EPPL 2001 report - wyomingextension.org · Laboratory (EPPL).....4 Table 1. EPPL total number of samples submitted from 1991-2001.....5 Table 2. EPPL diagnostic samples for 2001.....14

University of Wyoming Cooperative Extension Service Plant Pathology Laboratory Report

Plant Sample Submissions and Diagnostics for 2001

Prepared by

Stephan C. Briere, M. Sc., Associate Research Scientist Gary D. Franc, Ph. D., Extension Plant Pathology Specialist

MP 110-02

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Table of Contents Preface: How to use this report ....................................................................................... 3 Diagnostics report for the University of Wyoming Extension Plant Pathology Laboratory (EPPL)........................................................................................................... 4 Table 1. EPPL total number of samples submitted from 1991-2001................................ 5 Table 2. EPPL diagnostic samples for 2001.................................................................. 14 Table 3. EPPL field crop samples 2001......................................................................... 34 Table 4. EPPL tree and shrub samples 2001 ................................................................ 48 Table 5. EPPL home and garden samples 2001 ........................................................... 54 Table 6. EPPL turf samples 2001 .................................................................................. 56 Table 7. EPPL commercial horticulture samples 2001 .................................................. 57 Table 8. EPPL non-plant and plant identification samples 2001.................................... 58 Table 9. EPPL high plains regional Cercospora survey 2001........................................ 59 Table 10. EPPL potato disease testing for the Potato Certification Association of NE 2001 ................................................................................ 63 EPPL 1991-2000 report survey questionnaire............................................................... 69 EPPL sample form......................................................................................................... 70

2

Preface: How to use this report This University of Wyoming Cooperative Extension Service Plant Pathology Report was produced with two objectives in mind.

• To provide a detailed report on the numbers and types of samples that were received and processed through the Extension Plant Pathology Lab (EPPL) since its inception in 1991 (Table1) and expanded information on samples received during 2001 (Tables 2-10).

• To serve as a reference for people who need to know what diseases have been

identified and reported in their particular Wyoming county. There are 10 tables in this document which are generally sorted by type of host plant and then grouped by State or Wyoming county. This will facilitate selection of the data set that best suits individual needs.

3

University of Wyoming Cooperative Extension Service Plant Pathology Laboratory Report

Plant Sample Submissions and Diagnostics for 2001

The University of Wyoming Cooperative Extension Service Plant Pathology Laboratory (EPPL) extends from the university campus to the field and represents UW’s Extension Plant Pathology Program. Although considerable educational effort is focused on field research, demonstration and disease recognition, and/or training plots, a significant portion of the EPPL programming effort is directed towards a fee-based diagnostic service for plant samples submitted to the EPPL. Samples for Wyoming clients are submitted by Cooperative Extension Service educators and their clients, Master Gardeners, growers, landscape professionals, crop advisors, and organizations such as the Wyoming Seed Certification Service and the Potato Certification Association of Nebraska. Once diagnosis of the sample is completed, results and/or educational information is returned to the client. This information includes practical, sample- specific, disease management recommendations. Plant samples also are submitted from surrounding states by university employees, producers, and crop advisors for diagnosis and/or assistance with recommendations for disease control. In addition, the EPPL recently started receiving “virtual samples” submitted as digital images (indicated by a “D” following the sample number). Digital images are useful tools for keying in on certain plant problems. Use of digital images promotes regionalization of diagnostic efforts among neighboring states. The number of digital images submitted has increased each year since this service became available. We processed a total of 25 digital samples in 2001. Samples are also submitted in support of various pest surveys as part of the extension programming effort. For example, a survey is being conducted in Wyoming and neighboring states to determine the effectiveness of various fungicides for Cercospora beticola, the causal agent of Cercospora leaf spot of sugar beets. The survey includes infected sugar beets recovered from growers’ fields in Colorado, Montana, Nebraska, and Wyoming. Diagnostic results are being used to limit the use of ineffective products. Survey samples submitted during 2001 are listed as numbers 20001.319 to 2001.402. Diagnostic results are also being used in support of seed certification efforts (see 2001.150 to 2001.314 and several additional EPPL samples). This report summarizes statistics of the 410 plant samples and related diagnostic results for 2001. Various statistics for prior years (1991 through 2000) are summarized in Table 1 and are available at the University of Wyoming Cooperative Extension Web site or the UW bulletin room (see publication #B-1111). For additional information on the Cooperative Extension Service plant pathology program, contact Gary Franc at (307) 766-2397 ([email protected]) or the Department of Plant Sciences office at (307) 766-3103.

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Table 1. Extension Plant Pathology Lab Total number of samples submitted from 1991-2001. The total number of samples recorded over the period of 1991 to 2001 was 2,830. A total of 410 samples were submitted in 2001. (Note: Sample types are denoted by letters where: F = field crops; S = trees and shrubs; G = home garden and house plants; T = turf; H = commercial horticulture; O = non-plant samples and plant identification; R = research samples; P = pest survey samples)

