tirunelveli-7

41 S.Joseph Jeyaraj,5,Bharath Nagar, Chettiapatty Main Road,

Ramachandra Nagar, Edamalaipatty pudur PO

42 Fedrick Nickson, 36, Keela Kodical street, Kulithalai, Karur Dt.

43 S.Mani Mozhian, 28,Subbiah puram, Tuticorin-628003.

44 P.Rajakumar, 1/185, Kamaraj street, Andipatti , Alangulam Tk.

45 Bala Krishnan, 93 G/9, Teachers colony, 2nd

Street, Tuticorin-8

46 K.Bala Murugan, 1/23 Vinayagar koil street, Narasingonur, Vembe (po), Villupuram.

47 D.Rama Krishnan, L 38,TN HB colony, Stage-11, VM Chathram,

48 G.Sudhan, 915K, Brindhavan Nagar,3rd

street, KTC Nagar

49 A.Muthiah, 25/12B3, St.Paul’s Road, Near A.R.Line, Palayamkottai, Tirunelveli-2

50 K.Sabari Raj, 11/66, C-1 Kongu Nagar, Vadasandhur, Angalukurichi post

Pollachi-642007

51 S.Kareem, 336/1,First Street, Shanthi Nagar, Palayamkottai, Tirunelveli

52 Collector Residence, High Ground , Tirunelveli

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Annexure IV

Selected Success Stories

Success Story-I:

One trainee M/S. C. Manickaraj, Agaram

Vallanadu Post, Tuticorin Dt., Tamilnadu was

selected for induced breeding attempts. In total, 12

matured females and 24 male fishes weighing from

650 g to 900 g were selected for hormonal

injection using HCG hormone. The hormone

injected brood fish were introduced into breeding

pits (6 x 4 x 2.5 feet) containing aquatic

macrophytes Eichornia crassipes for hiding

purposes. Ten sets showed positive response and

60,000 eggs were laid approximately (Mean 6,000

eggs/set). At 85% hatching rate 50,000 hatchlings were totally found in the breeding pits. He got

40,000 fingerlings after a period of 45 days at a survival rate of 80 %. He introduced 5,000

fingerlings into a culture pond of 1 acre and remaining 35,000 fingerlings he sold to other fish

farmers. The fishes have attained a mean size of 700g after a culture period of eight months.

Success story II:

The District Collector of Tirunelveli was approached

to excavate an earthen pond as a model in his residence. A

rectangular pond 60 ft x 40 ft x 6 ft was excavated at the

backyard. 400 fingerlings of C. striatus weighing 5.5 ±0.92 g

were introduced into the pond. The fingerlings were fed with

semi-moist formulated feed at 5% of body weight twice a

day. After three months they were exclusively fed on minced

chicken intestine at 10 % of the body weight. During the ten

months culture period water was completely drained only

once after six months and all the murrels were harvested and

length and weight were recorded. No dead fishes were noticed. From this experience, it is

possible to suggest that cannibalism can be avoided when food was supplied ad libidum.

Although initially 400 fingerlings were introduced, more than 200 fingerlings died within a

month due to poor water quality. After the 10 months culture period, 80 table sized fishes

weighing a total of 60 kg and ranging 620-785 g were harvested.

This technology was recommended to housewives to culture murrels in their backyards,

thereby providing valuable animal protein for the household and earning them extra income

through sale of surplus fish. From a minimum area of 600 sq feet one could produce 60-70 table

sized striped murrels (60Kg).

Success story III.

At CARE Aquafarm, 100 fingerlings of C.

striatus weighing 5.4 ±0.79 g were introduced into pond

5 (15m × 5m × 1m). The fingerlings were fed with semi-

moist formulated feed at 5% of body weight twice a day.

After three months, they were exclusively fed on minced

chicken intestine and formulated feed at 10% body

weight. Sampling was done after every three months to

study survival and growth rate. With 76% survival, 76 C.

striatus fishes were harvested after ten month culture period ranging in size from 500 – 585 g (

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52 kg ).

Success story IV.

