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Field of View 4X
Field of View 10X
Field of View 40X
4X
10X
40X
Depth of Field – the vertical distance of an object that is in focus. When magnification is increased, less of the object is in focus (depth of field decreases) – BUT greater detail of the area in focus can be seen.
Depth of Field
Not in focus
In focus
Stage Micrometer
Ocular Micrometer
Stage Micrometer = 10 units =10*0.01mm = .1mm
Ocular Micrometer = 9 units
1 ocular division = .1mm / 9 units = 0.011mm
Each small division = 0.011 mm
This cell spans across 11 ocular divisions. How wide is this cell? 11*0.011=0.121mm.
CytoplasmNucleus
Plasma membrane
Generic Animal Cell
Eukaryotic – With a membrane bound nucleus
Animal cell anatomy
Plant cell anatomy
Flagellum
Cell WallCytoplasm
Plasma Membrane
Ribosome
Nucleoid Region
Generic Prokaryote Cell
Prokaryotic – Without a membrane bound nucleus