FINAL REPORT LUE IMPACT Biomolecules project

1

FINAL REPORT LUE IMPACT Biomolecules project

FINAL REPORT

2021

LUE IMPACT Biomolecules project

1


FINAL REPORT

2021

LUE IMPACT Biomolecules project

The LUE IMPACT Biomolecules project encompassed all steps required for exploitation of biomolecules, from initial production, to elaboration and biological testing, to final application

2 2

The LUE IMPACT Biomolecules project encompassed all steps required for exploitation of biomolecules, from initial production, to elaboration and biological testing, to final application

2

3


Table of contents

Main research outcomes p. 6

Networking and structuring of the research p. 16

Key project indicators p. 20

Hiring of PhDs/Post-doctoral researchers/Others p. 21

Annexe 1 : Promoting Biomolecules p. 24

Annexe 2 : List of publications, oral communications, patents etc. p. 26

Introduction p. 5

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Table of contents

444

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The LUE IMPACT Biomolecules project encompassed all steps required for exploitation of biomolecules, from initial production, to elaboration and biological testing, to final application, with the goal of identifying novel com-pounds exhibiting anti-proliferative, antioxidant and/or anti-inflammatory activities. One of the initial objectives was to establish a collaborative network encompassing 17 laboratories (Calbinotox, CITHEFOR, CRAN, DCAC, DynAMic, IAM, IMoPA, L2CM, LAE, LCOMS, LCPM, LERMaB, LIBio, LORIA, LRGP, SIMPA, URAFPA) within the Lorraine region (120 total researchers), in close association with local, national and international industry spanning the entire value chain (presently 17 companies: Abolis Biotechnologies, Avril, BASF, Biolie, Cellengo, Cookal, Extratex, Fluid’air, Genialis, IEA, Laboratoires Spiral, Myrissi, Norske Skog, PAT, Salveco, Septeos, St Hubert and Tereos). This strategy resulted in the funding of 75 projects covering both theoretical and applied science, for a global budget of €9 M (2017-2021). Together, these research activities resulted in more than 200 research outputs (123 articles in peer-reviewed journals and 79 communications at international conferences). One national and two inter-national conferences were also organized within the project framework. The main outcomes of the LUE IMPACT Biomolecules project are described below.

5


5


The LUE IMPACT Biomolecules project encompassed all steps required for exploitation of biomolecules, from initial production, to elaboration and biological testing, to final application, with the goal of identifying novel com-pounds exhibiting anti-proliferative, antioxidant and/or anti-inflammatory activities. One of the initial objectives was to establish a collaborative network encompassing 17 laboratories (Calbinotox, CITHEFOR, CRAN, DCAC, DynAMic, IAM, IMoPA, L2CM, LAE, LCOMS, LCPM, LERMaB, LIBio, LORIA, LRGP, SIMPA, URAFPA) within the Lorraine region (120 total researchers), in close association with local, national and international industry spanning the entire value chain (presently 17 companies: Abolis Biotechnologies, Avril, BASF, Biolie, Cellengo, Cookal, Extratex, Fluid’air, Genialis, IEA, Laboratoires Spiral, Myrissi, Norske Skog, PAT, Salveco, Septeos, St Hubert and Tereos). This strategy resulted in the funding of 75 projects covering both theoretical and applied science, for a global budget of €9 M (2017-2021). Together, these research activities resulted in more than 200 research outputs (123 articles in peer-reviewed journals and 79 communications at international conferences). One national and two inter-national conferences were also organized within the project framework. The main outcomes of the LUE IMPACT Biomolecules project are described below.

6

Main research outcomes

Discovery of New Active BiomoleculesTowards the goal of discovering new molecular entities, the project exploited novel biological interactions (principally between plants and microorganisms), to induce metabolites synthesis. This strategy (Workpackage (WP) 1) was deployed using some 1000 species of bacteria and plants.

Specifically:

• Co-culturing between soil bacteria (Streptomyces) and fungi was developed as a

mean to mimic biotic stimuli encountered during natural growth conditions and to

elicit novel compound production. 72 different Streptomyces/fungal interactions

were studied and compared to the respective mono-cultures. Co-culturing was shown

to have a strong impact on the metabolic expression of each partner and resulted in

the biosynthesis of specific compounds. This study opened a promising avenue of

research for exploring and exploiting the metabolic capacities of both Streptomyces

and fungi. (patenting in progress)

• Endophytes isolated from plants were identified and several of them exhibited pro-

mising activity against the proliferation of the filamentous fungi Fusarium on wheat

(154 isolates tested). 10 of the resulting strains are now under intensive investigation

for use as biocontrol agents on cereals fields. (patenting in progress)

• Colonies of endophytes (800) were cultivated and their metabolism studied to identify

new biosynthetic pathways (principally alkaloids). Three of the hit pathways have been

shown to produce active biomolecules (artepillin C, drupanine and 3,5-diCQA) using

a metabolic engineering strategy in recombinant yeast. The production process for

one of these molecules has been patented.

6

Main research outcomes

Discovery of New Active BiomoleculesTowards the goal of discovering new molecular entities, the project exploited novel biological interactions (principally between plants and microorganisms), to induce metabolites synthesis. This strategy (Workpackage (WP) 1) was deployed using some 1000 species of bacteria and plants.

Specifically:

• Co-culturing between soil bacteria (Streptomyces) and fungi was developed as a

mean to mimic biotic stimuli encountered during natural growth conditions and to

elicit novel compound production. 72 different Streptomyces/fungal interactions

were studied and compared to the respective mono-cultures. Co-culturing was shown

to have a strong impact on the metabolic expression of each partner and resulted in

the biosynthesis of specific compounds. This study opened a promising avenue of

research for exploring and exploiting the metabolic capacities of both Streptomyces

and fungi. (patenting in progress)

• Endophytes isolated from plants were identified and several of them exhibited pro-

mising activity against the proliferation of the filamentous fungi Fusarium on wheat

(154 isolates tested). 10 of the resulting strains are now under intensive investigation

for use as biocontrol agents on cereals fields. (patenting in progress)

• Colonies of endophytes (800) were cultivated and their metabolism studied to identify

new biosynthetic pathways (principally alkaloids). Three of the hit pathways have been

shown to produce active biomolecules (artepillin C, drupanine and 3,5-diCQA) using

a metabolic engineering strategy in recombinant yeast. The production process for

one of these molecules has been patented.


Production of biomolecules was accelerated through deliberate stressing of plants du-

ring culturing. To support this work, €700k was invested (2018-2019) to purchase eight

state-of-the art ‘phytotronic chambers’ (cofinanced by CPER Agrovalor), which allow

precise control of specific stress conditions (temperature, light, humidity, presence of

biological aggressors, etc.). This work resulted in the identification of a potent inhibitor

of bacterial growth. Four further compounds are under investigation, notably including

biomolecules from truffles which exhibit strong antiproliferative (antitumor) activities. In

addition, an emerging project has explored temporal variations in metabolic signatures

of plants exposed to stresses, including analysis of green leaf volatile compounds and

soluble metabolites such as glucosinolates. On-going analyses of these data will be used

to inform strategies to induce plant defenses as an approach to biocontrol.

• Finally, a mass spectrometry database has been created (DataNat, version 1, FR.001.48

0019.000.S.P.2020.000.10300), based on the work in WP1 and TA2, which is registered

with APP, a European organization specializing in the protection of software, mobile ap-

plications, databases, websites and strategic data. The database contains data obtained

from approximately 100 biomolecules of natural origin (commercial standards or com-

pounds isolated by the L2CM laboratory), and encompasses various molecular classes

including organic acids (70%), alkaloids (20%), and others (10% including coumarins, an-

thraquinones, sugars…). The specific data include the retention time of each compound

and MS/MS spectra recorded in positive and/or in negative ion mode, using the expe-

rimental conditions developed by the L2CM. A new version (version 2) covering more

compounds will be released at the end of 2021, and will additionally include NMR data.

Globally, as a direct result of this project, five active biomolecules have entered into the

patenting process, as well as bioreactor-scale production, to allow for their industrial

deployment in biocontrol and/or medical applications. In addition, the strong links esta-

blished between four industry members (Agrauxine, Lorca, BASF and PAT) and academic

researchers, led to the founding of a specific UL chair (Chaire de ‘Bio4solutions’). This

chair currently develops joint actions to promote biosolutions emerging from the LUE

IMPACT biomolecules project for agricultural applications.

77

FINAL REPORT LUE IMPACT Biomolecules projectFINAL REPORT LUE IMPACT Biomolecules project

Production of biomolecules was accelerated through deliberate stressing of plants du-

ring culturing. To support this work, €700k was invested (2018-2019) to purchase eight

state-of-the art ‘phytotronic chambers’ (cofinanced by CPER Agrovalor), which allow

precise control of specific stress conditions (temperature, light, humidity, presence of

biological aggressors, etc.). This work resulted in the identification of a potent inhibitor

of bacterial growth. Four further compounds are under investigation, notably including

biomolecules from truffles which exhibit strong antiproliferative (antitumor) activities. In

addition, an emerging project has explored temporal variations in metabolic signatures

of plants exposed to stresses, including analysis of green leaf volatile compounds and

soluble metabolites such as glucosinolates. On-going analyses of these data will be used

to inform strategies to induce plant defenses as an approach to biocontrol.

• Finally, a mass spectrometry database has been created (DataNat, version 1, FR.001.48

0019.000.S.P.2020.000.10300), based on the work in WP1 and TA2, which is registered

with APP, a European organization specializing in the protection of software, mobile ap-

plications, databases, websites and strategic data. The database contains data obtained

from approximately 100 biomolecules of natural origin (commercial standards or com-

pounds isolated by the L2CM laboratory), and encompasses various molecular classes

including organic acids (70%), alkaloids (20%), and others (10% including coumarins, an-

thraquinones, sugars…). The specific data include the retention time of each compound

and MS/MS spectra recorded in positive and/or in negative ion mode, using the expe-

rimental conditions developed by the L2CM. A new version (version 2) covering more

compounds will be released at the end of 2021, and will additionally include NMR data.

Globally, as a direct result of this project, five active biomolecules have entered into the

patenting process, as well as bioreactor-scale production, to allow for their industrial

deployment in biocontrol and/or medical applications. In addition, the strong links esta-

blished between four industry members (Agrauxine, Lorca, BASF and PAT) and academic

researchers, led to the founding of a specific UL chair (Chaire de ‘Bio4solutions’). This

chair currently develops joint actions to promote biosolutions emerging from the LUE

IMPACT biomolecules project for agricultural applications.

7

8

In parallel with these activities for discovering high added value biomolecules, work

has been carried out on three model types of biomolecule, with the goal of obtaining

rapid proof-of-principle for approaches aiming to optimize the therapeutic potencies

of newly-discovered entities. This work was organized into three transversal actions

(TAs - targeted biological activity): polyketides (TA1 - antiproliferative), polyphenols

(TA2 - antioxidant & anti-inflammatory) and metal chelating peptides (TA3 - antioxidant).

• TA1: Polyketides were chosen for their outstanding medical interest notably

in the anti-infective and anti-cancer arenas, and as a model to develop synthetic bio-

logy approaches. Novel polyketides have already been generated by eliminating select

portions of the molecules by genetic engineering or by diversifying a specific side

chain using a ‘mutasynthesis’ approach. Patent discussions on the novel compounds

are in progress with the SATT SAYENS.

• TA2: Polyphenols of interest were sourced from wood, plants and fungi, and ef-

ficient protocols developed (fractionation, hydrolysis and/or purification)

to provide rich extracts for downstream analysis (see Step 3, below). Of note, a parti-

cular functionalized polyphenolic extract exhibiting high antioxidant activity is being

shepherded towards practical application by the regional business accelerator (SATT

SAYENS). A second polyphenolic extract is now a candidate for clinical application

towards restoring both bone density and immunity.

• TA3: The overall aims of this action were to develop novel ways to generate, fractionate,

rapidly evaluate and purify metal chelating peptides. As a result, enzymatic hydroly-

sis processes were elaborated which allow for production of peptides from natural

resources. Rapid extraction of the most active peptides was then accomplished via

novel affinity-based protocols (IMAC-MS, fluorimetry and SPR). These innovative me-

thods are now being applied to large numbers of extracts containing metal chelating

peptides, greatly facilitating their purification and characterization.

8

In parallel with these activities for discovering high added value biomolecules, work

has been carried out on three model types of biomolecule, with the goal of obtaining

rapid proof-of-principle for approaches aiming to optimize the therapeutic potencies

of newly-discovered entities. This work was organized into three transversal actions

(TAs - targeted biological activity): polyketides (TA1 - antiproliferative), polyphenols

(TA2 - antioxidant & anti-inflammatory) and metal chelating peptides (TA3 - antioxidant).

• TA1: Polyketides were chosen for their outstanding medical interest notably

in the anti-infective and anti-cancer arenas, and as a model to develop synthetic bio-

logy approaches. Novel polyketides have already been generated by eliminating select

portions of the molecules by genetic engineering or by diversifying a specific side

chain using a ‘mutasynthesis’ approach. Patent discussions on the novel compounds

are in progress with the SATT SAYENS.

• TA2: Polyphenols of interest were sourced from wood, plants and fungi, and ef-

ficient protocols developed (fractionation, hydrolysis and/or purification)

to provide rich extracts for downstream analysis (see Step 3, below). Of note, a parti-

cular functionalized polyphenolic extract exhibiting high antioxidant activity is being

shepherded towards practical application by the regional business accelerator (SATT

SAYENS). A second polyphenolic extract is now a candidate for clinical application

towards restoring both bone density and immunity.

• TA3: The overall aims of this action were to develop novel ways to generate, fractionate,

rapidly evaluate and purify metal chelating peptides. As a result, enzymatic hydroly-

sis processes were elaborated which allow for production of peptides from natural

resources. Rapid extraction of the most active peptides was then accomplished via

novel affinity-based protocols (IMAC-MS, fluorimetry and SPR). These innovative me-

thods are now being applied to large numbers of extracts containing metal chelating

peptides, greatly facilitating their purification and characterization.

9


For biocontrol, pharmaceutical and food applications, it is often necessary to adapt molecular properties to meet industrial requi-rements via functionalization and/or stabilization. Workpackage 2 (WP2) addressed both of these key aspects of hit development.

Specifically, enzymatic processes have been established in favor of chemical methods

to functionalize biomolecules, with the overall aim of limiting environmental impacts.

Results of note include identifying acylation as an effective means to increase solubility

and bioavailability, and applying this approach to the efficient and selective modification

of antioxidant polyphenols. Progress has also been made towards optimizing these en-

zyme-based methods via molecular modelling and docking. Indeed, substantial investment

has been made (with CPER funds and 3BR project) in developing a molecular modelling

platform integrating the latest software, to permit both rapid calculation and simulation.

In complementary work, enzyme immobilization has been used to build scalable processes

adapted to industrial use. Stability as well as recycling of the enzymes have been signi-

ficantly improved by immobilization in a silica matrix. The resulting patented process is

now applied to newly-extracted biomolecules, in collaboration with an industrial partner.

A additional approach to functionalization is vectorization. In this strategy, the biomolecule

is not chemically modified, but encapsulated in an external matrix in order to improve

its bioavailability and stability. Vectorization also allows for targeting release of active

agents to specific locations in the human body. The LUE IMPACT Biomolecules project has

focused on optimization of two types of vector: liposomes and matrices. Newly designed

liposomes, for example, have been shown to facilitate efficient delivery to brain cells,

due to their innovative composition which associates polyunsaturated fatty acids (PUFA

vector) and biomolecules. As PUFAs are readily absorbed by the neuronal network, the

result is effective delivery of the cargo, and indeed, this approach has been successfully

used to transport PUFAs to neurons in an experimental model of Alzheimer’s disease.

In terms of matrix-type vectors, the project has explored the use of chitosans and polyphe-

nols as materials for protecting biomolecules during the digestive process, thus achieving

controlled release in the intestine, which results in improved absorption. A novel, layer-

upon-layer vector architecture was developed, which offers both efficient protection and

targeted release of cargo molecules. In parallel, a mathematical model relating vector

structure and release kinetics is being developed, in order to facilitate conception of

future generations of vectors. Finally, industry-supported vectorization approach were

explored with the aim of using milk proteins alginate and chitosan for encapsulation.

Functionalization and Vectorization of Biomolecules

9


For biocontrol, pharmaceutical and food applications, it is often necessary to adapt molecular properties to meet industrial requi-rements via functionalization and/or stabilization. Workpackage 2 (WP2) addressed both of these key aspects of hit development.

Specifically, enzymatic processes have been established in favor of chemical methods

to functionalize biomolecules, with the overall aim of limiting environmental impacts.

Results of note include identifying acylation as an effective means to increase solubility

and bioavailability, and applying this approach to the efficient and selective modification

of antioxidant polyphenols. Progress has also been made towards optimizing these en-

zyme-based methods via molecular modelling and docking. Indeed, substantial investment

has been made (with CPER funds and 3BR project) in developing a molecular modelling

platform integrating the latest software, to permit both rapid calculation and simulation.

In complementary work, enzyme immobilization has been used to build scalable processes

adapted to industrial use. Stability as well as recycling of the enzymes have been signi-

ficantly improved by immobilization in a silica matrix. The resulting patented process is

now applied to newly-extracted biomolecules, in collaboration with an industrial partner.

A additional approach to functionalization is vectorization. In this strategy, the biomolecule

is not chemically modified, but encapsulated in an external matrix in order to improve

its bioavailability and stability. Vectorization also allows for targeting release of active

agents to specific locations in the human body. The LUE IMPACT Biomolecules project has

focused on optimization of two types of vector: liposomes and matrices. Newly designed

liposomes, for example, have been shown to facilitate efficient delivery to brain cells,

due to their innovative composition which associates polyunsaturated fatty acids (PUFA

vector) and biomolecules. As PUFAs are readily absorbed by the neuronal network, the

result is effective delivery of the cargo, and indeed, this approach has been successfully

used to transport PUFAs to neurons in an experimental model of Alzheimer’s disease.

In terms of matrix-type vectors, the project has explored the use of chitosans and polyphe-

nols as materials for protecting biomolecules during the digestive process, thus achieving

controlled release in the intestine, which results in improved absorption. A novel, layer-

upon-layer vector architecture was developed, which offers both efficient protection and

targeted release of cargo molecules. In parallel, a mathematical model relating vector

structure and release kinetics is being developed, in order to facilitate conception of

future generations of vectors. Finally, industry-supported vectorization approach were

explored with the aim of using milk proteins alginate and chitosan for encapsulation.

Functionalization and Vectorization of Biomolecules

10

The overall objective of Workpackage 3 (WP3) was to unite the 10 local platforms carrying out biomolecule characterization, with the aim of developing a broad spectrum of tests permitting the full biological evaluation of any newly-identified compounds.

The substantial investment made to support the present project with state-of-the-art

equipment (more than €3M through CPER and 3BR project financing – in addition to the

phytotron investment previously cited), coupled with developing innovative methods for

measuring antioxidant, anti-inflammatory and anti-proliferative properties, render our

overall platform one of the most powerful within Europe. As a direct result of our efforts,

compounds obtained from the LUE IMPACT Biomolecules project or from our industry

partners can be directly and efficiently evaluated for a number of clinically- and/or com-

mercially-relevant properties. To advertise the platform, and in collaboration with the

‘Biomolecules’ group of the IAR competitivity pole, a pamphlet detailing the respective

competences of all of the participating laboratories in being prepared and furnished to

potential partner companies.

Throughout the work, a central goal has been to establish in vitro tests as alternatives

to animal experimentation. As a result, a new anti-inflammatory screening approach

has been developed, and validated via close collaboration with an industrial partner

(22 compounds tested). In addition, a new blood-brain barrier model was elaborated, which

allows characterization of the transport of both naked molecules and delivery vectors into

neurons. Finally, a new protein target for the identification of molecules with anti-inflam-

matory properties (lipolysis-stimulated lipoprotein receptor (LSR)) has been evaluated.

Functional Assessment of Biomolecules

10

The overall objective of Workpackage 3 (WP3) was to unite the 10 local platforms carrying out biomolecule characterization, with the aim of developing a broad spectrum of tests permitting the full biological evaluation of any newly-identified compounds.

The substantial investment made to support the present project with state-of-the-art

equipment (more than €3M through CPER and 3BR project financing – in addition to the

phytotron investment previously cited), coupled with developing innovative methods for

measuring antioxidant, anti-inflammatory and anti-proliferative properties, render our

overall platform one of the most powerful within Europe. As a direct result of our efforts,

compounds obtained from the LUE IMPACT Biomolecules project or from our industry

partners can be directly and efficiently evaluated for a number of clinically- and/or com-

mercially-relevant properties. To advertise the platform, and in collaboration with the

‘Biomolecules’ group of the IAR competitivity pole, a pamphlet detailing the respective

competences of all of the participating laboratories in being prepared and furnished to

potential partner companies.

Throughout the work, a central goal has been to establish in vitro tests as alternatives

to animal experimentation. As a result, a new anti-inflammatory screening approach

has been developed, and validated via close collaboration with an industrial partner

(22 compounds tested). In addition, a new blood-brain barrier model was elaborated, which

allows characterization of the transport of both naked molecules and delivery vectors into

neurons. Finally, a new protein target for the identification of molecules with anti-inflam-

matory properties (lipolysis-stimulated lipoprotein receptor (LSR)) has been evaluated.

Functional Assessment of Biomolecules

11


A new network associating academics and industry members has been created, which now provides a readily-visible access point for industry members to the combined expertise and equip-ment offered via the LUE IMPACT Biomolecules project. This network resulted in the creation of a ‘chairs’ within the LUE IMPACT Biomolecules project, but in addition provided important benefits to the industrial partners.

Industrial Chairs

Bio4solutions (Sponsorship Chair)

The endophyte project and actions related to biocontrol, biostimulation, biofertilisation

and biomolecules led, as mentioned earlier, to the founding of the “Bio4Solutions” chair

involving 4 industrial partners. This chair, which is directed by the LAE, has a budget

of € 110k/year and concerns research and teaching activities in the field of plant control

and biostimulation. The chair kick-off took place on 14 January 2020. During the first year,

several research projects were initiated with recruitment of a PhD student (funds from

the Chair and from LUE), and a second PhD student will be recruited in 2021. Regarding

the teaching activities, an 18h program (including 9h of theoretical courses and 9h of

practical courses) has been developed, and will be made available within the context of

both undergraduate/post-graduate and continuing education, beginning in 2021.

Biomolecules4bioeconomy (Partnership Chair)

The LUE IMPACT Biomolecules project comprises 19 industrial members. Via their

membership within the project, they participated in and directly co-financed the initia-

tive via specific RDI projects. In turn, they were able to develop via collaboration with the

academic members of the project, innovative technologies of direct benefit to them and

their customers. In Biomolecules 4.0, industry members will belong, the ‘Biomolecules-

4bioeconomy Chair’, a Partnership Chair which will be launched officially in November 2021.

Industrial success stories involving 6 laboratories: CITHEFOR, IMoPA, LAE, LIBio, LRGP and SIMPA

New technology for antioxidants measurements

The European Institute of Antioxidants (IEA) established a demonstration-scale sensor

for measuring antioxidant potential.

New technology for biomolecule preservation

Genialis developed a now commercialized pressurized cryogenic technology, which permits

improved preservation of biomolecules during storage. Financial support of €2M has been

obtained from private partners to further develop the technology.

Partnership for production of green extracts

Biolie licensed a patent to Canadian partners and launched a large range of natural

extracts onto the market.

Concrete industrial application

11


A new network associating academics and industry members has been created, which now provides a readily-visible access point for industry members to the combined expertise and equip-ment offered via the LUE IMPACT Biomolecules project. This network resulted in the creation of a ‘chairs’ within the LUE IMPACT Biomolecules project, but in addition provided important benefits to the industrial partners.

Industrial Chairs

Bio4solutions (Sponsorship Chair)

The endophyte project and actions related to biocontrol, biostimulation, biofertilisation

and biomolecules led, as mentioned earlier, to the founding of the “Bio4Solutions” chair

involving 4 industrial partners. This chair, which is directed by the LAE, has a budget

of € 110k/year and concerns research and teaching activities in the field of plant control

and biostimulation. The chair kick-off took place on 14 January 2020. During the first year,

several research projects were initiated with recruitment of a PhD student (funds from

the Chair and from LUE), and a second PhD student will be recruited in 2021. Regarding

the teaching activities, an 18h program (including 9h of theoretical courses and 9h of

practical courses) has been developed, and will be made available within the context of

both undergraduate/post-graduate and continuing education, beginning in 2021.

Biomolecules4bioeconomy (Partnership Chair)

The LUE IMPACT Biomolecules project comprises 19 industrial members. Via their

membership within the project, they participated in and directly co-financed the initia-

tive via specific RDI projects. In turn, they were able to develop via collaboration with the

academic members of the project, innovative technologies of direct benefit to them and

their customers. In Biomolecules 4.0, industry members will belong, the ‘Biomolecules-

4bioeconomy Chair’, a Partnership Chair which will be launched officially in November 2021.

Industrial success stories involving 6 laboratories: CITHEFOR, IMoPA, LAE, LIBio, LRGP and SIMPA

New technology for antioxidants measurements

The European Institute of Antioxidants (IEA) established a demonstration-scale sensor

for measuring antioxidant potential.

New technology for biomolecule preservation

Genialis developed a now commercialized pressurized cryogenic technology, which permits

improved preservation of biomolecules during storage. Financial support of €2M has been

obtained from private partners to further develop the technology.

Partnership for production of green extracts

Biolie licensed a patent to Canadian partners and launched a large range of natural

extracts onto the market.