TYPE OF SAMPLE

LOCATION

COUNTY/STATE

YEAR

NO. OF

SAMPLES

F

S

G

T

H

O

R

P

SAMPLE TOTALS

1991-2001

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

533315249

53102215193205236319410

2,830

58104162

1021197736222329

561

340129

562551265082637191

984

965018

619121018101615

270

2816

265297

111110

107

0 0 0 0 0 4 4

12 3 5 8

36

11

8 7 1 0 0 4 0 3 9 7

50

0245612

1112

70

50

06000

1513739

112177250772

ALBANY COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

693016

414

722402210

8242

230000010006

5417

9294

162616

84

165

97504357402

46

41020001002

10

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 2 0 2

02201015200

13

000000000000

5

TYPE OF SAMPLE

LOCATION

COUNTY/STATE

YEAR

NO. OF

SAMPLES

F

S

G

T

H

O

R

P

BIG HORN COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

1012

213

183

10922

72

020003002209

65201001301

19

310110010018

1 20010002006

0 0 0 0 0 3 3 7 2 0 0

15

0 2 0 0 0 0 0 0 0 0 0 2

000000000000

00000

1201000

13

CAMPBELL COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

2613

012

311213152332

168

000010000001

18901106

1013162397

63000011043

18

21000010233

12

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 1 0 0 0 3 4

000000000000

00000

3132000

36

CARBON COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

19343

11232320

52

100050000006

152435121320

38

310010000005

000000000000

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

000000000000

000001110003

CONVERSE COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

34000411101

15

000000000000

320000000016

010000000001

010000001002

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

000000000000

000004110006

6

TYPE OF SAMPLE

LOCATION

COUNTY/STATE

YEAR

NO. OF

SAMPLES

F

S

G

T

H

O

R

P

CROOK COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

52210603001

20

211000010005

110000000013

101100000003

100000000001

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

000000000000

000006020008

FREMONT COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

8029

911

1025424

147

410101000108

5516

501214102

87

1410

300001210

31

411001100008

0 0 0 0 0 0 0 0 0 0 2 2

3 1 0 0 0 0 0 0 1 0 0 5

000000000000

000006000006

GOSHEN COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

3222

617

151312

512

6131

104201221222

28

138404000003

32

62011000111

13

38001010000

13

0 0 0 0 0 0 0 0 1 3 0

4

0 0 0 0 0 0 0 0 0 1 0 1

000001021307

00000

1210

9020

33

HOT SPRINGS COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

218311329489

69

201000010105

107111226477

48

411000010007

200000010003

0 0 0 0 0 0 0 0 0 0 2

2

3 0 0 0 0 0 0 0 0 0 0 3

000000000000

000001000001

7

TYPE OF SAMPLE

LOCATION

COUNTY/STATE

YEAR

NO. OF

SAMPLES

F

S

G

T

H

O

R

P

JOHNSON COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

82100736143

35

101002000004

72000113112

18

000000120003

000000100113

0 0 0 0 0 0 0 0 0 1 0

1

0 0 0 0 0 0 0 0 0 0 0 0

000000000101

000004010005

LARAMIE COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

2721

236

201319

319

7140

73015010054

26

167000105383

43

35201101010

14

100101010307

0 0 0 0 0 0 0 0 0 0 0

0

0 0 0 0 0 0 0 0 0 1 0 1

000100000102

06000

171212

000

47

LINCOLN COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

1911

212013062

47

100000100103

103001002022

20

66000001000

13

200110000004

0 0 0 0 0 0 0 0 0 0 0

0

0 2 2 0 0 0 0 0 0 3 0 7

000000000000

000000000000

NATRONA COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

483016

416

27

10446

147

100000000001

332112

311

157224

101

97414001102

29

410010101109

0 0 0 0 0 0 0 1 0 1 0

2

1 1 0 0 0 0 0 0 0 0 0 2

000000000000

000001110003

8

TYPE OF SAMPLE

LOCATION

COUNTY/STATE

YEAR

NO. OF

SAMPLES

F

S

G

T

H

O

R

P

NIOBRARA COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

51241

1223036

39

400200010007

00220100025

12

100011000014

000000000000

0 0 0 0 0 0 0 0 0 0 0

0

0 1 0 0 0 0 0 0 0 0 0 1

000000000101

00000

1022000

14

PARK COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

71105164

1314267737241818

567

1987

154486

7127

973

395

3815

54505287

11100

121220201122

25

11121013411

16

0 0 0 0 0 1 0 2 0 0 0

3

1 1 2 1 0 0 0 0 2 1 1 9

000000010001

00000

1701000

18

PLATTE COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

2350095605

1045

021002010309

21200000007

12

000000000202

000000000011

0 0 0 0 0 0 0 0 0 0 1 1

0 0 2 0 0 0 0 0 0 0 1 3

000000000000

00000755000

17

SHERIDAN COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

24242

122213

5042

90

311110000108

150216441021

36

41005121010

15

100000200003

0 0 0 0 0 0 1 2 0 0 1 4

1 0 0 0 0 0 3 0 0 0 0 4

001000000001

00000

1711000

19

9

TYPE OF SAMPLE

LOCATION

COUNTY/STATE

YEAR

NO. OF

SAMPLES

F

S

G

T

H

O

R

P

SUBLETTE COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

146100130210

28

100000000001

125100000200

20

110001100105

000000100001

0 0 0 0 0 0 0 0 0 0 0

0

0 0 0 0 0 0 0 0 0 0 0 0

000000100001

000000000000

SWEETWATER

COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

21312021216

21

000000102003

21310011003

12

000000000011

000000000112

0 0 0 0 0 0 0 0 0 0 0

0

0 0 0 0 0 0 0 0 0 0 1 1

000020000002

000000000000

TETON

COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

163243206003

39

000000000000

132243206003

35

210000000003

100000000001

0 0 0 0 0 0 0 0 0 0 0

0

0 0 0 0 0 0 0 0 0 0 0 0

000000000000

000000000000

UINTA COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

102110410099

37

001000000001

51010410076

25

410000000106

000000000101

0 0 0 0 0 0 0 0 0 0 0

0

1 0 0 0 0 0 0 0 0 0 1 2

000000000011

000000000011

10

TYPE OF SAMPLE

LOCATION

COUNTY/STATE

YEAR

NO. OF

SAMPLES

F

S

G

T

H

O

R

P

WASHAKIE COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

123131304423

36

000100001002

73121203321

25

500001000028

000000010001

0 0 0 0 0 0 0 0 0 0 0

0

0 0 0 0 0 0 0 0 0 0 0 0

000000000000

000000000000

WESTON COUNTY

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

82203866673

51

000000020002

51102164451

30

31001200121

11

000000001012

0 0 0 0 0 0 0 0 0 0 0

0

0 0 1 0 0 0 0 0 0 0 0 1

000000000000

000005000005

ALASKA

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

000010032006

000000000000

000000000000

000000000000

000000000000

0 0 0 0 0 0 0 0 0 0 0

0

0 0 0 0 0 0 0 0 0 0 0 0

000010032006

000000000000

CALIFORNIA

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

000000100001

000000100001

000000000000

000000000000

000000000000

0 0 0 0 0 0 0 0 0 0 0

0

0 0 0 0 0 0 0 0 0 0 0 0

000000000000

000000000000

11

TYPE OF SAMPLE

LOCATION

COUNTY/STATE

YEAR

NO. OF

SAMPLES

F

S

G

T

H

O

R

P

COLORADO

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

00000201

509138

182

000002011004

000000000000

000000000000

000000000000

0 0 0 0 0 0 0 0 0 0 0

0

0 0 0 0 0 0 0 0 0 0 0 0

000000001001

00000000

489138

177

MICHIGAN

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

000100000102

000000000000

000000000000

000000000000

000000000000

0 0 0 0 0 0 0 0 0 0 0

0

0 0 0 0 0 0 0 0 0 1 0 1

000100000001

000000000000

MONTANA

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

00000000

24121147

000000000000

000000000000

000000000000

000000000000

0 0 0 0 0 0 0 0 0 0 0

0

0 0 0 0 0 0 0 0 0 0 0 0

000000000000

00000000

24121147

NEBRASKA

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

00132010

5072

221350

0000000050

2025

000000000000

000000000000

000000000000

0 0 0 0 0 0 0 0 0 0 0

0

0 0 0 0 0 0 0 0 0 0 0 0

00132000500

11

00000010

4072

201314

12

TYPE OF SAMPLE

LOCATION

COUNTY/STATE

YEAR

NO. OF

SAMPLES

F

S

G

T

H

O

R

P

WASHINGTON

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

000000001102

000000000000

000000000000

000000000000

000000000000

0 0 0 0 0 0 0 0 0 0 0

0

0 0 0 0 0 0 0 0 0 0 0 0

000000001102

000000000000

UNKNOWN ORIGIN

1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001

TOTAL

200001000003

000001000001

000000000000

000000000000

100000000001

0 0 0 0 0 0 0 0 0 0 0

0

1 0 0 0 0 0 0 0 0 0 0 1

000000000000

000000000000

GRAND TOTAL

2,830 561 984 272 107 34 50 50 772

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Table 2. EPPL diagnostic samples for 2001 County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen Alfalfa Park, WY 2001.111D Moore, Mike 7/25/2001 Vestigial Flower and Branched Raceme (genetic abnormality)

Apple Campbell, WY 2001.072D Thompson, Janet 6/7/2001 Poor Establishment (presumptive) Campbell, WY 2001.078 Thompson, Janet 6/18/2001 Cytospora Canker Cytospora sp. Campbell, WY 2001.107 Thompson, Janet 7/19/2001 Fire Blight Erwinia amylovora Goshen, WY 2001.126 Tatman, Wayne 8/23/2001 Fire Blight suspected But Not Detected, Fruit had Insect Damage

Ash Park, WY 2001.122 Holder, Bobbie 8/10/2001 Ash Leafspot Phyllosticta fraxinicola

Aspen Hot Springs, 2001.099D Langbehn, Jerry 7/3/2001 Probable Ink Spot Damage, difficult to determine from images. Hot Springs, 2001.119 Langbehn, Jerry 8/6/2001 Drought Injury Uinta, WY 2001.144 Uinta CES 9/18/2001 Shoot Blight and Cytospora Canker (canker presumptive based on Venturia tremulae

Aspen, Apple and Maple Uinta, WY 2001.106 Uinta CES 7/17/2001 Leaf Scorch and Drought Injury

Aster Natrona, WY 2001.115 Logue, Judy 7/30/2001 Fusarium Wilt and Yellows Fusarium sp.

Basil Sheridan, WY 2001.102 7/11/2001 Severe Thrips Infestation

Bean, Dry Goshen, WY 2001.117 Cecil, Jack 8/1/2001 Research sample: Culture and Identify Bacterial Isolates Probable

14

County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen

Bean, Pinto Park, WY 2001.137 Wyoming Seed 8/9/2001 Bacterial Bean Blight Not Detected, No Fusarium Detected

Birch Campbell, WY 2001.038 Thompson, Janet 4/27/2001 Suspect Scale insects not found, some type of residue.

Caragana Weston, WY 2001.086 Taylor, Bill 6/22/2001 Poor Establishment and Vigor

Chokecherry Platte, WY 2001.128D Mount, Dallas 8/30/2001 Peach Tree Borer and Crown Rot, presumptive based on images

Clover, Red Park, WY 2001.085 Allied Seed LLC 6/20/2001 Fusarium Root & Crown Rot Fusarium sp.

Corn Goshen, WY 2001.130 Tatman, Wayne 8/29/2001 Purple Leaf Sheath (presumptive) Various Saprophytic Fungi

Cottonwood and Aspen Campbell, WY 2001.055 5/18/2001 Cytospora Canker and Hail Damage Cytospora sp.

Elm, Chinese Niobrara, WY 2001.121 Jensen, Tammie 8/13/2001 Insect Damage suspected, Forwarded to Mike Brewer (Ext. Ento.)

