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7/28/2019 Examples of Bacterial Identification
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Identification of bacteria
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B
iochem
icaltestforbacteria
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Staining
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G
ramsstain
appearance interpret
Purple Gram positive
Pink to red Gram negative
Gram positiveGram negative
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Appearance on bacteriologicalagar
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She
epbloo
dplate
Beta-hemolysisAlpha-hemolysis
Gamma-hemolysis
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Ma
cConkeyagar
colony appearance interpret
1 pink colony Lactose fermenter
2 pink colony Lactose fermenter
3 no growth -
4 colorless colony Non lactose fermenter
7/28/2019 Examples of Bacterial Identification
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igella-Sa
lmonella
Agar(S
Sagar)
Colorless colony with black center
Pink colony
colony appearance interpret
1 pink colony Lactose fermenter
2 Colorless colony Non Lactose fermenter
3 Colorless colony with black center Non Lactose fermenter
H2S producing colony
Colorless colony
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V.parahaemolyticusand V.choleraon TCBS
TCBS
Green colony
Yellow colony
colony appearance interpret
1 Green colony Non sucrose fermenter
2 Yellow colony Sucrose fermenter
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biochemical test
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O2
O2
O2
T
ripleSu
garIronAgar(TSI)
K/ADelayed reaction
No
fermentation
pH= 7.4
O2
Dextrose
Mixed acid
Dextrose
+ Lactose
Mixed acid
Dextrose
Mixed acid
A/AInitial reaction
A/A
K/N
Non fermenter
Non lactose fermenter
lactose fermenter
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ripleSu
garIronAgar(TSI)
tube 1 2 3 4
appearance Slant Yellow red red red
Butt Yellow yellow yellow neutral
Gas + + - -
H2S - - + -
interpret Slant/butt, gas,H
2S
A/AG K/AG K/A+ K/N
1 2 3 4
-Fermentation
Yellow ~Acid (A)
Red~Alkaline (K)
No change ~Neutral (N)
-Gas positive G
-H2S positive +
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LysineIron
Agar(LIA)
tube control 1 2 3 4appearance Slant purple purple red purple purple
Butt purple yellow yellow purple purple
H2S - - - +
interpret Slant/butt -/- +/- -/+ -/+
Slant Lysine Deaminase (LDA)
purple = negative
non LDA producerred=positive LDA producer
Butt Lysine Decorboxylase (LDC)
yellow =negative non LDC producerpurple = positive LDA producer
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SIM
tube 1 2 3
sulfur - - +
indole - + +
motile - + +
Sulfur, indole, motile -/-/- -/+/+ +/+/+
1 2 3
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Simmons Citrate agar
1 2
tube 1 2
appearance bacteria No growth growth
agar Not change Blue
interpret - +
- To tests the ability of organisms to utilize citrate as a carbon source
- Sodium citrate is the sole source of carbon
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Tolerance testing
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Bacitrac
insusce
ptibility
test
Clear zone
susceptibility positive
No clear zone resistance negative
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control 1 2
appearance clear clear turbid
interpret - Negative (-) Positive (+)
6.5
%N
aCl
1 2
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Immunological testing
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Anti-serum agglutination test
1 2
appearance Non agglutinate agglutinated
interpret Negative (-) Positive (+)
1 2