Mr.M.Thaivamani and M.Kottalam of Kaluvathur Post, Mannarkudi Taluk, Tiruvarur

District, Tamilnadu, started murrel culture on 4th

Dec 2009. They started murrel farming in a

model pond (60 feet X 40 feet X 3 feet) and they maintained the pond with lake, river & bore

water. They added 200 Kg cow dung and Lime before stocking. 500 fingerlings of C.striatus

having 30g initial weight & 7.5 cm length were introduced into the pond, and were fed with

semi-moist formulated feed (70% fish meal, 10% wheat flour, 5% rice flour, 5% tapioca flour &

10% soya flour) at 5% body weight twice a day. After three months they were exclusively fed on

minced chicken intestine at 10% of body weight. During the ten month culture period water was

completely drained only once after six months and all the murrels were harvested and length &

weight were recorded. No diseased or dead fishes were noticed.

Although initially 500 fingerlings were introduced, more than 200 murrel fishes were

escaped due to heavy rain. After 10 months culture period, 290 table size fishes weighing a total

of 300 Kg ranging 700-1000g were harvested and sold on the spot at a rate of Rs. 250/Kg.

Success story V

Mr.K.Jeevanantham, V.K.Puram, Ambasamudram Taluk, Tirunelveli District, Tamilnadu

started murrel culture on July 2009. The pond was

stocked with 500 advanced fingerlings of C.striatus

14g initial weight & 4.5 cm length. The fingerlings

were fed with semi-moist formulated feed (70% fish

meal, 10% wheat flour, 5% rice flour, 5% tapioca flour

& 10% Soya flour) at 5% body weight twice a day.

After three months they were exclusively fed on

minced chicken intestine at 5% and minced trash fish

5% of the body weight. Since the pond is a natural one naturally available live trash fishes are

present in large number. During the ten months culture period water was completely drained

only once after six months and all the murrels were harvested and length & weight were

recorded. No diseased or dead fishes were noticed.

Although initially 500 fingerlings were introduced, After 10 months culture period, 200

table size fishes weighing a total of 200 Kg ranging 700-1Kg were harvested and sold on the spot

at Rs.250/Kg.

Success story VI

Mr.Hari, Pallavaram, Chennai started murrel farming

in November 2010. He released 800 murrel fingerlings in an

area of 0.039 Ha. Prior to introduction, the pond was

deweeded. The fingerlings were fed with trash fish at 5%

body weight regularly. During the nine month culture period

50% water was drained every month. Murrels with EUS

symptoms noticed during winter and proper herbal treatment

was given by CARE research team. A total of 80 Kg murrels

was harvested after nine months culture period. A part of

harvested murrels which weigh above 900 g were sold at the farm itself (Rs 280/Kg) and the

remaining fishes were kept in a separate pond for breeding purposes.

Success story VII

One progressive fish farmer Mr.Ravi, Thalembedu village; Kanchipuram District strated

murrel farming in an area of 42.42 Ha. The pond was cleared from weeds and 2000 Kg cow dung

& lime were added before stocking, 12,000 advanced fingerlings of 10 cm length & 12g initial

weight were introduced into the pond during July 2010. Since the pond is a natural one, live trash

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fishes are present in large number; they were exclusively fed on trash fish at 5% body weight. In

due course of culture, the fish got EUS during winter session and treated using herbs with the

help of research team of CARE. A total of 4000 Kg murrels harvested. A part of the produce

weighing 900g – 1Kg was sold at a rate of Rs 200/Kg.

Success story VIII

Mr. Naresh, Marimalainagar, Kanchipuram District, Tamilnadu started murrel culture in

August 2010. He started murrel farming in an area of 0.32 Ha. He added 2 tons of cow dung &

lime before stocking. 3,000 fingerlings of C.striatus having 2.5 inch length & 8g weight were

introduced into the pond and fed on live tilapia fingerlings which were already stocked before

introduction of murrel fingerlings. After ten months of culture period, 1367 Kg ranging 750g –

1Kg were harvested and sold at a rate of Rs100/Kg.

