Epidemiology & Public Health Unit (epi@ipc) 2015 · Unit (epi@ipc) 2015 The scientific & activity report of the Epidemiology & Public Health Unit of the Institut Pasteur du Cambodge

Epidemiology & Public Health Unit (epi@ipc) 2015The scientific & activity report of the Epidemiology & Public Health Unit of the Institut Pasteur du Cambodge (epi@ipc) for the period January 1st to December 31st, 2015.

Scientific & activity report

epi@ipc 2015 Scientific & activity Report

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Contents

Team members and functional structure of the Unit.............................................................................................. 4 Timeline and significant events in 2015 .................................................................................................................. 5

Students and training in the Unit ............................................................................................................................... 5

Students ..................................................................................................................................................................... 5

Participation in training others ............................................................................................................................. 5

AET and UHS training ........................................................................................................................................ 5

International Pasteur course on Rabies Surveillance and Control ............................................................. 5

OpenClinica training, IPC June 2015 ............................................................................................................. 5

SEAe training ....................................................................................................................................................... 5

Training ourselves .................................................................................................................................................... 5

Networking and epi@ipc .......................................................................................................................................... 8

Partners in Cambodia ............................................................................................................................................ 8

Hospitals ............................................................................................................................................................... 8

Institutions ............................................................................................................................................................. 8

Partners within IPIN ................................................................................................................................................. 8

Regional or International Partnerships ................................................................................................................ 8

Scientific projects and advancement .................................................................................................................... 10

Endemic ID (Dengue, influenza, CAP…) .......................................................................................................... 10

DENFREE ............................................................................................................................................................ 10

DVI study ........................................................................................................................................................... 10

IMMI ................................................................................................................................................................... 11

Clinical research ................................................................................................................................................... 11

STATIS ANRS 12290 ...................................................................................................................................... 11

BIRDY ................................................................................................................................................................. 11

ECOMORE Myanmar ...................................................................................................................................... 12

Emerging ID / zoonoses : A(H5N1), rabies … ............................................................................................... 12

A(H5N1) ............................................................................................................................................................ 12

Rabies ................................................................................................................................................................ 13

Sick dogs exposure lookback and immune response study (RESIST)-I .................................................. 13

Other .................................................................................................................................................................. 13

South East Asia encephalitis project (SEAe) .................................................................................................... 14

Cambodia ......................................................................................................................................................... 14

Prospective: flagship projects to be implemented in 2016 ............................................................................. 15

Rabies PEP immune response study (RESIST) II ........................................................................................... 16


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Vaccine Match to Pneumococcal Strains (ViMPS) ...................................................................................... 16

Pertussis Immunization programmes in Low Income Countries (PERILIC) ................................................ 16

Tenofovir As PRevention Of Hepatitis b transmission for Mothers (TA PROHM - ANRS 12345) .... 17

Animal studies on zoonoses ............................................................................................................................ 17

Development of a Lao PDR-Cambodia One Health Surveillance and Laboratory Network (LACANET) ......................................................................................................................................................... 18

PAthogens’ NIChe: a new approach for infectious diseases control (PANIC) ...................................... 18

Support to National authorities ............................................................................................................................. 18

Scientific communications ......................................................................................................................................... 20

Peer-reviewed articles published ..................................................................................................................... 20

Accepted for publication .................................................................................................................................... 21

Abstracts presented at conferences and meetings ........................................................................................ 21

Other ...................................................................................................................................................................... 22

Annex: Impact Factor for 26/29 published or accepted epi@ipc articles (by first author) published or accepted in 2015* ...................................................................................................................... 23

Annex: ERA 2010 scores for 20/29 rated and published or accepted epi@ipc articles published or accepted, 2015 * ........................................................................................................................ 23

Annex: Available altmetrics for 8 articles in Plos or BMC journals (to 10 Jan. 2015) ............................... 24

Annex: Ongoing joint projects between epi@ipc and other IPC units, Dec. 2015. .................................... 25

Annex: Transferring IPC research findings into policy ...................................................................................... 26

Report prepared by Arnaud TARANTOLA based on material developed by the epi@ipc team. Arnaud Tarantola, MD, Msc Head, Epidemiology and Public Health Unit http://www.pasteur-kh.org/research/epidemiology/ Institut Pasteur du Cambodge 5, Bvd. Monivong BP 983 - Phnom Penh Royaume du Cambodge Email: [email protected]


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eam members and functional structure of the Unit Below is a schematic representation of functional structure of research at the Epidemiology & Public Health unit, Institut Pasteur du Cambodge (epi@ipc), 2011-2015. Apart from the Vaccinations unit (10 staff), epi@ipc has 30+ staff doing groundbreaking research on major issues: HIV/AIDS, TB, influenza, dengue, rabies or A(H5N1). The unit is a recognized center for

clinical research in Asia.

The diverse and state-of-the-art activity1 in epi@ipc is conducted by Cambodian and international epidemiology personnel with varied backgrounds in Medicine, Pharmacy, Veterinary medicine or social sciences. These personnel are organized in several groups, a given person sometimes contributing to several teams depending on his or her competence and availability and the team’s needs. This variable-geometry and matrix organization implemented is represented in Figures 1 and 2.

Figure 1: epi@ipc staff members in 2015

Head of Epidemiology and Public Health Unit: Arnaud Tarantola ; Admin Assistant Ms. Phunlang Sun.

Legend Group leader Permanent team members

Endemic diseases Sowath Ly Nguon Kunthy ; Chan Siam; Sopheak Sorn ; Souv Kimsan; Saman Manil; Chanthy leng; Pak So Kreth; Camille Fortas; Arnaud Tarantola

Clinical Research

Laurence Borand

Laurence Borand, Dim Bunnet; Sophea Suom; Sophie Goyet; Keo Monorea; Manil Saman; Chanthy Leng; Vorn Rany; Susan Ramsey; Nour Shamas; Thann Sreymom; Chon Thida; Meng Vatana; Agathe de Lauzanne, Véronique Ngo, Sorunna Born, Ku Thry, Sitha Sin, Sophal Ouch,Veasna Hak, Channimol Sarith, Sovanda Muth, Siyin Lach, Sok Keng Heng, Nam Sokhol, Chhon Chhouk, Nhean Kanhchana; Arnaud Tarantola

Emerging infectious diseases / zoonoses

Arnaud Tarantola

Ly Sowath; Julien Cappelle; Thavry Hoem ; Laurence Borand ; Malen;; Peas Muslim; Yoann Crabol; Julia Ledien; Jean-David Pommier; Raphaël Duboz; Malin Inghammar; Arnaud Tarantola

Support to authorities Arnaud Tarantola

Sophie Goyet ; Ly Sowath, Souv Kimsan (bulletin) ; otherwise ad hoc

PhD student: Arnaud Tarantola

ANRS representative: Hubert Barennes

CIRAD guest researchers: Julien Cappelle; Raphaël Duboz

Figure 2: A functional diagram of the epi@ipc activities and collaborations in 2014.

1 Brownson RC et al. Charting a future for epidemiologic training. Annals of Epidemiology [2015, 25(6):458-465]

Unit Themes Studies / Projects Other IPCDENFREE ; DVI ; PANIC Viro

ECOMORE (with Chikungunya) Viro

Influenza IMMI case-control Viro/LABM

Paanther 01 ANRS 12 229 Viro/LABM

STATIS ANRS 12 290 Viro/LABM

AMR BIRDY LABM

Viro

Leptospirosis Burden eco-epidemiological study LABM

A(H5N1) Contact tracing; Market study; Genetics Viro

Encephalitis SEA encephalitis ; Ev71; Nipah; Bat ecology Viro

Antibiotics AGISAR LABM

Applied epi AET ; outbreaks Viro / LABM

Infectious diseases Viro

Zoonoses Viro

Dengue weekly bulletin Viro

epi@

ipc

Zoonoses and emerging risks

Programs

Rabies lookback ; dog survey ; economic studies

Assisting Cambodian authorities

Expert groups

Endemic diseases Dengue

Clinical research

Rabies

HIV/TB

T

epi@ipc 2015 Scientific activity Report

imeline and significant events in 2015 Year 2015 was a year of stabilization at epi@ipc: end of the pilot phases of SEAe and

BIRDY; end of the PAANTHER ANRS 12229 data collection; continuation of the STATIS ANRS 12290 and RESIST studies...

Some permanent staff members left the team (Mousumi RAHMAN, PAK So Kreth, CHAN Malen) while others joined the team (Julia LEDIEN; PEAS Muslim; VORN Rany; THANN Sreymom; CHON Thida; MENG Vatana in December).

tudents and training in the Unit The unit has a mandate to undertake training in and outside IPC, constantly improving staffs’ skills and helping in the

ongoing development of Cambodia and the Region through capacity building.

Students Six Masters students (including two from Cambodia) undertook internships in the unit, along with veterinarian and medical students. Students and training programs are listed in Table 1 below.

Participation in training others The epi@ipc team was involved in or conducted several trainings, workshops or lectures.

AET and UHS training Dr. LY Sowath delivered two lectures to the MoH Applied Epidemiology Training epidemiology students as well as one seminar session on scientific research for 5th-year UHS medical students

International Pasteur course on Rabies Surveillance and Control

Data, expertise and experience from Cambodia were shared with an international audience during an 11-day residential course co-organized by IP Paris, and IPC in Phnom Penh, Cambodia, from October 27th to November 7th, 2015, with support from the World Health Organization and EU FP7. The purpose of this course was to provide a practical training on rabies with a special focus on Asia for students and professionals of animal and human public health sectors. The course emphasized the need of multidisciplinary approach and intersectoral cooperation. The course was attended by 20 students from 10 Asian countries (Armenia, Cambodia, China, India, Iran, Lao PDR; Pakistan, Philippines, Sri Lanka and Vietnam), including four students from Cambodia. Students were

veterinarians, physicians and microbiologists working in ministries, regional veterinary stations, International health Organization (WHO regional and country offices), hospitals, research institutions and NGOs involved in public health. The training drew on the experience and expertise of 25 teachers originating from seven countries (from United Kingdom, South Africa, Cambodia, Switzerland, Italy, Bangladesh, Thailand, Philippines and France) on three continents. Lab training took place at IPC. A field mission was conducted in Trapeang Roka village, Kampong Speu province.