Concrete industrial application

New technology for continuous CO2 extraction

The ER-Ingénierie Company developed demonstration equipment for high-efficiency

supercritical CO2 extraction

Molecule patent and production

The Metabolic Engineering Project identified potential pathways for the production of

molecules of interest to an industrial partner (Collaboration with PAT). An industrial

contract has been obtained on the basis of two target molecules, the first of which has

led to a patent application, and the second for which a functional screening method will

be developed.

Extract characterization

A research collaboration contract has been in place with Plant Advanced Technologies

(PAT) since 2018, with the aim of screening proprietary large biodiversity extract libraries

owned by PAT for new anti-inflammatory and/or anti-oxidant molecules. In this context,

functional medium/high-throughput bio-assays were developed and are complemented

by fractionation and identification expertise from PAT. As a result of these efforts, PAT

launched in 2019 a new biomolecule extracted from plants for the treatment of psoriasis

(creation of the company Temisis Therapeutics).

Stambomycin project valorization

A declaration of invention (DOI) has been made with the SATT SAYENS, on the basis of mul-

tiple novel stambomycin analogues produced by mutasynthesis (TA1). In addition, several

companies are in discussion with TA1’s partners to valorize the biomolecules produced in

the project. These include BASF, as well as Isomerase Therapeutics based in Cambridge.

Isomerase in particular is ideally positioned to facilitate future commercial application

of any promising stambomycin derivatives.

Dye biomolecules

A new collaboration with the start-up Abolis Biotechnologies, will leverage synthetic

biology approaches to create a novel source of high-value dyes for application in the

food and industries. In addition, a joint ANR LabCom application was approved (2021) to

further the work.

1212

New technology for continuous CO2 extraction

The ER-Ingénierie Company developed demonstration equipment for high-efficiency

supercritical CO2 extraction

Molecule patent and production

The Metabolic Engineering Project identified potential pathways for the production of

molecules of interest to an industrial partner (Collaboration with PAT). An industrial

contract has been obtained on the basis of two target molecules, the first of which has

led to a patent application, and the second for which a functional screening method will

be developed.

Extract characterization

A research collaboration contract has been in place with Plant Advanced Technologies

(PAT) since 2018, with the aim of screening proprietary large biodiversity extract libraries

owned by PAT for new anti-inflammatory and/or anti-oxidant molecules. In this context,

functional medium/high-throughput bio-assays were developed and are complemented

by fractionation and identification expertise from PAT. As a result of these efforts, PAT

launched in 2019 a new biomolecule extracted from plants for the treatment of psoriasis

(creation of the company Temisis Therapeutics).

Stambomycin project valorization

A declaration of invention (DOI) has been made with the SATT SAYENS, on the basis of mul-

tiple novel stambomycin analogues produced by mutasynthesis (TA1). In addition, several

companies are in discussion with TA1’s partners to valorize the biomolecules produced in

the project. These include BASF, as well as Isomerase Therapeutics based in Cambridge.

Isomerase in particular is ideally positioned to facilitate future commercial application

of any promising stambomycin derivatives.

Dye biomolecules

A new collaboration with the start-up Abolis Biotechnologies, will leverage synthetic

biology approaches to create a novel source of high-value dyes for application in the

food and industries. In addition, a joint ANR LabCom application was approved (2021) to

further the work.

12

13


Research contract with the SEPTEOS® Company & PhD CIFRE grant

CIFRE PhD and research contracts have been obtained to study the antifungal properties

of a formulation created by the SEPTEOS company using natural substances (essential oils,

in particular). SIMPA, one of the laboratories of IMPACT Biomolecules has a collection of

fungi that is used to determine the spectrum of activity of this mixture towards the main

genera and/or species of filamentous fungi underlying human pathologies. Furthermore,

this project aims to establish the mode of action of this product.

Labcom with Avril group with LRGP

The aim of AVALON (joint research project with AVRIL group) was to explore, develop and

scale up protein isolation pathways from sunflower and rapeseed meals. For this, mode-

ling was carried out on the impact of solid/liquid proteins extraction conditions, as well

as multiobjective optimization methodologies (including protein quality criteria). In this

framework, the joint Labcom aimed at the valorization of a liquid side-product generated

by the protein purification process. It consisted in capturing phenolic compounds from

the side product, and assessing their antioxidant and anti-inflammatory activities.

Pre-maturation grant awarded by SATT SAYENS.

The prematuration project was initially designed to focus on non-alcoholic fatty liver

disease (NAFLD). The expertise of the partners involved in the IMPACT project allowed a

broader characterization of the potential anti-NAFLD effects of Polylip1, thus increasing

chances of identifying the therapeutic potential of this compound.

Contacts with the SPIRAL Company within the context of the BCR project

The SPIRAL company has created a preparation rich in polyphenols extracted from various

plants. This preparation, coupled with omega 3 fatty acids and vitamins, was administe-

red to healthy volunteers involved in a strict physical inactivity program that lasted for

2 months. The task was to determine whether this preparation makes it possible to com-

bat osteoporosis induced by physical inactivity and to assess the effects on the immune

system. This research was conducted in the context of an EU project.

13


Research contract with the SEPTEOS® Company & PhD CIFRE grant

CIFRE PhD and research contracts have been obtained to study the antifungal properties

of a formulation created by the SEPTEOS company using natural substances (essential oils,

in particular). SIMPA, one of the laboratories of IMPACT Biomolecules has a collection of

fungi that is used to determine the spectrum of activity of this mixture towards the main

genera and/or species of filamentous fungi underlying human pathologies. Furthermore,

this project aims to establish the mode of action of this product.

Labcom with Avril group with LRGP

The aim of AVALON (joint research project with AVRIL group) was to explore, develop and

scale up protein isolation pathways from sunflower and rapeseed meals. For this, mode-

ling was carried out on the impact of solid/liquid proteins extraction conditions, as well

as multiobjective optimization methodologies (including protein quality criteria). In this

framework, the joint Labcom aimed at the valorization of a liquid side-product generated

by the protein purification process. It consisted in capturing phenolic compounds from

the side product, and assessing their antioxidant and anti-inflammatory activities.

Pre-maturation grant awarded by SATT SAYENS.

The prematuration project was initially designed to focus on non-alcoholic fatty liver

disease (NAFLD). The expertise of the partners involved in the IMPACT project allowed a

broader characterization of the potential anti-NAFLD effects of Polylip1, thus increasing

chances of identifying the therapeutic potential of this compound.

Contacts with the SPIRAL Company within the context of the BCR project

The SPIRAL company has created a preparation rich in polyphenols extracted from various

plants. This preparation, coupled with omega 3 fatty acids and vitamins, was administe-

red to healthy volunteers involved in a strict physical inactivity program that lasted for

2 months. The task was to determine whether this preparation makes it possible to com-

bat osteoporosis induced by physical inactivity and to assess the effects on the immune

system. This research was conducted in the context of an EU project.

14

Cobalamin project with Cobalz Limited

Thiolatocobalamins such as N-acetyl-L-cysteinylcobalamin and glutathionylcobalamin

directly target the cobalamin metabolism pathway, producing antioxidant effects. It has

been previously shown that these compounds inhibit intracellular peroxide production,

maintain intracellular glutathione levels, and prevent apoptotic and necrotic cell death.

Such agents have superior efficacy compared with other forms of cobalamin, alternative

cobalamins in combination with N-acetyl-L-cysteine (NAC) or glutathione (GSH), and NAC

or GSH alone.

Metal chelating peptides

The BASF company is interested in metal chelating peptides for skin and hair applications.

BASF provided a letter of support for a successful ANR JCJC application during the 2020

ANR call. The program is presently running.

Biomolecules encapsulation

The Cookal Company is currently funding a CIFRE PhD thesis (2021−2024) on the reticu-

lation of gels for the encapsulation of active compounds.

Active proteins valorisation

The international group ARLA food is currently funding a 3-year contract to develop new

peptides for nutritional applications

Milk Powder stabilisation

The Nestlé international group studies the structures of food powders, in order to model

and control molecular diffusion in particles. At present, this project is being extended to

the extraction and stabilization of active biomolecules.

3BR – A major Regional project:

The LUE IMPACT Biomolecules project, along with 13 other regional laboratories (located

in Reims, Troyes, Strasbourg, Colmar, Metz and Nancy) was awarded a further €2.5M

(via the 2019 FRCR call) to valorize biomolecules from abundant regional natural resources

(hemp, hop and rapeseed meal), in close collaboration with agriculturalists and industrial

end-users. The project (called ‘Biomolecules and Biomaterials for the Regional Bioeconomy

– 3BR’ 2020−2022) aims to extract, functionalize and vectorize component biomolecules.

These efforts will confer significant added value to what are classically ‘waste’ products,

and make the Biomolecules IMPACT project a European model for new methods to advance

towards a circular, ‘zero waste’ economy.

1414

Cobalamin project with Cobalz Limited

Thiolatocobalamins such as N-acetyl-L-cysteinylcobalamin and glutathionylcobalamin

directly target the cobalamin metabolism pathway, producing antioxidant effects. It has

been previously shown that these compounds inhibit intracellular peroxide production,

maintain intracellular glutathione levels, and prevent apoptotic and necrotic cell death.

Such agents have superior efficacy compared with other forms of cobalamin, alternative

cobalamins in combination with N-acetyl-L-cysteine (NAC) or glutathione (GSH), and NAC

or GSH alone.

Metal chelating peptides

The BASF company is interested in metal chelating peptides for skin and hair applications.

BASF provided a letter of support for a successful ANR JCJC application during the 2020

ANR call. The program is presently running.

Biomolecules encapsulation

The Cookal Company is currently funding a CIFRE PhD thesis (2021−2024) on the reticu-

lation of gels for the encapsulation of active compounds.

Active proteins valorisation

The international group ARLA food is currently funding a 3-year contract to develop new

peptides for nutritional applications

Milk Powder stabilisation

The Nestlé international group studies the structures of food powders, in order to model

and control molecular diffusion in particles. At present, this project is being extended to

the extraction and stabilization of active biomolecules.

3BR – A major Regional project:

The LUE IMPACT Biomolecules project, along with 13 other regional laboratories (located

in Reims, Troyes, Strasbourg, Colmar, Metz and Nancy) was awarded a further €2.5M

(via the 2019 FRCR call) to valorize biomolecules from abundant regional natural resources

(hemp, hop and rapeseed meal), in close collaboration with agriculturalists and industrial

end-users. The project (called ‘Biomolecules and Biomaterials for the Regional Bioeconomy

– 3BR’ 2020−2022) aims to extract, functionalize and vectorize component biomolecules.

These efforts will confer significant added value to what are classically ‘waste’ products,

and make the Biomolecules IMPACT project a European model for new methods to advance

towards a circular, ‘zero waste’ economy.

1515


15

16

European collaborationsOne of the major objectives of the LUE IMPACT Biomolecules project is to create a research

nexus centered in the Lorraine/Grand Est, but which is recognized at the European level

as one of the leaders in innovation of biomolecules sourced from microbes, as well as

from regional agricultural and forestry resources.

Networking and structuring of the researchThe total value of the LUE IMPACT Biomolecules project was €9M: €2.5M from LUE, €3.5M from industrial members and other external funding, and €3M from the FRCR project. Moreover, CPER funds contributed as much as €4M worth of equipment to the LUE IMPACT Biomolecules platforms.

Structuring of Biomolecules labs New collaborations between teams and laboratories within NancyNew collaborations were established during the LUE IMPACT Biomolecules project between

LAE and L2CM; LRGP and LERMAB; LCPM and LIBio; LCPM and LERMAB; IMoPA, CITHEFOR

and PAT; URAFPA and IMoPA; CALBINOTOX, CITHEFOR, LRGP, LCPM, CRAN and MEDyC;

DCAC, CITHEFOR and ACBS; IMoPA, DCAC and LORIA; IMoPA, DYNAMIC, LRGP, CRM2 and

the PASM platform; SIMPA and LRGP; SIMPA and L2CM.

Enlargement of regional collaborationsAs noted early, the 3BR project involved 13 additional laboratories within the Grand Est

region.

Organisation of international conferences• Organisation of an autumn school and and international conference in Palma di Majorca

Spain in October 2019

• Organisation of a European conference in Nancy Palais des congrès France

in November 202116

European collaborationsOne of the major objectives of the LUE IMPACT Biomolecules project is to create a research

nexus centered in the Lorraine/Grand Est, but which is recognized at the European level

as one of the leaders in innovation of biomolecules sourced from microbes, as well as

from regional agricultural and forestry resources.

Networking and structuring of the researchThe total value of the LUE IMPACT Biomolecules project was €9M: €2.5M from LUE, €3.5M from industrial members and other external funding, and €3M from the FRCR project. Moreover, CPER funds contributed as much as €4M worth of equipment to the LUE IMPACT Biomolecules platforms.

Structuring of Biomolecules labs New collaborations between teams and laboratories within NancyNew collaborations were established during the LUE IMPACT Biomolecules project between

LAE and L2CM; LRGP and LERMAB; LCPM and LIBio; LCPM and LERMAB; IMoPA, CITHEFOR

and PAT; URAFPA and IMoPA; CALBINOTOX, CITHEFOR, LRGP, LCPM, CRAN and MEDyC;

DCAC, CITHEFOR and ACBS; IMoPA, DCAC and LORIA; IMoPA, DYNAMIC, LRGP, CRM2 and

the PASM platform; SIMPA and LRGP; SIMPA and L2CM.

Enlargement of regional collaborationsAs noted early, the 3BR project involved 13 additional laboratories within the Grand Est

region.

Organisation of international conferences• Organisation of an autumn school and and international conference in Palma di Majorca

Spain in October 2019

• Organisation of a European conference in Nancy Palais des congrès France

in November 2021

17


Fig. 1. European collaboration partners currently involved in the LUE IMPACT Biomolecules project.The IMPACT Biomolecules consortium allowed for forging links with 33 European partners from 12 different countries. It also allowed each member to join a multidisciplinary network aiming at full valorization of bioresources, furthering the goal of attaining a truly circular/zero waste bioeconomy in Europe. The consortium led to strong networking with European academic and industry experts in the fields of characterization/vectorization/functionalization/synthesis of biomolecules, resulting in multiple new research projects. The result of these projects are publications in high impact journals, international conference presentations, novel IP, and most importantly, multi-national alignment of scientific strategies related to biomolecules and the bioeconomy, which will be exploited in the context of Biomolecules 4.0.

17


Fig. 1. European collaboration partners currently involved in the LUE IMPACT Biomolecules project.The IMPACT Biomolecules consortium allowed for forging links with 33 European partners from 12 different countries. It also allowed each member to join a multidisciplinary network aiming at full valorization of bioresources, furthering the goal of attaining a truly circular/zero waste bioeconomy in Europe. The consortium led to strong networking with European academic and industry experts in the fields of characterization/vectorization/functionalization/synthesis of biomolecules, resulting in multiple new research projects. The result of these projects are publications in high impact journals, international conference presentations, novel IP, and most importantly, multi-national alignment of scientific strategies related to biomolecules and the bioeconomy, which will be exploited in the context of Biomolecules 4.0.

18

Project indicators Indicator title Description Measurement unit

Development of scientific excellence Publication strength % of publications from the academic partners

in Q1 journals

PhDs PhDs funded by academic and industrial partners Number of PhD students (of which CIFRE)

Post-Docs Post-docs working in both the academic and industrial partners Post doc numbers

Public-private collaborations Research interactions Number of collaborative contracts obtained

Technology transfer

Patent deposits Number of deposits (to INPI, OEB, USPTO...)

Projects supported by the SATT SAYENS Number of projects

Economic development Creation of Start-Ups Number of Start-Ups created

Development of international academic partnerships

Funded research actions with « Grande Région » partners (Sarre, Lor, Lux, Wallonie, Rhénanie) Number of associated « Grande Région » partners

Curriculum development Creation of a Master’s program (from 2023) centered on Bio-molecules and the Bioeconomy Number of Master’s created

Inaugural conference

The aim was to bring together the partner LUE labs and iden-tified private partners in order to inform them of the initiative, to describe the current state-of-the-art, and to solicit their confinancement of specific future aspects

International conferenceOrganize an international conference with UL partners, world-leading economy companies and start-ups in the field of pharmacy, cosmetics, food and agrochemistry

Autumn school Organize an autumn school for PhD students about Biomole-cules valorization

Scientific newsletter Production of a monthly letter for partners with latest international scientific and economic news

International collaborations Organize a meeting with KYUTECH representatives

National Recognition Participation in the Bioeconomy prize 2019

18

Project indicators Indicator title Description Measurement unit

Development of scientific excellence Publication strength % of publications from the academic partners

in Q1 journals

PhDs PhDs funded by academic and industrial partners Number of PhD students (of which CIFRE)

Post-Docs Post-docs working in both the academic and industrial partners Post doc numbers

Public-private collaborations Research interactions Number of collaborative contracts obtained

Technology transfer

Patent deposits Number of deposits (to INPI, OEB, USPTO...)

Projects supported by the SATT SAYENS Number of projects

Economic development Creation of Start-Ups Number of Start-Ups created

Development of international academic partnerships

Funded research actions with « Grande Région » partners (Sarre, Lor, Lux, Wallonie, Rhénanie) Number of associated « Grande Région » partners

Curriculum development Creation of a Master’s program (from 2023) centered on Bio-molecules and the Bioeconomy Number of Master’s created

Inaugural conference

The aim was to bring together the partner LUE labs and iden-tified private partners in order to inform them of the initiative, to describe the current state-of-the-art, and to solicit their confinancement of specific future aspects

International conferenceOrganize an international conference with UL partners, world-leading economy companies and start-ups in the field of pharmacy, cosmetics, food and agrochemistry

Autumn school Organize an autumn school for PhD students about Biomole-cules valorization

Scientific newsletter Production of a monthly letter for partners with latest international scientific and economic news

International collaborations Organize a meeting with KYUTECH representatives

National Recognition Participation in the Bioeconomy prize 2019

19


Target at 2.5 yrs Today Comments

50% 80% Our secondary objective is to publish 3 opinion papers on the theme of valorizing biomolecules (open access)

6(0) 21(3)

50% financed by the LUE and 50% by private companies

8 29

5 35 Increasing participation from the private sector. Creation of the 3B Network

3 16 by industry partners Additional patents by industrial members + SATT

2 5 Maturation projects

2-31 new and further development of 5 existing start-ups

The project financially supports all 5 partner start-ups and participates in their scientific and research development

5 13

Planned submission of a ‘Bioengineering Valley’ Interreg project. This project was transformed into a partnership chair ‘Biomolecules4 Bioeconomy’ (B4B) encompassing private and public partners. These collaborations were established via the FRCR project funded by the Region Grand Est (the project is called ‘Biomolecules and Biomaterials for the Regional Bioeconomy (3BR))

0 1 Creation of a Master mention in Biomolecules and the Bioeconomy in collaboration with URCA

1 1« Biomolécules du futur : de la découverte aux applications »Researchers, competitivity clusters (pôles de compétititivé), and companies (such as BASF, Nestle, Danone, SEPPIC, Avril and LVMH) were present and BtoB were organized.

1 2

‘Biomolecules: Research & Development, Markets and Acceptability’ which took place from Wednesday, 23 October to Friday, 25 October 25, in Palma de Mallorca, Spain‘Metal-Binding Peptides: Methodologies and Applications’ MBP 2020 which was to take place in Nancy, France from 7−10 July 2020. (Delayed due to Covid-19 until 5−8 July 2021)

0 1

A 3-day ‘autumn school’ was organized during which the following key themes were addressed:• Identification and generation of new biomolecules of interest• Functionalization, vectorization, and integration of biomolecules into complex matrices• Characterization of biological activities and applications

0 18 Support from the partenarial department (DEPAS) of the UL and from a PhD student

0 1 A meeting was organized for late 2020 but had to be postponed due to Covid-19

0 Ranked Ranked in the top five of the national projects

19


Target at 2.5 yrs Today Comments

50% 80% Our secondary objective is to publish 3 opinion papers on the theme of valorizing biomolecules (open access)

6(0) 21(3)

50% financed by the LUE and 50% by private companies

8 29

5 35 Increasing participation from the private sector. Creation of the 3B Network

3 16 by industry partners Additional patents by industrial members + SATT

2 5 Maturation projects

2-31 new and further development of 5 existing start-ups

The project financially supports all 5 partner start-ups and participates in their scientific and research development

5 13

Planned submission of a ‘Bioengineering Valley’ Interreg project. This project was transformed into a partnership chair ‘Biomolecules4 Bioeconomy’ (B4B) encompassing private and public partners. These collaborations were established via the FRCR project funded by the Region Grand Est (the project is called ‘Biomolecules and Biomaterials for the Regional Bioeconomy (3BR))

0 1 Creation of a Master mention in Biomolecules and the Bioeconomy in collaboration with URCA

1 1« Biomolécules du futur : de la découverte aux applications »Researchers, competitivity clusters (pôles de compétititivé), and companies (such as BASF, Nestle, Danone, SEPPIC, Avril and LVMH) were present and BtoB were organized.

1 2

‘Biomolecules: Research & Development, Markets and Acceptability’ which took place from Wednesday, 23 October to Friday, 25 October 25, in Palma de Mallorca, Spain‘Metal-Binding Peptides: Methodologies and Applications’ MBP 2020 which was to take place in Nancy, France from 7−10 July 2020. (Delayed due to Covid-19 until 5−8 July 2021)

0 1

A 3-day ‘autumn school’ was organized during which the following key themes were addressed:• Identification and generation of new biomolecules of interest• Functionalization, vectorization, and integration of biomolecules into complex matrices• Characterization of biological activities and applications

0 18 Support from the partenarial department (DEPAS) of the UL and from a PhD student

0 1 A meeting was organized for late 2020 but had to be postponed due to Covid-19

0 Ranked Ranked in the top five of the national projects

Key project indicatorsMore than 200 research outputs resulted from the project. These incude:

For details, see Annexe 2

Publications Book chapters

Oral presentations

Database

Posters

Conference organisation

Patents

Publications in peer-reviewed journals whose topic is related to the project

51 72 4

42 37 16

1 3

2020

Key project indicatorsMore than 200 research outputs resulted from the project. These incude:

For details, see Annexe 2

Publications Book chapters

Oral presentations

Database

Posters

Conference organisation

Patents

Publications in peer-reviewed journals whose topic is related to the project

51 72 4

42 37 16

1 3

20

21


Hiring of PhDs Post-doctoral researchers Others

LUE IMPACT Biomolecules Research activities involved 8 post-docs, 8 PhD and 10 MSc students, and approximately 20 short-term student interns.

Post-doctoral fellowship LUE Biomolecule – Région Grand Est. Ryosuke MUNAKATA (LAE – April 2019 – March 2020) and Ludivine HOCQ (April 2020–September 2020) : Isola-tion of a di-prenyltransferase gene from Artemisia capillaris and redesign of bioactive prenylated polyphenols in yeast

Post-doctoral fellowship (LIBio – LCPM – LRGP – URAFPA). Amina BEN MIHOUB (March 2018–August 2020) : Active targeted nanodelivery as effective prevention and risk reduction of Alzheimer’s disease

Post-doctoral fellowship (LCPM – LERMAB). Ali KHALIL (April 2019–September 2020) : Vectorization of antioxidant gallic acid via bioactive polymer materials

Post-doctoral fellowship (IMOPA – CITHEFOR – PAT). Stefania Enza SESTITO (July 2018–May 2020) : Simple targeted cell-based assay for the screening of natural extracts libraries with anti-inflammatory properties

Post-doctoral fellowship (CIBLSR – IMoPA – URAFPA). Hela SAFI (April 2019–September 2020) : LSR as a new potential target: Purification and characterization of its recombi-nant and circulating forms

Post-doctoral fellowship (CALBINOTOX – CITHEFOR). Venus SOSA IGLESIAS (April 2019 – January 2021) : Blood-brain Barrier (BBB) – Passage of functionalized mo-lecules for antitumor activity

Post-doctoral fellowship (DynAMic – IMoPA). Yaouba SOUAIBOU (April 2019–September 2020) : Generation of new polyketide analogues by synthetic niology approaches : towards purification and biological evaluation of newly synthesized compounds

Post-doctoral fellowship (Gemm_Est). Sébastien RIBEIRO (November 2019–December 2021) : The Gemm_Est project : tapping reintroduction in the eastern regions of France, from oleoresin to genes

Post-doctoral fellowship (LCPM – LRGP – L2CM). Gizella CSIRE (March 2019–November 2020) : Iron (III)-chelating model peptides as bioinspired antioxidants

Post DocsWP1

WP2

WP3

TA1

TA2

TA3

21


21


Hiring of PhDs Post-doctoral researchers Others

LUE IMPACT Biomolecules Research activities involved 8 post-docs, 8 PhD and 10 MSc students, and approximately 20 short-term student interns.

