Share Our Advanced Scientific Equipment & Services.For Chemistry, Biotechnology, Water & Food Sciences

EQ – BOKU – an independent subsidiary of the University of Natural Resources and Life Sciences, Vienna

Web: http://eq-vibt.boku.ac.at/

EQ – BOKU We rent out state-of-the-art scientific equipment on a pay-by-use basis to research organizations, companies and universities. Additionally, we offer a full-service solution for the operation of all our scientific equipment, which is well-suited for projects in the areas of

• Chemistry• Biochemistry• Genetics• Cell biology• Molecular biology• Biotechnology• Nanobiotechnology• Bioinformatics• Food sciences• Enzyme technology• Water and sewage

Our direct support of internationally renowned researchers and scientists requires the highest standards of maintenance and operation in our equipment and services.

Standards that we can guarantee our customers.

About Us

Our collaboration partners at the University of Natural Resources and Life Sciences, Vienna, rely on numerous devices supplied by EQ:

VIBT-Extremophile Center Here molecular and cellular mechanisms of stress resistance in microorganisms are investigated with the aim of utilizing them for biotechnology.

Cellular AnalysisStudies focus on metabolomics and fluxomics relying on our latest high-end mass spectrometers.

Biomolecular AnalysisStudies on biochemical and biophysical properties of proteins and on kinetics and thermodynamics of interactions between proteins with ligands, proteins, lipids and carbohydrates.

Responsible River Modelling Center The center contributes to the synergy between a sustainable use of rivers and their protection.

Center for Preservation and Aseptic Processing The center provides advanced technologies for preservation and aseptic processing, it investigates and develops microbial inactivation mechanisms and decontamination concepts for food and biotechnology, and it offers numerous services for hygiene monitoring, process validation, microbial and quality analysis.

And our collaboration partner The University of Applied Sciences, FH Campus Wien, benefits from our experience in the area of:

BioengineeringDevelopment of recombinant production processes with various cell factories and the economic modeling of those processes.

EQ‘s Collaboration Partners

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LC-IMS-QTOF MS Achieve three dimensions of separation in a single measurement.

Current EquipmentCurrent Equipment

Advanced Mass Spectrometers

Nano-LC-MS/MS Sensitive quantification for small samples. Quantitatively analyze low femtomolar amounts of molecules even from minute sample volumes.

LC-MS/MS Sensitive, fast and robust quantification. Quantify compounds using multiple reaction monitoring concentrations over six orders of magnitude.

LC-TOF MS Perform first-step non-targeted analysis with this robust accurate mass system.

GC-MS/MS Offers the highest performance with best-in-class sensitivity.

GC-QTOF MSEquipped with high resolution and a wide mass range.

High-speed Cell Sorter

Equipped with five lasers. The instrument can analyze up to 13 size and fluorescence parameters.

Current Equipment

Choose from an extensive range of defined environmental factors including temperature, humidity, pressure, light, atmosphere (CO2, O2, N2, ozone) and salt.

Thermogravimetric Analysis / Differential Scanning Calorimetry

Measure changes in mass in a system as a function of temperature in a controlled atmosphere. Determine the organic content of a small amount of material.

Current Equipment

Benchtop Sequencer

Combine semiconductor sequencing technology with biochemistry. No need for optics and complex sequencing chemistries.

Bioreactor I + II + III

Grow up to four parallel precisely controlled cultivations of bacterial, yeast or fungal strains in small working volumes of between 0.3 and 1 liter.

Environment Emulation System

Current EquipmentCurrent Equipment

Acoustic Side Looking Doppler System

Measure flow velocities and levels in naturally turbid flows where light-based techniques reach their limitations.

Optical Turbidity Measurement System

Use the infrared duo scattered light method of Solitax sc for color independent measurements of solids and reliable sludge analysis.

Hydraulic Sediment Flume

Control the sediment transport within your experiments using the closed hydraulic circuit.

Particle Image Velocimetry Systems Use lower repetitive rates and low inter-frame times to resolve high flow velocities.

Acquire time-resolved velocity fields in the 2D and 3D space. Use high-speed applications that begin at 1000 Hz data and may reach tens of kHz.

Laser Doppler Anemometry

Non-intrusively take 1D, 2D or 3D point measurements of flow velocity, turbulence and other flow characteristics based on the Doppler effect.