OpenClinica training, IPC June 2015 Thanks to ECOMORE support, an epi@ipc training course on discovery, use and design of clinical trial databases with OpenClinica was held in Phnom Penh at IPC from 22 to 26 June 2015.

SEAe training Drs. Yoann CRABOL then Jean-David POMMIER undertook training seminars in clinical neurology in Kantha Bopha (Phnom Penh), NPH (Hanoi) and Mahosot (Vientiane) hospitals as part of SEAe.

Training ourselves The table below lists epi@ipc staff who received training or self-trained in 2015, including in the seminars and courses outlined above. In-house academic training is a challenge in an Epidemiology and Public Health Unit with 30+ staff of diverse levels, missions and areas of expertise. A strategic choice was therefore made to promote self-training through cost-free web-based educational offers. All epi@ipc staff member has received CITI certification.

T

SA dog on the main road, Trapeang Roka, Nov. 2015 (at@ipc)


Table 1: Students who received training at or in connection with epi@ipc in 2015.

Name Degree Institution Time period

Subject

Nicolas BOSC Medical student

Université Paris-Nord

July 2015 Review of validated tools to assess psychological status in pediatric and adult populations in Southeast Asia.

Agathe DE LAUZANNE MPH Ecole Pasteur-CNAM

June-Oct 2015

Non-tuberculosis mycobacteria in HIV/AIDS patients

Juliette GAMBARETTI Engineer Agro Paris Tech Sept 2015 – March 2016

A(H5N1) circulation in poultry markets in Cambodia

Caroline THEOULE Pharmacie Université Lyon 1 Oct – Dec 2015

Nutritional supplementation using Spiruline

Juliette Di FRANCESCO MPH Ecole Pasteur-CNAM

Jun-Nov 2015

JEV dynamics in pig herds

Camille FORTAS MPH EHESP Feb-Jun 2015

Risk factors associated with DENV infection in Kg Cham 2012-2013

Sébastien GARCIA Veterinarian Véto Toulouse Jun-Aug 2015

Ecological traits of bats

Clément DUSSOT Veterinarian Véto Toulouse Jun-Aug 2015

Maëlle FUSILLIER Veterinarian Véto Toulouse Jun-Dec 2015

Modelling of Nipah virus circulation in a colony of Pteropus lylei

PENG Borin Biodiversity & Conserv. Master

Royal University of Phnom Penh

May 2015 – Dec 2016

Diversity and population dynamics of the vectors of JE in rural and periurban Cambodia

Jessy NGAMI MPH Université Caen Jan-Jun 2015

Spatial and temporal dynamics of JEV circulation in humans in Cambodia

KONG Lida Bachelor of Veterinary Science

Royal University of Agriculture

May 2014 – Sept. 2015

Circulation of JEV in periurban sentinel pigs

PRING Long Bachelor of Veterinary Science

Royal University of Agriculture

May 2014 – Sept. 2015

Vectors of JEV in periurban sentinel pigs

Table 2: University courses and workshops to which epi@ipc staff contributed (presentations…)

Name Degree/Workshop Institution Date Subject S. LY AET MoH /WHO Jan 2015 Chikungunya Kg Speu S. LY AET MoH /WHO July 2015 Scientific writing S. LY Seminar on research UHS May 2015 Presentation on applied epi

A. TARANTOLA Developing Novel Strategies Workshop

NIH 16-17/06/2015 SMS surveillance tools for pharmacovigilance

A. TARANTOLA M2 One-Health Université Nantes 04/03/2015

Rabies and rabies control in a developing setting

A. TARANTOLA International course on rabies

Pasteur – U. Lausanne Oct-Nov 2015 Rabies and rabies control in a

developing setting

S. LY International course on rabies

Pasteur – U. Lausanne Oct-Nov 2015 Rabies and rabies control in a

developing setting


Table 3: Continuing training for epi@ipc staff in 2015

Name Training subject

Course name / Institution Dates

PHOEUN Chandara Vaccinology Vaccinology/Institut Pasteur and Cnam Sept. 2015 PENG Yiksin Vaccinology Vaccinology/Institut Pasteur and Cnam Sept. 2015 KEO Monorea Vaccinology Vaccinology/Institut Pasteur and Cnam Sept. 2015 Arnaud TARANTOLA Virology 11th Pasteur-HKU regional course July 2015 CHAN Siam English Australian Centre for Education (ACE) 2015 HOEM Thavry English Australian Centre for Education (ACE) 2015 SORN Sopheak English Australian Centre for Education (ACE) 2015 NGUON Kunthy English Australian Centre for Education (ACE) 2015 KEO Monorea English Australian Centre for Education (ACE) 2015 Laurence BORAND e-databases OpenClinica / epi@ipc June 2015 Nour SHAMAS e-databases OpenClinica / epi@ipc June 2015 PAK So Kreth e-databases OpenClinica / epi@ipc June 2015 SOUV Kimsan e-databases OpenClinica / epi@ipc June 2015 LY Sowath e-databases OpenClinica / epi@ipc June 2015 SUOM Sophea e-databases OpenClinica / epi@ipc June 2015 SUOM Sophea English Australian Centre for Education (ACE) July-Sept. 2015 KEO Monorea GCP CITI Program online training Dec 2015 KEO Monorea Tuberculosis Online course / The Union Aug. 2015 SORN Sopheak GIS Health GIS and Risk mapping session 1 Nov 2015 HOEM Thavry GIS Health GIS and Risk mapping session 1 Nov 2015 HOEM Thavry Epi Participatory Epidemiology training course Feb 2015 SORN Sopheak GCP CITI Program online training Dec 2015 LY Sowath GCP CITI Program online training Dec 2015 LACH Siyin Nutrition MSc of Nutrition / NIPH Ongoing PEAS Muslim Public Health MPH / NIPH Ongoing PEAS Muslim GCP CITI Program online training Dec 2015 SUOM Sophea HIV HIVNAT Bangkok Jan 2015 KEO Monorea HIV HIVNAT Bangkok Jan 2015 DIM Bunnet HIV HIVNAT Bangkok Jan 2015 HOEM Thavry GIS Health GIS and Risk mapping session 1 Nov 2015 HOEM Thavry English Australian Centre for Education (ACE) 2015

HOEM Thavry Participatory Epidemiology

Participatory Epidemiology Practitioner training course Feb 2015

Malin INGHAMMAR Epi Epidemiology and control infectious diseases in developing countries / LSHTM

Ongoing

Figure 3: The epi@ipc team in May 2015


etworking and epi@ipc As an epidemiology unit working in the field to conduct research, epi@ipc has developed many strong ties with national and

international partners.

Partners in Cambodia Links with collaborating partners in Cambodia were further reinforced in 2015.

Hospitals The full implementation of the STATIS study following GCP, informed consent process and clinical trial management training reinforced close cooperation with teams at Sihanouk Hospital Center of Hope, Phnom Penh, and with 11 hospitals or OI/ART sites in the Phnom Penh area. The BIRDY study also gave the opportunity to build strong ties to Khmer-Soviet Friendship Hospital (KSFH) and to health centers in Kampong Speu. The SEAe study is ongoing with the Kantha Bopha foundation hospitals. The DVI dengue burden study is conducted with four provincial or district referral hospitals (Kompong Cham hospital, Prey Chhor hospital, Kampot hospital and Chhouk hospital). The ECOMORE project continues in cooperation with clinical teams from eight hospitals in Cambodia. Several workshops and trainings further reinforced ties with clinicians and PHD staff as well as CNM.

Institutions

National Most projects and studies involve the Department of Communicable Disease Control of the Ministry of Health (CDC-MoH) (rabies projects…), the Centre National de Malariology (CNM) (DVI) or both (ECOMORE). Cooperation with NCHADS and CENAT has continued through the STATIS study. World Rabies Day was an opportunity to establish ties with the Health department of the Ministry of Education.

Through his continuing work on the ecology of fruit bats in Cambodia, J. CAPPELLE has deepened ties between the Unit, the Department of Forestry at the Ministry of Agriculture and the Centre for Biodiversity

Conservation, Royal University of Phnom Penh.

Mobitel, the only fully national Cambodian mobile phone services provider has agreed to share data on mobile phone use in Cambodia, as part of the ECOMORE study.

International Namru-2 contributed to the ECOMORE entomological study and has agreed to contribute samples to a study on Streptococcus pneumoniae.

Partners within IPIN Collaborations with S. CAUCHEMEZ at the Analysis and Modeling of Epidemic Dynamics Unit, IP Paris, contributed highly to two publications (one accepted on Ev71, on in final acceptance stage on Leptospirosis). S. CAUCHEMEZ is now implicated in ECOMORE and further publications are anticipated.

A regional training course on rabies was conducted in collaboration with the Lyssavirus Dynamics and Host Adaptation Unit, National Reference Centre for Rabies, WHO Collaborating Centre for Reference and Research on Rabies, IP Paris. This cooperation will also translate into the "Rabies elimination support through integrative science and salvage therapy (RESIST)" rabies research program, as well as several publications.

Epi@ipc is participating to two studies funded by TOTAL Foundation: the PERILIC study with the Department of Infection & Epidemiology Whooping Cough and other Bordetelloses Whooping Cough and other Bordetelloses Reference Center, and the Ev71 study with IP Shanghai.

As an epidemiologist who worked on Dengue at IPC over the past years A. TARANTOLA is a member of the Scientific Advisory Board of the Pasteur Dengue Taskforce which met in October 2015. This group aims to identify collaborative research projects to answer research gaps.

Regional or International Partnerships The regional partnerships were further strengthened through clinical research projects (STATIS) and the ECOMORE, SEAe and Comacross networks. Epi@ipc researchers are in charge of the Cambodia arm of an international study on Dengue sponsored by DVI while the BIRDY project involves Cambodia and African countries.