Post-doctoral fellowship LUE Biomolecule – Région Grand Est. Ryosuke MUNAKATA (LAE – April 2019 – March 2020) and Ludivine HOCQ (April 2020–September 2020) : Isola-tion of a di-prenyltransferase gene from Artemisia capillaris and redesign of bioactive prenylated polyphenols in yeast

Post-doctoral fellowship (LIBio – LCPM – LRGP – URAFPA). Amina BEN MIHOUB (March 2018–August 2020) : Active targeted nanodelivery as effective prevention and risk reduction of Alzheimer’s disease

Post-doctoral fellowship (LCPM – LERMAB). Ali KHALIL (April 2019–September 2020) : Vectorization of antioxidant gallic acid via bioactive polymer materials

Post-doctoral fellowship (IMOPA – CITHEFOR – PAT). Stefania Enza SESTITO (July 2018–May 2020) : Simple targeted cell-based assay for the screening of natural extracts libraries with anti-inflammatory properties

Post-doctoral fellowship (CIBLSR – IMoPA – URAFPA). Hela SAFI (April 2019–September 2020) : LSR as a new potential target: Purification and characterization of its recombi-nant and circulating forms

Post-doctoral fellowship (CALBINOTOX – CITHEFOR). Venus SOSA IGLESIAS (April 2019 – January 2021) : Blood-brain Barrier (BBB) – Passage of functionalized mo-lecules for antitumor activity

Post-doctoral fellowship (DynAMic – IMoPA). Yaouba SOUAIBOU (April 2019–September 2020) : Generation of new polyketide analogues by synthetic niology approaches : towards purification and biological evaluation of newly synthesized compounds

Post-doctoral fellowship (Gemm_Est). Sébastien RIBEIRO (November 2019–December 2021) : The Gemm_Est project : tapping reintroduction in the eastern regions of France, from oleoresin to genes

Post-doctoral fellowship (LCPM – LRGP – L2CM). Gizella CSIRE (March 2019–November 2020) : Iron (III)-chelating model peptides as bioinspired antioxidants

Post DocsWP1

WP2

WP3

TA1

TA2

TA3

22

Doctoral fellowship (DynAMic – IAM). Matthieu NICAULT (September 2017–November 2020) : Deciphering and exploiting bacteria-fungi dialogues to identify new bioactive metabolites

Doctoral fellowship (LAE – L2CM). Yuka SAIDA-MUNAKATA (September 2018–December 2021) : Study of plant-bacteria interaction to identify endophytes involved in the synthesis of active molecules and usable for biosolutions.

Doctoral fellowship (LERMAB – LRGP). Georges EID (November 2018–October 2021) : New lipophilic and amphiphilic derivatives by functionalization of amino-acid and xylo-sourced phenolic compounds

Doctoral fellowship (LIBio – LCPM). Jiaqi WENG (November 2018–October 2021) : Encapsulation in chitosan structured particles for controlled release of biomolecules

Doctoral fellowship (DynAMic – IMoPA). Li SU (December 2017–May 2021) : Generation of analogues of stambomycins by genetic engineering and allied approaches

Doctoral fellowship (LRGP – SIMPA). Thi Tuong LE (November 2017–June 2021) : Purification and biomedical valorization of model phenolic fractions

Doctoral fellowship – CIFRE grant (SIMPA). Maurine D’AGOSTINO (October 2018–September 2021) : Characterization of a compound of plant origin on septal filamentous fungi and more particularly Fusarium solanii

Doctoral fellowship (LRGP – CITHEFOR). Sarah EL HAJJ (September 2018–September 2021) : SPR screening of metal-chelating peptides for their antioxidant properties

PhD (LIBIO-LRGP). Cédric PARIS (September 2017–Avril 2021) : Development of new ana-lytical approaches for the screening of metal-chelating peptides

MESR Doctoral fellowship (LRGP) : Rachel IRANKUNDA (October 2020–October 2023) : Labeled Impact Biomolecules. Metal-chelating peptides and chromatography : from peptides separation to metals separation.

PhDsWP1

WP2

TA1

TA2

TA3

2222

Doctoral fellowship (DynAMic – IAM). Matthieu NICAULT (September 2017–November 2020) : Deciphering and exploiting bacteria-fungi dialogues to identify new bioactive metabolites

Doctoral fellowship (LAE – L2CM). Yuka SAIDA-MUNAKATA (September 2018–December 2021) : Study of plant-bacteria interaction to identify endophytes involved in the synthesis of active molecules and usable for biosolutions.

Doctoral fellowship (LERMAB – LRGP). Georges EID (November 2018–October 2021) : New lipophilic and amphiphilic derivatives by functionalization of amino-acid and xylo-sourced phenolic compounds

Doctoral fellowship (LIBio – LCPM). Jiaqi WENG (November 2018–October 2021) : Encapsulation in chitosan structured particles for controlled release of biomolecules

Doctoral fellowship (DynAMic – IMoPA). Li SU (December 2017–May 2021) : Generation of analogues of stambomycins by genetic engineering and allied approaches

Doctoral fellowship (LRGP – SIMPA). Thi Tuong LE (November 2017–June 2021) : Purification and biomedical valorization of model phenolic fractions

Doctoral fellowship – CIFRE grant (SIMPA). Maurine D’AGOSTINO (October 2018–September 2021) : Characterization of a compound of plant origin on septal filamentous fungi and more particularly Fusarium solanii

Doctoral fellowship (LRGP – CITHEFOR). Sarah EL HAJJ (September 2018–September 2021) : SPR screening of metal-chelating peptides for their antioxidant properties

PhD (LIBIO-LRGP). Cédric PARIS (September 2017–Avril 2021) : Development of new ana-lytical approaches for the screening of metal-chelating peptides

MESR Doctoral fellowship (LRGP) : Rachel IRANKUNDA (October 2020–October 2023) : Labeled Impact Biomolecules. Metal-chelating peptides and chromatography : from peptides separation to metals separation.

PhDsWP1

WP2

TA1

TA2

TA3


Master 1 internship (IAM – DynAMic). Isabelle CANIHAC (May 2018–August 2018) : Recherche d’activités antimicrobiennes et herbicides chez des bactéries du microbiome de la truffe

Master internship (LRGP – L2CM). Alexandre DERET (April 2019–July 2019) : Production and immobilization of aminoacylases from Streptomyces ambofaciens

Master internship (LIBio – LCPM – LRGP – URAFPA). Maria Camila JIMENEZ (February 2019– June 2019) : Vectorisation of active molecules by nanoliposomes: evaluation of neuroprotective effects

Master internship (LIBio – LCPM). Manon ALLIO (February 2019–July 2019) : Chemical modification of dextran and chitosan

Master 2 internship (L2CM – SIMPA). Safa DADI (February 2019–July 2019) : Screening extracts from fungal endophytes to identify new molecules with anti-inflammatory activity

Master 2 internship (L2CM). Sihem BOUAZZI (February 2021–July 2021) :Purification of extracts from fungal endophytes to identify new molecules with anti-inflammatory activity

Master internship (LRGP). Armel D’Autriche (August 2018–February 2019) : Purification and biomedical valorization of model phenolic fractions

License internship (URAFPA – LRGP). Léa VOLLES (February 2018–May 2018) : Identification of therapeutic strategies against hepatic steatosis

Master 2 internship (LERMAB). Jakub KERNAL (September 2019–February 2020) : Prenyflav: PRENYlated FLAVonoids

Master internship (L2CM – LCPM - LRGP). Lynda SAIDI (February 2018 – July 2018) : ImplicIT: Interactions between peptides and metal ions: understanding and innovation

MScWP1

WP2

TA2

TA3

2323

FINAL REPORT LUE IMPACT Biomolecules projectFINAL REPORT LUE IMPACT Biomolecules project

Master 1 internship (IAM – DynAMic). Isabelle CANIHAC (May 2018–August 2018) : Recherche d’activités antimicrobiennes et herbicides chez des bactéries du microbiome de la truffe

Master internship (LRGP – L2CM). Alexandre DERET (April 2019–July 2019) : Production and immobilization of aminoacylases from Streptomyces ambofaciens

Master internship (LIBio – LCPM – LRGP – URAFPA). Maria Camila JIMENEZ (February 2019– June 2019) : Vectorisation of active molecules by nanoliposomes: evaluation of neuroprotective effects

Master internship (LIBio – LCPM). Manon ALLIO (February 2019–July 2019) : Chemical modification of dextran and chitosan

Master 2 internship (L2CM – SIMPA). Safa DADI (February 2019–July 2019) : Screening extracts from fungal endophytes to identify new molecules with anti-inflammatory activity

Master 2 internship (L2CM). Sihem BOUAZZI (February 2021–July 2021) :Purification of extracts from fungal endophytes to identify new molecules with anti-inflammatory activity

Master internship (LRGP). Armel D’Autriche (August 2018–February 2019) : Purification and biomedical valorization of model phenolic fractions

License internship (URAFPA – LRGP). Léa VOLLES (February 2018–May 2018) : Identification of therapeutic strategies against hepatic steatosis

Master 2 internship (LERMAB). Jakub KERNAL (September 2019–February 2020) : Prenyflav: PRENYlated FLAVonoids

Master internship (L2CM – LCPM - LRGP). Lynda SAIDI (February 2018 – July 2018) : ImplicIT: Interactions between peptides and metal ions: understanding and innovation

MScWP1

WP2

TA2

TA3

23

24

Annexe 1 Promoting BiomoleculesWithin the framework of LUE we have developed a communication tool (Promoting Biomolecules - ProBio) for the general public to

explain the scientific process implemented in the Biomolecules research program. This tool is divided into 3 main parts: a film, a comic

book and a photo exhibition. The comic strip has been translated into 3 languages: French, German and English.

24

Annexe 1 Promoting BiomoleculesWithin the framework of LUE we have developed a communication tool (Promoting Biomolecules - ProBio) for the general public to

explain the scientific process implemented in the Biomolecules research program. This tool is divided into 3 main parts: a film, a comic

book and a photo exhibition. The comic strip has been translated into 3 languages: French, German and English.

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Annexe 2List of publications, oral communications, patents etc.

Work-Package 1 : Biotic interactions and biosynthetic pathways

Publications

Munakata R., Olry A., Takemura T., Tatsumi K., Ichino T., Kageyama J., T. Kurata, Sakamoto T., Nakayasu M., Mizutani M., Jacob F., Yamamoto H., Koeduka T., Moriyoshi E., Grosjean J., Krieger C., Sugiyama A., Bourgaud F., Hehn A., and Yazaki K. Parallel evolution of UbiA superfamily proteins into aromatic O-prenyltransferases in plants. (2021) PNAS (Sous presse)

Nicault M., Tidjani A-R., Gauthier A., Dumarcay S., Gelhaye E., Bontemps C., Leblond P. Elicitation of antimicrobially active compounds by Strep-tomyces-Fungus co-cultures. (2021) Microorganisms 9(1), 178 (DOI 10.3390/microorganisms9010178)

Spina R., Saliba S., Dupire F., Ptak A., Hehn A., Piutti S., Poinsignon S. , Leclerc S., Bouguet-Bonnet S. , Laurain-Mattar D. Molecular Identification of Endophytic Bacteria in Leucojum aestivum In Vitro Culture, NMR-Based Metabolomics Study and LC-MS Analysis Leading to Potential Amaryllidaceae Alkaloid Production. (2021) International Journal of Molecular Sciences, 22 (4), pp.1773. (DOI: 10.3390/ijms22041773).

Munakata Y., Gavira C., Genestier J., Bourgaud F., Hehn A., Slezack-Des-chaumes S. Composition and functional comparison of vetiver root endo-phytic microbiota originating from different geographic locations that show antagonistic activity towards Fusarium graminearum (2021) Microbiological Research 243, 126650 (doi.org/10.1016/j.micres.2020.126650)

Miguel S., Legrand G., Duriot L., Delporte M., Menin B., Michel C., Olry A., Chataigné G., Salwinski A., Bygdell J., Vercaigne D., Wingsle G., Hilbert J.L., Bourgaud F., Hehn A., Gagneul D.. Recombinant GDSL lipase-like protein from Ipomoea batatas expressed in Pichia pastoris for an efficient biosynthesis of 3,5-dicaffeoylquinic acid, a promising anti-viral and anti-inflammatory candidate. (2020) Communications biology, 3, 673 (DOI 10.1038/s42003-020-01387-1)

Nicault M., Tidjani A-R., Gauthier A., Dumarcay S., Gelhaye E., Bontemps C., Leblond P. (2020) Mining the biosynthetic potential for specialized meta-bolism of a Streptomyces soil community. MBPI 9(5), 271 (DOI: 10.3390/antibiotics9050271)

Khiralla A., R. Spina, Varbanov V., Philippot S., Lemiere P., Slezack-Des-chaumes S., André P., Mohamed I., Yagi S., Laurain-Mattar D. “Evaluation of Antiviral, Antibacterial and Antiproliferative Activities of the Endophytic Fungus Curvularia papendorfii, and Isolation of a New Polyhydroxyacid (2020), Microorganisms, 8 (9), pp.1353 (doi: 10.3390/microorganisms8091353)

Hemmati N., Azizi S., Spina R., Dupire F., Arouei H. , Saeedi M., Laurain-Mat-tar,D. Accumulation of ajmalicine and vinblastine in cell cultures is enhanced by endophytic fungi of Catharanthus roseus cv. Icy Pink (2020), Ind. Crops Prod., Elsevier, 158, pp.112776 (doi: 10.1016/j.indcrop.2020.112776)

Limones-Mendez M., Dugrand-Judek A., Villard C., Coqueret V., Froelicher Y., Bourgaud F., Olry A., Hehn A. Convergent evolution leading to the appearance of furanocoumarins in citrus plants. (2020) Plant science, 92. (doi. 10.1016/j.plantsci.2019.110392

Munakata R., Kitajima S., Nuttens A., Tatsumi K., Takemura T., Ichino T., Galati G., Vautrin S., Berges H., Grosjean J., Bourgaud F., Sugiyama A., Hehn A., Yazaki K.. Convergent evolution of the UbiA superfamily underlies inde-pendent acquisitions of furanocoumarins in plants. (2019) New Phytologist. 225: pp 2166–2182 (doi: 10.1111/nph.16277)

Munakata R., Takemura T., Tatsumi K., Moriyoshi E., Yanagihara K., Sugiyama A., Suzuki H., Seki H., Muranaka T., Kawano N., Yoshimatsu K., Kawahara N., Yamaura T., Grosjean J., Bourgaud F., Hehn A., Yazaki K. Isolation of Artemi-sia capillaris membrane-bound di-prenyltransferase for phenylpropanoids and redesign of artepillin C in yeast. (2019) Communications Biology. 2, p 384. (doi: 10.1038/s42003-019-0630-0

Galati G., Gandin A., Jolivet Y., Larbat R., Hehn A. Untargeted metabolomics approach reveals diverse responses of Pastinaca sativa to ozone and woun-ding stresses. (2019) Metabolites, 9, p153. (doi: 10.3390/metabo9070153.)

Villard C., Larbat R., Munakata R., Hehn A. Defence mechanisms of Ficus: pyramiding strategies to cope with stressing environments. (2019) Planta. 249(3) pp617-633 (doi: 10.1007/s00425-019-03098-2

Tarakemeh A., Azizi M., Rowshan V., Salehi H., Spina R., Dupire F., Arouie H., Laurain-Mattar D.. Screening of Amaryllidaceae alkaloids in bulbs and tissue cultures of Narcissus papyraceus and four varieties of N. tazetta. (2019) Journal of Pharmaceutical and Biomedical Analysis 172, pp.230-237. (doi: 10.1016/j.jpba.2019.04.043)

Publications in peer-reviewed journals whose topic is related to WP1:Roumani M., Duval R., Ropars A., Risler A., Robin C, Larbat R. Phenolamides : Plant specialized metabolites with a wide range of promising pharmacological and health-promoting interests (2020) Biomedicine and Pharmacotherapy, 131: 110762 (doi.org/10.1016/j.biopha.2020.110762)

Nicault M., Tidjani A-R., Gelhaye E., Bontemps C., Leblond P. Genome se-quence of 5 Streptomyces isolates from a community at microscale. (2020) MRA Volume 9 Issue 23 (DOI : 10.1128/MRA.00428-20)

Ptak A., Simlat M., Morańska E., Skrzypek E., Warchoł M., Tarakemeh A., Laurain-Mattar D. Exogenous melatonin stimulated Amaryllidaceae alkaloid biosynthesis in in vitro cultures of Leucojum aestivum L. (2019) Industrial Crops and Products, 138, pp.111458. (doi: 10.1016/j.indcrop.2019.06.021)

CommunicationsOral presentations

Hehn A. (2020, April). The study of the secondary metabolism of plants. «Plants and metabolic engineering: an alliance to produce Biomolecules with high added value ». Technical meeting «BioMolecules with high added values». Pôle de compétitivité IAR (Paris).

Hehn A. (2019, November) Biomolecules of plant origin. Agrovalor Meeting. (Nancy)

Larbat R. (2018, May) Therapeutic activities of specialized metabolites involved in the tomato / Tuta absoluta interaction. Future Biomolecules conference (Nancy)

Laurain-Mattar D. (2019, May) Alternative sources of Amaryllidaceae alka-loids. Phytoday –(Bruxelles)

26

Annexe 2List of publications, oral communications, patents etc.

Work-Package 1 : Biotic interactions and biosynthetic pathways

Publications

Munakata R., Olry A., Takemura T., Tatsumi K., Ichino T., Kageyama J., T. Kurata, Sakamoto T., Nakayasu M., Mizutani M., Jacob F., Yamamoto H., Koeduka T., Moriyoshi E., Grosjean J., Krieger C., Sugiyama A., Bourgaud F., Hehn A., and Yazaki K. Parallel evolution of UbiA superfamily proteins into aromatic O-prenyltransferases in plants. (2021) PNAS (Sous presse)

Nicault M., Tidjani A-R., Gauthier A., Dumarcay S., Gelhaye E., Bontemps C., Leblond P. Elicitation of antimicrobially active compounds by Strep-tomyces-Fungus co-cultures. (2021) Microorganisms 9(1), 178 (DOI 10.3390/microorganisms9010178)

Spina R., Saliba S., Dupire F., Ptak A., Hehn A., Piutti S., Poinsignon S. , Leclerc S., Bouguet-Bonnet S. , Laurain-Mattar D. Molecular Identification of Endophytic Bacteria in Leucojum aestivum In Vitro Culture, NMR-Based Metabolomics Study and LC-MS Analysis Leading to Potential Amaryllidaceae Alkaloid Production. (2021) International Journal of Molecular Sciences, 22 (4), pp.1773. (DOI: 10.3390/ijms22041773).

Munakata Y., Gavira C., Genestier J., Bourgaud F., Hehn A., Slezack-Des-chaumes S. Composition and functional comparison of vetiver root endo-phytic microbiota originating from different geographic locations that show antagonistic activity towards Fusarium graminearum (2021) Microbiological Research 243, 126650 (doi.org/10.1016/j.micres.2020.126650)

Miguel S., Legrand G., Duriot L., Delporte M., Menin B., Michel C., Olry A., Chataigné G., Salwinski A., Bygdell J., Vercaigne D., Wingsle G., Hilbert J.L., Bourgaud F., Hehn A., Gagneul D.. Recombinant GDSL lipase-like protein from Ipomoea batatas expressed in Pichia pastoris for an efficient biosynthesis of 3,5-dicaffeoylquinic acid, a promising anti-viral and anti-inflammatory candidate. (2020) Communications biology, 3, 673 (DOI 10.1038/s42003-020-01387-1)

Nicault M., Tidjani A-R., Gauthier A., Dumarcay S., Gelhaye E., Bontemps C., Leblond P. (2020) Mining the biosynthetic potential for specialized meta-bolism of a Streptomyces soil community. MBPI 9(5), 271 (DOI: 10.3390/antibiotics9050271)

Khiralla A., R. Spina, Varbanov V., Philippot S., Lemiere P., Slezack-Des-chaumes S., André P., Mohamed I., Yagi S., Laurain-Mattar D. “Evaluation of Antiviral, Antibacterial and Antiproliferative Activities of the Endophytic Fungus Curvularia papendorfii, and Isolation of a New Polyhydroxyacid (2020), Microorganisms, 8 (9), pp.1353 (doi: 10.3390/microorganisms8091353)

Hemmati N., Azizi S., Spina R., Dupire F., Arouei H. , Saeedi M., Laurain-Mat-tar,D. Accumulation of ajmalicine and vinblastine in cell cultures is enhanced by endophytic fungi of Catharanthus roseus cv. Icy Pink (2020), Ind. Crops Prod., Elsevier, 158, pp.112776 (doi: 10.1016/j.indcrop.2020.112776)

Limones-Mendez M., Dugrand-Judek A., Villard C., Coqueret V., Froelicher Y., Bourgaud F., Olry A., Hehn A. Convergent evolution leading to the appearance of furanocoumarins in citrus plants. (2020) Plant science, 92. (doi. 10.1016/j.plantsci.2019.110392

Munakata R., Kitajima S., Nuttens A., Tatsumi K., Takemura T., Ichino T., Galati G., Vautrin S., Berges H., Grosjean J., Bourgaud F., Sugiyama A., Hehn A., Yazaki K.. Convergent evolution of the UbiA superfamily underlies inde-pendent acquisitions of furanocoumarins in plants. (2019) New Phytologist. 225: pp 2166–2182 (doi: 10.1111/nph.16277)

Munakata R., Takemura T., Tatsumi K., Moriyoshi E., Yanagihara K., Sugiyama A., Suzuki H., Seki H., Muranaka T., Kawano N., Yoshimatsu K., Kawahara N., Yamaura T., Grosjean J., Bourgaud F., Hehn A., Yazaki K. Isolation of Artemi-sia capillaris membrane-bound di-prenyltransferase for phenylpropanoids and redesign of artepillin C in yeast. (2019) Communications Biology. 2, p 384. (doi: 10.1038/s42003-019-0630-0

Galati G., Gandin A., Jolivet Y., Larbat R., Hehn A. Untargeted metabolomics approach reveals diverse responses of Pastinaca sativa to ozone and woun-ding stresses. (2019) Metabolites, 9, p153. (doi: 10.3390/metabo9070153.)

Villard C., Larbat R., Munakata R., Hehn A. Defence mechanisms of Ficus: pyramiding strategies to cope with stressing environments. (2019) Planta. 249(3) pp617-633 (doi: 10.1007/s00425-019-03098-2

Tarakemeh A., Azizi M., Rowshan V., Salehi H., Spina R., Dupire F., Arouie H., Laurain-Mattar D.. Screening of Amaryllidaceae alkaloids in bulbs and tissue cultures of Narcissus papyraceus and four varieties of N. tazetta. (2019) Journal of Pharmaceutical and Biomedical Analysis 172, pp.230-237. (doi: 10.1016/j.jpba.2019.04.043)

Publications in peer-reviewed journals whose topic is related to WP1:Roumani M., Duval R., Ropars A., Risler A., Robin C, Larbat R. Phenolamides : Plant specialized metabolites with a wide range of promising pharmacological and health-promoting interests (2020) Biomedicine and Pharmacotherapy, 131: 110762 (doi.org/10.1016/j.biopha.2020.110762)

Nicault M., Tidjani A-R., Gelhaye E., Bontemps C., Leblond P. Genome se-quence of 5 Streptomyces isolates from a community at microscale. (2020) MRA Volume 9 Issue 23 (DOI : 10.1128/MRA.00428-20)

Ptak A., Simlat M., Morańska E., Skrzypek E., Warchoł M., Tarakemeh A., Laurain-Mattar D. Exogenous melatonin stimulated Amaryllidaceae alkaloid biosynthesis in in vitro cultures of Leucojum aestivum L. (2019) Industrial Crops and Products, 138, pp.111458. (doi: 10.1016/j.indcrop.2019.06.021)


Hehn A. (2020, April). The study of the secondary metabolism of plants. «Plants and metabolic engineering: an alliance to produce Biomolecules with high added value ». Technical meeting «BioMolecules with high added values». Pôle de compétitivité IAR (Paris).

Hehn A. (2019, November) Biomolecules of plant origin. Agrovalor Meeting. (Nancy)

Larbat R. (2018, May) Therapeutic activities of specialized metabolites involved in the tomato / Tuta absoluta interaction. Future Biomolecules conference (Nancy)

Laurain-Mattar D. (2019, May) Alternative sources of Amaryllidaceae alka-loids. Phytoday –(Bruxelles)

27


Roumani M. (2019, May) Impact of modulating the level of phenolamides responsive to Tuta absoluta in tomato and therapeutic interests of these specialized metabolites. 10th MetaSP network meeting (Nancy)

Laurain-Mattar D. (2019, April) The ethnopharmacology associated with phytochemistry, an inexhaustible source of molecules of interest. Young scientist day, 3rd LAE, Plant promotion: between research and innovation (Vandoeuvre lès Nancy)

Spina, R. (2019, Otober) « Application of metabolomics to the study of plant-endophyte interactions in Amaryllidaceae plants. «, Journées scien-tifiques sur les substances naturelles au Centre d’Etudes et de Recherche de Djibouti (CERD), Djibouti.

Hehn A. (2019, April) Endophytes and specialized metabolites as Biocontrole solutions. Biocontrole meeting (Reims).

Villard C. (2019, February). How do plants cope with pests and pathogens ? SIRENA Doctoral School meeting (Nancy).

Nicault M. (2018, May). Bacteria/fungi interactions: a way to induce meta-bolites production, Biomolecule meeting (Nancy).

