Filling a RackMatrix with SolventNeeds version 1.1.3.8A

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Filling a Rack Matrix with Solventin Two Steps - Fast

Introduction

This method is useful, if you want to:

• Dispense solvent in a rack.• Dissolve solid samples that are supplied in a rack.• Dilute samples that are supplied in a rack.

This document describes

How a complete rack or several vials in a rack can be filled with a user defined amount of solvent (Solvent from dilutor or small amounts from tray).

Two procedures will be used:

• The first fills the syringe with the total solvent amount. • The second quickly dispenses equal solvent parts into the selected

destinations.

Tip:

Prep-Gilson always requires source and destination positions. In this case only the second procedure needs the selection of destination positions.

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Filling a Rack Matrix with Solventin Two Steps - Fast

2. ”Manual destination selection“ is checked.

3. ”Clean ... “ is unchecked.

Step 1: Check Settings

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Filling a Rack Matrix with Solventin Two Steps - Fast

Open procedure editor dialog

Two main procedures will be defined:

1. Fill syringe with the total solvent amount 1200µl for 12 tubes.Name: ”Load Syringe DMSO 1200.gsp“

2. Dispense partial solvent amount 100µl into one destination.Name: ”Dispense Volume 100.gsp “(with subprocedure ”Sub Disp Volume 100.gsp “)

Step 1: Check Settings

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Filling a Rack Matrix with Solventin Two Steps - Fast

2. Select the source type ”Solvent in Tray“.

3. Select your own values for:- Solvent name- Volume (Speed ...)

Tip: Never forget ”Update“ and ”Save“ after any modifications.

1. Check the file name ”Dispense Volume 100.gsp“

Step 2: Edit Procedure ”Load Syringe DMSO 1200.gsp“

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Filling a Rack Matrix with Solventin Two Steps - Fast

Press the ”Edit“ button to edit or create a procedure of type ”Dispense“.

Step 3a: Edit Dispense Subprocedure ”Sub Disp Volume 100.gsp“

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Filling a Rack Matrix with Solventin Two Steps - Fast

Select your own values for:- Volume - Z offset height (Speed ...).

Check the file name ”Sub Disp Volume 100.gsp“.

Step 3a: Edit Dispense Subprocedure ”Sub Disp Volume 100.gsp“

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Filling a Rack Matrix with Solventin Two Steps - Fast

Select the source type”Gas Gap“.

Select your dispense procedure.

Check the file name ”Dispense Volume 100.gsp“.

Volume = 0

Step 3b: Edit Dispense Main Procedure ”Dispense Volume 100.gsp“

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Filling a Rack Matrix with Solventin Two Steps - Fast

Start Automation.

1. Select procedure ”Load Syringe DMSO 1200.gsp“.

2. Select one dummy source and destination position (ones not used in the procedure!).

Tip:Solvent not used in procedure!

3. Create Order and do not start with ”OK“.

Step 4: Start Automation to Fill Sample Loop

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Filling a Rack Matrix with Solventin Two Steps - Fast

1. Select procedure “Dispense Volume 100.gsp“.

2. Select 12 source and destination positions (destinations will be used).

Tip: Solvent not used in procedure!

3. Create orders and close dialog with “OK“.

Step 5: Create Orders to Fill Rack Matrix

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Filling a Rack Matrix with Solventin Two Steps - Fast

Step 6: Running Preparation

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Filling a Rack Matrix with Solventin Two Steps - Fast