Epi@ipc also participated to an NIH-sponsored meeting on new approaches for treating tuberculosis, liaising with stakeholders in the US, Vietnam and Singapore. Genetic studies are being conducted with Oxford teams.

N


Figure 4: epi@ipc long-term partnerships and shorter-term collaborations, 2014-2015

Figure 5: geographical locations of sites for studies implicating epi@ipc, 2014-2015

Sites where data with or without samples were collected for studies conducted by or with epi@ipc 2014-2015 Cambodia

− Battambang − Phnom Penh − Kg Cham − Kg Chhnang − Kg Speu − Kampot − Kratie − Siem Reap − Takeo

Lao PDR - Vientiane Myanmar - Yangon Vietnam

− Hanoi − Ho Chi Minh


cientific projects and advancement Projects were initiated in 2015 or continued from 2014. These projects and activities are in the line of the strategy defined for epi@ipc and described in

Figure 2.

With the exception of some One-Health projects exploring pathogens in animals or insects, all epi@ipc projects follow the same paradigm: They begin with the patient, whether recruited by surveillance, clinical research, prospective studies or outbreak investigations. They also end with the patient, with a willful effort to provide data or recommendations to inform patient management or public health response (see Annex). Studies must also provide relevant results for and from Cambodia: no studies are conducted with are not relevant both to Cambodia and beyond. Studies are conducted in Cambodia and capacitate IPC teams.

After developing protocols and SOPs, the first stage of these projects is based on identifying and screening patients in strict observance of protocol Quality data and samples are obtained after receiving informed consent from patients. These data, GPS points and other relevant information are entered in databases. The second stage is the biostatistical analysis: univariate description; bivariate and multivariate analyses of these data, usually after integrating laboratory or other diagnostic data. Thirdly, data may be integrated in models on-site or with partners abroad, along with explanatory variables (meteorological, populational, mobility, interventions…).

Endemic ID (Dengue, influenza, CAP…)

DENFREE

Epi@ipc team leaders Ly S, A. Tarantola

Objective and summary of results The data collection phase of the DENFREE study was completed in 2014. Epi@ipc has been responsible for finalizing the database and is interfacing with modeling experts to answer specific research questions at varying geographical scales. In 2015, analysis by a Masters student (Ms. Camille FORTAS) of DENFREE data from household and neighborhood investigations showed that: 1/ there is no factor or cluster effect associated with asymptomatic infection; and 2/ that people of lower socio-economic status seem at higher risk of infection; and 3/ that 40% of

the force of infection could be explained by living under the same roof as a case. Aside from suggesting that teaching families to place febrile children under bed nets, research questions remain as to where other DENV infections are acquired. Could schools be involved?

Financial support Funded by EU-FP7.

DVI study

Epi@ipc team leaders Ly S

Objective and summary of results Conducted in close cooperation with CNM, the two-year DVI project aims to measure the mortality, morbidity as well as financial burden due to Dengue in the Cambodian rural setting. This will serve as a baseline for future studies.

The initial National Ethics Committee on Human Research approval #043 NECHR was granted on 20/04/2015.

Six surveys are planned for this study in total. Three serological Surveys were conducted since June 2015. The initial baseline survey showed that 32% of 2088 participants were positive for IgG antibody against DENV.

A Health Care Utilization cross sectional survey was completed; 400 head of households were interviewed.

The Passive Hospital Surveillance has identified 49 dengue cases confirmed by NS1 rapid test or PCR since July 2015; 20 of these had positive NS1 rapid test and were included in the Cost of Illness Study that aims to include in total 200 dengue cases over two dengue seasons.

Symptomatics

Passive surveillance in 4 referral hospitals

True incidence

Repeat serosurveys in village cohorts

Detection rate

Cross sectional Health Care Utilization

survey

Cost of illness

Health economicsstudy for confirmed

dengue

DENGUEBURDENSTUDY

S


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IMMI

Epi@ipc team leaders M. Saman, A. Tarantola

Objective and summary of results The IMMI prospective case-control study on the epidemiological, clinical, bacteriological, virological and immunological determinants of influenza severity in developing countries was completed in July 2015.

In Cambodia, The first National Ethics Committee on Human Research approval #112 NECHR was granted on 13/07/2011. The first patient was enrolled on September 21st, 2011. From September 2011 to July 2015, outpatient wards of two sites (Calmette and Takeo hospitals) included 1009 eligible “control” patients. Of these, 186 (18.4%) were laboratory confirmed influenza A/B infection. During the same period, 399 admitted “cases” were eligible. A total of 72 severe cases and 186 nonsevere controls were included. Samples from 258 patients were biobanked. Data will also serve in a spinoff study on the host genetic factors associated with severity.

Financial support Funded by Institut de Microbiologie et des Maladies Infectieuses (IMMI).

Clinical research

STATIS ANRS 12290

Epi@ipc team leaders L. Borand

Objective and summary of results Despite the initiation of HAART, many patients die of tuberculosis within the first month of treatment. The STATIS (Systematic vs. Test-guided Anti-tuberculosis Treatment Impact in Severely immuno-suppressed HIV-infected adults initiating antiretroviral therapy with CD4 cell counts <100/mm3) is a randomized controlled trial aiming to compare two experimental strategies to

reduce the mortality and the occurrence of severe bacterial infection (incl. TB) at 6 months in severely immunodeficient adults infected with HIV (CD4 < 100/mm3): 1/ a strategy for intensive screening and repeated tuberculosis through workable tests during the day (Xpert MTB / RIF, LAM urinary, chest radiography); and, 2/ a strategy of systematic empirical anti -tuberculosis treatment initiated two weeks before the start of HAART.

STATIS will also compare the occurrence of severe illness (AIDS, severe non-AIDS diseases, IRIS, adverse events grade 3 or 4), the number of lost to follow-up, healthcare costs, immuno- virological efficacy of ARVs , the efficacy and tolerance of TB and drug resistance and the cost-effectiveness study strategies.

The initial National Ethics Committee on Human Research approval #0195 NECHR was granted on 30/06/2014. A total of 90 patients were included in the study to 31st Dec. 2015 in this multi-country trial.

Financial support ANRS

BIRDY

Epi@ipc team leaders S. Goyet; Agathe de Lauzanne; L. Borand

Objective and summary of results The Birdy (Bacterial Infections and antibiotic-Resistant Diseases among Young children in low-income countries) study is a multi-country study which began in Madagascar in Sept 2012, with more than 800 subjects included to date. The aim of this prospective study is to estimate the incidence of bacterial infections and of their antimicrobial resistance to antibiotics: neonatal infections (<28 days of

life), infections in infants and young children (28 days to <2 years). Secondary aims are : To estimate the incidence of bacterial neonatal infections (both sensitive and resistant to antimicrobials); to describe the bacterial etiologies and severity of neonatal infections in LICs ; To assess the

A mother and her newborn included in the BIRDY study


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vertical transmission of multi-resistant bacteria from mother-to-child, versus infections contracted via the environment and from relatives; To analyze the medical and economic burden of antimicrobial resistance, in view of the available treatments and current therapeutic recommendations in Cambodia.

The initial National Ethics Committee on Human Research approval #108 NECHR was granted on 29/04/2014. In Cambodia a pilot phase was first implemented in 2014 and was scaled up in 2015 in two communities, one rural and one urban. To December 31st, 2015, a total of 481 women included in the study have given birth to 404 newborns (1 stillbirth and 6 deaths).

Financial support Funded by IP Paris, Principality of Monaco, Total Foundation and MSD Avenir.

ECOMORE Myanmar

Epi@ipc team leaders A. Tarantola

Objective and summary of results At the request of the Minister of Health, IPC teams (team leader: virology unit) set up a system to diagnose and monitor causes of severe acute respiratory infections (SARI) in pediatric wards.

The project is capacitating the National Health Laboratory for the diagnosis of viral and bacteriological pathogens of public health interest. This improved/strengthened competence immediately benefits individual and public health by being applied to SARI which are major causes of morbidity and mortality, particularly in children. To date, over 300 cases have been clinically and biologically documented. Bronchial aspiration has also been introduced in participating hospitals. Further collaboration will be undertaken to discuss patient files and attribute causality to identified pathogens. This system will allow early detection of new / emerging pathogens or outbreaks related to SARI in Yangon, including potential public health events of international concern (PHEIC) which are notifiable under the Revised IHR2.

2 A 5-year memorandum of understanding was signed between The Institut Pasteur International Network on September 13, 2012 to contribute to the sustainable improvement of laboratory services and networks,

Financial support Agence Française de Développement

Emerging ID / zoonoses: A(H5N1), rabies …

A(H5N1)

Epi@ipc team leaders S. Ly and A. Tarantola

Market studies In 2015, a prospective 12-months cohort study poultry workers at baseline and follow-up was be implemented in Cambodian live bird markets. This study conducted in limited sites documented the risk of reassortment between strains found in poultry and environment samples. Several serosurveys were implemented among 138 (at baseline) sellers and workers at live bird markets in Phnom Penh and Kampong Cham. These serosurveys identified seven cases of seroconversion for A(H5N1) in absence of severe clinical signs. These data will also contribute to an ongoing study on host vulnerability polymorphisms. The initial National Ethics Committee on Human Research approval #051 NECHR was granted on 20/02/2015.

Financial support Funded by DHHS

A(H5N1) case-control study A collaborative tabletop study is currently conducted on available data from Kantha Bopha hospitals to determine whether biological markers were associated with a positive A(H5N1) test result among pediatric SARI patients, adjusted for the time lapse between disease onset and testing.

strengthen preparedness, surveillance, threat detection and response, and thereby contribute to national and global health security

A poultry worker washes carcasses before butchering


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Financial support Self-funded

Rabies

Dogcount survey

Epi@ipc team leaders A. Tarantola, S. Ly

Objective and summary of results Cambodia is a highly rabies-endemic country where an estimated 810 human lives were lost to rabies in 2007 for an estimated incidence of 5.8/100,000 (95% CI 2.8-11.5). No routine canine vaccination has been implemented to date. Previous work conducted in the wake of a limited canine rabies vaccination campaign held in December 2012 – January 2014 in 15 Cambodian villages was unsatisfactory. Another study has been implemented in areas where the DVI dengue project is conducted. A total of 496 dogs from 395 households in a total of 15 villages of Kampot and Kompong Cham provinces will be monitored prospectively. This will enable us to document the dog population turnover and therefore the predicted herd immunity attrition rate if all had been immunized at baseline; informing the needed frequency of rabies dog vaccination campaigns.

