FEASIBILITY STUDY - Universitatea de Medicina si … ENG.doc · Web viewFEASIBILITY STUDY THE SECTORAL OPERATIVE PROGRAMME – THE DEVELOPEMENT OF ECONOMIC COMPETITIVITY PRIORITY

University of Medicine and Pharmacy CRAIOVA, ROMANIA intergroup engineering

FEASIBILITY STUDY

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THE SECTORAL OPERATIVE PROGRAMME – THE DEVELOPEMENT OF ECONOMIC COMPETITIVITY

PRIORITY AXE II: RDI INFRASTRUCTURE INVESTMENT

- TARGET - CENTRE FOR TREATMENT AND RESEARCH IN GASTROENTEROLOGY

BASED ON IMAGING METHODS AND MOLECULAR TECHNIQUES

Studiu de fezabilitate – TARGET SF. 2

CONTENT:

CONTENT: .....................................................................................................................................3Acronyms: .....................................................................................................................................51 GENERAL DATA .............................................................................................................61.a Name of investment objective .....................................................................................61.b The location (county, city, street, number) .........................................................................61.c The investment titular .............................................................................................................61.d The investment beneficiary .................................................................................................61.e The study elaborator .............................................................................................................62 GENERAL INFORMATION CONCERNING THE PROJECT .....................................62.a The actual situation and information about the responsible entity

of project implementation .......................................................................................................62.b Investment description ................................................................................................122.b.1 The conclusions of pre-feasibility study or of detailed investment plan

(if it was elaborated) regarding actual situation, the necessity and opportunity of investment promotion and technico-economic selected script as well ................................................12

2.b.2 Technical-economic scenarios ....................................................................................392.b.3 Constructive, functional and technological description, in any case ....................................422.c Technical data of investment ................................................................................................982.c.1 Area and location ............................................................................................................982.c.2 Judicial status of the ground .............................................................................................982.c.3 Situation of definite ground occupations ........................................................................982.c.4 Ground studies ............................................................................................................982.c.5 Main characteristics of constructions inside investments objective and the constructive

variants of investment’s achievement ...............................................................................982.c.6 Existent situation of utility and consumption analysis ..............................................1112.c.7 Conclusions of evaluation impact over environment .........................................................1112.d Achievement duration and principal stages, graphic of investment’s achievement ..........1123 ESTIMATED COST OF THE INVESTMENT ..................................................................1143.a Total value with detail on the structure of general list ..............................................1143.b The space out of corroborated costs with the achievement graphic of the investment .......1174 ANALYSIS OF COST-BENEFIT ......................................................................................1204.a Identification of the investment and defining of the objectives ..................................1204.b Analysis of options ..........................................................................................................1214.c Financial analysis ..........................................................................................................1224.d Economic analysis ..........................................................................................................1234.e Sensitivity analysis ..........................................................................................................1234.f Risk analysis ......................................................................................................................1235 FINANCE SOURCES OF THE INVESTMENT ...............................................................1256 ESTIMATIONS REGARDING LABOUR EMPLOYERS THROUGH THE

ACHIEVEMENT OF INVESTMENT ......................................................................1266.a Number of labor places created in the execution place ..............................................1266.b Number of places created in the operation place ..........................................................1267 THE PRINCIPAL TECHNICO-ECONOMIC INDEXES OF INVESTMENT ................1278 APROVALS AND AGREEMENTS .................................................................................1318.a Notice of investment beneficiary regarding the necessity

and the opportunity of investment ..................................................................................1318.b The city planning certificate ..........................................................................................131

Studiu de fezabilitate – TARGET SF 3

8.c The principle approvals regarding the utilities availability (thermal and electric energy, methane gas, communications, water-sewerage etc) .........................................................131

8.d The environmental approval ..............................................................................................1318.e Other specific approvals and principle agreements ..........................................................131

Appendix 1 A – 1P Appendix 2Appendix 3Appendix 4Appendix 5Appendix 6Appendix 7Appendix 8Appendix 9


ACRONYMS:

MP Master PlanFS Feasibility study R/D Research – DevelopmentSOP-ECD Sectoral Operation Program – Economic Competitively Development SOP-ECD/RDI Sectoral Operation Program – Economic Competitively Development/

Research – Development – Innovation SOP-HRD Sectoral Operation Program – Human Resources DevelopmentFP7 7th Framework Program PET Positron Emission TomographyCT Computer TomographyMRI Magnetic Resonance ImagingMRCP Magnetic Resonance Colangio-Pacreatography MRS Magnetic Resonance Spectroscopy Module ERCP Endoscopic CholangiopancreatographyAFI Autofluorescence EndoscopyLIFS Laser-Induced Fluorescence Spectroscopy CLE Confocal Laser Endomicroscopy (CLE)MCE Magnification ChromoendoscopyNBI Narrow Band ImagingEUS Endoscopic UltrasoundNOTES Natural Orifice Transluminal Endoscopic Surgery LS Laparoscopic Surgery ICC Immunocytochemistry IHC Immunohistochemistry ISH In Situ Hybridization LCM Laser Capture Microdissection CGH Comparative Genome Hybridization Technique MSI Microsatelitic Instability SNP Single Nucleotide Polymorphism LOH Loss of Heterozygosity State RT-PCR Real-time Polymerase Chain ReactionMALDI-TOF-MS Matrix Array Laser/Desorption Ionization Time of Flight Mass

Spectrometry AFM Atomic Force Microscopy LSCM Laser Scanner Confocal Microscopy AI Artificial Intelligence


1 GENERAL DATA

1.a Name of investment objective

TARGET – CENTRE FOR TREATMENT AND RESEARCH IN GASTROENTEROLOGY BASED ON IMAGING METHODS AND MOLECULAR TECHNIQUES

1.b The location (county, city, street, number)

LOCATION AThe old building of UMF Craiova, Dolj, Craiova, Petru Rares Street, No 2 (200349)

LOCATION BThe new building of UMF Craiova, Dolj, Craiova, 1 Mai Boulevard, No 66-68 (200638)

1.c The investment titular

UNIVERSITY OF MEDICINE AND PHARMACY CRAIOVA

1.d The investment beneficiary

UNIVERSITY OF MEDICINE AND PHARMACY CRAIOVA

1.e The study elaborator

S.C. INTERGROUP ENGINEERING S.R.L.

2 GENERAL INFORMATION CONCERNING THE PROJECT

2.a The actual situation and information about the responsible entity of project implementation

Situation of research and research infrastructure at national, international and institu-tional levelThe use of technology innovation in medicine has lead to a constant growth of expenses in the international healthcare system. This trend will continue to increase the mean expectancy of life, which will subsequently lead to the desire of new treatments and better medications. Parallel with this development, a new discipline appeared, the sanitary economy, orienting the resources for a more efficient use. A part of this concept is the early diagnosis which will allow an optimal allocation of human resources and materials by the achievement of an individual tailored therapy. In the same time, the modern diagnosis, based on imaging methods and molecular techniques, represents a source of innovative exploration pertinent to the acknowledgement of fundamental mechanisms of diseases.State-of-the-art imaging methods used for the assessment of digestive diseases currently include PET-CT (Positron Emission Tomography combined with Computed Tomo-graphy), MRI (Magnetic Resonance Imaging), as well as several endoscopic methods: Autofluorescence Endoscopy Imaging (AFI), Laser-Induced Fluorescence Spec-troscopy (LIFS) and Confocal Laser Endomicroscopy (CLE). Modern diagnosis based Studiu de fezabilitate – TARGET SF 6

on cell biology and biochemistry methods is oriented to elucidate the molecular mechanisms of clinical pathology, with the aim of identifying new molecular targets for testing new drugs. Laser microdissection and confocal laser microscopy are two new techniques used for this approach. In the analytical field several techniques recently became routine in the molecular investigation laboratory. These include the identification of tumor markers (Matrix Array Laser/Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) and Atomic Force Microscopy (AFM)), as well as the identification of gene groups which can characterize a cancer type collectively (DNA microarrays, Genomics). The actual research focus is placed also in the field of information technologies in order to create human-machine interfaces. The best example is the recent creation of artificial neural networks controlled by bio-currents derived from human brain.Nowadays, digestive pathology and especially digestive cancers are a major health problem in the world. These cancers are in the first place in the tumor pathology, with over 3 million patients and 2.2 million death people every year. Thus, digestive tract cancers are a major cause of death, having an incomplete response to chemotherapy in advanced stages and usually a very poor prognosis. The most frequent cancers in Romania are: colo-rectal cancer, gastric cancer, esophageal cancer, liver and pancreatic cancer, all being curable if diagnosed in early stages. These types of cancer are presently treated by multimodal treatment, including surgery and radio-chemotherapy, while new therapeutic strategies are still under evaluation. The latest discoveries in the tumor biology have identified some vulnerable therapeutic targets and created new therapeutic agents. The treatment strategies of advanced digestive cancer patients recently involved therapeutic combinations between cytotoxic and anti-angiogenesis agents, as a new and attractive target in the tumor therapy, which can keep under control the tumor progression for a long time.The use of several modern imaging techniques in the gastroenterology field has revolutionized the management of patients suffering from digestive diseases, as well as the early detection of digestive cancers through screening programs oriented to high-risk group patients. Several imaging procedures (mainly PET-CT and MRI) have also developed strongly, leading to an increased accuracy of the diagnosis, as well as the precise staging of the digestive cancers, in order to improve the therapeutic protocols. Gastrointestinal endoscopy has also benefited strongly from new image processing methods (AFI, CLE, etc.) in the past 2 years, yielding higher diagnosis accuracy, a better detection rate of the structural changes and improved techniques which allow the detection and quantification of vascularization and neoangiogenesis. Currently, the diseases are defined through the identification of morphologic characteristics detected by imaging and/or microscopy. Although the diagnosis precision and accuracy can be improved by a better training in pattern recognition, the future target would certainly be represented by the development of “optical biopsies”, that means recognition systems which can detect cancers in real-time. Moreover, by identifying new characteristics and prognosis markers it will be easier to stratify patients according to the disease stage. The high-performance and high-resolution imaging methods mentioned above will help to improve image quality, with a subsequent progressive “computerization” of medicine, while several IT methods will surely help doctors to improve the diagnosis precision. Computer simulations will thus become essential in the learning process and in the evaluation of competencies. The research efforts of this project will make possible the creation of “intelligent imaging systems” with large memory databases which will automate many aspects of the non-invasive diagnosis techniques. Establishing an early diagnosis through complex genomic and proteomic techniques that are able to detect the molecular changes (such as the DNA mutations and the genetic expressions, as well as the codified


protein expression), will certainly support the progress of therapeutic management, especially in the case of digestive cancer patients. In conclusion, early cancer diagnosis will become more accurate and more efficient through the use of these new imaging systems. The amount and type of services that the medical system offers to the population depends on the refunding system, while both of them are showing a significant deficit in Romania by comparison with the European Union. This difference is certainly more striking in Oltenia region, where the resources are restricted as compared with other regions of our country. This is the main reason why a series of new techniques which are strictly necessary for research, development and innovation, but also for improvement of the population health status have not been introduced in Romania yet. Romania lacks the infrastructure necessary to initiate high-performance imaging examinations. Thus, both research and development, but also current medical practice will progress significantly by introducing early detection and screening programs, as well as recent diagnosis methods which use imaging, pathology, immunology or molecular biology techniques. The final objective will be to lower the morbidity and mortality induced by the late diagnosis of advanced digestive cancers. We are talking about the imaging techniques recently introduced in most of the important centers from abroad, including “state-of-the-art” imaging, endoscopic and interventional methods. By the introduction in Romania of a high-tech system based on positron emission tomography (PET) combined with spiral computer tomography (CT), it will be possible to establish a location through PET, while CT will enhance the correct anatomic diagnosis. A 3 Tesla magnetic resonance imaging (MRI) system will be also used to obtain high-resolution images of the digestive organs, including MR virtual colonoscopy and MRCP (Magnetic Resonance Cholangio-Pancreatography), as well as through the inclusion of magnetic resonance spectroscopy. Among the recent endoscopic methods, we can mention: AFI, LIFS, magnification chromoendoscopy (including NBI mode) and CLE. Endoscopic ultrasound (EUS) allows supplementary the staging of malignant lesions identified through some of the “red-flag” techniques mentioned above. Development of EUS examinations using several techniques (contrast-enhanced EUS, elastography, 3D reconstructions, etc.), as well as improvement of hybrid imaging systems (CT-EUS and possibly MR-EUS), will push forward the medical research, as well as the medical system to a level comparable to those of other EU countries. By combining these endoscopic techniques and by making comparative studies with other imaging techniques, it will become obvious that they are very important in analyzing the structure and the vascularization of the pre-malignant and malignant lesions. The introduction of these early diagnosis techniques will also benefit from the introduction of mini-invasive experimental therapeutic procedures of natural orifice transluminal endoscopic surgery (NOTES). Moreover, the concentration of these imaging methods in the same center will certainly allow the development of diagnostic and therapeutic hybrid methods (PET-CT, EUS-CT, EUS-MR, etc.), in combination with other complex methods of image analysis, based on artificial intelligence techniques (AI). This will highlight the decision making process based on precise diagnosis algorithms supported by evidence-based medicine. Several “state-of-the-art” endoscopic methods will be combined in Romania for the first time with PET-CT and the 3T-MRI, allowing interdisciplinary research in digestive tract cancers. Also, the PET-CT system as a gold-standard of gastroenterological oncology imaging will be unique in Romania. Several other endoscopic procedures (AFI, LIFS, etc.) will also be performed for the first time in Romania.

The techniques and the systems necessary for an early diagnosis based on pathology (immunohistochemistry and imunocytochemistry, in situ hybridization, laser capture


microdissection, two-photons laser microscopy), molecular biology (real-time PCR, high density microarrays, CGH, etc.), and biochemistry (MALDI-TOF-MS, AFM, etc.) are scarcely available in Romania or they do not exist at all in the Oltenia region. For example laser capture microdissection (LCM) and confocal laser microscopy will be used for the first time in Romania. The introduction of modern pathology (including the immunological investigation) at the level of an integrated module, which subsequently offers the necessary material and integration with the genomic and proteomic modules, has an absolute priority, because it is not currently utilized properly in Romania. The modern pathology diagnosis has registered great progress toward specific cellular identification through the use of dedicated antibodies for research and diagnosis. In particular, the panel of antibodies used for cancer diagnosis is continuously growing. The cancerous tissue is heterogeneous and the latest research results have shown that a correct diagnosis entails the simultaneous visualization of 3-4 antibodies. Great progress has been reached by the introduction of the two-photon laser microscopy, which, apart from the simultaneous detection of several antibodies, allows the observation of the dynamics of tumor markers in the native tissue during a considerable period of time (3-4 weeks). The confocal laser microscopy facilitates three-dimensional reconstructions and this offers a spatial image which corresponds to the native tissue morphology. For instance, the laser microscopy offers three-dimensional images of the recently formed vessels (neoangiogenesis), this being an essential process in the malignant tissue evolution. The two-photon laser microscope will be unique in our country. A lot of contradictory results are obtained in medical research because of tumor tissue homogenization and biochemical analysis. This is because of the heterogeneity of the tumor tissue which gives a different weight at the final result. This is why it is necessary to use a cryomicrotome to cut the tissue and a laser capture microscope for the microdissection. The isolated cells are then taken and analyzed by real-time PCR and the gene expression platform. Another approach is the molecular analysis through biochemical methods of the material obtained from cancerous cells. The last 5-years of research have shown that each cancer type is characterized by the expression of 200-300 genes. These genes have led to the current “genetic signature” concept, which is particular to each type of cancer. These genes are likely to constitute very soon the basis of the molecular diagnosis, which will probably entail a new classification of the cancers. The Genomic platform (DNA microarrays) will be used in order to identify these groups of genes which together characterize a type of cancer. However, it is also necessary to validate and quantify the genes identified through “Genomics” by real-time PCR (RT-PCR). Again, the Genomics platform will be unique in Romania. The genetic analysis has registered so great a progress, that it has been automatized. The automatization of separation and identification of processes allowed the human genome to be sequenced and this is a great achievement of this century. At the same time, we have to be aware of the fact that the gene is only one piece of information, while the protein is the effector. The automatization of the proteins’ separation and identification is extremely difficult, as proteins are very heterogeneous structures. Despite these difficulties, the proteome’s analysis has registered great progress, too. Apart from improvements made in the classical bi-dimensional electrophoresis domain, great progress has also been registered by the use of new Protein-Chip (by analogy with the DNA chips) for protein separation. The tumor markers can then be identified through MALDI-TOF-MS. This will be a unique technique in Oltenia.


Cells cultures will be used for in vitro caracterization of malignant tissue by sampling cells groups or individual cells. These cells are transferred for molecular study. In vitro cultures are very important models in the cellular, biochemical and molecular characterization of potential therapeutic targets. The laboratory model also offers us information on the evolution in molecular terms of the malignancy process. It represents the first step in creating new anti-cancerous medicines. To achieve these aims we will use techniques such as the two-photon scanning laser microscopy, which is highly recommended to phenotype and study cellular dynamics as it does not bleach the cells and it thus allows a long term observation, as well as AFM microscopy. Both techniques will be uniques in Oltenia.The use of the information society technologies in the intelligent systems field (like artificial neuronal networks, evolutionary algorithms, vector based machines, clustering algorithms, classification and decision trees, etc.) is only at the beginning in the world, especially in the imaging data study domain for the automatic diagnosis. This is the consequence of the complex processing of the data afferent to the medical imaging by Artificial Intelligence means, as well as of the weak connection between the IT specialists and doctors. The purpose of this project can become a breaking ground if it is put into practice in real-time. Moreover, the financial costs of this component of the project are relatively low, referring to relatively cheap IT systems, bibliographic references (specialized books and articles) and software for the image processing. The role of the AI module is especially to optimize currently known algorithms, as well as to develop new types of algorithms for intelligent systems, especially designed to process digital images in this field. Consequently, the costs of procurement of highly specialized software will be significantly diminished.The early diagnosis of digestive cancers will consequently lead to a significant decrease of the morbidity and mortality, but also to a consequent decrease of futile surgical interventions, by the exact selection of the patients groups and also by the diminution of the high costs determined by the incomplete and deficient management of the patients. The quick identification of the presence or absence of the disease risk and of the preneoplastic or neoplastic lesions (in situ early cancer or invasive advanced cancer) will lead not only to the early diagnosis, but also to the potentially curative treatment of the lesions detected in real-time. The identification of the prognosis markers and the sub-division of patients by risk groups will allow an improvement of diagnosis algorithms, with a consequent increase of the efficiency of the medical decisions and the survival of oncological digestive patients. This project comes to solve the problem of lack of equipment that is necessary for the research and development activities, by creating a new Excellence Center in Gastroenterology as basis for the improvement of the University of Medicine and Pharmacy of Craiova research and development (R&D) infrastructure by means of some very modern equipment and software. By putting this project into practice, the infrastructure of a reference regional cancer center will develop in the Oltenia region, having as a consequence the growth of the research activity’s quality and efficiency in the south-east of Romania. The research center TARGET will focus on finding new strategies in the early detection of cancers by analyzing various cost-efficiency models and by improving the present strategies through the selection of the best screening methodology in accordance with evidence based medicine principles. By supplying this centre with equipment in accordance with the European Union standards, it will reduce the gap currently present in the south-west region of Romania, by developing the infrastructure of the University of Medicine and Pharmacy Craiova, in concordance with the 7th Framework Program’s thematic areas. This will clearly improve the programs used for the early detection and screening of cancer, this having direct implications in the


community medical assistance which will become a priority for the medico-social sector. The necessary equipment that is to be obtained by the present project will complete the actual resources of the Gastroenterology and Hepatology Research Center (authorized by the National University Research Council) and integrated in the national and international research structures.

Data of the entity responsible with project implementation Craiova is an old cultural and commercial centre, the most important city in south (after Bucharest) and offers an educational framework and cultural and historical also. The south-west area of Romania is one of economically under-privileged region. Thus, there are preoccupations and intensive efforts for the minimization of the existing gap. Over 2500 beds in 5 hospitals exists only in Craiova, thus the academic medical centre of Craiova assures medical assistance for the entire south-west region of Romania. In the framework of international research, UMF Craiova has a particular position having a direct frontier with two countries in Balkans – Serbia and Bulgaria, developing research programs in partnership with these countries universities and other Balkans or European countries as well. The University of Medicine and Pharmacy Craiova represents the only medical university in south-west area of Romania. The corresponding area has over 2.5 million inhabitants. In the framework of the University of Medicine and Pharmacy Craiova there are 4 faculties: General Medicine Faculty, Dentistry Faculty, Pharmacy Faculty and Midwifes and Nurses Faculty, with three specializations. The academic body has over 400 teachers and researchers which are training yearly 3000 students and 700 residents in almost all specialties. The students and the residents of our university are originally from various regions of the country, including regions with similar medico-pharmaceutical institutes. The entire academic body is integrated in highly esteemed research activity in our country and abroad.


http://www.hallo.ro/search.do?query=under-privileged

Modules Submodules

Support spaces

Laboratories

THEMES

2.b Investment description

2.b.1 The conclusions of pre-feasibility study or of detailed investment plan (if it was elaborated) regarding actual situation, the necessity and opportunity of investment promotion and technico-economic selected script as well

The scientific justification of investment The scientific justification has the role to support the necessity of investment. Thus, the research theme has the role to be the main element which justifies the investment. The TARGET project is scientifically based on four major research themes. These themes are, in fact, major research directions and the researchers of the TARGET center want to approach them after the project implementation. The research themes are related to the functional structure of the TARGET research center. Every theme will evolve within a precise module. Thus, the TARGET center will contain four modules (divided in submodules, while each submodule will be divided in individual laboratories and support spaces - you can see the next figure), every module with its own specific scientific destination.

NOTE!!! Each research theme will contain (after the project implemen-tation) specific research activities. These could be mixed up with the specific activities needed to create the research & development infra-structure, so they are consequenly named as “researches”.

For developing of the proposed “researches” included in the major research themes, we need a research infrastructure. This infrastructure has 2 investment components:

First investment component is due by equipments involved in the research flow. The technical specifications and characteristics of these equipments help us to find the optimal solution regarding to location (functional place).

Thus, we arrive to the second investment component: the construction of a building in B location and improvement of existing laboratories in A and B locations, similar to technical specifications of the equipments.

o The construction will have special characteristics, ordered by technical specification of the research equipment and by the strict flow of research subjects.

o Two types of fitting out are available. First type, ordinary fitting out: wall painting, reconstruction of sanitation, lighting system, flooring. The second


type, special fitting out. This fitting out is necessary for the protection of researchers life, because in some laboratories it will be develop researches at gene level and DNA. The special fitting out are controlled by SR EN 12128 / April 2003 standard regarding risk and biohazard situations.

NOTE!!! In order to emphasize the links between the themes-“researches”-equipment-utilities (necessary for the operation) of the equipment-laboratories-spaces (corresponding to laboratories), we have presented the “Equipment Table” which can be visualized at the subchapter 2.b.3 of the feasibility study.

In the following pages we present all four research themes, the modules that belong to the research themes (with submodules and laboratories), the specific researches which the researchers of the University of Medicine and Pharmacy Craiova want to extend and the research flows in the framework of themes as well.

THEME I. ESTABLISHING AN EARLY DIAGNOSIS IN REAL-TIME AND / OR ASSESSING THE PROGNOSIS MARKERS BY “STATE-OF-ART” IMAGING METHODS, IN AGREEMENT WITH MODERN PATHOLOGY AND MOLECULAR BIOLOGY TECHNIQUESA series of complex imaging techniques will be introduced in Romania based on this project, including combined positron emission tomography and computer tomography (PET-CT), as well as 3T MRI, with MR virtual colonoscopy and MRCP. By including a magnetic resonance spectroscopy module (MRS), information about the tissue metabolites specific to some diseases will be further obtained. An important part of the project will be represented by the assessment of several hybrid imaging techniques resulted from the fusion of images obtained by different methods. Different PET-CT, EUS-CT and possibly EUS-MR hybrid imaging techniques will be explored, together with some complex image analysis methods, based on AI techniques (neural networks, evolutionary calculation, etc.), which will be very helpful for the decision making process based on medical diagnosis algorithms. By introducing in Romania a PET-CT system as the gold-standard used in oncological imaging, different types of cancer will be detected at the molecular level, while the post-treatment staging and re-staging of the patients will be possible with increased accuracy, in order to assess the efficiency of therapy. The imaging methods will include recent endoscopy techniques which allow the visualization of the digestive tract, by means of high-resolution techniques such as AFI, LIFS, magnification chromo-endoscopy (MCE, NBI), EUS, OCT, CLE, etc. By introducing these latest endoscopic techniques, there will be a lot of research topics which aim at finding a real-time pathology diagnosis, as well as the molecular characterization of the digestive tract diseases, in the diagnosis of metaplasia, dysplasia, premalignant lesions, as well as early diagnosis of malignant lesions. By identifying the prognosis markers (such as the assessment of neoangiogenesis), patients will be divided by subgroups, in order to improve the decisional process and the therapeutic strategies. The diagnosis and therapeutic endoscopic techniques will be experimentally improved by some transluminal endoscopic surgery procedures and by experimental laparoscopic surgery. The improvement of the NOTES experimental techniques (Natural Orifice Transluminal Endoscopic Surgery) will be extremely important to make some prospective studies and to develop some combined mini-invasive surgery techniques, which are useful especially to diminish the morbidity and the futile surgical interventions.


This research theme will be developed functionally within Digestive Imaging Module.

I. Digestive Imaging ModuleThis module has a powerful research, diagnosis and treatment character due to the acquisition of several advanced techniques (unique in Romania), in addition to the existing ones. This module contains 3 submodules:

I.1. The Imaging and Radiology Submodule (Imaging Department building)I.2. The Digestive Endoscopy SubmoduleI.3. The Endoscopic Surgery Submodule.

I.1. The Imaging and Radiology Submodule Complex imaging systems (unique in Romania) will be used in this submodule, such as the PET-CT or the 3T MRI systems, in combination with the conventional imaging systems, such as digital radiology. Positron emission tomography (PET) combined with spiral computerized tomography (CT), or the fusion imaging, is one of the most modern and powerful methods used to offer functional images with a clear medical purpose. The traditional methods of image creation (US, CT, MRI) reveal the anatomic relationship between organs, the morphology of systems, with enhanced high-resolution, high-quality and three-dimensional images. The main disadvantage is that it is not possible to establish if the pathologic process is malignant or benign unless abnormalities appear in the organ’s structure, dimension or shape. The exclusivity of the PET-CT investigation consists in the fact that it offers information on the organs’ anatomic structure, as well as on the tissue metabolism. The development of new radio-labelled substances with metabolic substrate and receptor ligands for the study of the cellular functions has extended the PET-CT molecular imaging clinical applications, by offering unique diagnosis information that cannot be obtained by the conventional CT or MRI imaging techniques. By developing the new positron radio-agents with single-photon emission, including labeling of some enzymes, peptides, drugs and other antibodies, the medicine is now in the unique position of using the molecular mechanisms which stand as the basis of the pathological processes. Consequently, the apoptosis and neoangiogenesis imaging has been thus translated form the laboratory to the clinical practice. The fusion PET-CT imaging is applied at a large scale in the developed EU countries, offering diagnosis priorities in the molecular detection of different types of cancer, in the staging and re-staging procedures, and in assessing the chemo-radiotherapy effects. The procedure is being unanimously accepted as a gold standard in the oncological imaging, including gastroenterological oncology. MRI also has a growing role in the assessment of a large number of abdominal diseases, especially for pancreas, bile duct and digestive tube evaluation. The recent technical progresses regarding the magnetic field (including the large availability of 3Tesla MRI systems), but also improvement of the hardware and software systems allowed the acquisition of MR images which offer excellent anatomic details and do not present secondary artifacts determined by intestinal peristalsis or respiratory movements. The use of rapid sequences has reduced the acquisition time improving the patient’s acceptance and allowing the more efficient use of the system. The new three-dimensional sequences allow the rapid acquisition of images, reducing the defective registration of sections and movement artifacts, while the multiplane reconstruction is improved. The MRI which offers anatomic and functional details is continuously developing, with new techniques, such as the


diffusion and perfusion imaging are being evaluated. Further technical progress will offer wide uses of the MRI in the abdominal pathology. The optimization of sequences (for instance RARE, FISP) has put into practice a high-quality MRCP and has become an important non-invasive method by which the pancreatico-billiary system is being assessed. The dynamic images obtained from a combination of intravenous contrast agents and gradient-echo or fast breath-hold three-dimensional acquisitions have assured a more precise diagnosis of the abdominal vascular diseases. The 3D breath-hold acquisitions offer a precise diagnosis of small liver tumors. Recent studies have proven that MRI can be used as an imaging method in virtual colonoscopy, instead of using CT, as it offers a more precise diagnosis in intestinal diseases, without increasing the irradiation risk.

Romania is one of the few East-European countries where PET-CT or 3T MRI equip-ment is not used for the current moment and, therefore, the Romanian patients suffering from digestive affections are deprived of an efficient diagnosis which allows the detection of the disease in early stages, therefore reducing the medical costs and increasing the life expectancy. Moreover, the medical research has not benefited fully of these state-of-the art imaging systems which allow the integration of clinical-applicative information with fundamental data. The Radiology and Imaging Submodule will include three laboratories, with the following ”researches” that could be developed in connection:

I.1.A. Fusion Imaging Laboratory (PET-CT) Research a.1: Assessment of the usefulness of the PET-CT for the detection of

different digestive cancers at molecular level; Research a.2: Assessment of the usefulness of the PET-CT for the pre- and post-

therapeutic staging and re-staging of digestive cancers; Research a.3: Testing of PET-CT feasibility for the assessment of tumor apoptosis

and neoangiogenesis in patients with digestive cancers; Research a.4: Assessment of PET-CT as compared to 3T MRI for the assessment of

digestive cancers;

I.1.B. Magnetic Resonance Imaging Laboratory (MRI) Research b.1: Assessment of the role of high intensity magnetic field (3T) MRI,

combined with contrast agents and 3D reconstruction, for the early detection and characterization of the digestive diseases;

Research b.2: Assessment of MRI accuracy for the implementation of modern imaging techniques, including MRCP, virtual colonoscopy, MR spectroscopy, with a role in the early diagnosis of malignant digestive diseases;

Research b.3: Assessment of the feasibility of hybrid methods like EUS-MR, EUS-CT, for the early diagnosis and accurate staging of digestive cancers;

Research b.4: Establishing of a multi-step protocol of modern diagnosis of digestive diseases, which will stand at the basis of the medical diagnosis algorithms;

I.1.C Digital Radiology Laboratory (DRX) Research c.1: Assessment of the utility of digital radiology for the detection and

characterization of digestive diseases; Research c.2: Assessment of the utility of digital radiology for guiding ERCP and

miniprobe EUS catethers inside the bile duct and pancreatic duct; Research c.3: Assessment of the utility of digital radiology in setting stents in

malignant obstructive pathology of digestive tract; Studiu de fezabilitate – TARGET SF 15

Research c.4: Comparative assessment of ERCP (endoscopic cholangio-pancre-atography) and MRCP in the diagnosis of pancreatico-biliary pathology.

NOTE!!! Both the research themes and the main research categories justify the infrastructure and equipments needs.The connection between equipment-utilities (needed for proper functioning), of equipments-laboratories-rooms (afferent to the laboratories), and “researches”, can be identified at point 2.b.3 in the feasibility study.The researches will be identified by the above existing codes. For example: Research a.1: “Assessment of the usefulness of the PET-CT for the detection of different digestive cancers at molecular level” can be identified in the table by the code: ”R.a.1”

The following diagram plots the research flow which is planned in The Imaging and Radiology Submodule, as well as its connection to the other modules.

I.2. Digestive Endoscopy SubmoduleThis submodule will translate into practice some advanced endoscopic techniques which complete the existing ones: autofluorescence endoscopy imaging (AFI), laser-induced fluorescence spectroscopy (LIFS), magnification chromoendoscopy (MCE), including narrow band imaging (NBI), endoscopic ultrasound (EUS), optical coherence tomography (OCT) and confocal laser endomicrosopy (CLE). Parts of the necessary systems for this submodule have been already obtained during the unfolding of a recently funded national research project entitled: Platform of Interdisciplinary Research for Advanced Microendoscopic Imaging Devices (PYRAMID). The project has been financed in the PNCDI II competition in the Capacities program (2007-2009). The necessary equipment for the TARGET project thus completes the existing research infrastructure, by creating the bases of high functionality in research, as well as for professional interdisciplinary diagnosis.


The interdisciplinary character of these techniques will be ensured due to the close connection with other techniques (complex pathology staining, including immuno-histochemistry and immunocytochemistry examinations, as well as genomic and proteomic examinations of the biopsies and of the samples drawn through fine-needle aspiration – FNA biopsy). The possibilities of real-time pathology examinations of the endomicroscopic images will be assessed through advanced telepathology techniques.

The Digestive Endoscopy Submodule will include four laboratories:I.2.A. Endoscopic Autofluorescence Imaging Laboratory (AFI)

Research a.1: Assessment of autofluorescence endoscopy for the early detection of preneoplastic lesions (low or high-grade dysplasia), as well as esophageal or gastric adenocarcinoma;

Research a.2: Assessment of autofluorescence endoscopy for the colonoscopic screening of moderate or high-risk patients for an early detection of preneoplastic lesions (colorectal polyps) and colon adenocarcinoma.

Research a.3: Assessment of LIFS-type biopsy forceps for the real-time diagnosis of eso-gastric preneoplastic and neoplastic lesions;

I.2.B. Magnification Chromoendoscopy Laboratory (MCE) including NBI (narrow band imaging) mode

Research b.1: Assessment of magnification chromoendoscopy and NBI mode for the screening of patients with Barrett esophagus for an early diagnosis of esophageal adenocarcinoma;

Research b.2: Assessment of magnification gastroscopy and NBI mode for the screening of patients with preneoplastic gastric lesions and for detection of gastric adenocarcinoma;

Research b.3: Assessment of magnification chromoendoscopy and NBI mode for the colonoscopic screening of moderate and high risk, for the early detection of colon adenocarcinoma;

Research b.4: Assessment of accuracy of supervised intensive endoscopic training program;

I.2.C. Endoscopic Ultrasound Laboratory (EUS) Research c.1: Assessment of EUS accuracy (including contrast agents examinations

and 3D reconstruction for neoangiogenesis assessment) for the preoperative diagnosis and staging of patients with eso-gastric cancers.

Research c.2: Assessment of endoscopic ultrasound accuracy (including fine needle aspiration, contrast agents examination, EUS elastography, 3D examination) for the diagnosis and preoperative staging of patients with pancreatic cancer.

Research c.3: Assessment of EUS accuracy for the diagnosis and preoperative staging of patients with lung cancer (including complementary examinations of endobronchial ultrasound);

Research c.4: Assessment of the utility of hybrid methods, like ultrasound – computed tomography for the early diagnosis of hepatocellular cancer;

Research c.5: Assessment of the utility of hybrid methods, like ultrasound – computed tomography for the early diagnosis of pancreatic cancer;

Research c.6: Assessment of EUS-guided FNA for the early diagnosis of digestive cancers using immunocytochemical and molecular techniques (microarray, real-time PCR);


Research c.7: Comparative assessment of EUS elastography and ultrasound real-time elastography utility for the assessment of tumoral hepatic masses;

Research c.8: Assessment of accuracy of intensive training programs supervised by dedicated simulators. Development of the researchers diagnostic abilities through the use of simulators;

I.2.D. Confocal Laser Endomicroscopy Laboratory (CLE) Research d.1: Assessment of the utility of endomicroscopy for the in vivo and in real-

time pathological diagnosis in patients with Barrett esophagus and early esophageal adenocarcinoma;

Research d.2: Assessment of the utility of endomicroscopy for the in vivo and in real-time pathological diagnosis in patients with preneoplastic lesions and early gastric adenocarcinoma;

Research d.3: Assessment of the utility of endomicroscopy for the in vivo and in real-time pathological diagnosis in patients with ulcerative colitis and early colorectal cancer;

Research d.4: Assessment of the utility of endomicroscopy for the in vivo and in real-time pathological diagnosis in patients with Helicobacter pylori infection;

Research d.5: Assessment of the utility of endomicroscopy for the in vivo and in real time pathological diagnosis in patients with celiac disease;

Research d.6: Testing of the feasibility of endomicroscopy for distance transmission of in vivo and in real-time images for pathological examinations;

Research d.7: Testing of the feasibility of endomicrosopy for the assessment of tumoral neoangiogenesis in patients with digestive cancers (esophageal, gastric, pancreatic cancer);

NOTE!!! Both the research themes and the main research categories justify the infrastructure and equipments needs.The connection between equipment-utilities (needed for proper functioning), of equipments-laboratories-rooms (afferent to the laboratories), and “researches”, can be identified at point 2.b.3 in the feasibility study.The researches will be identified by the above existing codes.