Posters

Munakata Y., Genestier J., Gavira C., Bourgaud F., Spina R., Slezack-Des-chaumes S., Laurain-Mattar D., Hehn A.. (2020, February 13) Characterization of bacterial endophytes as biocontrol agents and bioactive compound producers with agronomical and pharmaceutical interests. SIRENA Doctoral School meeting (Nancy)

Munakata Y., Gavira C., BourgaudF., Hehn A., Slezack-Deschaumes S. (2019, November 05-08) Characterisation of endophytic bacteria from vetiver roots as biocontrol agents against Fusarium graminearum. IXième AFEM meeting (Bussang)

Munakata Y., Gavira C., Genestier J., Hehn A., Bourgaud F., Slezack-Des-chaumes S. (2019, October 23-25) Characterization of Vetiver endophytic bacteria in taxonomical compositions and antifungal activities against Fusarium graminearum. International conference « Biomolecules: Research & Development, Markets and Acceptability (Palma de Majorque)

Munakata R. (2019, October 23-25) Isolation of a di-prenyltransferase gene from Artemisia capillaris and redesign of bioactive prenylated phenylpro-panes in yeast. International conference « Biomolecules: Research & Development, Markets and Acceptability (Palma de Majorque)

Nicault, M. Bontemps C., Gelhaye E., Leblond P. (2019) Elicitation of cryptic secondary metabolites by microbial co-cultures. FEM (Glasgow)

Nicault M., Bontemps C., Gelhaye E., Leblond P. (2018, May) Bacteria/fungi interactions: a way to induce metabolites production. Future Biomolecules Meeting (Nancy)

Deveau A., Canihac I., Bontemps C. (2018, May 30-31) BioTruf - The truffle microbiota: a new source of biomolecules? Future Biomolecules Meeting, (Nancy)

Munakata R., Takemura T., Sugiyama A., Suzuki H., Seki H., Muranaka T., Yamaura T., Hehn A., Yazaki K.. (2018, March 28-30) A phenylpropane-specific prenyltransferase catalyzes stepwise prenylations in Artemisia capillaris. 59th Annual Meeting of the Japanese Society of Plant Physiologists (Sapporo)

Nicault M., Bontemps C., Gelhaye E., Leblond P. (2018, January) Deciphe-ring and exploiting bacteria-fungi interactions to identify new bioactives metabolites. 12th phytopathology and mycology meetings (Aussois)

Work-Package 2: Bioprocess and chemical engineering for the rational exploitation of natural substances

Publications

Bourkaib, C., Delaunay, S., Framboisier, X., Hôtel, L., Aigle, B., Humeau, C., Guiavarc’h, & Chevalot, I. (2020). N-acylation of L-amino acids in aqueous media: Evaluation of the catalytic performances of Streptomyces ambo-faciens aminoacylases. Enzyme and Microbiology. 137, 109536. https://doi.org/10.1016/j.enzmictec.2020.109536

Bourkaib, C., Guiavarc’h, Y., Chevalot, I., Delaunay, S., Gleize, J., Ghanbaja, J., Valsaque, F., Berrada, N., Desforges, A., & Vigolo, B. (2019). Non-covalent and covalent immobilization of Candida antarctica lipase B on chemically modified multiwalled carbon nanotubes for a green acylation process in supercritical CO2. Catal. Today, 348, 26-36. https://doi.org/10.1016/j.cattod.2019.08.046

Dettori, L., Vibert, F., Guiavarc’h, Y., Delaunay, S., Humeau, C., Blin, J-L., & Chevalot, I. (2018). N-α-acylation of lysine catalyzed by immobilized ami-noacylases from Streptomyces ambofaciens in aqueous medium. Micropor. Mesopor. Mat. 267, 24-34. https://doi.org/10.1016/j.micromeso.2018.03.018

Dettori, L., Ferrari, F., Framboisier, X., Paris, C., Guiavarc’h, Y., Hôtel, L., Aymes, A., Leblond, P., Humeau, C., Kapel, R., Chevalot, I., Aigle, B., & De-launay, S. (2018). An aminoacylase activity from Streptomyces ambofaciens catalyzes the acylation of lysine on α‐position and peptides on N‐terminal position. Eng. Life Sci. 18, 589-599. https://doi.org/10.1002/elsc.201700173

Umerska, A., Gaucher, C., Oyarzun-Ampuero, F., Fries-Raeth, I., Colin, F., Villamizar-Sarmiento, M. G., Maincent, P., & Sapin-Minet, A. (2018). Polymeric nanoparticles for increasing oral bioavailability of curcumin. Antioxidants 7, pii: E46. https://doi.org/10.3390/antiox7040046

Publications in peer-reviewed journals whose topic is related to WP2:Ali, H. H., Michaux, F., Khanji, A. N., Jasniewski, J., & Linder, M. (2018). Chitosan - Shea butter solid nanoparticles assemblies for the preparation of a novel nanoparticles in microparticles system containing curcumin. Colloids Surf. A 553, 359-367. https://doi.org/10.1016/j.colsurfa.2018.05.075

Chebil, A., Léonard, M., Six, J.-L., Nouvel, C., & Durand, A. (2018). Nanopar-ticulate delivery systems for alkyl gallates: influence of the elaboration process on particle characteristics, drug encapsulation and in-vitro release. Colloids Surf. B Biointerfaces 162, 51-361. https://doi.org/ 10.1016/j.colsurfb.2017.11.050

Dettori, L., Jelsch, C., Guiavarc’h, Y., Delaunay, S., Framboisier, X., Chevalot, I., & Humeau, C. (2018). Molecular rules for selectivity in lipase-catalysed acylation of lysine. Process Biochem. 74, 50-60. https://doi.org/ 10.1016/j.procbio.2018.07.021.

El Founi, M., S. Mehawed Abdellatif Soliman, S., Vanderesse, R., Acherar, S., Guedon, E., Chevalot, I., Babin, J., & Six, J.-L. (2018). Light-sensitive dextran-covered PNBA nanoparticles as triggered drug delivery systems: Formulation, characteristics and cytotoxicity. J. Coll. Interface Sci. 514, 289-298. https://doi.org /10.1016/j.jcis.2017.12.036

Khanji, A. N., Michaux, F., Petit, J., Salameh, D., Rizk, T., Jasniewski, J., & Banon, S. (2018). Structure, gelation, and antioxidant properties of curcu-min-doped casein micelle powder produced by spray-drying. Food Funct. 9, 971-981. https://doi.org /10.1039/c7fo01923h

Khanji, A. N., Michaux, F., Salameh, D., Rizk,, T., Banon, S., & Jasniewski, J. (2018). The study of curcumin interaction with micellar casein and lactic acid bacteria cell envelope. LWT 91, 293-302. https://doi.org/10.1016/j.lwt.2018.01.067

27


Roumani M. (2019, May) Impact of modulating the level of phenolamides responsive to Tuta absoluta in tomato and therapeutic interests of these specialized metabolites. 10th MetaSP network meeting (Nancy)

Laurain-Mattar D. (2019, April) The ethnopharmacology associated with phytochemistry, an inexhaustible source of molecules of interest. Young scientist day, 3rd LAE, Plant promotion: between research and innovation (Vandoeuvre lès Nancy)

Spina, R. (2019, Otober) « Application of metabolomics to the study of plant-endophyte interactions in Amaryllidaceae plants. «, Journées scien-tifiques sur les substances naturelles au Centre d’Etudes et de Recherche de Djibouti (CERD), Djibouti.

Hehn A. (2019, April) Endophytes and specialized metabolites as Biocontrole solutions. Biocontrole meeting (Reims).

Villard C. (2019, February). How do plants cope with pests and pathogens ? SIRENA Doctoral School meeting (Nancy).

Nicault M. (2018, May). Bacteria/fungi interactions: a way to induce meta-bolites production, Biomolecule meeting (Nancy).

Posters

Munakata Y., Genestier J., Gavira C., Bourgaud F., Spina R., Slezack-Des-chaumes S., Laurain-Mattar D., Hehn A.. (2020, February 13) Characterization of bacterial endophytes as biocontrol agents and bioactive compound producers with agronomical and pharmaceutical interests. SIRENA Doctoral School meeting (Nancy)

Munakata Y., Gavira C., BourgaudF., Hehn A., Slezack-Deschaumes S. (2019, November 05-08) Characterisation of endophytic bacteria from vetiver roots as biocontrol agents against Fusarium graminearum. IXième AFEM meeting (Bussang)

Munakata Y., Gavira C., Genestier J., Hehn A., Bourgaud F., Slezack-Des-chaumes S. (2019, October 23-25) Characterization of Vetiver endophytic bacteria in taxonomical compositions and antifungal activities against Fusarium graminearum. International conference « Biomolecules: Research & Development, Markets and Acceptability (Palma de Majorque)

Munakata R. (2019, October 23-25) Isolation of a di-prenyltransferase gene from Artemisia capillaris and redesign of bioactive prenylated phenylpro-panes in yeast. International conference « Biomolecules: Research & Development, Markets and Acceptability (Palma de Majorque)

Nicault, M. Bontemps C., Gelhaye E., Leblond P. (2019) Elicitation of cryptic secondary metabolites by microbial co-cultures. FEM (Glasgow)

Nicault M., Bontemps C., Gelhaye E., Leblond P. (2018, May) Bacteria/fungi interactions: a way to induce metabolites production. Future Biomolecules Meeting (Nancy)

Deveau A., Canihac I., Bontemps C. (2018, May 30-31) BioTruf - The truffle microbiota: a new source of biomolecules? Future Biomolecules Meeting, (Nancy)

Munakata R., Takemura T., Sugiyama A., Suzuki H., Seki H., Muranaka T., Yamaura T., Hehn A., Yazaki K.. (2018, March 28-30) A phenylpropane-specific prenyltransferase catalyzes stepwise prenylations in Artemisia capillaris. 59th Annual Meeting of the Japanese Society of Plant Physiologists (Sapporo)

Nicault M., Bontemps C., Gelhaye E., Leblond P. (2018, January) Deciphe-ring and exploiting bacteria-fungi interactions to identify new bioactives metabolites. 12th phytopathology and mycology meetings (Aussois)

Work-Package 2: Bioprocess and chemical engineering for the rational exploitation of natural substances

Publications

Bourkaib, C., Delaunay, S., Framboisier, X., Hôtel, L., Aigle, B., Humeau, C., Guiavarc’h, & Chevalot, I. (2020). N-acylation of L-amino acids in aqueous media: Evaluation of the catalytic performances of Streptomyces ambo-faciens aminoacylases. Enzyme and Microbiology. 137, 109536. https://doi.org/10.1016/j.enzmictec.2020.109536

Bourkaib, C., Guiavarc’h, Y., Chevalot, I., Delaunay, S., Gleize, J., Ghanbaja, J., Valsaque, F., Berrada, N., Desforges, A., & Vigolo, B. (2019). Non-covalent and covalent immobilization of Candida antarctica lipase B on chemically modified multiwalled carbon nanotubes for a green acylation process in supercritical CO2. Catal. Today, 348, 26-36. https://doi.org/10.1016/j.cattod.2019.08.046

Dettori, L., Vibert, F., Guiavarc’h, Y., Delaunay, S., Humeau, C., Blin, J-L., & Chevalot, I. (2018). N-α-acylation of lysine catalyzed by immobilized ami-noacylases from Streptomyces ambofaciens in aqueous medium. Micropor. Mesopor. Mat. 267, 24-34. https://doi.org/10.1016/j.micromeso.2018.03.018

Dettori, L., Ferrari, F., Framboisier, X., Paris, C., Guiavarc’h, Y., Hôtel, L., Aymes, A., Leblond, P., Humeau, C., Kapel, R., Chevalot, I., Aigle, B., & De-launay, S. (2018). An aminoacylase activity from Streptomyces ambofaciens catalyzes the acylation of lysine on α‐position and peptides on N‐terminal position. Eng. Life Sci. 18, 589-599. https://doi.org/10.1002/elsc.201700173

Umerska, A., Gaucher, C., Oyarzun-Ampuero, F., Fries-Raeth, I., Colin, F., Villamizar-Sarmiento, M. G., Maincent, P., & Sapin-Minet, A. (2018). Polymeric nanoparticles for increasing oral bioavailability of curcumin. Antioxidants 7, pii: E46. https://doi.org/10.3390/antiox7040046

Publications in peer-reviewed journals whose topic is related to WP2:Ali, H. H., Michaux, F., Khanji, A. N., Jasniewski, J., & Linder, M. (2018). Chitosan - Shea butter solid nanoparticles assemblies for the preparation of a novel nanoparticles in microparticles system containing curcumin. Colloids Surf. A 553, 359-367. https://doi.org/10.1016/j.colsurfa.2018.05.075

Chebil, A., Léonard, M., Six, J.-L., Nouvel, C., & Durand, A. (2018). Nanopar-ticulate delivery systems for alkyl gallates: influence of the elaboration process on particle characteristics, drug encapsulation and in-vitro release. Colloids Surf. B Biointerfaces 162, 51-361. https://doi.org/ 10.1016/j.colsurfb.2017.11.050

Dettori, L., Jelsch, C., Guiavarc’h, Y., Delaunay, S., Framboisier, X., Chevalot, I., & Humeau, C. (2018). Molecular rules for selectivity in lipase-catalysed acylation of lysine. Process Biochem. 74, 50-60. https://doi.org/ 10.1016/j.procbio.2018.07.021.

El Founi, M., S. Mehawed Abdellatif Soliman, S., Vanderesse, R., Acherar, S., Guedon, E., Chevalot, I., Babin, J., & Six, J.-L. (2018). Light-sensitive dextran-covered PNBA nanoparticles as triggered drug delivery systems: Formulation, characteristics and cytotoxicity. J. Coll. Interface Sci. 514, 289-298. https://doi.org /10.1016/j.jcis.2017.12.036

Khanji, A. N., Michaux, F., Petit, J., Salameh, D., Rizk, T., Jasniewski, J., & Banon, S. (2018). Structure, gelation, and antioxidant properties of curcu-min-doped casein micelle powder produced by spray-drying. Food Funct. 9, 971-981. https://doi.org /10.1039/c7fo01923h

Khanji, A. N., Michaux, F., Salameh, D., Rizk,, T., Banon, S., & Jasniewski, J. (2018). The study of curcumin interaction with micellar casein and lactic acid bacteria cell envelope. LWT 91, 293-302. https://doi.org/10.1016/j.lwt.2018.01.067

28

Moraux, T., Dumarçay, S., Gérardin, P., & Gérardin-Charbonnier, C. (2017). Derivatives of the Lignan 7′-Hydroxymatairesinol with Antioxidant Properties and Enhanced Lipophilicity. J. Nat. Prod. 80, 1783-1790. https://doi.org/10.1021/acs.jnatprod.6b01124

Roy, A., Murcia Valderrama, M. A., Daujat, V., Ferji, K., Léonard, M., Durand, A., Babin, J., Six J.-L. (2018). Stability of biodegradable microcarriers’ surface: physically adsorbed versus chemically linked shells. J. Mater. Chem. B 6, 5130-5143. https://doi.org/10.1039/C8TB01255E


Eid, G., Virot-humeau, C. Chapuis, H., Chebil, L., & Gerardin-Charbonnier, C. (2019, November). Chemical and enzymatic modifications of anti-oxidant wood extractibles. Oral presentation at WOODCHEM 2019, Nancy (France)

Eid, G., Virot-humeau, C. Chapuis, H., Chebil, L., & Gerardin-Charbonnier, C. (2019, October). Functionnalisation of xylo-sourced extractibles by hemisynthesis and enzymatic catalysis. Oral presentation at Conference LUE Biomolecules, Palma de Majorca (Spain).

Bourkaib, C., Vigolo, B., Guiavarc’h, Y., Delaunay, S., Desforges, A., Humeau, C., Blin, J.-L., & Chevalot I. (2019, September). Hydrolytic enzymes immobili-zation on silicate mesoporous materials or carbon nanomaterials: impact on N-acylation performances. Oral presentation at 5th EUROPEAN CONGRESS OF APPLIED BIOTECHNOLOGY, - Florence (Italy)

Rangel Euzcateguy, G., Parajua-Sejil, C., Chapron, D., Marchal, P., Pickaert, G., & Durand, A. (2019, June). Formulation of mixed molecular/macromo-lecular hydrogels based on modified aminoacids and polysaccharide. Oral presentation at Formula X, Manchester (UK).

Posters

Ferji, K., Chapuis H., Six, J.-L., & Gerardin-Charbonnier C. November 20 - 22 (2019, November). High Valorization of Oak Hydrolysable Tannins: Vectorization of Antioxidant Gallic Acid via Bioactive Polymer Materials for Medical Applications. WoodChem 2019, Nancy (France)

Work-Package 3: Functional assessment of new chemical entities

Publications Alhasan, R., Kharma, A., Leroy, P., Jacob, C., & Gaucher C. (2019) Selenium donors at the junction of inflammatory diseases. Current Pharmaceutical Design, 25, 1707-1716. https://doi.org/10.2174/1381612825666190701153903

Bonetti, J., Zhou, Y., Parent, M., Clarot, I., Yu, H., Fries-Raeth, I., Leroy, P., Lartaud, I., & Gaucher, C. (2018). Intestinal absorption of S-nitrosothiols: permeability and transport mechanisms.Biochem. Pharma. 155, 21-31. https://doi.org/10.1016/j.bcp.2018.06.018

Bonetti J., Corti A., Lerouge L., Pompella A., Gaucher C. Phenotypic modu-lation of macrophages and vascular smooth muscle cells in atherosclerosis – Nitro-redox interconnections. Antioxidants-Basel 2021, In press.

Bouressam, M.L., Lecat, S., Raoul, A., Gaucher, C., Perrin-Sarrado, C., Lar-taud, I., & Dupuis, F. (2019) S-Nitrosoglutathione inhibits cerebrovascular angiotensin II dependent and independent AT1 receptor responses: a possible role of S-Nitrosation. British Journal of Pharmacology, 176, 2049-2062. https://doi.org/10.1111/bph.14644

El Hajj A, Yen FT, Oster T, Malaplate C, Pauron L, Corbier C, Lanhers MC, Claudepierre T. (2019) Age-related changes in regiospecific expression of lipolysis stimulated receptor (LSR) in mice brain. PLoS One. 14(6):e0218812. https://doi.org/10.1371/journal.pone.0218812

Fonte, F., Kaminski, S., Vanet, A., Lanfumey, L., Cohen-Salmon, C., Ghislin, S., & Frippiat J.-P. (2019). Socio-environmental stressors encountered during spaceflight partially affect the murine TCRb repertoire and increase its self-reactivity. FASEB Journal, 33, 896-908. https:// doi.org/10.1096/fj.201800969R

Kriznik, A., Libiad, M., Le Cordier, H., Boukhenouna, S., Toledano, M.B., & Rahuel-Clermont, S. (2020) Dynamics of a Key Conformational Transition in the Mechanism of Peroxiredoxin Sulfinylation. ACS Catal., 10, 3326-3339. https://doi.org/10.1021/acscatal.9b04471

Tascher, G., Gerbaix, M., Maes, P., Chazarin, B., Ghislin, S., Antropova, E., Vassilieva, G., Ouzren-Zarhloul, N., Gauquelin-Koch, G., Vico, L., Frippiat J.-P.*, & Bertile F* (2019). Proteomics analysis of femurs from mice embarked on board BION-M1 biosatellite reveals worsened decrease of B lymphopoiesis after one week of recovery on Earth. FASEB Journal, 33, 3772-3783. https://doi.org/10.1096/fj.201801463R *Codirected this study.

Zhou Y., Gaucher C., Fries I., Hobbekaya M., Martin C., Léonard C., Deschamps F., Sapin-Minet A., Parent M. Three different particles to protect and deliver Snitrosoglutathione: nanoparticles, water-in-oil-in-water microparticles and solid-in-oil-in-water microparticles. Nitric Oxide, 2020, 104-105:1-10. (common with WP2)

News and Views in which LUE is acknowledged in HAL

Rahuel-Clermont, S., & Toledano, M. (2018) Parsing protein sulfinic acid switches. Nature Chemical Biology, 14, 991-993. https://doi.org/10.1038/s41589-018-0151-z

Publications whose topic is related to WP3

Bersweiler, A., D’Autréaux, B., Mazon, H., Kriznik, A., Belli, G., Delaunay-Moi-san, A., Toledano, M.B., & Rahuel-Clermont, S. (2017) A scaffold protein that chaperones a cysteine-sulfenic acid in H2O2 signaling. Nature Chemical Biology, 13, 909 – 915. https://doi.org/10.1038/nCHeMBIO.2412

Lec, J.C., Boutserin, S., Mazon, H., Mulliert, G., Boschi-Muller, S. & Talfour-nier, F. (2018) Unraveling the Mechanism of Cysteine Persulfide Formation Catalyzed by 3-Mercaptopyruvate Sulfurtransferases. ACS Catalysis, 8, 2049 – 2059. https://doi.org/10.1021/acscatal.7b02432

Rahuel-Clermont, S., Bchini, R., Barbe, S., Boutserin, S., Andre, I., & Talfour-nier, F. (2019) Enzyme Active Site Loop Revealed as a Gatekeeper for Cofactor Flip by Targeted Molecular Dynamics Simulations and FRET-Based Kinetics. ACS Catalysis, 9, 1337-1346. https://doi.org/10.1021/acscatal.8b03951

Deng, C., Bianchi, A., Presle, N., Moulin, D., Koufany, M., Guillaume, C., Kempf, H., & Pizard, A. (2018) Eplerenone treatment alleviates the development of joint lesions in a new rat model of spontaneous metabolic-associated osteoarthritis. Annals of the Rheumatic Diseases, 77, 315 – 316. https://doi.org/10.1136/annrheumdis-2016-210700

Kokten, T., Gibot, S., Lepage, P., D’Alessio, S., Hablot, J., Ndiaye, N.C., Bus-by-Venner, H., Monot, C., Garnier, B., Moulin, D., Jouzeau, J.Y., Hansmannel, F., Danese, S., Guéant, J.L., Muller, S., & Peyrin-Biroulet, L. (2018) TREM-1 Inhibition Restores Impaired Autophagy Activity and Reduces Colitis in Mice. Journal of Crohn’s and Colitis, 12, 230-244. https://doi.org/10.1093/ecco-jcc/jjx129

Pouillon, L., Bossuyt, P., Vanderstukken, J., Moulin, D., Netter, P., Danese, S., Jouzeau, J.Y., Loeuille, D., & Peyrin-Biroulet, L. (2017) Management of patients with inflammatory bowel disease and spondyloarthritis. Expert Review of Clinical Pharmacology, 10, 1363-1374. https://doi.org/10.1080/17512433.2017.1377609

28

Moraux, T., Dumarçay, S., Gérardin, P., & Gérardin-Charbonnier, C. (2017). Derivatives of the Lignan 7′-Hydroxymatairesinol with Antioxidant Properties and Enhanced Lipophilicity. J. Nat. Prod. 80, 1783-1790. https://doi.org/10.1021/acs.jnatprod.6b01124

Roy, A., Murcia Valderrama, M. A., Daujat, V., Ferji, K., Léonard, M., Durand, A., Babin, J., Six J.-L. (2018). Stability of biodegradable microcarriers’ surface: physically adsorbed versus chemically linked shells. J. Mater. Chem. B 6, 5130-5143. https://doi.org/10.1039/C8TB01255E


Eid, G., Virot-humeau, C. Chapuis, H., Chebil, L., & Gerardin-Charbonnier, C. (2019, November). Chemical and enzymatic modifications of anti-oxidant wood extractibles. Oral presentation at WOODCHEM 2019, Nancy (France)

Eid, G., Virot-humeau, C. Chapuis, H., Chebil, L., & Gerardin-Charbonnier, C. (2019, October). Functionnalisation of xylo-sourced extractibles by hemisynthesis and enzymatic catalysis. Oral presentation at Conference LUE Biomolecules, Palma de Majorca (Spain).

Bourkaib, C., Vigolo, B., Guiavarc’h, Y., Delaunay, S., Desforges, A., Humeau, C., Blin, J.-L., & Chevalot I. (2019, September). Hydrolytic enzymes immobili-zation on silicate mesoporous materials or carbon nanomaterials: impact on N-acylation performances. Oral presentation at 5th EUROPEAN CONGRESS OF APPLIED BIOTECHNOLOGY, - Florence (Italy)

Rangel Euzcateguy, G., Parajua-Sejil, C., Chapron, D., Marchal, P., Pickaert, G., & Durand, A. (2019, June). Formulation of mixed molecular/macromo-lecular hydrogels based on modified aminoacids and polysaccharide. Oral presentation at Formula X, Manchester (UK).

Posters

Ferji, K., Chapuis H., Six, J.-L., & Gerardin-Charbonnier C. November 20 - 22 (2019, November). High Valorization of Oak Hydrolysable Tannins: Vectorization of Antioxidant Gallic Acid via Bioactive Polymer Materials for Medical Applications. WoodChem 2019, Nancy (France)

Work-Package 3: Functional assessment of new chemical entities

Publications Alhasan, R., Kharma, A., Leroy, P., Jacob, C., & Gaucher C. (2019) Selenium donors at the junction of inflammatory diseases. Current Pharmaceutical Design, 25, 1707-1716. https://doi.org/10.2174/1381612825666190701153903

Bonetti, J., Zhou, Y., Parent, M., Clarot, I., Yu, H., Fries-Raeth, I., Leroy, P., Lartaud, I., & Gaucher, C. (2018). Intestinal absorption of S-nitrosothiols: permeability and transport mechanisms.Biochem. Pharma. 155, 21-31. https://doi.org/10.1016/j.bcp.2018.06.018

Bonetti J., Corti A., Lerouge L., Pompella A., Gaucher C. Phenotypic modu-lation of macrophages and vascular smooth muscle cells in atherosclerosis – Nitro-redox interconnections. Antioxidants-Basel 2021, In press.