Financial support Self-funded

Sick dogs exposure lookback and immune response study (RESIST)-I

Epi@ipc team leaders S. Ly, A. Tarantola

Objective and summary of study

Data is available showing that the immune response at D14 after a one-week protocol of three ID injections at D0, D3 and D7 is comparable to a 5-dose regimen. Clinical, epidemiological and biological data have been gathered to estimate whether the existing IM and ID protocol can be shortened (no D28 session) and reduced in doses (2 doses per session) in PrEP and in PEP protocols, at no risk to patients. After the 2013 lookback study in patients bitten by confirmed rabid dogs, this was extended in 2015 to patients bitten by sick-looking but rabies-untested dogs in 2003-2013. A total of 1153 persons bitten by sick-looking dogs in the rpc@ipc database, among which 1094 were included. Among these, 756 were alive

in good health, 10 were alive with ailments, 10 had died and the rest were lost to follow-up. These data will be analyzed in the first part of 2016.

Other

Ecology of Flying foxes and the risk of Nipah virus emergence in Cambodia

Epi@ipc team leaders J. Cappelle

Objective and summary of results This research project about the influence of flying foxes’ ecology on the risk of Nipah virus emergence in Cambodia provided several new results in 2015.

Results from urine sampling demonstrate that Nipah virus circulated within the colony in May for three years in a row, perhaps related to the reproduction cycle of the bats.

The study on the description and analysis of food behavior based on the analysis of bat fecal samples, in partnership with Prince of Songkla University (Thaïland), showed that mango and sapodilla fruits were an important part of flying foxes’ diet in that colony between April and June when Nipah virus was detected. These cultivated fruits may be a potential route of transmission from bats to humans.

The study on the analysis of the genetic structure of Pteropus populations in SEA, from ADN extraction in feces (in partnership with Chulalongkorn University , Thailand) did not show any geographical structuring based on mitochondrial DNA, suggesting that P. lylei in Thailand and Cambodia are part of the same population.

The preliminary anthropological study implemented in July 2014 in the village surrounding the bat roost showed that humans do not believe fruit bats can transmit diseases, but find it disgusting to eat a fruit partially eaten by a bat. Bats are hunted because their meat is tasty and their blood is thought to have therapeutic properties.

The longitudinal study with monthly captures and sampling of Pteropus lylei will continue in order to build our dataset and feed the mathematical model being developed. These data will allow to calibrate the model and to test the different mechanisms underlying the seasonal circulation of Nipah virus in bat populations.


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Main Partners:

Coordination by epi@ipc and CIRAD

In Cambodia: Forestry Administration, Royal University of Phnom Penh (RUPP), Fauna and Flora International (FFI)

In Thailand: Prince of Songkla University, Chulalongkorn University

Financial support

SEAe (below) and ComAcross projects

South East Asia encephalitis project (SEAe)

Japanese encephalitis dynamics in rural and periurban pigs in Cambodia.


Objective and summary of results

JE is mainly considered a rural disease, but there is growing evidence of peri-urban and urban transmission in several countries, including Cambodia. As a continuation of 2014 studies, two additional pig cohorts were followed-up from July to October 2015 in two locations: one in the same peri-urban site in Ta Khmau and one in a rural area in Kandal province in order to compare the circulation of JE in these two different landscapes. A French veterinary student (Master II) based in IPC from June to November 2015 and students from the Veterinary Faculty of the Royal University of Agriculture implemented these studies on the field.

Associated to this study on JE circulation in pigs, an entomological study was set up to compare the diversity and the population dynamics of the potential vectors of Japanese encephalitis around our peri-urban and rural pig farms. Monthly samplings started in July 2015 in both sites (peri-urban and rural) with 3 consecutive nights of capture at each site every month, using 5 lights traps on each site in and around the studied pig farms. This study was implemented by a master student at the Royal University of Phnom Penh and will last for one year until July 2016.

Main Partners:

CIRAD and epi@ipc

Financial support

SEAe project (Fondation Total, INSERM, AIRD, IRD, RIIP, and CIRAD).

Clinical epidemiology of encephalitis in three countries of the Mekong Region.

Epi@ipc team leaders Y. Crabol, J-D. Pommier, A. Tarantola

Objective and summary of results The South East Asia encephalitis study has concluded its pilot phase and is now fully implemented. It is coordinated from IPC and conducted at the Kantha Bopha hospital, Phnom Penh (Cambodia), as well as Mahosot Hospital, Vientiane (Lao PDR), and the National Hospital for Pediatrics, Hanoi (Vietnam). The initial National Ethics Committee on Human Research approval #0156 NECHR was granted on 26/08/2103.

This study brings together clinical and epidemiological data, lab data, state-of-the-art virology and wider perspectives from the social sciences and ecoepidemiology. Work Package 1 (Clinical and Field Epidemiology) is responsible for the rigorous identification and inclusion of infectious encephalitis cases, the collection and storage of clinical data and forwarding of biological samples to the lab for microbiological diagnosis. To 31st December, 2015, the study has included 81 patients in Lao PDR, 57 patients in Vietnam and 137 patients in Cambodia.

Main Partners:

LOMWRU, Institut Pasteur Paris, National Institute of Epidemiology and Hygiene in Vietnam.

Financial support

Fondation Total, INSERM, AIRD, IRD, RIIP, CIRAD.

ECOMORE

Cambodia

Epi@ipc team leaders J. Ledien, A. Tarantola

Objective and summary of results The Road use, Outbreak Amplification and Disease Surveillance (Roads) study is the implementation of the ECOnomic Development, ECOsystem MOdifications and Emerging Infectious Diseases Risk Evaluation (ECOMORE) project in Cambodia.


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It is a 3-year, multi-study project conducted in close collaboration with CNM aiming to stimulate and federate a dynamic of national collaborations to strengthen the Cambodian surveillance capacity on emerging vector-borne diseases, using Chikungunya and Dengue as proxy. The initial National Ethics Committee on, Human Research approval #0144 NECHR was granted on 26/08/2013.

Eight cities at crossroads in Cambodia have been selected as study sites: Takeo, Phnom Penh, Kampong Cham, Kampot, Battambang, Kratie, Kampong Chhnang and Kampong Speu. To 31st Dec 2015, the joint CNM/ECOMORE project has audited and helped capacitate the sentinel hospital network, extending it by 50%. Surveillance methods have been standardized and inter-sectoral collaborations strengthened. Feedback to participating sites and health departments has been improved for all components, increasing the acceptability of the system. SOP were formalized and distributed. Sentinel data was collected for the 2014 and 2015 Dengue seasons. Entomological data was collected. In collaboration with CDC-MoH, human syndromic surveillance data was successfully collected from the private sector began throughout 2015. Cell phone data was obtained thanks to a collaboration with a major Cambodian mobile phone provider.

Modeling of explanatory variables (weather, population density, and DENV genomics) and mobile telephone data as proxy for population movement will shed a light on the

determinants of dengue transmission in Cambodia and may help develop an early warning system.

Financial support Agence Française de Développement.

rospective: flagship projects to be implemented in 2016

A(H5N1) clinical review


Rationale and objectives In 2013 a clinical review workshop on A(H5N1) influenza provided an opportunity to compile and carefully review clinical, biological and radiological data in confirmed A(H5N1) cases. These data were all drawn from hospital clinical records using a single, complex but thorough questionnaire based on the ISARIC/WHO initial clinical form, completed with some items from an earlier IPC questionnaire (to include details on pulmonary auscultation, Glasgow scores and poultry exposure). These data are now being updated, verified and entered to perform a thorough analysis of clinical characteristics of A(H5N1) cases in 2013 compared to earlier years, as part of the assessment of the 2013 spike in confirmed cases.

Financial support None.

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A synoptic representation of the Road use, Outbreak Amplification and Disease Surveillance (Roads) study (ECOMORE Cambodia).


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Rabies PEP immune response study (RESIST) II


Rationale and objectives

Data is available showing that the immune response at D14 after a one-week protocol of three ID injections at D0, D3 and D7 is comparable to a 5-dose regimen. The number of doses administered in the one-week, three session protocol, however, remains high at 12 ID-doses. Clinical, epidemiological and biological data will be gathered to estimate whether the existing IM and ID protocol can be shortened (three-session, one-week regimen; no D28 session) and reduced in doses (6 instead of 8 total doses) in PrEP and in PEP protocols, at no risk to patients.

This will entail a targeted serological study in patients bitten by confirmed rabid dogs, to study antibody responses in the same patients after 3 sessions compared to 4 sessions (no shortening of WHO-recommended protocols for study purposes). This study, initially planned in 2015, was delayed due to incoherences between the authorization obtained from the Cambodian National Ethics Committee and the Pasteur Paris ethical committee.

Financial support Division International

Vaccine Match to Pneumococcal Strains (ViMPS)

Epi@ipc team leaders M. Inghammar, A. Tarantola

Rationale and objectives Streptococcus pneumoniae is a bacteria that can causes both severe disease and milder infections as well as asymptomatic colonization. Despite available antibiotics it is estimated that around 800,000 children die every year due to invasive pneumococcal disease, especially in developing countries where access to quality health care is limited. Pneumococci can be divided into over 90 different serotypes depending on differences in their capsular polysaccharides. The different serotypes have different potential for causing severe disease and their distribution varies with age, geographical area and time point. The incidence of invasive pneumococcal disease by the serotypes included in the vaccine has declined in high-income countries since the pneumococcal conjugate vaccine was introduced in the

childhood immunization schedule. After vaccine introduction, however, an increase of non-vaccine serotypes has been observed in various countries. This year, 2015, the pneumococcal conjugate vaccine was introduced in the childhood vaccination schedule in Cambodia. There is to date very limited data on the serotype distribution and incidence of invasive pneumococcal disease in Cambodia.

