Upload
john-ball
View
236
Download
1
Tags:
Embed Size (px)
Citation preview
Figure 1
Renilla LucTkpRL-Tk
Firefly LucLexApL6NLuc NRE
pLexA-p65 p65 LexA MPSV
pLexA-p65
pL6NLuc
NRF expression vectors - - NRF(1-380) NRF(1-690)
- + + ++ + + +
Figure 2
p65 RHD
p65
endogenous p65
B
NR
F(1-
380)
TAP
+ p6
5
NR
F(1-
380)
TAP
+ p6
5RH
D
NRF(1-380)TAP
NR
F(1-
380)
TAP
+ p6
5
NR
F(1-
380)
TAP
+ p6
5RH
D
total bound
p65 bound
p50 bound
A
Control
NRF-Strep
1
26
51
76
126
51
76
Spots
Spots
B
spot peptide sequence
1 1MDELFPLIFPAEPAQ15
2 4LFPLIFPAEPAQASG18
3 7LIFPAEPAQASGPYV21
4 10PAEPAQASGPYVEII24
. .
126
51
76
RRL
A
Figure 3
85 114 199
246 274 309
A
spot peptide sequence
1 88HELVGKDC95
2 89ELVGKDCR96
3 90LVGKDCRD97
21 87PHELVGKDC95
22 88HELVGKDCR96
23 89ELVGKDCRD97
42 86HPHELVGKDC95 43 87PHELVGKDCR96
44 88HELVGKDCRD97
.
.
peptide length
8 aa8 aa8 aa
9 aa9 aa9 aa
10 aa10 aa10 aa
.
.
.
.
Figure 4
NRF-Strep
265176
1
101126151176
spots peptide length
8-9 aa8
9
10
11
12
13
14
15
9-10 aa10-11 aa11-12 aa12-13 aa13-14 aa14-15 aa15 aa
B
81 PPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQ 114
Figure 5
1
26
SpotsNRF-Strep
B
spot peptide sequence
1 199VNRNSGSCLGGDEIF213
2 200NRNSGSCLGGDEIFL214
3 201RNSGSCLGGDEIFLL215
. .
199 VNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHR 246
A
Figure 6
A 274 RPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTY 306
spot peptide sequence
1 274RPSDRELSEPMEFQY288
2 274APSDRELSEPMEFQY288
3 274RASDRELSEPMEFQY288
4 274RPADRELSEPMEFQY288
18 277DRELSEPMEFQYLPD291
19 277ARELSEPMEFQYLPD291
20 277DAELSEPMEFQYLPD291
21 277DRALSEPMEFQYLPD291
. .
. .
B
NRF-Strepspots
265176
1
101