Bouressam, M.L., Lecat, S., Raoul, A., Gaucher, C., Perrin-Sarrado, C., Lar-taud, I., & Dupuis, F. (2019) S-Nitrosoglutathione inhibits cerebrovascular angiotensin II dependent and independent AT1 receptor responses: a possible role of S-Nitrosation. British Journal of Pharmacology, 176, 2049-2062. https://doi.org/10.1111/bph.14644

El Hajj A, Yen FT, Oster T, Malaplate C, Pauron L, Corbier C, Lanhers MC, Claudepierre T. (2019) Age-related changes in regiospecific expression of lipolysis stimulated receptor (LSR) in mice brain. PLoS One. 14(6):e0218812. https://doi.org/10.1371/journal.pone.0218812

Fonte, F., Kaminski, S., Vanet, A., Lanfumey, L., Cohen-Salmon, C., Ghislin, S., & Frippiat J.-P. (2019). Socio-environmental stressors encountered during spaceflight partially affect the murine TCRb repertoire and increase its self-reactivity. FASEB Journal, 33, 896-908. https:// doi.org/10.1096/fj.201800969R

Kriznik, A., Libiad, M., Le Cordier, H., Boukhenouna, S., Toledano, M.B., & Rahuel-Clermont, S. (2020) Dynamics of a Key Conformational Transition in the Mechanism of Peroxiredoxin Sulfinylation. ACS Catal., 10, 3326-3339. https://doi.org/10.1021/acscatal.9b04471

Tascher, G., Gerbaix, M., Maes, P., Chazarin, B., Ghislin, S., Antropova, E., Vassilieva, G., Ouzren-Zarhloul, N., Gauquelin-Koch, G., Vico, L., Frippiat J.-P.*, & Bertile F* (2019). Proteomics analysis of femurs from mice embarked on board BION-M1 biosatellite reveals worsened decrease of B lymphopoiesis after one week of recovery on Earth. FASEB Journal, 33, 3772-3783. https://doi.org/10.1096/fj.201801463R *Codirected this study.

Zhou Y., Gaucher C., Fries I., Hobbekaya M., Martin C., Léonard C., Deschamps F., Sapin-Minet A., Parent M. Three different particles to protect and deliver Snitrosoglutathione: nanoparticles, water-in-oil-in-water microparticles and solid-in-oil-in-water microparticles. Nitric Oxide, 2020, 104-105:1-10. (common with WP2)

News and Views in which LUE is acknowledged in HAL

Rahuel-Clermont, S., & Toledano, M. (2018) Parsing protein sulfinic acid switches. Nature Chemical Biology, 14, 991-993. https://doi.org/10.1038/s41589-018-0151-z

Publications whose topic is related to WP3

Bersweiler, A., D’Autréaux, B., Mazon, H., Kriznik, A., Belli, G., Delaunay-Moi-san, A., Toledano, M.B., & Rahuel-Clermont, S. (2017) A scaffold protein that chaperones a cysteine-sulfenic acid in H2O2 signaling. Nature Chemical Biology, 13, 909 – 915. https://doi.org/10.1038/nCHeMBIO.2412

Lec, J.C., Boutserin, S., Mazon, H., Mulliert, G., Boschi-Muller, S. & Talfour-nier, F. (2018) Unraveling the Mechanism of Cysteine Persulfide Formation Catalyzed by 3-Mercaptopyruvate Sulfurtransferases. ACS Catalysis, 8, 2049 – 2059. https://doi.org/10.1021/acscatal.7b02432

Rahuel-Clermont, S., Bchini, R., Barbe, S., Boutserin, S., Andre, I., & Talfour-nier, F. (2019) Enzyme Active Site Loop Revealed as a Gatekeeper for Cofactor Flip by Targeted Molecular Dynamics Simulations and FRET-Based Kinetics. ACS Catalysis, 9, 1337-1346. https://doi.org/10.1021/acscatal.8b03951

Deng, C., Bianchi, A., Presle, N., Moulin, D., Koufany, M., Guillaume, C., Kempf, H., & Pizard, A. (2018) Eplerenone treatment alleviates the development of joint lesions in a new rat model of spontaneous metabolic-associated osteoarthritis. Annals of the Rheumatic Diseases, 77, 315 – 316. https://doi.org/10.1136/annrheumdis-2016-210700

Kokten, T., Gibot, S., Lepage, P., D’Alessio, S., Hablot, J., Ndiaye, N.C., Bus-by-Venner, H., Monot, C., Garnier, B., Moulin, D., Jouzeau, J.Y., Hansmannel, F., Danese, S., Guéant, J.L., Muller, S., & Peyrin-Biroulet, L. (2018) TREM-1 Inhibition Restores Impaired Autophagy Activity and Reduces Colitis in Mice. Journal of Crohn’s and Colitis, 12, 230-244. https://doi.org/10.1093/ecco-jcc/jjx129

Pouillon, L., Bossuyt, P., Vanderstukken, J., Moulin, D., Netter, P., Danese, S., Jouzeau, J.Y., Loeuille, D., & Peyrin-Biroulet, L. (2017) Management of patients with inflammatory bowel disease and spondyloarthritis. Expert Review of Clinical Pharmacology, 10, 1363-1374. https://doi.org/10.1080/17512433.2017.1377609

29


Hablot, J., Peyrin-Biroulet, L., Kokten, T., Omar, R., Netter, P., Bastien, C., Jouzeau, J.Y., Sokol, H., & Moulin, D. (2017) Experimental colitis delays and reduces the severity of collagen-induced arthritis in mice. PLoS ONE, 12, e0184624. https://doi.org/10.1371/journal.pone.0184624

Moulin, D., Salone, V., Koufany, M., Clément, T., Behm-Ansmant, I., Branlant, C., Charpentier, B., & Jouzeau, J.Y. (2017) MicroRNA-29b Contributes to Collagens Imbalance in Human Osteoarthritic and Dedifferentiated Articular Chondrocytes. BioMed Research International, 2017, 1 – 12. https://doi.org/10.1155/2017/9792512

Gross, J.B., Guillaume, C., Gegout-Pottie, P., Reboul, P., Jouzeau, J.Y., Mainard, D., & Presle, N. (2017) The infrapatellar fat pad induces inflammatory and degradative effects in articular cells but not through leptin or adiponectin. Clinical and experimental rheumatology, 35, 53-60.

Hu, Y., Yéléhé-Okouma, M., Ea, H.K., Jouzeau, J.Y., & Reboul, P. (2017) Ga-lectin-3: A key player in arthritis. Joint Bone Spine, 84, 15 – 20. https://doi.org/10.1016/j.jbspin.2016.02.029

Allen, P., Olivera, P., Emery, P., Moulin, D., Jouzeau, J.Y., Netter, P., Danese, S., Feagan, B., Sandborn, W., Peyrin-Biroulet, L., & Allen, B. (2017) Review article: moving towards common therapeutic goals in Crohn’s disease and rheumatoid arthritis. Alimentary Pharmacology & Therapeutics, 45, 1058 – 1072. https://doi.org/10.1111/apt.13995

Kriznik, A., Yelehe-Okouma, M., Lec, J.C., Groshenry, G., Le Cordier, H., Charron, C., Quinternet, M., Mazon, H., Talfournier, F., Boschi-Muller, S., Jouzeau, J.Y., & Reboul, P. (2018) CRD SAT generated by pCARGHO: A new efficient lectin-based affinity tag method for safe, simple and low-cost protein purification. Biotechnology Journal, e1800214. https://doi.org/10.1002/biot.201800214

Harlé, G., Kaminski, S., Dubayle, D., Frippiat, J.-P.*, & Ropars, A* (2018) Murine splenic B cells express corticotropin-releasing hormone receptor 2 that affect their viability during a stress response. Scientific Reports, 8, 143. https://doi.org/10.1038/s41598-017-18401-y *Codirected this study.

D’Agostino, M., Lemmet, T., Dufay, C., Luc, A., Frippiat, J.-P., Machouart, M., & Debourgogne, A. (2018) Overinduction of CYP51A gene after exposure to azole antifungals provides a first clue to resistance mechanism in Fusarium solani species complex. Microbial Drug Resistance, 24, 768-773. https://doi.org/10.1089/mdr.2017.0311

Xie, T., Stahopoulou, M., Akbar, S., Oster, T., Masson, T., Yen, F.T., & Visvikis-Siest, S. (2018) The association of the epistatic interaction of APOE and LSR genetic variants in Alzheimer’s disease. Neurobiology of Aging, 69, 292e1-292e5. https://doi.org/10.1016/j.neurobiolaging.2018.04.01

Xie, T., Stathopoulou, M., Akbar, S., Oster, T., Siest, G., Yen, F.T., & Visvikis-Siest, S. (2018) Effect of LSR polymorphism on blood lipid levels and age-specific epistatic interaction with the APOE common polymorphism. Clinical Genetics, 93, 846-852. https://doi.org/10.1111/cge.13181

Colin, J., Thomas, M., Gregory-Pauron, L., Pincon, A., Lanhers, M.C., Corbier, C., Claudepierre, T., Yen, F.T., Oster, T., & Malaplate-Armand, C. (2017) Main-tenance of membrane organization in the aging mouse brain as determining factor for preventing receptor dysfunction and for improving response to anti-Alzheimer treatments. Neurobiology of Aging, 54, 84-93. https://doi.org/10.1016/j.neurobiolaging.2017.02.015


Rahuel-Clermont, S. (2018, May). Potentialités thérapeutiques de nouvelles biomolécules ; Outils, compétences et domaines d’application. Oral session presentation at Workshop Biomolecules du futur: de la découverte aux applications, Nancy (France).

Rahuel−Clermont, S. (2018, August) Functional evaluation of new biomo-lecules: lab consortium presentation, toolbox, skills and knowhow. Oral session presentation at BASF seminar « IMPACT Biomolecules Project “Nancy (France).

Gaucher, C., Rahuel-Clermont, S. (2020, October) Characterization of active biomolecules. Biomolecules, research and development, market and ac-ceptability. Oral session presentation at the Conference LUE Biomolecules, Palma de Majorca (Spain).

Posters

Sestito, S., Gaucher, C., Ginglinger, J.F., Jouzeau, J.-Y., Boschi-Muller, S., Rahuel-Clermont, S., Moulin, D. (2020, October) Simple targeted cell-based assay for the screening of natural extracts libraries with anti-inflammatory properties. Poster session presentation at the Conference LUE Biomole-cules, Palma de Majorca (Spain).

Safi, H., Mazon, H., Kriznik, A., Le Cordier, H., Touly, N., Yen, F.T., Boschi-Muller, S., Corbier, C. (2020, October) LSR as a new potential target: Purification and characterization of its recombinant and circulating forms. Poster session presentation at the Conference LUE Biomolecules, Palma de Majorca (Spain).

Transversal Action 1: PolyketidesPublications

Su L., Hôtel L., Paris C., Brachmann O., Piel J., Jacob C., Aigle B. & Weissman K. J. (2021) Successes, suprises and pitfalls in modular polyketide synthase engineering: generation of ring-contracted stambomycins. Nature Com-munications. In revision (doi: 10.21203/rs.3.rs-222743/v1).

Massicard J.-M., Soligot C., Weissman K. J. & Jacob C. (2020) Manipulating polyketide stereochemistry by exchange of polyketide synthase modules. Chemical Communications 56(84), 12749. https://doi.org/10.1039/d0cc05068g

Risser F., Collin S., Dos Santos-Morais R., Gruez A., Chagot B. & Weissman K. J. (2020) Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. Journal of Structural Biology, 212(1), 107581. https://doi.org/10.3390/antibiotics7030062

Dorival, J., Risser, F., Jacob, C., Collin, S., Dräeger, G., Paris, C., Chagot, B., Kirschning, A., Gruez, A. & Weissman K. J. (2018). Insights into a dual function amide oxidase/macrocyclase from lankacidin biosynthesis. Nature Communications, 9(1), 3988. https://doi.org/10.1038/s41467-018-06323-w

Vicente, C., Thibessard, A., Lorenzi, J. N., Benhadj, M., Hotel, L., Gacemi-Ki-rane, D., Lespinet, O., Leblond, P. & Aigle B. (2018). Comparative genomics among closely related Streptomyces strains revealed specialized metabolite biosynthetic gene cluster diversity. Antibiotics 7(4), 86. https://doi.org/10.3390/antibiotics7040086


Weissman, K.J. (2019, October). Genome mining: accessing Nature’s natural product diversity. Biomolecules: Research & Development, Markets and Acceptability, Palma de Majorca (Spain).

Weissman, K.J. (2018, May). Polykétides : applications dans le domaine médical et en agrosciences et développement de nouveaux analogues par biologie de synthèse. Séminaire IMPACT Biomolécules Biomolécules du futur : de la découverte aux applications, Nancy (France).

29


Hablot, J., Peyrin-Biroulet, L., Kokten, T., Omar, R., Netter, P., Bastien, C., Jouzeau, J.Y., Sokol, H., & Moulin, D. (2017) Experimental colitis delays and reduces the severity of collagen-induced arthritis in mice. PLoS ONE, 12, e0184624. https://doi.org/10.1371/journal.pone.0184624

Moulin, D., Salone, V., Koufany, M., Clément, T., Behm-Ansmant, I., Branlant, C., Charpentier, B., & Jouzeau, J.Y. (2017) MicroRNA-29b Contributes to Collagens Imbalance in Human Osteoarthritic and Dedifferentiated Articular Chondrocytes. BioMed Research International, 2017, 1 – 12. https://doi.org/10.1155/2017/9792512

Gross, J.B., Guillaume, C., Gegout-Pottie, P., Reboul, P., Jouzeau, J.Y., Mainard, D., & Presle, N. (2017) The infrapatellar fat pad induces inflammatory and degradative effects in articular cells but not through leptin or adiponectin. Clinical and experimental rheumatology, 35, 53-60.

Hu, Y., Yéléhé-Okouma, M., Ea, H.K., Jouzeau, J.Y., & Reboul, P. (2017) Ga-lectin-3: A key player in arthritis. Joint Bone Spine, 84, 15 – 20. https://doi.org/10.1016/j.jbspin.2016.02.029

Allen, P., Olivera, P., Emery, P., Moulin, D., Jouzeau, J.Y., Netter, P., Danese, S., Feagan, B., Sandborn, W., Peyrin-Biroulet, L., & Allen, B. (2017) Review article: moving towards common therapeutic goals in Crohn’s disease and rheumatoid arthritis. Alimentary Pharmacology & Therapeutics, 45, 1058 – 1072. https://doi.org/10.1111/apt.13995

Kriznik, A., Yelehe-Okouma, M., Lec, J.C., Groshenry, G., Le Cordier, H., Charron, C., Quinternet, M., Mazon, H., Talfournier, F., Boschi-Muller, S., Jouzeau, J.Y., & Reboul, P. (2018) CRD SAT generated by pCARGHO: A new efficient lectin-based affinity tag method for safe, simple and low-cost protein purification. Biotechnology Journal, e1800214. https://doi.org/10.1002/biot.201800214

Harlé, G., Kaminski, S., Dubayle, D., Frippiat, J.-P.*, & Ropars, A* (2018) Murine splenic B cells express corticotropin-releasing hormone receptor 2 that affect their viability during a stress response. Scientific Reports, 8, 143. https://doi.org/10.1038/s41598-017-18401-y *Codirected this study.

D’Agostino, M., Lemmet, T., Dufay, C., Luc, A., Frippiat, J.-P., Machouart, M., & Debourgogne, A. (2018) Overinduction of CYP51A gene after exposure to azole antifungals provides a first clue to resistance mechanism in Fusarium solani species complex. Microbial Drug Resistance, 24, 768-773. https://doi.org/10.1089/mdr.2017.0311

Xie, T., Stahopoulou, M., Akbar, S., Oster, T., Masson, T., Yen, F.T., & Visvikis-Siest, S. (2018) The association of the epistatic interaction of APOE and LSR genetic variants in Alzheimer’s disease. Neurobiology of Aging, 69, 292e1-292e5. https://doi.org/10.1016/j.neurobiolaging.2018.04.01

Xie, T., Stathopoulou, M., Akbar, S., Oster, T., Siest, G., Yen, F.T., & Visvikis-Siest, S. (2018) Effect of LSR polymorphism on blood lipid levels and age-specific epistatic interaction with the APOE common polymorphism. Clinical Genetics, 93, 846-852. https://doi.org/10.1111/cge.13181

Colin, J., Thomas, M., Gregory-Pauron, L., Pincon, A., Lanhers, M.C., Corbier, C., Claudepierre, T., Yen, F.T., Oster, T., & Malaplate-Armand, C. (2017) Main-tenance of membrane organization in the aging mouse brain as determining factor for preventing receptor dysfunction and for improving response to anti-Alzheimer treatments. Neurobiology of Aging, 54, 84-93. https://doi.org/10.1016/j.neurobiolaging.2017.02.015


Rahuel-Clermont, S. (2018, May). Potentialités thérapeutiques de nouvelles biomolécules ; Outils, compétences et domaines d’application. Oral session presentation at Workshop Biomolecules du futur: de la découverte aux applications, Nancy (France).

Rahuel−Clermont, S. (2018, August) Functional evaluation of new biomo-lecules: lab consortium presentation, toolbox, skills and knowhow. Oral session presentation at BASF seminar « IMPACT Biomolecules Project “Nancy (France).

Gaucher, C., Rahuel-Clermont, S. (2020, October) Characterization of active biomolecules. Biomolecules, research and development, market and ac-ceptability. Oral session presentation at the Conference LUE Biomolecules, Palma de Majorca (Spain).

Posters

Sestito, S., Gaucher, C., Ginglinger, J.F., Jouzeau, J.-Y., Boschi-Muller, S., Rahuel-Clermont, S., Moulin, D. (2020, October) Simple targeted cell-based assay for the screening of natural extracts libraries with anti-inflammatory properties. Poster session presentation at the Conference LUE Biomole-cules, Palma de Majorca (Spain).

Safi, H., Mazon, H., Kriznik, A., Le Cordier, H., Touly, N., Yen, F.T., Boschi-Muller, S., Corbier, C. (2020, October) LSR as a new potential target: Purification and characterization of its recombinant and circulating forms. Poster session presentation at the Conference LUE Biomolecules, Palma de Majorca (Spain).

Transversal Action 1: PolyketidesPublications

Su L., Hôtel L., Paris C., Brachmann O., Piel J., Jacob C., Aigle B. & Weissman K. J. (2021) Successes, suprises and pitfalls in modular polyketide synthase engineering: generation of ring-contracted stambomycins. Nature Com-munications. In revision (doi: 10.21203/rs.3.rs-222743/v1).

Massicard J.-M., Soligot C., Weissman K. J. & Jacob C. (2020) Manipulating polyketide stereochemistry by exchange of polyketide synthase modules. Chemical Communications 56(84), 12749. https://doi.org/10.1039/d0cc05068g

Risser F., Collin S., Dos Santos-Morais R., Gruez A., Chagot B. & Weissman K. J. (2020) Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. Journal of Structural Biology, 212(1), 107581. https://doi.org/10.3390/antibiotics7030062

Dorival, J., Risser, F., Jacob, C., Collin, S., Dräeger, G., Paris, C., Chagot, B., Kirschning, A., Gruez, A. & Weissman K. J. (2018). Insights into a dual function amide oxidase/macrocyclase from lankacidin biosynthesis. Nature Communications, 9(1), 3988. https://doi.org/10.1038/s41467-018-06323-w

Vicente, C., Thibessard, A., Lorenzi, J. N., Benhadj, M., Hotel, L., Gacemi-Ki-rane, D., Lespinet, O., Leblond, P. & Aigle B. (2018). Comparative genomics among closely related Streptomyces strains revealed specialized metabolite biosynthetic gene cluster diversity. Antibiotics 7(4), 86. https://doi.org/10.3390/antibiotics7040086


Weissman, K.J. (2019, October). Genome mining: accessing Nature’s natural product diversity. Biomolecules: Research & Development, Markets and Acceptability, Palma de Majorca (Spain).

Weissman, K.J. (2018, May). Polykétides : applications dans le domaine médical et en agrosciences et développement de nouveaux analogues par biologie de synthèse. Séminaire IMPACT Biomolécules Biomolécules du futur : de la découverte aux applications, Nancy (France).

30

Aigle, B. (2018, May). L’exemple de la stambomycine, un macrolide à activité antiproliférative, et applications à d’autres voies de biosynthèse. Séminaire IMPACT Biomolécules Biomolécules du futur : de la découverte aux appli-cations, Nancy (France).Su, L. (2018, June). Creating analogs of the anti-tumor polyketide stam-bomycin by genetic engineering and allied approaches. IMoPA departmental seminar, Nancy (France).

Su, L. (2018, January). Creating analogs of the anti-tumor polyketide stambomycin by genetic engineering and allied approaches. Biomolecules project debriefing day, Nancy (France).

Su, L. (2019, February). Creating analogs of the anti-tumor polyketide stambomycin by genetic engineering and allied approaches. Ecole Docto-rale SIReNa, séminaire annuel sur le thème Science et Territoire : vers une nouvelle économie, Nancy (France).

Su, L. (2020, December). Engineering of a polyketide synthase leads to ‘low-fat’ stambomycins. Actino 2020. Journées des actinobactéries. Conférence en ligne.

Posters

Su, L., Hôtel, L., Jacob, C., Weissman, K.J. & Aigle, B. (2018, May). Creating analogues of the anti-tumor polyketide stambomycins by genetic engineering and allied approaches. Séminaire IMPACT Biomolécules Biomolécules du futur : de la découverte aux applications, Nancy (France).

Transversal Action 2: PolyphenolsPublications

Cesari, L., Mutelet, F. & Canabady-Rochelle, L. (2019). Antioxidant proper-ties of phenolic surrogates of lignin depolymerisation. Industrial Crops and Products, 129, 480-487. https://doi.org/10.1016/j.indcrop.2018.12.010

Elmi, A., Spina, R., Risler, A., Philippot, S., Mohamed Abdoul-latif, F., Mérito, A., Duval, R.E., & Laurain-Mattar, D. (2020). Evaluation of Antioxidant and Antibacterial Activities, Cytotoxicity of Acacia seyal Del Bark Extracts and Isolated Compounds. Molecules, 25, 2392. doi:10.3390/molecules25102392

Le, T.T., Aymes, A., Framboisier, X., Ioannou, I., & Kapel, R. (2020). Adsorption of Phenolic Compounds from an Aqueous By-product of Sunflower Protein Extraction/Purification by Macroporous Resins. Journal of Chromatography & Separation Techniques, 11, 435. doi: 10.35248/2157-7064.20.11.435

Nekkaa, A., Benaissa, A., Mutelet, F., & Canabady-Rochelle, L. (2021). Rhamnus alaternus plant: extraction of bioactive fractions and evaluation of their pharmacological and phytochemical properties. Antioxidants, 10, 300. https://doi.org/10.3390/antiox10020300

Resa, M., Mubarok, M., Darmawan, W., Syafii, W. Dumarcay, S., Gérar-din-Charbonnier, C., & Gérardin, P. (2021). Biomolecules of interest present in the main industrial wood species used in Indonesia - A review. Journal of Renewable Materials, 9, 399-449. doi:10.32604/jrm.2020.014286.

D’agostino, M., Tesse, N., Lavergne, R.A., Le Pape, R., Bouchara, J.-L., Frippiat, J.-P., Machouart M., & Debourgogne, A. (2021). Characterization of the antifungal effect of a plant-based compound CIN-102 on the main septal filamentous fungi involved in human pathology. Journal of Global Antimicrobial Resistance, Under revision.

Nekkaa, A., Benaissa, A., Mutelet, F., & Canabady-Rochelle, L. (2021). Opti-mization of the extraction process of bioactive compounds from Rhamnus alaternus leaves using Box-Behnken experimental design. Submitted to Journal of Applied Research in Medicinal and Aromatic Plants.

Elmi, A., Spina, R., Philippot, S., Mohamed Abdoul-latif, F., Mérito, A., & Lau-rain-Mattar, D. (2021). Antioxidant activity, cytotoxicity, toxicity in mices, and chemical constituents of Aloe djiboutiensis leaves. Submitted to Molecules.

Le T.T., Ropars, A., Aymes, A., Frippiat J.-P., & Kapel R. (2021). Multicriteria optimization of phenolic compounds capture from a sunflower protein iso-late production process by-product by adsorption column and assessment of their antioxidant and anti-inflammatory effects. Foods, sous presse.D’agostino, M., Tesse, N., Lavergne, R.A., Le Pape, R., Bouchara JP, Frippiat, J.-P., Machouart, M., & Debourgogne, A. (2021). Characteri-sation of the antifungal effects of a plant-based compound, CIN-102, on the main septal filamentous fungi involved in human pathology. Journal of Global Antimicrobial Resistance, 25, 171-180. https://doi.org/10.1016/j.jgar.2021.03.017

D’agostino, M., Tesse, N., Lavergne, R.A., Le Pape, R., Frippiat, J.-P., Machouart, M., & Debourgogne, A. (2021). Antifungal effect of a new plant-based compound, CIN-102, on filamentous fungi in situations of resistance. Submitted to Journal of Medical Microbiology. In revision

Book chapters in which LUE is acknowledgedFrippiat, J.-P. (2019). Space exploration and travel, future technologies for inflight monitoring and diagnostic. Global Virology volume III (GVIII): Virology in the 21st Century, Springer, pages 471-484.

Le, T.T., Ropars, A., Sundaresan, A., Crucian, B., Choukér, A., & Frippiat J.-P. (2020). Pharmacological countermeasures to spaceflight-induced alterations of the immune system. In: Stress challenges and immunity in space. Second edition, Springer, pages 637-657.

