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Evolutionary Considerations in Sexual Reproduction More Rotifer Strangeness. Andrew J. Pierce. Microbiology, Immunology and Molecular Genetics Graduate Center for Toxicology Markey Cancer Center University of Kentucky. MI615. Reassembly of shattered chromosomes in Deinococcus radiodurans. - PowerPoint PPT Presentation
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Evolutionary Considerations in Sexual Reproduction
More Rotifer Strangeness
MI615
Andrew J. PierceMicrobiology, Immunology and Molecular Genetics
Graduate Center for ToxicologyMarkey Cancer CenterUniversity of Kentucky
Reassembly of shattered chromosomes in Deinococcus radiodurans.Nature. 2006 Oct 5;443(7111):569-73. Epub 2006 Sep 27.
Zahradka K, Slade D, Bailone A, Sommer S, Averbeck D, Petranovic M, Lindner AB, Radman M.
Reassembly of shattered chromosomes in Deinococcus radiodurans.Nature. 2006 Oct 5;443(7111):569-73. Epub 2006 Sep 27.
Zahradka K, Slade D, Bailone A, Sommer S, Averbeck D, Petranovic M, Lindner AB, Radman M.
Not Idigests Red: irradiated
7000 Gy γ-IR (!)
(hours post-IR)
Figure 1
Reas
sem
bly
of sh
atter
ed c
hrom
osom
es in
Dei
noco
ccus
radi
odur
ans.
Nat
ure.
200
6 O
ct 5
;443
(711
1):5
69-7
3. E
pub
2006
Sep
27.
Zah
radk
a K,
Sla
de D
, Bai
lone
A, S
omm
er S
, Ave
rbec
k D,
Pet
rano
vic
M, L
indn
er A
B, R
adm
an M
.
Reassembly of shattered chromosomes in Deinococcus radiodurans.Nature. 2006 Oct 5;443(7111):569-73. Epub 2006 Sep 27. Zahradka K, Slade D, Bailone A, Sommer S, Averbeck D, Petranovic M, Lindner AB, Radman M.
Figure 2
neg
IR
IR +
3h
IR +
3h +
UV
IR +
3h +
Brd
U
IR +
3h +
Brd
U +
UV
UV doseUV dose
Grown in BrdU Repaired in BrdU
Reas
sem
bly
of sh
atter
ed c
hrom
osom
es in
Dei
noco
ccus
radi
odur
ans.
Nat
ure.
200
6 O
ct 5
;443
(711
1):5
69-7
3. E
pub
2006
Sep
27.
Zah
radk
a K,
Sla
de D
, Bai
lone
A, S
omm
er S
, Ave
rbec
k D,
Pet
rano
vic
M, L
indn
er A
B, R
adm
an M
.
Reas
sem
bly
of sh
atter
ed c
hrom
osom
es in
Dei
noco
ccus
radi
odur
ans.
Nat
ure.
200
6 O
ct 5
;443
(711
1):5
69-7
3. E
pub
2006
Sep
27.
Zah
radk
a K,
Sla
de D
, Bai
lone
A, S
omm
er S
, Ave
rbec
k D,
Pet
rano
vic
M, L
indn
er A
B, R
adm
an M
.
Figure 3
NAT
IVE
DEN
ATU
RIN
GssDNA
dsDNA
Reas
sem
bly
of sh
atter
ed c
hrom
osom
es in
Dei
noco
ccus
radi
odur
ans.
Nat
ure.
200
6 O
ct 5
;443
(711
1):5
69-7
3. E
pub
2006
Sep
27.
Zah
radk
a K,
Sla
de D
, Bai
lone
A, S
omm
er S
, Ave
rbec
k D,
Pet
rano
vic
M, L
indn
er A
B, R
adm
an M
.
Figure 4
Extreme resistance of bdelloid rotifers to ionizing radiation.
Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5139-44. Epub 2008 Mar 24.Gladyshev E, Meselson M.
Fertility: producing at least one active daughterFecundity: percent hatched eggs
Dose: 140 Gy / hr
Mon
ogon
ant
Bdel
loid
Table 1
Extreme resistance of bdelloid rotifers to ionizing radiation.Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5139-44. Epub 2008 Mar 24. Gladyshev E, Meselson M.
Dose dependence of bdelloid and monogonont reproduction
ParentalReproduction
F1 Reproduction
Diamonds: DSB / MbpCircles: E. dilatata (monogonant)Squares: P. roseola (bdelloid)Triangles: A. vaga (bdelloid)
Fertility: empty symbolsFecundity: filled symbols
Figure 1
Extreme resistance of bdelloid rotifers to ionizing radiation.Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5139-44. Epub 2008 Mar 24. Gladyshev E, Meselson M.
DNA double-strand breakage by IR in A. vaga (bdelloid)
Extreme resistance of bdelloid rotifers to ionizing radiation.Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5139-44. Epub 2008 Mar 24. Gladyshev E, Meselson M.
Figure 2
Standard breakage: 5 x 10-3 breaks / Mbp / Gy
Massive horizontal gene transfer in bdelloid rotifers.
Science. 2008 May 30;320(5880):1210-3.Gladyshev EA, Meselson M, Arkhipova IR.
Massive horizontal gene transfer in bdelloid rotifers.Science. 2008 May 30;320(5880):1210-3. Gladyshev EA, Meselson M, Arkhipova IR.
Massive horizontal gene transfer in bdelloid rotifers.Science. 2008 May 30;320(5880):1210-3. Gladyshev EA, Meselson M, Arkhipova IR.
Mas
sive
horiz
onta
l gen
e tr
ansf
er in
bde
lloid
rotif
ers.
Scie
nce.
200
8 M
ay 3
0;32
0(58
80):1
210-
3. G
lady
shev
EA,
Mes
elso
n M
, Ark
hipo
va IR
.
Mas
sive
horiz
onta
l gen
e tr
ansf
er in
bde
lloid
rotif
ers.
Scie
nce.
200
8 M
ay 3
0;32
0(58
80):1
210-
3. G
lady
shev
EA,
Mes
elso
n M
, Ark
hipo
va IR
.
