Experiment 14 biochem

8/9/2019 Experiment 14 biochem

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LEARNING ACTIVITYNo.14

Protein Digestion


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g

I.OBJECTIVES:

At the en o! the e"#eri$ent% thest&ents 're e"#e(te to )e'rn ho* to:

1. E"#)'in the #ro(ess o! igestion o!

#rotien in !oo.+. ,rite the (he$i(') e-&'tion ino)ein the igestion o! #rotein.

/. Gie the igestie !&n(tion o! #e#sin'n tr0#sin.

4. Des(rie the e2e(t o! #3 'n the#resen(e o! $er(&r0 s')t on the h0ro)0sis

o! #rotein.


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II.ASSIGNENT:1. Arr'nge the !o))o*ing str&(t&re

!ro$ $ost (o$#)e" to the )e'st5'$ino '(is% #o)0#e#ties% #rotein.

#rotein #o)0#e#ties

'$ino '(is


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+. Co$#'re the (ontr'st #e#sin 'n tr0#sin.,h't 're there igestie !&n(tions6

Di2eren(es#3: #e#sin is '(tie or sho*s $'"i$&$ '(tiit0't '(ii( #3 o! 1.7 *hi)e tr0#sin is '(tie 't 'n

'si( #3 o! 8.9So&r(e:Pe#sin is se(rete 0 the $&(o&s )iningo! the sto$&(h region *hi)e tr0#sin is se(rete0 the #'n(re's 'n he)#s in igestion o! !oo

in s$')) intestineoe o! '(tion:#e#sin $'in)0 he)#s inh0ro)0sis o! #roteins *hi)e tr0#sin re's the#e#tie ons on the ('ro"0)sie o! se)e(tie

'$ino '(is s&(h 's )0sine 'n 'rginine.


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Pe#sin:1. It is se(rete !ro$ the g'stri( g)'ns o!sto$'(h.

+. It is se(rete in in'(tie !or$ ('))e#e#sinogen./. It '(ts on #rotein 'n (onerts the$ into#roteoses 'n #e#tones.4. It ('t')0ses in '(ii( $ei&$.

Tr0#sin:1. It is se(rete !ro$ the e"o(rine #'n(re's.+. It is se(rete in in'(tie !or$ ('))etr0#sinogen./. It '(ts on #roteoses 'n #e#tones 'n

(onerts the$ into #o)0#e#ties.4. It ('t')0ses in ')')ine $ei&$.


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The $'in si$i)'rities is th't oth 're#rote'ses 'n oth 're se(rete in

'n in'(tie !or$ ;(it0% the0 e'(h h0ro)0( #e#tie ons.


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/. Gie the e2e(t o! #3 'n the #resen(e o!$er(&r0 s')t on h0ro)0sis o! #rotein6

#3: en


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III. DISCSSION:

The igestion o! #rotien s to'$ino '(is st'rts in thesto$'(h. There the en


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V.PROCEDURE:1.P)'(e ' er0 )itt)e #ie(e o! h'r@

oi)e egg *hite $ore or )ess th'ns'$e in si


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4. A 7 $L o! BASIC PANCREATIN SOLTION ;/.7$L P'n(re'tin 1.7 $L .F Soi&$ ('ron'te=to the /r test t&e.

7. A 7 $L isti))e *'ter to the 4th test t&e.

F. A 7 $L o! '(ii>e #e#sin $er(&ri(

(h)orie so)&tion to the 7th test t&e.

8. P)'(e the test t&e into ' *'r$ *'ter 'th;'o&t 4 egree Ce)si&s= 'n osere !or 1 ho&r.

Re(or 0o&r oser'tions eer0 1 $in@inter')on the 't' t')e e)o*.


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VI. DATA ANDRESLTS:TIE;$i

n=Observation

PEPSIN ACIDIIEDPEPSIN

BASICPANCREATI

N

DISTILLED,ATER

ACIDIIED PEPSIN 3g C)+

START S$'))si


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TIE;$in=

Oser'tion


BASICPANCREA

TIN

DISTILLED,ATER

ACIDIIEDPEPSIN 3g

C)+

/ Itshrins' )itt)e'n it

isso)e' )itt)e

Contin&eto isso)e

s)o*)0

It s)o*)0isso)e

It s*e))s ')itt)e

It shrins ')itt)e

4 itisso)e' )itt)e

Contin&eto isso)e

s)o*)0

S'$e'##e'r'n

(e ;/$ins.=

It e(o$esigger(o$#'re tothe#reio&s

oser'tion

S'$e si


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TIE;$in=

Oser'tion


BASICPANCRE

ATIN

DISTILLED,ATER

ACIDIIEDPEPSIN 3g

C)+

F;1 3r= itisso)e' )itt)e

Contin&eto isso)e

s)o*)0

Contin&eto

isso)es)o*)0

S'$e'##e'r'n(e;7$ins.=

Contin&e toisso)es)o*)0


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VII. Conclusion:

Based on the experiment e

pre!ormed" e there!ore concludethat most proteins aredecomposed to sin#le amino acids

in di#estion.Di#estion t$picall$ be#ins in thestomach hen pepsino#en is

converted to pepsin b$ the actiono! h$drochloric acid. %$drochloricacid is used !or brea&in# the

bonds beteen the proteins.