Publications whose topic is related to TA2

Kebbi-Benkeder, Z., Manso, R., Fortin, M., Dumarçay, S., Gérardin, P., & Colin, F. (2017). Knot extractives: a model for analysing the eco-physiological factors that control the within and between-tree variability. Trees, 31, 1619-1633. https://doi.org/10.1007/s00468-017-1573-z

Moraux, T., Dumarcay, S., Gérardin, P., & Gérardin-Charbonnier, C. (2017). Derivatives of the lignan 7’-hydroxymatairesinol with antioxidant properties and enhanced lipophilicity. Journal of Natural Products, 80, 1783-1790. https://doi.org/10.1021/acs.jnatprod.6b01124

Arifiani Rosdiana, N., Dumarçay, S., Gérardin, C., Chapuis, H., Santiago-Me-dina, FJ., Kartika Sari, R., Syafii, W., Gelhaye, E., Raharivelomanana, P., Mohammed, R., & Gérardin, P. (2017). Characterization of bark extractives of different industrial Indonesian wood species for potential valorization. Industrial Crops & Products, 108(1), 121–127. https://doi.org/10.1016/j.indcrop.2017.06.034

Harlé, G., Lalonde, R., Fonte, C., Ropars, A., Frippiat, J.-P., & Strazielle, C. (2017). Repeated corticosterone injections in adult mice alter stress hormonal receptor expression in the cerebellum and motor coordination without affecting spatial learning. Behavioural Brain Research, 326, 121-131. https://doi.org/10.1016/j.bbr.2017.02.035

Arifiani Rosdiana, N., Dumarçay, S., Gérardin, C., Chapuis, H., Santiago-Me-dina, F.J., Kartika Sari, R., Syafii, W., Gelhaye, E., Raharivelomanana, P., Mohammed, R., & Gérardin, P. (2017). Characterization of bark extractives of different industrial Indonesian wood species for potential valorization. Industrial Crops & Products, 108, 121–127. https://doi.org/10.1016/j.indcrop.2017.06.034

Kebbi-Benkeder, Z., Manso, R., Gérardin, P., Dumarcay, S., Chopard, B. & Colin, F. (2017). Knot extractives: a model for analysing the eco-physiolo-gical factors that control the within and between-tree variability. Trees 31, 1619–1633. https://doi.org/10.1007/s00468-017-1573-z

30

Aigle, B. (2018, May). L’exemple de la stambomycine, un macrolide à activité antiproliférative, et applications à d’autres voies de biosynthèse. Séminaire IMPACT Biomolécules Biomolécules du futur : de la découverte aux appli-cations, Nancy (France).Su, L. (2018, June). Creating analogs of the anti-tumor polyketide stam-bomycin by genetic engineering and allied approaches. IMoPA departmental seminar, Nancy (France).

Su, L. (2018, January). Creating analogs of the anti-tumor polyketide stambomycin by genetic engineering and allied approaches. Biomolecules project debriefing day, Nancy (France).

Su, L. (2019, February). Creating analogs of the anti-tumor polyketide stambomycin by genetic engineering and allied approaches. Ecole Docto-rale SIReNa, séminaire annuel sur le thème Science et Territoire : vers une nouvelle économie, Nancy (France).

Su, L. (2020, December). Engineering of a polyketide synthase leads to ‘low-fat’ stambomycins. Actino 2020. Journées des actinobactéries. Conférence en ligne.

Posters

Su, L., Hôtel, L., Jacob, C., Weissman, K.J. & Aigle, B. (2018, May). Creating analogues of the anti-tumor polyketide stambomycins by genetic engineering and allied approaches. Séminaire IMPACT Biomolécules Biomolécules du futur : de la découverte aux applications, Nancy (France).

Transversal Action 2: PolyphenolsPublications

Cesari, L., Mutelet, F. & Canabady-Rochelle, L. (2019). Antioxidant proper-ties of phenolic surrogates of lignin depolymerisation. Industrial Crops and Products, 129, 480-487. https://doi.org/10.1016/j.indcrop.2018.12.010

Elmi, A., Spina, R., Risler, A., Philippot, S., Mohamed Abdoul-latif, F., Mérito, A., Duval, R.E., & Laurain-Mattar, D. (2020). Evaluation of Antioxidant and Antibacterial Activities, Cytotoxicity of Acacia seyal Del Bark Extracts and Isolated Compounds. Molecules, 25, 2392. doi:10.3390/molecules25102392

Le, T.T., Aymes, A., Framboisier, X., Ioannou, I., & Kapel, R. (2020). Adsorption of Phenolic Compounds from an Aqueous By-product of Sunflower Protein Extraction/Purification by Macroporous Resins. Journal of Chromatography & Separation Techniques, 11, 435. doi: 10.35248/2157-7064.20.11.435

Nekkaa, A., Benaissa, A., Mutelet, F., & Canabady-Rochelle, L. (2021). Rhamnus alaternus plant: extraction of bioactive fractions and evaluation of their pharmacological and phytochemical properties. Antioxidants, 10, 300. https://doi.org/10.3390/antiox10020300

Resa, M., Mubarok, M., Darmawan, W., Syafii, W. Dumarcay, S., Gérar-din-Charbonnier, C., & Gérardin, P. (2021). Biomolecules of interest present in the main industrial wood species used in Indonesia - A review. Journal of Renewable Materials, 9, 399-449. doi:10.32604/jrm.2020.014286.

D’agostino, M., Tesse, N., Lavergne, R.A., Le Pape, R., Bouchara, J.-L., Frippiat, J.-P., Machouart M., & Debourgogne, A. (2021). Characterization of the antifungal effect of a plant-based compound CIN-102 on the main septal filamentous fungi involved in human pathology. Journal of Global Antimicrobial Resistance, Under revision.

Nekkaa, A., Benaissa, A., Mutelet, F., & Canabady-Rochelle, L. (2021). Opti-mization of the extraction process of bioactive compounds from Rhamnus alaternus leaves using Box-Behnken experimental design. Submitted to Journal of Applied Research in Medicinal and Aromatic Plants.

Elmi, A., Spina, R., Philippot, S., Mohamed Abdoul-latif, F., Mérito, A., & Lau-rain-Mattar, D. (2021). Antioxidant activity, cytotoxicity, toxicity in mices, and chemical constituents of Aloe djiboutiensis leaves. Submitted to Molecules.

Le T.T., Ropars, A., Aymes, A., Frippiat J.-P., & Kapel R. (2021). Multicriteria optimization of phenolic compounds capture from a sunflower protein iso-late production process by-product by adsorption column and assessment of their antioxidant and anti-inflammatory effects. Foods, sous presse.D’agostino, M., Tesse, N., Lavergne, R.A., Le Pape, R., Bouchara JP, Frippiat, J.-P., Machouart, M., & Debourgogne, A. (2021). Characteri-sation of the antifungal effects of a plant-based compound, CIN-102, on the main septal filamentous fungi involved in human pathology. Journal of Global Antimicrobial Resistance, 25, 171-180. https://doi.org/10.1016/j.jgar.2021.03.017

D’agostino, M., Tesse, N., Lavergne, R.A., Le Pape, R., Frippiat, J.-P., Machouart, M., & Debourgogne, A. (2021). Antifungal effect of a new plant-based compound, CIN-102, on filamentous fungi in situations of resistance. Submitted to Journal of Medical Microbiology. In revision

Book chapters in which LUE is acknowledgedFrippiat, J.-P. (2019). Space exploration and travel, future technologies for inflight monitoring and diagnostic. Global Virology volume III (GVIII): Virology in the 21st Century, Springer, pages 471-484.

Le, T.T., Ropars, A., Sundaresan, A., Crucian, B., Choukér, A., & Frippiat J.-P. (2020). Pharmacological countermeasures to spaceflight-induced alterations of the immune system. In: Stress challenges and immunity in space. Second edition, Springer, pages 637-657.

Publications whose topic is related to TA2

Kebbi-Benkeder, Z., Manso, R., Fortin, M., Dumarçay, S., Gérardin, P., & Colin, F. (2017). Knot extractives: a model for analysing the eco-physiological factors that control the within and between-tree variability. Trees, 31, 1619-1633. https://doi.org/10.1007/s00468-017-1573-z

Moraux, T., Dumarcay, S., Gérardin, P., & Gérardin-Charbonnier, C. (2017). Derivatives of the lignan 7’-hydroxymatairesinol with antioxidant properties and enhanced lipophilicity. Journal of Natural Products, 80, 1783-1790. https://doi.org/10.1021/acs.jnatprod.6b01124

Arifiani Rosdiana, N., Dumarçay, S., Gérardin, C., Chapuis, H., Santiago-Me-dina, FJ., Kartika Sari, R., Syafii, W., Gelhaye, E., Raharivelomanana, P., Mohammed, R., & Gérardin, P. (2017). Characterization of bark extractives of different industrial Indonesian wood species for potential valorization. Industrial Crops & Products, 108(1), 121–127. https://doi.org/10.1016/j.indcrop.2017.06.034

Harlé, G., Lalonde, R., Fonte, C., Ropars, A., Frippiat, J.-P., & Strazielle, C. (2017). Repeated corticosterone injections in adult mice alter stress hormonal receptor expression in the cerebellum and motor coordination without affecting spatial learning. Behavioural Brain Research, 326, 121-131. https://doi.org/10.1016/j.bbr.2017.02.035

Arifiani Rosdiana, N., Dumarçay, S., Gérardin, C., Chapuis, H., Santiago-Me-dina, F.J., Kartika Sari, R., Syafii, W., Gelhaye, E., Raharivelomanana, P., Mohammed, R., & Gérardin, P. (2017). Characterization of bark extractives of different industrial Indonesian wood species for potential valorization. Industrial Crops & Products, 108, 121–127. https://doi.org/10.1016/j.indcrop.2017.06.034

Kebbi-Benkeder, Z., Manso, R., Gérardin, P., Dumarcay, S., Chopard, B. & Colin, F. (2017). Knot extractives: a model for analysing the eco-physiolo-gical factors that control the within and between-tree variability. Trees 31, 1619–1633. https://doi.org/10.1007/s00468-017-1573-z

31


Erikan Rizanti, D., Darmawan, W., George, B., Merlin, A., Dumarçay, S., Chapuis, H., Gérardin, C., Gelhaye, E., Raharivelomanana, P., Kartika Sari, R., Syafii, W., Mohamed R., & Gérardin, P. (2018). Comparison of teak wood properties according to forest management: Short versus long rotation. Annals of Forest Sciences, 75, 39. https://doi.org/10.1007/s13595-018-0716-8

Saha Tchinda, JB., Kor Ndikontar, M., Fouda Belinga, AD., Mounguengui, S., Njankouo, JM., Durmaçay, S., & Gérardin, P. (2018). Inhibition of fungi with wood extractives and natural durability of five Cameroonian wood species. Industrial Crops & Products, 123(1), 183–191. https://doi.org/10.1016/j.indcrop.2018.06.078

Schwartz, M., Perrot, T., Saiag, F., Aubert, E., Dumarçay, S., Gérardin, P., Mulliert, G., Morel-Rouhier, M., Favier, F., Didierjean, C., & Gelhaye, E. (2018). Molecular recognition of wood polyphenols by phase II detoxification enzymes of the white rot Trametes versicolor. Scientific Reports, 8, 8472. https://www.nature.com/articles/s41598-018-26601-3

Perrot, T., Schwartz, M., Saiag, F., Salzet, G., Dumarcay, S., Favier, F., Gérardin, P., Girardet, JM., Sormani, R., Morel-Rouhier, M., Amusant, N., Didierjean, C., & Gelhaye, E. (2018). Fungal glutathione transferases as tools to explore the chemical diversity of Amazonian wood extractives. ACS Sustainable Chemistry & Engineering, 6(10), 13078-13085. https://doi.org/10.1021/acssuschemeng.8b02636

Harlé, G., Kaminski, S., Dubayle, D., Frippiat, J.-P.*, & Ropars, A*. (2018). Murine splenic B cells express corticotropin-releasing hormone receptor 2 that affect their viability during a stress response. Scientific Reports, 9, 143. *Codirected this study. https://www.nature.com/articles/s41598-017-18401-y

D’Agostino, M., Lemmet, T., Dufay, C., Luc, A., Frippiat, J.-P., Machouart, M., & Debourgogne, A. (2018). Overinduction of CYP51A gene after exposure to azole antifungals provides a first clue to resistance mechanism in Fusarium solani species complex. Microbial Drug Resistance, 24(6), 768-773. https://doi.org/10.1089/mdr.2017.0311

Elmi, A., Spina, R., Abdoul-Latif, F., Yagi, S., Fontanay, S., Risler, A., Duval, R.E., & Laurain-Mattar, D. (2018). Rapid screening for bioactive natural compounds in Indigofera caerulea Rox Fruits. Industrial crops and products, 125(1), 123-130. https://doi.org/10.1016/j.indcrop.2018.08.089

Saha Tchinda, J.B., Kor Ndikontar, M., Fouda Belinga, A.D., Mounguengui, S., Njankouo, J.M., Durmaçay, S. & Gérardin, P. (2018). Inhibition of fungi with wood extractives and natural durability of five Cameroonian wood species. Industrial Crops & Products, 123, 183-191. https://doi.org/10.1016/j.indcrop.2018.06.078

Albe-Slabi, S., Mathé, C., Framboisier, X., Defaix, C., Mesieres, O., Galet, O., & Kapel, R. (2019). A new SE-HPLC method for simultaneous quantification of proteins and main phenolic compounds from sunflower meal aqueous extracts. Analytical and Bioanalytical Chemistry, 411, 2089-2099. https://doi.org/10.1007/s00216-019-01635-2

D’agostino, M., Tesse, N., Frippiat, J.-P., Machouart, M., & Debourgogne, A. (2019). Essential oils and their natural active compounds presenting antifungal properties. Molecules, 24(20), 3713. https://doi.org/10.3390/molecules24203713

Dubar, M., Frippiat, J.-P., Remen, T., Boufenzer, A., Alauzet, C., Baumann, C., Gibot, S., & Bisson C. (2020). Comparison of sTREM-1 and associated periodontal and bacterial factors before/after periodontal therapy, and impact of psychosocial factors. Journal of Clinical Periodontology, 47, 1064-1078. doi: 10.1111/jcpe.13339

Albe Slabi, S., Mathé, C., Basselin, M., Framboisier, X., Ndiaye, M., Galet, O., & Kapel, R. (2020). Multi-Objective Optimization of Solid/Liquid Extraction of Total Sunflower Proteins from Cold Press Meal. Food Chemistry 317, 126423. doi:10.1016/j.foodchem.2020.126423

Sara, A.S., Mathé, C., Basselin, M., Fournier, F., Aymes, A., Bianeis, M., Galet, O. & Kapel, R. (2020). Optimization of sunflower albumin extraction from oleaginous meal and characterization of their structure and properties. Food Hydrocolloids, 99, 105335. https://doi.org/10.1016/j.foodhyd.2019.105335

Brennan, M., Fritsch, C., Cosgun, S., Dumarcay, S., Colin, F., & Gérardin, P. (2020). Yield and compositions of bark phenolic extractives from three com-mercially significant softwoods show intra- and inter-specific variation. Plant Physiology and Biochemistry 155, 346–356. doi:10.32604/jrm.2021.013366

Chepkwony, S.C., Dumarçay, S., Chapuis, H., Kiprop, A., Gérardin, P. & Gé-rardin-Charbonnier, C. (2020). Geographic and intraspecific variability of mesquitol amounts in Prosopis juliflora trees from Kenya. European Journal of Wood and Wood Products, 78, 801-809. https://doi.org/10.1007/s00107-020-01535-8

Bopenga Bopenga, C.S.A., Dumarçay, S., Edou Engonga, P., & Gérardin, P. (2020). Relationships between chemical composition and decay durability of Coula edulis Baill as an alternative wood species in Gabon. Wood Science and Technology, 54, 329-348. https://doi.org/10.1007/s00226-020-01158-5

Brennan, M., Fritsch, C., Cosgun, S., Dumarcay, S., Colin, F. & Gérardin P. (2020). Quantitative and qualitative composition of bark polyphenols changes longitudinally with bark maturity in Abies alba Mill. Annals of Forest Science 77, 9. https://doi.org/10.1007/s13595-019-0916-x

Bruno, M.R., Russo, D., Cetera, P., Faraone, I., Lo Giudice, V., Milella, L., Todaro, L., Sinisgalli, C., Fritsch, C., Dumarçay S., & Gérardin P. (2020). Chemical analysis and antioxidant properties of orange tree (Citrus sinensis L.) biomass extracts obtained via different extraction techniques. Biofuels, Bioproducts & Biorefining 14, 509–520. https://doi.org/10.1002/bbb.2090

Brennan, M., Hentges, D. Cosgun, S., Dumarcay, S., Colin, F.s, Gérardin, C. & Gérardin, P. (2021). Intraspecific variability of quantity and chemical compo-sition of ethanolic knotwood extracts along the stems of three industrially important softwood species: Abies alba, Picea abies and Pseudotsuga menziesii. Holzforschung, 75, 168-179. https://doi.org/10.1515/hf-2020-0108

Bopenga Bopenga, C.S.A., Meyo Degboevi, H., Candelier, K., Edou Engonga, P., Dumarçay, S., Thévenon, M.F., Gérardin-Charbonnier, C., & Gérardin, C. (2021). Characterization of Extracts from the Bark of the Gabon Hazel Tree (Coula edulis baill) for Antioxidant, Antifungal and Anti-termite Products. Journal of Renewable Materials, 9, 17-33. doi: 10.32604/jrm.2020.013366

Austermann, K., Baecker N., Zwart, S.R., Fimmers, R., Frippiat, J.-P., Stehle, P., Smith S.M., & Heer M. (2021). Antioxidant supplementation did not affect bone turnover markers during 60 days of head-down tilt bed rest: Results from a randomized controlled trial. Journal of Nutrition, in press.

Patents

Humeau, C., Desobry, S., Chretien, F., & Oster, T. (2013). FR1354893

Desnoyers, L., & Frippiat, J.-P. (2016). Soleau envelope to protect the design of a system allowing the induction of accelerated ageing of bone structure and B lymphopoiesis in mice without surgical intervention, without phar-macological treatment and with minimal stress for the animals.

Galet, O., Kapel, R., & Albe Slabi, S. (2020). Sunflower Albumin Isolate and Process for the Production Thereof WO2020229430A1.

Galet, O., Kapel, R., & Albe Slabi, S. (2020). A sunflower seed protein isolate and a process for producing the same WO2020128051A1-1

31


Erikan Rizanti, D., Darmawan, W., George, B., Merlin, A., Dumarçay, S., Chapuis, H., Gérardin, C., Gelhaye, E., Raharivelomanana, P., Kartika Sari, R., Syafii, W., Mohamed R., & Gérardin, P. (2018). Comparison of teak wood properties according to forest management: Short versus long rotation. Annals of Forest Sciences, 75, 39. https://doi.org/10.1007/s13595-018-0716-8

Saha Tchinda, JB., Kor Ndikontar, M., Fouda Belinga, AD., Mounguengui, S., Njankouo, JM., Durmaçay, S., & Gérardin, P. (2018). Inhibition of fungi with wood extractives and natural durability of five Cameroonian wood species. Industrial Crops & Products, 123(1), 183–191. https://doi.org/10.1016/j.indcrop.2018.06.078

Schwartz, M., Perrot, T., Saiag, F., Aubert, E., Dumarçay, S., Gérardin, P., Mulliert, G., Morel-Rouhier, M., Favier, F., Didierjean, C., & Gelhaye, E. (2018). Molecular recognition of wood polyphenols by phase II detoxification enzymes of the white rot Trametes versicolor. Scientific Reports, 8, 8472. https://www.nature.com/articles/s41598-018-26601-3

Perrot, T., Schwartz, M., Saiag, F., Salzet, G., Dumarcay, S., Favier, F., Gérardin, P., Girardet, JM., Sormani, R., Morel-Rouhier, M., Amusant, N., Didierjean, C., & Gelhaye, E. (2018). Fungal glutathione transferases as tools to explore the chemical diversity of Amazonian wood extractives. ACS Sustainable Chemistry & Engineering, 6(10), 13078-13085. https://doi.org/10.1021/acssuschemeng.8b02636

Harlé, G., Kaminski, S., Dubayle, D., Frippiat, J.-P.*, & Ropars, A*. (2018). Murine splenic B cells express corticotropin-releasing hormone receptor 2 that affect their viability during a stress response. Scientific Reports, 9, 143. *Codirected this study. https://www.nature.com/articles/s41598-017-18401-y

D’Agostino, M., Lemmet, T., Dufay, C., Luc, A., Frippiat, J.-P., Machouart, M., & Debourgogne, A. (2018). Overinduction of CYP51A gene after exposure to azole antifungals provides a first clue to resistance mechanism in Fusarium solani species complex. Microbial Drug Resistance, 24(6), 768-773. https://doi.org/10.1089/mdr.2017.0311

Elmi, A., Spina, R., Abdoul-Latif, F., Yagi, S., Fontanay, S., Risler, A., Duval, R.E., & Laurain-Mattar, D. (2018). Rapid screening for bioactive natural compounds in Indigofera caerulea Rox Fruits. Industrial crops and products, 125(1), 123-130. https://doi.org/10.1016/j.indcrop.2018.08.089

Saha Tchinda, J.B., Kor Ndikontar, M., Fouda Belinga, A.D., Mounguengui, S., Njankouo, J.M., Durmaçay, S. & Gérardin, P. (2018). Inhibition of fungi with wood extractives and natural durability of five Cameroonian wood species. Industrial Crops & Products, 123, 183-191. https://doi.org/10.1016/j.indcrop.2018.06.078

Albe-Slabi, S., Mathé, C., Framboisier, X., Defaix, C., Mesieres, O., Galet, O., & Kapel, R. (2019). A new SE-HPLC method for simultaneous quantification of proteins and main phenolic compounds from sunflower meal aqueous extracts. Analytical and Bioanalytical Chemistry, 411, 2089-2099. https://doi.org/10.1007/s00216-019-01635-2

D’agostino, M., Tesse, N., Frippiat, J.-P., Machouart, M., & Debourgogne, A. (2019). Essential oils and their natural active compounds presenting antifungal properties. Molecules, 24(20), 3713. https://doi.org/10.3390/molecules24203713

Dubar, M., Frippiat, J.-P., Remen, T., Boufenzer, A., Alauzet, C., Baumann, C., Gibot, S., & Bisson C. (2020). Comparison of sTREM-1 and associated periodontal and bacterial factors before/after periodontal therapy, and impact of psychosocial factors. Journal of Clinical Periodontology, 47, 1064-1078. doi: 10.1111/jcpe.13339

Albe Slabi, S., Mathé, C., Basselin, M., Framboisier, X., Ndiaye, M., Galet, O., & Kapel, R. (2020). Multi-Objective Optimization of Solid/Liquid Extraction of Total Sunflower Proteins from Cold Press Meal. Food Chemistry 317, 126423. doi:10.1016/j.foodchem.2020.126423

Sara, A.S., Mathé, C., Basselin, M., Fournier, F., Aymes, A., Bianeis, M., Galet, O. & Kapel, R. (2020). Optimization of sunflower albumin extraction from oleaginous meal and characterization of their structure and properties. Food Hydrocolloids, 99, 105335. https://doi.org/10.1016/j.foodhyd.2019.105335

Brennan, M., Fritsch, C., Cosgun, S., Dumarcay, S., Colin, F., & Gérardin, P. (2020). Yield and compositions of bark phenolic extractives from three com-mercially significant softwoods show intra- and inter-specific variation. Plant Physiology and Biochemistry 155, 346–356. doi:10.32604/jrm.2021.013366

Chepkwony, S.C., Dumarçay, S., Chapuis, H., Kiprop, A., Gérardin, P. & Gé-rardin-Charbonnier, C. (2020). Geographic and intraspecific variability of mesquitol amounts in Prosopis juliflora trees from Kenya. European Journal of Wood and Wood Products, 78, 801-809. https://doi.org/10.1007/s00107-020-01535-8

Bopenga Bopenga, C.S.A., Dumarçay, S., Edou Engonga, P., & Gérardin, P. (2020). Relationships between chemical composition and decay durability of Coula edulis Baill as an alternative wood species in Gabon. Wood Science and Technology, 54, 329-348. https://doi.org/10.1007/s00226-020-01158-5

Brennan, M., Fritsch, C., Cosgun, S., Dumarcay, S., Colin, F. & Gérardin P. (2020). Quantitative and qualitative composition of bark polyphenols changes longitudinally with bark maturity in Abies alba Mill. Annals of Forest Science 77, 9. https://doi.org/10.1007/s13595-019-0916-x

Bruno, M.R., Russo, D., Cetera, P., Faraone, I., Lo Giudice, V., Milella, L., Todaro, L., Sinisgalli, C., Fritsch, C., Dumarçay S., & Gérardin P. (2020). Chemical analysis and antioxidant properties of orange tree (Citrus sinensis L.) biomass extracts obtained via different extraction techniques. Biofuels, Bioproducts & Biorefining 14, 509–520. https://doi.org/10.1002/bbb.2090

Brennan, M., Hentges, D. Cosgun, S., Dumarcay, S., Colin, F.s, Gérardin, C. & Gérardin, P. (2021). Intraspecific variability of quantity and chemical compo-sition of ethanolic knotwood extracts along the stems of three industrially important softwood species: Abies alba, Picea abies and Pseudotsuga menziesii. Holzforschung, 75, 168-179. https://doi.org/10.1515/hf-2020-0108

Bopenga Bopenga, C.S.A., Meyo Degboevi, H., Candelier, K., Edou Engonga, P., Dumarçay, S., Thévenon, M.F., Gérardin-Charbonnier, C., & Gérardin, C. (2021). Characterization of Extracts from the Bark of the Gabon Hazel Tree (Coula edulis baill) for Antioxidant, Antifungal and Anti-termite Products. Journal of Renewable Materials, 9, 17-33. doi: 10.32604/jrm.2020.013366

Austermann, K., Baecker N., Zwart, S.R., Fimmers, R., Frippiat, J.-P., Stehle, P., Smith S.M., & Heer M. (2021). Antioxidant supplementation did not affect bone turnover markers during 60 days of head-down tilt bed rest: Results from a randomized controlled trial. Journal of Nutrition, in press.

Patents

Humeau, C., Desobry, S., Chretien, F., & Oster, T. (2013). FR1354893

Desnoyers, L., & Frippiat, J.-P. (2016). Soleau envelope to protect the design of a system allowing the induction of accelerated ageing of bone structure and B lymphopoiesis in mice without surgical intervention, without phar-macological treatment and with minimal stress for the animals.