This collaborative study will involve Fondation Mérieux, Phnom Penh, Namru-2, Sihanouk Hospital Centre of Hope and Dr. Paul Turner, Angkor Hospital for Children (Cambodia-Oxford Medical Research Unit). It aims at mapping the serotype distribution of S. pneumoniae in Cambodia before the introduction of the conjugate vaccine, to serve as base-line data for future evaluation of the vaccination program. We will analyze stored samples of S. Pneumoniae that have been collected before December 31, 2014.

The initial National Ethics Committee on Human Research approval #460 NECHR was granted on 28/12/2015.

Financial support Swedish Governmental Funds for Research (ALF); Royal Swedish Physiographic Society, Lund; Fondation Mérieux, Phnom Penh.

Pertussis Immunization programmes in Low Income Countries (PERILIC)

Epi@ipc team leaders L. Borand, A. Tarantola

Rationale and objectives Infection by Bordetella pertussis or Bordetella parapertussis occurs in epidemic cycles and can cause severe acute respiratory disease especially in infants. Most pertussis cases occur in developing countries. A whole cell vaccine (wP) has been available since 1950s. In the post-vaccine era, incidence of clinical disease has declined more than 90% in the industrialized world. Nevertheless, during 2014 WHO listed pertussis as a major cause of death in infants globally, coincident with a global resurgence in pertussis incidence. This resurgence may have several causes including low or declining immunization coverage, timeliness, waning of vaccine-induced immunity with time, and emergence of vaccine-escape genotypic variants.

The aim of the study is to document Bordetella pertussis infection in children in four developing countries. This will enable


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researchers to : 1/ Document the clinical characteristics and prevalence rates of pertussis cases in children under 1 years old; and 2/ Assess immunization status among household contacts of index cases by determining the attack rate of pertussis infection and carriage.

Financial support Fondation Total.

Tenofovir As PRevention Of Hepatitis b transmission for Mothers (TA PROHM - ANRS 12345)

Epi@ipc team leaders L. Borand, A. Tarantola

Rationale and objectives Despite effective primary prophylaxis, HBV remains a substantial health problem both internationally and in Cambodia where neonatal transmission, unfortunately, still occurs. WHO recommends immediate administration of Hepatitis B vaccine and immunoglobulin in newborns to HBsAg+ mothers. Reported failure rates range from 1–14%, despite serovaccination. Factors associated with failure include HBeAg positivity and high HBV DNA VLs in the mother. Antiviral agents can be utilized to further decrease the risk of vertical transmission, especially in areas where WHO-recommended serovaccination are financially or operationally inaccessible. Several antiviral agents are currently presently used for this purpose. This project aims to implement PMTCT by reducing the HBV viral load in mothers by antivirals, typically initiated starting week 24 of pregnancy.

Financial support ANRS

A sculpted face looks out, at Ta Prohm Temple

Animal studies on zoonoses


Objective and summary of results A new study granted by the French Centre National d’Etudes Spatiales (CNES) will allow monitoring 20 flying foxes that will be equipped with GPS and satellite transmitters in 2016. The data collected will be used to map accurately the spatial distribution of these bats and to link it with the human activities and the ecology of Nipah virus studied in the SEAe project. This will allow us to identify high risk areas and persons at the bat-human interface. These persons will then be asked to be tested for Nipah virus antibodies with the serological that has been developed at IPC.

Additionally to this spatial study, the analysis of conditions and determinants of bat/human interface will allow identifying people and domestic animals at risk of Nipah infection. A questionnaire regarding people exposure and agricultural practices will be filled and serum samples will be collected and tested for Nipah virus antibodies at IPC.

A PhD student focusing on JE modeling started in October 2015. He will develop a generic model of JE circulation in order to integrate the different information we collected on the field and will test hypotheses on JE maintenance mechanisms in different landscapes. In a second phase, he will be able to develop optimization methods regarding the control of JE and of vector-borne diseases in general.

Field studies will be implemented in order to measure some specific parameters:

1/ A study of pigs and domestic birds JE seroprevalence will be implemented in different land-scapes of Cambodia. This study aims at comparing the circulation of JE in different landscape of Cambodia, urban and periurban areas, rural landscapes dominated by paddy fields and other rural landscapes such as forestry landscape. JE circulation will be assessed by comparing seroprevalences in piglets and in domestic birds (chickens and ducks).

2/ Entomological studies will be implemented in two different sites (periurban and rural) in order to measure key characteristics of the vector components. An ongoing longitudinal study will last for a year in order to assess the


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diversity and the population dynamics of the main JE vec-tor species in Cambodia. Another study will be implemented in order to assess the trophic preferences of these mosquito species.

Main Partners:

Coordination by epi@ipc and CIRAD

Financial support SEAe and ComAcross project

Development of a Lao PDR-Cambodia One Health Surveillance and Laboratory Network (LACANET)


Rationale and objectives The LACANET One Health Surveillance and Laboratory Network project (‘LACANET’) is an EU-funded project which brings together partners in the human health, wildlife health and animal health sectors to create capacity to survey, diagnose and understand the drivers of disease at human-animal-environmental interfaces. In Cambodia the project partners are Institut Pasteur du Cambodge (IPC), Wildlife Conservation Society (WCS) and the National Veterinary Research Institute (NaVRI). The project aims to support a bi-national, cross-sectoral Lao PDR-Cambodia One Health Surveillance and Laboratory network. It also includes human serosurveys in the field for rodent-borne zoonoses. These were carefully planned in 2015 and will be conducted in the coming months.

Financial support European Union

PAthogens’ NIChe: a new approach for infectious diseases control (PANIC)


Rationale and objectives The PANIC study aims at identifying foci for interepidemic transmission and spread of Dengue (“niches”). The aim of the study will be to assess the contribution of schools to DENV circulation in rural Cambodia through modeling.

Financial support Agence Nationale de la Recherche

upport to National authorities Knowledge transfer and application of findings to real-life control and prevention efforts are at the heart of epic@ipc’s research efforts. A list of projects conducted in the Unit with

practical implications and knowledge transfer can be found in Annex. In 2015, epi@ipc continued to actively support the Cambodian human and animal health authorities, at national or local levels.

Representatives from the epi@ipc regularly participated to Technical Working Group (TWG) meetings with the Ministry of Health (MoH) and the Ministry of Agriculture, Fisheries and Forestry (MAFF) and international stakeholders, especially on zoonoses. These were opportunities to offer support, expertise and advice when requested, delivered within the scope of operational meetings. Other venues were lengthier workshops, aiming for example to assess IHR preparedness with WPRO on 3-12 June 2015 (Asia Pacific Strategic Plan for Emerging Disease - APSED).

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Schoolchildren returning home on the road to Preah Vihear , Dec. 2013 (photo AT)


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Epi@ipc and the vaccination teams mobilized alongside the Cambodian authorities on World Rabies Day (September 28, 2015). Data and guidelines were shared. Several interviews were given to the written press as well as radio and TV programs. Messages were broadcast (and financed) through the Facebook page, reaching thousands of people (see Rabies Prevention Center 2015 Activity report).

Significant efforts also took place to support the development and implementation of the Cambodian Rabies Elimination Plan, following a workshop on March 16-19, 2015. IPC rabies data and experience were shared with human and animal health authorities. Access was shared freely on a Google Drive for World Rabies Day preparation meetings, many times postponed, held finally on July 10.

Three Cambodian students from CDC-MoH were strongly supported to integrate and attend the Pasteur Regional Rabies course co-organized by IPC in Oct-Nov 2015. The CDC-MoH focal point for the development of first-line rabies vaccination sites and WHO partners were invited at IPC on 22/12/2015 to examine and discuss the logistical, operational and budgetary aspects of maintaining a rabies vaccination center. We also hope to obtain funding to perform participatory modelling and health economics analyses to identify bottlenecks for timely access to adequate canine and human rabies vaccination.

As part of the ECOMORE syndromic surveillance system, and at participating clinicians’ request, clinical information sheets were forwarded weekly on the signs, epidemiology and treatment of targeted syndromes and diseases. These contributed to improving patient clinical management.

The Institut Pasteur du Cambodge, the Communicable Disease Control Department (CDC-MoH) and the National Immunization Program of the Ministry of Health (NIP-MoH) organized a symposium on the control of Japanese Encephalitis in Cambodia. This meeting was held at the Phnom Penh Hotel on September 15, 2015. More than 70 attendees from national authorities, hospitals in charge of Japanese Encephalitis case management or surveillance/control and international experts were present. The main objective of this symposium was to collect evidence of the burden of JEV in Cambodia and to share knowledge about the epidemiology of the disease. The symposium was funded by the European Union (Europe Aid program, ComAcross project) which among other things aims to reinforce capacities and accompany Cambodia in the control of JEV.

Participants of the Sept 15, 2015, meeting on JEV control in Cambodia.


cientific communications Below are presented all scientific articles published in peer-reviewed journals between January 1st and December 31st, 2015 with one or several epi@ipc staff members as coauthors.

Peer-reviewed articles published 1. Andries AC, Duong V, Ly S, Cappelle J, Kim KS, Lorn Try P, Ros S, Ong S, Huy R, Horwood P, Flamand M, Sakuntabhai A,

Tarantola A, Buchy P. Value of Routine Dengue Diagnostic Tests in Urine and Saliva Specimens. PLoS Negl Trop Dis. 2015 Sep 25;9(9):e0004100.

2. Barennes H, Slesak G, Goyet S, Aaron P, Srour LM (2015) Enforcing the International Code of Marketing of Breast‐milk Substitutes for better promotion of exclusive breastfeeding. Can lessons be learned? Journal of Human Lactation 2015 DOI: 10.1177/0890334415607816

3. Barennes H, Sengkhamyong K, René JP, Phimmasane M. Beriberi (thiamine deficiency) and high infant mortality in northern Laos. PLoS Negl Trop Dis. 2015 Mar 17;9(3):e0003581.

