7
Figure 1 C. LDH release (LDH assay, O.D.) D. Cell viability *** (MTT test, O.D.) E. Cell proliferation (BrdU incorporation, O.D.) B. Cell death area (mm 2 ) A. Wound closure Hyperoxia (mm 2 per 24 h) *** 0 0.1 0.2 0.3 0 0.1 0.2 0.3 0.4 0.5 *** 0 0.25 0.5 0.75 1 *** 0 0.25 0.5 0.75 1 1.25 0 0,05 0,1 0,15 Control in room air

Figure 1

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A. Wound closure. B. Cell death area. C. LDH release. (mm 2 per 24 h). (mm 2 ). (LDH assay, O.D.). 1. 0.5. 0.3. ***. ***. ***. 0.4. 0.75. 0.2. 0.3. 0.5. 0.2. 0.1. 0.25. 0.1. 0. 0. 0. D. Cell viability. E. Cell proliferation. (MTT test, O.D.). - PowerPoint PPT Presentation

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Page 1: Figure 1

Figure 1

C. LDH release(LDH assay, O.D.)

D. Cell viability

***

(MTT test, O.D.)

E. Cell proliferation(BrdU incorporation, O.D.)

B. Cell death area(mm2)

A. Wound closure

Hyperoxia

(mm2 per 24 h)

***

0

0.1

0.2

0.3

0

0.1

0.2

0.3

0.4

0.5

***

0

0.25

0.5

0.75

1

***

0

0.25

0.5

0.75

1

1.25

0

0,05

0,1

0,15

Control inroom air

Page 2: Figure 1

Figure 2

C. LDH releaseB. Cell death areaA. Wound closure

D. Cell viability E. Cell proliferation

*

0

20

40

60

80

100

120

140

160

180 +

0

20

40

60

80

100

120

0

25

50

75

100

125

***

+ Control in room air

Hyperoxia + KGF 10ng/ml

Hyperoxia + KGF 100ng/ml

Hyperoxia

0

50

100

150

200

250

300

350*** ****** +

***

0

100

200

300

400

500

600

700

800

900

1000

1100***

+++*

Page 3: Figure 1

Survival rate

Time (days)

0

0.2

0.4

0.6

0.8

1

0 2 4 6 8 10

Figure 3

Page 4: Figure 1

Figure 4

0

2.5

5

7.5

10

12.5

15

17.5

20

22.5

Cells in BAL (x 10-4 / ml)

Control KGF Control KGF

Room air Hyperoxia

**

Page 5: Figure 1

Figure 5

Room air0

1

2

3

4

5

6

7

8

DNA content (µg / mg ww)

Hyperoxia

******

Page 6: Figure 1

Figure 6

DC

BA

100 µm

Page 7: Figure 1

Figure 7

***

§

room air hyperoxia room air hyperoxia

day 5 day 10

Caspase-3 protein

0

20

40

60

80

100

120% of day 5 room-air control

***

35kDa

19kDa17kDa

Ponceau S


Figure 1 shows the apparatus. Figure 1

Figure 1 shows the apparatus. Figure 1

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