Galet, O., Kapel, R., & Albe Slabi, S. (2020). Sunflower Albumin Isolate and Process for the Production Thereof WO2020229430A1.

Galet, O., Kapel, R., & Albe Slabi, S. (2020). A sunflower seed protein isolate and a process for producing the same WO2020128051A1-1

32

Informatics products and toolsDatabase

Spina, R., Dupire, F., Laurain-Mattar, D., DataNat, database dedicated to the identification of naturally occurring molecules by mass spectrometry. IDDN number: FR.001.480019.000.S.P.2020.000.10300.

CommunicationOral presentations

Laurain-Mattar, D. A rapid identification of plants natural compounds by HPTLC-MS approach. Journée Scientifique du Club de CCM, November 8, 2018 - Strasbourg (France)

Fonte, C., Kaminski, S., Vanet, A., Ghislin, S., & Frippiat, J.-P. Socio-environ-mental stressors encountered during spaceflight partially affect the murine TCR repertoire and increase its self-reactivity. 40th Annual Meeting of the International Society for Gravitational Physiology and Space Life Science and Medicine Meeting, May 26-31, 2019 - Nagoya (Japan)

Tascher, G., Gerbaix, M., Ghislin, S., Antropova, E., Vassilieva, G., Vico, L., Frippiat, J.-P., & Bertile, F. Analysis of femurs from mice embar-ked on board BION-M1 biosatellite reveals a decrease in immune cell development, including B cells, after one week of recovery on Earth. 40th Annual Meeting of the International Society for Gravitational Physiology and Space Life Science and Medicine Meeting, May 26-31, 2019 - Nagoya (Japan)

Le, T.T., Ioannou, I., Ropars, A., Aymes, A., Frippiat, J.-P., & Kapel, R. Optimization of conditions for the purification of chlorogenic acid from a sunflower meal co-product by macroporous resins: static and dynamic study. 12th European Congress of Chemical Engineering, September 15-19, 2019 - Florence (Italy)

Frippiat, J.-P. Effect of bed rest on the creation of B lymphocyte antigen receptor repertoire and evaluation of the benefits of a nutritional supple-mentation with anti-inflammatory and anti-oxidant substances. Invited by Prof. D. Thieme. March 25, 2019 - Dresden (Germany)

Frippiat, J.-P. The B-cell receptor under simulated microgravity conditions, an example of accelerated aging? 40th Annual Meeting of the International Society for Gravitational Physiology and Space Life Science and Medicine Meeting, May 26-31, 2019 – Nagoya (Japan)

Posters

Le, T.T., Ioannou, I., Ropars, A., Aymes, A., Frippiat, J.-P., & Kapel, R. Optimi-zation of chlorogenic acid purification from sunflower meal by macroporous resins: static/dynamic study. Congress of American Oil Chemists’ Society, May 5-8, 2019 - St. Louis, Missouri (USA).

D’Agostino, M., Tesse, N., Frippiat, J.-P., Machouart, M., & Debour-gogne, A. In vitro antifungal effect of plant-based compound CIN-102 on filamentous fungi. 9th Trends in Medical Mycology meeting, October 11-14, 2019 – Nice (France).

Colin, F. Une double contribution pour la chimie du bois et la bioéconomie régionale : les projets régionaux ExtraFor_Est et Gemm_Est. GDR Bois 18-20 Novembre 2019 ENSTIB Epinal (France).

Le, T.T., Ropars, A., Aymes, A., Frippiat, J.-P., & Kapel, R. Optimizing the purification of chlorogenic acid from protein by-product of sunflower meal and their bioactive functions. Congress of GreenFoodTech (GFT) 2020, May 5 and 6, 2020, Quebec City, Quebec, Canada.

Ribeiro S., Colin F., Jolivet Y., Vaultier MN., Contini A., Gérardin P., Dumarçay S. & Leneveu L. Projet Gemm_Est : le retour du gemmage dans le Grand Est. 9èmes journées du GDR 3544 «Sciences du bois», 18-20 novembre 2020, Grenoble (France).

Nekkaa, A., Benaissa, A., Mutelet, F., & Canabady-Rochelle, L. Green extrac-tion processes for high-value bioactive compounds from medicinal plants: theory and practice. Plant sciences & research, 10-11 May 2021.

Nekkaa, A., Benaissa, A., Mutelet, F., & Canabady-Rochelle, L. Phytochemical and Pharmacological Properties of an Algerian Medicinal Plant Extract’s (Rhamnus alaternus): A review. international conference for Functional Food Center (FFC). 29 – 30 Avril 2021, Dallas, TX, USA.

Transversal Action 3: Metal chelating peptidesPublications

Csire, G., Canabady-Rochelle, L., Averlant-Petit, M.-C., Selmeczi, K., & Ste-fan L. (2020). Both metal-chelating and free radical-scavenging synthetic pentapeptides as efficient inhibitors of reactive oxygen species generation. Metallomics, 12, 1220-1229. https://doi.org/10.1039/d0mt00103a

El-Hayn R., Canabady-Rochelle L., Desmarets C., Balan L., Rinnert H., Joubert O., Medjahdi G., Ben Ouada H., Schneider R. One-step synthesis of diamine-functionalized graphene quantum dots from graphene oxide and their chelating and antioxidant activities. Nanoparticules, 2020, 10, 104. https://doi.org/10.3390/nano10010104

Gaucher, C., Boudier, A., Bonetti, J., Clarot, I., Leroy, P., & Parent, M. (2018). Glutathione: antioxidant properties dedicated to nanotechnologies. An-tioxidants 7, pii: E62. https://doi.org/10.3390/antiox7050062

Muhr L, Pontvianne S., Selmeczi, K., Paris C., Boschi-Muller S. and Cana-bady-Rochelle L. Chromatographic Separation Simulation of Metal-Chelating Peptides from Surface Plasmon Resonance Binding Parameters. Journal of Separation Science. http://doi.wiley.com/10.1002/jssc.201900882

Pavel, I. -A., Prazeres, S. F., Montalvo, G., Garcia Ruiz, C., Nicolas, V., Celzard, A., Dehez, F., Canabady-Rochelle, L., Canilho, N., & Pasc, A. (2017). Effect of meso vs macro-size of hierarchical porous silica on the adsorption and activity of immobilized β-galactosidase. Langmuir, 33, 3333-3340. https://doi.org/10.1021/acs.langmuir.7b00134

Paris C., Selmeczi, K., Ebel, B., Stefan, L., Csire, G., Cakir-Kiefer, C., Desobry, S., Canabady-Rochelle, L., and Chaimbault, P. Metabolomic approach based on LC-HRMS for the fast screening of iron-(II)-chelating peptides in protein hydrolysates. Analytical and Bioanalytical Chemistry (IF:3.7). Accepted. https://rdcu.be/ccYI4Publications whose topic is related to TA3Akingbogun, O., Muhr, L., & Vallières, C. (2017). Capture of carbon dioxide by an ion exchange resin: Influence of gas humidity on capture mechanisms. Separation Science and Technology, 52, 1761-1767. https://doi.org/10.1080/01496395.2017.1293094

Benoit, S., Chaumontet, C., Schwarz, J., Cakir-Kiefer, C., Tomé, D., & Miclo, L. (2017). Mapping in mice the brain regions involved in the anxiolytic-like properties of alpha-cazozepine, a tryptic peptide derived from bovin alpha s1-casein. Journal of Functional Foods, 38, 464-473. https://doi.org/10.1016/j.jff.2017.09.014

Canabady-Rochelle, L., Selmeczi, K., Collin, S., Pacs, A., Muhr, L. & Boschi-Mul-ler, S. (2018). SPR Screening of Metal chelating Peptides for their Antioxidant Properties. Food Chemistry, 239, 478-485. https://doi.org/10.1016/j.foodchem.2017.06.116

32

Informatics products and toolsDatabase

Spina, R., Dupire, F., Laurain-Mattar, D., DataNat, database dedicated to the identification of naturally occurring molecules by mass spectrometry. IDDN number: FR.001.480019.000.S.P.2020.000.10300.

CommunicationOral presentations

Laurain-Mattar, D. A rapid identification of plants natural compounds by HPTLC-MS approach. Journée Scientifique du Club de CCM, November 8, 2018 - Strasbourg (France)

Fonte, C., Kaminski, S., Vanet, A., Ghislin, S., & Frippiat, J.-P. Socio-environ-mental stressors encountered during spaceflight partially affect the murine TCR repertoire and increase its self-reactivity. 40th Annual Meeting of the International Society for Gravitational Physiology and Space Life Science and Medicine Meeting, May 26-31, 2019 - Nagoya (Japan)

Tascher, G., Gerbaix, M., Ghislin, S., Antropova, E., Vassilieva, G., Vico, L., Frippiat, J.-P., & Bertile, F. Analysis of femurs from mice embar-ked on board BION-M1 biosatellite reveals a decrease in immune cell development, including B cells, after one week of recovery on Earth. 40th Annual Meeting of the International Society for Gravitational Physiology and Space Life Science and Medicine Meeting, May 26-31, 2019 - Nagoya (Japan)

Le, T.T., Ioannou, I., Ropars, A., Aymes, A., Frippiat, J.-P., & Kapel, R. Optimization of conditions for the purification of chlorogenic acid from a sunflower meal co-product by macroporous resins: static and dynamic study. 12th European Congress of Chemical Engineering, September 15-19, 2019 - Florence (Italy)

Frippiat, J.-P. Effect of bed rest on the creation of B lymphocyte antigen receptor repertoire and evaluation of the benefits of a nutritional supple-mentation with anti-inflammatory and anti-oxidant substances. Invited by Prof. D. Thieme. March 25, 2019 - Dresden (Germany)

Frippiat, J.-P. The B-cell receptor under simulated microgravity conditions, an example of accelerated aging? 40th Annual Meeting of the International Society for Gravitational Physiology and Space Life Science and Medicine Meeting, May 26-31, 2019 – Nagoya (Japan)

Posters

Le, T.T., Ioannou, I., Ropars, A., Aymes, A., Frippiat, J.-P., & Kapel, R. Optimi-zation of chlorogenic acid purification from sunflower meal by macroporous resins: static/dynamic study. Congress of American Oil Chemists’ Society, May 5-8, 2019 - St. Louis, Missouri (USA).

D’Agostino, M., Tesse, N., Frippiat, J.-P., Machouart, M., & Debour-gogne, A. In vitro antifungal effect of plant-based compound CIN-102 on filamentous fungi. 9th Trends in Medical Mycology meeting, October 11-14, 2019 – Nice (France).

Colin, F. Une double contribution pour la chimie du bois et la bioéconomie régionale : les projets régionaux ExtraFor_Est et Gemm_Est. GDR Bois 18-20 Novembre 2019 ENSTIB Epinal (France).

Le, T.T., Ropars, A., Aymes, A., Frippiat, J.-P., & Kapel, R. Optimizing the purification of chlorogenic acid from protein by-product of sunflower meal and their bioactive functions. Congress of GreenFoodTech (GFT) 2020, May 5 and 6, 2020, Quebec City, Quebec, Canada.

Ribeiro S., Colin F., Jolivet Y., Vaultier MN., Contini A., Gérardin P., Dumarçay S. & Leneveu L. Projet Gemm_Est : le retour du gemmage dans le Grand Est. 9èmes journées du GDR 3544 «Sciences du bois», 18-20 novembre 2020, Grenoble (France).

Nekkaa, A., Benaissa, A., Mutelet, F., & Canabady-Rochelle, L. Green extrac-tion processes for high-value bioactive compounds from medicinal plants: theory and practice. Plant sciences & research, 10-11 May 2021.

Nekkaa, A., Benaissa, A., Mutelet, F., & Canabady-Rochelle, L. Phytochemical and Pharmacological Properties of an Algerian Medicinal Plant Extract’s (Rhamnus alaternus): A review. international conference for Functional Food Center (FFC). 29 – 30 Avril 2021, Dallas, TX, USA.

Transversal Action 3: Metal chelating peptidesPublications

Csire, G., Canabady-Rochelle, L., Averlant-Petit, M.-C., Selmeczi, K., & Ste-fan L. (2020). Both metal-chelating and free radical-scavenging synthetic pentapeptides as efficient inhibitors of reactive oxygen species generation. Metallomics, 12, 1220-1229. https://doi.org/10.1039/d0mt00103a

El-Hayn R., Canabady-Rochelle L., Desmarets C., Balan L., Rinnert H., Joubert O., Medjahdi G., Ben Ouada H., Schneider R. One-step synthesis of diamine-functionalized graphene quantum dots from graphene oxide and their chelating and antioxidant activities. Nanoparticules, 2020, 10, 104. https://doi.org/10.3390/nano10010104

Gaucher, C., Boudier, A., Bonetti, J., Clarot, I., Leroy, P., & Parent, M. (2018). Glutathione: antioxidant properties dedicated to nanotechnologies. An-tioxidants 7, pii: E62. https://doi.org/10.3390/antiox7050062

Muhr L, Pontvianne S., Selmeczi, K., Paris C., Boschi-Muller S. and Cana-bady-Rochelle L. Chromatographic Separation Simulation of Metal-Chelating Peptides from Surface Plasmon Resonance Binding Parameters. Journal of Separation Science. http://doi.wiley.com/10.1002/jssc.201900882

Pavel, I. -A., Prazeres, S. F., Montalvo, G., Garcia Ruiz, C., Nicolas, V., Celzard, A., Dehez, F., Canabady-Rochelle, L., Canilho, N., & Pasc, A. (2017). Effect of meso vs macro-size of hierarchical porous silica on the adsorption and activity of immobilized β-galactosidase. Langmuir, 33, 3333-3340. https://doi.org/10.1021/acs.langmuir.7b00134

Paris C., Selmeczi, K., Ebel, B., Stefan, L., Csire, G., Cakir-Kiefer, C., Desobry, S., Canabady-Rochelle, L., and Chaimbault, P. Metabolomic approach based on LC-HRMS for the fast screening of iron-(II)-chelating peptides in protein hydrolysates. Analytical and Bioanalytical Chemistry (IF:3.7). Accepted. https://rdcu.be/ccYI4Publications whose topic is related to TA3Akingbogun, O., Muhr, L., & Vallières, C. (2017). Capture of carbon dioxide by an ion exchange resin: Influence of gas humidity on capture mechanisms. Separation Science and Technology, 52, 1761-1767. https://doi.org/10.1080/01496395.2017.1293094

Benoit, S., Chaumontet, C., Schwarz, J., Cakir-Kiefer, C., Tomé, D., & Miclo, L. (2017). Mapping in mice the brain regions involved in the anxiolytic-like properties of alpha-cazozepine, a tryptic peptide derived from bovin alpha s1-casein. Journal of Functional Foods, 38, 464-473. https://doi.org/10.1016/j.jff.2017.09.014

Canabady-Rochelle, L., Selmeczi, K., Collin, S., Pacs, A., Muhr, L. & Boschi-Mul-ler, S. (2018). SPR Screening of Metal chelating Peptides for their Antioxidant Properties. Food Chemistry, 239, 478-485. https://doi.org/10.1016/j.foodchem.2017.06.116

33


Chour, Z., Laubie, B., Morel, J. L., Tang, Y., Qiu, R., Simonnot, M. O., & Muhr, L. (2018). Recovery of Rare Earth Elements from Dicranopteris dichotoma by an enhanced ion exchange leaching process. Chemical Engineering and Processing, 130, 208-213. https://doi.org/10.1016/j.cep.2018.06.007

Dancs, Á., Selmeczi, K., Nagy, N. V., & Gajda, T. (2018). On the copper(II) binding of asymmetrically functionalized tripodal peptides: solution equi-librium, structure, and enzyme mimicking. New Journal of Chemistry, 42, 7746-7757. DOI: 10.1039/C7NJ04716A

Dancs, Á., Selmeczi, K., Bányai, I., Darula, Z., & Gajda, T. (2018). Increasing the histidine ‘density’ in tripodal peptides by gradual N-functionaliza-tion of tris(2-aminoethyl)amine (tren) with L-histidyl units: the effect on zinc(II) complexes. Inorganica Chemica Acta, 472, 174-183. DOI:10.1016/j.ica.2017.06.049

De Paula, Q. A., Joly, J. -P., Selmeczi, K., Fonseca, D. E. P., Caramori, G. F., Farrell, N. P., & Da Costa Ferreira, A.M. (2018). Binding affinity studies of 1,2,3-triazole copper(II) complexes to human serum albumin. Journal of Coordination Chemistry, 71, 1894-1909. https://doi.org/10.1080/00958972.2018.1495331

Ferjaoui, Z., Schneider, R., Meftah, A., Gaffet, E., & Alem, H. (2017). Functional responsive superparamagnetic Core/Shell nanoparticles and their drug release properties. RSC Advances, 7, 26243–26249. https://doi.org/10.1039/C7RA02437A

Haffner, F. B., & Pasc, A. (2018). Freeze-dried alginate-silica microparticles as carriers of probiotic bacteria in apple juice and beer. LWT - Food Science and Technology, 91, 175-179. DOI: 10.1016/j.lwt.2018.01.050

Hilbold, N. J., Le Saoût, X., Valery, E., & Muhr, L., Souquet, J., Lamproye, A., Broly, H. (2017). Evaluation of several protein A resins for application to multi‐column chromatography for the rapid purification of fed‐batch bioreactors. Biotechnology Progress, 33(4), 941-953. Doi: 10.1002/btpr.2465. Epub 2017 Apr 26.

Jaoudi, M., Ding, J., Hannachi, A., & Muhr, L. IEX and BMED hybrid process for dilute organic acids recovery: identification of key steps to manage energy consumption. (2017). Desalination and Water Treament, 69, 123-129. DOI: 10.5004/dwt.2017.11373

Michalska, M., Florczak, A., Dams-Kozlowska, H., Gapinski, J., Jurga, S., & Schneider, R. (2016). Peptide-functionalized ZCIS QDs as fluorescent nanoprobe for targeted HER2-positive breast cancer cells imaging. Acta Biomateralia, 35, 293-304. DOI: 10.1016/j.actbio.2016.02.002

Pavel, I. -A., Girardon, M., El Hajj, S., Parant, S., Amadei, F., Kaufmann, S., Tanaka, M., Fierro, V., Celzard, A., Canilho, N., & Pasc, A. (2018). Lipid-coated mesoporous silica microparticles for the controlled delivery of β-galac-tosidase into intestines. Journal of Material Chemistry B, 6, 5633-5639. https://doi.org/10.1039/C8TB01114A

Prazeres, S. F., Zapata, F., Canilho, N., Pasc, A., García-Ruiz, C., & Montalvo, G. (2019). Probing the confinement of β-galactosidase into meso-macro porous silica by Raman spectroscopy. Microporous and Mesoporous Materials, 278, 149–155. https://doi.org/10.1016/j.micromeso.2018.11.032

Przysiecka, L., Michalska, M., Nowaczyk, G., Peplinska, B., Jesionowski, T., Schneider, R., & Jurga, S. (2016). iRGD peptide as effective transporter of CuInZnxS2+x quantum dots into human cancer cells. Colloid Surface B, 146, 9-18. DOI: 10.1016/j.colsurfb.2016.05.041

Rammal, H., Dubus, M., Aubert, L., Reffuveille, F., Laurent-Maquin, D., Terryn, C., Schaaf, P., Alem, H., Francius, G., Quilès, F. et al. (2017). Bioinspired nanofeatured substrates: suitable environment for bone regeneration. ACS Applied Materials & Interfaces, 9(14), 12791–12801. https://doi.org/10.1021/acsami.7b01665

Ravetti Duran, R., Escudero Falsetti, P., Muhr, L., Privat, R., & Barth, D. (2018). Phase equilibrium study of the ternary system CO2+H2O+Ethanol at elevated pressure: thermodynamic model selection. Application to supercritical extraction of polar compounds. The Journal of Supercritical Fluid, 138, 17-28. https://doi.org/10.1016/j.supflu.2018.03.016

Publications submittedCsire, G., Dupire, F. Canabady-Rochelle, L., Selmeczi, K., Stefan, L. Bio-ins-pired casein-derived antioxidant peptides exhibiting a dual direct/indirect mode of action. Inorganic Chemistry. Submitted.

Djehel, T., Cakir-Kiefer, C., Reti, B., Belaissaoui, B., Roizard, D., Pasc, A., Celzard, A., & Canabady-Rochelle, L. Hydrolysis of soy protein isolate for the production of antioxidant peptides involving various chemical mechanisms. Journal of Food Science. Under review.

El hajj, S., Sepulveda-Rincon, T., Girardet, J-M., Cakir-Kiefer, C., Stefan, L., Zapata Montoya, J-E., Boschi-Muller, S., Gaucher, C., & Canabady-Rochelle, L. Electrically switchable nanolever technology for the screening of me-tal-chelating peptides in hydrolysate. Journal of Agricultural & Food che-mistry. In submission.

Book chaptersEl Hajj, S., Sepulveda-Rincon, T., Paris, C., Giraud, T., Csire, G., Stefan, L., Selmeczi, K., Girardet, J.-M., Désobry, S., Bouhallab, S., Muhr, L., Gaucher, C., Canabady-Rochelle (2021) ‘Application in Nutrition: Mineral-Binding’ in Toldra, F., & Wu, J. (ed.) Biologically active peptides. Chapitre 19 accepté. Publication en juin 2021. https://www.elsevier.com/books/biologically-ac-tive-peptides/toldra/978-0-12-821389-6

Stefan L. (2017). Amino Acid - New Insights and Roles in Plant and Animal (Ed. T. Asao and Md. Asaduzzaman) INTECH, (ISBN 978-953-51-3242-4). ‘Amino acids modifications to improve and fine-tune peptide-based hydrogels’. DOI: 10.5772/intechopen.68705


Canabady-Rochelle. Presentation of the MELISSA project. ANR Kick-off meeting, 11 December 2020.

Csire, G. Averlant-Petit, M.-C., Stefan, L., & Selmeczi, K. (2020, October). Iron(III)-chelating model peptides as bioinspired antioxidants. Oral session presentation at the Conférence FrenchBIC - Réunion annuelle du Groupe Français de Chimie Bio-Inorganique, online conference (France).

Csire G., Paris C., Canabady-Rochelle L., Stefan L., Selmeczi K. Iron(III)-che-lating model peptides as bioinspired antioxidants. LUE International Confe-rence “Biomolecules: Research & Development, Markets and Acceptability”. 23th-25th October 2019, Palma de Majorca, Spain. Flash presentation flash.

El Hajj, S., Canabady-Rochelle, L., Irankunda, R., Fries, I. & Gaucher, C. Investigating the antioxidant activity of metal-chelating peptides in soy hydrolysates on a cell-based ferroptosis model. 29th international conference for Functional Food Center (FFC). 29 – 30 Avril 2021, Dallas, TX, État-Unis. Oral presentation. Online conference.

El Hajj, S., Cakir-Kiefer, C., Stefan, L., Pontvianne, S., Arnoux, P., Paris, C., Muhr, L., Selmeczi, K., Desobry, S., Boschi-Muller, S., Gaucher, C., Cana-bady-Rochelle, L. Metal-chelating peptides in hydrolysates: biocatalysis production, characterization and screening. Seminar IMPACT BIOMOLECULE 2019. 23-25 Octobre, Palma de Mallorca, Espagne. Flash presentation.

33


Chour, Z., Laubie, B., Morel, J. L., Tang, Y., Qiu, R., Simonnot, M. O., & Muhr, L. (2018). Recovery of Rare Earth Elements from Dicranopteris dichotoma by an enhanced ion exchange leaching process. Chemical Engineering and Processing, 130, 208-213. https://doi.org/10.1016/j.cep.2018.06.007

Dancs, Á., Selmeczi, K., Nagy, N. V., & Gajda, T. (2018). On the copper(II) binding of asymmetrically functionalized tripodal peptides: solution equi-librium, structure, and enzyme mimicking. New Journal of Chemistry, 42, 7746-7757. DOI: 10.1039/C7NJ04716A

Dancs, Á., Selmeczi, K., Bányai, I., Darula, Z., & Gajda, T. (2018). Increasing the histidine ‘density’ in tripodal peptides by gradual N-functionaliza-tion of tris(2-aminoethyl)amine (tren) with L-histidyl units: the effect on zinc(II) complexes. Inorganica Chemica Acta, 472, 174-183. DOI:10.1016/j.ica.2017.06.049

De Paula, Q. A., Joly, J. -P., Selmeczi, K., Fonseca, D. E. P., Caramori, G. F., Farrell, N. P., & Da Costa Ferreira, A.M. (2018). Binding affinity studies of 1,2,3-triazole copper(II) complexes to human serum albumin. Journal of Coordination Chemistry, 71, 1894-1909. https://doi.org/10.1080/00958972.2018.1495331

Ferjaoui, Z., Schneider, R., Meftah, A., Gaffet, E., & Alem, H. (2017). Functional responsive superparamagnetic Core/Shell nanoparticles and their drug release properties. RSC Advances, 7, 26243–26249. https://doi.org/10.1039/C7RA02437A

Haffner, F. B., & Pasc, A. (2018). Freeze-dried alginate-silica microparticles as carriers of probiotic bacteria in apple juice and beer. LWT - Food Science and Technology, 91, 175-179. DOI: 10.1016/j.lwt.2018.01.050

Hilbold, N. J., Le Saoût, X., Valery, E., & Muhr, L., Souquet, J., Lamproye, A., Broly, H. (2017). Evaluation of several protein A resins for application to multi‐column chromatography for the rapid purification of fed‐batch bioreactors. Biotechnology Progress, 33(4), 941-953. Doi: 10.1002/btpr.2465. Epub 2017 Apr 26.