False Astilbe Park, WY 2001.103 7/18/2001 Leaf Scorch (due to dry hot conditions) and Poor Establishment

Ficus Niobrara, WY 2001.032 Jensen, Tamra 4/10/2001 Leaf Drop Due to Management Problems

Fir Lincoln, WY 2001.041 Brown, Gurn 4/27/2001 Lichens Growing on Branch

15


Fungal Growth Platte, WY 2001.097D Mount, Dallas 7/6/2001 Fungal Growth In Wet Arena Soil/Manure on Floor , Saprophytic

Geranium Hot Springs, 2001.082 Langbehn, Jerry 6/19/2001 Environmental Injury suspected, Herbicide Injury Not Suspected

Hedge Campbell, WY 2001.316D Thompson, Janet 10/11/2001 Leaf Scorch Associated with Heat Stress

Hollyhock Albany, WY 2001.104 7/19/2001 Severe Mite Infestation Albany, WY 2001.108 Moline, Brett 7/24/2001 Endophyllum Rust and Mite Infestation Endophyllum tuberculatum Washakie, WY 2001.067 Gill, Jim 6/6/2001 Probable Iron Chlorosis and Insect Problem

Juniper Park, WY 2001.056 Holder, Bobbie 5/18/2001 Spruce Spider Mite Infestation Uinta, WY 2001.132 Uinta Weed & 8/31/2001 Cedar Apple Rust Galls Gymnosporangium sp.

Lilac Albany, WY 2001.149 10/2/2001 Powdery Mildew Microsphaera penicillata Park, WY 2001.062 Holder, Bobbie 5/30/2001 Frost Damage Park, WY 2001.090 Holder, Bobbie 6/29/2001 Probable Frost Injury Platte, WY 2001.066D Mount, Dallas 6/6/2001 Frost Damage

Lilac, Spruce and Elm Albany, WY 2001.093 Moline, Brett 7/3/2001 Herbicide Injury

Linden, Spotted Platte, WY 2001.060D Mount, Dallas 5/29/2001 Cold Temperature or Herbicide Injury (presumptive)

Mold Identification Campbell, WY 2001.142 Daniels, Tanya 9/14/2001 Penicillium sp. Penicillium sp.

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Moldy Dry Wall Sample Campbell, WY 2001.039 Daniels, Tanya 4/27/2001 Alternaria and Ulocladium spp. Fungi Recovered Alternaria sp.

Mountain Ash Campbell, WY 2001.114 Thompson, Janet 7/27/2001 Drought Injury and Mite Infestation Fremont, WY 2001.116 Cunningham, Ron 7/30/2001 Fire Blight Suspected but Not Detected, Mite Infestation Found

Mushroom ID Campbell, WY 2001.074D Thompson, Janet 6/13/2001 Type of Russula Mushroom (presumptive ID from photograph)

Oak Campbell, WY 2001.110D Thompson, Janet 7/24/2001 Leaf Scorch / Drought Stress

Oat Laramie, WY 2001.113 Rosenlund, Phil 7/31/2001 Ascochyta Leaf Blight Ascochyta sp.

Organic Debris Natrona, WY 2001.134 9/4/2001 Sample Contained Decayed/Composted Plant Material

Pepper, Sweet Bell Fremont, WY 2001.046 Cunningham, Ron 5/1/2001 No Disease Detected; Probable Sulfur Dioxide/Ethylene Damage

Petunia Goshen, WY 2001.028 Kaufman, Ron 4/6/2001 No Pathogens Detected - Probable Abiotic Problem

Pine Campbell, WY 2001.042 Thompson, Janet 4/30/2001 Winter Injury Campbell, WY 2001.088D Thompson, Janet 6/27/2001 Drought Injury Campbell, WY 2001.318 Janet, Thompson 10/26/2001 None - Unable to determine cause of problem Hot Springs, 2001.148D Langbehn, Jerry 10/2/2001 Chronic Stress Possibly Attributed to Overwatering and Lack of Laramie, WY 2001.035 Koch, Les 4/20/2001 Dothistroma pini Not Detected Natrona, WY 2001.051 Alpine 5/11/2001 Winter Injury/Dessication

17

County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen Natrona. WY 2001.076 Heald, Tom 6/15/2001 Brown Felt Blight and Slime Mold Fruiting Structures Neopeckia coulteri Niobrara, WY 2001.409 Jensen, Tammie 12/6/2001 Twig Beetles Found (they are still active) Park, WY 2001.044 Holder, Bobbie 5/1/2001 Winter Injury/Dessication Platte, WY 2001.089D Mount, Dallas 6/27/2001 Propable White Pine Weevil Damage (based on image and Uinta, WY 2001.045 Uinta CES 5/1/2001 White Pine Blister Rust Cronartium ribicola Washikie, WY 2001.001 Gill, Jim 1/8/2001 Dessication/Moisture Stress n/a

Pine and Aspen Teton, WY 2001.109 Martin, Mary 7/23/2001 Pine Needle Scale (Pine) and Earwigs Associated with Aspen Leaves

Plant ID Park, WY 2001.133 Holder, Bobbie 8/20/2001 Narrowleaf Cottonwood, Populus angustifolia Sweetwater, 2001.091 Sweetwater CO. 7/2/2001 Western Peppergrass, Lepidium montana

Plum Niobrara, WY 2001.069 Jensen, Tammie 6/11/2001 Fingergall Mites

Poplar Crook, WY 2001.139 9/11/2001 Iron Chlorosis, Herbicide Damage Not Likely Fremont, WY 2001.057D Malcom, 5/23/2001 Frost Injury

Potato Campbell, WY 2001.124D Thompson, Janet 8/14/2001 Potato Leaf Roll Virus, presumptive from image PLRV Lincoln, WY 2001.317 Brown, Gurn 10/26/2001 Common Scab Streptomyces scabies Natrona, WY 2001.129 Logue, Judy 8/30/2001 Soft Rot with Possible association with Western Leak Erwinia cartovora NEBRASKA 2001.003 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.004 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.005 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.006 PCAN 1/25/2001 Late Blight NOT Detected

18

County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen NEBRASKA 2001.007 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.008 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.009 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.010 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.011 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.012 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.013 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.014 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.015 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.016 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.017 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.018 PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.019A PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.019B PCAN 1/25/2001 Late Blight NOT Detected NEBRASKA 2001.022 PCAN 2/15/2001 Late Blight Not Detected NEBRASKA 2001.023 PCAN 2/27/2001 Late Blight Not Detected NEBRASKA 2001.150 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.151 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.152 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.153 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.154 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.155 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.156 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.157 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.158 PCAN 7/30/2001 ELISA Virus/Bacteria Screening

19

County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen NEBRASKA 2001.159 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.160 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.161 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.162 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.163 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.164 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.165 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.166 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.167 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.168 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.169 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.170 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.171 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.172 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.173 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.174 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.175 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.176 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.177 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.178 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.179 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.180 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.181 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.182 PCAN 7/30/2001 ELISA Virus/Bacteria Screening

20

NEBRASKA 2001.183 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.184 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.185 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.186 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.187 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.188 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.189 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.190 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.191 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.192 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.193 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.194 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.195 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.196 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.197 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.198 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.199 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.200 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.201 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.202 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.203 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.204 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.205 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.206 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.207 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.208 PCAN 7/30/2001 ELISA Virus/Bacteria Screening

21

County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen NEBRASKA 2001.209 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.210 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.211 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.212 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.213 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.214 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.215 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.216 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.217 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.218 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.219 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.220 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.221 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.222 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.223 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.224 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.225 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.226 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.227 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.228 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.229 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.230 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.231 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.232 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.233 PCAN 7/30/2001 ELISA Virus/Bacteria Screening

22


23


24

County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen NEBRASKA 2001.284 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.285 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.286 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.287 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.288 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.289 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.290 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.291 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.292 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.293 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.294 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.295 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.296 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.297 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.298 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.299 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.300 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.301 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.302 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.303 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.304 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.306 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.307 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.308 PCAN 7/30/2001 ELISA Virus/Bacteria Screening

25

County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen NEBRASKA 2001.309 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.310 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.311 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.312 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.313 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.314 PCAN 7/30/2001 ELISA Virus/Bacteria Screening NEBRASKA 2001.404 11/6/2001 Late Blight NOT Detected, Fusarium sp. Isolated from tuber Fusarium sp. NEBRASKA 2001.405 11/30/2001 Late Blight NOT Detected, water mold isolated but not pathogenic Weston, WY 2001.408 Taylor, Bill 12/5/2001 Lenticel Soft Rot, presumptive Erwinia carotovora

Raspberry Park, WY 2001.135 Holder, Bobbie 9/5/2001 Yellow Rust (presuptive based on symptoms and single spore found) Phragmidium rubi-idaei

Red Cedar Hot Springs, 2001.120 Langbehn, Jerry 8/6/2001 Juniper Borer Damage

Rhubarb Sweetwater, 2001.083D Harrington, 6/21/2001 Root and Crown Rot, presumptive based on symptoms

Spirea Johnson, WY 2001.087 Horn, Blaine 6/22/2001 Probable Frost/Cold Injury

Spruce Albany, WY 2001.315 Moline, Brett 10/4/2001 Probable Moisture Stress and Fertility Problems Big Horn, WY 2001.098D Cooper, Troy 7/6/2001 Diagnosis Not Conclusive due to Poor Image, possible Campbell, WY 2001.025 Thompson, Janet 3/14/2001 Insect Damage - White Pine Weevil Campbell, WY 2001.026D Thompson, Janet 3/21/2001 Winter Injury Campbell, WY 2001.036 Thompson, Janet 4/24/2001 Winter Injury and Possible Salts Injury Campbell, WY 2001.040 Thompson, Janet 4/27/2001 Probable Loss of Vigor and Salts Problem