Success story IX

Mr.Rafeeq, Srirangam, Trichy District Tamilnadu started murrel culture in an area of

0.24 Ha in July 2010. He added 250Kg cow dung & lime before stocking. 300 advanced

fingerlings of C.striatus having 150g weight were fed with boiled chicken intestine & semi moist

formulated feed (fishmeal 70%, Wheat flour 10%, Rice flour 5%, Soy flour 10% & Tapioca 5%)

at 5% body weight. After 9 months, 200Kg of table size fishes were harvested & sold at a rate of

Rs 250/Kg.

Success story X

Mr. V.Nagarajan, Siruvallai, Villupruam District, Tamilnadu started murrel culture in an

area of 1.25Ha in August 2010; pond was prestocked with cow dung & organic fertilizers. After

fertilization, pond was stocked with 4800 advanced fingerlings having average length of 12cm &

weights of 20g were introduced. The fingerlings were fed with minced trash fish & semi moist

formulated feed at 5% body weight twice a day. During the culture period the pond water was

changed every month. After 9 month culture period, about 650 Kg ranging 750g – 1Kg murrel

fishes were harvested and sold at a rate of Rs. 320/Kg.

Success story XI

Mr.B.G.Rajasekar, Kalyanakuppam, Tiruvallur District started murrel culture in an area

of 0.18 Ha in August 2010, the pond was stocked with 5000 advanced fingerlings of C.striatus

having length of 6-8 cm & weight of 10g were introduced into the pond. Before stocking

fingerlings, the culture pond was stocked with Tilapia brooders, after solid 1 month time, the

tilapia fingerlings were produced in the pond. Using this suitable situation, the murrel fingerlings

were introduced. Meanwile the farmer didn’t supply any semi-moist feed for murrel culture.

Moreover he regularly supplied groundnut oil cake, rice bran & boiled rice for tilapia. This kind

of approach was very helpful to maintain a study growth of murrel fish. Initially 5000 fingerlings

introduced, after 9 month culture period about 750g – 1.25Kg murrel fishes were harvested &

sold at a rate of Rs 200/Kg.

Success story XII

Mr.Elumalai, Tirukalukundram, Kanchipuram

District, Tamilnadu started murrel culture in July 2010.

This farmer is a business person and owner of a

provision store. He took five community ponds of an

area of 147.7 ha under lease; they were deweeded &

prepared for murrel culture. Ponds were stocked with

67,000 advanced fingerlings having an average length

of 5 cm & weight of 8g of C.striatus. Fishes were fed

with Carp & Tilapia fingerlings by introducing the

brooders of the above mentioned fish varieties before

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Women from SHG releasing murrel

seed in their pond

one month of introduction of murrel fingerlings. After 10 months, 8000 Kg fish ranging from

750g to 1Kg were harvested and sold at a rate of Rs 200/Kg.

Success story XIII

Arasan Aquafarm, Araikulam, Tirunelveli

District was selected and trained for both murrel seed

production and table size fish production by CARE,

St.Xavier’s College, Palayamkottai. This Aquafarm

has enough larviculture facilities and plankton culture

units. Research team of CARE produced 5000 seeds

of C. striatus in June 2011 and were used for

Growout culture. For the first 4 months, fingerlings

were fed with semimoist feed, 5% body weight

suggested by CARE. After 4 months, they were fed with chicken intestine added semi-moist

feed. A total of 45 Kg murrels were harvested after nine months culture period and fishes were

stocked in a separate pond to maintain the brood stock for further breeding attempts.

Success story XIV

One progressive fish farmer Mr. Philip Abraham, Kaliyavoor, Tuticorin District started

murrel culture in January 2011. 300 fingerlings of an average size of 2.5 cm length & 5 g weight

were introduced into the pond. Initially they were fed with semimoist feed with chicken liver. At

that time, he maintained plankton culture in a separate cement tank; he added plankton weekly

twice to the pond to enhance the live feed growth in the pond. After 4 months, fish fed with

finely chopped chicken intestine. After 10 months, 45 Kg of murrels were harvested and sold at

Melapalayam fish market at the price of Rs.260/Kg.