4. Barennes H, Frichittavong A, Gripenberg M, Koffi P. Evidence of High Out of Pocket Spending for HIV Care Leading to Catastrophic Expenditure for Affected Patients in Lao People's Democratic Republic. PLoS One. 2015 Sep 1;10(9):e0136664.

5. Barennes H, Phimmasane M, Rajaonarivo C. Insect Consumption to Address Undernutrition, a National Survey on the Prevalence of Insect Consumption among Adults and Vendors in Laos. PLoS One. 2015 Aug 28;10(8):e0136458.

6. Aurelie Binot, Raphael Duboz, Panomsak Promburom, Waraphon Primpapai, Julien Cappelle, Claire Lajaunie, Flavie Goutard, Tanu Pinyopummintr, Muriel Figuié, François Roger. Improving cross‐sectoral collaboration at the Animal/Human/Environment interface across stakeholders in Southeast Asia. The ComAcross (EU) project. One Health Journal December 2015 1 :44‐48

7. Cappelle J, Caron A, Servan de Almeida R, Gil P, Pedrono M, Mundava J, Fofana B, Balança G, Dakouo M, Ould El Mamy AB, Albonik C, Maminiaina F, Cumming G, De Visscher MN, Albina E, Chevalier V and Gaidet N. Empirical analysis suggests continuous and homogeneous circulation of Newcastle Disease virus in a wide range of Wild Birds species in Africa. Epidemiology and Infection. 2015, 143(6):1292‐303

8. Julien Cappelle, Flavie Luce Goutard, Marisa Peyre, François Roger. Collaborations sino‐européennes en santé animale : le projet LinkTADs et autres activités menées par le Cirad. Bulletin Epidemiologique. (Online first)

9. Caron A, Cappelle J, Cumming GS, de Garine‐Wichatitsky M, Gaidet, N. Bridge hosts, a missing link for disease ecology in multi‐host systems. Veterinary Research 2015, 46:83

10. Dim, B; Kerleguer, A; Kim, P; Pean, P; Phuong, V; Heng, N; Peng, Y; Borand, L; Tarantola, A. Necrotic tuberculin skin (Mantoux) test reaction: a case report and an estimation of frequency. Chest. 2015 Jul 1; 148(1):e1‐4.

11. Veasna Duong ; Louis Lambrechts ; Richard E Paul ; Sowath Ly ; Rath Srey Lay ; Kanya C. Long ; Rekol Huy; Arnaud Tarantola; Thomas W. Scott; Anavaj Sakuntabhai; Philippe Buchy. Asymptomatic humans transmit dengue virus to mosquitoes. Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):14688‐93.

12. Flavie Luce Goutard; Aurélie Binot; Raphael Duboz; Harena Rasamoelina‐Andriamanivo; Miguel Pedrono; Davun Holl; Marie‐Isabelle Peyre; Julien Cappelle; Véronique Chevalier; Muriel Figuié; Sophie Molia; François Roger. How to Reach the Poor? Surveillance in low‐income countries, lessons from experiences in Cambodia and Madagascar. PVM June 2015 120 (1) 12‐26

13. Goyet S, Touch S, Ir P, Saman S, Fassier T, Frutos R, Tarantola A and Barennes B. Gaps between research and public health priorities in low income countries: evidence from a systematic literature review focused on Cambodia. Implement Sci. 2015 Mar 11; 10(1):32.

14. Goyet S, Rammaert B, McCarron M, Khieu V, Fournier I, Kitsutani P, Ly S, Mounts A, Letson WG, Buchy P, Vong S. Mortality in Cambodia: an 18‐month prospective community‐based surveillance of all‐age deaths using verbal autopsies. Asia Pac J Public Health. 2015 Mar;27(2):NP2458‐70

15. Haridas V, Pean P, Jasenosky L.D., Madec Y, Laureillard D, Sok T, Sath S, Borand L, Marcy O, Chan S, Tsitsikov E, Delfraissy J‐F, Blanc F‐X,Goldfeld TB‐IRIS and remodelling of the T cell compartment in highly immunosuppressed HIV+ patients with TB: the CAPRI T (ANRS‐12614) study. A.E. AIDS. 2015 Jan 28;29(3):263‐73.

16. Hem S, Tarantola A, Chheang R, Nop P, Kerléguer A. Premier cas de gnathostomose intra‐oculaire à Gnathostoma spinigerum, diagnostiqué au Cambodge. Bull Soc Pathol Exot. 2015; 108(5):312‐315.

17. Naughtin M, Rith S, Sentilhes AC, Vong S, Joffret ML, Cornillot E, Deubel V, Delpeyroux F, Frutos R, Buchy P. Genetic diversity of human rhinoviruses in Cambodia during a three‐year period reveals novel genetic types. Infect Genet Evol. 2015 Jul 29; 35:42‐49.

18. Netrabukkana P., J. Cappelle, C. Trevennec, F. Roger, F. Goutard, P. Buchy, I.D. Robertson, S. Fenwick. Epidemiological Analysis of Influenza A Infection in Cambodian Pigs and Recommendations for Surveillance Strategies. Transboundary and Emerging Diseases, 2015 62(5)e37‐e44

19. Nouhin J, Barennes H, Madec Y, Prak S, Hou SV, Kerleguer A, Kim S, Pean P, Rouet F. Low frequency of acute hepatitis E virus (HEV) infections but high past HEV exposure in subjects from Cambodia with mild liver enzyme elevations, unexplained fever or immunodeficiency due to HIV‐1 infection. J Clin Virol. 2015 Oct;71:22‐7.

20. Marisa Peyre, Nicolas Gaidet, Alexandre Caron, Julien Cappelle, Annelise Tran, François Roger. 2015 Influenza aviaire dans le monde : situation au 31 Janvier. Bulletin Epidemiologique 67 :10‐14

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21. Schantz C, Sim KL, Ly EM, Barennes H, Sudaroth S, Goyet S. Reasons for routine episiotomy: A mixed‐methods study in a large maternity hospital in Phnom Penh, Cambodia. Reprod Health Matters. 2015 May;23(45):68‐77.

22. Slesak G, Inthalath S, Wilder‐Smith A, Barennes H. Road traffic injuries in northern Laos: trends and risk factors of an underreported public health problem. Trop Med Int Health. 2015 Nov;20(11):1578‐1587.

23. Takekawa J.Y., Heath S.R., Iverson S., Gaidet N., Cappelle J., Dodman T., Hagemeijer W., Eldridge W.D., Petrie S.A., Yarris G., Manu S., Olsen G.H., Prosser D.J., Spragens K.A., Douglas D.C., Newman S. 2015. Movement ecology of five Afrotropical waterfowl species from Malawi, Mali and Nigeria. Ostrich, 86 (1‐2) : p. 155‐168.

24. Tarantola A, Ly S, In S, Ong S, Peng Y, Heng N, Buchy P. Rabies Vaccine and Rabies Immunoglobulin in Cambodia: Use and Obstacles to Use. J Travel Med. 2015 Sep;22(5):348‐52.

Accepted in 2015 for publication in 2016 1. Hubert Barennes, Kang Virak, François Rouet, Yves Buisson, Michel Strobel, Ung Vibol. Factors associated with the failure of first and

second‐ line antiretroviral 1 therapies therapy in Cambodian HIV‐1 infected children 2. Beatriz Galatas; Sowath Ly; Veasna Duong; Kunthy Nguon; Siam Chan; Kathy Baisley ; Rekol Huy; Sovann Ly; Sopheak Sorn; Leakhann

Som; Philippe Buchy; Arnaud Tarantola. Long‐Lasting Immune Protection and Other Epidemiological Findings after Chikungunya Emergence in a Cambodian Rural Community, April 2012. Plos Neg Trop Dis. Accepted.

3. Paul F. Horwood, Alessio Andronico, Arnaud Tarantola, Henrik Salje, Veasna Duong, Channa Mey, Sovann Ly, Philippe Dussart, Simon Cauchemez, Philippe Buchy. Seroepidemiology of human enterovirus 71 infection among Cambodian children. Accepted Emerg Infect Dis. 2016 Jan;22(1):92‐5.

4. Olivier Marcy, Vibol Ung, Sophie Goyet, Laurence Borand, Philippe Msellati, Mathurin Tejiokem, Nguyen Thi Ngoc Lan, Boubacar Nacro, Sokleaph Cheng, Sara Eyangoh, Pham Thu Hang, Abdoul‐Salam Ouedraogo, Arnaud Tarantola, Sylvain Godreuil, Stéphane Blanche, Christophe Delacourt, for the PAANTHER study group. Performance of alternative specimen collection methods for the diagnosis of tuberculosis in HIV‐infected children using Xpert MTB/RIF. Accepted Clin Infect Dis.

5. Channa Mey, Artem Metlin, Veasna Duong, Sivuth Ong, Sotheary In, Paul F. Horwood, Jean‐Marc Reynes, Hervé Bourhy, Arnaud Tarantola, Philippe Buchy. Evidence of two distinct phylogenetic lineages of dog rabies virus circulating in Cambodia. Infection Genetics and Evolution 03/2016; 38. DOI:10.1016/j.meegid.2015.12.011

6. Arnaud Tarantola; Channa Mey; Voeungchan Sek; Sowath Ly; Cornelia Haener; Nareth Chhor; Uong Meng; Sovann Ly; Didier Fontenille, and Philippe Dussart. A confirmed rabies case in a French resident in Cambodia, June 2015. Accepted J Travel Med.

7. Arnaud Tarantola; Yoann Crabol ; Bangalore Jayakrishnappa Mahendra ; Sotheary In ; Hubert Barennes; Hervé Bourhy; Yiksing Peng; Sowath Ly; Philippe Buchy. Caring for rabies patients in developing countries ‐ the neglected importance of palliative care. Trop Med & Int Health. Accepted.