26

County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen Campbell, WY 2001.058 Thompson, Janet 5/23/2001 Winter Injury Campbell, WY 2001.073D Thompson, Janet 6/8/2001 Planted Too Deeply and possible Physical Damage to Growing Campbell, WY 2001.095 Thompson, Janet 7/5/2001 Unable to Determine Cause of Problem - asked for phisical sample, Campbell, WY 2001.100 Thompson, Janet 7/9/2001 Overwatering and Oedema Symptoms Campbell, WY 2001.101 Thompson, Janet 7/9/2001 Drought / Heat Stress, Possible Frost Injury this Spring Campbell, WY 2001.131 Thompson, Janet 8/30/2001 Poor Vigor and Possible Salts Problem Campbell, WY 2001.146 Thompson, Janet 9/26/2001 Poor Establishment and Loss of Vigor (new planting) Converse, WY 2001.068 6/6/2001 Probable Drought Injury / Winter Injury Goshen, WY 2001.020 Kaufman, Ron 2/15/2001 Winter Injury n/a Hot Springs, 2001.140D Langbehn, Jerry 9/11/2001 Problem Undetermined, Possible Related to Canker or Root Inury Johnson, WY 2001.034 Horn, Blaine 4/17/2001 Winter Injury and Possible Salts Injury Lincoln, WY 2001.143 Brown, Gurn 9/14/2001 Injury to Growing Point and Drought Stress Niobrara, WY 2001.053 Jensen, Tamra 5/15/2001 Dessication/Drought Injury Niobrara, WY 2001.147 Jensen, Tammie 9/27/2001 Overwatering Injury and Pine Scale Park, WY 2001.024 Holder, Bobbie 3/9/2001 Probable Management Problems Park, WY 2001.052 5/11/2001 Fertility Problem and Possible Lack of Water Park, WY 2001.065 Holder, Bobbie 6/1/2001 Cytospora Canker Cytospora kunzei Park, WY 2001.077 Cody Tree 6/18/2001 Probable Salts Injury and Poor Vigor Park, WY 2001.092 Holder, Bobbie 7/2/2001 No Disease Detected - Possible Chemical or Environmental Injury Platte, WY 2001.029 Mount, Dallas 4/6/2001 Winter Injury Platte, WY 2001.030 Mount, Dallas 4/6/2001 Winter Injury Platte, WY 2001.094 Mount, Dallas 7/3/2001 Cytospora Canker of Spruce (presumptive) cankers present but spores Cytospora kunzei Sheridan, WY 2001.002 1/16/2001 Salts (Soil/Water) Problem; presumtive n/a Sweetwater, 2001.063 Harrington, 5/31/2001 Presumtive Salts Injury Sweetwater, 2001.064 Harrington, 5/31/2001 Drought Injury/Winter Injury and Pine Needle Scale Infestation

27

County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen Teton, WY 2001.406 12/3/2001 Poor Vigor and Establishment Problems Teton, WY 2001.407 12/3/2001 Transplant Shock and Poor Establishment Uinta, WY 2001.027 Uinta CES 4/3/2001 Winter Injury Uinta, WY 2001.050 Uinta CES 5/10/2001 Winter Injury/Dessication Washakie, WY 2001.021 2/26/2001 Possible Canker Problem and/or Root Problems

Spruce and Pine Albany, WY 2001.080 Moline, Brett 6/19/2001 No Disease Detected, Probably Drought/Winter Injury Campbell, WY 2001.037 Thompson, Janet 4/24/2001 Winter / Dessication Injury

Sugar Beet Banner, NE 2001.340 Kerr, Eric 10/26/2001 01-22 No Isolates recovered from sample Banner, NE 2001.341 Kerr, Eric 10/26/2001 01-23 1 Isolates recovered from sample (A) Box Butte, NE 2001.329 Kerr, Eric 10/26/2001 01-11 2 Isolates recovered from sample (A-B) Box Butte, NE 2001.330 Kerr, Eric 10/26/2001 01-12 5 Isolates recovered from sample (A-E) Box Butte, NE 2001.331 Kerr, Eric 10/26/2001 01-13 1 Isolates recovered from sample (A) Cheyenne, NE 2001.401 Kerr, Eric 10/26/2001 01-83 No Isolates recovered from sample COLORADO 2001.362 Kerr, Eric 10/26/2001 01-44 4 Isolates recovered from sample (A-D) COLORADO 2001.363 Kerr, Eric 10/26/2001 01-45 2 Isolates recovered from sample (A-B) COLORADO 2001.364 Kerr, Eric 10/26/2001 01-46 6 Isolates recovered from sample (A-F) COLORADO 2001.365 Kerr, Eric 10/26/2001 01-47 6 Isolates recovered from sample (A-F) COLORADO 2001.366 Kerr, Eric 10/26/2001 01-48 2 Isolates recovered from sample (A-B) COLORADO 2001.367 Kerr, Eric 10/26/2001 01-49 1 Isolates recovered from sample (A) COLORADO 2001.368 Kerr, Eric 10/26/2001 01-50 No Isolates recovered from sample COLORADO 2001.369 Kerr, Eric 10/26/2001 01-51 No Isolates recovered from sample COLORADO 2001.370 Kerr, Eric 10/26/2001 01-52 2 Isolates recovered from sample (A-B)

28

County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen COLORADO 2001.371 Kerr, Eric 10/26/2001 01-53 6 Isolates recovered from sample (A-F) COLORADO 2001.372 Kerr, Eric 10/26/2001 01-54 4 Isolates recovered from sample (A-D) COLORADO 2001.373 Kerr, Eric 10/26/2001 01-55 2 Isolates recovered from sample (A-B) COLORADO 2001.374 Kerr, Eric 10/26/2001 01-56 No Isolates recovered from sample COLORADO 2001.375 Kerr, Eric 10/26/2001 01-57 No Isolates recovered from sample COLORADO 2001.376 Kerr, Eric 10/26/2001 01-58 2 Isolates recovered from sample (A-B) COLORADO 2001.377 Kerr, Eric 10/26/2001 01-59 5 Isolates recovered from sample (A-E) COLORADO 2001.378 Kerr, Eric 10/26/2001 01-60 7 Isolates recovered from sample (A-G) COLORADO 2001.379 Kerr, Eric 10/26/2001 01-61 3 Isolates recovered from sample (A-C) COLORADO 2001.380 Kerr, Eric 10/26/2001 01-62 2 Isolates recovered from sample (A-B) COLORADO 2001.381 Kerr, Eric 10/26/2001 01-63 No Isolates recovered from sample COLORADO 2001.382 Kerr, Eric 10/26/2001 01-64 1 Isolates recovered from sample (A) COLORADO 2001.383 Kerr, Eric 10/26/2001 01-65 No Isolates recovered from sample COLORADO 2001.384 Kerr, Eric 10/26/2001 01-66 6 Isolates recovered from sample (A-F) COLORADO 2001.385 Kerr, Eric 10/26/2001 01-67 4 Isolates recovered from sample (A-D) COLORADO 2001.386 Kerr, Eric 10/26/2001 01-68 3 Isolates recovered from sample (A-C) COLORADO 2001.387 Kerr, Eric 10/26/2001 01-69 5 Isolates recovered from sample (A-E) COLORADO 2001.391 Kerr, Eric 10/26/2001 01-73 No Isolates recovered from sample COLORADO 2001.392 Kerr, Eric 10/26/2001 01-74 3 Isolates recovered from sample (A-C) COLORADO 2001.393 Kerr, Eric 10/26/2001 01-75 4 Isolates recovered from sample (A-D) COLORADO 2001.394 Kerr, Eric 10/26/2001 01-76 No Isolates recovered from sample COLORADO 2001.395 Kerr, Eric 10/26/2001 01-77 4 Isolates recovered from sample (A-D) COLORADO 2001.396 Kerr, Eric 10/26/2001 01-78 2 Isolates recovered from sample (A-B) COLORADO 2001.397 Kerr, Eric 10/26/2001 01-79 4 Isolates recovered from sample (A-D)