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In the di(erent test tubes eobserved that in:

)ube *: thic& solid hite mass"partiall$ di#ested)ube + , hite cloud$ li-uid but

have small portion o! solid"extensivel$ di#ested)ube , ver$ small hite

molecules settled don" ver$little di#estion)ube / , hite solid mass" not

di#ested


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)ube * is onl$ partiall$di#ested because it also

needs %Cl to completeprotein di#estion.

)ube + is di#ested becauseit is the ideal environmento! the stomach)ube shos littledi#estion because sodium

carbonate is used and acts


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)ube / is not di#estedbecause it needs pepsin to

turn protein into aminoacids

tube 0 shos littledi#estion because 1ercuricchloride.


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PO2) 34BOR4)OR56UE2)IO'2:


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C'n the o0 store ('roh0r'tes6 't6Protein6 I! so% in *h't !or$6

Yes% o&r o0 h's ' )i$ite stor'ge ('#'(it0!or ('roh0r'tes in the $&s()e g)0(ogen'n the )ier g)0(ogen. ,hen those s#)ies

're !&))% e"(ess ('roh0r'tes 're store 's!'t. The o0 stores ('roh0r'te 's#l$co#enin the $&s()es 'n )ier% ho*eerits stor'ge ('#'(it0 is )i$ite.

,hen 0o& (ons&$e $ore ('roh0r'testh'n 0o& i$$ei'te)0 nee% 0o&r o0stores the$ 's ' s&st'n(e ('))e

#l$co#en.


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The o0 oes not store #rotein% r'ther it

t'es *h't it nees !ro$ oth the '$ino'(i #oo) 'n *h't is erie !ro$ !ooso&r(es 'n &ses it !or the ne(ess'r0

!&n(tions $entione. ,hen $ore #roteinis (ons&$e th'n *h't the o0 nees%the #rotein is #ro(esse thro&gh the )ier%>)tere thro&gh the ine0 'n thene"(rete thro&gh &rine. or this re'son%Ber'ri re(o$$ens th't *o$en neeon)0 to (ons&$e + to / g o! #rotein 't '

ti$e% 'n !or $en% 4 to F g is s&?(ient.


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,hen 0o& e't $ore !'ts th'n 0o&i$$ei'te)0 nee% 0o&r o0 stores the$'s ' s&st'n(e ('))e tri#l$ceride" ortriac$l#l$cerol.

,hen 0o& e't $ore !'t th'n 0o& nee !or0o&r short@ter$ !&n(tion% !'tt0 '(is !ro$0o&r )oostre'$ (o$ine insie 0o&r !'t

(e))s *ith ' s&st'n(e ('))e #l$cerol'n !or$ tri#l$ceride. ,hen 0o& nee tor'* on 0o&r !'t reseres% trig)0(erie is

roen '#'rt 'n sent '( into 0o&r


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,h't is the nor$') 'i)0 re-&ire$ent o!#rotein6 ,h't ('n '2e(t this '$o&nt6

Protein is the essenti') n&trients !or theh&$'n o0. The0 're one o! the &i)ing)o(s o! o0 tiss&e% 'n ('n ')so sere

's ' !&e) so&r(e. As !&e)% #roteins (ont'in/ &cal per #ram% H&st)ie ('roh0r'tes 'n &n)ie )i#is%

*hi(h (ont'in (') #er gr'$. Proteins 're #o)0$er (h'ins $'e

o! '$ino '(is )ine together 0 #e#tie

ons.


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Protein is !o&n in the !o))o*ing !oos: $e'ts%

#o&)tr0% 'n >sh% )eg&$es ;r0 e'ns 'n #e's=%to!&% eggs% n&ts 'n sees% $i) 'n $i)#ro&(ts% gr'ins% so$e eget')es% 'n so$e!r&its ;#roie on)0 s$')) '$o&nts o! #rotein

re)'tie to other so&r(es=.,hen ' high iet'r0 #rotein int'e is (ons&$e%

there is 'n in(re'se in &re' e"(retion% *hi(hs&ggests th't '$ino '(i o"i'tion is in(re'se.n)ie !'t 'n g)&(ose% o&r o0 h's )itt)e('#'(it0 to store #rotein. I! *e *ere to sto#e'ting #rotein% o&r o0 *o&) st'rt to re'

o*n $&s()e !or its nees *ithin ' '0 or so.


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T3EEND

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Experiment 14 biochem