Abstracts presented at conferences and meetings 1. Julien Cappelle, Hul Vibol, Sébastien Ravon and Neil M. Furey. Flying foxes (Pteropus spp.) in Cambodia: colony

assessments and population dynamics. Annual Meeting of the ATBC Asia‐Pacific Chapter, Phnom Penh, Cambodia, 30 March ‐ 2 April 2015.

2. Hoem Thavry, Julien Cappelle, Lim Thona & Neil M. Furey. Diet and reproductive phenology of cave nectar bat (Eonycteris spelaea) in Cambodia and its conservation implications. Annual Meeting of the ATBC Asia‐Pacific Chapter, Phnom Penh, Cambodia, 30 March ‐ 2 April 2015.

3. Hoem Thavry, Julien Cappelle, Lim Thona, Hul Vibol & Neil M. Furey. Diet of the cave nectar bat Eonycteris spelaea in Cambodia. 3rd International Southeast Asian Bat Conference. Surawak, Malaysia, 14‐16 August 2015.

4. Hul Vibol, Hok Visal, Neil M. Furey & Julien Cappelle. Population dynamics and diet of Lyle’s flying fox (Pteropus lylei) in Cambodia. 3rd Int. Southeast Asian Bat Conference. Sarawak, Malaysia, 14‐16 August 2015.

5. Hul Vibol, Hok Visal, Neil M. Furey & Julien Cappelle. Diet of Lyle’s flying fox (Pteropus lylei) and potential transmission routes of Nipah virus in Cambodia. Annual Meeting of the ATBC Asia‐Pacific Chapter, Phnom Penh, Cambodia, 30 March ‐ 2 April 2015.

6. Lim Thona, Julien Cappelle, Hoem Thavry & Neil M. Furey. Cave selection and reproductive phenology of insectivorous bats in southern Cambodian karst and its conservation implications. Annual Meeting of the ATBC Asia‐Pacific Chapter, Phnom Penh, Cambodia, 30 March ‐ 2 April 2015.

7. Thongchai Ngamprasertwong, Vibol Hul, Julien Cappelle and Somsak Panha. Mitochondrial DNA structure of Lyle’s flying fox Pteropus lylei. 3rd International Southeast Asian Bat Conference. Sarawak, Malaysia, 14‐16 August 2015.

8. Louis Lambrechts, Veasna Duong, Richard Paul, Sowath Ly, Srey Rath Lay, Kanya C. Long, Chantha Ngan, Arnaud Tarantola, Thomas W. Scott, Anavaj Sakuntabhai, Philippe Buchy. Asymptomatic humans transmit dengue virus to mosquitoes. 64th annual meeting of the American Society for Tropical Medicine and Hygiene, Philadelphia, Pennsylvania, October 25‐29, 2015.

9. Richard Paul, Veasna Duong, Louis Lambrechts, Sowath Ly, Srey Rath Lay, Kanya C. Long, Chantha Ngang, Arnaud Tarantola, Thomas W. Scott, Anavaj Sakuntabhai, Philippe Buchy. Dengue transmission dynamics: the role of asymptomatic infections. Abstract 9th European Congress on Tropical Medicine and International Health (ECTMIH), 6‐10 September 2015, Basel, Switzerland.

10. M. Chan, P. Horwood, S. Ly, S. Rith, S. Sorn, K. Oeung, K. Nguon, S. Chan, P. Y, S. Ly, P. Dussart, P. Buchy, A. Tarantola. Seroprevalence studies to document transmission around identified human A(H5N1) cases, Cambodia, 2006‐2014. International Scientific Symposium ‐ Institut Pasteur International Network ‐ Paris, October 14‐16, 2015. Accepted, poster presentation.


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11. S Hem; S Ly; I Votsi, F Vogt; N Asgari; P Buchy; S Heng, M Picardeau; T Sok; S Ly, B Guillard; S Cauchemez; A Tarantola. Estimating the burden of leptospirosis among febrile subjects aged below 20 years in Kampong Cham communities, Cambodia, 2007‐2009. International Scientific Symposium ‐ Institut Pasteur International Network ‐ Paris, October 14‐16, 2015. Accepted, oral presentation.

12. C. Gorman, M. Lecuit, P. Newton, P. Dussart, P. Buchy, X. De Lamballerie, A. Tarantola, O. Lortholary, V. Chevalier, J. Cappelle, Y. Crabol. Southeast Asia Encephalitis Project.International Scientific Symposium ‐ Institut Pasteur International Network ‐ Paris, October 14‐16, 2015. Accepted, poster presentation.

13. Artem Metlin, Mey Channa, Duong Veasna, Sivuth Ong, Sotheary In, Paul F. Horwood, Philippe Dussart, Jean‐Marc Reynes, Hervé Bourhy, Sowath Ly, Arnaud Tarantola, Philippe Buchy. Phylogenetic Analysis of Dog Rabies Virus in Cambodia and in the Southeast Asian region. International Scientific Symposium ‐ Institut Pasteur International Network ‐ Paris, October 14‐16, 2015. Accepted, poster presentation.

14. SV Horm, S Rith, S Sorn, D Holl, S Ly, V Duong, L Allal, A Tarantola, PF Horwood, P Buchy. Human, Environmental and Animal Surveillance of Influenza A(H5N1) Virus in Cambodian Live Poultry Markets. 1st International Meeting on respiratory pathogens (IMRP), Singapore 2‐4 September 2015.

15. Yoann Crabol, Xavier De Lamballerie And Marc Lecuit, on behalf of the SEAe Consortium (Olivier Lortholary, Arnaud Tarantola, Xavier De Lamballerie, Philippe Dussart, Veronique Chevalier, Julien Capelle, Yoann Crabol, Christopher Gordman, Paul Newton, Marc Lecuit). "Encephalitis in the Mekong Region". Gordon Research Conference: Infections of the Nervous System ‐ Pathogenesis and Worldwide Impact. June 14‐19, 2015. The Chinese University of Hong Kong, Hong Kong, China. Poster.

16. Clara Champagne, David Salthouse, Sowath Ly, Arnaud Tarantola, Bernard Cazelles. Dengue dynamics in rural Cambodia: Comparing hypothesis through statistical and epidemiologic mechanistic model selection. Fifth International Conference on Infectious Disease Dynamics, December 1‐4, 2015, Clearwater Beach, FL, USA.

17. A. Tarantola. Old surveillance issues and new technologies: Using SMS to improve pharmacovigilance in the developing setting. NIH workshop on Developing Novel Strategies to Optimize Design of TB Drug Combinations – Washington DC , June 16‐17.

18. Paul F. Horwood, Alessio Andronico, Arnaud Tarantola, Henrik Salje, Veasna Duong, Channa Mey, Sovann Ly, Philippe Dussart, Simon Cauchemez, Philippe Buchy. Seroepidemiology of human enterovirus 71 infection among Cambodian children ‐ Investigating emergence. National Epidemiology Conference, Phnom Penh 16‐17 November,2015

19. Srey Viseth Horm, S Rith, S Sorn, D Holl, S Ly, V Duong, L Allal, A Tarantola, P Dussart, PF Horwood, P Buchy. Human, Environmental and Animal Surveillance of Influenza A(H5N1) Virus in Cambodian Live Poultry Markets. Oral presentation. National Epidemiology Conference, Phnom Penh 16‐17 November, 2015

20. S. Goyet; L. Borand; T. Chon; V. Ngo; A.Tarantola. Enjeux Ethiques dans le cadre d’un Programme de Recherche chez les Femmes Enceintes et les Nourrissons. International Symposium on “Ethics in Research for international Development: Environment, Social Issues and Health in the Countries of Greater Mekong Region”, University of Health Sciences, Vientiane (Laos), October 26‐27, 2015.

21. Dim Bunnet, Sophie Goyet, Laurence Borand, Nguyen Thi Ngoc Lan, Pham Thu Hang, Sylvain Godreuil, Ung Vibol, Olivier Marcy. High frequency of pulmonary non‐tuberculous mycobacteria in HIV‐infected children with a suspicion of tuberculosis in South‐East Asia. 46th Union World Conference on Lung Health 2015, Cape Town, South Africa.

22. Dim Bunnet, Sophie Goyet, Laurence Borand, Nguyen Thi Ngoc Lan, Pham Thu Hang, Sylvain Godreuil, Ung Vibol, Olivier Marcy. High frequency of pulmonary non‐tuberculous mycobacteria in HIV‐infected children with a suspicion of tuberculosis in South‐East Asia. 6th Vietnam Conference on HIV/AIDS 24‐26 Nov 2015. Poster.

23. Hubert Barennes, Kang Virak, François Rouet, Yves Buisson, Michel Strobel, Ung Vibol. Factors associated with the failure of first and second‐ line antiretroviral 1 therapies therapy in Cambodian HIV‐1 infected children. 9th European conference on Tropical Medicine and International Health. 6‐10 September 2015, Basel, Switzerland.

24. Champagne, C., Salthouse, D.G., Ly, S., Duong, V., Buchy, P., Tarantola, A. & Cazelles, B. 2015. Dengue dynamics in rural Cambodia: Comparing hypothesis through epidemiologic mechanistic model selection. Epidemics 5, Fifth International Conference on Infectious Disease Dynamics. Abstract Book O8.4, Clearwater Beach, Florida, December 2015.