29

County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen COLORADO 2001.398 Kerr, Eric 10/26/2001 01-80 1 Isolates recovered from sample (A) COLORADO 2001.399 Kerr, Eric 10/26/2001 01-81 3 Isolates recovered from sample (A-C) COLORADO 2001.400 Kerr, Eric 10/26/2001 01-82 8 Isolates recovered from sample (A-H) COLORADO 2001.402 Kerr, Eric 10/26/2001 01-84 3 Isolates recovered from sample (A-C) COLORADO 2001.403 Kerr, Eric 10/26/2001 01-85 No Isolates recovered from sample MONTANA 2001.319 Kerr, Eric 10/26/2001 01-01 7 Isolates recovered from sample (A-G) MONTANA 2001.320 Kerr, Eric 10/26/2001 01-02 6 Isolates recovered from sample (A-F) MONTANA 2001.321 Kerr, Eric 10/26/2001 01-03 6 Isolates recovered from sample (A-F) MONTANA 2001.322 Kerr, Eric 10/26/2001 01-04 5 Isolates recovered from sample (A-E) MONTANA 2001.323 Kerr, Eric 10/26/2001 01-05 7 Isolates recovered from sample (A-G) MONTANA 2001.324 Kerr, Eric 10/26/2001 01-06 3 Isolates recovered from sample (A-C) MONTANA 2001.325 Kerr, Eric 10/26/2001 01-07 6 Isolates recovered from sample (A-F) MONTANA 2001.326 Kerr, Eric 10/26/2001 01-08 2 Isolates recovered from sample (A and C) MONTANA 2001.327 Kerr, Eric 10/26/2001 01-09 7 Isolates recovered from sample (A-H) MONTANA 2001.328 Kerr, Eric 10/26/2001 01-10 8 Isolates recovered from sample (A-H) MONTANA 2001.354 Kerr, Eric 10/26/2001 01-36 5 Isolates recovered from sample (A-E) Morrill, NE 2001.334 Kerr, Eric 10/26/2001 01-16 No Isolates recovered from sample Morrill, NE 2001.335 Kerr, Eric 10/26/2001 01-17 3 Isolates recovered from sample (A-C) Morrill, NE 2001.336 Kerr, Eric 10/26/2001 01-18 2 Isolates recovered from sample (A-B) Morrill, NE 2001.337 Kerr, Eric 10/26/2001 01-19 No Isolates recovered from sample Morrill, NE 2001.338 Kerr, Eric 10/26/2001 01-20 6 Isolates recovered from sample (A-F) Morrill, NE 2001.339 Kerr, Eric 10/26/2001 01-21 10 Isolates recovered from sample (A-K) NEBRASKA 2001.355 Kerr, Eric 10/26/2001 01-37 3 Isolates recovered from sample (A-C) NEBRASKA 2001.356 Kerr, Eric 10/26/2001 01-38 7 Isolates recovered from sample (A-G) NEBRASKA 2001.357 Kerr, Eric 10/26/2001 01-39 2 Isolates recovered from sample (A-B)

30

County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen NEBRASKA 2001.358 Kerr, Eric 10/26/2001 01-40 6 Isolates recovered from sample (A-F) NEBRASKA 2001.359 Kerr, Eric 10/26/2001 01-41 3 Isolates recovered from sample (A-C) NEBRASKA 2001.360 Kerr, Eric 10/26/2001 01-42 3 Isolates recovered from sample (A-C) NEBRASKA 2001.361 Kerr, Eric 10/26/2001 01-43 2 Isolates recovered from sample (A-B) NEBRASKA 2001.388 Kerr, Eric 10/26/2001 01-70 2 Isolates recovered from sample (A-B) NEBRASKA 2001.389 Kerr, Eric 10/26/2001 01-71 6 Isolates recovered from sample (A-F) NEBRASKA 2001.390 Kerr, Eric 10/26/2001 01-72 4 Isolates recovered from sample (A-D) Scotts Bluff, 2001.342 Kerr, Eric 10/26/2001 01-24 5 Isolates recovered from sample (A-E) Scotts Bluff, 2001.343 Kerr, Eric 10/26/2001 01-25 1 Isolates recovered from sample (A) Scotts Bluff, 2001.344 Kerr, Eric 10/26/2001 01-26 2 Isolates recovered from sample (A-B) Scotts Bluff, 2001.345 Kerr, Eric 10/26/2001 01-27 2 Isolates recovered from sample (A-B) Scotts Bluff, 2001.346 Kerr, Eric 10/26/2001 01-28 No Isolates recovered from sample Scotts Bluff, 2001.347 Kerr, Eric 10/26/2001 01-29 1 Isolates recovered from sample (A) Scotts Bluff, 2001.348 Kerr, Eric 10/26/2001 01-30 1 Isolates recovered from sample (A) Scotts Bluff, 2001.349 Kerr, Eric 10/26/2001 01-31 No Isolates recovered from sample Scotts Bluff, 2001.350 Kerr, Eric 10/26/2001 01-32 No Isolates recovered from sample Scotts Bluff, 2001.351 Kerr, Eric 10/26/2001 01-33 1 Isolates recovered from sample (A) Scotts Bluff, 2001.352 Kerr, Eric 10/26/2001 01-34 1 Isolates recovered from sample (A) Scotts Bluff, 2001.353 Kerr, Eric 10/26/2001 01-35 2 Isolates recovered from sample (A-B) Sheridan, NE 2001.332 Kerr, Eric 10/26/2001 01-14 2 Isolates recovered from sample (A-B) Sheridan, NE 2001.333 Kerr, Eric 10/26/2001 01-15 3 Isolates recovered from sample (A-C)

Sumac Natrona, WY 2001.136 Logue, Judy 9/5/2001 Premature Senescence caused by Leaf Scorch and Heat Stress

31

County/State EPPL ID CES Agent Date In Diagnosis/Recommendation Pathogen Tomato Campbell, WY 2001.118D Thompson, Janet 8/6/2001 Early Blight Alternaria solani Fremont, WY 2001.047 Cunningham, Ron 5/1/2001 Cold Induced Phosphorus Deficiency Platte, WY 2001.084 Platte CES 6/22/2001 Probable Cold/Frost Injury Washakie, WY 2001.081 Gill, Jim 6/20/2001 Possible Frost/Cold Injury and Skeletonizing Insect Symptoms

Tomato and Pepper Hot Springs, 2001.105 Langbehn, Jerry 7/19/2001 Tomato Spotted Wilt Virus (Tomato) and Sunscald on Pepper TSWV

Tomato, Potato and Park, WY 2001.127D Stevens, Bart 8/30/2001 Probable Cronic Herbicide Carry-Over from Year to Year

Turf Albany, WY 2001.054 5/16/2001 Ascochyta Leaf Blight Ascochyta sp. Albany, WY 2001.145 Panter, Karen 9/27/2001 Curvularia and Ascochyta Blights and Anthracnose Curvularia sp. Campbell, WY 2001.031 Thompson, Janet 4/10/2001 Ascochyta Leaf Blight Ascochyta sp. Campbell, WY 2001.043 Thompson, Janet 4/30/2001 Ascochyta Leaf Blight, Anthracnose and Turf Mites Ascochyta sp. Campbell, WY 2001.048 Campbell CES 5/4/2001 Exessive Thatch Buildup Campbell, WY 2001.075D Thompson, Janet 6/14/2001 Possible Stripe Smut (presumptive from digital photograph) Johnson, WY 2001.059 Horn, Blaine 5/23/2001 Ascochyta Leaf Blight, Anthracnose and Excessive Thatch Ascochyta sp. Park, WY 2001.123 Holder, Bobbie 8/10/2001 Ascochyta Leaf Blight, Anthracnose and Excessive Thatch Ascochyta sp. Platte, WY 2001.138 Mount, Dallas 9/5/2001 Melting Out and Ascochyta Leaf Blight Helminthosporium sp. Sweetwater, 2001.079 Harrington, 6/18/2001 Ascochyta Leaf Blight, Anthracnose and Brown Patch Ascochyta sp. Weston, WY 2001.033 Taylor, Bill 4/12/2001 Fusarium Crown Rot and Leptosphaerulina Leaf Blight Fusarium sp.

Unknown Tree Campbell, WY 2001.112D Thompson, Janet 7/30/2001 Fingergall/Nipple Gall (Eriophyid Mite suspected) Sweetwater 2001.125 Staskiewicz, Rick 8/15/2001 Herbicide Damage suspected But Not Found, Probable Drought

32


Walnut Hot Springs, 2001.141D Langbehn, Jerry 9/11/2001 Unable to Determine Cause of Problem

Wheat, Winter Laramie, WY 2001.071 6/12/2001 Tan Spot, No Virus Detected by UNL Lab (ELISA) Pyrenophora tritici-repentis Laramie, WY 2001.096 Roselund, Phil 7/6/2001 Stripe Rust suspected, Signs of Rust Not Detected

Willow Park, WY 2001.061 Holder, Bobbie 5/30/2001 Dothiora Canker Dothiora polyspora

Yew Natrona, WY 2001.070 Logue, Judy 6/11/2001 Exsessive Soil Moisture, Overwatering Injury

33

Table 3. EPPL field crop samples 2001 County/State EPPL ID Date In Diagnosis/Recommendation Pathogen

Alfalfa Park, WY 2001.111D 7/25/2001 Vestigial Flower and Branched Raceme (genetic abnormality)

Bean, Dry Goshen, WY 2001.117 8/1/2001 Research sample: Culture and Identify Bacterial Isolates Probable Psp and Xcp

Bean, Pinto Cultivar/Variety Maverick Park, WY 2001.137 8/9/2001 Bacterial Bean Blight Not Detected, No Fusarium Detected

Clover, Red Cultivar/Variety Plus Park, WY 2001.085 6/20/2001 Fusarium Root & Crown Rot Fusarium sp.