The following diagram plots the research flow which is planned in the Digestive Endoscopy Module, as well as its connection to the other modules.


I.3. Endoscopic Surgery SubmoduleThis submodule will allow several “researches” for the development of mini-invasive surgery applications in the early diagnosis of malignant diseases. Endoscopic Surgery is a new surgery branch which aims at minimizing the ways of access in the peritoneal cavity in order to approach the abdominal organs. Wide incisions of the abdomen walls are thus avoided as they are unaesthetic, they extend the hospitalization post-operative recovery period and they induce the risk of post-operative infections or hernias. The first step has been made by the Laparoscopic Surgery (LS) by which the access in the peritoneal cavity is realized by small 2 cm orifices in the anterior or lateral abdominal wall.A more recent step has been made by the NOTES techniques (N.O.T.E.S. – Natural Orifice Transluminal Endoscopic Surgery). By this type of approach the access in the peritoneal cavity is not made by crossing the abdominal wall, but by the body’s natural orifices: oral orifice (transgastric), vaginal or anal (transrectal or transcolonic). The traumatisms of the abdominal wall, any parietal infection or subsequent hernias or aesthetic prejudices are thus avoided. A great benefit of Endoscopic Surgery (ES) by comparison with traditional surgery is the significant decrease of the general post-operative immuno-suppression thanks to the minimization of the parietal and peritoneal aggression. This represents an important advan-tage for the patients with malignant diseases, because the consequences of exploratory surgery, diagnosis or staging interventions should be minimal. Laparoscopic Surgery has attained a high technological level, while NOTES is only at the beginning, although its perspectives are unlimited. Endoscopic Surgery allows a direct visual, but also imaging (ul -trasound) exploration, while the biologic material drawn for biopsies has direct applications in the early diagnosis and staging of the malignant diseases of the digestive tract.

The Endoscopic Surgery Submodule will contain two interconnected laboratories, both both concerning the information flow, but also with the other modules (Digestive Endoscopy Submodule, Radiology and Imaging Submodule). Histopathological and immunohistochemical exams, cytological and immunocytochemical exams, but also complex genomic and proteomic exams of sampled biopsies or smears (sampled by fine needle aspiration) will be performed in the proximity of the submodule.

I.3.A. Experimental Surgery Laboratory (CEX) Research a.1: Assessment of the local immune response (peritoneal) modifications

induced by laparoscopic surgery; Research a.2 Assessment of the systemic immune response modifications induced

by laparoscopic surgery; Research a.3 Assessment of laparoscopic surgery influence on the biology of

secondary peritoneal malignant tumors; Research a.4 Assessment of the possibilities of staging of digestive cancers by

biopsy sampling and ultrasound examinations;

I.3.B. NOTES Laboratory - Natural Orifice Transluminal Endoscopic Surgery Research b.1 Assessment of the local immune response modifications induced by

NOTES; Research b.2 Assessment of feasibility and safety of transgastric approach in

NOTES; Research b.3 Assessment of feasibility and safety of transrectal approach in NOTES;


Research b.4 Assessment of various methods like peritoneal exploration, biopsy, ultrasonography for diagnosis and staging of digestive cancers;

Research b.5 Assessment of surgical oncologic resection in digestive cancers in NOTES;


The following diagram presents the predicted research flow in the Endoscopic Surgery Module and the connection to other modules.


THEME II. IMPLEMENTATION OF PATHOLOGIC DIAGNOSIS BY CONVENTIONAL AND MODERN METHODSThe conventional investigation methods (common and special stains) are relevant to the primary identification of the malignant pre-neoplastic and neoplastic processes. The immunocytochemical (ICC) and immunohistochemical (IHC) techniques will make possible, in the second stage of the investigation, the correct classification of the neoplastic processes and the identification of some possible prognosis factors, as well as therapeutic targets. The in situ hybridization technique (ISH) is the first step to be taken in order to clarify the carcinogenesis mechanisms at the molecular level by detecting at the tissue level the cells which can express pro-cancerous genes. Pathology currently benefits from a variety of investigations and research techniques such as: computerized tissue and cell morphometry, laser capture microdissection and monolayer liquid cytology.The laser capture microdissection method (LCM), represents an important pivot connecting the histopathologic investigation of the Pathology Module to the molecular investigation of the Molecular Biology and Biochemistry Module. The method aims at isolating cell groups which have homogenous morphology and are specific to the malignant tissue. Isolation of an homogenous group of cells is essential to establish morphological markers that are specific to pathological diagnosis. Moreover, specific cell isolation will offer the biologic material necessary to identify cell markers which will afterwards facilitate the development of some therapeutic molecular targets. In order to quickly determine the diagnosis, it is necessary to have a cryomicrotome with which to cut the native tissue into thin sections. At the same time, the cryomicrotome is essential as it provides working material for the LCM. The morphologic investigation algorithm is completed by the immunologic evaluation based on modern techniques (flow-cytometry and ELISA). Thus, flow-cytometry proved to be efficient in the digestive diseases investigation by estimating the tumor proliferative activity in colorectal lesions (DNA’s polyploidy evaluation, DNA index), by estimating the cell proliferation in digestive cancers, by evaluating the percentage and the absolute value of the lymphocyte populations and as a therapeutic indicator in colorectal cancer patients or in determining carcinoembryonic antigen (CEA) in biopsies of suspect lesions. The immunologic exam by the ELISA method will complete the assessment of malignant cells population behavior and the response of immunological defense by determination of a large palette of tumor markers, important viral markers and cytokines secreted by lymphocytes subgroups. This research theme will be developed in the Pathology and Immunology Module.

II. Pathology and Immunology Module The Pathology and Immunology Module aims at creating the necessary conditions for the application of high-performance pathological, cytological and immunological diagnosis by means of modern techniques, as well as special stains. The techniques used will include: immunohistochemistry and immunocytochemistry, in situ hybridization (using the FISH and CISH techniques), computer analysis of microscopic images, flowcytometry and ELISA. The modern research and investigation arsenal of Cytology and Pathology, includes a great variety of techniques such as: the immunohistochemical staining technique, in situ hybridization (with fluorescence - Fluorescence in Situ Hybridization – FISH or with chromogen - Chromogen in Situ Hybridization - CISH), computer analysis of microscopic images, laser capture microdissection and single-layer liquid cytology.


This variety of methods ensures a correct and complete evaluation of pathology diagnosis which will further allow: - primary identification of non-tumoral, preneoplastic and neoplastic lesions;- correct classification of neoplastic lesions;- correct and complete assessment of the factors involved in oncogenesis;- correct and complete assessment of tumor development;- identification and evaluation of prognosis factors;- identification of possible therapeutic targets efficient in the treatment of cancer;- assessment of the response to therapy;Major research objectives and activities of this module will include : 1. Investigation of transcription factors (nuclear proteins necessary for the transcription of certain genes, some of them being tissue specific). The advantages consists in the high degree of specificity and the absence of diffusion phenomenon, as compared with the classical cytoplasmic, nuclear membrane and extracellular space markers. Moreover, because the location of the marker is inside the nucleus, immunoreactions can be combined with different chromogens addressed to cytoplasm or cytoplasmic membrane markers. 2. Evaluation of prognosis factors in different digestive cancer types (ki67, p53, PCNA, bcl2, VEGF, etc.). In malignant digestive pathology, for example, it was recently found the association between the over-expression of stathmin (major protein involved in microtubule depolymerization) and mutation of the p53 gene, both being correlated with tumor progression, poor prognosis and recurrence in carcinomas originating at the level of the digestive tract. Other examples consist in the over-expression of bcl-2, associated with a favorable prognosis in colorectal carcinoma, as well as the correlation of VEGF expression with tumor progression (as an indicator of recurrence and metastasis) in hepatocellular carcinoma.3. Assessment of DNA repair and apoptosis, as suppressor functions of tumor development. It is possible that the aberrant forms of certain genes controlling the apoptotic phenomenon (like BARD1 or p53), might not function correctly to suppress tumor development. Consequently, over-expression in the cancerous cells represents a marker of poor prognosis.4. Assessment of angiogenesis allows the tumors to restructure their own vascular support. Cancer cells cannot grow larger than 1 mm3, because oxygen and nutrient diffusion is insufficient in larger cell conglomerates. Due to the hypoxic or trophic stress, or as a direct result of oncogenic alterations, tumor cells produce angiogenesis signals, leading to the overexpression of genes that control the angiogenesis pathways, leading to a disorganized angiogenesis response. This dependence of tumors from the vascular flow offered by the endothelial cells favored the hypothesis of potential therapeutic strategies based on angiogenesis inhibition, which might lead to destruction of the tumor vascularization. This would further lead to a decrease of intratumoral perfusion, without the side effects of conventional chemotherapy.5. Detection of possible therapeutic targets in different types of malignant tumors. Thus, the receptor of tyrosinkinase, c-erb B2, is an important prognostic factor and an important therapeutic target in breast and gastric carcinoma. VEGF also represents an important therapeutic target in hepatocellular carcinoma. Detection of ciclo-oxygenase 1 and 2 receptors in the patients with colorectal polyps might initiate the treatment with NSAIDs to prevent the cancerous process and the possible evolution to colorectal carcinoma.


The algorithm of pathology investigation is completed through the integration of modern techniques of immunological assessment (flow-cytometry and ELISA). The major objectives and activities of this submodule include:(1) Demonstration of the applicability and utility of flow-cytometry for the investigation of digestive diseases through the evaluation of tumor proliferating activity in colorectal carcinoma (assessment of DNA polyploidy – DNA index), assessment of cell proliferation in digestive tract cancers (for example detection of nuclear antigen p105), assessment of lymphocytes population as a marker of therapeutic monitoring.(2) Evaluation of the immune response of the patients with digestive tract cancers will be completed by ELISA which allows the assessment of the activity of lymphocyte subpopulations involved in the humoral and cellular immune response through the serum detection of secreted cytokines (IL-1, IL-2, IL-6, IL-8, TNF-alpha, IL-10, IL-12, etc.), as well as through the detection of antibodies with different specificities.

NOTE!!! Due to the connections and research flows that exists between different laboratories, the ”researches” of the Pathology and Immunology Module will take place sequentially in the submodules, and not in distinct laboratories as the Digestive Imaging Module.

This module will include two submodules: the Pathology Submodule and the Immunology Submodule.

II.1. Pathology SubmoduleII.2. Immunology Submodule

II.1. Pathology Submodule

Will contain the following laboratories and support rooms:A. Histopathology Laboratory (HP)B. Immunohistochemistry and immunocytochemistry laboratory (L-IHC/ICC)C. Laser Capture Microdissection, in situ hybridization and cytology (MdL-CIT-

HIS) D. Computerized Image Analysis Laboratory (L-ACI)E. Telepathology - Telemedicine Room (TELMED)F. Laboratory for tissue preparation and documentation (PMDF)G. Research and Diagnosis Rooms (CDD)H. LAN Secretariat / Servers (SS LAN)I. Materials and reactives storage rooms

The submodule aims to develop the following types of researches: Research p.1: The microscopic study of paraffin embedded samples and the

diagnosis of nontumoral or tumoral lesions by usual or special stauining techniques; Research p.2: The qualitative microscopic study of paraffin embedded samples and

the diagnosis of tumoral or nontumoral lesions using the immunofluorescence technique;

Research p.3: The qualitative microscopic study of paraffin embedded samples and the diagnosis of tumoral or nontumoral lesions using immunohistochemistry staining techniques;


Research p.4: The qualitative cytological microscopical study and the diagnosis of tumoral or nontumoral lesions by usual or special staining techniques;

Research p.5: The qualitative histological microscopical study and the diagnosis of tumoral or nontumoral lesions using the immunohistochemistry marking techniques;

Research p.6: Sampling of tissue and cellular material for study and diagnosis of tumoral or nontumoral lesions using histopathological and cytological techniques;

Research p.7: Sampling of tissue and cellular material for study and diagnosis of tumoral or nontumoral lesions using molecular pathology techniques;

Research p.8: The quantitative morphometric study of paraffin embedded samples or cytological samples and the diagnosis of tumoral or nontumoral lesions using computer analysis of imaging techniques;


The following diagram plots the research flow which is planned in the Pathology Submodule, as well as its connection to the other modules.


II.2. Immunology Submodule

Will contain the following laboratories:A. Flow-cytometry Laboratory (FLWC)B. ELISA investigation Laboratory (ELISA)C. The documentation, interpretation and data processing room (DIP)D. The sample receiving room (CPP)

The following researches will be performed within these laboratories: o Research i.1: The identification of characteristic nontumoral or tumoral

serologic and cellular markers using flow-cytometry; o Research i.2: The identification of characteristic nontumoral or tumoral

markers using the ELISA technique;


The following diagram plots the research flow which is planned in the Immunology Submodule, as well as its connection to the other modules.


THEME III. STANDARDIZATION OF THE GENE PROFILES IN THE DIGES-TIVE PATHOLOGY, IDENTIFICATION AND CHARACTERIZATION OF MALIGNANT PROGRESSION MARKERS AND THERAPEUTIC TARGETS BY MOLECULAR BIOLOGY AND BIOCHEMISTRY TECHNIQUESThe development and the evolution of some malignant tumor processes are followed by complex changes of gene expression. Although the individual genes were proposed for the diagnosis and were sometimes used as therapeutic targets for treating cancer, the last 5-year of research have shown that each type of cancer is characterized by the expression of 200-300 genes. These genes have led to the current concept called “genetic signature” which is specific to each cancer type. These genes are likely to become the basis of the molecular diagnosis, which will probably entail a new classification of cancers. Moreover, each gene of the “genetic signature” can be present in several forms, called polymorphisms. These groups of genes and polymorphisms which together are associated with an increased risk for cancer will be identified by means of DNA microarrays (Genomics). The microarray technique is an efficient and feasible method for getting and comparing the profiles of the genes’ expression from cancer patients to healthy individuals and those with premalignant lesions, as well as to patients with digestive malignant pathology. Some pattern of gene expression can become criteria of early diagnosis and could be very useful in the digestive lesions’ management. However, since the analysis of transcriptional activity using DNA Arrays is associated with false-positive results some genes must be eliminated. This disadvantage is inherent to the technique, as it has a statistic value. This is the reason why the DNA chip evaluation needs a statistic processing by means of specialized software in the Telemedicine and Artificial Intelligence Module (the Artificial Intelligence and Statistic Processing Submodule). The validation and quantification of the genes identified by “Genomics” will be done by real-time PCR (RT-PCR). Although the expression of genes offers a huge amount of information regarding the transcriptional activity of the cancerous cell, it doesn’t tell either what cells express that gene or any information referring to the genetic information translation in proteins at the cell level. Due to this reason three imaging and analytic platforms are necessary: LCM platform for the localization in the tissue of cancer related proteins, a platform for identification of relatively abundant proteins by MALDI-TOF-MS technique and AFM for analyzing of extremely low concentration proteins. The AFM technique can identify a large array of globular proteins (immunoglobulin, feritin, phosphorylase, phosphorylaskinase, member of P450 cytochrome, etc.) and complexes of these. Thus, in the framework of this project this method alongside complementary techniques as MALDI-TOF-MS and fluorescence methods (confocal microscopy) compiles the basis of a complete proteomic analysis platform for identification of biomarkers in malignant digestive diseases.

The malignant tissue is extremely heterogeneous. In order to make a phenotypic and molecular cell analysis of the cancerous tissue, it is necessary to isolate phenotypic homogeneous cells by the LCM method. By means of the laser microdissection we will realize:- (i) identification and quantification of important genes in the cancerous process, by isolating the messenger RNA and amplification by real-time PCR of cell groups specific to the malignant tissue; - (ii) verification of the tissue location of messenger RNA specific to the cancerous cell, by the ISH technique;- (iii) identification of the functional proteins by the MALDI-TOF-MS technique;


- (iv) investigation of the dynamics and of the topography of the proteins identified by proteomics at the cell substructure in native, physiologic state by using the AFM microscope whose resolution is comparable to the electronic one (nm / tens of nm).

In order to complete the study, we will realize: - (v) analysis of structural and conformational changes of the tumor markers by luminescence and spectroscopic studies. The AFM is a non-destructive method, while the spectroscopic changes can be used as diagnosis and prognosis criteria useful in the digestive disease management. - (vi) identification (phenotype) of the cells which express proteins specific to the malignant cells will be realized by two-photon laser scanner confocal microscopy (LSCM). This microscope can penetrate in the tissues even to 1-2 mm. Moreover, the bi-photon microscope can be used for observations of long time periods (weeks), so the cancerous process in an animal model can be kept under observation in vivo. The identification of these molecular and cellular markers will become the basis of new molecular therapeutic targets. For logistic reasons, the material used for the laser microdissection will be prepared by a cryomicrotome which allows cutting the native tissue into small sections. The cryomicrotome will be also used in the Pathology Module to establish a rapid diagnosis.New molecular cytogenetic methods will be added to the previous investigations. They do not entirely replace the classical cytogenetic techniques, but they can identify chromosomal changes undetectable by standard techniques (banding G, T, C, R). The new FISH method makes easier to characterize complex reshuffling of some chromosome markers, especially in tumor cells (gastric, hepatic or colorectal cancers). The comparative genome hybridization technique (CGH) will be used to establish the gene dosage and will be done with the equipment that will be acquired. This method is particularly useful to investigate the chromosomal aberrations in solid tumors where it is difficult to identify the karyotype.In order to further characterize the malignant tissue, cancerous cells will be cultured in vitro and used for molecular studies. Based on the model of cellular cultures, there will be studies on the intracellular activity, which will focus on the DNA replication and transcription, on the protein synthesis, on the cellular metabolism and on the cellular death (necrosis or apoptosis). It is important to establish relevant cellular lines for different tumors types in order to find therapeutic strategies to fight cancer.

This research theme will be extended within the Molecular Biology and Biochemistry Module. The activity of Molecular Biology and Biochemistry Module has mainly a fundamental research character. By acquiring new equipment in the framework of this module, besides the existing one, it will be possible to make better research in order to detect new diagnosis markers, but also to develop new training programs to familiarize the resident doctors and the candidates for a doctor’s degree with the latest international techniques. The techniques used in the framework of this module, together with the imaging and pathology techniques, will establish the interdisciplinary character of the research made within this center.


III. Molecular Biology and Biochemistry ModuleThe most important research activities will focus on:1. identifying transcriptional genetic activity profiles (Transcriptome) in the malignant gastro-intestinal pathology in order to establish a genetic diagnosis;2. quantifying of genes (gene dosage) which are specific to the cancerous cell by CGH;3. identifying some genetic mutations specific to the malignancy process;4. standardization of some biochemical tests for early diagnosis;5. detecting some possible therapeutic targets at the cellular and molecular level;6. determining some protein markers (tumor markers, cytokines, growth factors, fibrosis factors, oxidative stress markers) in biological liquids (serum, plasma);7. evaluating the specificity of some genetic and biochemical marker panels – as non invasive diagnosis indexes in colon, pancreatic, gastric and liver cancer;8. determining of proteic markers in tissue extract and the correlation to the obtained result by analysis of biologic liquid for the correct diagnosis of mentioned digestive cancers; 9. developing nanosystems for the separation of the proteins according to their biologic properties;10. installing molecular detectors able to “measure” individual or complex molecules, as the AFM microscope or Raman microspectroscope;11. phenotype characterization of the cancerous cells;12. corroborating the results of the biochemical determinations with the clinical and imaging diagnosis and their use as indexes of prognostic and of monitoring the efficiency of therapy.The aims of this module can be attained by means of new molecular technologies based on global analysis of the genetic expression: the microarray techniques doubled by real-time PCR, CGH and the DNA sequencing (the advanced optic system is currently being acquired), protein separation and analysis through advanced techniques (MALDI-TOF-MS, AFM and Raman).For example, the AFM is used to visualize a wide spectrum of globular proteins (such as immunoglobulin, ferritin, phosphorylase, phosphorylase-kinase, members of the P450 cytochrome family, etc.) and their derivatives. This method, together with complementary techniques such as MALDI-TOF-MS or fluorescent techniques like confocal microscopy, represents the basis of a complete proteomic analysis platform, their purpose being to identify biomarkers for the digestive tumor pathology. The activity of the Molecular Biology and Biochemistry Module will be aimed at:

imaging, creating and introduction of some molecular diagnosis tests in digestive cancer by using latest technologies (based on genomics and proteomics) and transferring the results from the laboratory to the clinic, in the patient’s benefit;

making studies in order to initiate a data basis of the proteins involved in cancer; the studies will refer to plasmatic and tissue proteome changes, in order to detect efficient biomarkers for an early diagnosis in digestive cancers, for prognosis and therapy monitoring, but also to establish optimum prevention and therapeutic targets;

evaluating the efficiency of some protein marker panels (tumor markers, cytokines, growth factors, fibrosis markers, etc.) as diagnosis indexes in digestive cancers, their use as prognosis markers and monitoring of the efficiency of the selected therapy;

establishing the identity of the malignant cells by phenotyping.

The researches will be performed in the following submodules:III.1. Molecular Biology Submodule


III.2. Biochemistry Submodule

III.1. Molecular Biology Submodule

This submodule will include the following laboratories and support rooms:A. Genomic Laboratory (LG)B. Cytogenetics Laboratory (LCG);C. Cellular Cultures Laboratory (LCC);D. Secretariat – The Analysis and Documentation Centre (SCAD);E. Sterilization Space (SS);

The following researches will be performed in the framework of these laboratories:

III.1.A. Genomic Laboratory (LG) Research g.1: Detection of microsatellite instability (MSI), single nucleotide poly-

morphism (SNP) and gene mutations involved in malignant pathology of digestive tract;

Research g.2: Assessment of methylation state and loss of heterozygosity state (LOH) in malignant tissues;

Research g.3: Simultaneous assessment of level activities of hundred of genes (microarray) followed by validation of results through Real-Time qPCR;

Research g.4: Generation of several profiles of genetic status for healthy subjects with premalignant or malignant lesions. Detecting of genetic markers useful in digestive cancers screening;

III.1.B. Cytogenetics Laboratory (CGL) Research cg.1: Initiation of cellular cultures out of lymphocytes and tumors for

chromosomal preparation; Research cg.2: Accomplishment of chromosomal preparations and the preparedness

for exam (banding, denaturation, hybridization, etc.); Research cg.3: Microscopic exam and karyotyping, results interpretation;

III.1.C Cellular Cultures Laboratory (CCL) Research c.1: Initiation of primary cellular cultures from cells and tumor tissue

sampling from patients; Research c.2: Stabilization of high-fidelity cellular lines reflecting tumor cells

characteristics; Research c.3: In vitro testing of potential therapeutic substances; Research c.4: Accomplishment of genomic and proteomic experiments on cells

cultures; Research c.5: Long and medium term storage of cell lines;



The following diagram plots the research flow which is planned in the Molecular Biology Submodule, as well as its connection to the other modules.

III.2. Biochemistry Submodule

It will include the following laboratories:o MALDI-TOF Analysis Laboratory (MLD)o Laboratory for Atomic Force Microscopy (AFM)o Laboratory for Luminescent Measurements (LL)o Laboratory for two-photon confocal microscopy (MCF)o Sample Processing Laboratory (LPP)

This submodule will also benefit of some spaces used for the purpose of a Data Analysis Centre (CAD), and for depositing materials and reagents (SD), as well as of a server room (SS). The following researches will be performed in the framework of this submodule:

III.2.A MALDI-TOF Analysis Laboratory (MLD) Research p.1: Obtaining of SELDI-TOF mass spectrum of plasmatic proteins in

healthy subjects; Research p.2: Obtaining of SELDI-TOF mass spectrum of plasmatic proteins in

suspected or diagnosed subjects with malignant digestive pathology;


III.2.B Laboratory for Atomic Force Microscopy (AFM) Research p.3: Analysis of data obtained in various conditions for the assessment of

the sensitivity and specificity of the method for the studied pathology as compared with other types of cancer;

Research p.4: Cluster analysis will be used to identify the proteomic profile correlated to high-risk patients or already advanced malignant disease;

III.2.C Laboratory for Luminescent Measurements (LL) Research p.5: Characterization of plasmatic proteins properties in malignant

digestive diseases by chemiluminescence measurement;

III.2.D III.2.D Laborator microscopie confocala cu doi fotoni (MCF) Research p.6: Research of dynamics and topography of the proteins identified

thorugh proteomics techniques, oriented on substructural cellular level in native status.


The following diagram plots the research flow which is planned in the Biochemistry Submodule, as well as its connection to the other modules.


THEME IV. USAGE OF INTEGRATIVE TELEMEDICINE METHODS BASED ON ARTIFICIAL INTELLIGENCE AND STATISTIC PROCESSING TECHNIQUESThis complex objective will be realized through the development of telemedicine techniques, based on the creation of databases with public and private access, as well as through the use of modern artificial intelligence (AI) techniques in the automatic learning field (Machine Learning), in order to create real-time intelligent automatic diagnosis systems for the computerized surveillance of the medical decision. The AI technologies envisioned in the project include: artificial neural networks, evolutionary algorithms, vector machines, clustering algorithms, and classification and decision trees. Moreover, the use of modern multivariate analysis techniques, multiple linear regression, survival analysis and statistical inference, used for the deep statistical analysis of medical data, will get them closer for the use of the information technology in order to get a complete and accurate computer-assisted diagnosis. This research theme will be developed in the framework of Telemedicine and Artificial Intelligence Module.

IV. Telemedicine and Artificial Intelligence ModuleThis module should represent the bridge which makes the connection between all the other modules of the project, in order to facilitate work and information exchange between the involved laboratories and disciplines, as well as with the whole world, by Internet and by other information integrative methods. The module is complex and includes a Telecommunication and Data Bases Submodule with public and private access, as well as an Artificial Intelligence and Statistic Processing Submodule which will function as an interface between the medical equipment and the data bases developed within the modules.The information flow is mainly oriented form the medical data sources (text recordings, static and dynamic images, etc.) to the Telemedicine and Artificial Intelligence Module in the process of data collection and storage in a global data basis. The new data will be processed offline in the Video Processing Laboratory and stored as a set of analytical parameters which will be statistically analyzed in the Statistical Processing Laboratory. All the data will be finally analyzed in the AI Laboratory in order to reach to the right conclusions. The final aim of the activity of the AI and Statistical Processing Submodule is the creation of the on-line processing flow of information. Thus, the integrated software package built on software modules corresponding to each intelligent system will be able to provide a real-time automated diagnosis as soon it has been supplied with the corresponding medical information.

NOTE!!! Due to the connections and research flows that exists between different laboratories, the ”researches” of the Telemedicine and Artificial intelligence Module will take place sequentially in the submodules, and not in distinct laboratories as the Digestive Imaging Module.

The following two submodules will be included in The Telemedicine and Artificial Intelligence Module:

IV.1. Telecommunication and DataBase SubmoduleIV.2. Artificial Intelligence and Statistical Processing Submodule


III.1. Telecommunication and DataBase Submodule Research t.1: Obtaining video images from the medical equipment (static and

dynamic images) and storing them in a mega database with private access; Research t.2: Developing advanced algorithms and some automatic programs to

process the video files in order to identify a set of utilizable parameters in the comparative and significant statistical tests, as well as in the intelligent systems;

Research t.3: Results presenting, case reports and storage of research results;

III.2. Artificial Intelligence and Statistical Processing Submodule Research v.1: Interpretation and classification of the information according to specific

statistic processing and to advanced AI techniques; Research v.2: Design of intelligent systems (neural networks, evolutionary algo-

rithms, classification and decision trees, support vector machines, clustering analysis) and the implementation of intelligent systems in medical data processing;

Research v.3: Creation of integrated software packages built from corresponding intelligent system which lead to the automated diagnosis by choosing the most accurate diagnosis based on the competition of component modules;

Research v.4: Developing virtual modeling techniques (such as 3D-reconstruction and virtual palpation), as well as distance diagnosis (telemedicine);


The following diagram plots the research flow which is planned in The Telemedicine and Artificial Intelligence Module, as well as its connection to the other modules.


The current situationIn conclusion, digestive tract cancers are a major cause of death, having an incomplete response to chemotherapy in advanced stages and usually a very poor prognosis. However, the use of modern imaging techniques in gastroenterology has revolutionized the management of patients suffering from digestive diseases, as well as the early detection of digestive cancers through screening programs oriented to high-risk group patients. State-of-the-art imaging procedures (mainly PET-CT and MRI) have developed strongly, leading to an increased accuracy of the diagnosis, as well as the precise staging of the digestive cancers, in order to improve the therapeutic protocols. Gastrointestinal endoscopy has benefited strongly from new image processing methods in the past 2 years, yielding higher diagnosis accuracy, a better detection rate of the structural changes and improved techniques which allow the detection and quantification of vascularization and neoangiogenesis.Presently, the diseases are defined through the identification of morphologic characteristic detected by endoscopy and microscopy. Although the diagnosis precision and accuracy can be improved by a better training in recognizing the lesions’ pattern, the future target would certainly be represented by the development of “optic biopsies”, that means recognition systems which can detect cancers in real-time. By identifying new characteristics and prognosis markers it will be easier to divide patients according to the disease stage. The high performance imaging methods mentioned above will help to improve images, while the computer methods will surely help doctors to improve the diagnosis precision. The computerized simulation will thus become essential in the learning process and in the evaluation of competencies. The research efforts of this project will make possible the creation of “intelligent imaging systems” with large memory databases which will automate many aspects of the non-invasive diagnosis techniques. Establishing an early diagnosis through complex genomic and proteomic techniques that are able to detect the molecular changes (such as the DNA mutations and the genetic expressions, as well as the codified protein expression), will entail the change of the therapeutic management, especially in the case of digestive cancer patients. The early cancer diagnosis will become more accurate and more efficient through the use of these new imaging systems. The amount and type of services that the medical system offers to the population depends on the refunding system, but both of them are showing a significant deficit in Romania by comparison with the European Union. This difference is certainly more striking in Oltenia region, where the resources are restricted as compared with other regions of our country. This is the reason why a series of new techniques which are strictly necessary for the improvement of the population health status have not been introduced in Romania (PET-CT, 3T MRI, endoscopic autofluorescence, etc) and Oltenia (confocal laser endomicroscopy, laser microdissection system, microarray platform, AFM, etc.). These techniques are strictly necessary to enhance the population health status, but also to increase the research capacity of the University of Medicine and Pharmacy Craiova.

The development of an interdisciplinary research center of this magnitude will help boost high-quality research in the region, by attaining and coagulating a critical mass of researchers able to develop different interdisciplinary and transdisciplinary research and development projects.


The current situationIn conclusion, digestive tract cancers are a major cause of death, having an incomplete response to chemotherapy in advanced stages and usually a very poor prognosis. However, the use of modern imaging techniques in gastroenterology has revolutionized the management of patients suffering from digestive diseases, as well as the early detection of digestive cancers through screening programs oriented to high-risk group patients. State-of-the-art imaging procedures (mainly PET-CT and MRI) have developed strongly, leading to an increased accuracy of the diagnosis, as well as the precise staging of the digestive cancers, in order to improve the therapeutic protocols. Gastrointestinal endoscopy has benefited strongly from new image processing methods in the past 2 years, yielding higher diagnosis accuracy, a better detection rate of the structural changes and improved techniques which allow the detection and quantification of vascularization and neoangiogenesis.Presently, the diseases are defined through the identification of morphologic characteristic detected by endoscopy and microscopy. Although the diagnosis precision and accuracy can be improved by a better training in recognizing the lesions’ pattern, the future target would certainly be represented by the development of “optic biopsies”, that means recognition systems which can detect cancers in real-time. By identifying new characteristics and prognosis markers it will be easier to divide patients according to the disease stage. The high performance imaging methods mentioned above will help to improve images, while the computer methods will surely help doctors to improve the diagnosis precision. The computerized simulation will thus become essential in the learning process and in the evaluation of competencies. The research efforts of this project will make possible the creation of “intelligent imaging systems” with large memory databases which will automate many aspects of the non-invasive diagnosis techniques. Establishing an early diagnosis through complex genomic and proteomic techniques that are able to detect the molecular changes (such as the DNA mutations and the genetic expressions, as well as the codified protein expression), will entail the change of the therapeutic management, especially in the case of digestive cancer patients. The recently development of new imaging systems tends to increase the acceptance and efficiency of cancers early diagnosis procedures. The amount and type of services that the medical system offers to the population depends on the refunding system, but both of them are showing a significant deficit in Romania by comparison with the European Union. This difference is certainly more striking in Oltenia region, where the resources are restricted as compared with other regions of our country. This is the reason why a series of new techniques which are strictly necessary for the improvement of the population health status have not been introduced in Romania (PET-CT, 3T MRI, endoscopic autofluorescence, etc) and Oltenia (confocal laser endomicroscopy, laser microdissection system, microarray platform, AFM, etc.). These techniques are strictly necessary to enhance the population health status, but also to increase the research capacity of the University of Medicine and Pharmacy Craiova.


The necessity and opportunity of investment promotion The necessityThe proposed objectives of the Research and Treatment Centre are in accordance to the general objectives of research programs in Romania, because they allow the development of applicable essential researching to prevent a diseases group with major impact; the development of essential medical science, the usage of medical information network with optimal administration of information included in an academic network, and also implementation of telemedical modern techniques, which allow the end of differences against European medical services level.According to expectations of this project implementation, it will be developed researching themes, whose results go firstly to acquiring an early diagnosis in malignant pathology, which would have direct influence over people’s health, especially in Oltenia, and generally in Romania.A growth of life quality (people’s health) will also have implications on economic environment in Romania.The project is emphasized because it puts the basis and creates an excellency centre in gastroenterology as a support to infrastructure CD development in UMF Craiova through endowment with the most modern equipments, instruments, software which contribute at the development of C-D existent infrastructure.By infrastructure development of the excellency centre, it will be possible to expand the international partnership in C-D (especially in European planning) and to develop technological ideas with economic interest potential for Romania through endowment and modernization of existent laboratories. The creation of a new researching infrastructure which integrates the 4 Modules described (Imaging Modules, Pathology and Immunity Modules, Molecular Biology Modules and Biochemistry, Telemedical and Artificial Intelligence) belongs to major intervention area, which will allow the increasing of C-D ability and efficiency activity of UMF Craiova.

The opportunity The participation possibility at European researching programs

After the implementation of TARGET project, University of Medicine and Pharmacy Craiova will have the possibility, due to researching infrastructure, to develop the project in partnerships with other institutions and CD institutes in Europe, for example the current Framework Program 7, where UMF Craiova can apply CD projects for Cooperation Program-Health Area.In the past there were international projects of UMF Craiova which, because of insufficient infrastructures, were rejected by the international assessors:1. „Mini-invasive Evaluation of tumour angiogenesis” Project was evaluated in Framework Program7 -Ideas, Call ERC-2007-StG, obtaining a score of 6.5 from a total of 10. The main reason which didn’t obtain the subsidiary was that of resources deficiency (infrastructure and human resource. Although, the project was considered very interesting by the assessors, with an important practical impact, it wasn’t subsidized.2. Researchers from Researching Gastroenterology and Hepatology Centre of UMF Craiova, tried to apply, in a consortium, for Framework Program 7 - Partnerships- the „Europhysione-Eu_Giome” project. Despite this, the consortium couldn’t be achieved because of insufficiency of resources and equipments (especially, because of the lack of some endowments like confocal laser endomicroscopy and endoscopic autofluorescence). It is desired that by achieving TARGET centre infrastructure, future projects would not be rejected because of infrastructure insufficiency.