Success stories by CIFA Bhubaneshwar:

Success Story-I:

During the survey a few potential women self help group (WSHG) and individual fish farmers

have been identified for murrel farming. Among them one of the Women Self Help Group

named Narayani Nari Shakti Mahila Mandal , Village-

Aitalanga, P.O. - Aitalanga, Block- Balip[atna, Dist. –

Khurda was selected for murrel farming as they were

very enthusiastic to start murrel farming. The group

comprises of 144 members. The WSHG was doing other

agricultural activities including carp fish farming but they

were unaware of the murrel fish farming. CIFA provided

training on murrel

culture and guided

them for its

farming. They

leased out a community pond of area 300 m2, which was

infested with weeds.

The pond was de-weeded and prepared for murrels culture.

They have been given three days training at CIFA, during

which the biology, feed preparation and feeding, breeding,

rearing of larvae and grow out culture for murrel were

taught both theoretically as well as practically. A training manual was provided in Oriya to make

them understand the technology easily. Pond was stocked with advanced fingerlings (avg. wt

68.5gm) of Channa striatus (150nos.) and C marulius(50nos). Regular visits were made by the

CIFA NAIP team with selected WSHG

members

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Murrel seed being released in pond of

the adopted NGO, Unnayan

CIFA NAIP teams to monitor the growth and health status of the fish as well as to boost their

confidence by giving technical support. In due course of culture, once the fish got ulcer disease

during winter. It could be checked with proper treatment with the help of CIFA. Due to harsh

summer the water level of pond reduced to its minimum and hence early harvesting was done.

The total of 60 Kg murrels was harvested in 5 months of culture. The rate of production was

4800 Kg/ha/yr from the community pond. A part of the produce weighing 900- 1000 g was sold

on farm @ Rs 80/Kg. The remaining fishes were kept in a separate pond for development of

brooders.

This production level was very much encouraging for the WSHG and shown their interest

to continue the murrel culture. They took help from CIFA NAIP team for the maintenance of

brood stock. The brood fish were fed with boiled trash fish and poultry offal @ 3-5% of body

weight to maintain a healthy reproductive status. Other managerial practices were also followed

to maintain good water quality. Some emergent aquatic plants were allowed to grow in the

margin of the pond. This provided the natural environment for the murrels. At the onset of

monsoon the brood fishes spawned eight batches of spawn, comprising of 2-3 thousands per

batch were obtained. The spawn were collected and reared separately with proper feeding

regiments. They followed the practice of segregation and separate rearing as advised by the

CIFA. By following this method, they got very high survivability. The total of 15,000 fingerlings

was produced.

Success Story-II:

One progressive NGO named Unnayan, At-Ranghalo, Dist-Puri, was also selected for the

murrel farming. This NGO involved in variety of

agricultural activities, including aquaculture. The

members were given three days training on murrel seed

production, rearing and culture at CIFA. They were

interested to include a new candidate freshwater

species ie. Channa striatus (murrel) to increase their

income. Since they were not familiar with murrel

farming, they had started with a small pond (200m2).

The pond was highly infested with weeds and it was

not in use. They cleared the weeds and did pond

preparation before stocking. CIFA provided them 100

numbers of advanced fingerlings (Avg. wt. 76gm) for

stocking in the pond. Fishes were fed with boiled and

chopped poultry viscera. Technical support was

provided to build confidence in them. After 10 months of culture the production was 47 kg. The

production rate was 2,835Kg/ha/yr. Though production is comparatively less but it was sufficient

to trigger their interest for the murrel farming. Apart from culture, they could able to breed the

fish in natural environment with the help of NAIP team of CIFA. They have collected 2,500

fingerlings and distributed to a few interested farmers.

The idea to adopt WSHG and NGO was to spread the technology by their members in a bigger

way. Their initial achievement with regard to culture of murrel has encouraged them to adopt

murrel farming. After the awareness programme and seeing the success, many rural farmers are

coming to CARE and CIFA for the help for murrel farming. The ponds which cannot be used for

carp may be utilized for murrel culture. So, the murrel farming has added advantage to increase

fish production by utilizing unsuitable and derelict water bodies and thereby improvement of

rural livelihood.