Corn Goshen, WY 2001.130 8/29/2001 Purple Leaf Sheath (presumptive) Various Saprophytic Fungi

Oat Laramie, WY 2001.113 7/31/2001 Ascochyta Leaf Blight Ascochyta sp.

Potato Cultivar/Variety FL1625 NEBRASKA 2001.003 1/25/2001 Late Blight NOT Detected

NEBRASKA 2001.004 1/25/2001 Late Blight NOT Detected




34

County/State EPPL ID Date In Diagnosis/Recommendation Pathogen NEBRASKA 2001.008 1/25/2001 Late Blight NOT Detected











NEBRASKA 2001.019A 1/25/2001 Late Blight NOT Detected

NEBRASKA 2001.019B 1/25/2001 Late Blight NOT Detected

NEBRASKA 2001.022 2/15/2001 Late Blight Not Detected


NEBRASKA 2001.150 7/30/2001 ELISA Virus/Bacteria Screening






35

County/State EPPL ID Date In Diagnosis/Recommendation Pathogen NEBRASKA 2001.156 7/30/2001 ELISA Virus/Bacteria Screening





















36






















37






















38

County/State EPPL ID Date In Diagnosis/Recommendation Pathogen






















39






















40






















41






















42












NEBRASKA 2001.404 11/6/2001 Late Blight NOT Detected, Fusarium sp. Isolated from tuber Fusarium sp.

NEBRASKA 2001.405 11/30/2001 Late Blight NOT Detected, water mold isolated but not pathogenic

Sugar Beet Banner, NE 2001.340 10/26/2001 01-22 No Isolates recovered from sample

Banner, NE 2001.341 10/26/2001 01-23 1 Isolates recovered from sample (A)

Box Butte, NE 2001.329 10/26/2001 01-11 2 Isolates recovered from sample (A-B)

Box Butte, NE 2001.330 10/26/2001 01-12 5 Isolates recovered from sample (A-E)

Box Butte, NE 2001.331 10/26/2001 01-13 1 Isolates recovered from sample (A)

Cheyenne, NE 2001.401 10/26/2001 01-83 No Isolates recovered from sample

COLORADO 2001.362 10/26/2001 01-44 4 Isolates recovered from sample (A-D)

COLORADO 2001.363 10/26/2001 01-45 2 Isolates recovered from sample (A-B)

43

County/State EPPL ID Date In Diagnosis/Recommendation Pathogen COLORADO 2001.364 10/26/2001 01-46 6 Isolates recovered from sample (A-F)

COLORADO 2001.365 10/26/2001 01-47 6 Isolates recovered from sample (A-F)


COLORADO 2001.367 10/26/2001 01-49 1 Isolates recovered from sample (A)

COLORADO 2001.368 10/26/2001 01-50 No Isolates recovered from sample









COLORADO 2001.377 10/26/2001 01-59 5 Isolates recovered from sample (A-E)

COLORADO 2001.378 10/26/2001 01-60 7 Isolates recovered from sample (A-G)

COLORADO 2001.379 10/26/2001 01-61 3 Isolates recovered from sample (A-C)






44

County/State EPPL ID Date In Diagnosis/Recommendation Pathogen COLORADO 2001.385 10/26/2001 01-67 4 Isolates recovered from sample (A-D)












COLORADO 2001.400 10/26/2001 01-82 8 Isolates recovered from sample (A-H)



MONTANA 2001.319 10/26/2001 01-01 7 Isolates recovered from sample (A-G)

MONTANA 2001.320 10/26/2001 01-02 6 Isolates recovered from sample (A-F)


MONTANA 2001.322 10/26/2001 01-04 5 Isolates recovered from sample (A-E)


MONTANA 2001.324 10/26/2001 01-06 3 Isolates recovered from sample (A-C)

45

County/State EPPL ID Date In Diagnosis/Recommendation Pathogen MONTANA 2001.325 10/26/2001 01-07 6 Isolates recovered from sample (A-F)

MONTANA 2001.326 10/26/2001 01-08 2 Isolates recovered from sample (A and C)

MONTANA 2001.327 10/26/2001 01-09 7 Isolates recovered from sample (A-H)



Morrill, NE 2001.334 10/26/2001 01-16 No Isolates recovered from sample

Morrill, NE 2001.335 10/26/2001 01-17 3 Isolates recovered from sample (A-C)

Morrill, NE 2001.336 10/26/2001 01-18 2 Isolates recovered from sample (A-B)


Morrill, NE 2001.338 10/26/2001 01-20 6 Isolates recovered from sample (A-F)

Morrill, NE 2001.339 10/26/2001 01-21 10 Isolates recovered from sample (A-K)

NEBRASKA 2001.355 10/26/2001 01-37 3 Isolates recovered from sample (A-C)

NEBRASKA 2001.356 10/26/2001 01-38 7 Isolates recovered from sample (A-G)

NEBRASKA 2001.357 10/26/2001 01-39 2 Isolates recovered from sample (A-B)

NEBRASKA 2001.358 10/26/2001 01-40 6 Isolates recovered from sample (A-F)






NEBRASKA 2001.390 10/26/2001 01-72 4 Isolates recovered from sample (A-D)

46

County/State EPPL ID Date In Diagnosis/Recommendation Pathogen Scotts Bluff, 2001.342 10/26/2001 01-24 5 Isolates recovered from sample (A-E)

Scotts Bluff, 2001.343 10/26/2001 01-25 1 Isolates recovered from sample (A)

Scotts Bluff, 2001.344 10/26/2001 01-26 2 Isolates recovered from sample (A-B)


Scotts Bluff, 2001.346 10/26/2001 01-28 No Isolates recovered from sample








Sheridan, NE 2001.332 10/26/2001 01-14 2 Isolates recovered from sample (A-B)

Sheridan, NE 2001.333 10/26/2001 01-15 3 Isolates recovered from sample (A-C)

Wheat, Winter Laramie, WY 2001.071 6/12/2001 Tan Spot, No Virus Detected by UNL Lab (ELISA) Pyrenophora tritici-repentis

Laramie, WY 2001.096 7/6/2001 Stripe Rust suspected, Signs of Rust Not Detected

47

Table 4. EPPL tree and shrub samples 2001 County/State EPPL ID Date In Diagnosis/Recommendation Pathogen

Apple Campbell, WY 2001.072D 6/7/2001 Poor Establishment (presumptive)

Campbell, WY 2001.078 6/18/2001 Cytospora Canker Cytospora sp.

Campbell, WY 2001.107 7/19/2001 Fire Blight Erwinia amylovora

Goshen, WY 2001.126 8/23/2001 Fire Blight suspected But Not Detected, Fruit had Insect Damage

Ash Park, WY 2001.122 8/10/2001 Ash Leafspot Phyllosticta fraxinicola

Aspen Hot Springs, 2001.099D 7/3/2001 Probable Ink Spot Damage, difficult to determine from images.

Hot Springs, 2001.119 8/6/2001 Drought Injury

Uinta, WY 2001.144 9/18/2001 Shoot Blight and Cytospora Canker (canker presumptive based on description) Venturia tremulae

Aspen, Apple and Maple Uinta, WY 2001.106 7/17/2001 Leaf Scorch and Drought Injury

Birch Cultivar/Variety Cutleaf Campbell, WY 2001.038 4/27/2001 Suspect Scale insects not found, some type of residue.

Caragana Weston, WY 2001.086 6/22/2001 Poor Establishment and Vigor

Chokecherry Platte, WY 2001.128D 8/30/2001 Peach Tree Borer and Crown Rot, presumptive based on images

48


Cottonwood and Aspen Cultivar/Variety Soiuxland (cottonwood) Campbell, WY 2001.055 5/18/2001 Cytospora Canker and Hail Damage Cytospora sp.

Elm, Chinese Niobrara, WY 2001.121 8/13/2001 Insect Damage suspected, Forwarded to Mike Brewer (Ext. Ento.)