The possibility of subsidizing through POS-CCE, prior axis II: competition through researching, technological development and innovation, major area of intervention 2.2 - Investments in CDI infrastructure, Operation 2.2.1: The development of existent CD infrastructure and creation of new CD infrastructuresArea Operative Program-Increasing of Economic Competition, through major Area of Intervention2.2. Operation 2.2.1. It is the only Area Operational Program which subsidizes the creation of CD infrastructures for institutions which develop CD activities.Certainly, we can say that there are programs either national (National Plan of Researching, Development and Innovation II: Abilities and Partnerships Program), or international (Framework Program 7: Cooperation and Abilities) which subsidize in a certain measure the development of CD-I infrastructure of CD institution (acquisition of CD equipments), but none of them subsidize the creation of infrastructures (construction of buildings for CD).In these terms we consider that it is convenient for the University of Medicine and Pharmacy Craiova, to apply for obtaining subsidizes from Structure Stocks in consideration of development of CD infrastructure.

The uniqueness of infrastructure makes that researching as those of 2.b.1. „Science justification of project” be possible:

o detection of molecular modification (at ADN mutation level and gene expressions), respective of modified proteins’ expressions;

o achievement of integrated imaging system;o initiation of screening programs and early detection with the following possible

consequences: decreasing of mortality and morbidity induced by digestive cancers;

o usage of ultramodern endoscopy techniques: autofluorescence endoscopy, spectroscopy with laser fluorescence, magnification cromoendoscopy with NBI mood, optical coherence tomography and laser confocal endomicroscopy;

o characterization in situ of malignant texture through usage of microscopy techniques with 2 photons;

o usage of informatic technologies of intelligent systems area (artificial neuronal networks, evaluational algorithms, cars with vectorial support, cluster algorithms, tomography with optical coherence and decision)- incipient techniques at world wide level;

o fast absence and presence identification of sickness risk, respective of premalignant lesions.

Creation of infrastructure can take to the following results:o early identification of digestive cancers with direct result over mortality and

morbidityo fast identification of presence or absence of sickness risk;o the integration in Researching European Program and the improvement of

researching potential inside the project will be ensured by constant collaboration between project participants and other gastroenterology departments, medical imagistic and medical informatics: Laboratory of Endoscopy and Department of Gastroenterology and Hepatology, University Hospital Aarhus, Denmark; Laboratory of Gastrointestinal Endoscopy and Department of Surgical Endoscopy, Gentofte University Hospital, Copenhagen, Denmark; University Group for Healthcare Modeling, University of Westminster, London, United Kingdom;


o integration of ultrasound endoscopy and endomicroscopy laboratory in formation, the network of European Society of Digestive Endoscopy (ESGE) will allow the formation of Romanian and foreigners experts inside researching centre, respective formation of Romanian experts in high reputation centre in Europe under the care of NEEG and ESGE. We mention that Endoscopy Laboratory of UMF Craiova disposes of performant equipment, inclusive through the gain of 2 CEEX grants in 2006 on which basis will acquire performant equipment of ultrasound endoscopy elastrography, the only one in Romania in this moment. Activity of Ultrasound Endoscopy Lab can be reflected through creation of a CD and an interactive and dynamic website of linear ultrasound endoscopy, www.EUSAtlas.ro, which already has some registered users from Romania and abroad (USA, France, Netherlands, Denmark, Sweden, Germany, Hungary, Mexico, Taiwan, Thailand, Vietnam, India, China, Venezuela, Egypt etc) over 18000 individual visits permanently contributing at dissemination results of personal researches and increase of clinic prestige, respective centre. The CD and website has been awarded with The Biggest Prize Gheorghe Badea, every year prize of Romanian Society of Ultrasonography in medicine and Pharmacy Biology, awarded at the second national Congress of Ultrasound, Cluj Napoca, May 28th, 2005. Moreover, the extinction of site with pictures and films of some new endoscopy mastrography or Endomicroscopy techniques would allow increasing of popularity and dissemination through this activity:

o large spreading of information through articles which will be published in extenso in ISI and/or Medline index magazines, through works which will be presented at national and international meetings, respective through achievement of continuous medical education materials (CD, DVD, sites, web).

Taking into account the strategic directions of Area Operational Program for Economic Competition Increasing which are in concordance with guiding lines proposed by European Committee, it is necessary and convenient to introduce this project because stocks of investment allocated to this research area were insufficient, and own sources entirely missed.

Selected technical-economic scenarioIt is proposed the implementation of TARGET project with the next components:

- Building construction It is desired the achievement of a construction in Location B (1st, May Bdv, no. 66, Craiova) with S+P+3E characteristics, with Hmaxx14,15m (compared to ground quote), with built surface of 445mp and built developed surface 2250mp.The construction will have a structural system made of BA: BA frames, BA beams and floors, and covering system with thermoisulating and hydroisulating terrace.The construction will have branchings at:- water feeding system;- sewerage system, used and domestic waters;- sewerage system, pluvial waters;- electric energy/telephony/internet system;- gas feeding system.


http://www.EUSAtlas.ro/

NOTE!!! You can find information about building at 2.b.3.+ Building Construction in Location B”

- Achievement of arrangements for existent Location A and B It is wanted the achievement of 2 arrangemet types:

special arrangement, for white rooms (in Location A, Petru Rares Street, no. 2, Craiova) regulated through standard SR EN 12128/2003, standard regarding security levels of microbiology labs, risk areas, situations and security demands. These arrangements will be achieved for labs of Molecular Biology Sub Module (SS, SR, SC, LLC, LCG, LG, CR) and for labs of Biochemistry Sub Module (MLD, LPP, SD, MCF).

normal arrangements- these ones (paintings, replacement of electric and thermal installations, parquet replacement, isolation systems, doors and windows) are necessary regarding an ensuring of an optimal average both research development and optimal functioning of research devices.

The arrangements will be achieved in the project’s Location A and B

NOTE!!! You can find information about arrangements at 2.b.3.-„Arrangement of spaces in Location A and Location B”

- Acquisition of equipments and furniture There are wanted acquisition equipments for the achievement of research -developing activities. Many of these equipments have „state of the arts’ character and they are the only ones in Romania. It will be acquired IT equipments too, necessary for achievement of integrated researches ( Telemedical and Artificial Intelligenge Equipments Modules).Both Building of Location B and buildings of Location A will be endowed with special medical furniture and normal furniture.

NOTE!!! You can find information about CD equipmentsIt equipments and furniture at 2.b.3-„Endowment of CD equipments”; „Endowment with hardware and software”; „Endowment with furniture”

2.b.2 Technical-economic scenarios

Technical-economic proposed scenarios:For the increasing of CD ability of UMF Craiova we can characterize the next scenarios:Scenario 1: Renting of some labs completely endowed in which UMF Craiova searchers can develop researching themes which are at the basis of scientific justification of TARGET project.

Scenario operabilityAn informal survey of the market in early 2008 revealed that approximately 350 to 400 3T whole-body–capable MR systems are currently operational in the world, with roughly 25% used primarily for research.Overall, the geographic distribution of cyclotrons throughout Europe, and thus the supply of 18F-FDG meant that development of PET/CT at the current rate could be sustained. While Germany, with 94 PET and PET/CT scanners, had the highest installed base of these


modalities in Europe, the major markets of the UK and France which are late adopters of the technology, were lagging far behind.Autofluorescence endoscopy and confocal laser microscopy systems represent the newest standard in gastroenterology. At the time, there is no autofluorescence endoscopy and only one confocal laser endomicroscopy in Romania. In Europe only 10 autofluorescence and 20 endomicroscopy systems are respectively functioned in highly ranking clinics.Despite the fact that these techniques and devices are absolute necessary in order to establish an early diagnostic related to pathology investigations (immunohistochemistry, in-situ hybridization, laser microdisection, two-photon microscopy), molecular biology (real-time PCR, high density microarray, CGH, ETC) and biochemistry (MALDI-TOF-MS, AFM, etc) they are very few (or even none) places in Romania where one can find them. In Oltenia region none of these techniques and devices can be found. It should be mentioned that both laser-microdisection technique and confocal laser microscopy are completely new for Romania.

Scenario incompatibility:a) The alternative of researches development in similar labs of study centers abroad is not viable because at this moment there are no study centers which broach only themes as those proposed.b) Not many explorers are disposed to go abroad to develop these researches.c) The costs of displacements are immense; University of Medicine and Pharmacy does not have the possibility of financial support of this type of scenario.

Scenario 2: The achievement of staged researching infrastructure, with help of internal and international subsidiaries. SCENARIO OPERABILITYStarting from premise that University of Medicine and Pharmacy Craiova can participate at PNCDI2 investigation national programs: abilities and partnerships, as well as investigation international programs, FP 7: abilities and cooperation, we estimate that, applying with smaller projects (from valuable point of view) for each o these programs, and obtaining maximum subsidies, it can be created an infrastructure similar to the one proposed through TARGET project approximately in 6 years and 9 months:

o PNCDI2: - Abilities Program-it is estimated the obtaining of a subsidiary with maximum value,

meaning 2.000.000 RON- Partnerships Program- it is estimates the gain of at least 3 researching projects

with maximum value of 600.000 RON, so a total value: 1.800.000 RONo FP 7:

- Abilities Program- it is estimated a gain at least of an approximately subsidiary: 3.600.000 RON

- Cooperation Program- it is estimated a gain at least of an approximately subsidiary:1.260.000 RON

Calculating, we can reach at the achievement of staged proposed infrastructure: with total of 8660.000 RON/year, we can reach at maximum investment proposed by this project, approximately 60.000.000 RON- in 6 years and 9 months.

SCENARIO INCOMPATIBILITY:a) None of these Programs (either national or international) subsidize construction of research infrastructure;b) None of these programs finances the acquisition of equipments with values that overtake 2.000.000 RON (aproximatively 540.000 Euros)


c) Time period necessary for infrastructure achievement is too big (6 years and 9 months).There is the possibility that proposed studies can not be brought up to date.

Scenario 3: Development of infrastructure of TARGET Research Centre

The creation of infrastructure supposes the construction of a building with special constructive specifications, achievement of normal and special arrangements for spaces which will lodge Modules research components of TARGET infrastructure, acquisitions of research equipments.From technical point of view, this scenario supposes the investment in:

construction of a building with the next characteristics: S+P+3E, Hmax 14,15 m (against ground quote), built surface 445 mp, developed built surface 2250 mp.

NOTE!!! You can find information about building at 2.b.3.+ Building Construction in Location B”

achievement of special and normal arrangements for Locations A and B- it is proposed the achievement of normal and special arrangements regulated through standard SR EN 12128/2003

NOTE!!! You can find information about arrangements at 2.b.3.-„Arrangement of spaces in Location A and Location B”

acquisition of CD equipments and furniture- it is proposed the acquisition of CD equipments, necessary for the development of studies and acquisition of specialty furniture

NOTE!!! You can find information about CD equipments and furniture at 2.b.3-„Endowment of CD equipments”; „Endowment with hardware and software”; „Endowment with furniture”

Scenario recommended by the assessor

Scenario 3: Development of infrastructure of TARGET Research CentreThe scenario recommended by lab supposes the construction of a new building which corresponds to the characteristics of research equipments (Location B), the achievement of special fitting out (for the white room) in labs from Location A and the achievement of normal fitting out for labs from Locations A and B.It is proposed the acquisition of CD equipments, IT and specialty furniture necessary for the endowment of a new construction in Location B, as well as the endowment of research labs existent in Locations A and B.You will find information about construction (construction memorial) about equipments and furniture at 2.b.3. - where we detailed the following:

- for construction – technical memorial- for equipments - minimum functioning characteristics, utilities necessary for good

functioning of the equipments, physical spaces intended to equipments’ placing and utility of equipments in researching flow.


Advantages of recommended scenario

period which we can achieve the infrastructure which doesn’t pass 3 years, against Scenario 2 where the achievement period of a similar infrastructure can start, if estimations remain inavariable, 6 years and 9 months.

it can achieve all structure in terms of subsidiary of the project through POS-CCE, Axis 2, Area 2, Operation1; in maximum 3 years

integrated development of propossed serching themes; creation of new working months in socio-economic unfavoured region Oltenia; the achievement of an infrastructure with „state of the art”, many of them unique in

Romania and even if South-East Central part of Europe; the possibility of some investigations in partnership with abroad research centers; the possibility that after implemetation of the project and development of researches to

obtain results with direct impact over the development of people’s healthcare and quality of poeple’s lives;

2.b.3 Constructuive, functional and technological description, in any case

Constructive description

Buildings construction in Location B

I. GENERALITIES

I.1 THE PROJECT ‘S OBJECT The present documentation, made according to provisions of Law 453/2001, contains the written and painted pieces for SF projection phase, and it refers to the achievement of construction works of Research and Imaging Centre UMF Craiova. According to P100/92, the building is framed in the second importance class. The importance category of construction is C. I.2 THEME DATAAccording to the projection theme, the documentation refers at the integral projection of the building. The functioning of the building is pavilion of labs for research activity.- Construction regime: Individual construction- Constructive system:

- Constructive system: frameworks in BA and floors in BA;- Covering system: - covering thermoisolating and hydroisolating terrace.

I.3 DATA REGARDING THE SITEThe lot, is situated in Craiova, 1st May Bdv, no.66- judicial regime: private property.I.4 ACCESSES AND CIRCULATIONS- auto access on lot: directly in the street- pedestrian access: directly in the street-stationary circulation: solved inside the lot (parking places on the lot)I.5 HEIGHT REGIMEThe existent building is framed in height regime of the area, being a construction: S+P+3E with maximum height at cornice: Hmax=14,50m (against ground quote).



II.THE DESCRIPTION OF ARCHITECTURAL SOLUTIONSII.1 FUNCTIONAL ORGANIZATIONMajor functioning scheme contents:- spaces for research activities (labs),- spaces for the administration of the buildingII.2. SURFACES OF THE CONSTRUCTION SC = 445 mp SCD = 2250 mpII.3 COVER CIVIL PROTECTIONAccording to the Ministry of Local Administration and Internal Order no. 602/2nd of December 2003, for the recommended construction is not necessary the precaution of a cover of civil protection.II.4 STRUCTURAL SYSTEM- constructive system: foundations in BA, frameworks in BA, beams and floors in BA;- covering system: thermoisolating and hydroisolating terrace.II.5 CLOSINGS AND DIVISIONSII.5.1 Closings - Curtain facade on aluminum structure- Opaque closings with veneering on ventilated structureII.5.2 DivisionsInternal divisions are made of walls with unstructured role of 12,5-25cm brick and easy gyps-cardboard partition walls with acoustic treatment of 15 cm.II.6 EXTERNAL AND INETRNAL FINISHINGSII.6.1 External finishingBase: decorative plaster and veneering with natural stone;Walls: Baumit type waterproof plaster, curtain wall on aluminum structure and veneering on ventilated structure;Joiner’s trade: aluminum doors and windows with termoisolating glass, provided with tearing of thermal bridge;Covering: termoisolating and hydrisolating.II.6.2. Internal finishingFloors: intense traffic moquette for offices, ceramics or natural stone for walls, sanitary groups and stairs, PVC floor-antistatic and antibacterial in labs.Walls: washable painting on gyps cardboard veneering and false divided ceiling.Joinery: wooden joinery (cellular plate doors (massive wood-furnishings), aluminum joinery with termoisolating glass).II.7 INSTALLATIONS ADHERENT TO CONSTRUCTIONThe construction is provided with elastic, sanitary installations, sewerage system, gas detached at town networks. The heating of internal spaces of construction will be ensured through own thermal station. The thermal station is placed at subsoil level and benefits of explosion surface according to P-118/1999.Installations:- feeding with water-branching;- sewerage system with used domestic waters- branching;- sewerage system with pluvial waters- branching;- electric/telephony/energy- branching;- gas feeding- branching.II.8 EXTERNAL ARRANGEMENTSThey are as following:- free spaces: sward and trees;- spaces for circulation: Ferro-concrete veneering;- spaces for pedestrian circulations: concrete pavements;Studiu de fezabilitate – TARGET SF 44

- spaces for gathering domestic garbage and residues resulted from technological flows: concreted ceiling etc.-surroundings: will be used those which exist.

III. CONSTRUCTION SITE ORGANIZATIONCONSTRUCTION SITE ORGANIZATION will be made in „flows in chain” system- the development of technological flows being the following:- construction works of infrastructure;- construction works of over structure- mounting workings of curtain facades, execution works of hydroisolatings;- execution workings of internal divisions;-joinery and finishing works.The waste resulted of construction works will be conveyed to the closest renting pit indicated by authorities (with written agreement of these).All construction site organization will develop on the lot, without being necessary other ground surfaces (neighborhoods and public area).

MINIMAL TERMS FOR QUALITY ENSURANCEIV. ENSURANCE OF QUALITY DEMANDS ACCORDING TO LAW NO. 10/1995IV.1 CONTROOL MINIMAL LISTIV.1.1 CLASSIFICATION OF JUDICIAL REGIME (GROUND, EXISTENT CONSTRUCTIONS)- GROUND: PRIVATE PROPERTY. IV.1.2 INVESTOR, INVESTMENT BENEFICIARY (USER), DESTINATION- investor: UMF CRAIOVA. - destination: RESEARCHING AND IMAGING CENTREIV.1.3.TECHNIQUE REGIMEIV.1.3.a. Accesses, circulation (auto), own parking insurance for visitors;- own parking for visitors too, are solved on the lot. IV.1.3.b. Alignment, retiring, height (number of floors)-there are respected the alignments provided in urbanism certificateIV.1.3.c. Utility insurance (electric, water, sewerage system, telephone etc)- the building will benefit of all utilities connected to technical edilitary networks existent into the areaIV.1.3.d. Integrated expressivity of the ensemble-all ensembles are framed in neighborhoods’ characterIV.1.3.e. Construction influence over environment (natural and arranged)- aren’t necessary major interventions over natural or arranged framework.- sunny/shadowing: there are respected provisions of Local Urbanism regulation.IV.1.3.f. Measures for protection against external pollutions- there is no external pollutions; the construction does not have pollution functions.IV.1.3.g. Radiation climate:- there is no radioactive emanation, electric, magnetic fields etc.IV.1.4 Modifications in flora, fauna- there are not necessary clearings, release of biologic agents.IV.1.5 Modifications in soil and subsoil (soil quality, slopes)- the soil doesn’t take place of fertility class I and II, in addiction, it is the situation within the built up area and it was definitive taken out of agricultural circuit;- release of natural ground is arranged for evacuation of pluvial waters to swearing system network existent in the framework of the lot.IV.1.6. Collecting evacuation mood- solid waste: town sanitation service;Studiu de fezabilitate – TARGET SF 45

- liquid waste: at swearing network which the area uses it.Through this project ensures quality demands provided in Law no. 10/1995 regarding the quality of constructions. In execution will be respected the solutions which are in the project as well as legislation and technical robustness prescription which regulates the execution of constructions- mounting works.

IV.2 SAFETY DEMAND IN EXPLOITATIONIV.2.1. Users’ safetyDocumentation provides floors (according to c37 normative), safety heights (according to STAS 6131) and utile heights (according to General Norms of labor Protection 1996)IV.2.1.a. External circulation- Concrete premanufactures with striation floors IV.2.1.b. Horizontal internal circulation- provided floors are antifire and non-skiddingIV.2.1.c. Vertical internal circulation:- stair in 2 steps according to 2h+1=62:64, approximately slope of 57%, width grade 1,20 mrail 90 cm- walking surface: steps are provided with striationsIV.2.1.d. Safety regarding aggressions from installations- electric installation: all under plaster, plugs and St switchesIV.2.1.e. Safety regarding maintaining workings-non-skidding floors (see internal finishing)IV.2.2. Construction security It is provided „The present purchasing of the construction’, according to Regulation approved through HGR 766/1997 and P130-88 Normative.Resistance and stability- structure insurance (frameworks in BA)- spatial organization is subordinated to structural traumaFire security demandProtection against neighborhoods:- distance between buildings: min. 10.00mProtection against fire conduction:-there are insured evacuations and saving ways: minimum width 1.50 m horizontal circulation, evacuation doors 1.00m etc.Intervention teams’ access:- firemen cars can intervene on existent road network and inside the building;IV.3 Hygiene and people’s health demand, remaking and environmental protectionIV.3.1. Possibilities for hygiene maintaining- internal finishing is washable;- evacuation of used waters to sewerage proposed network;- evacuation of domestic waste: outside the building in boxes for garbage disposed in some household platforms.

IV.3.2. Hygiene and people’s healthAll microclimate terms are ensured: temperature, humidity, natural and artificial lightning, natural and mechanical ventilation.Lightning, ventilation terms- lightning spaces: for all rooms are ensured the necessary terms of natural lightning (norm min. of 2h/day in winter solstice is more than satisfied) (and ventilation); spaces’ orientation respects RGU.- spaces’ ventilation: for all rooms are ensured the terms necessary of natural lightning; air volume computed: 2m3air/hour for 1 person.Studiu de fezabilitate – TARGET SF 46

IV.3.3. Remarks and environmental protectionThere are no pollution sources: water, air, soil. There aren’t pollutions. The local microclimate will improve itself through plantation of trees, bushes and grass on unoccupied construction grounds.IV.4 DEMAND OF THERMIC, WATERPROOF PREOTCTION AND ENERGY ECONOMYIV.4.1. THERMIC ISULATION:Climate terms:- temperature in winter: -15°- temperature in summer: +25°Constructive solutions and provided material ensure the thermal isolation- thermal bridges: avoidance (veneering with compact politer 5 cm).IV.4.2. Waterproof isolation- it is ensured with covers and closings (guaranteed by builder)IV.5 DEMAND OF NOISE PROTECTIONIV.5.1. Acoustic protection measures against the noise of building exteriorIV.5.1.a Spaces orientation:- spaces for research activities are oriented to sunny part.Insurance of air change in isolation terms against external noise is ensured through deficiencies (joineries).IV.5.1.b. Acoustic protection measures inside the building- division walls between functions on the same floor: brick joinery and cardboard gyps=mineral wadding cardboard gyps=15cm.IV.5.1.c. Spaces proposed to non-sound effects- spaces proposed to non-sound effects: offices and meetings and protocol rooms- spatial organization insures optimal terms for „isolation” of protected spaces- protected spaces: sound effect level computed max. 35 Db.The existent constructive structures are corresponding to building destination.Activities developed in exploitation do not impose special phonoisolating measures of closings and divisions.

V. OBSEVATIONSAt closing elements projection were respected the provisions of P118/1999 Normative.The necessary works to be executed and proposed technologies are useful to any contactor, for which reason they weren’t provided through special measures to necessitate additional charges.The builder, through service or representative with labor protection, will insure the execution staff by the terms which are necessary for the avoidance of working accidents or professional sickness. It will respect the norms of special labor protection of working place and operation which it executes in a certain moment respective workers, as well as Regulation regarding labor protection approved by MLPAT with Order 9/N/15.03.1999 according to Constructions Bulletin no.5/1993.It will be respected provisions of Law no.90 of July 1996.We mention that employed materials should have the characteristics provided in robustness standards, for which the bidder will present the technical arguments emitted by MLPAT-INCERC.The control of works’ quality will be made according to control program on finished phases.VI. GUARD MEASURES AGIANST FIRESThe documentation was made according to robustness PSI norms.The building is framed in the degree of fire resistance according to P118/1999 Normative.There will be respected the next norms:- projection and achievement of technical norms of constructions regarding protection at fire action, P118/1999 Indicative;Studiu de fezabilitate – TARGET SF 47

- general norms of prevention and fire extinction approved by MI no. 381/1994 - technical norms regarding the fireproof of the materials, wood and textile products used in constructions C58/1996 Indicative, approved by Order MLPAT no.24/NVII. MEASURES OF LABOR PROTECTIONDuring works’ execution will respect the provisions contented in robustness normative:– Law of labor protection no. 90/1996,– general norms of labor protection– 1996,NDPM 1968, NSPM 1969, NRPM 1975, NPM 1980 i.e.– Regulation regarding labor protection in constructions-MLPAT 9/N/1993For the insurance of lab protection during exploitation will provide protection rails against falling according to STAS 6131/73.This instructions are not limited, the builder at execution and the robustness beneficiary will take protection to additional measures of workers when they will be necessary, such as to avoid the production of accidents.

Spaces arrangement in Locations A and B

Special arrangements

For Molecular Biology Modules, which proposes to develop researching at gene and ADN level are necessary for the achievement of special arrangements (clean room), according to SR EN 12128/April 2003 Standard, the standard regarding the insuring levels of microbiology labs, risk areas, safety situations and demands, arrangements to insure both researchers’ life protection and optimal working average from the development of researching point of view.The next table presents the main arrangement operations and characteristics of arranged equipments.

Special arrangements I. Demolition of existent infrastructureII. Rearrangement of existent infrastructure and the endowment with:a. Equipment for acclimation ensuring parametersNr. Crt

Equipment Characteristics

1. Modular air – CTA treating central

-self-carrying structure, with missing surface;- air treated air: D=2, 7.000m³/h, available pressure dP=500Pa; - heating ability Qinc=30Kw for each primary agent T=65/55ºC;- cooling ability Qrac=16,30Kw

2. Double aspirate ventilator for evacuation and air recycling D=2

650m³/h; available pressune: dP=200

3. Absolute filters HEPA filter, efficiency class H13; 610x610x150 mm; 650x305x150 mm

4. Air introduction device of anode aluminum

Air conditioner on 4 directions, inclusive regenerating individual system; -450x450 mm

5 Aspiration and air recycling device

Bulk prefilter G4, inclusive regenerating individual system 300x600 mm

6 Humid air Adherent Air treating modular Central CTA; ensuring of


Special arrangements humidity level imposed at room level M=5,00kg/h; distribution degree of tubular system

b. Linoleum1 Antistatic Particles of 5µm/ft³: 650-100.000; 65-10.0002 Bacteriostatic3 Fungicide

This way they will need special arrangements for white room labs noticed in Spaces Schemata of Molecular Biology Sub Modules) visualization of the emphasized can be made at the following point „Endowment with CD equipments” with (SS, SR, SC, LCG, LCC, LG, CR, and in Spaces Schemata of Biology Sub Modules, with MLD, LPP, SD, MCF.

Normal arrangements

In the frame of normal arrangements will achieve paintings, the replacement of the floor, windows and doors, replacement of electric and thermal equipment.

Endowment with CD equipments

In the tables below (1st column) are presented the equipments of the 4 modules of TARGET research centre with submodules and labs.In the second column are presented characteristics of minimum functioning which have to execute the equipments. These characteristics correspond to the best researching need.The third column presents the utilities necessary for equipments’ functioning.The forth presents the link between equipments and functional spaces where they will be placed. In fact, after each table we will place relays to the functional spaces.The fifth column presents the link between equipments and their implication in researches. To visualize the researching types which are implied the equipments, please see 2.b.1. (the scientific justification) of feasibility study. It was wanted, through the achievement of this table, the demonstration of research need through correlation between researches (written below as „n Searching”), equipments necessary to research, utilities necessary for equipments to function, as well as functional spaces for equipments. We defined them like utility elements which an equipment/equipments system needs to function (e.g. number of people, electric energy type, natural gas, internet, soft and special programs etc).The creation of TARGET Researching Centre infrastructure, equipment acquisition and device for the labs proposed in this project, can determine the initiation of research themes proposed to be studied. The results of research activities can contribute certainly to diminishing financial losses, material and especially human. In the same time researching results can go to an increasing mood of output in many practical activities, they can be accounted. These researching themes propose them, respond the basis objective’s demand at Area Operational Program for Increasing of Economic Competition.The educational system in Romania is a great beneficiary of this project, such as students, masters and doctors can finish their studies in TARGET Researching Centre. After youth’s implication in researching we can provide an increasing of preparing and knowledge degree of young people in malignant digestive pathology area.Social exchange average is the biggest beneficiary; people in Unfavorable Area Oltenia can have access at diagnosis services and treatment in decent terms and with maximum efficiency.


I Digestive Imaging Module

I.1 Radiology-Imaging Submodule

I. DIGESTIVE IMAGING MODULEDevice/Equipment/System Specific requirements Utilities Location Area of

researchI.1 RADIOLOGY-IMAGING SUBMODULE

A. FUSION IMAGING LABORATORY (PET-CT)

1. PET-CT System with integrated positron emission tomography and computed tomography

a) PET system with multi-LSO-detector, crystal dimensions 4.0 x 4.0 mm, 70 cm gantry aperture, axial FOV 21.6 cm, 3D data acquisition and image reconstruction

- energy supply (220 V)- tap water- evacuation of biological and radioactive waste- air conditioning system

PET-CT

C a.1, C a.2, C a.3, C a.4

b) Multislice CT system (6 slices), 70 cm gantry aperture, adaptive array detector, 130 kV, 345 mA x-ray tube, extended 70 cm FOV, 3D image reconstruction, virtual colonoscopy software

- energy supply (220 V)- tap water- evacuation of biological waste- air conditioning system

PET-CT

2. Cyclotron System

An automated, compact negative ion accelerator, optimized for production and delivery of positron emitting radionuclides, self-shielding cyclotron

- energy supply (220 V)- tap water- evacuation of radioactive wasteevacuation - air conditioning system

PET-CT C a.1, C a.2, C a.3

3. Color Printer

Thermal dye sublimation print technique, 320 dpi resolution, output grayscale resolution 12 bits (4096 shades of gray), color resolution 16.t millions, maximum print sizes 24 x 30 cm, print throughput 100 films/hour, DICOM support and compatibility with PET-CT system

- energy supply (220 V)- air conditioning system

PET-CT C a.1, C a.2


B. MAGNETIC RESONANCE IMAGING LABORATORY (MRI)

1. Magnetic Resonance System (MRI)

Superconductive 3T closed bore - long (198 cm) magnet, 70 cm gantry aperture,102 integrated coil elements with 18 independent RF channels, 181 cm FOV, gradient field strength up to 45 mT/m, parallel image aquisition, total imaging matrix, virtual colonoscopy, MRCP and vascular analysis softwares

- energy supply (220 V)- tap water- wasteevacuation- air conditioning system

MRI C b.1, C b.2, C b.3, C b.4

2. Dry Laser Printer

325 dpi resolution, grayscale resolution 4096, dual scan, 70 films/hour, print sizes 35x43 cm, 28x35 cm, 35x35 cm, integrated DICOM interface, MRI compatibility


MRI C b.1, C b.2

3. Self-acting MR Injector for Contrast Media

Compatibility with 3T magnetic field strenght, multiphase programmable injection, flow rates 0.01-10 ml/s, dual syringe holder, 65/115 ml syringe disposables (Contrast Media/Saline),function prevents vascular occlusions (KVO)

- energy supply (220 V) MRI C b.1

C. DIGITAL X-RAY LABORATORY (DRX)

1. Digital X-RAY System

65-100 kW x-ray generator, dual focus rotating anode tube with high heat storage capacity and high thermal load capacity for small focal spots, 40-150kV tube voltage, exposing time 1 ms, tiltable from +90°/-17°, swivable tube assembly stand with rotation ±90°/-180°, storage digital system (2000 images), real-time acquisition and image postprocessing, integated DICOM interface

- energy supply (220 V)- tap water- wasteevacuation- air conditioning system

DRX C c.1, C c.2, C c.3, C c.4

2. Dry Laser Printer

325 dpi resolution, grayscale resolution 4096, dual scan, 70 films/hour, print sizes 35x43 cm, 28x35 cm, 35x35 cm, integrated DICOM interface, MRI compatibility


DRX C c.1, C c.2

You can find the plan with laboratories location in annex 8. Studiu de fezabilitate – TARGET SF 51

I.2 Digestive Endoscopy Submodule


researchI.2 DIGESTIVE ENDOSCOPY SUBMODULE

A. ENDOSCOPIC AUTOFLUORESCENCE LABORATORY (AFI)

1.Autofluorescence gastroscope + colonoscope

Autofluorescence and magnification capabilities (AFI, NBI, HDTV), compatible with the already acquired autofluorescence system

- power supply (220V)- tap water- evacuation of biological waste- air conditioning system

AFI C a.1, C a.2

2. LIFS- optic biopsy forceps (life induced fluorescence)

System console, with: laser, electronic fluorescent signal reception components, computer for analysis of tissue signal + biopsy forceps with built-in optic fiber, compatible with usage through the endoscope’s biopsy channel


AFI C a.3

3. Endobase - software and hardware for medical imaging integration

Image & video recording, DICOM + HL7 format, statistics, browser based application

- power supply (220V)- air conditioning system

AFI C1, C2, C3

B. MAGNIFICATION CHROMOENDOSCOPY LABORATORY (MCE)

1. High-resolution (HDTV) videoendoscopic system with NBI capabilities

HDTV and NBI modes; 1.5x magnification gastroscope and colonoscope, NBI compatible (narrow band imaging) and magnetic positioning system compatible


MCE C b.1, C b.2, C b.3

2. Plasma argon coagulation system

Superior and inferior endoscopy system compatible


MCE C b.1, C b.2, C b.3

3. 3D magnetic positioning system for colonoscope

Central unit for the detection of the colonoscope position, compatible with the colonoscope of the NBI system; real-time visualization of the colonoscope’s position


MCE C b.3, C b.4

4. Integrative Software and Hardware for medical images – Endobase type



MCE C1, C2, C3, C4


C. ULTRASOUND ENDOSCOPY LABORATORY (EUS)

1. Radial and liniar ultrasound endoscopy system, with contrast-enhanced EUS capabilities

Compatibility with the dedicated ultrasound system, with contrast-enhanced capabilities; 360 degrees radial ultrasound endoscope; therapeutic liniar ultrasound endoscope, with contrast enhanced harmonic EUS possibility (contrast-enhanced harmonic EUS)


EUSC c.1, C c.2, C c.3, C c.4, C c.5, C c.6

2. Dedicated ultrasound system with contrast-enhanced capabilities

Compatible with the dedicated radial and liniar ultrasound endoscopy system; 3D real-time; harmonic contrast low; extended field of view


EUSC c.1, C c.2, C c.3, C c.4, C c.5, C c.6

3. Radial and liniar ultrasound endoscope with elastography capabilities

Compatibility with the already acquired endoscopic ultrasound elastography system, 360 degrees radial ultrasound endoscope, liniar therapeutic ultrasound endoscope with elastography and contrast-enhancement capabilities


EUS C c.1, C c.2, C c.3

4. Dedicated ultrasound system with elastography and panoramic view capabilities

Real-time 3D imaging, advanced 4D ultrasound imaging technology, 2D and Spectral ultrasound technology, B/W and color panoramic imaging, Touch Elasticity imaging, contrast pulse sequencing technology, direct ultrasound research interface, full suite of array transducers, Hanafy Lens transducer technology


EUS C c.4, C c.7

5. Endoscopy and ultrasound endoscopy examination simulator

GI Mentor II, simulator for endoscopic gastro-intestinal investigations, ERCP, ultrasound endoscopy


EUS C c.8


image & video recording, DICOM + HL7 format, statistics, browser based application


EUSC c.1, C c.2, C c.3, C c.4,

C c.5


D. CONFOCAL LASER ENDOMICROSCOPY LABORATORY (CLE)1. Dedicated endomicroscopy system (HD digital video processor + gastroscope + colonoscope), in completion of already acquired system

Confocal in vivo laser endomicroscopy with x1000 magnification, with identification of tissue structures observed through conventional histology


CLEC d.1, C d.2, C d.3, C d.4, C d.5, C d.6

2. Endomicroscopy miniprobe system, compatible with the gastroscope, colonoscope and eneteroscope

Laser scanning unit (488 nm), micro and miniprobe (300 µm - 2.8 mm), variable length (2-6m), image processing software (Cellvizio GI)


CLE C1, C2, C3, C4, C5, C6




CLEC d.1, C d.2, C d.3, C d.4, C d.5

Hereby, in red, on the plan, are depicted the locations for the laboratories and accessory spaces for the Digestive Endoscopy Submodule



The Digestive Endoscopy Submodule locations include:

AFI = Autofluorescence Endoscopy laboratory (area 30 sqm) MCE = Magnification Chromoendoscopy laboratory (area 45 sqm) CLE = Confocal Laser Endomicroscopy laboratory (area 30 sqm) EUS = Endoscopic Ultrasound laboratory (area 30 mp) MON AFI MCE = AFI and MCE monitoring room (area 34 sqm) MON CLE EUS = CLE and EUS monitoring room (area 34 sqm) SS = Sterilization room (area 6 sqm) SA = Waiting room (area 21 sqm) SEDIU = Gastroenterology and Hepatology Research Center, main quarters (area 63 sqm) TMED = Telemedicine and Imaging room (area 21 sqm) DBSLAN = Database server and LAN room (area 16 sqm) ARHIVA = Multimedia archive (area 10 sqm) DEPOZIT = Imaging module storage space for media (area 7 sqm) MAGAZIE = imaging module storage (area 7 sqm) SECR = Logistics (area 43 sqm) WC = Toilet (area 26 sqm) Hallways (area 40 sqm)

I.3 Endoscopic Surgery Submodule


researchI.3 ENDOSCOPIC SURGERY SUBMODULE

A. LABORATORY FOR EXPERIMENTAL SURGERY (EXS)

1. Surgical lightingsurgical lighting system 150.000 lux, single spot, mount-ceiling

- power supply (220 V)- air conditioning system

CEXC a.1, C a.2, C a.3, C a.4,

C a.5

2. Anesthesia MachineAnesthesia machine – with closed breathing circuit

- power supply (220 V)- air conditioning system - oxygen supply

CEXC a.1, C a.2, C a.3, C a.4,

C a.5

3. Monitor Device to monitor the pulse and the blood pressure

- power supply (220 V) CEX

C a.1, C a.2, C a.3, C a.4,

C a.5

4. Pulse OximeterDevice for measuring the blood gases (O2, CO2) portable

- power supply (220 V) CEX

C a.1, C a.2, C a.3, C a.4,

C a.5

5. Video-laparoscopy system

TV monitorHigh-definition video camera Cold light sourceInsufflator – automaticElectrosurgery unit – monopolar and bipolarAspiration/Irrigation system

- power supply (220V)- tap water- evacuation of biologic waste- air conditioning system

CEXC a.1, C a.2, C a.3, C a.4,

C a.5

6. Instruments for laparoscopic surgery

Laparoscope 0 degree Laparoscope, 30 degree, Suction/Irrigation cannulaMonopolar dissection

- power supply (220V)- tap water- evacuation of

CEX C a.1, C a.2, C a.3, C a.4,

C a.5



researchhook; Unipolar scissors Metzenbaum; Bipolar scissors; Metzenbaum;Rotating hook scissors;Grasping forceps fenestrated 24 mm; Grasping forceps serrated 24 mm; Maryland dissector; Bipolar forceps

biologic waste- air conditioning system

7. Vessel sealing system System for vascular sealing


CEXC a.1, C a.2, C a.3, C a.4,

C a.5

8. Argon beam coagulator Argon beam coagulator


CEXC a.1, C a.2, C a.3, C a.4,

C a.5

9. Ultrasonic morcelator Ultrasonic device for cutting tissue in pieces


CEX

B. NOTES LABORATORY

1. Surgical lightning Surgical lightning 150.000 lux, single spot, mount-ceiling


NOTESC b.1, C b.2, C b.3, C b.4,

C b.5

2. Anesthesia machine Anesthesia machine with closed breathing circuit

- power supply (220 V)- air conditioning system- tap water- oxygen supply


C b.5

3. Monitor Device for monitoring the pulse and the blood pressure

- power supply (220 V) NOTES

C b.1, C b.2, C b.3, C b.4,

C b.5

4. Puls Oximeter Device for monitoring the SpO2l

- power supply (220 V) NOTES

C b.1, C b.2, C b.3, C b.4,

C b.55. System for videolaparoscopy

TV MonitorHigh-definition video-cameraCold light sourceAutomatic insufflator with automatic control of pressure Electrosurgery unit- monopolar and bipolar Aspiration/Irrigation

- power supply (220 V)- air conditioning system - tap water- evacuation of biologic waste

NOTES C b.1, C b.2, C b.3, C b.4,

C b.5



researchsystem

6. System for videoendoscopy

Trolly, Irrigation/aspiration system, cold light source, video processor, therapeutic gastroscope with ½ channels, therapeutc duodenoscope and colonoscope



C b.5

7. Argon beam coagulatorCompatible with systems for upper and lower endoscopy



C b.5





C b.5

Hereby, in violet, on the plan, are depicted the locations for the laboratories and accessory spaces for the Endoscopic Surgery Submodule


Endoscopic Surgery Submodule:PA = Reception, preparation of the animal (27 sqm) BPS = Room for biopsies ( 8 sqm) DS = Room for instruments disinfection, cleaning (17 sqm) SS = Sterilization room (17 sqm) V = Dressing room (17 sqm) CP = Lounge (35 sqm) WC = Sanitary closets, showers (17 sqm) NOTES ( 35 sqm) S = Surgical lavatory (17 sqm) CEX = Laboratory for experimental surgery (35 sqm) MAGAZIE - (cloak room – 17 sqm) HOL (hall – 88 sqm) TB. EL = Electric panel - (7 sqm)


II Pathology and Immunology Module

II.1 Pathology Submodule

II. PATHOLOGY AND IMMUNOLOGY MODULEDevice/Equipment/

System Specific requirements Utilities Location Area of research

II.1 PATHOLOGY SUBMODULEA. HISTOPATHOLOGY LABORATORY (HP)

1. Manual rotational microtome(Equipment for tissue and cells from fluids embedded in paraffin bloks sectionning)

• sectionning range 0,5-60 µm; • specimen retraction more than 100 µm - specimen orientation on 3 axis • trimming advance in 2 steps• standard specimen clamp and universal cassetes specimen holder

- power supply (220 V)- tap water- biological waste drain - air conditioning system

HPC p.1, C

p.2, C p.3, C p.4

2. Cryostat(Equipment for tissue sectionning at low temperature)

• cutting range: 0 - 60 µm• cooling down to –40ºC in steps of 1ºC• shock freezing system for cooling down to -60ºC• multiple cooling stations• automatic sterilyzing system, containing one formalin container, one waste container one pump and two injectors • specimen holder orientation on X,Y axis 15º min. and 360º on Z• vertical advance of the specimen holder min. 55 mm• horizontal advanceof the specimen holder min. 40 mm• Cutting with knifes and microtome blades • adjustable knife angle 0 – 30º• anti-roll system, operator protection against the knife sharp edge • 2-speed motorized coarse advance/retraction • microtome mechanism should allow specimen retraction at each stroke The microtome mechanism located on the oustside of the working chamber • fluorescent lamp illumination • window protection against condensate • cooling system located inside the cryostat base • automatic defrost at preset time or at user command


HP C p.1, C p.2, C p.3


B. IMMUNOHISTOCHEMISTRY AND IMMUNOCYTOCHEMISTRY LABORATORY

1. Immunohistocemistry automatic stainer (equipment for automatic staining of histo-pathology slides through the immunohistochemistry techniques)

Similar characteristics with those of the automatic stainer for standard staining


IHCICC C p.3, C p.6

C. IMAGE ANALYSIS LABORATORY

1. Microscopy system with halogen and UV illumination for the observation, aquisition, analysis of the histopathological images

a) Erect microscope for transmitted light brightfield observation and epi-fluorescence • infinity corrected universal plan apochromate objectives 2x, 4x/0.16, 10x/0.40, 20x/0.75, 40x/0,95 and 100x/1.40, • field of view min. 22 mm,• trinocular head with light path changer • swing-out front lens condenser with adjustable aperture diaphragm • built-in daylight and neutral density filters • halogen 100W transmitted light illumination and HBO 100W lamp for epi-fluorescence • epi-fluorescence condenser with motorized filter turret and UV protective shield • adjustable field and aperture diaphragms • filter sets for blue, green and UV excitations


ACI

C p.1, C p.2, C p.3, C p.4, C

p.5, C p.6, C p.9

b) Aquisition system • color digital photo/video camera with min. 5 Mpxl resolution• signal conversion board


ACI

c) Image analysis module:• Pentium 4 PC, updated configuration • image analysis and camera control software, including morphometry functions for automatic analysis


ACI

2. Laser microdissection system (Equipment for the precise selection of tissue and cell areas, through the Microarray and molecular pathology techniques)

a) Motorized inverted microscope • infinity corrected objectives with plan corection 4x, 10x, 20x and 40x, • motorized stage, • motorized focus • 10x eyepieces with wide field of view, min. 22 mm• epi-fluorescence accessories


ACI

C p.7, C p.8, C p.9

b) Dissection Module with UV-A 355 nm laser beam, picosecond impuls rate

- power supply (220 V)

ACI


• precise and fast cutting, repetition rate bigger than 5 KHz, • laser beam diameter <1µm, • laser beam < 1 µJoule for thermic tissue protection • contamination-free collecting system using „adhesive-cap” technology • motorized collector positionning/lifting/lowering controlled through microdissection software• adjustable cap pressure • possibility to isolate tissue areas with diameters up to 1 mm in one step• possibility to cut any tissue perimetral shape, regardless the staining type • special drawing pen to draw the cutting path on the touch-screen display (touch-screen monitor min. 20”, that allows full operation of the microdissection software),• ultra-high sensitivity , fire-wire technology, CCD min. 1/1.8”, super HAD technology, progressive scanning, resolution min. 3,2 Mpxl, binning function to increase the no. of fps, automatic and manual white balance, settings for fluorescence images, allow the control through the microdissection software,

- tap water- biological waste drain - air conditioning system

c) Digital control module • Pentium 4 PC updated configuration• system dedicated microdissection software


ACI

d) Antivibration table for dinamic vibrations absorbtion

- power supply (220 V)


Hereby, in cyan, on the plan, are depicted the locations for the laboratories and accessory spaces for the Pathology Submodule

Spaces in the Pathology Submodule include:

PMDF = Laboratory for development of documentation materials (area 35 sqm) TMED = Telepathology-Telemedicine Room (area 55 sqm) ACI = Image analysis laboratory (area 27 sqm) WC = Toilet (area 17 sqm) TB.EL = Electrical fuses room (area 7 sqm) DMR = Storage room for materials and reagents (area 21 sqm) CITHIS = Laser Microdisection, In situ Hybridization and Cytology Laboratory (area 47 sqm) HIS = In situ Hybridization (area 12 sqm) IHCICC = Immunohistochemistry and Immunocytochemistry Laboratory (area 10 sqm) HP = Histopathology Labotratory (area 35 sqm) CDDA = Rooms for Documentation and Diagnosis - RDD (area 17 sqm) CDDB = Rooms for Documentation and Diagnosis - RDD (area 17 sqm) SSLAN = Secretariat/LAN server (area 17 sqm)


II.2 Imunology Submodule

II. PATHOLOGY AND IMMUNOLOGY MODULEDevice/Equipment/System Specific requirements Utilities Location Area of

researchII.2 IMMUNOLOGY SUBMODULE

a) Flowcytometry Laboratory

1. Flow cytometry automated analyser

• 3 solid-state lasers linked to a fiber optic system, that analyses a minimum of 10 parameters• 10 detectors – 6 parameters/laser 1: light scattered at small angle (FSC – forward scatter), llight scattered at big angle (SSC – side scatter) and 4 fluorescent; 2 parameters/laser2; 2 parameters/laser 3, in total 8 detectors for fluorescent detection emission in different bands in the visible spectrum (blue, purple and red)• signal processing (up to 70000 events/s) and data aquisition are 100% digital• Inter- and intra-laser full-matrix compensation• automated Pre- or post-acquisition compensation• software with research applications as well as applications on cell cycle/DNA• software for automated analysis (aquisition / analysis) on 6 colours in the same sample/tube (fluorochromes)

- power supply (220 V)- continous water supply- evacuation of biological residues- air conditioning system

FLWC C i.1

b) ELISA Laboratory

1. Automated ELISA processor(Automated equipment for identification of molecular structures using immunoenzymatic reactions)

• Qualitative and quantitative analysis• Working capacity: 4 plates simoulaneously /12 parameters/plate • Up to 96 samples on line with continous-walk away framing for tubes 11-12.5 mm diameter, 55 –100 mm height;• Up to 50 positions for tubes 16 mm diameter, 100 mm height• Working with both primary and secondary tubes• CCD Bar Code Reader• Dedicated positions for reagents, standards and controls• Probe and reagent dilutions and predilutions• Sensors for detection of probe and reagent levels• Sensor for blood clots• Alarm for waste and washing solutions• CE MARK quality control software

- power supply (220 V)- continous water supply- evacuation of biological residues- air conditioning system

ELISA C i.2


Hereby, in blue on the plan, are depicted the locations for the laboratories and accessory spaces for the Imunology Submodule

Spaces in the Immunology Submodule include:

ELISA = Elisa Laboratory (area 35 sqm) DIP = Data Documentation, Interpretation and Processing (area 17 sqm) FLWC = Flowcytometry Laboratory (area 13 sqm) CPP = Sample Admission and Registration Room (area 13 sqm) WC = Toilet (area 35 sqm) TB.EL = Electrical fuses room (area 7 sqm)


III Molecular Biology and Biochemistry Module

III.1 Molecular Biology Submodule

III. MOLECULAR BIOLOGY AND BIOCHEMISTRY MODULE

Device/Equipment/System Specific requirements Utilities Location Area of research

III.1 MOLECULAR BIOLOGY SUBMODULEA. GENOMICS LABORATORY

1. Automated sample purification system coupled with Real-time PCR thermocycler (minimum 4 Emission filters, 2 detector chanells)

Automated pipetting system - Easy operation via control panel or PC - Preinstalled, validated applications and labware - High-precision pipetting from 1 µl to 1,000 µlReal Time PCR thermalcyclerThe fluorescent dyes chosen for each experiment are excited by an array of 96 individual LEDs. * The LEDs generate a blue light at a wavelength of ~470 nm, which can excite nearly all fluorophores used in realtime PCR (including SYBR®Green, FAM, VIC, TET, HEX, ROX, JOE, TAMRA). Emitted fluorescence is focused through an array of lenses and is passed to the optical detection unit through 96 individual optical fibers.


LGC g.1, C g.2, C g.3, C g.4

2. microarray analysis platform

This system includes:- GeneChip Scanner;- Fluidics Station;- Hybridization Oven;- a powerful computer workstation with dual Xeon processors loaded with GeneChip Operating Software.


LG C g.1, C g.2

3. Microfluidics-based platform for the analysis of DNA and RNA

Equipment Supplied with the Analyzer- Chip priming station;- IKA vortex mixer;- On chip analyzer;

- power supply (220V)- tap water- evacuation of biologic waste- air

LG C g.1, C g.2, C g.3, C g.4, C g.5




conditioning system

4. Class III biohazard safety cabinet. Vertical airflow

Biohazard safety cabinets offer the highest level of product, operator and environmental protection from infectious/biohazardous aerosols and are suitable for microbiological work with agents assigned to biological safety levels 1, 2, 3, or 4. Designed for an absolute level of containment, they are frequently used for work involving the deadliest biohazards, bacteria, viruses and microorganisms. Manufactured to meet and exceed the latest Class III biohazard safety cabinet requirements of the EN 12469:2000.- Exhaust air is double-filtered through high-quality ULPA filters (per IEST-RP-CC-001.3) with a rated efficiency of >99.999% for 0.1 to 0.3 micron particles, better than HEPA filters.- Exclusive dual exhaust filters provide >100,000 times better protection than single-stage designs.- Microprocessor-based Esco Sentinel™ Silver control system provides visual / audible alarms for airflow.- Magnehelic pressure gauge* is mounted in the rear of the work zone for at-a-glance monitoring of operating pressure.- Arm-length Neoprene™ gloves are chemical and flame resistant.- An integrated pass-through with interlocking doors permits materials transfer without risk of environmental contamination.- An angled cabinet front ensures an ergonomic working posture.- Cabinet operates at negative pressure relative to the laboratory in order to prevent migration of pathogenic materials out of the work area.


LGC g.1, C g.2, C g.3, C g.4, C g.5

5. Vacuum concentrator for DNA, RNA and proteins

- Chemical-resistant PTFE diaphragm pump;- Chemical-resistant stainless steel chamber;- Choice of three application modes (aqueous, alcohol or high vapour pressure) to correspond with sample solvent → reduction

- power supply (220V)- tap water- evacuation of biologic waste- air

LG C g.1, C g.2, C g.3,

C g.4, C g.5




of processing time up to 20%;- Choice of four heating levels (room temperature, 30 °C, 45° C, 60 °C) allows safe and efficient concentration of various samples;- Centrifugation and Desiccator function;- with built-in diaphragm pump and 48 x 1.5/2.0 ml fixed-angle rotor; -max. 144 tubes2 microplates

conditioning system

B. CYTOGENETICS LABORATORY

1. Complete system for karyotyping, FISH, MFISH, CGH and epi-fluorescence examination

- Motorized microscope, Sextuple Nosepiece, Trinocular Tube, Eyepiece, Abbe Condenser, 30W illuminator, 6-position fluorescence turret, fluorescence filter, fluorescence Lamp -130W, Optical fiber transmission , Plan Achromat X4, 20, 4- and Plan Fluor x10 and X100, digital cooled camera 2MPx, Digital Camera Control, C-mount, Adapter, UnitTV tube for C-mount adapter, software for automatic karyotyping, FISH, MFISH and CGH (comparative genomic Hybridization) , computer and LCD monitor


LCG C cg.1

2.Automatic Denaturation/Hybridization System

- 12 slide capacity for fast throughput - - Precise control of heating and cooling for more consistent FISH results-Programmable Temperature Controlled Slide -Automatic Denaturation/Hybridization Programable system


LCG C cg.2

3. Automatic Karyotyping System

-Microscope with trinocular tube, Plan Achromate 4x, 20x, 40x, 90x, 100x, LED illuminator, 2MPx digital camera, software for automatic karyotyping, computer


LCG C cg.3

4. VP 2000 Processor for slide Sistem procesare automata a lamelor

-Automatic pretreatment and staining, special stains (G-banding and other), and routine slide washing with a single system. The VP 2000 Processor -easily processes slides using

- power supply (220V)- tap water- evacuation of biologic

LCG C cg.1, C cg.2




pre-programmed protocols for fluorescence in situ hybridization (FISH) - solid tumor. Contain:PC computer, UPS, VP 200 software si VP 2000 procesor

waste- air conditioning system

5. Flow Hood class II-Samples protection, vertical and horizontal windows movement mechanism, safety system for air flow speed, air filter, air clean LCD display

- power supply (220V) LCG C cg.1, C

cg.2

6.Termostat Incubator/ThermostatCell culture

-Temperature range of 7 °C (13 °F) above abient temperature up to 60 °C (140 °F), Temperature variation at 37 °C : ± 0.3, interface for communication , Electronic self-diagnostic system for errors with optical and acoustic alarm, Perforated shelves, stainless steel, controller for temperature and CO2 concentration.


LCG C cg.1, C cg.2

7.Centrifuge-Speed: 5.000 rpm, Programmable RPM and RCF , Digital display:time and RPM


LCG C cg.2

8. Heating Magnetic stirrer

-Speed range 0 - 1500 1it/min , Heating rate 7°C /min , Heating temperature range room temp. - 340°C, Heat control accuracy 10 ±°C, Connection for ext. temperature sensor ETS-D 4 fuzzy, Control accuracy with sensor 1± °C


LCG C cg.1, C cg.2

9. Thermostatic water multiple baths-

-Independent temperature regulation of every bath. Tank for every bath, Bath :4, Tempt range:Amb.+120, Precision at base °C:± 1,5; Heating power W:1600

- power supply (220V)- tap water- air conditioning system

LCG C cg.1, C cg.2


C. CELL CULTURES LABORATORY

1. Incubator with CO2

Lampa UV lamp; Perforated shelves, stainless steel, air flow system computer assited, sensor CO2;


LCC C c.1, C c.2, C c.3, C c.4

2. Flow Hood class IISamples protection, vertical and horizontal windows movement mechanism, safety system for air flow speed, air filter, air clean LCD display


LCC C c.1, C c.2, C c.3, C c.4

3. Refrigerated centrifuge with interchangeable-rotor

Microprocessor and digital display, induction motor, Programmable RPM and RCF Increments of 10; stainless steel, 9 level of preselection, protection of centrifuge motor, Automatic rotor recognition,


LCC C c.1, C c.2, C c.3, C c.4

4.Laboratory microcentrifuge

Microprocessor and digital display, automatic sensor blocks centrifuge door, stainless steel, Programmable RPM and RCF Increments of 10, max. speed/RCF: 1800 rpm/23.907; volume 6*50 ml; timer 1....99 min


LCC C c.3, C c.4

5. Inverted fluorecence microscope for cell cultures examination with PC camera

For examination cell cultures and the changes appeared in flasks (numer and morphology of cells), digital camera connected at PC for analysis


LCC C c.1, C c.2, C c.3, C c.4

6. AnalyticalBalance

internal calibration , adjustable and time control, detrmination of the fluids density

- power supply (220V)

LCC C c.1, C c.2, C c.3, C c.4

Hereby, in green on the plan, are depicted the locations for the laboratories and accessory spaces for the Molecular Biology Submodule


Spaces in the Molecular Biology Submodule include:

SS = sterilization area (15 sqm) SR = sample collecting area (12 sqm) SV = cloakroom (12 sqm) SC = sample storage area (16 sqm) SCAD = Secretariat, documentation and analysis area (24 sqm) LCG = Cytogenetics area (68 sqm) LCG1 = sample processing area (16 sqm) LCG2 = standard karyotyping area (17 sqm) LCG3 = FISH analysis area (12 sqm) LCG4 = CGH analysis area (12 sqm) Lobby = (11 sqm) LCC = Cell cultures laboratory (52 sqm) LCC1 = sample processing area (17 sqm) LCC2 = incubating area (12 sqm) LCC3 = microscopy area (12 sqm) Lobby = (11 sqm) LG = Genomics laboratory (79 sqm) LG1D = DNA Pre-amplification area (6 sqm) LG2D = DNA amplification area (8 sqm) LG3 = post PCR area (28 sqm) LG1R = RNA Pre-amplification area (6 sqm) LG2R = DNA amplification area (9 sqm) CR = Reagents area (5 sqm) Lobby = (17 sqm)


III.2 Biochemistry Submodule



III.2 BIOCHEMISTRY SUBMODULE

1. 2D electrophoresis system

Isoelectric focusing system with: power source (10000 V, 2.4 mA); platform for up to 24 strips of 7, 12 strips of 11, 17, 24 cm with a precise temperature control at 10-25oC (Peltier system); rehydration and equilibration trays for 12 strips; isoeletric focusing tank including platinium electrodes assuring sample integrity; collecting data via RS-232 serial port; interface for method programmation and providing continuous information on the migration process; IPG strips with different pH gradient (3–6, 5–8, 7–10), labeled for rapid identification of polarity and pH rangecompact vertical electrophoresis system for 12 gels simultaneous processing; cooling system, buffer recycling vacuum; plate electrodes making an equal electric field; versatility for different gel sizes (18,3x19,3 cm, 18,5x20 cm, 20x20,5 cm, 25x20,5 cm) and different thickness (1-3 mm); spacers; accessory for stabilizing loaded gels;appropriate power source (250 V, 3 A, 300 W)scanner with high resolution for quantitative evaluation of spots and appropriate software for protein analysis

- energy supply (220 V)- tap water- evacuation of biological waste - air conditioning system

MLDC p.1, C

p.2, C p.3, C p.4

2. MALDI -TOF-MS system

microScout Ion Source with state-of-the-art pulsed ion extraction (PIE™); target area exactly ¼ of industry standard microtiter plates; various 96-spot target types available for specific applications; nitrogen laser with variable repetition rate up to 20 Hz; TOF analyzer: high resolution reflectron configuration or optional linear only version; optional MS/MS capability with auto PSD (automated post-source decay) including precursor ion selection device; modular design allows for future upgrades; WhisperMode™ erases noise pollution; software for data analysis

- energy supply (220 V)- tap water - evacuation of biological waste- air conditioning system

MLDC p.1, C

p.2, C p.3, C p.4

3.Homogenising system Sonication system; homogenising system with liquid nitrogen

- energy supply (220 V)

MLD C p.1, C p.2, C p.3,




- tap water- evacuation of biological waste- air conditioning system

C p.4

4. Luminiscence spectrophotometer

Analysis of luminescence, excitation, phosphorescence spectra in (200-800nm) range, photomultiplier detection, automatic correction of spectra, polarized light measurements, bio-luminescence measurements, sample temperature control, variable gate and delay of excitation, flow-cytometry option, plate reader option for chemiluminescence, CCD detection, software for data analysis


RL C p.5

5. Chemiluminiscence detection system for Western blots

Dual system of excitation and emission for protein detection on Western blots; emission wavelenght:488 si 560 nm; 2D imaging; CCD detection; detector temperature control; UV filter; real time working; transiluminator, EPI-iluminator; software for image analysis and processing


MCFC p.1, C

p.2, C p.3, C p.4

6. Atomic Force Microscope AFM

Atomic force microscope with operating modes: contact and true non-contact Atomic Force Microscopy, Lateral Force Mode, AFM-SPM mode for operation in liquid environment, intermittent contact mode, phase imaging force modulation mode, Force Mode, nanolithography, EFM – Electrostatic Force Microscopy, Adhesion Force Imaging, software for 3D analyses, inverted optical, microscope, XY scan > 90 m, active and passive devices for damping of vibrations, computer, noise Z<0.03nm-RMS, 1000Hz, noise XY<0.01nm-RMS 200Hz, optical inverted microscope


AFM C p.6

7. Refrigerator - 80°C 2 doors, no-frost, anti-bacteria, temp -20oC +4°C


SDC p.1, C

p.2, C p.3, C p.4, C p.5

8. Precision balance

0-300g, digital display with liquid cristals, internal calibration, RS 232 C interface; glass weighing space, gliding lateral doors, readability 0.1 mg


LPPC p.1, C

p.2, C p.3, C p.4, C p.5




9. AutoclaverFor humide sterilization, capacity 40L; working temperature: 120°C; working pressure: 1,2-2 atm


CADC p.1, C

p.2, C p.3, C p.4, C p.5

10. Thermostated waterbath

Working domain: 30-100°C; capacity: 20L; accuracy of temperature control using external sensor 1°C


CADC p.1, C

p.2, C p.3, C p.4, C p.5

11. Drying ovenFor dry sterilization; capacity 120L; temperature range 40-300°C; programmable working time

- energy supply (220 V)- evacuation of biological wastes - air conditioning system

CADC p.1, C

p.2, C p.3, C p.4, C p.5

12. Two-photon laser scanning microscope

For longitudinal, simultaneous analysis of the dynamic of multiple genetic and protein markers; allows small sample analysis without affecting adjacent regions; excitation with saphira laser; spectral domain: 720-920 nm; detection system: multi-fotomultiplicator; system for X-Y with tridimensional reconstruction of cell structures; software for deconvolution

- energy supply (220 V)- tap water- evacuation of biological waste - air conditioning system-vibration-free platform

MCF C p.6

Hereby, in green on the plan, are depicted the locations for the laboratories and accessory spaces for the Biochemistry Submodule


Spaces in the Biochemistry Submodule include:

MCF= Laboratory for confocal two photons microscopy (area 51 sqm) SS = Server Room (area 17 sqm) CAD = Center for Data Analysis (area 30 sqm) MLD = Laboratory for MALDI-TOF analysis (area 50 sqm) SD = Storage space (area 25 sqm) LPP = Laboratories for samples processing (area 65 sqm) Halls (area 53 sqm)AFM = Laboratory for atomic force microscopy (area 35 mp) LL = Laboratory for luminiscence measurement (suprafata 50 mp)


Software and hardware acquisition

Next table presents the necessary IT equipments for TARGET modules. First column presents the equipment type, the second presents the minimal technical requirements and the third the connection with the types of research which utilizes the equipments.

NOTE!!! For types of research visualization please see 2.b.1 “scientific justification of investment”.

I. DIGESTIVE IMAGING MODULE

I. DIGESTIVE IMAGING MODULEEquipment/systems Minimal technical specifications Research implication

1. PC Server x 1P4 quad-core 2.6 GHz, 4Gb RAM, 2xHDD 500 Gb, DVD Writer, video acquisition interface, preinstalled OS Windows

C t.1, C t.2, C t.3, C v.1

2. PC workstations x 2P4 quad-core 2.6 GHz, 2Gb RAM, 2xHDD 250 Gb, DVD Writer, preinstalled OS Windows

C t.1, C t.2, C v.1

3. Monitor x 3 LCD 20” C t.1, C t.2, C v.14. UPS x 2 2500 VA C t.1, C t.2, C v.15. Printer x 2 laser color A4 600dpi C t.2, C t.3, C v.16. Multifunctional printer x 1 laser A4, printer/scanner/copier/fax C t.2, C t.3, C v.17. Scanner x 1 high resolution A3 2400 dpi 24bit C t.18. Switch/router x 1 1000Mbps layer 3 with management C t.1, C t.3

9. Wireless gateway x 1 4 UTP 100Mbps, wireless, USB hub, HDD 500Gb, print server, port SATA C t.1, C t.3

10. Video-conference equipment x 2

Terminal, onboard camera with zoom, connectivity LAN/ISDN, multiple video inputs, dual-stream

C t.1, C t.3

11. Monitor/tuner x 2LCD 37”, 16:9 PIP/POP, RGB/Composite/S-video/DVI inputs, wall mounted

C t.1, C t.3

12. Audio/video switch x 1 composite; video; RGB C t.1, C t.313. DVD x 2 PAL VHS/HDD/DVD/card recorder C t.1, C t.3

14. Notebook x 2

Processor Core 2 Duo 1.8GHz, 2GB, 250GB SATA, 128MB VRAM, monitor WXGA TFT, DVD-RW/CD-RW (Multiburner), Modem, Wireless 11a/b/g, Bluetooth, network 10/100/1000, Camera, Microphone, 3xUSB, preinstalled OS Windows

C t.1, C t.2, C v.1

15. PDA x 2

400MHz, 64Mb, IrDA, USB, serial, Mini SD, display 3” 64k color, camera 1.3Mpix, GSM/GPRS/EDGE, Bluetooth, wireless, preinstalled OS Windows Mobile

C t.1, C t.2, C v.1

16. MS Office x 9 Microsoft office applications C t.2, C v.117. Corel Draw x 1 Object oriented editing C t.2, C v.118. Adobe Photoshop x 1 Graphic image editing C t.2, C v.119. Adobe Premiere x 1 Video image editing C t.2, C v.120. Antivirus x 9 Virus and firewall protection PC C t.2, C v.121. Windows x 9 Operating system


II. PATHOLOGY AND IMMUNOLOGY MODULE

II. PATHOLOGY AND IMMUNOLOGY MODULEEquipment/systems Minimal technical specifications Research implication


C t.1, C t.2, C t.3, C v.1


C t.1, C t.2, C v.1





C t.1, C t.3

11. Monitor/tunerLCD 37”, 16:9 PIP/POP, RGB/Composite/S-video/DVI inputs, wall mounted

C t.1, C t.3

12. Audio/video switch x 1 composite; video; RGB C t.1, C t.3

13. Notebook x 2


C t.1, C t.2, C v.1

14. PDA x 2400MHz, 64Mb, IrDA, USB, serial, Mini SD, display 3” 64k color, camera 1.3Mpix, GSM/GPRS/EDGE, Bluetooth, wireless, preinstalled OS Windows Mobile

C t.1, C t.2, C v.1

15. MS Office x 9 Microsoft office applications C t.2, C v.116. Corel Draw x 1 Object oriented editing C t.2, C v.117. Adobe Photoshop x 1 Graphic image editing C t.2, C v.118. Adobe Premiere x 1 Video image editing C t.2, C v.119. Antivirus x 9 Virus and firewall protection PC C t.2, C v.120. Windows x 9 Operating system C t.2, C v.1


III. MOLECULAR BIOLOGY AND BIOCHEMISTRY MODULEEquipment/systems Minimal technical specifications Research implication


C t.1, C t.2, C t.3, C v.1


C t.1, C t.2, C v.1



III. MOLECULAR BIOLOGY AND BIOCHEMISTRY MODULEEquipment/systems Minimal technical specifications Research implication




C t.1, C t.3

11. Monitor/tuner x 1LCD 37”, 16:9 PIP/POP, RGB/Composite/S-video/DVI inputs, wall mounted

C t.1, C t.3

12. Audio/video switch x 1 composite; video; RGB C t.1, C t.3

13. Notebook x 2


C t.1, C t.2, C v.1

14. PDA x 2400MHz, 64Mb, IrDA, USB, serial, Mini SD, display 3” 64k color, camera 1.3Mpix, GSM/GPRS/EDGE, Bluetooth, wireless, preinstalled OS Windows Mobile

C t.1, C t.2, C v.1

15. MS Office x 9 Microsoft office applications C t.2, C v.116. Corel Draw x 1 Object oriented editing C t.2, C v.117. Adobe Photoshop x 1 Graphic image editing C t.2, C v.118. Adobe Premiere x 1 Video image editing C t.2, C v.119. Antivirus x 9 Virus and firewall protection PC C t.2, C v.120. Windows x 9 Operating system C t.2, C v.1

III. TELEMEDICINE AND ARTIFICIAL INTELLIGENCE MODULE

4.1 Telecommunication and Database Submodule IV. TELEMEDICINE AND ARTIFICIAL INTELLIGENCE MODULE

Equipment/systems Minimal technical specifications Utilities required Location Research

implication4.1.Telecommunication and Database Submodule

1. DB server x 2Data storage server; dual configuration with mass-storage battery 2xP4 3GHz, 8Gb RAM, 4x500Gb HDD, DVD-writer

Power supply (220 V)

SGBD C t.1, C t.2, C t.3, C v.1, C v.4

2. Server web/ftp/mail/internet services x 1

2xP4 3GHz, 8Gb RAM, 4x500Gb HDD, DVD-writer, real-time video streaming interface

Power supply (220 V) TCOM C t.1, C t.3

3. Router/gateway x 1

2xP4 3GHz, 8Gb RAM, 4x500Gb HDD, DVD-writer, real-time video streaming interface

Power supply (220 V) TCOM C t.1, C t.3

4. Monitor x 4 LCD 22” Power supply (220 V)

SGBD, TCOM

C t.1, C t.2, C v.1

5. UPS x 2 3000 VA Power supply (220 V)

SGBD, TCOM

C t.1, C t.2, C v.1

6. Printer x 1 laser color A4 600dpi Power supply (220 V) SGBD C t.2, C t.3, C

v.17. Multifunctional printer x 1

laser A3, printer/scanner/copier/ fax

Power supply (220 V) SGBD C t.2, C t.3, C

v.1

8. Integrated design system x 1 Printer/copier/scanner A0 color Power supply

(220 V) SGBD

C t.1, C t.2, C t.3, C v.1, C v.2, C v.3, C

v.4


IV. TELEMEDICINE AND ARTIFICIAL INTELLIGENCE MODULEEquipment/systems Minimal technical specifications Utilities

required Location Research implication

9. Switch/router x 1 1000Mbps layer 3 with management

Power supply (220 V) SGBD C t.1, C t.3

10. Wireless gateway x 1

4 UTP 100Mbps, wireless, USB hub, HDD 500Gb, print server, port SATA

Power supply (220 V) SC C t.1, C t.3

11. Router x 1 professional backbone router Power supply (220 V) TCOM C t.1, C t.3




13. Monitor/tuner x 1

LCD 37”, 16:9 PIP/POP, RGB/Composite/S-video/DVI inputs, wall mounted


14. Audio/video switch x 1 composite; video; RGB Power supply

(220 V) SC C t.1, C t.3

15. Video-projector x 1

XGA/WXGA minim 1024x768 minim 3000 hours, distance 12m


16. Video camera x 2

connectivity IP, motion detector, color night view, wall mounted


SGBD, TCOM C t.1, C t.3

17. DVD x 1 PAL VHS/HDD/DVD/card recorder


18. Notebook x 1



v.1

19. Rack x 1 42U, with cooling system Power supply (220 V) TCOM C t.1, C t.2, C

v.1

20. PDA x 1

400MHz, 64Mb, IrDA, USB, serial, Mini SD, display 3” 64k color, camera 1.3MPix, GSM/GPRS/EDGE, Bluetooth, wireless, preinstalled OS Windows Mobile


v.1

21. Access point x 2 wireless IEEE 802.11g 56Mbps omnidirectional 500m

Power supply (220 V) SC, TCOM C t.1, C t.2, C

v.1

22. Infrastructurecable organizer, UTP cable, UTP sockets, UTP connectors, crimping tool, UTP cable tester, media convertor

Power supply (220 V) TCOM C t.1

23. UPS x 1 40kVA/32kW, three-phase / 25 min

Power supply (220 V) UPS

C t.1, C t.2, C v.1, , C v.2, , C

v.3, , C v.4

24. UPS x 1 30kVA/24kW, three-phase/ 25 min

Power supply (220 V) UPS

C t.1, C t.2, C v.1, , C v.2, , C

v.3, , C v.4

25. PBX x 1IP phone PBX, 4 analog trunks, 2 ISDN, min 100 IP phones, min 100 analog ext, optional T1/E1

Power supply (220 V) TCOM

26. MS Office x 5 Microsoft office applications Power supply (220 V) SGBD C t.2, C v.1

27. Corel Draw x 1 Object oriented editing Power supply (220 V) SGBD C t.2, C v.1

28. Adobe Photoshop x 1 Graphic image editing Power supply

(220 V) SGBD C t.2, C v.1




29. Adobe Premiere x 1

Video image editing Power supply (220 V)

SGBD C t.2, C v.1

30. Antivirus for server x 4

Virus and firewall protection server


SGBD C t.2, C v.1

31. Windows 2003 server x 4

Server operating system Power supply (220 V)

SGBD C t.2, C v.1

32. SQL server x 4 Database server system Power supply (220 V)

SGBD C t.2, C v.1

Hereby, in yellow on the plan, are depicted the locations for the laboratories and accessory spaces for the Telecommunications and Database Submodule


The locations of the Telecommunications and Database Submodule are:

TCOM = Communication Management Center (surface 35 sqm) UPS = Uninterruptable Power Supply (surface 39 sqm) DEPOZIT = Storage Room (surface 6 sqm) HOL = Corridor (surface 5 sqm)SGBD = Database Management Center (surface 174 sqm) SC = Conference Management Center (surface 36 sqm) ARHIVA = Multimedia Archive (surface 36 sqm)

4.2 Artificial Intelligence and Statistic Processing Submodule



4.2 ARTIFICIAL INTELLIGENCE AND STATISTIC PROCESSING SUBMODULE


Power supply (220 V) IA/VR

C t.2, C t.3, C v.1, C v.2, C

v.3, C v.4

2. PC workstations x 6

P4 quad-core 2.6 GHz, 2Gb RAM, 2xHDD 250 Gb, DVD Writer, preinstalled OS Windows


C t.2, C v.1, C v.2, C v.3, C

v.4

3. Monitor x 7 LCD 20” Power supply (220 V) IA/VR

C t.2, C v.1, C v.2, C v.3, C

v.4

4. UPS x 2 2500 VA Power supply (220 V) IA/VR

C t.2, C v.1, C v.2, C v.3, C

v.4

5. Printer x 1 laser color A4 600dpi Power supply (220 V) IA/VR

C t.2, C t.3, C v.1, C v.2, C

v.3, C v.4

6. Multifunctional printer x 1

laser A4, printer/scanner/copier/fax


C t.2, C t.3, C v.1, C v.2, C

v.3, C v.4

7. Scanner 3D x 1 multilaser color 3D scanner, 200dpi, field size 13.5”x10.1”


C t.1, C t.2, C v.1, C v.2, C

v.3, C v.4

8. Wireless gateway x 1

4 UTP 100Mbps, wireless, USB hub, HDD 500Gb, print server, port SATA


C t.2, C t.3, C v.1, C v.2, C

v.3

9. Notebook x 2



C t.2, C v.1, C v.2, C v.3, C

v.4

10. PDA x 2

400MHz, 64Mb, IrDA, USB, serial, Mini SD, display 3” 64k color, camera 1.3Mpix, GSM/GPRS/EDGE, Bluetooth, wireless, preinstalled OS Windows Mobile

Power supply (220 V) IA/VR C t.2, C v.1, C

v.2, C v.3

11. Drivers + SDK kit x 1

Drivers for Windows XP and software development SDK

Power supply (220 V) IA/VR C v.4




12. MS Office x 9 Microsoft office applications Power supply (220 V) IA/VR C t.2, C v.1, C

v.2, C v.3

13. Corel Draw x 1 Object oriented editing Power supply (220 V) IA/VR C t.2, C v.1, C

v.2, C v.314. Adobe Photoshop x 1 Graphic image editing Power supply

(220 V) IA/VR C t.2, C v.1, C v.2, C v.3

15. Adobe Premiere x 1 Video image editing Power supply

(220 V) IA/VR C t.2, C v.1, C v.2, C v.3

16. Antivirus x 9 Virus and firewall protection PC Power supply (220 V) IA/VR C t.2, C v.1, C

v.2, C v.317. Microsoft C++ x 1

Programming Language Environment


v.2, C v.3

18. SPSS x 1 Statistic analysis application tools Power supply (220 V) IA/VR C t.2, C v.1, C

v.2, C v.319. Statistic Application x 1 Data analysis application tools Power supply

(220 V) IA/VR C t.2, C v.1, C v.2, C v.3

20. Visual Studio x 1

mission-critical, multi-tier, smart client, Web, mobile and Microsoft Office based application


v.2, C v.3

The locations of the Artificial Intelligence and Statistic Processing Submodule are:

IA/VR = Artificial Intelligence / virtual reality laboratory (surface 35 sqm)


Furniture acquisitionIt is needed specific furniture acquisition for TARGET modules. Below there are the tables with special furniture for each module and their characteristics.