Current Services Current Services

Metabolic, Environmental and Food Analysis

• Highly accurate, targeted quantification of metabolites in biotechnological and biological samples based on stable isotope internal standardization

• Non-targeted (differential) metabolomics workflows

• Determination of isotopologue and tandem mass isotopomer fractions for metabolic flux analysis

• Environmental analysis (e.g. non-target pollutant screening and tracing, exposomics)

• Method development expertise for mass spectrometry-based analysis in metabolomics and environmental analysis

• Scientific support for all stages of analytical study design, method planning and execution

Cell Sorting and Cell Analysis

• Separation based on metabolic state or other cellular properties

• Multiparameter analysis

Genomics, Transcriptomics and Proteomics

• Sequencing of genomes and transcriptomes • Diagnostics for pathogenic fungi • Characterization of pathogenic organisms for

medical data• Bioinformation analysis (metagenomes,

transcriptomes, genomes)• Proteomics and quantitative proteomics

(TMT labeling)• Glycoprotein analytics• Protein measurements & characterization• Middle-down MS of antibodies

Current Services Current Services

Micro and Nano Material Analysis

• Particle size, morphology and distribution studies

• Particle/colloid size and charge determination

• Nanoscale and microscale imaging • Biointerface and affinity measurements • Optical spectroscopy and quantitative

absorbance and fluorimetry measurements

• Cell and bacteria sample preparation for microscopy

• Contact angle and pendant drop (surface energy/tension) measurements

Biomolecular Analysis

• Determination of conformational and thermal stability of proteins and other biomolecules

• Determination of biophysical properties of proteins • Evaluation of kinetics, thermodynamics and

stoichiometry of protein-ligand, protein-protein, protein-DNA and protein-lipid interactions

• Determination of binding modes between biomolecules

• Enzyme kinetic measurements based on calorimetry• High-throughput flow-cytometry for analysis of

the kinetics and thermodynamics of interaction of proteins with biomolecules on the surface of cells, beads, nanoparticles etc.

Fermentation

• Analysis of environmental impact (stress factors) on bacteria, yeasts and filamentous fungi

• Batch cultivations• Fed-batch cultivations• Continuous cultivations

Recombinant Protein Production with Mammalian Cells (Non-GMP)

• Cultivation of mammalian cells • Batch and fed-batch cultivations• Submerse cultivation 0.6L – 10L• Packed bed reactor 1.0L • Protein purification by liquid

chromatography• Analytics based on spectroscopy

– ELISA

Current Services Current Services

Preservation and Aseptic Processing

• Conventional and advanced thermal preservation• Development of gentle, non-thermal preservation concepts

for liquid and semi-solid matrices • Advanced surface decontamination for contact materials

and solid matrices • Physical, chemical and sensory product quality analysis • Evaluation of storage stability and product shelf life testing• Monitoring of inactivation mechanisms for vegetative cells

and bacterial endospores• Study of viable but non-culturable cells and physiological

characterization of sub-lethally injured sub-populations• Validation of preservation processes and establishment of

validation concepts• Cross contamination studies• Hygiene monitoring, hygienic design, development of

cleaning, disinfection and sterilization protocols, study of biofilms on various contact surfaces, HACCP verification

• Analysis of food chain related bacteria – identity, characteristics and safety

• Tailoring of cultural and molecular methods • Case-related analytics of specific bacteria • Antibiotic resistance testing• In-vitro screening of novel antimicrobial substances• Development and safety evaluation of starter cultures and

probiotics

Thermogravimetry Analysis and Differential Scanning Calorimetry

• Organic content analysis, thermal decomposition studies of organic and hybrid materials

Water Analysis• Laser-optical measurements of flow,

sediment, bubbles, concentration, temperature etc. and flow visualization

• Flow velocities and discharge measurements and calibrations

• Turbidity and suspended solids measurements

• Velocity measurements • Displacement and tension measurements

of objects and surfaces • Development and manufacturing of

hydrodynamic models• Process and signal diagnostics, electro

technical inspections and repairs• Hydropower diagnostics • Static pressure and water level

measurements• Temporal and long-term data acquisition

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EQ - BOKU - VIBT (Equipment - BOKU Vienna Institute of Bio Technology) - GmbH

Muthgasse 181190 Vienna, Austria

Ingrid Döller-Diem, ManagerTel: +43 / (0)1 / 47654 – 99304E-mail: ingrid.doeller-diem@boku.ac.at

Dr. Hannes Diem, General ManagerTel: +43 / (0)699 / 19 37 5086E-mail: hannes.diem@boku.ac.at

Company registration number: FN 347650aVAT number: ATU66085611

Web: http://eq-vibt.boku.ac.at

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