False Astilbe Park, WY 2001.103 7/18/2001 Leaf Scorch (due to dry hot conditions) and Poor Establishment

Fir Lincoln, WY 2001.041 4/27/2001 Lichens Growing on Branch

Hedge Campbell, WY 2001.316D 10/11/2001 Leaf Scorch Associated with Heat Stress

Juniper Park, WY 2001.056 5/18/2001 Spruce Spider Mite Infestation

Uinta, WY 2001.132 8/31/2001 Cedar Apple Rust Galls Gymnosporangium sp.

Lilac Albany, WY 2001.149 10/2/2001 Powdery Mildew Microsphaera penicillata

Park, WY 2001.062 5/30/2001 Frost Damage

Park, WY 2001.090 6/29/2001 Probable Frost Injury

Platte, WY 2001.066D 6/6/2001 Frost Damage

Lilac, Spruce and Elm Albany, WY 2001.093 7/3/2001 Herbicide Injury

49


Linden, Spotted Platte, WY 2001.060D 5/29/2001 Cold Temperature or Herbicide Injury (presumptive)

Mountain Ash Campbell, WY 2001.114 7/27/2001 Drought Injury and Mite Infestation

Fremont, WY 2001.116 7/30/2001 Fire Blight Suspected but Not Detected, Mite Infestation Found

Oak Campbell, WY 2001.110D 7/24/2001 Leaf Scorch / Drought Stress

Pine Campbell, WY 2001.042 4/30/2001 Winter Injury

Campbell, WY 2001.088D 6/27/2001 Drought Injury

Campbell, WY 2001.318 10/26/2001 None - Unable to determine cause of problem

Hot Springs, 2001.148D 10/2/2001 Chronic Stress Possibly Attributed to Overwatering and Lack of Growing Space

Laramie, WY 2001.035 4/20/2001 Dothistroma pini Not Detected

Natrona, WY 2001.051 5/11/2001 Winter Injury/Dessication

Natrona. WY 2001.076 6/15/2001 Brown Felt Blight and Slime Mold Fruiting Structures Neopeckia coulteri

Niobrara, WY 2001.409 12/6/2001 Twig Beetles Found (they are still active)

Park, WY 2001.044 5/1/2001 Winter Injury/Dessication

Platte, WY 2001.089D 6/27/2001 Propable White Pine Weevil Damage (based on image and description)

Uinta, WY 2001.045 5/1/2001 White Pine Blister Rust Cronartium ribicola

Washikie, WY 2001.001 1/8/2001 Dessication/Moisture Stress n/a

50


Pine and Aspen Teton, WY 2001.109 7/23/2001 Pine Needle Scale (Pine) and Earwigs Associated with Aspen Leaves Plum Cultivar/Variety Wild Niobrara, WY 2001.069 6/11/2001 Fingergall Mites

Poplar Cultivar/Variety Northwest Crook, WY 2001.139 9/11/2001 Iron Chlorosis, Herbicide Damage Not Likely

Fremont, WY 2001.057D 5/23/2001 Frost Injury

Red Cedar Hot Springs, 2001.120 8/6/2001 Juniper Borer Damage

Spirea Johnson, WY 2001.087 6/22/2001 Probable Frost/Cold Injury

Spruce Albany, WY 2001.315 10/4/2001 Probable Moisture Stress and Fertility Problems

Big Horn, WY 2001.098D 7/6/2001 Diagnosis Not Conclusive due to Poor Image, possible Winter/Drought Injury

Campbell, WY 2001.025 3/14/2001 Insect Damage - White Pine Weevil

Campbell, WY 2001.026D 3/21/2001 Winter Injury

Campbell, WY 2001.036 4/24/2001 Winter Injury and Possible Salts Injury

Campbell, WY 2001.040 4/27/2001 Probable Loss of Vigor and Salts Problem

Campbell, WY 2001.058 5/23/2001 Winter Injury

Campbell, WY 2001.073D 6/8/2001 Planted Too Deeply and possible Physical Damage to Growing Point

51

County/State EPPL ID Date In Diagnosis/Recommendation Pathogen Campbell, WY 2001.095 7/5/2001 Unable to Determine Cause of Problem - asked for phisical sample, it was not

Campbell, WY 2001.100 7/9/2001 Overwatering and Oedema Symptoms

Campbell, WY 2001.101 7/9/2001 Drought / Heat Stress, Possible Frost Injury this Spring

Campbell, WY 2001.131 8/30/2001 Poor Vigor and Possible Salts Problem

Campbell, WY 2001.146 9/26/2001 Poor Establishment and Loss of Vigor (new planting)

Converse, WY 2001.068 6/6/2001 Probable Drought Injury / Winter Injury

Goshen, WY 2001.020 2/15/2001 Winter Injury n/a

Hot Springs, 2001.140D 9/11/2001 Problem Undetermined, Possible Related to Canker or Root Inury

Johnson, WY 2001.034 4/17/2001 Winter Injury and Possible Salts Injury

Lincoln, WY 2001.143 9/14/2001 Injury to Growing Point and Drought Stress

Niobrara, WY 2001.053 5/15/2001 Dessication/Drought Injury

Niobrara, WY 2001.147 9/27/2001 Overwatering Injury and Pine Scale

Sheridan, WY 2001.002 1/16/2001 Salts (Soil/Water) Problem; presumtive n/a

Sweetwater, 2001.063 5/31/2001 Presumtive Salts Injury

Sweetwater, 2001.064 5/31/2001 Drought Injury/Winter Injury and Pine Needle Scale Infestation

Teton, WY 2001.406 12/3/2001 Poor Vigor and Establishment Problems

Teton, WY 2001.407 12/3/2001 Transplant Shock and Poor Establishment

Uinta, WY 2001.027 4/3/2001 Winter Injury

Uinta, WY 2001.050 5/10/2001 Winter Injury/Desiccation

Spruce and Pine Cultivar/Variety Limber Albany, WY 2001.080 6/19/2001 No Disease Detected, Probably Drought/Winter Injury

52

County/State EPPL ID Date In Diagnosis/Recommendation Pathogen Campbell, WY 2001.037 4/24/2001 Winter / Desiccation Injury

Park, WY 2001.024 3/9/2001 Probable Management Problems

Park, WY 2001.052 5/11/2001 Fertility Problem and Possible Lack of Water

Park, WY 2001.065 6/1/2001 Cytospora Canker Cytospora kunzei

Park, WY 2001.077 6/18/2001 Probable Salts Injury and Poor Vigor

Park, WY 2001.092 7/2/2001 No Disease Detected - Possible Chemical or Environmental Injury

Platte, WY 2001.029 4/6/2001 Winter Injury

Platte, WY 2001.030 4/6/2001 Winter Injury

Platte, WY 2001.094 7/3/2001 Cytospora Canker of Spruce (presumptive) cankers present but spores not found Cytospora kunzei

Sumac Natrona, WY 2001.136 9/5/2001 Premature Senescence caused by Leaf Scorch and Heat Stress

Unknown Tree Campbell, WY 2001.112D 7/30/2001 Fingergall/Nipple Gall (Eriophyid Mite suspected)

Sweetwater 2001.125 8/15/2001 Herbicide Damage suspected But Not Found, Probable Drought Injury

Walnut Hot Springs, 2001.141D 9/11/2001 Unable to Determine Cause of Problem

Willow Park, WY 2001.061 5/30/2001 Dothiora Canker Dothiora polyspora

Yew Cultivar/Variety Japanese Natrona, WY 2001.070 6/11/2001 Exsessive Soil Moisture, Overwatering Injury

53

Table 5. EPPL home and garden samples 2001 County/State EPPL ID Date In Diagnosis/Recommendation Pathogen

Aster Natrona, WY 2001.115 7/30/2001 Fusarium Wilt and Yellows Fusarium sp.

Ficus Niobrara, WY 2001.032 4/10/2001 Leaf Drop Due to Management Problems

Geranium Hot Springs, 2001.082 6/19/2001 Environmental Injury suspected, Herbicide Injury Not Suspected

Hollyhock Albany, WY 2001.104 7/19/2001 Severe Mite Infestation

Albany, WY 2001.108 7/24/2001 Endophyllum Rust and Mite Infestation Endophyllum tuberculatum

Washakie, WY 2001.067 6/6/2001 Probable Iron Chlorosis and Insect Problem

Potato Campbell, WY 2001.124D 8/14/2001 Potato Leaf Roll Virus, presumptive from image PLRV

Lincoln, WY 2001.317 10/26/2001 Common Scab Streptomyces scabies

Natrona, WY 2001.129 8/30/2001 Soft Rot with Possible association with Western Leak Erwinia cartovora

Raspberry Park, WY 2001.135 9/5/2001 Yellow Rust (presuptive based on symptoms and single spore found) Phragmidium rubi-idaei

Rhubarb Sweetwater, 2001.083D 6/21/2001 Root and Crown Rot, presumptive based on symptoms

54


Tomato Campbell, WY 2001.118D 8/6/2001 Early Blight Alternaria solani

Platte, WY 2001.084 6/22/2001 Probable Cold/Frost Injury

Washakie, WY 2001.081 6/20/2001 Possible Frost/Cold Injury and Skeletonizing Insect Symptoms

Tomato, Potato and Park, WY 2001.127D 8/30/2001 Probable Chronic Herbicide Carry-Over from Year to Year

55

Table 6. EPPL turf samples 2001 County/State EPPL ID Date In Diagnosis/Recommendation Pathogen(s)

Turf Albany, WY 2001.054 5/16/2001 Ascochyta Leaf Blight Ascochyta sp.

