FIGURE Microautoradiography. Steps for preparing a microautoradiograph from a cell culture. FIGURE Microautoradiographs. Examples of [ 3 H]thymidine incorporation into a cell onolayer. Normal glial cells were incubated with 0.1 Ci/mL (3.7 KBq/mL), 200 Ci/mmol (7.4 GBq/mol), of [ 3 H]thymidine for 24 h, washed, and processed as described in Protocol (a) Typical densely labeled nuclei, suitable for determining the labeling index (see Protocol 20.11). (b) A similar culture, infected with mycoplasma showing [ 3 H]thymidine incorporation in the cytoplasm. (See also Plates 13c, d, 14d). FIGURE Somatic Cell Hybridization. Selection of hybrid cells after fusion (see Section 27.7). FIGURE Production of Hybridomas. Schematic diagram of the production of hybridoma clones capable of secreting monoclonal antibodies.