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Fernando
Liza Santuza Omar
Alan Júlio
Paola
Lucas
ÍtaloRaphael
Carlos
Marianna
PriscilaClara
Our team
Minas Gerais, Brazil
Where we came from
Where we came from
Belo Horizonte - MG
Where we came from
Universidade Federal de Minas Gerais (UFMG)
Acute Coronary Syndrome (ACS)
NIH Medline PlusWinter 2009 Issue: Volume 4 Number 1
A worldwide problem
Acute Coronary Syndrome (ACS)A worldwide problem
WHO, 2013
Acute Coronary Syndrome (ACS)A Brazilian problem
Teich et al., Rev Bras Cardiol., 2011
Acute Coronary Syndrome (ACS)A challenge
• To develop a sensitive device capable ofdetecting and integrating the signals of ACSbiomarkers, setting a prognosis.
Aiming high
Biomarkers
Tang et al., N Engl J Med., 2013
Trimethylamine-N-OxideTMAO: Introduction
‘
Trimethylamine-N-OxideTMAO: Construction
TorS
TorT
TMAO
TorT
TorR
TMAO
TorR*
pTorCAD RFP
BBa_K1086000-------- Ba_K1086002 -------
‘
Trimethylamine-N-OxideTMAO: Construction
TorS
TorT
TMAO
TorT
TorR
TMAO
TorR*
pTorCAD RFP
TorT + TMAO
2 TorT + 2 TMAO + TorS
Trimethylamine-N-OxideTMAO: Modeling
Trimethylamine-N-OxideTMAO: Results
TorCAD+RFP ----- TMAO
Fluorescence/Absorbance
Ischemia Modified AlbuminIMA: Introduction
Normal patient blood
(Cobalt added)
Ischemic patient blood
(Cobalt added)
AlbuminIMA
Ischemia Modified AlbuminIMA: Construction
pRCNA YFP
-------- Ba_K1086001 --------
Normal patient blood
(Cobalt added)
Ischemic patient blood
(Cobalt added)
AlbuminIMA
Ischemia Modified AlbuminIMA: Modeling
Ischemia Modified AlbuminIMA: Modeling
Albumin 90 µM
Ischemia Modified AlbuminIMA: Modeling
Cobalt 25 µM
Ischemia Modified AlbuminIMA: Results
RCNA+YFP ----- Cobalt
Free Cobalt Assay:
Fluorescence/Absorbance
Ischemia Modified AlbuminIMA: Results
Bovine Serum Albumin Assay:
Fluorescence
RCNA+YFP ----- Cobalt
Ischemia Modified AlbuminIMA: Results
RCNA+YFP ----- IMA+Cobalt
Ischemic and Non-Ischemic Mice Sera Assay:
Fluorescence
Non-Ischemic
Ischemic
Talking with specialistsHuman Practices
Doctor Antonio Luiz Pinho Ribeiro
ETHICS BIOSAFETY
Neuza Antunes Rodrigues
Interest over time. Web Search. Worldwide, 2004 - present.
Scientific disseminationHuman Practices
Interest over time. Web Search. Worldwide, 2004 – present
English x Portuguese
GoalsHuman Practices
Cardbio
Impact
Thinking
outside
the box
Talking
with
specialists
Collaboration
Scientific
dissemination
Scientific disseminationHuman Practices
Our team
Scientific disseminationHuman Practices
Our team
Our university
Scientific disseminationHuman Practices
Scientific disseminationHuman Practices
Our team
Our university
Public schools
Scientific disseminationHuman Practices
UFMG & Escolas
Scientific disseminationHuman Practices
Missions
1. Fuel from sunlight
2. Microplastic hazard on the oceans
3. Spoiled meat
4. Malaria and artemisin
5. Celiac disease
6. Space exploration
7. Our own project
The Brickard Game
Scientific disseminationHuman Practices
Our team
Our university
Public schools
Our state
Scientific disseminationHuman Practices
Scientific disseminationHuman Practices
Our team
Our university
Public schools
Our state
Brazil
Scientific disseminationHuman Practices
Website
Scientific disseminationHuman Practices
Wikipedia
facebook.com/igemufmg
twitter.com/igemufmg
Scientific disseminationHuman Practices
Video aboutSynthetic Biology
Newspaper
GoalsHuman Practices
Cardbio
Impact
Thinking
outside
the box
Talking
with
specialists
Collaboration
Scientific
dissemination
Thinking outside the boxHuman Practices
Hackspace
Thinking outside the boxHuman Practices
Our song!
GoalsHuman Practices
Cardbio
Impact
Thinking
outside
the box
Talking
with
specialists
Collaboration
Scientific
dissemination
CollaborationHuman Practices
CollaborationHuman Practices
Jamboré – Meeting of Brazilian teams
Achievements
Assembly, registry and characterization of new biobricks:
RCNA + YFP (BBa_K1086001)
TorCAD (BBa_K1086000)
TorCAD+ RFP (BBa_K1086002)
Modeling of our systems.
Expand the knowledge about synthetic biology in our University,
our State and our Country.
Thanks
Sponsors
Other supporters
Laboratório de Genética Bioquímica Laboratório de Sinalização Celular e NanobiotecnologiaLaboratório de Genômica e Genética de Parasitos Laboratório de Imunoquímica de Toxinas NaturaisLaboratório de Sistema de InformaçãoLaboratório de Angiogênese
Professor Gastone CastellaniProfessora Andréa Mara MacedoNeuza Antunes RodriguesJuliana PimentaRosalvo Fogaça –UFPRIzabella Pena Neshich – UNICAMPDoutor Antonio Luiz Pinho RibeiroAndré Luis Martins Reis – Johns Hopkins
Thanks
Crowd funding donators
Denis RogerPedro Henrique Nascimento de AguiarFilipe Silva VillelaSabrina LimaMarina DinizDiego Pessoa SantosGraciela Kunrath LimaBel BitarIsabela F. PratesMarcela Segatto do CarmoHenrique TeichmannNicole SchererWagner ArbexGlória FrancoNaíla Barbosa da Costa
Fernanda Santos FreitasPedro E. C. VieiraFrancislon Silva de OliveiraLucas Antunes de Siqueira CostaPedro Pinho TeixeiraJarbas RohrMainá BitarLeonardo Lucas Carnevalli DiasLuandy BaiãoWesley Júnio da SilvaMichele AraújoNeuza AntunesJuliano LealCeres Luciana AlvesMaíra FreireAmanda Campelo
Thank you!