The following table presents the minimal characteristics that the special furniture must have for endowment of the laboratories of the Radiology and Imaging Submodule

Necessary furniture Characteristics: D=depth, W=width, H=heightI Furniture Digestive Imaging Module

I.1 Radiology and Imaging Submodule (NEW BUILDING)UNDERGROUND

Support roomDesk computer with drawer pedestal with 3

drawers (2 pieces) Simple desk H 75 cm / W 160 cm / D 80 cm

Desk chair (2 pieces) Desk chairLucker cabinet with doors Cabinet H 200 cm / W 105 cm / D 35 cm

Bookcases with doors and shelves (2 pieces) Bookcase H 185 cm / W 75 cm / D 35 cmOil deposit

Metal cabinets with shelves (2 pieces) H 220 cm/ W 75 cm / D 35 cmGROUND FLOOR

Support roomDesk with drawer pedestal with 3 drawers (2

pieces) Simple desk H 75 cm / W 160 cm / D 80 cm

Desk chair (2 pieces) Desk chairLucker cabinet with doors Cabinet H 200 cm / W 105 cm / D = 35 cm

Bookcases with doors and shelves (2 pieces) Bookcase H 185 cm / W 75 cm / D 35 cmPatient waiting room

Chairs (40 pieces) Plastic chairsPatient registration

Computer desk with drawer pedestal with 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cm

Desk with drawer pedestal with 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cmMobile, rotation chair with back (2 pieces) Elbow supportBookcases with doors and shelves, with

transparent glass doors (2 pieces) Bookcase H 185 cm / W 75 cm / D 35 cm

Ergonomic chair (2 pieces) Plastic chairs with textile supportActive patient waiting room

Chairs (10 pieces) Plastic chairsPassive patient waiting room

Chairs (10 pieces) Plastic chairsRoom for personnel


Desk with drawer pedestal with 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cmDesk chair (2 pieces) Leather desk chair

Locker cabinet with doors Cabinet H 200 cm / W 105 cm / D = 35 cmBookcases with doors and shelves, with

transparent glass doors Bookcase H 185 cm / W 75 cm / D 35 cm

Ergonomic chair (2 pieces) Plastic chairs with textile supportLabor

Desk with drawer pedestal with 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cmErgonomic chair (2 pieces) Plastic chairs with textile support

Bookcases with doors and shelves, with transparent glass doors Bookcase H 185 cm / W 75 cm / D 35 cm

PET results analysisComputer desk with drawer pedestal with 3

drawers Simple desk H 75 cm / W 160 cm / D 80 cm

Desk with drawer pedestal with 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cm



Desk chair (2 pieces) Leather desk chairBookcases with doors and shelves, with


Isotope depositMetal cabinets with shelves (4 pieces) H 220 cm/ W 75 cm / D 35 cm

Support roomBookcases with doors and shelves (2 pieces) Bookcase H 185 cm / W 75 cm / D 35 cm

PET command roomComputer desk with drawer pedestal with 3


Mobile, rotation chair with back (4 pieces) Leather, with elbow supportPreparation

Computer desk with drawer pedestal with 3 drawers (2 pieces) Simple desk H 75 cm / W 160 cm / D 80 cm

Mobile, rotation chair with back (2 pieces) Leather, with elbow supportBookcases with doors and shelves, with


FIRST FLOORSupport room

Desk with drawer pedestal with 3 drawers (2 pieces) Simple desk H 75 cm / W 160 cm / D 80 cm

Desk chair (2 pieces) Desk chairLocker cabinets with doors Cabinet H 200 cm / W 105 cm / D = 35 cm

Bookcases with doors and shelves (2 pieces) Bookcase H 185 cm / W 75 cm / D 35 cmPhysicist desk



Bookcases with doors and shelves, with transparent glass doors (2 pieces) Bookcase H 185 cm / W 75 cm / D 35 cm

Technician deskComputer desk with drawer pedestal with 3




Delivery deposit

Modular cabinets with doors (6 pieces) Cabinet with doors in the superior part and shelves in the inferior part: H 220 cm/ W 75 cm / D 35 cm

Quality controlComputer desk with drawer pedestal with 3



Support, technical service, gas deposit


Desk with drawer pedestal with 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cmDesk chair Leather desk chair

Cyclotron command roomComputer desk with drawer pedestal with 3


Mobile, rotation chair with back (4 pieces) Leather, with elbow supportOutput, C class lab




Desk chair (2 pieces) Leather desk chairMobile, rotation chair with back (2 pieces) Leather, with elbow supportBookcases with doors and shelves, with


Output, B class labDesk with drawer pedestal with 3 drawers (2

pieces) Simple desk H 75 cm / W 160 cm / D 80 cm

Desk chair (2 pieces) Leather desk chairMobile, rotation chair with back (2 pieces) Leather, with elbow supportBookcases with doors and shelves, with


SECOND FLOORSupport room


Desk chair (2 pieces) Desk chairLocker cabinet with doors Cabinet H 200 cm / W 105 cm / D = 35 cm

Bookcases with doors and shelves (2 pieces) Bookcase H 185 cm / W 75 cm / D 35 cmRoom for personnel





Ergonomic chair (2 pieces) Plastic chairs with textile supportPatient registration

Computer Desk with drawer pedestal with 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cm

Desk with drawer pedestal with 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cmMobile, rotation chair with back (2 pieces) Elbow supportBookcases with doors and shelves, with


Ergonomic chair (2 pieces) Plastic chairs with textile supportPatient waiting room

Chairs (40 pieces) Plastic chairsRoom for personnel





Ergonomic chair (2 pieces) Plastic chairs with textile supportPatient waiting room

Chairs (30 pieces) Plastic chairsCommand room


Mobile, rotation chair with back (4 pieces) Leather, with elbow supportTHIRD FLOORSupport room


Desk chair (2 pieces) Desk chairLocker cabinet with doors Cabinet H 200 cm / W 105 cm / D = 35 cm



Bookcases with doors and shelves (2 pieces) Bookcase H 185 cm / W 75 cm / D 35 cmWomen dressing room

Locker cabinet with doors (2 pieces) Cabinet H 200 cm / W 105 cm / D = 35 cmMen dressing rom

Locker cabinet with doors (2 pieces) Cabinet H 200 cm / W 105 cm / D = 35 cmKitchen

Table (2 pieces) H 75 cm / W 160 cm / D 80 cmChairs (8 pieces) Chairs

Cabinet with doors and shelves H 185 cm / W 75 cm / D 35 cmLibrary




Desk chair (4 pieces) Leather desk chairErgonomic chair (4 pieces) Plastic chairs with textile support

Room for personnel 1Computer Desk with drawer pedestal with 3



Desk chair (2 pieces) Leather desk chairLocker cabinet with doors (2 pieces) Cabinet H 200 cm / W 105 cm / D = 35 cm


Ergonomic chair (2 pieces) Plastic chairs with textile supportRoom for personnel 2



Desk chair (2 pieces) Leather desk chairLocker cabinet with doors (2 pieces) Cabinet H 200 cm / W 105 cm / D = 35 cm


Ergonomic chair (2 pieces) Plastic chairs with textile supportPhysicist desk




Physicist desk-LTGComputer Desk with drawer pedestal with 3




Archive


ServerCabinets with sliding doors from temperate

glass and 4 shelves H 200 cm/W 120 cm/D 45 cm



Modular cabinet with sliding doors from temperate glass and 1 shelf H 74 cm/W 120 cm/D 45 cm

Vertical rack with ventilation Rack 19” 42UWork desk H 75 cm/W 150 cm/D 75 cm

The following table presents the minimal characteristics that the special furniture must have for endowment of the laboratories of the Digestive Endoscopy Submodule

I Furniture Digestive Imaging ModuleI.2 Digestive Endoscopy Submodule

Necessary furniture Characteristics: D=depth, W=width, H=heightAutofluorescence Endoscopy Laboratory (AFI)

Sofa for endoscopic examination Mobile sofa on horizontal or vertical, with electrical drive, with rack for perfusions


Desk chair Leather desk chairModules for emergency medication with doors

from transparent glassCabinet with sliding doors from temperate glass: H 185

cm/ W 75 cm/ D 35 cmExamination chairs for doctors and nurses (3

pieces) Rotating chairs with adjustable height, H 60 cm

Modular cabinets with doors Cabinet with doors in the superior part and shelves in the inferior part H 220 cm/ W 75 cm / D 35 cm

Bookcases with doors and shelves, with transparent glass doors Book case H 185 cm / W 75 cm / D 35 cm

Locker cabinet Cabinet H 200 cm / W 105 cm / D = 35 cm

Endoscopy cabinetCabinet with metallic structure H 200 cm / W 105 cm /

D = 35 cm, with special ventilation, support for 4 endoscopes

Fixing system endoscopy tower Fixing system for endoscopes, ceiling mounting system

Venetian blinds (2 pieces) 1.5x2.5Magnification Chromoendoscopy Laboratory (MCE)

Sofa for endoscopic examination Mobile sofa on horizontal or vertical, with electrical drive, with rack for perfusions


Desk chair Leather desk chairModules for emergency medication with doors

from transparent glassCabinet with sliding doors from temperate glass: H

185 cm/ W 75 cm/ D 35 cmExamination chairs for doctors and nurses (3

pieces) Rotating chairs with adjustable height, H 60 cm

Modular cabinets with doors Cabinet with doors in the superior part and shelves in the inferior part: H 220 cm/ W 75 cm / D 35 cm

Bookcases with doors and shelves, with transparent glass doors Book case H 185 cm / W 75 cm / D 35 cm

Locker cabinet Cabinet H 200 cm / W 105 cm / D = 35 cm

Endoscopy cabinet Cabinet H 200 cm / W 105 cm / D = 35 cm, with special ventilation, support for 4 endoscopes


Venetian blinds (2 pieces) 1.5x2.5Monitoring hall, post procedural Endoscopic Autofluorescence and Magnification

Chromoendoscopy (MON AFI MCE)Sofas for patients in monitoring room

(3 pieces) Mobile beds foreseen with lateral protector and rack



Necessary furniture Characteristics: D=depth, W=width, H=heightArmchairs for waiting room Leather simple arm chairs

Sofas for waiting room Leather sofa with 3 placesChairs (2 pieces) Plastic chair with textile support

PVC door (2 pieces) H 200 cm, W150Venetian blinds (2 pieces) 1.5x2.5

Anesthesia Modules Anesthesia apparatus+ monitor vital functions + cylinders O2

Disinfection hall (SS)Venetian blinds 1.5x2.5

Washing machines for endoscopes(2 pieces) 1.5x0.4 (automatic machine, with 2 endoscopes)

System for disinfection of endoscopes+ accessories

Ultrasonic cleaning system, controlled through microprocessor (cleaning cycle 1-30 minutes)

Ventilation installation Air ventilation 20 mc/minUltrasound Endoscopy Laboratory (EUS)

Sofa for endoscopic examination Mobile sofa on horizontal or vertical, with electrical auctioning, with rack for perfusions

Modules for emergency medication with doors from transparent glass.

Cabinet with sliding doors from temperate glass: H 185 cm/ W 75 cm/ D 35 cm

Examination chairs for doctors and nurses (3 pieces) Rotating chairs with adjustable height, H 60 cm

Cabinet mask sink Cabinet with 4 doors H 70 cm / W 160 cm / D 35 cm, with depositing shelf





Venetian blinds (2 pieces) 1.5x2.5Confocal Laser Endomicroscopy Laboratory (CLE)

Sofa for endoscopic examination Mobile sofa on horizontal or vertical, with electrical auctioning, with rack for perfusions

Modules for emergency medication with doors from transparent glass.


Examination chairs for doctors and nurses (3 pieces) Rotating chairs with adjustable height , H 60 cm

Cabinet mask sink Cabinet with 4 doors H 70 cm / W 160 cm / D 35 cm, with depositing shelf





Venetian blinds (2 pieces) 1.5x2.5Hall of post procedure monitoring Ultrasound Endoscopy and Confocal Laser Endomicroscopy

(MON EUS CLE)Sofas for patients in monitoring room (2

pieces) Mobile beds foreseen with lateral defenders and rack

Armchairs for waiting room Leather simple arm chairsSofas for waiting room Leather sofa with 3 places

Simple chairs (2 pieces) Plastic chair with textile supportPVC door (2 pieces) H 200 cm, W150

Venetian blinds (2 pieces) 1.5x2.5Studiu de fezabilitate – TARGET SF 88


Necessary furniture Characteristics: D=depth, W=width, H=height

Anesthesia Modules Anesthesia apparatus+ monitor vital functions + cylinders O2

SecretariatComputer desk with drawer pedestal with 3


Desk chair Leather desk chairBookcases with doors and shelves, with

transparent glass doors Book case H 185 cm / W 75 cm / D 35 cm

Locker cabinet with doors Cabinet H 200 cm / W 105 cm / D = 35 cmSofa for office Leather sofa with 3 places

Table Diverse tablesPVC door (2 pieces) H 200 cm/W120

Venetian blinds (2 pieces) 1.5x2.5Office TARGET


Desk chair Leather desk chairBookcases with doors and shelves, with

transparent glass doors Book case H 185 cm / W 75 cm / D 35 cm

Locker cabinet with doors Cabinet H 200 cm / W 105 cm / D = 35 cmSofa for office Leather sofa with 3 places

Table Diverse tableConference table Oval table with 12 places

Executive chairs (12 pieces) Leather chairsPVC door (3 pieces) H 200 cm/W120

Venetian blinds (2 pieces) 1.5x2.5Server hall


Desk with drawer pedestal with 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cmDesk chair (2) Leather desk chair



Vertical rack with ventilation Rack 19” 42UErgonomic chair (2 pieces) Plastic chairs with textile support

PVC door H 200 cm/W120Venetian blinds 1.5x2.5

Telemedicine hallComputer desk with drawer pedestal with 3


Desk with drawer pedestal with 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cmDesk chair (2) Leather desk chair

Sofa Leather sofa with 3 placesTable Diverse table


Locker cabinet with doors Cabinet H 200 cm / W 105 cm / D = 35 cmSimple chairs (2 pieces) Plastic chairs with textile support

Cabinet mask sink Cabinet 4 doors H 70 cm / W 160 cm / D 35 cm, with depositing shelf

PVC door H 200 cm/W120Venetian blinds (2 pieces) 1.5x2.5

Archive space




Necessary furniture Characteristics: D=depth, W=width, H=heightPVC door H 200 cm/W120

Venetian blinds 1.5x2.5Imagistic deposit


PVC door H 200 cm/W120Venetian blinds 1.5x2.5

Access lobbyPVC door H 200 cm/W120

Sanitary groupVentilation installation (2 pieces) Air ventilation 20 mc/min

PVC door H 200 cm/W120Electrical panel + telephone central

PVC door (2 pieces) H 200 cm/W120


The following table presents the minimal characteristics that the special furniture must have for endowment of the laboratories of the Submodule of Endoscopic Surgery

I. Furniture Digestive Imaging ModuleI.3 Endoscopic Surgery Submodule

Necessary furniture Characteristics: D=depth, W=width, H=heightLaboratory of Laparoscopic Surgery

Operation table of veterinary use

- special for veterinary use, allows positioning and fixing experience animal (pig 40-50kg) in supine position

- allows positioning on table right left, inclination Trendelenburg / antiTrendelenburg

Modules for emergency medication with doors from

transparent glass



Pulley with metallic shelves and wheels with self blocking devices

Pulley 1,80m/60cm/30cm with 5 shelves for arrangement of components of tower of video laparoscopy

Venetian blinds (2 pieces) 0,5/1mInstruments table Instruments table, adjustable on height 150/60/30Instruments table Instruments table on self - blocking wheels 90/90/60

N.O.T.E.S. Laboratory

Operation table of veterinary use- special for veterinary use, allows positioning and fixing experience

animal (pig 40-50kg) in supine position- allows positioning on table right left, inclination Trendelenburg /

antiTrendelenburgModules for emergency

medication with doors from transparent glass



Pulley with metallic shelves and wheels with self blocking devices

Pulley 1,80m/60cm/30cm with 5 shelves for arrangement of components of tower of video laparoscopy

Endoscopy cabinet Cabinet with metallic structure H 200 cm / W 105 cm / D = 35 cm, with special ventilation, support for 4 endoscopes

Venetian blinds (2 pieces) 100/180


I. Furniture Digestive Imaging ModuleI.3 Endoscopic Surgery Submodule

Necessary furniture Characteristics: D=depth, W=width, H=heightInstruments table Instruments table, adjustable on height 150/60/30Instruments table Instruments table on self - blocking wheels 90/90/60

Room for personnelExtensible sofa Three places extensible sofa

Conference table Rectangular or oval conference table with length of 2-3m and width of 1 m

Desk chairs Desk chairs with adjustable back and height adjustableComputer desk Desk for computer with 1 gliding shelf for keywords

Shelf Shelf with 3 drawers for desk for computer

Book case Bookcase with shelves, drawers, cases200/400/30 cm

Kitchen cabinetKitchen cabinet with board at height of 90 cm, drawers in the inferior

part and cases with doors in the superior one2/3/0,3 m

Venetian blinds (2 pieces) 100/180Hanger Wall hanger with 6 supports

DisinfectingMobile metallic shelf Metallic shelf 90/90/60 cm on wheels with self-locking device

Autoclave Disinfecting apparatus with moist heatDry heat sterilizer (Pupinel) Disinfecting apparatus with dry heatApparatus for sealing bags Apparatus for sealing bags

Ultrasounds sterilizer Ultrasounds sterilizer apparatusSterilizer oxide of ethylene Sterilizer apparatus with oxide of ethylene

Hand dryer Hand dryer with jet of warm airDispenser Dispenser paper towels

Bathroom mirror Bathroom mirror – 50/30 cmShower booth Corner shower booth with mat glass 180/90Wall hanger Wall hanger with 2 supports

Bath tub Bath tub 90/90 cmCabinet with shelves and drawers Cabinet 200/400/60 cm with multiple cases, shelves, drawers

Sanitary groupToilette chair Toilette chairs with lateral evacuation

Hygienic paper support Wall fixing hygienic paper supportSoap support Wall fixing soap support

Sink Wall fixing sinkSink mask Sink mask – 90/50/30 cm

Shelf ShelfHand dryer Hand dryer with jet of warm airDispenser Dispenser paper towels

Bathroom mirror Bathroom mirror – 50/30 cmShower booth Corner shower booth with mat glass 180/90

Bath tub Poly acryl bath tub 90/90 cmWall hanger Wall hanger with 2 supports

Washstand

Surgical washstand Metallic surgical washstand with depth of 50 cm 2 washing units with warm and cold water

Room for processing instrumentsMetallic sink Metallic sink having depth of 50 cm warm and cold waterMetallic table Metallic table 90/90/60 with shelf

Room for processing biopsiesMetallic table Metallic table 90/90/60 with shelfRefrigerator Refrigerator 150/80/70

Personal vetiary

Cabinet with door Cabinet with door 220/90/60 cm and latch inferior shelves and hanger support



The following table represents the minimal characteristics that the special furniture for the Pathology and Immunology Module must have:

II Furniture Pathology and Immunology ModuleNecessary furniture Characteristics

L=length, l=width, H=heightHistopathology

High cabinet with glass L 120 cm/l 52 cm/H 19,2 cm

Cabinet of chemical products Polypropylene resistant at acidsL 90 cm/l 52 cm/H 72 cm

Extraction kit Kit of accessories to realize the ventilation inside the cabinet

Laboratory special sink Material Inox, deepL 70 cm/l 50 cm/H 66 cm

Pendent cabinet with two doors L 60 cm/ l 36 cm/H 63Pendent cabinet with two doors L 90 cm/ l 36 cm/H 63Pendent cabinet with two doors L 45 cm/ l 36 cm/H 63

Pendent cabinet with two doors of glass L 120 cm/ l 36 cm/H 63Pendent cabinet with two doors of glass L 90 cm/ l 36 cm/H 63

Mobile, rotation chair with back Polyurethane, feet supportAdjustable height 56-82 cm

Venetian blinds 1.5x2.5Immunohistochemistry and Immunocitochemistry

Working place with ceramic surface L 240 cm/ l 75/H 90 cmHigh cabinet with glass L 90 cm/l 52 cm/H 19,2 cm

Special sink for laboratory Material Inox, deepL 70 cm/l 50 cm/H 66 cm

Work office L 150 cm/ l 75 cm /H 75 cm


Mobile, rotation chair with back Leather with elbow supportVenetian blinds 1.5x2.5

Cytology and hybridsWorking place with ceramic surface L 240 cm/ l 75/H 90 cmWorking place with ceramic surface L 120 cm/ l 75/H 90 cm

Pendent cabinet with two doors of glass L 120 cm/ l 36 cm/H 63Metallic cabinet for storage of reactive L 120 cm/l 52 cm/H 19,2 cm

Extraction kit Kit of accessories to realize the ventilation inside the cabinetHigh cabinet with glass L 90 cm/l 52 cm/H 19,2 cmHigh cabinet with glass L 120 cm/l 52 cm/H 19,2 cm

Special sink for laboratory Material Inox, deepL 70 cm/l 50 cm/H 66 cm

Working office L 150 cm/ l 75 cm /H 75 cm



Image computerized imageWorking office L 180 cm/ l 75 cm /H 75 cm

Corner sofaMobile, rotation chair with back Leather with elbow support

Metallic cabinet with sliding doors from temperate glass and four shelves L 180 cm/l 45 cm/H 200 cm

Pendent metallic cabinet with sliding doors from temperate glass and one shelf L 180 cm/l 45 cm/H 74 cm



Venetian blinds 1.5x2.5Aula of Telepathology - Telemedicine



L=length, l=width, H=heightMetallic cabinet with sliding doors from

temperate glass and four shelves L 120 cm/l 45 cm/H 200 cmPendent metallic cabinet with sliding doors

from temperate glass and one shelf L 120 cm/l 45 cm/H 74 cm

Work office L 150 cm/ l 75 cm /H 75 cmMobile, rotation chair with back Leather with elbow support

Venetian blinds 1.5x2.5Laboratory for Preparation of documentary material for formation




Work office L 240 cm/ l 75 cm /H 75 cmWork office L 150 cm/ l 75 cm /H 75 cm


Documents and diagnosis cabinetsWork office L 150 cm/ l 75 cm /H 75 cm

Mobile, rotation chair with back Leather with elbow supportHystotech of high capacity de mare

capacitate L 102,3 cm/ l 72,5 cm /H 145 cm

Venetian blinds 1.5x2.5Secretariat/Server LAN





Rack vertical with ventilation Rack 19” 42U

Cabinet sink mask Cabinet with 4 doors H 70 cm / W 160 cm / D 35 cm, with storage shelf


Venetian blinds 1.5x2.5Deposit of reactive materials

Metallic cabinet for reactive storage L 90 cm/l 52 cm/H 19,2 cmExtraction kit Accessories kit to realize ventilation inside the cabinet

Venetian blinds 1.5x2.5Room for Receiving - registration tests

Working place with ceramic surface L 240 cm/ l 75/H 90 cmWork office L 150 cm/ l 75 cm /H 75 cm


Laboratory Flow citometricBanc de lucru cu suprafata de ceramica L 150 cm/ l 75/H 90 cm




Documentation, Interpretation and Data processing CabinetWork office L 150 cm/ l 75 cm /H 75 cm

Mobile, rotation chair with back Leather with elbow support



L=length, l=width, H=heightMetallic cabinet with sliding doors from


from temperate glass and one shelf L 120 cm/l 45 cm/H 74 cmMetallic cabinet with sliding doors from


from temperate glass and one shelf L 120 cm/l 45 cm/H 74 cm

Venetian blinds 1.5x2.5Laboratory ELISA



The following table represents the minimal characteristics that the special furniture for the Molecular Biology and Biochemistry Module must have:

III Furniture Molecular Biology and Biochemistry ModuleIII.1 Molecular Biology Submodule

Furniture necessary CharacteristicsD=depth, W=width, H=height

Laboratory of cellular culturesWork surface, ceramic shelf Modular (A0214) (Lxl) 1200x700

Work surface, shelf PAL with surface HPL-R (A0202) (Lxl) 1200x700Cabinets under the shelf, 1 place, 4 drawers positioning

HPL (C0103+C0103) (LxlxH) 600x550x660 mm

Cabinets under the shelf, pedestal HPL, 1 place, door, shelf (C0001+C0101) (LxlxH) 600x550x660 mm

Balance table, PAL, Granit board and rubber dampers (L0001) (LxlxH) 900x700x900 mm

Simple desk from Pal, with four drawers (B0021) 1200x700x750 mmNormal laboratory professional chairs (T0011)

Fix laboratory table (N0001) 1200x700x37mmPendent cabinet, PAL, 2 doors and shelf (S0021) (LxlxH) 600x300x700 mmPendent cabinet, PAL, 4 doors and shelf (S0012) (LxlxH) 1200x300x700 mm

Rotation mobile cabinet, 2 places, doors HPL (E0002) 1200x550x690Cabinet PAL, with glass doors in the upper part, PAL

doors in the lower part (D0041)(LxlxH) 800x370x2000 mm

Cloakroom cabinet(D0002)

Dimensions(LxlxH) 400x500x2000 mm

Ceramic double sink with double battery (K0012) (LxlxH) 880x485x322 mmVenetian blinds 1.5x2.5

Cytogenetic laboratory Work surface, modular, ceramic shelf (A0214) (Lxl) 1200x700

Work surface, shelf PAL with surface HPL-R (A0202) (Lxl) 1200x700Cabinets under shelf, 1 place, 4 drawers, pedestal

drawers HPL (C0103+C0103) (LxlxH) 600x550x660 mm

Cabinets under shelf, pedestal HPL,1 place, door, shelf(C0001+C0101) (LxlxH) 600x550x660 mm

Balance table, PAL, Granit board and rubber dampers (L0001) (LxlxH) 900x700x900 mm

Normal laboratory professional chairs (T0011)Fix laboratory table (N0001) 1200x700x37mm

Pendent cabinet, PAL, 2 doors and shelf (S0021) (LxlxH) 600x300x700 mmPendent cabinet, PAL, 4 glass doors and shelf (S0012) (LxlxH) 1200x300x700 mm


III Furniture Molecular Biology and Biochemistry ModuleIII.1 Molecular Biology Submodule

Furniture necessary CharacteristicsD=depth, W=width, H=height

PAL cabinet, with glass doors in the upper part, PAL doors in the lower part (D0041)

(LxlxH) 800x370x2000 mm

Venetian blinds 1.5x2.5

Genomic laboratoryLaboratory professional chair, multiple adjustment (T0021)

Fix laboratory table (N0001) 1200x700x900mm (LxlxH)Modular cabinet with drawers and doors (M002) 1800x700x900

Double, ceramic sink (K0011)Simple desk from pal, with four drawers (B0021) 1200x700x750

Balance table, PAL, Granit board and rubber dampers (L0001) 900x700x900

PAL Cloakroom cabinet (D0002) 400x500x2000Cabinet from PAL, doors, rail drawers (D0081) 800x370x2000

Cabinet from PAL, glass doors, aluminum frame, two rail shelves (D0051) 800x370x2000

Pendent cabinet, PAL, 4 doors and shelf (S0012) 1200x300x700Rotation mobile cabinet, 2 places, HPL doors (E0002) 1200x550x690Rotation, mobile cabinet, 1 place, doors HPL (E0003),

drawers HPL (E0023) 600x550x690

Cabinet from pedestal shelf HPL 1 place, door, shelf (C0001 + C0101) 600x550x660

Cabinet for shelf1 place, 4 drawers, pedestal HPL (C003+C0103) 600x550x660

Shelf HPL-R (for chemistry, highly chemical resistant) (A0302) 1800x700

White modular ceramic shelf (A0314) 1800x700Venetian blinds 1.5x2.5

Proteomic laboratoryLaboratory professional chair, multiple adjustment (T0021)

Fix, laboratory table (N0001) 1200x700x900Middle table with gases and sockets and sink at the

bottom (M0001)1800x700

extremities 1400x700Ceramic rectangular sink (K0002)

Simple desk from PAL, with four drawers (B0021) 1200x700x750Balance table, PAL, granite shelf and rubber dampers

(L0001) 900x700x900

Cabinet cloakroom from PAL (D0002) 400x500x2000

Cabinet from PAL doors (D0011) 800x350x2000

Cabinet from PAL, glass doors, aluminum frame (up), two selves with rail down (D0051) 800x370x2000

Pendent cabinet, PAL, 4 doors and shelf (S0022) 1200x300x700

Mobile cabinet with castors, 2 places, doors HPL (E0022) 1200x550x690

Mobile cabinet with castors, 1 place, doors HPL (E0003), drawers HPL (E0023) 600x550x690

Cabinet for pedestal shelf HPL 1 place, door, shelf (C0001 + C0101) 600x550x660

Cabinet for shelf, 1 place, 4 drawers, pedestal HPL (C003+C0103) 600x550x660

Shelf HPL-R (for chemistry, highly chemical resistant) (A0302) 1800x700

White modular ceramic shelf 3 places(A0314) 1800x700Venetian blinds 1.5x2.5


III Furniture Molecular Biology and Biochemistry ModuleIII.2 Biochemistry Submodule

Furniture necessary CharacteristicsL=length, l=width, H=height

Laboratory for processing testsHigh cabinet with window L 120 cm/l 52 cm/H 19,2 cm

Cabinet of chemical products Polypropylene resistant at acidsL 90 cm/l 52 cm/H 72 cm

Laboratory special sink Material Inox, deepL 70 cm/l 50 cm/H 66 cm

Laboratory professional chair, multiple adjustment (T0021)Fix, laboratory table (N0001) 1200x700x900

Middle table with gases and sockets and sink at the bottom (M0001)

1800x700extremities 1400x700

Ceramic rectangular sink (K0002)Simple desk from PAL, with four drawers (B0021) 1200x700x750

Balance table, PAL, granite shelf and rubber dampers (L0001)

900x700x900

Cabinet cloakroom from PAL (D0002) 400x500x2000Cabinet from PAL doors (D0011) 800x350x2000

Cabinet from PAL, glass doors, aluminum frame (up), two selves with rail down (D0051)

800x370x2000

Pendent cabinet, PAL, 4 doors and shelf (S0022) 1200x300x700Mobile cabinet with castors, 2 places, doors (E0022) 1200x550x690

Mobile cabinet with castors, 1 place, HPL doors (E0003), drawers HPL (E0023)

600x550x690

Cabinet for pedestal shelf HPL 1 place, door, shelf (C0001 + C0101)

600x550x660

Cabinet for shelf, 1 place, 4 drawers, pedestal HPL (C003+C0103)

600x550x660

Shelf HPL-R (for chemistry, highly chemical resistant) (A0302)