Jaoudi, M., Ding, J., Hannachi, A., & Muhr, L. IEX and BMED hybrid process for dilute organic acids recovery: identification of key steps to manage energy consumption. (2017). Desalination and Water Treament, 69, 123-129. DOI: 10.5004/dwt.2017.11373

Michalska, M., Florczak, A., Dams-Kozlowska, H., Gapinski, J., Jurga, S., & Schneider, R. (2016). Peptide-functionalized ZCIS QDs as fluorescent nanoprobe for targeted HER2-positive breast cancer cells imaging. Acta Biomateralia, 35, 293-304. DOI: 10.1016/j.actbio.2016.02.002

Pavel, I. -A., Girardon, M., El Hajj, S., Parant, S., Amadei, F., Kaufmann, S., Tanaka, M., Fierro, V., Celzard, A., Canilho, N., & Pasc, A. (2018). Lipid-coated mesoporous silica microparticles for the controlled delivery of β-galac-tosidase into intestines. Journal of Material Chemistry B, 6, 5633-5639. https://doi.org/10.1039/C8TB01114A

Prazeres, S. F., Zapata, F., Canilho, N., Pasc, A., García-Ruiz, C., & Montalvo, G. (2019). Probing the confinement of β-galactosidase into meso-macro porous silica by Raman spectroscopy. Microporous and Mesoporous Materials, 278, 149–155. https://doi.org/10.1016/j.micromeso.2018.11.032

Przysiecka, L., Michalska, M., Nowaczyk, G., Peplinska, B., Jesionowski, T., Schneider, R., & Jurga, S. (2016). iRGD peptide as effective transporter of CuInZnxS2+x quantum dots into human cancer cells. Colloid Surface B, 146, 9-18. DOI: 10.1016/j.colsurfb.2016.05.041

Rammal, H., Dubus, M., Aubert, L., Reffuveille, F., Laurent-Maquin, D., Terryn, C., Schaaf, P., Alem, H., Francius, G., Quilès, F. et al. (2017). Bioinspired nanofeatured substrates: suitable environment for bone regeneration. ACS Applied Materials & Interfaces, 9(14), 12791–12801. https://doi.org/10.1021/acsami.7b01665

Ravetti Duran, R., Escudero Falsetti, P., Muhr, L., Privat, R., & Barth, D. (2018). Phase equilibrium study of the ternary system CO2+H2O+Ethanol at elevated pressure: thermodynamic model selection. Application to supercritical extraction of polar compounds. The Journal of Supercritical Fluid, 138, 17-28. https://doi.org/10.1016/j.supflu.2018.03.016

Publications submittedCsire, G., Dupire, F. Canabady-Rochelle, L., Selmeczi, K., Stefan, L. Bio-ins-pired casein-derived antioxidant peptides exhibiting a dual direct/indirect mode of action. Inorganic Chemistry. Submitted.

Djehel, T., Cakir-Kiefer, C., Reti, B., Belaissaoui, B., Roizard, D., Pasc, A., Celzard, A., & Canabady-Rochelle, L. Hydrolysis of soy protein isolate for the production of antioxidant peptides involving various chemical mechanisms. Journal of Food Science. Under review.

El hajj, S., Sepulveda-Rincon, T., Girardet, J-M., Cakir-Kiefer, C., Stefan, L., Zapata Montoya, J-E., Boschi-Muller, S., Gaucher, C., & Canabady-Rochelle, L. Electrically switchable nanolever technology for the screening of me-tal-chelating peptides in hydrolysate. Journal of Agricultural & Food che-mistry. In submission.

Book chaptersEl Hajj, S., Sepulveda-Rincon, T., Paris, C., Giraud, T., Csire, G., Stefan, L., Selmeczi, K., Girardet, J.-M., Désobry, S., Bouhallab, S., Muhr, L., Gaucher, C., Canabady-Rochelle (2021) ‘Application in Nutrition: Mineral-Binding’ in Toldra, F., & Wu, J. (ed.) Biologically active peptides. Chapitre 19 accepté. Publication en juin 2021. https://www.elsevier.com/books/biologically-ac-tive-peptides/toldra/978-0-12-821389-6

Stefan L. (2017). Amino Acid - New Insights and Roles in Plant and Animal (Ed. T. Asao and Md. Asaduzzaman) INTECH, (ISBN 978-953-51-3242-4). ‘Amino acids modifications to improve and fine-tune peptide-based hydrogels’. DOI: 10.5772/intechopen.68705


Canabady-Rochelle. Presentation of the MELISSA project. ANR Kick-off meeting, 11 December 2020.

Csire, G. Averlant-Petit, M.-C., Stefan, L., & Selmeczi, K. (2020, October). Iron(III)-chelating model peptides as bioinspired antioxidants. Oral session presentation at the Conférence FrenchBIC - Réunion annuelle du Groupe Français de Chimie Bio-Inorganique, online conference (France).

Csire G., Paris C., Canabady-Rochelle L., Stefan L., Selmeczi K. Iron(III)-che-lating model peptides as bioinspired antioxidants. LUE International Confe-rence “Biomolecules: Research & Development, Markets and Acceptability”. 23th-25th October 2019, Palma de Majorca, Spain. Flash presentation flash.

El Hajj, S., Canabady-Rochelle, L., Irankunda, R., Fries, I. & Gaucher, C. Investigating the antioxidant activity of metal-chelating peptides in soy hydrolysates on a cell-based ferroptosis model. 29th international conference for Functional Food Center (FFC). 29 – 30 Avril 2021, Dallas, TX, État-Unis. Oral presentation. Online conference.

El Hajj, S., Cakir-Kiefer, C., Stefan, L., Pontvianne, S., Arnoux, P., Paris, C., Muhr, L., Selmeczi, K., Desobry, S., Boschi-Muller, S., Gaucher, C., Cana-bady-Rochelle, L. Metal-chelating peptides in hydrolysates: biocatalysis production, characterization and screening. Seminar IMPACT BIOMOLECULE 2019. 23-25 Octobre, Palma de Mallorca, Espagne. Flash presentation.

34

El Hajj, S., Cakir-Kiefer, C., Arnoux, P., Stefan, L., Selmeczi, K., Muhr, L., Pasc, A., Boschi-Muller, S., Gaucher, C., & Canabady-Rochelle L. (2019, October). SPR screening of metal-chelating peptides for their antioxidant properties in laboratory-produced hydrolysates: effect of proteolysis conditions. Oral session presentation at the Conférence SFGP2019, Nantes (France).

Gaucher C. et Canabady-Rochelle L. Criblage de peptides chélateurs de métaux au sein d’hydrolysats pour leurs propriétés antioxydantes. Journée de restitution des PEPS Mirabelle, 12 Juillet 2018. Présentation orale.

Paris, C., Selmeczi K., Chaimbault P., Desobry S. & Canabady-Rochelle L. (2019, October). Criblage IP-RPLC-MS de peptides chélateurs de fer par analyse différentielle de spectre de masse. Oral session presentation at the Conférence SFGP2019, Nantes (France).

Paris C., Selmeczi K., Chaimbault P., Desobry S., Canabady-Rochelle, L. Optimization of a LCMS Method for screening of iron-chelating peptides by differential mass spectrometry analysis. Pittcon Conference, Chicago, Mc Cormick place, 1-5 Marsh 2020. Communication orale.

Canilho, N., Pasc, A., Cakir-Kiefer, C., Muhr, L., El Hajj, S., Paris, C., Selmeczi, K., Gaucher, C., Désobry, S., Schneider, R., Boschi, S., & Canabady-Rochelle, L. (2018, Paris). Oral session presentation at the Biocatalysis day organised by IAR, Paris (France).

Canabady-Rochelle, L. (2018, August). Metal chelating peptides for Skin and hair applications. Oral presentation at the BASF meeting, Nancy (France).

Canabady-Rochelle, L. (2018, May). Screening and separation of metal-che-lating peptides for nutrition and health applications. Oral presentation at the Impact Biomolecules, Scientific Seminary, Nancy (France).

Posters

Canabady-Rochelle, L., Selmeczi, K., Collin, S., El Hajj, S., Gaucher, C., Pasc, A., Muhr, L. & Boschi-Muller, S. Surface plasmon resonance: an original method for screening metal-chelating peptides in hydrolysates for their antioxidant properties. 21ème édition du congrès de la GFPP (Groupe Français des Peptides et des Protéines). 12-15 Mai, 2019, Amboise, France.

Csire G., Paris C., Canabady-Rochelle L., Stefan L., Selmeczi K. Iron(III)-che-lating model peptides as bioinspired antioxidants. LUE International Confe-rence “Biomolecules: Research & Development, Markets and Acceptability”. 23th-25th October 2019, Palma de Majorca, Spain. Poster

Canabady-Rochelle, L., Selmeczi, K., Collin, S., Pasc, A., Muhr, L., & Boschi-Muller, S. (2018, May). Surface Plasmon Resonance: an original method for screening of metal chelating peptides in hydrolysate for their antioxidant properties. Poster session presentation at the 21st GFPP meeting, Amboise (France).

Csire G., Paris C., Canabady-Rochelle L., Stefan L., & Selmeczi K. (2019, October). Iron(III)-chelating model peptides as bioinspired antioxidants. Conference LUE Biomolecules, Palma de Majorca (Spain).

Boschi-Muller, S., Paris, C., Désobry, S., Selmeczi, K., Chaimbault, P., Pasc, A., Cakir-Kiefer, C., Arrault, A., Stefan, A., Averlant-Petit, M.-C., Clarot, I., Leroy, P., Gaucher, C., Alem-Marchand, H., Celzard, A., Belaissaoui, B., Roizard, D., Schneider, R., Muhr, L., & Canabady-Rochelle, L. (2018, May). Screening and Separation of Metal Chelating Peptides for Nutrition and Health Applications. Poster session presentation at the Impact Biomole-cules, Scientific Seminary, Nancy (France).

El Hajj S., Cakir-Kiefer C., Stefan L., Pontvianne S., Arnoux P., Paris C., Muhr L., Selmeczi K., Desobry S., Boschi-Muller S., Gaucher C., & Canabady-Rochelle L. (2019, October). Metal-chelating peptides in hydrolysates: biocatalysis production, characterization and screening. Poster session presentation at the Conference LUE Biomolecules, Palma de Majorca (Spain).

El Hajj, S., Cakir-Kiefer, C., Stefan, L., Pontvianne, S., Arnoux, P., Paris, C., Muhr, L., Selmeczi, K., Desobry, S., Boschi-Muller, S., Gaucher, C., & L. Canabady-Rochelle. (2019, Mars). Metal-chelating peptides in hydrolysates: a way to valorize protein-containing co-products in a bioeconomy strategy. Séminaire de l’école doctorale SIMPPE, Nancy (France).

Gaucher, C., Clarot, I., Paris, C, Désobry, S., Cakir-Kiefer, C., Selmeczi, K., Arrault, A., Stefan, L. Averlant-Petit, M-C., Boschi-Muller, S., Muhr, L., & Canabady-Rochelle, L. (2018, May). Innovative screening methodology for metal chelating peptides with antioxidant properties using surface plas-mon resonance. Poster session presentation at the Impact Biomolecules, Scientific Seminary, Nancy (France).

Saidi, L., Stefan, L., Loulou, N., Canabady-Rochelle, L., Averlant-Petit, M. -C., & Selmeczi, K. (2018, May). Elucidation of mechanisms of metal ion-peptide interactions: Understand to innovate. Poster session presentation at the Impact Biomolecules, Scientific Seminary, Nancy (France).

Reti, B., Djehel T., Cakir-Kiefer C., Belassaoui B., Roizard D., Pasc A., Ca-nabady-Rochelle L. et Celzard A. Antioxidant peptide production through carbon reactive separators. Atelier Scientific ICEEL, 25 mai 2018. Poster.

Tan, J., Ren, J., Carisey, B. Martins-Libone, H., Pontvianne, S., Selmeczi, K., Paris, C., Boschi-Muller, S., Muhr, L., & Canabady-Rochelle L. (2019, July). Simulation de la séparation de peptides chélateurs de métaux par chromatographie IMAC à partir de constantes d’affinité déterminées par RPS. Poster session presentation at the Journée technique “Procédés de separation”, Lyon (France).

Dancs, Á., Gajda, T., & Selmeczi K. (2018, May). The role of deprotonated amide nitrogens in peptide: an acid/base site for facilitating substrate binding in catechol oxidation. Poster session presentation at the Copper Bioinorganic Chemistry Symposium (CuBICS 2018), Marseille (France).

El Hatmi, H., Jrad, Z., Loussaief, O., Nasri, W. Khorchani, T., & Cana-bady-Rochelle, L. (2017, July). Fermentation of dromedary (Camelus dro-medarius) milk by Lactococcus lactis subsp. cremoris and Streptococcus macedonicus as a potential alternative of cow milk. Poster session pre-sentation at the 16ième Conférence de la Société de Génie des Procédés, Nancy (France).

Paris, C., Selmeczi, K., Boschi-Muller, S., Pontvianne, S., Muhr, L., Chaimbault, P., & Laetitia Canabady-Rochelle, L. (2017, July). Criblage rapide par IMAC-MS de peptides chélateurs de métaux pour leurs propriétés antioxydantes. Poster session presentation at the 16ème Conférence de la Société de Génie des Procédés, Nancy (France).

Paris C., Selmeczi K., Chaimbault P., Desobry S. and Canabady-Rochelle L. IP-RPLC-MS Screening of iron(II) chelating peptides by differential analysis of mass spectra. LUE International Conference “Biomolecules: Research & Development, Markets and Acceptability”. 23th-25th October 2019, Palma de Majorca, Spain. Poster.

Canabady-Rochelle, L., Selmeczi, K., Collin, S., Pacs, A., Muhr L., & Boschi-Mul-ler, S. (2017, June). SPR Screening of Metal chelating Peptides for their Antioxidant Properties. Poster session presentation at the 14th International Conference of Applied Bioorganic Chemistry, Toulouse (France).

34

El Hajj, S., Cakir-Kiefer, C., Arnoux, P., Stefan, L., Selmeczi, K., Muhr, L., Pasc, A., Boschi-Muller, S., Gaucher, C., & Canabady-Rochelle L. (2019, October). SPR screening of metal-chelating peptides for their antioxidant properties in laboratory-produced hydrolysates: effect of proteolysis conditions. Oral session presentation at the Conférence SFGP2019, Nantes (France).

Gaucher C. et Canabady-Rochelle L. Criblage de peptides chélateurs de métaux au sein d’hydrolysats pour leurs propriétés antioxydantes. Journée de restitution des PEPS Mirabelle, 12 Juillet 2018. Présentation orale.

Paris, C., Selmeczi K., Chaimbault P., Desobry S. & Canabady-Rochelle L. (2019, October). Criblage IP-RPLC-MS de peptides chélateurs de fer par analyse différentielle de spectre de masse. Oral session presentation at the Conférence SFGP2019, Nantes (France).

Paris C., Selmeczi K., Chaimbault P., Desobry S., Canabady-Rochelle, L. Optimization of a LCMS Method for screening of iron-chelating peptides by differential mass spectrometry analysis. Pittcon Conference, Chicago, Mc Cormick place, 1-5 Marsh 2020. Communication orale.

Canilho, N., Pasc, A., Cakir-Kiefer, C., Muhr, L., El Hajj, S., Paris, C., Selmeczi, K., Gaucher, C., Désobry, S., Schneider, R., Boschi, S., & Canabady-Rochelle, L. (2018, Paris). Oral session presentation at the Biocatalysis day organised by IAR, Paris (France).

Canabady-Rochelle, L. (2018, August). Metal chelating peptides for Skin and hair applications. Oral presentation at the BASF meeting, Nancy (France).

Canabady-Rochelle, L. (2018, May). Screening and separation of metal-che-lating peptides for nutrition and health applications. Oral presentation at the Impact Biomolecules, Scientific Seminary, Nancy (France).

Posters

Canabady-Rochelle, L., Selmeczi, K., Collin, S., El Hajj, S., Gaucher, C., Pasc, A., Muhr, L. & Boschi-Muller, S. Surface plasmon resonance: an original method for screening metal-chelating peptides in hydrolysates for their antioxidant properties. 21ème édition du congrès de la GFPP (Groupe Français des Peptides et des Protéines). 12-15 Mai, 2019, Amboise, France.

Csire G., Paris C., Canabady-Rochelle L., Stefan L., Selmeczi K. Iron(III)-che-lating model peptides as bioinspired antioxidants. LUE International Confe-rence “Biomolecules: Research & Development, Markets and Acceptability”. 23th-25th October 2019, Palma de Majorca, Spain. Poster

Canabady-Rochelle, L., Selmeczi, K., Collin, S., Pasc, A., Muhr, L., & Boschi-Muller, S. (2018, May). Surface Plasmon Resonance: an original method for screening of metal chelating peptides in hydrolysate for their antioxidant properties. Poster session presentation at the 21st GFPP meeting, Amboise (France).

Csire G., Paris C., Canabady-Rochelle L., Stefan L., & Selmeczi K. (2019, October). Iron(III)-chelating model peptides as bioinspired antioxidants. Conference LUE Biomolecules, Palma de Majorca (Spain).

Boschi-Muller, S., Paris, C., Désobry, S., Selmeczi, K., Chaimbault, P., Pasc, A., Cakir-Kiefer, C., Arrault, A., Stefan, A., Averlant-Petit, M.-C., Clarot, I., Leroy, P., Gaucher, C., Alem-Marchand, H., Celzard, A., Belaissaoui, B., Roizard, D., Schneider, R., Muhr, L., & Canabady-Rochelle, L. (2018, May). Screening and Separation of Metal Chelating Peptides for Nutrition and Health Applications. Poster session presentation at the Impact Biomole-cules, Scientific Seminary, Nancy (France).

El Hajj S., Cakir-Kiefer C., Stefan L., Pontvianne S., Arnoux P., Paris C., Muhr L., Selmeczi K., Desobry S., Boschi-Muller S., Gaucher C., & Canabady-Rochelle L. (2019, October). Metal-chelating peptides in hydrolysates: biocatalysis production, characterization and screening. Poster session presentation at the Conference LUE Biomolecules, Palma de Majorca (Spain).

El Hajj, S., Cakir-Kiefer, C., Stefan, L., Pontvianne, S., Arnoux, P., Paris, C., Muhr, L., Selmeczi, K., Desobry, S., Boschi-Muller, S., Gaucher, C., & L. Canabady-Rochelle. (2019, Mars). Metal-chelating peptides in hydrolysates: a way to valorize protein-containing co-products in a bioeconomy strategy. Séminaire de l’école doctorale SIMPPE, Nancy (France).

Gaucher, C., Clarot, I., Paris, C, Désobry, S., Cakir-Kiefer, C., Selmeczi, K., Arrault, A., Stefan, L. Averlant-Petit, M-C., Boschi-Muller, S., Muhr, L., & Canabady-Rochelle, L. (2018, May). Innovative screening methodology for metal chelating peptides with antioxidant properties using surface plas-mon resonance. Poster session presentation at the Impact Biomolecules, Scientific Seminary, Nancy (France).

Saidi, L., Stefan, L., Loulou, N., Canabady-Rochelle, L., Averlant-Petit, M. -C., & Selmeczi, K. (2018, May). Elucidation of mechanisms of metal ion-peptide interactions: Understand to innovate. Poster session presentation at the Impact Biomolecules, Scientific Seminary, Nancy (France).

Reti, B., Djehel T., Cakir-Kiefer C., Belassaoui B., Roizard D., Pasc A., Ca-nabady-Rochelle L. et Celzard A. Antioxidant peptide production through carbon reactive separators. Atelier Scientific ICEEL, 25 mai 2018. Poster.

Tan, J., Ren, J., Carisey, B. Martins-Libone, H., Pontvianne, S., Selmeczi, K., Paris, C., Boschi-Muller, S., Muhr, L., & Canabady-Rochelle L. (2019, July). Simulation de la séparation de peptides chélateurs de métaux par chromatographie IMAC à partir de constantes d’affinité déterminées par RPS. Poster session presentation at the Journée technique “Procédés de separation”, Lyon (France).

Dancs, Á., Gajda, T., & Selmeczi K. (2018, May). The role of deprotonated amide nitrogens in peptide: an acid/base site for facilitating substrate binding in catechol oxidation. Poster session presentation at the Copper Bioinorganic Chemistry Symposium (CuBICS 2018), Marseille (France).

El Hatmi, H., Jrad, Z., Loussaief, O., Nasri, W. Khorchani, T., & Cana-bady-Rochelle, L. (2017, July). Fermentation of dromedary (Camelus dro-medarius) milk by Lactococcus lactis subsp. cremoris and Streptococcus macedonicus as a potential alternative of cow milk. Poster session pre-sentation at the 16ième Conférence de la Société de Génie des Procédés, Nancy (France).

Paris, C., Selmeczi, K., Boschi-Muller, S., Pontvianne, S., Muhr, L., Chaimbault, P., & Laetitia Canabady-Rochelle, L. (2017, July). Criblage rapide par IMAC-MS de peptides chélateurs de métaux pour leurs propriétés antioxydantes. Poster session presentation at the 16ème Conférence de la Société de Génie des Procédés, Nancy (France).

Paris C., Selmeczi K., Chaimbault P., Desobry S. and Canabady-Rochelle L. IP-RPLC-MS Screening of iron(II) chelating peptides by differential analysis of mass spectra. LUE International Conference “Biomolecules: Research & Development, Markets and Acceptability”. 23th-25th October 2019, Palma de Majorca, Spain. Poster.

Canabady-Rochelle, L., Selmeczi, K., Collin, S., Pacs, A., Muhr L., & Boschi-Mul-ler, S. (2017, June). SPR Screening of Metal chelating Peptides for their Antioxidant Properties. Poster session presentation at the 14th International Conference of Applied Bioorganic Chemistry, Toulouse (France).

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Canabady-Rochelle, L., Paris, C., Selmeczi, K., Gaucher, C., Clarot, I., Leroy, P., Alem, H., Schneider, R., Chaimbault, P., Muhr, L., & Boschi-Muller, S. (2016, Paris). Screening and purification of metal chelating peptides in hydrolysates for their antioxidant properties. Poster session presentation at the 17th International Conference on Oxidative Stress Reduction, Redox Homeostasis and Antioxidants, Institut Pasteur - Paris (France).

Other communications

El Hajj, S., Csire, G., Paris, C., Irankunda, R., Girardet, J.-M, Alem H., Desobry, S., Schneider, R., Boschi-Muller, S., Stefan, L., Selmeczi, K., Muhr, L, Gaucher, C. and Canabady-Rochelle, L. Metal-Binding Peptides: Production, Screening & Separation for various applications. 16 Novembre, 2020, Nancy, France. Cours-conférence (3h) aux étudiant.e.s de M2 de l’ENSAIA (UL).

Organisation of scientific events

International Conference on Metal-Binding Peptides: Methodologies and Applications, 7th-10th of July 2020, ENSIC, Nancy. Chair(wo)men : Laetitia Canabady-Rochelle et Peter Faller (Université de Strasbourg). Post-poned to 5th to the 8th July 2022.

Scientific mediation

Gaucher C. (2018, February). Impact Biomolecules. Oral presentation at the 2nd meeting of the NutRedOx COST Action CA16112, Palma de ma-jorque (Spain)

Schneider R., Faller P. & Canabady-Rochelle L. Article sur la plateforme The Conversation France. 9 Janvier 2020. « Quand des « mini-protéines » mettent en cage des métaux. https://theconversation.com/quand-des-mini-proteines-mettent-en-cage-des-metaux-127861.

Interview sur ‘’Factuel l’info de l’université de Lorraine’’ Portrait de chercheuse, Journée Internationale des droits de la femme, 8 mars 2020. http://factuel.univ-lorraine.fr/node/13915

35


Canabady-Rochelle, L., Paris, C., Selmeczi, K., Gaucher, C., Clarot, I., Leroy, P., Alem, H., Schneider, R., Chaimbault, P., Muhr, L., & Boschi-Muller, S. (2016, Paris). Screening and purification of metal chelating peptides in hydrolysates for their antioxidant properties. Poster session presentation at the 17th International Conference on Oxidative Stress Reduction, Redox Homeostasis and Antioxidants, Institut Pasteur - Paris (France).

Other communications

El Hajj, S., Csire, G., Paris, C., Irankunda, R., Girardet, J.-M, Alem H., Desobry, S., Schneider, R., Boschi-Muller, S., Stefan, L., Selmeczi, K., Muhr, L, Gaucher, C. and Canabady-Rochelle, L. Metal-Binding Peptides: Production, Screening & Separation for various applications. 16 Novembre, 2020, Nancy, France. Cours-conférence (3h) aux étudiant.e.s de M2 de l’ENSAIA (UL).

Organisation of scientific events

International Conference on Metal-Binding Peptides: Methodologies and Applications, 7th-10th of July 2020, ENSIC, Nancy. Chair(wo)men : Laetitia Canabady-Rochelle et Peter Faller (Université de Strasbourg). Post-poned to 5th to the 8th July 2022.

Scientific mediation

Gaucher C. (2018, February). Impact Biomolecules. Oral presentation at the 2nd meeting of the NutRedOx COST Action CA16112, Palma de ma-jorque (Spain)

Schneider R., Faller P. & Canabady-Rochelle L. Article sur la plateforme The Conversation France. 9 Janvier 2020. « Quand des « mini-protéines » mettent en cage des métaux. https://theconversation.com/quand-des-mini-proteines-mettent-en-cage-des-metaux-127861.

Interview sur ‘’Factuel l’info de l’université de Lorraine’’ Portrait de chercheuse, Journée Internationale des droits de la femme, 8 mars 2020. http://factuel.univ-lorraine.fr/node/13915

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Documents

FINAL REPORT LUE IMPACT Biomolecules project