Other 1. A. Tarantola, P. Dussart, Sovann Ly. RABIES ‐ CAMBODIA: STATUS REPORT, HUMAN, ANIMAL CASES. Promed Archive

Number: 20150630.3474734. Date: Fri 26 Jun 2015. Published Date: 2015‐06‐30 15:49:21 2. Final report: ‐‐ Seroprevalence Survey for Hand, Foot and Mouth Disease in Cambodia. Dr Paul Horwood, Dr Arnaud

Tarantola. Phnom Penh, November 2015. 3. Fortas C. Description and quantification of risk factors for symptomatic and asymptomatic cases of dengue infection

during community investigations in Kampong Cham Province, Cambodia, 2012 – 2013. Master thesis. EHESP. 4. Di Francesco‐ Isart J. Comparaison de la dynamique épidémiologique de l’encéphalite japonaise entre zones rurale et

péri ‐urbaine au Cambodge. Thèse professionnelle mastère spécialisé santé publique – spécialisation risque infectieux, CNAM EHESP, 9 décembre 2015

5. De Lauzanne A. Facteurs associés à l’infection pulmonaire a mycobactérie non tuberculeuse chez l’enfant de 0 à 13 ans infecté par le VIH suspect de tuberculose, dans 4 pays en développement de forte incidence tuberculeuse : Etude transversale secondaire à l’identification des souches de mycobactéries dans l’étude diagnostique PAANTHER 01 ‐‐‐ANRS 12229. Thèse mastère spécialisé santé publique – spécialisation risque infectieux, CNAM EHESP, 9 décembre 2015


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Annex: Impact Factor for 26/29 published or accepted epi@ipc articles (by first author) published or accepted in 2015*

* Three additional publications with no impact factor

Annex: ERA 2010 scores for 20/29 rated and published or accepted epi@ipc articles published or accepted, 2015 *3

* Nine other journals with no 2010 ERA rating

3 http://www.arc.gov.au/era/era_2012/archive/era_journal_list.htm ; http://lamp.infosys.deakin.edu.au/era/?page=jmain

0

2

4

6

8

10

12Duong

Marcy

Dim

Horw

ood

Haridas

Andries

Baren

nes

Galatas

Goyet

Caron

Baren

nes

Baren

nes

Nouhin

Mey

Naughtin

Netrabukkana

Goutard

Cappelle

Slesak

Tarantola

Schantz

Goyet

Tarantola

Tarantola

Takekawa

Hem

15%

20%

25%

40%

A* (top 5%): "Virtually all papers theypublish will be of a very high quality"

A (next 15%): "The majority of papersin a Tier A journal will be of very highquality"B (next 30%): "Generally, in a Tier Bjournal, one would expect only a fewpapers of very high quality"C (next 50%): Journals "that do notmeet the criteria of higher tiers"


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Annex: Available altmetrics for 8 articles in Plos or BMC journals (to 10 Jan. 2015)

Article ERA rating

Impact Factor

HTML page views

PDF downloads

XML downloads

Totals

Andries AC et al. Value of Routine Dengue Diagnostic Tests in Urine and Saliva Specimens. PLoS Negl Trop Dis. 2015 Sep 25;9(9):e0004100.

B 4.489 1608 279 6 1893

Barennes H et al. Beriberi (thiamine deficiency) and high infant mortality in northern Laos. PLoS Negl Trop Dis. 2015 Mar 17;9(3):e0003581.

B 4.489 1541 190 7 1738

Barennes H et al. Evidence of High Out of Pocket Spending for HIV Care Leading to Catastrophic Expenditure for Affected Patients in Lao People's Democratic Republic. PLoS One. 2015 Sep 1;10(9):e0136664.

A 3.234 580 75 9 664

Barennes H et al. Insect Consumption to Address Undernutrition, a National Survey on the Prevalence of Insect Consumption among Adults and Vendors in Laos. PLoS One. 2015 Aug 28;10(8):e0136458.

A 3.234 504 487 6 997

Goyet S et al. Gaps between research and public health priorities in low income countries: evidence from a systematic literature review focused on Cambodia. Implement Sci. 2015 Mar 11; 10(1):32.

C 4.122 2197

Duong V et al. Asymptomatic humans transmit dengue virus to mosquitoes. Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):14688‐93.

A* 9.7 1910

Total 4233 5138 28 5292


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Annex: Ongoing joint projects between epi@ipc and other IPC units, Dec. 2015.

Theme IB line Description Epi & PH Virology LABM G4 Immuno Epimol HIV/Hep Alim

H5N1 IB 05-41 Genetics and outbreak studies

DENFREE IB 05-44 Asymptomatic dengue

Dog count IB 05-45 Herd immunity attrition

MTB Camelia IB 05-47 Contagiosité et virulence souches

SEAe IB 05-53 Encephalitis

ECOMORE Cambodia. IB 05-54 Chik & Dengue along

roads

Birdy IB 05-60 AMR in newborns

Birdy - Klebsiella IB 05-67

Assess Klebsiella and AMR in mothers'

stools

ECOMORE Myanmar IB 08-46 SARI in pediatric

ICUs + HCW

STATIS IB 05-61 Empiric ttt of TB

Hept B PMTCT Pending Prospective study of teno PMTCT

ComAcross - Nipah IB 05-59 Bat ecology and

Nipah hotspots

LACANET Viro Detecting emergence in wlidlife

ComAcross IB 05-59 JEV, slaughterhouses and farm

ComAcross IB 05-59 Participatory modeling

Ev71 IB 08-03 Ev71 case control in community

Pneumococcus IB 05-76 ViMPS - Typing of S. pneumoniae strains.

RESIST IB 05-68-2

Immune factors and response to rabies

vax

Lookback sick dogs

IB 05-68-1

Outcome in sick dogs' bite victims, by N

sessions

PERILIC IB 05-77 Bordetella pertussis

DHHS3 IB 05-69 Market study

PANIC None Dengue modeling to identify hotspots

DVI / IVI IB 05-72 Health and econ burden of Dengue


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Annex: Transferring IPC research findings into policy A few 2014‐2015 examples among others

Institut Pasteur du Cambodge is a research institute of the Ministry of Health. As such it most often conducts research with a view to inform and guide responses to human health issues, including zoonoses. Researchers are not policymakers. Knowledge translation, however, is a priority for research units and teams which strive to usefully inform policymakers, program managers and clinicians who need to know in a timely way. This led even to a PhD on knowledge transfer in the epidemiology Unit4. In all cases colleagues from the relevant Ministries and/or other national stakeholders are closely associated with projects and publications.

Study IPC unit(s)

Topic Liaising with national stakeholders

Camelia (completed)

Epi + viro

Early vs. late ARV National HIV management guidelines changed

ECOMORE (ongoing)

Epi + viro

Early warning for dengue

CNM and CDC-Cam both co-PI; disseminated SOP; enlarged & strengthened sentinel system; syndromic surveillance in communities; support data collection for national dengue weekly bulletin

Rabies (ongoing)

Epi + viro

Various Part of National Rabies Elimination Plan (secondary sites) ; education of public and practitioners ; Only rabies surveillance data in Cambodia, handed each year to authorities

PAANTHER (ongoing)

Epi + Viro + LABM

Strategies to diagnose TB

NPH is co-PI ; implementation on novel sampling techniques at National Pediatric Hospital

CALIBAN (completed)

Epi + LABM

Assessment of AMR in community-acquired pneumonia

MoH part of working group; National recommendations for Pediatric CAP management changed

DHHS (ongoing)

Epi + Viro

A(H5N1) outbreaks, surveillance, serostudies

Monitoring transmission, reassortment and confirming positive samples (A/H5N1 WHO collaborating center); Reports to MoH; participation to national zoonosis working group;

SISEA (completed)

Epi + Viro + LABM

Surveillance of community-acquired pneumonia

National surveillance bulletin handed to MoH (done by WHO) ; first workshop on melioidosis for clinicians; training of Cambodian Master students on systematic reviews

SEAe / ComAcross (ongoing)

Epi + Viro

Nipah and bat studies

Conducted with Dept Wildlife of Min of Agriculture, Forestry & Fisheries.

4 Sophie Goyet. Modélisation du processus d'application des connaissances entre recherche et santé publique. Santé publique et épidémiologie. Université Montpellier II, 2014. French.<tel-01131672> Available at https://tel.archives-ouvertes.fr/tel-01131672/document


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Ev71 (ongoing)

Epi + Viro

Identifying Ev71 emergence and risk factors

Mandated by MoH and funded by ADB

DVI (ongoing)

Epi + Viro

Dengue burden studies

Conducted with national Dengue program at CNM (co-investigator); support of sentinel sites

SEAe (ongoing)

Epi + Viro

Encephalitis in children

Co-PI from MoH ; much improved first-line testing for treatable etiologies in participating hospital

Chikungunya (completed)

Epi + Viro

Outbreak investigation & spinoffs

Expertise prior to outbreak investigations; Outbreak investigation report and recommendations to CNM; modeling to derive R0 and evidence long-lasting immune protection, associating CNM

DENFREE (ongoing)

Epi + Viro (ongoing)

Several WPs CNM co-investigator in Cambodia ; support to CNM sentinel surveillance

STATIS (ongoing)

Epi + LABM

Targeted vs. systematic treatment of TB in newly discovered HIV cases

NCHADS medical doctor is co-PI; active finding of yet untreated HIV cases

ECOMORE Myanmar

Viro + Epi

SARI in pediatric wards

SARI chosen as study theme at the request of the Minister of Health of the Union of Myanmar.

Lepto study (completed)

Epi + LABM

Prevalence of leptospirosis in Kg Cham

Serostudy on biobanked samples; PharmD thesis at University; Co-authors from CDC-MoH and from CNM

Dengue (ongoing)

Epi + Viro

Testing dengue-suspect patients

Monitoring epidemiological trends for CNM to detect outbreaks and changes in circulating serotypes

Not involving epi@ipc :

Study IPC unit(s)

Topic Liaising with national stakeholders

LABM (ongoing)

LABM Microbiology and antimicrobial resistance

Participation to permanent MoH AMR committee; as well as SOP expertise for WHO/MoH lab network

HIV/Hep unit HIV/Hep unit

Roka outbreak Documenting the extent of the Roka HIV nosocomial outbreak in close cooperation with authorities. Epi participated to initial investigation.

LABM (ongoing)

LABM Microbiology and antimicrobial resistance

Participation to permanent MoH AMR committee; as well as SOP expertise for WHO/MoH lab network

Malaria Malaria Unit

Artemisinin resistance et al.

Monitoring artemisinin resistance with CNM and quality testing of RDTs ; participation in malaria serosurveys with CNM

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Documents

Epidemiology & Public Health Unit (epi@ipc) 2015 · Unit (epi@ipc) 2015 The scientific & activity report of the Epidemiology & Public Health Unit of the Institut Pasteur du Cambodge