1800x700

White modular ceramic shelf 3 places(A0314) 1800x700Venetian blinds 1.5x2.5

Secretariat / Server LANMetallic cabinet with sliding doors from temperate glass and four

shelvesL 120 cm/l 45 cm/H 200 cm

Pendent metallic cabinet with sliding doors from temperate glass and one shelf

L 120 cm/l 45 cm/H 74 cm

Metallic cabinet with sliding doors from temperate glass and four shelves

L 120 cm/l 45 cm/H 200 cm


L 120 cm/l 45 cm/H 74 cm


Venetian blinds 1.5x2.5Deposit reactive materials

Metallic cabinet for reactive storage L 90 cm/l 52 cm/H 19,2 cmMetallic cabinet for reactive storage L 90 cm/l 52 cm/H 19,2 cm

Extraction kit Accessories kit to realize ventilation inside cabinet

Venetian blinds 1.5x2.5Laboratory Microscopy Confocal with two photons

Optical table L 200 cm/ l 100/H 90 cmWork space with ceramic surface L 150 cm/ l 75/H 90 cm

Work office L 150 cm/ l 75 cm /H 75 cmMobile, rotation chair with back Polyurethane, feet support

Adjustable height 56-82 cm


III Furniture Molecular Biology and Biochemistry ModuleIII.2 Biochemistry Submodule

Furniture necessary CharacteristicsL=length, l=width, H=height


Laboratory for analyze MALDI-TOFHigh cabinet with window L 120 cm/l 52 cm/H 19,2 cmCabinet chemical products Polypropylene resistant at acids

L 90 cm/l 52 cm/H 72 cmLaboratory special sink Material Inox, deep

L 70 cm/l 50 cm/H 66 cmPendent cabinet with two glass doors x 3 L 60 cm/ l 36 cm/H 63Pendent cabinet with two glass doors x2 L 120 cm/ l 36 cm/H 63

Work place with ceramic surface L 240 cm/ l 75/H 90 cmLuminescence Laboratory

Work office L 180 cm/ l 75 cm /H 75 cmCorner sofa

Mobile, rotation chair with back Leather with elbow supportMetallic cabinet with sliding doors from temperate glass and four



L 180 cm/l 45 cm/H 74 cm


L 150 cm/l 45 cm/H 200 cm


L 150 cm/l 45 cm/H 74 cm

Venetian blinds 1.5x2.5Laboratory of Microscopy with atomic force

Work office L 180 cm/ l 75 cm /H 75 cmCorner sofa

Mobile, rotation chair with back Leather with elbow supportMetallic cabinet with sliding doors from temperate glass and four



L 180 cm/l 45 cm/H 74 cm


L 150 cm/l 45 cm/H 200 cm


L 150 cm/l 45 cm/H 74 cm

Venetian blinds 1.5x2.5

The following table presents the minimal characteristics that the special furniture must have for Telemedicine and Artificial Intelligence Module:

Necessary furniture CharacteristicsH=height, W=width, D=depth

Aula of servers TCOMComputer desk with drawer pedestal and 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cmDesk with drawer pedestal and 3 drawers (3 pieces) Simple desk H 75 cm / W 160 cm / D 80 cm

Office chair (2 pieces) Leather office chairMobile, rotation chair with back (2 pieces) Leather with elbow support

Bookshelves with doors and shelves, with transparent glass doors (2 pieces) Bookshelf H 185 cm / W 75 cm / D 35 cm

Pendant cabinets with doors and drawers (6 pieces)Cabinet with doors in the superior part and drawers in the inferior part: H 220 cm/ W 75

cm / D 35 cmRack vertical with ventilation Rack 19” 42U



Ergonomic chair (2 pieces) Plastic chairs with textile supportVestiary cabinets with doors Cabinet H 200 cm / W 105 cm / D = 35 cm

Ventilation installation Air ventilation 20 mc/minPVC door (2 pieces) H 200/W120

Alarm and surveillance system Surveillance, security and data basisCode lock/ card Security electrical net and communication

Venetian blinds (2 pieces) 1.5x2.5Aula UPS

Desk with drawer pedestal and 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cmMobile, rotation chair with back Leather with elbow support


cm / D 35 cmPVC door H 200/W120

Ventilation installation Air ventilation 60 mc/minVenetian blinds (2 pieces) 1.5x2.5

Deposit

Pendant clavinets with doors and drawers (4 pieces)Cabinet with doors in the superior part and drawers in the inferior part: H 220 cm/ W 75


Ventilation installation Air ventilation 20 mc/minLobby

PVC door H 200/W120Aula of administration data basis (SGDB)

Computer desk with drawer pedestal and 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cmDesk with drawer pedestal and 3 drawers (3 pieces) Simple desk H 75 cm / W 160 cm / D 80 cm

Office chair (2 pieces) Leather office chairMobile, rotation chair with back (2 pieces) Leather with elbow support

Metallic cabinet with sliding doors from temperate bottle and four shelves (12 pieces) L 180 cm/l 45 cm/H 200 cm

Metallic pendant with sliding doors from temperate glass and one shelf (12 pieces) L 180 cm/l 45 cm/H 74 cm

Bookshelves with doors and shelves, with transparent doors from glass (2 pieces) Bookshelf H 185 cm / W 75 cm / D 35 cm


cm / D 35 cmLocker cabinet with doors Cabinet H 200 cm / W 105 cm / D = 35 cm

Cabinet sink mask Cabinet 4 doors H 70 cm / W 160 cm / D 35 cm, with storage shelf

Alarm and surveillance system Surveillance, security and data basisCode lock/ card Security data basis

PVC door (3 pieces) H 200/W120Ventilation installation Air ventilation 20 mc/min

Command aula SCComputer desk with drawer pedestal and 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cmDesk with drawer pedestal and 3 drawers (2 pieces) Simple desk H 75 cm / W 160 cm / D 80 cm

Office chair (4 pieces) Leather office chairBookshelves with doors and shelves, with transparent

glass doors (2 pieces) Bookshelf H 185 cm / W 75 cm / D 35 cm

Ergonomic chair (2 pieces) Plastic chair with textile supportSofa Leather sofa with three placesTable Diverse table

PVC door H 200/W120Archive

Desk with drawer pedestal and 3 drawers Simple desk H 75 cm / W 160 cm / D 80 cmOffice chair Leather office chair





Exterior metallic door H 200/W120Sub module Artificial Inteligence and Ststistic Processing

Computer desk with drawer pedestal and 3 drawers (2 pieces) Simple desk H 75 cm / W 160 cm / D 80 cm

Desk with drawer pedestal and 3 drawers (2 pieces) Simple desk H 75 cm / W 160 cm / D 80 cmOffice chair (2 pieces) Leather office chair

Ergonomic chair (2 pieces) Plastic chair with textile supportBookshelves with doors and shelves, with transparent

glass doors (2 pieces) Bookshelf H 185 cm / W 75 cm / D 35 cm

Pendant cabinets with doors and drawersCabinet with doors in the superior part and drawers in the inferior part: H 220 cm/ W 75


Venetian blinds (2 pieces) 1.5x2.5

The acquisition of the necessary infrastructure for the Research Centre „TARGET” will ignite the research directions which were proposed in the frame of the project. The results of the research may reduce human, financial and material losses due to a wrong diagnosis. They also may improve the health status of the population. Those acquisitions are practically ways to improve efficiency of applicative activities and consequently they will improve the economic efficiency. In this manner, the project is related to the main requirement of the Sectoral Operational Program for increasing the Economic Efficiency.The Educational System from Romania will also benefits from the techniques and devices achieved in the frame of the project by offering to a large number of students and young PhD students a fair chance to improve their skills and to fulfil their studies.The results of the conducted research will offer to the society of the underprivileged Region of OLTENIA a better access to a fair and efficient treatment and diagnostic.


2.c Technical data of investment

2.c.1 Area and location

LOCATION AOld Building of UMF Craiova, Petru Rares Street, No 2, Craiova – 200349

LOCATION BNew Building of UMF Craiova, 1 Mai Boulevard, No 66, Craiova – 200638

2.c.2 Judicial status of the ground

The ground belongs to University of Medicine and Pharmacy due to Ministry of Education Order No. 3388/10.03.2008

2.c.3 Situation of definite ground occupations

Order No. 3388/10.03.2008 concerning propriety rights.

2.c.4 Ground studies

Ground studies are attached in annex 9.

2.c.5 Main characteristics of constructions inside investments objective and the constructuve variants of investment’s achievement

Constructive opion

An alternative constructive variant to the one presented in the architecture memorial, can be the following:1. STRUCTURAL SYSTEM-constructive system: foundations in ferro-concrete, pillars and metallic beams, plate floors and BA; vertical circulation will be completely achieve by BA diaphragms;2. CLOSINGS AND DIVISIONS2.1. Closings - Curtain facade on aluminum structure- Opaque closings with veneering on ventilated structure2.2 DivisionsInternal divisions are achieved of easy walls, unstructured, made of gyps-cardboard partition walls with acoustic treatment of 15 cm.3. EXTERNAL AND INETRNAL FINISHINGS3.1. External finishingBase: decorative plaster and veneering with natural stone;Walls: curtain wall on aluminum structure and veneering on ventilated structure;Joiner’s trade: aluminum double glazing doors and windows, provided with tearing of thermal bridge;Flat Covering: thermo-insulating and hydro-insulating3.2. Internal finishingFloors: intense traffic carpet for offices, -ceramics or natural stone for walls, sanitary groups and stairs, -PVC antistatic and antibacterial floor - in labs.Studiu de fezabilitate – TARGET SF 101

Walls: washable painting on gyps cardboard veneering and false divided ceiling.Ceilings: washable painting with gyps cardboard floors and divided false floor Joinery: wooden joinery (cellular plate doors (massive wood-furnishings)), - aluminum joinery with double glazing windows).This constructive variant has the advantage of a faster execution time, then the variant presented in the technical memo, but it has a series of disadvantages:- increased of the execution cost according to the requests of the Law no. 10/1995;- thermal and waterproof protection and energy economy- noise protection;- special measurements of labor protection, during construction development;- needs of special measurements against fires;- complex and expensive solutions for foundations and brickworks, in order to fulfill earthquake protection standards in Romania.Taking into account all these facts the constructive solution presented in the architectural memo is the most suitable.

Technological option

From the analysis were taken into account only those devices/devices systems which Are not unique, so those which can make the object of an option analysis. For the time, the selection of technological optimal choice can be only made for some of these equipments/systems, the rest being unique on the market.In case of device/system which are not unique, in order to choose the optimal alternatives, were taken into account the establishing of some minimal technical functioning parameters and the advantages which they present: device/system, performance of the parameters interested for the searchers, as well as estimated cost of the device/system. Finally, each device/system received a score. The device/system which has the best score was chosen as being optimal to the rigging of TARGET infrastructure.The following equipments will make the object of an option analysis:-PET-CT combined system, proposed for acquisition within the Digestive Imaging Module;- MRI 3T system, proposed for acquisition within the Digestive Imaging Modules;- Completion of autofluorescence system (AFI) + magnification (MCE) + NBI mood, proposed for acquisition within the Digestive Imaging Modules;- Laser induced fluorescent spectroscopy system (LIFS), proposed for acquisition within the Digestive Imaging Modules; - Completing confocal laser endomicroscopy system (CLE), proposed for acquisition within the Digestive Imaging Modules; - Laser confocal endomicroscopy miniprobe system, proposed for acquisition within the Digestive Imaging Modules;- System for gene expression evaluation through microarray technology, proposed for acquisition within the Molecular Biology and Biochemistry Modules;- System for gene expression evaluation through qReal-time PCR, proposed for acquisition within the Molecular Biology and Biochemistry Modules;- AFM Atomic force microscope system, proposed for acquisition within the Molecular Biology and Biochemistry Modules;Studiu de fezabilitate – TARGET SF 102

- 2 photons confocal fluorescence microscopy system, proposed for acquisition within the Molecular Biology and Biochemistry Modules;

The following tables present the analyses for the optimal choice for the devices/equipments proposed to be suitable for the above research activities. In the analyses performing were taken into account those performances and criteria which must be fulfilled by the devices and systems in order to correspond to the proposed research activities (best technical characteristics according with researchers option were given maximal 5 points). In order to establish economical performances were taken into account the main characteristics which influenced the investment decision. (best characteristics according with researchers option were given maximal 5 points). The environmental impact was analyzed as a function of the interaction between the device/system and environment. Strong, negative influence of he devices/systems on the environment were poorly scored.Legal request conformity: maximal score were given to those devices/equipments homologated.


OPTIMAL OPTIONS TABLEanalyzed system/device

Option 1 Pt Option 2 Pt Option 3 Pt Max Proportion

PET-CT combined system

PET coventional system

Multislice CT conventional system

1. Technical standards (minimal requirement parameters)a. CT (slices/ acquisition) YES(6) 3 NO 0 YES (6) 3 3 30%

b. PET (crystals type, crystal dimensions, axial FOV)

YES(LSO; 4.0 x 4.0 m; 21,6 cm)

3

YES (GSO; 4.0 x 6.0 mm; 18 cm)

2 NO 0 3 30%

c. Gantry aperture 70 cm 0 85 cm 1 70 cm 0 1 10%d. 3D acquisition YES 1 YES 1 YES 1 1 10%e. Virtual colonoscopy software

YES 2 YES 0 YES 2 2 20%

2. Economic criteriaa. Investment costs YES 2 YES 2 YES 2 2 20%b. Administrating costs YES 2 YES 2 YES 2 2 20%

c. Price HIGH 1 HIGH 1 MEDIUM 2 2 20%d. Provenience EU 2 EU 2 EU 2 2 20%

e. Observation NO 2 NO 2 NO 2 2 20%

3. Environment impactYES/NO YES 5 YES 5 YES 5 5 100%

4. Legal requirements complianceStandards, Laws, Regulations, etc

YES (cleared) 5 YES

(cleared) 5 YES (cleared) 5 5 100%

Total score 28 23 26 30Score: 1=very poor, 2=poor, 3=acceptable, 4=good, 5=very good

Of the three variants, variant 1 is optimal, obtaining a maximum score of 28 points, while variant 2 and 3 obtained 23, respectively 26 points


OPTIMAL OPTIONS TABLEanalyzed system/device


Magnetic Resonance System 3T

Magnetic Resonance System 1.5T

Magnetic Resonance System 1.2T (open)

1. Technical standards (minimal requirement parameters)a. Magnetic strength field

Very High (3T) 4 High (1.5T) 2 Medium

(1.2T) 1 4 40%

b. Gantry configuration

Closed-long (>1.5 m) 0

Closed – schort (<1.5 m)

0 Open 1 1 10%

c. Gantry diameter 70 cm 1 60 cm 0 Open 1 1 10%

d. Total imaging matrix YES (TIM) 2 YES(TIM) 2 NO 0 2 20%

e. Virtual colonoscopy software

YES 2 YES 2 NO 0 2 20%

2. Economic criteria a. Investment costs YES 2 YES 2 YES 2 2 20%

b. Administrating costs YES 2 YES 2 YES 2 2 20%

c. Price HIGH 1 HIGH 1 MEDIUM 2 2 20%d. Provenience EU 2 EU 2 EU 2 2 20% e. Observation NO 2 NO 2 NO 2 2 20%

3. Environment impact a. YES/NO NO 5 NO 5 NO 5 5 100%

4. Legal requirements compliance Standards, Laws, Regulations, etc

YES (cleared)

5 YES (cleared)

5 YES (cleared) 5 5 100%

Total score 28 25 23 30 Score: 1=very poor, 2=poor, 3=acceptable, 4=good, 5=very good

Of the three variants, variant 1 is optimal, obtaining a maximum score of 28 points, while variant 2 and 3 obtained 25, respectively 23 points


OPTIMAL OPTIONS TABLEAutofluorescence System (AFI) + Magnification (MCE) + NBI mode

Option 1 pt Option 2 Pt Max pt Proportion

Autofluorescence (AFI) +

magnification (MCE) + NBI mode

White-light endoscopy

1. Technical Criteria (minimal required parameters)a. Resolution High 2 High 2 2 20%b. Structure visualization YES (MCE, 1.5x) 2 Partially 1 2 20%

c. Vascularisation visualization YES (NBI) 1 NO 0 1 10%

d. Real-time diagnosis YES (MCE, NBI) 3 NO 0 3 30%

e. Other Independent system 2 Independent system 2 2 20%

2. Economic criteriaa. Investment costs NO 2 NO 2 2 20%b. Operating costs NO 2 NO 2 2 20%c. Price High 1 Medium 2 2 20%d. Made in EU 2 EU 2 2 20%e. Other NO 2 NO 2 2 20%

3. Environment impacta. YES/NO NO 5 NO 5 5 100%

4. Legal requirements complianceStandards, guides, laws, HG, OG, OM, etc

YES (cleared) 5 YES (cleared) 5 5 100%

Total score 29 25 30Score: 1=very poor, 2=poor, 3=acceptable, 4=good, 5=very good

Of the two options, the optimal is option 1, with an almost perfect 29 points out of 30, while option 2 only received 25, being a conventional method for clinical use, but without utility in research


OPTIMAL OPTIONS TABLELaser induced fluorescent spectroscopy system (LIFS)

Option 1 pt Varianta 2 pt Max pts Proportion

Laser induced fluorescent

spectroscopy (LIFS) White-light endoscopy

1. Technical Criteria (minimal required parameters)a. Resolution Low 1 High 2 2 20%b. Structure visualization NO 2 Partial 1 2 20%

c. Vascularisation visualization NO 1 NO 0 1 10%

d. Real-time diagnosis YES 3 NO 0 3 30%









OPTIMAL OPTIONS TABLEConfocal laser endomicroscopy system

Option 1 pt Option 2 pt Max pts Proportion

Dedicated confocal laser endomicroscopy system White-light endoscopy

1. Technical Criteria (minimal required parameters)a. Resolution Very high 2 High 2 2 20%b. Structure visualization YES (1000x) 2 Partial 1 2 20%

c. Vascularisation visualization YES (fluoresceine) 1 NO 0 1 10%










OPTIMAL OPTIONS TABLELaser induced fluorescent spectroscopy system (LIFS)

Option 1 pt Option 2 pt Max pts Proportion

Confocal laser endomicroscopic probe system

White-light endoscopy

1. Technical Criteria (minimal required parameters)a. Resolution Very high 2 High 2 2 20%b. Structure visualization YES (800x) 2 Partial 1 2 20%

c. Vascularisation visualization YES 1 NO 0 1 10%



2. Economical criteriaa. Investment costs NO 2 NO 2 2 20%b. Operating costs NO 2 NO 2 2 20%c. Price High 1 Medium 2 2 20%d. Made in EU 2 EU 2 2 20%e. Other NO 2 NO 2 2 20%

3. Environmental impacta. YES/NO NO 5 NO 5 5 100%






OPTIMAL OPTIONS TABLEDedicated system for gene expression evaluation - high density microarray

Option 1 Pt Option 2 Pt Max Proportion

Dedicated system for gene expression evaluation - high density microarray

Dedicated system for gene expression evaluation - low density microarray

1. Technical standards (minimal requirement parameters)a. Number of genes simultaneously analyzed

Very high 2 High 1 2 20%

b. importance as a method for gene expression: screening & data validation


c. Specificity YES 1 YES 1 2 20%d. Real-Time evaluation YES 2 YES 0 2 20%

e. Observations

This system includes:- GeneChip Scanner;- Fluidics Station;- Hybridization Oven;- a powerful computer workstation with dual Xeon processors loaded with GeneChip Operating Software.

2

This system includes:- Dedicated scanner for nylon membrane; - Hybridization Oven;- Workstation with dedicated software;

2 2 20%

2. Economic criteriaa. Investment costs NO 2 NO 2 2 20%

b. Administrating costs NO 2 NO 2 2 20%

c. Price High 1 MEDIUM 2 2 20%d. Provenience UE 2 UE 2 2 20%e. Observation NO 2 NO 2 2 20%

3. Enviroment impactYES/NO NO 5 NO 5 5 100%

4. Legal requirements complianceStandards, Laws, Regulations, etc YES (cleared) 5 YES (cleared) 5 5 100%


Of the two variants, 1 is optimal, obtaining a maximum score of 28 points, while variant 2 received 25 points.


OPTIMAL OPTIONS TABLEDedicated system for gene expression evaluation – qReal-Time PCR

Option 1 Pt Option 2 Pt Max Proportion

Dedicated system for gene expression evaluation – qReal-Time PCR

Dedicated system for gene expression evaluation - low density microarray

1. Technical standards (minimal requirement parameters)a. Number of genes simultaneously analyzed

High 1 High 1 2 20%

b. importance as a method for gene expression: screening & data validation


c. Specificity YES 2 YES 1 2 20%d. Real-Time evaluation YES 2 YES 0 2 20%

e. Observations

This system includes:- Automated pipetting system;- Real Time PCR thermalcycler;

2

This system includes:- Dedicated scanner for nylon membrane; - Hybridization Oven;- Workstation with dedicated software;

2 2 20%


b. Administrating costs NO 2 NO 2 2 20%

c. Price HIGH 1 MEDIUM 2 2 20%d. Provenience UE 2 UE 2 2 20%e. Observation NU 2 NO 2 2 20%

3. Enviroment impactYES/NO NO 5 NO 5 5 100%

4. Legal requirements complianceStandards, Laws, Regulations, etc YES (cleared) 5 YES (cleared) 5 5 100%


Of the two variants, 1 is optimal, obtaining a maximum score of 28 points, while variant 2 received 25 points.


OPTIMAL OPTIONS TABLEAtomic force microscope AFM


Atomic force microscope AFM

Scanning Electron microscope SEM

1. Technical standards (minimal requirement parameters)

a. ResolutionHigh (nm-tenth of nm) 2.5 High (nm-

tenth of nm) 2.5 2.5 25%

b. Nanoscale sampling

YES, proteins 2.5 YES, proteins 2.5 2.5 25%

c. Sample preparation YES, normal 2 YES, special 0 2 20%

d. Viscoelasticity measurements YES 1 NO 0 1 10%

e. CommentsIndependent system 2 Independent

system 2 2 20%


b. Administrating costs YES 1 YES 1 2 20%

c. Price Large 1 Very large 0 2 20%d. Provenience UE 2 UE 2 2 20%e. Observation NO 2 NO 2 2 20%





Between the two options, option number 1 is better, with a score of 38 points while the second option has only 34 points.


OPTIMAL OPTIONS TABLETwo-photon microscope


Fluorescent microscope ( Hg lamp)

Confocal microscope

Two-photon microscope

1. Technical standards (minimal requirement parameters)a. Resolution poor 0 high 2 high 2 2 20%b. Sample degradation YES 0 Partial 1 NO 3 3 30%

c. Penetration deep low 1 medium 1 high 3 3 30%

d. Real time 3D imaging No 0 YES 3 YES 2 2 20%

2. Economic criteriaa. Investments expenses NO 2 NO 2 NO 2 2 20%

b. Operation costs NO 2 YES 2 YES 2 2 20%

c. Price small 1 medium 1 High 2 2 20%d. Origin UE 2 UE 2 UE 2 2 20%

3. Environment impacta. Yes/No NO 5 NO 5 NO 5 5 100%


YES (cleared) 5 YES

(cleared) 5 YES (cleared) 5 5 100%

Total score 18 24 28 28Score: 1=very poor, 2=poor, 3=acceptable, 4=good, 5=very good

Among the three options, third one is better with a score of 28 points, while options 1 and 2 have only 18 and 24 points respectively.


2.c.6 Existent situation of utility and consumption analysis

Necessary facilities will be provided by the local suppliers as following: The electricity is distributed by the local provider CEZ Oltenia; connection may be

located either from the County Hospital (about 50m away) or from UMF (about 200m away).

Domestic water and sewerage were provided by local distributors (about 50 meters away)

Gas will be provided by local distributor DISTRIGAZ (20m away) Heating will be provided by UMF thermal heater system Internet facilities will be provided through an optical fiber connection.

2.c.7 Conclusions of evaluation impact over environment

Because through the city planning certificate an Environment Agreement is not requested, and the Law 111/1996 specified that the special certificates would be obtained only after the implementation of the project, it was considered that for this project the assessment it will be realized only if it will be requested by the authorities.


2.d Timeframe and main activities; activities diagram of the investment

Activities diagram

Crt.No. Activities Nr

luniYear 1 Year 2 Year 3 Budget Alocation

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 An 1 An 2 An 3

A1 Administrative activities of the project

A1.1 The project management 35 # # # # # # # # # 1 1 1 1 1 1 1

1 1 1 1 1 1

1 1 1 1 1 1 1 1 1

1 1 1 1 34% 34% 31%

A1.2Preparing and approval of the BD for the selection of a consultant for BD preparing and the assistance during the project management

1

# 100% 0% 0%

A1.3Selection of a consultant for the BD preparation for Services/goods/utilities acquisition and the assistance during the project implementation

3 # # # 100% 0% 0%

A1.4 Preparing and approval of bidding documents for creating an Imaging Module as "ready to go" 1

# 100% 0% 0%

A1.5 Preparing and approving the bidding documents for the equipment procurement 1

# 100% 0% 0%

A1.6 Preparing and approving the bidding documents for building improvements 1

# 100% 0% 0%

A1.7 Preparing and approving bidding documents for advertising services 1

# 100% 0% 0%A1.8 The selection of a financial auditor 1 0% 0% 100%A1.9 The execution of financial audit 1 0% 0% 100%

2 The creation of the TARGET center infrastructure

2.a Construction Imagistic building as "ready to go"

2.a1 The carrying on of the bidding procedure for an "ready to go" project of the imagistic building 4

# # # # 100% 0% 0%

2.a2 The construction, the improvement and the rigging of the Imagistic Building as "ready to go" 21

1 1 1 1 1 1 1 1 1 1 1 1 1

1 1 1 1 1 1 1 1 14% 57% 29%

2.a3 The final reception and commissioning of the Imagistic building as "ready to go" 1 1

2.b The improvement of the existent facilities

2.b1 The carrying on of the works bidding procedure & the selection of the works executants 4

# # 1 1 100% 0% 0%

2.b2 The execution of the improvements works 12 1 1 1 1 1 1 1 1 1 1 1

1 8% 92% 0%

2.b3 The reception of the works 1 1 0% 100% 0% 3 The rigging of TARGET module 0

3.1 The carrying on of the goods bidding procedure & the selection of the goods suppliers 4

1 1 1 1 0% 100% 0%

3.2 The delivery of the equipment and the furniture 11 1 1 1 1 1 1 1 1 1 1 1 0% 0% 100%

3.3 Installing, testing and reception 5.5 1 1 1 1 1 1 1 1 1 1 1 0% 0% 100%

3.4 Staff training regarding the use the new infrastructure 5.5

1 1 1 1 1 1 1 1 1 1 1 0% 0% 100%

0 4 The administrative ability reinforcement 0

4.a The acquisition of goods for the reinforcement of the administrative capacity 0

4.a1The carrying on of the bidding procedure of goods and the selection of the goods supplier/suppliers

2 # # 100% 0% 0%

4.a2 The delivery of the goods 4 # # # # 100% 0% 0%4.a3 Installing, testing and reception 0.4 # # # # 100% 0% 0%

4.a4 Staff training regarding using the new infrastructure 0.4

# # # # 100% 0% 0%


0

4.b Advertising 0

4.b1 The selection of the advertising services supplier 0.5 # 100% 0% 0%

4.b2 The manufacture and the application of the marks on the purchased equipments 2

1 1 0% 0% 100%4.b3 The realization of audio-video spots # # # 4.b4 The fulfiling of posters and brochures 3 # # # 100% 0% 0%

4.b5 The distribution of posters and brochures 2.7 # 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0

0 0 0 0 15% 44% 41%

Legend: The activity will carry on continually, during the mentioned period of time The activity will carry on during the mentioned period of time, but not constantly The activity will carry on in agreement with the needs/opportuneness in the mentioned period of time


3 ESTIMATED COST OF INVESTMENT

3.a Total value including the detalied presentation of the general estimate structureThe total value of the investment for the proposed project „TARGET – CENTRE FOR TREATMENT AND RESEARCH IN GASTROENTEROLOGY BASED ON IMAGING METHODS AND MOLECULAR TECHNIQUES” amounts to 70.331,13 thousands RON VAT included and involves expenses for the construction of an Excellence Research Centre (including a new IMAGING DEPARTMENT Building), the improvement of the existing spaces, as well as the improvement of research-development infrastructure.

The total expenses of the proposed investment objective is presented according to the new structure of the general estimate, as follows:GENERAL ESTIMATE in compliance with the Government Resolution no. 28/2008 with reference to the expenses necessary for

the achievement of "TARGET – CENTRE FOR TREATMENT AND RESEARCH IN GASTROENTEROLOGY BASED ON IMAGING METHODS AND MOLECULAR TECHNIQUES " in thousands of LEI/ thousands of Euros

Exchange rate 3.6Lei/Euro

Current Number

Designation of chapters and subchapters and of the expenses’

subchapters

Value (VAT excluded) VAT Value ( VAT included)Thousands of

leiThousands of

euroThousands of

leiThousands

of leiThousands

of euro1 2 3 4 5 6 7

I CHAPTER: Expenses procurement and arrangement of land

1.1 Procurement of land - - - - -

1.2 Arrangement of land 84.60 23.50 16.07 100.67 27.97

1.3

Arrangements for the environment’s protection and bringing it to the initial state - - -

- -

TOTAL I CHAPTER 84.60 23.50 16.07 100.67 27.97

2 II CHAPTER: Expenses for the provision of utilities necessary for the objective

2.1Expenses for the provision of utilities necessary for the objective 36.00 10.00 6.84 42.84 11.90

TOTAL II CHAPTER 36.00 10.00 6.84 42.84 11.90 III CHAPTER: Expenses for the planning and technical assistance


GENERAL ESTIMATE in compliance with the Government Resolution no. 28/2008 with reference to the expenses necessary for the achievement of "TARGET – CENTRE FOR TREATMENT AND RESEARCH IN GASTROENTEROLOGY BASED ON IMAGING

METHODS AND MOLECULAR TECHNIQUES " in thousands of LEI/ thousands of Euros Exchange rate 3.6

Lei/Euro

Current Number


subchapters


leiThousands of

euroThousands of

leiThousands

of leiThousands

of euro1 2 3 4 5 6 7

3.1 Land Surveys 7.56 2.10 1.44 9.00 2.50

3.2Taxes for the procurement of approvals, permits and authorizations 166.53 46.26 31.64 198.17 55.05

3.3 Planning and engineering 460.59 127.94 87.51 548.10 152.25

3.4Organization of the acquisition procedure 12.86 3.57 2.44 15.30 4.25

3.5 Consultancy 1,102.94 306.37 25.76 1,128.71 313.533.6 Technical assistance 13.22 3.67 2.51 15.73 4.37

TOTAL III CHAPTER 1,763.71 489.92 151.31 1,915.01 531.95 IV CHAPTER: Expenses for the basis investment

4.1 Constructions and Installations 15,810.24 4,391.73 3,003.94 18,814.18 5,226.164.2 Assembly of technological outfits 47.52 13.20 9.03 56.55 15.71

4.3Equipment, technological and functional outfits with assembly 36,422.28 10,117.30 6,920.23 43,342.51 12,039.59

4.4Equipment without assembly and transportation outfits 32.40 9.00 6.16 38.56 10.71

4.5 Endowments 1,898.46 527.35 360.71 2,259.17 627.554.6 External assets 227.70 63.25 43.26 270.96 75.27

TOTAL IV CHAPTER 54,438.60 15,121.83 10,343.33 64,781.93 17,994.98 V CHAPTER: Other expenses

5.1 Site organization 455.57 126.55 86.56 542.13 150.595.1.1 Construction works 373.81 103.84 71.02 444.83 123.56

5.1.2Expenses connected to the site organization 81.76 22.71 15.53 97.30 27.03

5.2Commissions, shares, fees, cost of credit 212.58 59.05 0.00 212.58 59.05

5.2.1 Commissions, shares, legal fees 212.58 59.05 0.00 212.58 59.05


GENERAL ESTIMATE in compliance with the Government Resolution no. 28/2008 with reference to the expenses necessary for the achievement of "TARGET – CENTRE FOR TREATMENT AND RESEARCH IN GASTROENTEROLOGY BASED ON IMAGING

METHODS AND MOLECULAR TECHNIQUES " in thousands of LEI/ thousands of Euros Exchange rate 3.6

Lei/Euro

Current Number


subchapters


leiThousands of

euroThousands of

leiThousands

of leiThousands

of euro1 2 3 4 5 6 7

5.2.1.1

Concerned share of the State Construction Inspectorate ((0.7%+0.1%) x (C+M)) 130.82 36.34 - 130.82 36.34

5.2.1.2Concerned share of the Constructors’ Social Fund (0.5% x (C+M)) 81.76 22.71 - 81.76 22.71

5.2.1.3Fees, permits, approvals and building license (1% x (C+M)) - - - - -

5,2,2Cost of credit (0.1% x

(1.2+1.3+2+3+4+5.1+5.2.1)) - - - - -

5.3

Miscellaneous and unforeseen expenses (max.10% x (1.2+1.3+2+3+4)) 2,252.92 625.81 428.05 2,680.97 744.71

TOTAL V CHAPTER 2,921.06 811.41 514.61 3,435.68 954.35 VI CHAPTER: Expenses for technological tests, testing and handing over to the beneficiary

6.1 Training of operation personnel 29.41 8.17 5.59 35.00 9.726.2 Technological tests and verifications 16.81 4.67 3.19 20.00 5.56

TOTAL VI CHAPTER 46.22 12.84 8.78 55.00 15.28

GRAND TOTAL 59,290.18 16,469.50 11,040.95 70,331.13 19,536.43

Among which C+M (1.2+1.3+2+4.1+4.2+5.1.1) 16,352.17 4,542.27 3,106.91 19,459.08 5,405.30

SC INTERGROUP ENGINEERING SRLEconomist,Ana VasileThe chapters including the constituent expenses of the general estimate of costs as well as the estimates for each object are presented in detail in Appendix 1.


