ISO-compliant chromogenic medium

ESIA is the ISO/TS 22964 standard required medium for the detection of Cronobacter spp. in powdered milk and powdered infant formula

Additional reagents required according to this ISO method are available: ESIA Agar, Buffered Peptone Water, mLST broth, TSA agar

Easy-to-use: Easy-to-interpret results: blue-green colonies on a clear background

Convenient protocol “1 bag/1 plate”: ESSB broth formulated to provide optimal growth of Cronobacter spp. while inhibiting interfering flora on ESIA agar

Results in 21 ± 3 hours at 44 ± 1˚C

Chromogenic culture medium for the specific detection

of Cronobacter spp. (including Cronobacter sakazakii) in food samples such as milk powder, powdered infant

formula and food prepared from milk powder

Typical blue-green colonies: Cronobacter spp.

esiaTM

esiaTM

bioMérieux Industry595 Anglum Road

Hazelwood, MO 63042 • U.S.A.

Tel: (800) 634 7656

Fax: (314) 731 8678

bioMérieux Canada, Inc.7815 Henri-Bourassa Boulevard West

St. Laurent (Québec) • Canada H4S 1P7

Tel: (1) 514 336 7321

Fax: (1) 514 807 0015

www.biomerieux-usa.comDU

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> ESIA Medium

20 plates 90mm Ref. AEB520010

> ESIA Agar-Dehydrated

500g Ref. AEB150002

> Other media:

ESSB Broth - Cronobacter spp.6 x 225ml bottles Ref. AEB611448

Modified LST Broth100 x 10ml tubes Ref. AEB110549

Tryptic Soy Agar10 plates 90mm Ref. M1040

Buffered Peptone Water 500g Ref. 46925 100 x 9ml tubes Ref. 421116 x 90ml bottles Ref. 42042 6 x 225ml bottles Ref. 4204310 x 225ml Minibags Ref. 427293 x 3L bags Ref. 42629

Selective isolation agar for Cronobacter spp. (including C. sakazakii) detection in infant milk powders

Alternative method for the detection of Cronobacter spp. according to ESSB/ESIA protocol

Homogenize X g of sampleIn 9 X ml of ESSB Broth

Incubate for 24 ± 2 hours at 37 ± 1˚C

Isolate on ESIA agarIncubate for 21 ± 3 hours at 44 ± 1˚C

Presence of typical colonies (Blue colonies)

Confirmation with biochemicals or VITEK

automated identification system

Absence of typical colonies(Purple colonies)

Absence of Cronobacter spp. in X grams of sample