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Bruker Eva Basics MIME Oregon State University
11/14/2014 Page 1 Austin Fox ([email protected]) Licensed CC BY 4.0
Eva Basics Table of Contents Opening Files and basic manipulation 1 Smoothing “Scans” 2 Removing Background 2 Fit Peaks 3
Find chord mid, FWHM, and integrated intensity 3 Peak Search (finding peaks for other analysis) 3
Smoothing and Background removal are required before this step 3 Search Match Peaks 4
Find potential matches to your pattern 4 Making New DIF 5
Subtracting matched peaks to match remaining 5 Labeling 5
If for some reason you want to use Eva to create images to use in a report 5 Opening Files and basic manipulation
1. Log into user account 2. Open up “Eva” (see fig. to right) 3. Open pattern and analyze
a. Important Basic Buttons are as follows: 1. New “.EVA” file 2. Import patterns for comparison 3. Open file (.EVA, .RAW) 4. Save “.EVA” file – saves all work 5. Print/print preview 6. Undo/redo/reset zoom 7. Multi file alignment 8. Help 9. ToolBox on/off 10. Search/Match on/off 11. Search Results on/off
Bruker Eva Basics MIME Oregon State University
11/14/2014 Page 2 Austin Fox ([email protected]) Licensed CC BY 4.0
Smoothing “Scans” 1. Select “Scan” to modify in the ToolBox 2. At the bottom of the ToolBox, Select “Fouier” or “Smooth” 3. Adjust settings until a desirable smoothness is reached
a. Do not increase levels too much or you will create erroneous data (exaggerated bellow)
4. Select “Replace” or “Append” depending on your preference
a. Append will create a new “Scan”
Removing Background 1. Select “Scan” to modify in the ToolBox 2. At the bottom of the ToolBox, Select “Backgrd” 3. Adjust settings until a desirable background is set to be removed
a. Enhanced removal can be useful to maintain peak breath and small peaks (below in red)
4. Select “Replace” or “Append” depending on your preference b. Append will create a new “Scan”
Bruker Eva Basics MIME Oregon State University
11/14/2014 Page 3 Austin Fox ([email protected]) Licensed CC BY 4.0
Fit Peaks Find chord mid, FWHM, and integrated intensity
1. Under “Area” in the ToolBox a. Click “Create” b. Click points at edges of peak c. Coordinates can be adjusted after initial selection
Peak Search (finding peaks for other analysis) Smoothing and background removal are required before this step
1. Complete Smoothing and Background removal 2. Select the corrected “Scan” to modify in the ToolBox 3. At the bottom of the ToolBox, Select “PeakSearch” 4. Adjust settings until all peaks are matched and there are no excess peaks
a. A combination of threshold and width will need to be used
5. Select “Make DIF” a. Adds peaks to pattern list
Bruker Eva Basics MIME Oregon State University
11/14/2014 Page 4 Austin Fox ([email protected]) Licensed CC BY 4.0
Search Match Peaks Find potential matches to your pattern
1. Turn on Search/Match and Search Results 2. Select the appropriate DIF “pattern” 3. In Search/Match
a. Toggle all elements off, aka turn them “red” b. “Grey” the elements that may be in your sample’s structure c. “Green” the elements that must be in the structure d. Adjust other settings as necessary e. Ensure the arrow beside “Pattern” is “Green” f. Click “Search”
4. In Search Results, click and drag possible results into ToolBox “Patterns” a. Select based on FOM and personal thoughts
5. Once good match is found and peaks still exist, try Making a New DIF (see below)
Bruker Eva Basics MIME Oregon State University
11/14/2014 Page 5 Austin Fox ([email protected]) Licensed CC BY 4.0
Making New DIF Subtracting matched peaks to match remaining peaks
1. Select DIF from which to edit peaks - “Pattern” in the ToolBox 2. Click “Append To List”
a. This creates peaks on “Peak” page of ToolBox 3. Determine which peaks to remove By using the check boxes 4. Select all remaining peaks using ctrl key 5. Click “Make DIF”
Labeling If for some reason you want to use Eva to create images to use in a report
1. Open “Label” page of ToolBox and play around