3.b Scale of expenses correlated with the graphics of the investment accomplishmentIn order to describe the total value of the investment divided on activity categories which constitutes the proposed project, a chart was elaborated, which includes the scale of expenses correlated with the graphics of the Investment achievement, as it can be observed below:

Scale of expenses correlated with the graphics of the investment accomplishment

Crtnumber

Designation of activity

Monetary unit

TOTAL COST VAT

excluded I Year Thousands of RON/lmonth II Year Thousands of RON/lmonth III Year Thousands of RON/lmonth

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36

1

Administrative activities of the project

Thousands of RON

989.96

28.13

29.95

29.95

29.95

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

38.97

1.1

Project management

Thousands of RON

967.35

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

26.87

1.2

The training and the approval of DL for a consultants selection in order to prepare the DL’s for acquisitions of service/goods/works’ and consultancy for project management during the project implementation period

Thousands of RON

1.26

1.26 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

1.3

The consultant’s selection for the DL’s arrangement of service/goods/works’ acquisition and consultancy for project management during the project implementation period

Thousands of RON

9.24 0

3.08

3.08

3.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

1.4

DL’s training and approval of turnkey project of Imaging Module

Thousands of RON 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

1.5

DL’s training and approval for the equipments’and furniture acquisition

Thousands of RON 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

1.6

DL’s training and approval for the acquisition of improvement services

Thousands of RON

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

1.7

DL’s training and approval for the acquisition of advertising services

Thousands of RON 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

1.8

The selection of a financial auditor

Thousands of RON 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

1.9

The accomplishment of the financial audit

Thousands of RON

12.10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

12.101

2.a

Turnkey project of Imaging Module

Thousands of RON

15,740.66 0.00 0.00 0.00 0.00 0.00 1.22 1.22 1.22 1.22

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32 0.00 0.00 0.00 0.00 0.00 0.00

2.a1

The deployment of works’ auction procedure for Turnkey project of Imaging Module

Thousands of RON

4.87 0 0 0 0 0 1.22

1.22

1.22

1.22 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

2.a2

Constuction, improvement and equipment for turnkey building

Thousands of RON

15,735.79 0 0 0 0 0 0 0 0 0

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32

749.32 0 0 0 0 0 0

2.a3

The final reception and equipment for turnkey building

Thousands of RON 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


2.b

Existing spaces improvement

Thousands of RON

1,513.20 0 0 0 0 0 0 0

0.53

0.53

0.53

0.53

125.93

125.93

125.93

125.93

125.93

125.93

125.93

125.93

125.93

125.93

125.93

125.93 0 0 0 0 0 0 0 0 0 0 0 0 0

2.b1

The deployment of works’ auction procedure and the selection of the service provider

Thousands of RON

2.10 0 0 0 0 0 0 0 0.53

0.53

0.53

0.53 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

2.b2

The execution of the arrangement works

Thousands of RON

1,511.10 0 0 0 0 0 0 0 0 0 0 0

125.93

125.93

125.93

125.93

125.93

125.93

125.93

125.93

125.93

125.93

125.93

125.93 0 0 0 0 0 0 0 0 0 0 0 0 0

2.b3

The reception of works

Thousands of RON 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

3

TARGET Module Endowment

Thousands of RON

38,472.85 0.00 0.00 0.00 0 0 0 0 0 0 0 0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.42 0.42 0.42 0.42

3497.38

3497.38

3497.38

3497.38

3497.38

3497.38

3497.38

3497.38

3497.38

3497.38

3497.38 0.00

3.1

The deployment of goods’ auction procedure and the selection of supplier / goods’ supplier

Thousands of RON

1.68 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.42

0.42

0.42

0.42 0 0 0 0 0 0 0 0 0 0 0 0

3.2

The delivery of equipment and furniture

Thousands of RON

38,437.56 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

3,494.32

3,494.32

3,494.32

3,494.32

3,494.32

3,494.32

3,494.32

3,494.32

3,494.32

3,494.32

3,494.32 0

3.3

Assembly, testing and reception

Thousands of RON

11.76 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

1.07

1.07

1.07

1.07

1.07

1.07

1.07

1.07

1.07

1.07

1.07 0

3.4

The training of personnel in order to use the new infrastructure

Thousands of RON

21.85 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.99

1.99

1.99

1.99

1.99

1.99

1.99

1.99

1.99

1.99

1.99 0

4

Acquisition of asstes for administration capacity einforcement

Thousands of RON

222.67 0 0 0.63

0.63

55.35

55.35

55.35

55.35 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

4.1

The deployment of goods’ auction procedure and the selection of supplier / goods’ supplier

Thousands of RON

1.26 0 0 0.63

0.63 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

4.2

The delivery of good’s

Thousands of RON

208.80 0 0 0 0 52.2 52.2 52.2 52.2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

4.3

Assembly, testing and reception

Thousands of RON

5.04 0 0 0 0

1.26

1.26

1.26

1.26 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

4.4

The training of personnel in order to use the new infrastructure

Thousands of RON

7.56 0 0 0 0 1.89

1.89

1.89

1.89 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

5Advertising

Thousands of RON

97.93 0 0

1.6807

- 0

30.961

30.961

30.961 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

1.68

1.68

- 0

5.1

The selection of advertising services’ supplier

Thousands of RON

1.68 0 0

1.68 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

5.2

The accomplishment and application of marks on the acquired equipments

Thousands of RON

3.36 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.68

1.68 0 0

5.3Audio-video

Thousands of RON

82.80 0 0 0 0 0 27.6 27.6 27.6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

5.4

Manufacture of posters and brochures

Thousands of RON

10.08 0 0 0 0 0

3.36

3.36

3.36 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Miscellaneous contingencies

Thousands of RON

2,252.92

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

62.58

GRAND TOTAL VAT excluded

Thousands of RON

59,290.18

90.71

92.53

94.84

93.16

144.80

176.98

176.98

177.51

91.20

839.30

839.30

964.70

964.70

964.70

964.70

964.70

964.70

964.70

964.70

964.70

965.12

965.12

965.12

839.20

4336.15

4336.15

4336.15

4336.15

4336.15

4336.15

3586.83

3586.83

3588.51

3588.51

3586.83

101.55


Further on, the investment major constituents are described in the adjacent charts:The cost of the investment major constituents VAT excluded

Monetary Unit I YEAR II YEAR III YEAR TOTAL

TARGET ConstructionThousands of RON/annually

2,042.73

8,170.93

4,085.47 14,299.14

Module arrangementsThousands of RON/annually

125.93

1,385.18

- 1,511.10

New equipmentThousands of RON/annually

-

-

36,502.20 36,502.20

EndowmentsThousands of RON/annually

190.80

-

1,935.36 2,126.16

Total Thousands of RON/annually

2,359.46

9,556.11

42,523.03 54,438.60

The cost of the investment major constituents VAT excluded Monetary Unit I YEAR II YEAR III YEAR TOTAL

TARGET ConstructionThousands of

EURO/annually 567.43

2,269.70

1,134.85

3,971.98

Module arrangementsThousands of

EURO/annually 34.98

384.77

-

419.75

New equipmentThousands of

EURO/annually -

-

10,139.50

10,139.50

EndowmentsThousands of

EURO/annually 53.00

-

537.60

590.60

Total Thousands of

EURO/annually 655.41

2,654.47

11,811.95

15,121.83


4 THE COST BENEFIT SURVEY

4.a Identification of investment and defining the objectivesThe total cost of investment for “CENTRE FOR TREATMENT AND RESEARCH IN GASTROENTEROLOGY BASED ON IMAGING METHODS AND MOLECULAR TECHNIQUES” includes expenses appendant to the building execution, laboratory’s arrangement and to the provision of equipment necessary for the research – development infrastructure.

The activity of construction and endowment of turnkey project of Imaging Building will be effected through 21 calendar months and the arrangements for the existing spaces will be effected through 12 calendar months.

Further on, the main elements of the capital costs appendant to the investment are presented in the chart below:

Capital constituentsValue

Thousands of RON VAT

excluded

ValueThousands of

EURO VAT excluded

TARGET Construction 14,299

3,971.98

Module arrangements 1,511

419.75

Equipment acquisition 36,502

10,139.50

Acquisition of IT equipments 1,386.36

385.10

Software application purchase 227.70

63.25

Furniture acquisition 321.30

89.25

Endowments acquisition for reinforcement of administration capacity

190.80

53.00

TOTAL 54,438.60

15,121.83

In order to have a general image of the viability of the investments’ project it is necessary to visualize in advance the evolution of the entries and exits appending to this on a long - and medium term. Thus, considering the nature of the infrastructure project, a time horizon has been taken into account for a 15 year analysis period.

With regard to the reference period, the year 2007 is considered the project’s reference year and the economic – financial analysis has as reference point the year 2007.


4.b Analyzing the options

Within this subchapter an analysis of the possible options for the present investment’s project will be made and the conclusion will be drawn by means of specifying the selected alternative.

For the investment project called “TARGET- CENTRE FOR TREATMENT AND RESEARCH IN GASTROENTEROLOGY BASED ON IMAGING METHODS AND MOLECULAR TECHNIQUES” there were 3 alternatives taken into consideration:

1. zero alternative (variant without investment)

2. medium alternative (variant with medium investment)

3. maximum alternative (variant with maximum investment)

Within the scope of fulfilling the objective of the proposed project, the zero alternatives or the variant without investment represents that option in which the existing spaces and endowments of the University of Medicine and Pharmacy of Craiova, there will be no investment of renewal, improvement or creation of research or equipment acquisition spaces.

The actual infrastructure (sesmic network) is 20 years old and it made up by equipments that are physically and morally overwhelmed. Under these circumstances, the national seismic system has difficulties in competing with other seismic networks and it cannot be used in implementing the project’s aims.

The medium alternative or the medium investment alternative could be the partial rehabilitation of equipment repairing or acquisition of certain equipment, as well as temporary arrangements of the space dedicated to the research – development.But not even this option provides the fulfillment of the objective of the investment project, because the infrastructure which will partially be improved can not develop the proposed research themes

The maximum alternative or the maximum investment variant suggests the arrangement of the research spaces as well as the acquisition of an infrastructure which, at present, does not exist in our country.

To conclude with, the selected variant consequently to the analyzing of alternatives is that of the maximum investment.


4.c Financial analysisThe financial analysis for the proposed investment project has been elaborated on the basis of The Guide for the Cost – Benefit Analysis and on the basis of the investment projects (The European Fund for the Regional Development, the Cohesion Fund and ISPA) and on the basis of the frame document no. 4 for „Guidance on the Methodology for Carrying out Cost Benefit Analysis”.

The financial analysis has as scope the use of the previsions of the project’s cash flow in order to determine the financial performance indicators such as: the cumulated flow, the investment or capital internal earning capacity rate and the corresponding updated net value

Taking into account that the proposed project does not have direct quantifiable incomes, a financial analysis is useful only for the cash flow evaluation. On the other hand, financial terms such as earning capacity, cost – benefit rate, updated net value are not applicable for the research infrastructure projects.

Thus, the obtained financial analysis for the given project is made up of a series of charts that provide information in regard to the detailing of financial data of the capital investment divided on categories of activities, to the costs and incomes corresponding to the working period, to the funding sources, to the cash flow analysis for the financial sustainability of the project.

Operation costs The operation costs have been established on the basis of the costs registered by the institution over the last years; the costs are estimated for the entire 15 year analysis period.

The operation costs represent annual expenses with materials used within the research process, wage costs for the personnel involved in the research activity, administrative expenses as well as expenses related to the maintenance and repairing of the research - development infrastructure.Also, an operation cost includes the cost regarding the main activity of the institute, as well as the cost of additional activities.

Wage expenses include the costs corresponding top the gross salaries of the employees and other expenses with the personnel. The wage expenses were based on the wage funds, on the basis of which the direct wage costs are calculated for the funding contracts closed starting with May 2007 out of budgetary funds allocated to the II National Plan, as they have been settled within the Appendix 3 of the Governmental Resolution no. 475 from 23rd of May 2007.

The administrative costs are detailed in costs regarding utilities (energy, water, phone, etc).

Within each category of expense it was considered the development of prices for the whole period, estimated in compliance with the development of the GDP (gross domestic product) forecast, made by the National Board of Forecast for the next 10 years, forecasting 5% annual increasing.

The costs’ development is described in the Appendix 2.

Operation incomes The results of the research activities carried out using the new created infrastructure for this project will generate long –and medium term incomes by participating in projects for the development of new systems, incomes which will represent financial sources for the deployment and development of other research activities.

The values of the projects and services drawn consequently to the TARGET implementation has been estimated depending on the participation both in the national and international using the new created infrastructure for tackling new problems regarding malignant digestive pathology.


To be mentioned are also the benefits obtained by means of participating in national and international projects, which represent an income source for which it was predicted a 5 % annual increase.

Through TARGET implementation will also be obtained incomes from economic activities namely will be fulfilled contracts with hospitals and banks where through will be applied the project results.

Thus, the obtained incomes will be used in the activities’ development within the domain malignant digestive pathology.

The incomes’ development is presented in Appendix 2.

4.d Economical analysisIn compliance with the Governmental Resolution no. 28 / 2008 effective starting with 23rd of February 2008, the economical analysis is compulsory only in case of major public investments.

The defining of major public investment presented in the Governmental Resolution no. 28 / 2008 describes the term as being that specific public investment whose total cost exceeds the equivalent of 25 Million Euro, in case of investments promoted within the domain of environmental protection, or the equivalent of 50 million Euro in case of investments promoted within other domains.

To conclude with, as a result of those previously mentioned, for the proposed project there is no need to elaborate an economic analysis.

4.e Sensibility analysisThe sensitivity analysis proposes to establish how much sensitive the objective future will be for some modifications of the key variables, that may occur throughout its future operation and it materializes in variations’ indicators in regard to the project’s financial and economical earning capacity – internal profitability ratios and the updated net revenue.

These indicators can not be calculated for the present project, because the research domain is not a domain that brings certain measurable incomes, and the drawn funding sources are meant to sustain the research activity and not to bring profit..

As a consequence for the previous stated matters, it is not the case to make a sensitivity analysis.

4.f Analysis of riskWithin this chapter the risks that may occur throughout the project’s deployment of implementation as well as the measures that may be applicable to their reduction have been presented.

The financial and technical risks of the project are presented in the adjacent matrix:

Current number

Type of risk Description Measures of reduction Evaluation

1 FinancialThe investment costs will be larger than the allocated budget

The continuous supervising of the market prices The adjustment of investment The drawing of complementary funds

Reduced

2 Financial The ulterior operation costs will not be sustained

Identification of new funding sources/partners Reduced

3 Technical The indicated technical specifications will correspond to some equipments morally

The continuous supervising of the technological progress The modification of the

Reduced


used at the moment of the acquisition deployment minimum specifications

4 Technical The acquired equipments are incompatible

The elaboration of technical specifications and auction documentations will aim at the compatibility of all equipments included in the project

Reduced

Within the auctions organized for the acquisition of equipment the following risks can be identified:

The obligation of repeating the acquisition procedures due to the reduced number of offers received accordingly – which will have a bad influence on the project’s action plan;

The non-compliance with the terms established for the handing over of equipment - due to some reasons which depend or not on the performer.

Internal risks:This category of risks depends directly on the way of activities’ deployment foreseen in the project’s action plan:

a) Errors in the installing of the acquired equipment;b) The poor planning of activities coming from to the management of project team;c) Difficulties in the training of personnel specialized in using the new equipments.

Internal risks at the delivery phase:d) The wrong phasing of the delivery period; e) The non-compliance with the regulation and effective legislation; f) Poor communication between the entities involved in the project’s implementation and the

equipment suppliers.

The management of project’s internal risks:

a) Margins for error have been foreseen in the logical and chronological planning of activities included in the action plan, for the more important stages of the project.

b) The checking stage of the new equipments’ installing will be emphasized;c) The Project Manager will directly take care of the collaboration in good terms with the

parties involved in the fulfillment of project;d) The compliance with the technical specifications will be pursued in regard to the acquired

equipment; e) The project’s classification according to the quality standards and to the foreseen terms will

be pursued; f) The personnel training in charge with the use of equipment will be requested from the

equipment suppliers.

Contractual riskWithin the scope of the reduction of contractual risk there will be strictly rules imposed in order to ensure the suppliers’ delivery capacity on time. Thus, there will be contracts as accurate as possible closed with the suppliers.


5 THE INVESTMENT FUNDING SOURCESThe funding for the non-repayable value of the public assistance corresponding to the project will be 100 % accomplished using the POS – CCE / CDI program, namely:

- 76.7% community financing - 23.3% national public financing (state budget).

Funding sources

Total investment cost (including VAT) Thousands of RON 70,331.13

Eligible costs Thousands of RON 59,290.18

Budget funding program POS-CCE/CDI % 100%(76,7%+23,3%)

Non-eligible costs Thousands of RON 0

Beneficiary financing % 100%

Non-eligible costs correspondent to VAT Thousands of RON 11,040.95

Financing from the main credit accountant in whose coordination / subordination is the beneficiary % 100%

According to the Solicitor’s Guide for the Development of the existent Public Infrastructure for the Research – Development and the building of a new infrastructure, the VAT will be supported from public funds by means of credit accountants’ budgets in whose coordination / subordination is the beneficiary.Considering the project’s nature and the perspective to gain incomes from research service rendering to the third party (impact evaluation surveys and environmental balances, technical –scientific assistance, physical - chemical analysis, biological tests) for income generating projects from such activities the financing deficit must be calculated.Thus, in compliance with the calculation methodology of the interference rate imposed by Working Document 4 for “Guidance on the Methodology for Carrying out Cost Benefit Analysis” the co-financing rate of the proposed project has been calculated.

Because the annual incomes obtained from third parties are not able to cover the annual expenses for the operation of laboratory, which will be arranged and endowed with equipment, the 15 year period updated net benefit is negative, which leads to a 100 % co-financing rate.The calculation method of the non-repayable assistance in case of the TENUME income generating project is presented in Appendix 3.


6 ESTIMATES RELATED TO THE OCCUPIED WORKING FORCE BY MEANS OF INVESTMENT ACCOMPLISHMENT

6.a Number of working places created in the execution phaseWithin the project’s execution period will be created five new jobs afferent to the project management team.

6.b Number of working places created in the operation phase

The estimated necessary work places after the project implementation is 49, as can be see as follow: - Digestive Imaging Module: Submodule Radiology and Imaging: PET-CT: 2+2 researchers / doctors (2 / shift, means 1 imaging techniques and 1 nuclear medicine); 2 operators, 1 physicist; 1 chemist; 2 nurses RM: 2 researcher / doctors (1 / shift); 2 operators; 1 nurse; 1 physicist Rx digital: 1 researcher / doctor; 1 nurse Digestive Endoscopy Submodule: 3 researcher / doctors, 1 operator, 6 nurses Endoscopic Surgery Submodule: 2 researcher / doctors, 1 operator, 1 technician, 2 nurses- Pathology and Immunology Module: 2 researchers / doctors; 1 operators; 2 nurses- Molecular Biology and Biochemistry Module: 3 researchers / doctors; 1 biochemist; 1 operators; 2 nurses- Telemedicine and Artificial Intelligence Module: 2 IT engineers; 1 technician


7 INVESTMENT MAIN TECHNICAL-ECONOMICAL INDICATORSWithin this chapter the investment’s technical – economical indicators will be detailed such as:The investment’s total value staged in months and years of the contract’s performance, the project’s execution period expressed in months, capacities presented in physical and value unities and other indicators specific for the domain of activity in which the investment is made, depending on each case.

Total value of the investment The total value of the investment staged in months of the contract’s performance is described in the chart below:


The investment staging The total investment’s staging in years of accomplishment of the investment project is presented in the chart below:

Current number

Stages in the investment accomplishment

Monetary Unit / year I Year II Year III Year

TOTAL COST

includingVAT

1Administrative activities of the project

Thousands of RON

994.25

1.1 Project management

Thousands of RON / year

322.45

322.45

322.45

967.35

1.2

The training and the approval of DL for a consultants selection in order to prepare the DL’s for consultancy during the project implementation period


1.50 0 0

1.50

1.3Preparation of the auction documentations


11.00 0 0

11.00

1.4 Financial audit

Thousands of RON / year 0 0

14.40

14.40

13.80

2Organization of the public acquisition procedures


11.80

2.00 -

13.80

3 Planning phase


66,451.16

3.3 Main investment


3,046.22

12,325.61

51,079.32

66,451.16

3,3,1 TARGET construction


2,669.31

10,677.26

5,338.63

18,685.20

3,3,2 Module arrangements


149.85

1,648.36 0

1,798.21

3,3,3 Equipment assembling


56.55

56.55

3,3,4 New equipments


43,381.07

43,381.07

3,3,5 Endowments


2,032.12

2,032.12

3,3,6 External assets


270.96

270.96

3.4Endowments for reinforcement of administration capacity


227.05 0 -

227.05

4 Project advertising

Thousands of RON / year 131.952 0

4.00

135.95

5 Miscellaneous and contingencies


893.66

893.66

893.66

2,680.97

6 Commissioning


40.00

55.00

GRAND TOTAL including VATThousands

of RON 4,433.58

13,543.72

52,353.83

70,331.13

Among which C+M: Thousands


of RON 19,459.08

Implementation periodThe implementation period of the investment project called „TARGET - CENTRE FOR TREATMENT AND RESEARCH IN GASTROENTEROLOGY BASED ON IMAGING METHODS AND MOLECULAR TECHNIQUES” amounts to 24 months. The duration of the project’s major activities is adjacent described:

Turnkey construction of IMAGING DEPARTMENT Building will be performed through 21 month calendar period;

The existing spaces arrangement will be performed through 12 month calendar period; The equipments’ acquisition of the project will be performed through 11 month calendar

period.

Capacities (in physical value units)The value units’ capacities represent those expectant social – economical benefits, the multiplier effect, and the physical units’ capacities are represented by infrastructure physical indicators.

In case of the evaluated project, both qualitative and quantitative indicators which fit into the above mentioned definitions will be presented.

Current number Value units’ capacities

1Training and working in common with students, postgraduates and young researchers for their specialization in research-development activities from digestive malignant pathology field will lead to the development of human resources from these sector.

2 Improving the general health level of the population by elaborating educational programs aiming at diminishing the risk of appearance of digestive affections.

3 The construction of TARGET Centre will allow to the achievement of significant progress that may have as a result the decrease of the morbidity and mortality rate.

4The TARGET Centre will offer the opportunity of endoscopic examinations. By the new infrastructure, the population will receive high level medical assistance in gastroenterology.

5 There will be used devices resulting in a better patient’s compliance due to the non-invasive procedures, and the development of new interventional methods as well.


Other particular indicators: o Special improvement – special improvements will be accomplished on a 438m2

surface (additional laboratories and capacities in the Molecular Biology and Biochemistry Module )

Studiu de fezabilitate – TARGET SF

INDICATORS Value at the beginning of the implementation

period

Value at the end of the implementation period

Achievement Indicators

New construction 0 1

Buildings improvement 0 2

New laboratories 0 15

Additional laboratories and capacities improved with regular facilities 0 19

Additional laboratories and capacities improved with special facilities (cleanroom) 0 11

RD equipments with the value of over 100.000 euro obtained by the project 0 15

Total number of equipments, including IT 0 314

Results Indicators

New jobs created in RD by the project (number) 0 20

Number of jobs maintained in RD due to the project 0 20

Number of young researchers 0 15

Number of women involved in research 0 8

Concerned research themes 0 4

International projects in which the infrastructure will be involved (number) 0 2

Under-privileged regions influenced by the project implementation 0 1

Transnational (regional) impact 0 da

Number of foreign researchers involved 0 3

The possibility of elaborating public policies in the health field 0 1

133

8 APROVALS AND AGREEMENTS

8.a Notice of investment beneficiary regarding the necessity and the opportunity of investment

You can see it in Appendix 4

8.b Urbanism certificateYou can see it in Appendix 5

8.c The principle approvals regarding the utilities availability (thermal and electric energy, methane gas, communications, water-sewerage etc)

In appendix 6, the charts with registration numbers for specific notices, requested in urbanism certificate.

8.d The environmental approvalIt’s not requested in city planning certificate.

8.e Other specific aprovals and principle agreementsAccording to the low 111/1996 (r2), Art. 8, Align. (7) the authorization of an achievement phase or nuclear/radiological devices functioning can be approved only if the anterior phases had all the necessary authorizations; and the Align. (8) according to art (7), the authorization phases of nuclear/radiological devices are, by the case, the following:a) Projection; b) Location; c) Production; d) Construction and/or montage; e) Commissioning of the equipments; f) Essay functioning; g) Exploitation; h) Repairing and/or maintenance; i) Changing; j) Preservation; k) Out of commission

In this condition, the acquisition of special advices will be done only after the achievement of infrastructure.


APPENDIX 11. Appendix 1. A.2. Appendix 1. B3. Appendix 1. C4. Appendix 1. D5. Appendix 1. E6. Appendix 1. F7. Appendix 1. G8. Appendix 1. H9. Appendix 1. I10.Appendix 1. J11.Appendix 1. K12.Appendix 1. L13.Appendix 1. M14.Appendix 1. N15.Appendix 1. O16.Appendix 1. P


Appendix 1 - General estimated of project in thousands euro and thousands lei.GENERAL ESTIMATE in compliance with the Government Resolution no. 28/2008 with reference to the

expenses necessary for the achievement of "TARGET – CENTRE OF TREATMENT AND RESEARCH IN GASTROENTEROLOGY BASED ON IMAGING METHODS AND MOLECULAR STUDIES" in thousands of LEI /

thousands of Euros Exchange rate 3.6 Lei/Euro

No.Chapters and

subchapters for investment expenses

Value (without VAT) TVA Value with VATThousands

leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

CHAPTER 1 Expenses for land acquisition and site preparation1.1 Land acquisition - - - - -

1.2 Land improvement 84.60 23.50 16.0

7 100.

67 27.9

7

1.3Environment protection works - - - - -

TOTAL CHAPTER 1: 84.60 23.50 16.0

7 100.

67 27.9

7

2 CHAPTER 2 Expenses for utilities provision

2.1 Costs for utilities 36.00 10.00 6.84 42.

84 11.9

0 TOTAL CHAPTER 2 36.00 10.00 6.84 42.84 11.9 CHAPTER 3: Expenses for design and technical assistance

3.1Topographical and geotechnical surveys 7.56 2.10 1.44

9.00

2.50

3.2Legal approvals for project implementation

166.53 46.26

31.64

198.17

55.05

3.3 Design and engineering 460.5

9 127.94 87.5

1 548.

10 152.2

5

3.4 Procurement (Tender Docs) 12.86 3.57 2.44 15.

30 4.2

5

3.5 Consultancy 1,102.9

4 306.37 25.7

6 1,128.

71 313.5

3

3.6 Technical assistance 13.22 3.67 2.51 15.

73 4.3

7

TOTAL CHAPTER 3 1,763.7

1 489.92 151.3

1 1,915.

01 531.9

5 CHAPTER 4 Investment expenses

4.1Construction and installations

15,810.24 4,391.73

3,003.94

18,814.18

5,226.16

4.2Set-up of technological equipment 47.52 13.20 9.03

56.55

15.71

4.3

Installations, technological and functional equipment with set-up

36,422.28 10,117.30

6,920.23

43,342.51

12,039.59

4.4

Installation without mounting and transport equipment 32.40 9.00 6.16

38.56

10.71

4.5 Endowments 1,898.4

6 527.35 360.7

1 2,259.

17 627.5

5

4.6 Intangible assets 227.7

0 63.25 43.2

6 270.

96 75.2

7


0 15,121.83 10,343.3

3 64,781.

93 17,994.

98


GENERAL ESTIMATE in compliance with the Government Resolution no. 28/2008 with reference to the expenses necessary for the achievement of "TARGET – CENTRE OF TREATMENT AND RESEARCH IN

GASTROENTEROLOGY BASED ON IMAGING METHODS AND MOLECULAR STUDIES" in thousands of LEI / Exchange rate 3.6 Lei/Euro

No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

CHAPTER 5: Other expenses

5.1 Site organization 455.57 126.55 86.56 542.13 150.595.1.1 Construction works 373.81 103.84 71.02 444.83 123.56

5.1.2Expenses related to site organization 81.76 22.71 15.53 97.30 27.03

5.2Fees, legal taxes and financial costs 212.58 59.05 0.00 212.58 59.05

5.2.1 Fees, legal taxes 212.58 59.05 0.00 212.58 59.05

5.2.1.1

Tax for National Inspectorate of Constructions ((0.7%+0.1%) x (C+M))

130.82 36.34 -

130.82

36.34

5.2.1.2

Contribution to the social constructors’ organization(0.5% x (C+M) 81.76 22.71 -

81.76

22.71

5.2.1.3Fees, agreements and authorizations (1% x (C+M)) - - - - -

5,2,2Credit cost (0.1% x (1.2+1.2+2+3+4+5.1+5.2.1)) - - - - -

5.3Contingencies (10% x (1.2+1.3+2+3+4))

2,252.92 625.81

428.05

2,680.97

744.71


6 811.41 514.6

1 3,435.

68 954.3

5 CHAPTER 6 Expenses for exploitation

6.1 Training 29.41 8.17 5.59 35.

00 9.7

2

6.2Technological tests, handing over expertise 16.81 4.67 3.19

20.00

5.56

TOTAL CHAPTER 6 46.22 12.84 8.78 55.

00 15.2

8

TOTAL GENERAL 59,290.1

8 16,469.50 11,040.9

5 70,331.

13 19,536.

43

C+M (1.2+1.3+2+4.1+4.2+5.1.1)

16,352.17 4,542.27

3,106.91

19,459.08

5,405.30


Appendix 1. AAPPENDIX 1 – OBJECT bill of quantities – IMAGING DEPARTMENT Building of UMF Craiova in

thousands lei/thousands euro Exchange rate 3.6 Lei/Euro

No. Chapters and subchapters for investment expenses


leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

I. MAIN WORKS AND INSTALLATIONS

1Earthworks 103.5

5 28.76 19.68 123.23 34.2

3

2Infrastructure 1,099.8

7 305.5

2 208.98 1,308.85 363.5

7

3Superstructure 3,740.8

8 1,039.1

3 710.77 4,451.65 1,236.5

7

4Architecture interior design 4,465.7

6 1,240.4

9 848.49 5,314.25 1,476.1

8

5Architecture exterior design 1,605.0

8 445.8

6 304.97 1,910.05 530.5

7

6Terraces construction 222.6

4 61.85 42.30 264.94 73.6

0

7Thermo installations 686.0

4 190.5

7 130.35 816.39 226.7

8

8Electrical installations 673.1

0 186.9

7 127.89 800.99 222.5

0

9Sanitary installation 569.5

5 158.2

1 108.21 677.76 188.2

7

10Air conditioning installations 750.7

6 208.5

5 142.65 893.41 248.1

7

11

Voice data equipment-fire advertisement-protection device

349.49 97.08 66.40 415.90

115.53

TOTAL I 14,266.7

4 3,962.9

8 2,710.68 16,977.41 4,715.9

5

II. MOUNTING

1Set-up of technological plants and equipment

10.80 3.00 2.05 12.85

3.57

TOTAL II 10.8

0 3.00 2.05 12.85 3.5

7

III. PROCUREMENT

1Technological and functional equipment 7.20 2.00 1.37 8.57

2.38

2 Transport equipment 6.48 1.80 1.23 7.71 2.1

4

3 Endowments 7.92 2.20 1.50 9.42 2.6

2

TOTAL III 21.6

0 6.00 4.10 25.70 7.1

4

TOTAL (TOTAL I + TOTAL II + TOTAL III)

14,299.14

3,971.98 2,716.84 17,015.97

4,726.66


Appendix 1.BAPPENDIX 1 – Object bill of quantities – Improvement of Digestive Imaging Module in thousands

lei/thousands euro

Exchange rate 3.6 Lei/Euro

No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7


1 Module improvement 204.8

0 56.8

9 38.91 243.72 67.70

TOTAL I 204.8

0 56.8

9 38.91 243.72 67.70

II. Set-up

1Set-up of technological plants and equipments - - - - -

TOTAL II - - - - -

III. PROCURAMENT

1Technological and functional equipment - - - - -

2 Transport equipment - - - - - 3 Endowments - - - - - TOTAL III - - - - -


204.80

56.89 38.91 243.72 67.70


Appendix 1.CAPPENDIX 1 - Object bill of quantities – Improvement of Pathology and Immunology Module in

thousands lei/thousands euro


No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7



0 70.0

0 47.88 299.88 83.30

TOTAL I 252.0

0 70.0

0 47.88 299.88 83.30

II. Set-up


TOTAL II - - - - -

III. PROCURAMENT




252.00

70.00 47.88 299.88 83.30


Appendix 1. D APPENDIX 1 - Object bill of quantities - Improvement of Molecular Biology and Biochemistry Module

in thousands lei/thousands euro


No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7



4 236.5

4 161.79 1,013.34 281.48

TOTAL I 851.5

4 236.5

4 161.79 1,013.34 281.48

II. Set-up


TOTAL II - - - - -

III. PROCURAMENT




851.54

236.54 161.79 1,013.34 281.48


Appendix 1.EAPPENDIX 1 - Object bill of quantities - Improvement of Telemedicine and Artificial Intelligence Module

in thousands lei/thousands euro


No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7



5 56.3

2 38.52 241.27 67.02

TOTAL I 202.7

5 56.3

2 38.52 241.27 67.02

II. Set-up


TOTAL II - - - - -

III. PROCURAMENT




202.75

56.32 38.52 241.27 67.02


Appendix 1.FAPPENDIX 1 - Object bill of quantities – Equipments acquisition for Digestive Imaging Module in


No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

I. 0 TOTAL I - - - - -

II. Set-up

1Set-up of technological plants and equipments

16.92

4.70 3.21 20.13 5.59

TOTAL II 16.9

2 4.7

0 3.21 20.13 5.59

III. PROCURAMENT

1Technological and functional equipment

27,709.20

7,697.00 5,264.75 32,973.95 9,159.43

1.1 PET-CT System 7927.20 2,202.0

0 1506.17 9433.37 2620.38

1.2 Cyclotron System 5148.00 1,430.0

0 978.12 6126.12 1701.70

1.3 Magnetic Resonance System (MRI) 8352.00

2,320.00 1586.88 9938.88 2760.80

1.4 Automat injector for contrast agent 117.00

32.50 22.23 139.23 38.68

1.5 Digital X-RAY System 1080.00 300.0

0 205.20 1285.20 357.00

1.6Autofluorescence gastroscope + colonoscope 378.00

105.00 71.82 449.82 124.95

1.7LIFS- optic biopsy forceps (life induced fluorescence) 313.20

87.00 59.51 372.71 103.53

1.8

High-resolution (HDTV) videoendoscopic system with NBI capabilities 352.80

98.00 67.03 419.83 116.62

1.9 Plasma argon coagulation system 83.52

23.20 15.87 99.39 27.61

1.103D magnetic positioning system for colonoscope 270.00

75.00 51.30 321.30 89.25

1.11

Radial and linear ultrasound endoscopy system, with contrast-enhanced EUS capabilities 549.00

152.50 104.31 653.31 181.48

1.12Dedicated ultrasound system with contrast-enhanced capabilities 597.60

166.00 113.54 711.14 197.54

1.13 Radial and linear ultrasound endoscope with elastography

548.28 152.30 104.17 652.45 181.24


APPENDIX 1 - Object bill of quantities – Equipments acquisition for Digestive Imaging Module in thousands lei/thousands euro


No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

capabilities

1.14

Dedicated ultrasound system with elastography and panoramic view capabilities 550.80

153.00 104.65 655.45 182.07

1.15Endoscopy and ultrasound endoscopy examination simulator 365.40

101.50 69.43 434.83 120.79

1.16

Dedicated endomicroscopy system (HD digital video processor + gastroscope + colonoscope), in completion of already acquired system 673.20 187.00 127.91 801.11 222.53

1.17

Endomicroscopy miniprobe system, compatible with the gastroscope, colonoscope and eneteroscope 403.20 112.00 76.61 479.81 133.28

2

Installation without set-up and transport equipment - - - - -

3 Endowments 668.63

185.73 127.04 795.67 221.02

3.1 Furniture 108.83

30.23 20.68 129.51 35.97

3.2 Endowments IT 532.44

147.90 101.16 633.60 176.00

3,2,1 Color Printer 64.80 18.0

0 12.31 77.11 21.42

3,2,2 Dry Laser Printer x 2 86.40 24.0

0 16.42 102.82 28.56

3,2,3

Endobase - software and hardware for medical imaging integration x 4 259.20

72.00 49.25 308.45 85.68

3,2,4 PC Server 10.80 3.0

0 2.05 12.85 3.57

3,2,5 PC x 2 14.40 4.0

0 2.74 17.14 4.76

3,2,6 Display x 3 3.24 0.9

0 0.62 3.86 1.07

3,2,7 UPS x 2 10.80 3.0

0 2.05 12.85 3.57

3,2,8 Printer x 2 7.20 2.0

0 1.37 8.57 2.38

3,2,9 Printer 3.60 1.0

0 0.68 4.28 1.19

3,2,10 Scanner 3.60 1.0

0 0.68 4.28 1.19Studiu de fezabilitate – TARGET SF 144

APPENDIX 1 - Object bill of quantities – Equipments acquisition for Digestive Imaging Module in thousands lei/thousands euro


No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

3,2,11 Switch/router 7.20 2.0

0 1.37 8.57 2.38

3,2,12 Wireless gateway 3.60 1.0

0 0.68 4.28 1.193,2,14 Display/turner x 2 10.80 3.00 2.05 12.85 3.573,2,15 Switch audio/video 18.00 5.00 3.42 21.42 5.953,2,16 DVD x 2 7.20 2.00 1.37 8.57 2.383,2,17 Notebook x 2 14.40 4.00 2.74 17.14 4.763,2,18 PDA x 2 7.20 2.00 1.37 8.57 2.38

3.3 Software 27.36 7.60 5.20 32.56 9.043,3,1 MS Office x 9 9.72 2.70 1.85 11.57 3.213,3,2 Corel Draw 3.60 1.00 0.68 4.28 1.193,3,3 Adobe Photoshop 2.16 0.60 0.41 2.57 0.713,3,4 Adobe premiere 2.16 0.60 0.41 2.57 0.713,3,5 Antivirus x 9 1.62 0.45 0.31 1.93 0.543,3,6 Windows x 9 8.10 2.25 1.54 9.64 2.68

TOTAL III 28,377.