Albany, WY 2001.145 9/27/2001 Curvularia and Ascochyta Blights and Anthracnose Curvularia sp. Ascochyta sp.

Campbell, WY 2001.031 4/10/2001 Ascochyta Leaf Blight Ascochyta sp.

Campbell, WY 2001.043 4/30/2001 Ascochyta Leaf Blight, Anthracnose and Turf Mites Ascochyta sp. Colletotrichum graminicola

Campbell, WY 2001.048 5/4/2001 Exessive Thatch Buildup

Campbell, WY 2001.075D 6/14/2001 Possible Stripe Smut (presumptive from digital photograph)

Johnson, WY 2001.059 5/23/2001 Ascochyta Leaf Blight, Anthracnose and Excessive Thatch Ascochyta sp. Colletotrichum graminicola

Park, WY 2001.123 8/10/2001 Ascochyta Leaf Blight, Anthracnose and Excessive Thatch Ascochyta sp. Colletotrichum graminicola

Platte, WY 2001.138 9/5/2001 Melting Out and Ascochyta Leaf Blight Helminthosporium sp. Ascochyta sp.

Sweetwater, 2001.079 6/18/2001 Ascochyta Leaf Blight, Anthracnose and Brown Patch Ascochyta sp. Colletotrichum graminicola

Weston, WY 2001.033 4/12/2001 Fusarium Crown Rot and Leptosphaerulina Leaf Blight Fusarium sp. Leptosphaerulina trifolii

56

Table 7. EPPL commercial horticulture samples 2001 County/State EPPL ID Date In Diagnosis/Recommendation Pathogen

Basil Sheridan, WY 2001.102 7/11/2001 Severe Thrips Infestation

Pepper, Sweet Bell Fremont, WY 2001.046 5/1/2001 No Disease Detected; Probable Sulfur Dioxide/Ethylene Damage

Petunia Cultivar/Variety White Madona Goshen, WY 2001.028 4/6/2001 No Pathogens Detected - Probable Abiotic Problem

Tomato Cultivar/Variety Early Girl Fremont, WY 2001.047 5/1/2001 Cold Induced Phosphorus Deficiency

Tomato and Pepper Cultivar/Variety Burpees Better Boy (Tomato) Hot Springs, 2001.105 7/19/2001 Tomato Spotted Wilt Virus (Tomato) and Sunscald on Pepper TSWV

57

Table 8. EPPL non-plant and plant identification samples 2001 County/State EPPL ID Date In Diagnosis/Recommendation Problem ID

Fungal Growth Platte, WY 2001.097D 7/6/2001 Fungal Growth In Wet Arena Soil/Manure on Floor , Saprophytic Growth

Mold Identification Campbell, WY 2001.142 9/14/2001 Penicillium sp. Penicillium sp.

Moldy Dry Wall Sample Campbell, WY 2001.039 4/27/2001 Alternaria and Ulocladium spp. Fungi Recovered Alternaria sp.

Mushroom ID Campbell, WY 2001.074D 6/13/2001 Type of Russula Mushroom (presumptive ID from photograph)

Organic Debris Natrona, WY 2001.134 9/4/2001 Sample Contained Decayed/Composted Plant Material

Plant ID Park, WY 2001.133 8/20/2001 Narrowleaf Cottonwood, Populus angustifolia

Sweetwater, 2001.091 7/2/2001 Western Peppergrass, Lepidium montana

58

Table 9. EPPL high plains regional Cercospora survey 2001 Cercospora Leaf Spot Survey (sample numbers 20001.319 through 2001.403) A survey is being conducted in Wyoming and neighboring states to determine fungicide sensitivity of the fungus Cercospora beticola, the causal agent of Cercospora leaf spot of sugar beet. This disease is causing economic loss in the High Plains. Therefore, surveys include infected sugar beet recovered from growers’ fields in Colorado, Montana, Nebraska, and Wyoming. Diagnostic results are being used to limit fungicide usage to those products that are most effective in each particular production area. Samples submitted during 2001 for this survey are listed as numbers 2001.319 through 2001.403. For additional information on survey results, Gary Franc, at (307) 766-2397 ([email protected]). Previous survey results were published in the Journal of Sugar Beet Research (Fungicide sensitivity characteristics of Cercospora beticola isolates recovered from the High Plains of Colorado, Montana, Nebraska, and Wyoming. 1. Benzimidazole and Triphenyltin Hydroxide. S. C. Briere, G. D. Franc, and E. D. Kerr. J. Sugar Beet Res. vol 38:111-120).

County/State EPPL ID Date In Sample ID and Number of Isolates Recovered

Banner, NE 2001.340 10/26/2001 01-22 No Isolates recovered from sample

Banner, NE 2001.341 10/26/2001 01-23 1 Isolates recovered from sample (A)

Box Butte, NE 2001.329 10/26/2001 01-11 2 Isolates recovered from sample (A-B)

Box Butte, NE 2001.330 10/26/2001 01-12 5 Isolates recovered from sample (A-E)

Box Butte, NE 2001.331 10/26/2001 01-13 1 Isolates recovered from sample (A)

Cheyenne, NE 2001.401 10/26/2001 01-83 No Isolates recovered from sample















COLORADO 2001.376 10/26/2001 01-58 2 Isolates recovered from sample (A-B) County/State EPPL ID Date In Sample ID and Number of Isolates Recovered

59


COLORADO 2001.378 10/26/2001 01-60 7 Isolates recovered from sample (A-G)



















COLORADO 2001.400 10/26/2001 01-82 8 Isolates recovered from sample (A-H)








MONTANA 2001.324 10/26/2001 01-06 3 Isolates recovered from sample (A-C)


MONTANA 2001.326 10/26/2001 01-08 2 Isolates recovered from sample (A and C)

MONTANA 2001.327 10/26/2001 01-09 7 Isolates recovered from sample (A-H) County/State EPPL ID Date In Sample ID and Number of Isolates Recovered

60




Morrill, NE 2001.335 10/26/2001 01-17 3 Isolates recovered from sample (A-C)




Morrill, NE 2001.338 10/26/2001 01-20 6 Isolates recovered from sample (A-F)

Morrill, NE 2001.339 10/26/2001 01-21 10 Isolates recovered from sample (A-K)


NEBRASKA 2001.356 10/26/2001 01-38 7 Isolates recovered from sample (A-G)








NEBRASKA 2001.390 10/26/2001 01-72 4 Isolates recovered from sample (A-D)

Scotts Bluff, 2001.342 10/26/2001 01-24 5 Isolates recovered from sample (A-E)












Sheridan, NE 2001.332 10/26/2001 01-14 2 Isolates recovered from sample (A-B) County/State EPPL ID Date In Sample ID and Number of Isolates Recovered

61

Sheridan, NE 2001.333 10/26/2001 01-15 3 Isolates recovered from sample (A-C)

62

Table 10. EPPL potato diseases testing for the Potato Certification Association of Nebraska 2001 PCAN is the agency that certifies the Wyoming and Nebraska potato seed crop. Virus and bacterial testing in support of this program was conducted during 2001 as samples 2001.150 through 2001.314. For additional information on testing results, contact the extension plant pathology specialist at (307) 766-2397 ([email protected]).

County/State EPPL ID Date In Diagnosis/Tests Preformed

















NEBRASKA 2001.019A 1/25/2001 Late Blight NOT Detected

NEBRASKA 2001.019B 1/25/2001 Late Blight NOT Detected










63

County/State EPPL ID Date In Diagnosis/Tests Preformed NEBRASKA 2001.157 7/30/2001 ELISA Virus/Bacteria Screening































64

































65

































66


































67






























68

EPPL 2001 Report Survey Questionnaire We are always striving to provide the best service to our EPPL clientele. We would appreciate any comments or suggestions that would help us improve this report in the next revision. Use the space provided below to write your comments. Please photocopy this page and mail it to the address shown below. Thank you. Please mail to: Attention: Extension Plant Pathology Laboratory University of Wyoming Plant Sciences Department P.O. Box 3354 Laramie, WY 82071-3354

Documents

EPPL 2001 report - wyomingextension.org · Laboratory (EPPL).....4 Table 1. EPPL total number of samples submitted from 1991-2001.....5 Table 2. EPPL diagnostic samples for 2001.....14