83 7,882.7

3 5,391.79 33,769.62 9,380.45


28,394.75

7,887.43 5,395.00 33,789.75 9,386.04


Anexa 1.GAPPENDIX 1 - Object bill of quantities - Equipments acquisition for Pathology and Immunology

Module in thousands lei/thousands euro Exchange rate 3.6 Lei/Euro

No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

I. 0 TOTAL I - - - - -

II. Set-up

1Set-up of technological plants and equipment

16.20

4.50 3.08 19.28 5.36

TOTAL II 16.20

4.50 3.08 19.28 5.36

III. PROCUREMENT

1

Installations, technological and functional equipment with set-up

2,331.00

647.50 442.89 2,773.89 770.53

1.1 Manual rotational microtome 23.40 6.50 4.45 27.85 7.74

1.2 Cryostat 82.80 23.00 15.73 98.53 27.37

1.3 Immunohistochemistry automatic stainer 180.00 50.00 34.20 214.20 59.50

1.4Microscopy system with halogen and UV illumination 216.00 60.00 41.04 257.04 71.40

1.5 Laser microdissection system 612.00 170.00 116.28 728.28 202.30

1.6 Flow cytometry automated analyzer 1044.00 290.00 198.36 1242.36 345.10

1.7 Automated ELISA processor 172.80 48.00 32.83 205.63 57.12

2

Installation without mounting and transport equipment - - - - -

3 Endowments 271.26

75.35 51.54 322.80 89.67

3.1 Furniture 131.58 36.55 25.00 156.58 43.49

3.2 Endowments IT 122.04 33.90 23.19 145.23 40.34

3,2,1 PC Server 10.80 3.0

0 2.05 12.85 3.57

3,2,2 PC x 2 14.40 4.0

0 2.74 17.14 4.76

3,2,3 Display x 3 3.24 0.9

0 0.62 3.86 1.07

3,2,4 UPS x 2 10.80 3.0

0 2.05 12.85 3.57

3,2,5 Printer x 2 7.20 2.0

0 1.37 8.57 2.38

3,2,6 Printer 3.60 1.0

0 0.68 4.28 1.19


APPENDIX 1 - Object bill of quantities - Equipments acquisition for Pathology and Immunology Module in thousands lei/thousands euro


No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

3,2,7 Scanner 3.60 1.00 0.68 4.28 1.19

3,2,8 Switch/router 7.20 2.00 1.37 8.57 2.38

3,2,9 Wireless gateway 3.60 1.00 0.68 4.28 1.19

3,2,11 Monitor/tuner x 2 10.80 3.00 2.05 12.85 3.57

3,2,12 Switch audio/video 18.00 5.00 3.42 21.42 5.95

3,2,13 DVD x 2 7.20 2.00 1.37 8.57 2.38

3,2,14 Notebook x 2 14.40 4.00 2.74 17.14 4.763,2,15 PDA x 2 7.20 2.00 1.37 8.57 2.38

3.3 Software 17.64 4.90 3.35 20.99 5.833,3,1 Corel Draw 3.60 1.00 0.68 4.28 1.193,3,2 Adobe Photoshop 2.16 0.60 0.41 2.57 0.713,3,3 Adobe premiere 2.16 0.60 0.41 2.57 0.713,3,4 Antivirus x 9 1.62 0.45 0.31 1.93 0.543,3,5 Windows x 9 8.10 2.25 1.54 9.64 2.68

TOTAL III 2,602.2

6 722.8

5 494.43 3,096.69 860.19


2,618.46

727.35 497.51 3,115.97 865.55


Appendix 1.HAPPENDIX 1 - Object bill of quantities - Equipments acquisition Molecular Biology and Biochemistry in




leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

I. 0 TOTAL I - - - - -

II. Set-up

1Set-up of technological plants and equipments

14.40

4.00 2.74 17.14 4.76

TOTAL II 14.4

0 4.0

0 2.74 17.14 4.76

III. PROCUREMENT

1

Installations, technological and functional equipment with mounting

6,382.08

1,772.80 1,212.60 7,594.68 2,109.63

1.1Automated sample purification system coupled with Real-time PCR thermocycler 360.00 100.00 68.40 428.40 119.00

1.2 Microarray analysis platform864.00 240.00 164.16 1028.16 285.60

1.3Microfluidics-based platform for the analysis of DNA and RNA 180.00 50.00 34.20 214.20 59.50

1.4 Class III biohazard safety cabinet. Vertical airflow 46.80 13.00 8.89 55.69 15.47

1.5 Vacuum concentrator for DNA, RNA and proteins 28.80 8.00 5.47 34.27 9.52

1.6Complete system for karyotyping, FISH, MFISH, CGH and epi-fluorescence examination 252.00 70.00 47.88 299.88 83.30

1.7Automatic Denaturation/Hybridization System 32.40 9.00 6.16 38.56 10.71

1.8 Automatic Karyotyping System 72.00 20.00 13.68 85.68 23.80

1.9 VP 2000 Processor for slide 216.00 60.00 41.04 257.04 71.40 1.10 Flow Hood class II 36.00 10.00 6.84 42.84 11.90

1.11 ThermostatCell culture 18.00 5.00 3.42 21.42 5.95

1.12 Centrifuge 10.80 3.00 2.05 12.85 3.571.13 Heating Magnetic stirrer 2.52 0.70 0.48 3.00 0.83

1.14 Thermostatic water multiple baths 9.36 2.60 1.78 11.14 3.09

1.15 Incubator with CO2 23.40 6.50 4.45 27.85 7.74 1.16 Flow Hood class II 43.20 12.00 8.21 51.41 14.28

1.17 Refrigerated centrifuge with interchangeable-rotor 43.20 12.00 8.21 51.41 14.28

1.18 Laboratory microcentrifuge 36.00 10.00 6.84 42.84 11.90


APPENDIX 1 - Object bill of quantities - Equipments acquisition Molecular Biology and Biochemistry in thousands lei/thousands euro




leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

1.19Inverted fluorescence microscope for cell cultures examination with PC camera 90.00 25.00 17.10 107.10 29.75

1.20 Analytical Balance 18.00 5.00 3.42 21.42 5.951.21 2D electrophoresis system 57.60 16.00 10.94 68.54 19.04

1.22 MALDI -TOF-MS system 1080.00 300.00 205.20 1285.20 357.001.23 Homogenizing system 9.00 2.50 1.71 10.71 2.98

1.24 Luminescence spectrophotometer 324.00 90.00 61.56 385.56 107.10

1.25Chemiluminescence’s detection system for Western blots 108.00 30.00 20.52 128.52 35.70

1.26 AFM Microscope 900.00 250.00 171.00 1071.00 297.501.27 Refrigerator - 80°C 1.80 0.50 0.34 2.14 0.60

1.28 Autoclave 72.00 20.00 13.68 85.68 23.801.29 Drying oven 7.20 2.00 1.37 8.57 2.38

1.30 Two-photon laser scanning microscope 1440.00 400.00 273.60 1713.60 476.00

2

Installation without mounting and transport equipment

32.40

9.00 6.16 38.56 10.71

3 Endowments 234.97

65.27 44.64 279.62 77.67

3.1 Furniture 80.89 22.47 15.37 96.26 26.74

3.2 Endowments IT 136.44 37.90 25.92 162.36 45.10

3,2,1 Server PC 10.80 3.0

0 2.05 12.85 3.57

3,2,2 Work station PC x 2 14.40 4.0

0 2.74 17.14 4.76

3,2,3 Display x 3 3.24 0.9

0 0.62 3.86 1.07

3,2,4 UPS x 2 10.80 3.0

0 2.05 12.85 3.57

3,2,5 Printer x 2 7.20 2.0

0 1.37 8.57 2.38

3,2,6 Multifunctional printer 18.00 5.0

0 3.42 21.42 5.95

3,2,7 Scanner 3.60 1.0

0 0.68 4.28 1.19

3,2,8 Switch/router 7.20 2.0

0 1.37 8.57 2.38

3,2,9 Wireless gateway 3.60 1.0

0 0.68 4.28 1.19

3,2,11 Display/tuner x 2 10.80 3.0

0 2.05 12.85 3.57

3,2,12 Switch audio/video 18.00 5.0

0 3.42 21.42 5.95

3,2,13 DVD x 2 7.20 2.0

0 1.37 8.57 2.383,2,14 Notebook x 2 14.40 4.00 2.74 17.14 4.76


APPENDIX 1 - Object bill of quantities - Equipments acquisition Molecular Biology and Biochemistry in thousands lei/thousands euro




leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

3,2,15 PDA x 2 7.20 2.00 1.37 8.57 2.383.3 Software 17.64 4.90 3.35 20.99 5.83

3,3,1 Corel Draw 3.60 1.00 0.68 4.28 1.193,3,2 Adobe Photoshop 2.16 0.60 0.41 2.57 0.713,3,3 Adobe premiere 2.16 0.60 0.41 2.57 0.713,3,4 Antivirus x 9 1.62 0.45 0.31 1.93 0.543,3,5 Windows x 9 8.10 2.25 1.54 9.64 2.68

TOTAL III 6,649.4

5 1,847.0

7 1,263.40 7,912.85 2,198.01


6,663.85

1,851.07 1,266.13 7,929.98 2,202.77


Appendix 1.IAPPENDIX 1 - Object bill of quantities - Equipments acquisition Telemedicine and Artificial

Intelligence Module in thousands /thousands euro Exchange rate 3.6 Lei/Euro

No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

I. 0 TOTAL I - - - - -

II. Set-up

1Set-up of technological plants and machineries - - - - -

TOTAL II - - - - -

III. PROCUREMENT

1

Installations, technological and functional equipment with set-up - - - - -

2Installation and transport equipment - - - - -

3 Endowments 760.50

211.25 144.50 905.00 251.39

3.1 Furniture 0 0 0 0 0

3.2 Endowments IT 595.44

165.40 113.13 708.57 196.83

3,2,1 Server PC 10.80 3.0

0 2.05 12.85 3.57

3,2,2 DB server x2 72.00 20.0

0 13.68 85.68 23.80

3,2,3 Work stations x 6 43.20 12.0

0 8.21 51.41 14.28

3,2,4 Display x 7 12.60 3.5

0 2.39 14.99 4.17

3,2,5 Scanner 3D 7.20 2.0

0 1.37 8.57 2.38

3,2,8 Drivers + SDK kit 43.20 12.0

0 8.21 51.41 14.28

3,2,9

Server web/ftp/mail/network services 36.00

10.00 6.84 42.84 11.90

3,2,10 Router/gateway 36.00 10.0

0 6.84 42.84 11.90

3,2,11 Display x 4 7.20 2.0

0 1.37 8.57 2.38

3,2,12 UPS x 4 14.40 4.0

0 2.74 17.14 4.76

3,2,13 Printer x 2 7.20 2.0

0 1.37 8.57 2.38

3,2,14 Multifunctional printer x 2 14.40 4.0

0 2.74 17.14 4.76

3,2,15Integrated projecting system 10.80

3.00 2.05 12.85 3.57

3,2,16 Switch/router 7.20 2.0 1.37 8.57 2.38Studiu de fezabilitate – TARGET SF 151

APPENDIX 1 - Object bill of quantities - Equipments acquisition Telemedicine and Artificial Intelligence Module in thousands /thousands euro


No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

0

3,2,17 Wireless gateway x 2 7.20 2.0

0 1.37 8.57 2.38

3,2,18 Switch/Router 18.00 5.0

0 3.42 21.42 5.95

3,2,19Video-conference equipment 36.00

10.00 6.84 42.84 11.90

3,2,20 Display/turner 5.40 1.5

0 1.03 6.43 1.79

3,2,21 Switch audio/video 18.00 5.0

0 3.42 21.42 5.95

3,2,22 Video - projector 18.00 5.0

0 3.42 21.42 5.95

3,2,23 Video camera x 2 7.20 2.0

0 1.37 8.57 2.38

3,2,24 DVD 3.60 1.0

0 0.68 4.28 1.19

3,2,25 Notebook x3 21.60 6.0

0 4.10 25.70 7.14

3,2,26 Rack 10.80 3.0

0 2.05 12.85 3.57

3,2,27 PDA x 3 10.80 3.0

0 2.05 12.85 3.57

3,2,28 Access point x 2 14.40 4.0

0 2.74 17.14 4.76

3,2,29 Infrastructure 36.00 10.0

0 6.84 42.84 11.90

3,2,30 UPS x 2 21.60 6.0

0 4.10 25.70 7.14

3,2,32 Telephone Switchboard 44.64 12.4

0 8.48 53.12 14.763.3 Software 165.06 45.85 31.36 196.42 54.56

3,3,1 MS Office x 14 15.12 4.20 2.87 17.99 5.003,3,2 Corel Draw x 2 7.20 2.00 1.37 8.57 2.383,3,3 Adobe Photoshop x 2 7.20 2.00 1.37 8.57 2.383,3,4 Adobe premiere x 2 4.32 1.20 0.82 5.14 1.433,3,5 Antivirus for Server x 4 43.20 12.00 8.21 51.41 14.283,3,6 Windows for server x 4 14.40 4.00 2.74 17.14 4.763,3,7 SQL server x 4 14.40 4.00 2.74 17.14 4.763,3,8 Antivirus x 9 1.62 0.45 0.31 1.93 0.543,3,9 Microsoft C++ 7.20 2.00 1.37 8.57 2.38

3,3,10 SPSS 25.20 7.00 4.79 29.99 8.333,3,11 Statistics program 18.00 5.00 3.42 21.42 5.953,3,12 Visual Studio 7.20 2.00 1.37 8.57 2.38

TOTAL III 760.5

0 211.2

5 144.50 905.00 251.39


760.50

211.25 144.50 905.00 251.39


Appendix 1.JAPPENDIX 1 – Equipment acquisition table for the administrative capacity reinforcement in


No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

1 Endowments 291.60 81.00 55.40 347.00 96.39 1.1 Endowments IT 142.20 39.50 27.02 169.22 47.01

1,1,1 Server 36.00 10.00 6.84 42.84 11.901,1,2 Ups 18.00 5.00 3.42 21.42 5.951,1,3 Computer 16.20 4.50 3.08 19.28 5.361,1,4 Doc center 18.00 5.00 3.42 21.42 5.95

1,1,5Scanner system + plotter + soft 54.00 15.00 10.26 64.26 17.85

1.2 Software 48.60 13.50 9.23 57.83 16.071,2,1 Soft Primavera 36.00 10.00 6.84 42.84 11.901,2,2 External Mobilities X 2 12.60 3.50 2.39 14.99 4.17

1.3 Advertising 82.80 23.00 15.73 98.53 27.371,3,1 Website X 1 10.80 3 2.05 12.85 3.57

1,3,2 Advertising 72.00 20 13.68 85.68 23.80

1.4Access to data bases and publications 18.00 5.00 3.42 21.42 5.95

1,4,1 ISI Thompson - 2 14.40 4 2.74 17.14 4.761,4,2 Full text journals -10 3.60 1 0.68 4.28 1.19

TOTAL 291.60 81.00 55.40 347.00 96.39


Appendix 1.KAPPENDIX 1 - CHAPTER 1 - Expenses for land acquisition and site preparation

No.Chapters and



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

CAPITOLUL 1 Expenses for land acquisition and site preparation

1.1 Land acquisition -

-

-

- -

1.2Land clearing, vegetal soil cleaning

84.60

23.50

16.07

100.67 27.97

1,2,1 Architecture exterior design 84.6

0 23.5

0 16.0

7 100.6

7 27.97

1.3Environment protection works - - - - -

Total chapter 1: 84.6

0 23.5

0 16.0

7 100.6

7 27.97


Appendix 1.LAPPENDIX 1 - CHAPTER 2 - Utilities services expense



leiThousands

euroThousands

leiThousands

leiThousands

euro

1 2 3 4 5 6 7

CHAPTER 2 Utilities services expense

1.1 Utilities services expense 36.

00 10.0

0 6.84 42.

84 11.9

0

Total chapter 1: 36.

00 10.0

0 6.84 42.

84 11.9

0

Exchange rate RON/EURO 3.6


Appendix 1. MAPPENDIX 1 - CHAPTER 3 - Expenses for design, technical assistance and publicity

No.Chapters and


Value (without VAT) VAT Value with VAT

Thousands lei

Thousands euro

Thousands lei

Thousands lei

Thousands euro

1 2 3 4 5 6 7

CHAPTER 3 Expenses for design, technical assistance and advertising3.1 Site surveys 7.56 2.10 1.44 9.00 2.50

3,1,1 Topographical surveys 3.18 0.88 0.60 3.78 1.05 3,1,2 Geotechnical surveys 4.39 1.22 0.83 5.22 1.45

3.2Legal approvals for project implementation 166.53 46.26 31.64 198.17 55.05

3,2,1 Construction permit 163.52 45.42 31.07 194.59 54.05 3,2,2 Legal permits 3.01 0.84 0.57 3.58 1.00

3.3 Design and engineerging 460.59 127.94 87.51 548.10 152.25

3,3,1Imagistic and Research Centre Building 460.59 127.94 87.51 548.10 152.25

3,3,1,1 Design PT+CS+DE 438.66 121.85 83.34 522.00 145.00 3,3,1,2 Design checking 21.93 6.09 4.17 26.10 7.25

3.4 Procurement (Tender Docs) 12.86 3.57 2.44 15.30 4.25

3,4,1Evaluation assistance of design tenders 1.68 0.47 0.32 2.00 0.56

3,4,2Evaluation assistance of works tenders 4.62 1.28 0.88 5.50 1.53

3,4,2,1Imagistic and Research

Centre Building 2.52 0.70 0.48 3.00 0.83 3,4,2,2 Module arrangements 2.10 0.58 0.40 2.50 0.69

3,4,3

Evaluation assistance of service tenders for site engineer 0.67 0.19 0.13 0.80 0.22

3,4,4Evaluation assistance of service tenders for publicity 1.68 0.47 0.32 2.00 0.56

3,4,5Evaluation assistance of products tenders 1.68 0.47 0.32 2.00 0.56

3,4,6

Evaluation assistance of consultancy services for project management 1.26 0.35 0.24 1.50 0.42

3,4,7

Evaluation assistance for tenders and administrative capacity reinforcement 1.26 0.35 0.24 1.50 0.42

3.5 Consultancy 1,102.94 306.37 25.76 1,128.71 313.53

3,5,1Tender documents elaboration 9.24 2.57 1.76 11.00 3.06

3,5,2Consultancy services for project management 967.35 268.71 0 967.35 268.71

3,5,3,1 Project manager 468.30 130.08 0 468.30 130.08

3,5,3,2Responsible with

acquisition 127.21 35.34 0 127.21 35.343,5,3,3 Financial expert 56.01 15.56 0 56.01 15.56

3,5,3,4Scientific responsible for

equipment acquisition 315.83 87.73 0 315.83 87.733,5,3 Financial audit 12.10 3.36 2.30 14.40 4.00

3,5,4Management services for project publicity 114.25 31.73 21.71 135.95 37.76

3,5,4,1 Booklet/posters elaboration 10.08 2.80 1.92 12.00 3.33 3,5,4,2 Audio-Video Spots 100.80 28.00 19.15 119.95 33.32 3,5,4,3 Labels for equipment 3.36 0.93 0.64 4.00 1.11 3,5,4,4 Link on web page of Institute - - - - -


APPENDIX 1 - CHAPTER 3 - Expenses for design, technical assistance and publicity

No.Chapters and


Value (without VAT) VAT Value with VAT

Thousands lei

Thousands euro

Thousands lei

Thousands lei

Thousands euro

1 2 3 4 5 6 73.6 Technical assistance 13.22 3.67 2.51 15.73 4.37

3,6,1

Technical assistance from designer during execution period 0.62 0.17 0.12 0.73 0.20

3,6,2Site engineer-Hall arrangement 12.61 3.50 2.39 15.00 4.17

Total chapter 3: 1,763.71 489.92 151.31 1,915.01 531.95



Appendix 1.N APPENDIX 1 – CHAPTER 4 – Investment expenses



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

CHAPTER 4 Expenses for land acquisition and site preparation

4.1 Constructions and installations 15,810.24 4,391.73 3,003.94 18,814.18 5,226.16

4,1,1IMAGING DEPARTMENT Building 14,299.14 3,971.98 2,716.84 17,015.97 4,726.66

4,1,2Pathology and Immunology Module arrangement 252.00 70.00 47.88 299.88 83.30

4,1,3Digestive Imaging Module arrangement 204.80 56.89 38.91 243.72 67.70

4,1,4

Molecular Biology and Biochemistry module arrangement 851.54 236.54 161.79 1,013.34 281.48

4,1,5

Telemedicine and Artificial Intelligence module arrangement 202.75 56.32 38.52 241.27 67.02

4.2Mounting technological equipment 47.52 13.20 9.03 56.55 15.71

4.3

Installations, technological and functional equipment with mounting 36,422.28 10,117.30 6,920.23 43,342.51 12,039.59

4.4Installation without mounting and transport equipment 32.40 9.00 6.16 38.56 10.71

4.5 Endowments 1,898.46 527.35 360.71 2,259.17 627.55 4.6 Intangible assets 227.70 63.25 43.26 270.96 75.27

Total chapter 4: 54,438.60 15,121.83 10,343.33 64,781.93 17,994.98



Appendix 1.OAPPENDIX 1 - CHAPTER 5 – Other expenses



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

CHAPTER 5 Other expenses

5.1 Site organization 455.57 126.55 86.56 542.13 150.59

5,1,1Construction works (2,5% x (C+M)) 373.81 103.84 71.02 444.83 123.56

5,1,1,1IMAGING DEPARTMENT Building 373.81 103.84 71.02 444.83 123.56

5,1,2Expenses related to site organization 81.76 22.71 15.53 97.30 27.03

5.2Commissions, legal taxes and financing costs 212.58 59.05 - 212.58 59.05

5,2,1 Commissions, legal taxes 212.58 59.05 - 212.58 59.05

5,2,1,1

Tax for National Inspectorate of Constructions ((0.7%+0.1%) x (C+M)) 130.82 36.34 - 130.82 36.34

5,2,1,2

Contribution of social constructors’ organisation (0.5% x (C+M)) 81.76 22.71 - 81.76 22.71

5,2,1,3Fees, approvals and

building license (1% x (C+M)) - - - - -

5,2,2Credit cost (0.1% x

(1.2+1.3+2+3+4+5.1+5.2.1)) - - - - -

5.3Contingencies (max.10% x (1.2+1.3+2+3+4)) 2,252.92 625.81 428.05 2,680.97 744.71

Total chapter 5: 2,921.06 811.41 514.61 3,435.68 954.35



Appendix 1.PAPPENDIX 1 - CHAPTER 6 – Commissioning expenses



leiThousands

euroThousands

leiThousands

leiThousands

euro1 2 3 4 5 6 7

CHAPTER 6 Commissioning expenses

6.1 Training of personnel 29.41 8.17 5.59 35.0

0 9.72

6,1,1Personnel training for equipment use 10.92 3.03 2.08

13.00 3.61

6,1,2Personnel training for software use 10.92 3.03 2.08

13.00 3.61

Personnel training for using the equipments regarding administrative capacity reinforcement 7.56 2.10 1.44

9.00 2.50

6.2 Technological tests 16.81 4.67 3.19 20.0

0 5.56

6,2,1Installation and testing of equipment 11.76 3.27 2.24

14.00 3.89

Installation and testing of equipment for administrative capacity reinforcement 5.04 1.40 0.96

6.00 1.67

Total chapter 6: 46.22 12.84 8.78 55.0

0 15.28



APPENDIX 2


APPENDIX 2 - PROJECT SUSTAINABILITY

Description UM Table no.1 Data used for financial analysis Analysis period Year 15 InvestmentThe investment’s total cost (VAT excluding) among which:

Thousands RON 59,290

Eligible costsThousands

RON 59,290.1

8

Non-eligible costsThousands

RON 0Non-eligible costs corresponding to VAT

Thousands RON

11,040.95

* VAT is a non-eligible expense The investment’s total cost (including VAT)


Funding sources


RON 59,290Budget funding POS-CCE/CDI program % 100%

Non-eligible costsThousands

RON 0Beneficiary contribution % 100%Non-eligible costs corresponding to VAT


Financing from the credit accountant in whose coordination / subordination is the beneficiary % 100% Duration of Life equipments Construction years 55Module years 25Equipments years 15Furniture years 10Endowments IT years 4Endowments regarding administrative capacity reinforcement years 5


THE PROJECT’S FINANCIAL ANALYSIS year 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Table no.1- TOTAL CAPITAL INVESTMENT UM

Site arrangementThousands RON/year

84.60

-

-

TARGET BuildingThousands RON/year

2,042.73

8,170.93

4,085.47

Module ArrangementsThousands RON/year

125.93

1,385.18

-

New equipmentsThousands RON/year

-

-

36,502.20

FurnitureThousands RON/year

-

-

321.30

Endowments IT and software applications

Thousands RON/year

-

-

1,614.06

Endowments of administrative capacity reinforcement

Thousands RON/year

190.80

-

-

Total of fixed assetsThousands RON/year

2,444.06

9,556.11

42,523.03

UtilitiesThousands RON/year

3.00

33.00

-

Site surveysThousands RON/year

7.56

-

-

Public acquisitions, authorizationThousands RON/year

166.53

-

-

Design and engineeringThousands RON/year

460.59

-

-

Procurement (Tender Docs)Thousands RON/year

20.42

1.68

-

Site organizationThousands RON/year

65.08

260.33

130.16

Commissions, legal taxes and financing costs

Thousands RON/year

212.58

-

-

Technical assistanceThousands RON/year

0.09

0.35

0.18

Site supervisionThousands RON/year

1.80

7.20

3.60

Personnel trainingThousands RON/year

7.56

-

21.85

Installation, testing and receptionThousands RON/year

5.04

-

11.76

Project managementThousands RON/year

322.45

322.45

322.45

PublicityThousands RON/year

114.25

-

-

Financial auditThousands RON/year

-

-

12.10

ContingenciesThousands RON/year

750.97

750.97

750.97

The total costs of investment VAT excluded

Thousands RON/year

4,581.98

10,932.09

43,776.11

Eligible costsThousands RON/year

4,581.98

10,932.09

43,776.11


Non-eligible costsThousands RON/year 0 0 0

Non-eligible costs corresponding to VAT

Thousands RON/year

768.92

2,015.83

8,256.19

Total costs of investment including VAT

Thousands RON/year

5,350.91

12,947.93

52,032.30

year 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Table no 2- MAINTENANCE AND OPERATION COSTS UM Salary costs Thousands

RON/year 5,296.3

2 5,561.1

4 5,839.1

9 6,131.1

5 6,437.7

1 6,759.6

0 7,097.5

8 7,452.4

5 7,825.0

8 8,216.3

3 8,627.1

5 9,058.5

0

Raw Materials costs Thousands RON/year

506.50

531.83

558.42

586.34

615.66 646.44

678.76

712.70

748.34

785.75

825.04

866.29

Administrative costs Thousands RON/year

748.39

785.81

825.10

866.35

909.67 955.15

1,002.91

1,053.06

1,105.71

1,161.00

1,219.05

1,280.00

Fix assets with short lifetime period replacement

Thousands RON/year

Maintenance costs – RD infrastructure

Thousands RON/year 0 0 0

69.63

73.12

76.77

80.61

84.64

88.87

93.32

97.98

102.88

108.02

113.43

119.10

TARGET Building Thousands RON/year

28.60

30.03

31.53

33.11

34.76

36.50

38.32

40.24

42.25

44.37

46.58

48.91

Module arrangements Thousands RON/year

4.53

4.76

5.00

5.25

5.51

5.79

6.08

6.38

6.70

7.03

7.38

7.75

Equipments Thousands RON/year

36.50

38.33

40.24

42.26

44.37

46.59

48.92

51.36

53.93

56.63

59.46

62.43

TOTAL OPERATION AND MAINTENANCE COSTS

Thousands RON/year 0 0 0

6,620.85

6,951.89

7,299.48

7,664.46

8,047.68

8,450.06

8,872.57

9,316.20

9,782.01

10,271.11

10,784.66

11,323.89

year 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Table no 3- DEPRECIATION OF INVESTMENT TARGET Building

Investment costs Thousands RON/year

2,042.73

8,170.93

4,085.47

Depreciation Thousands RON/year

-

-

-

259.98

259.98

259.98

259.98

259.98

259.98

259.98

259.98

259.98

259.98

259.98

259.98

Module arrangements


125.93

1,385.18

-


-

-

-

60.44

60.44

60.44

60.44

60.44

60.44

60.44

60.44

60.44

60.44

60.44

60.44

Equipments


-

-

36,502.20


-

-

-

2,433.48

2,433.48

2,433.48

2,433.48

2,433.48

2,433.48

2,433.48

2,433.48

2,433.48

2,433.48

2,433.48

2,433.48

Furniture


-

-

321.30

Depreciation Thousands 32. 32. 32. 32. 32. 32.1 32. 32. 32. 32. Studiu de fezabilitate – TARGET SF 164

RON/year - - - 13 13 13 13 13 3 13 13 13 13 - - Endowments IT


-

-

1,614.06


-

-

-

403.52

403.52

403.52

403.52

- -

-

-

-

-

-

-

Endowments regarding administrative capacity reinforcement


190.80

-

-


-

-

-

38.16

38.16

38.16

38.16

38.16 -

-

-

-

-

-

-

Total investment costs Thousands RON/year

2,359.46

9,556.11

42,523.03

-

-

-

-

- -

-

-

-

-

-

-

Cumulated costs value Thousands RON/year

2,359.46

11,915.57

54,438.60

54,438.60

54,438.60

54,438.60

54,438.60

54,438.60

54,438.60

54,438.60

54,438.60

54,438.60

54,438.60

54,438.60

54,438.60

Total depreciation Thousands RON/year

-

-

-

3,227.71

3,227.71

3,227.71

3,227.71

2,824.20

2,786.04

2,786.04

2,786.04

2,786.04

2,786.04

2,753.91

2,753.91

Cumulated depreciation Thousands RON/year

-

-

-

3,227.71

6,455.43

9,683.14

12,910.85

15,735.05

18,521.09

21,307.13

24,093.17

26,879.20

29,665.24

32,419.15

35,173.06

Residual value RON/year 19,265.5

4

year 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Table no 4 – Funding sources UM Non-repayable funding from the budget program

Thousands RON/year

4,581.98

10,932.09

43,776.11

Financing from the credit accountant’s budget in whose coordination / subordination the beneficiary is among which:

Thousands RON/year

768.92

2,015.83

8,256.19

Financing of non- eligible costs corresponding to VAT

Thousands RON/year

768.92

2,015.83

8,256.19

Financing from other sources Thousands RON/year

1,115.25

1,171.01

1,229.56

1,291.04

1,355.59

1,423.37

1,494.54

1,569.26

1,647.73

1,730.11

1,816.62

1,907.45

State budget incomesThousands RON/year

935.52

841.97

757.77

681.99

613.79

552.41

497.17

447.46

402.71

362.44

326.20

293.58

Incomes obtained from participating at national and international projects

Thousands RON/year

179.73

329.04

471.79

609.04

741.79

870.95

997.36

1,121.81

1,245.02

1,367.67

1,490.42

1,613.87

Incomes obtained from economical activities

Thousands RON/year

5,505.60

5,780.88

6,069.92

6,373.42

6,692.09

7,026.70

7,378.03

7,746.93

8,134.28

8,540.99

8,968.04

9,416.44

Ultrasound Endoscopy LaboratoryThousands RON/year

153.60

161.28

169.34

177.81

186.70

196.04

205.84

216.13

226.94

238.28

250.20

262.71

Laser Confocal Endomicroscopy Laboratory

Thousands RON/year

72.00

75.60

79.38

83.35

87.52

91.89

96.49

101.31

106.38

111.70

117.28

123.14

Autofluorescence endoscopy Thousands 76. 80. 84. 88. 93. 98.0 102.9 108.0 113.4 119.1 125.1 131.3Studiu de fezabilitate – TARGET SF 165

RON/year 80 64 67 91 35 2 2 7 7 4 0 5 Chromoendoscopy and NBI laboratory

Thousands RON/year

38.40

40.32

42.34

44.45

46.68

49.01

51.46

54.03

56.73

59.57

62.55

65.68

Genomics LaboratoryThousands RON/year

336.00

352.80

370.44

388.96

408.41

428.83

450.27

472.79

496.43

521.25

547.31

574.67

Proteomics LaboratoryThousands RON/year

307.20

322.56

338.69

355.62

373.40

392.07

411.68

432.26

453.87

476.57

500.40

525.42

Performed proceduresThousands RON/year

4,521.60

4,747.68

4,985.06

5,234.32

5,496.03

5,770.83

6,059.38

6,362.35

6,680.46

7,014.49

7,365.21

7,733.47

Radiology investigationsThousands RON/year

141.60

148.68

156.11

163.92

172.12

180.72

189.76

199.25

209.21

219.67

230.65

242.18

Computed Tomography investigations

Thousands RON/year

840.00

882.00

926.10

972.41

1,021.03

1,072.08

1,125.68

1,181.96

1,241.06

1,303.12

1,368.27

1,436.69

MRI investigationsThousands RON/year

660.00

693.00

727.65

764.03

802.23

842.35

884.46

928.69

975.12

1,023.88

1,075.07

1,128.82

PET CT investigationsThousands RON/year

2,880.00

3,024.00

3,175.20

3,333.96

3,500.66

3,675.69

3,859.48

4,052.45

4,255.07

4,467.83

4,691.22

4,925.78

Total financial sourcesThousands RON/year

5,350.91

12,947.93

52,032.30

6,620.85

6,951.89

7,299.48

7,664.46

8,047.68

8,450.06

8,872.57

9,316.20

9,782.01

10,271.11

10,784.66

11,323.89

year 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Table no 5 - FINANCIAL SUSTAINABILITY UM

Total financial resources Thousands RON/year 5,351 12,948 52,032 6,621 6,952 7,299 7,664 8,048 8,450 8,873 9,316 9,782 10,271 10,785 11,324

Total inputs Thousands RON/year 5,351 12,948 52,032 6,621 6,952 7,299 7,664 8,048 8,450 8,873 9,316 9,782 10,271 10,785 11,324

Total operation and maintenance costs

Thousands RON/year 0 0 0 6,621 6,952 7,299 7,664 8,048 8,450 8,873 9,316 9,782 10,271 10,785 11,324

Total investment costs Thousands RON/year 5,351 12,948 52,032 0 0 0 0 0 0 0 0 0 0 0 0

Total outputs Thousands RON/year 5,351 12,948 52,032 6,621 6,952 7,299 7,664 8,048 8,450 8,873 9,316 9,782 10,271 10,785 11,324

Thousands RON/year

Total cash flow Thousands RON/year 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Cumulated cash flow Thousands RON/year 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


APPENDIX 3


APPENDIX 3 THE CALCULATION METHOD OF THE NON- REPAYABLE ASSISTANCE IN CASE OF THE INCOME GENERATING PROJECTS

Description IMPLEMENTARE OPERATION

Year 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15UM

THE PROJECT”S TOTAL VALUE VAT EXCLUDED

Thousands of RON

4,581.98

10,932.09

43,776.11

The investment eligible valueThousands

of RON 4,581.98

10,932.09

43,776.11

The investment non-eligible valueThousands

of RON - -

Pro-ratio of eligible costs % 100.00%

Operational expensesThousands of RON /an 0 0 0

6,620.85

6,951.89

7,299.48

7,664.46

8,047.68

8,450.06

8,872.57

9,316.20

9,782.01

10,271.11

10,784.66

11,323.89

Operational incomesThousands of RON /an 0 0 0

5,505.60

5,780.88

6,069.92

6,373.42

6,692.09

7,026.70

7,378.03

7,746.93

8,134.28

8,540.99

8,968.04

9,416.44

Residual valueThousands

of RON 0 0 0 0 0 0 0 0 0 0 0 0 0 0 19,265.54

Annual net incomesThousands of RON /an -1,115.25 -1,171.01

-1,229.56

-1,291.04 -1,355.59 -1,423.37

-1,494.54

-1,569.26 -1,647.73 -1,730.11 -1,816.62 17,358.09

The update ratio % 5% The annual update factor

The investment updated valueThousands

of RON 52,094.97 Cumulated operational net updated income

Thousands of RON

- 1,743.14

VNOA pro-ratio with the eligible costThousands

of RON- 1,743.14


of RON 53,838.11

Non-payable assistance ratio % 100.00%

Non-payable assistance valueThousands

of RON 59,290.18

The beneficiary’s financing valueThousands

of RON 0


Feasibility study – TARGET SF 169