Abstract — WCN 2013No: 479Topic: 36 — Other topicsRabies virus infection is associated withmitochondrial dysfunction

A.C. Jacksona, T. Alandijanyb, W. Kammounic, S. Roy Chowdhuryd,P. Fernyhoughe. aInternal Medicine (Neurology) andMedical Microbiology,University of Manitoba, Canada; bMedical Microbiology, University ofManitoba, Canada; cInternal Medicine, University of Manitoba, Canada;dNeurodegenerative Disorders, St. Boniface Hospital Research Centre,Canada; ePharmacology and Therapeutics, University of Manitoba,Winnipeg, MB, Canada

Studies in an experimentalmodel of rabies have shownmajor structuralchanges in the brain involving neuronal processes that are associatedwith severe clinical disease. Cultured adult mouse dorsal root ganglion(DRG) neurons are permissive to infection with the CVS strain of rabiesvirus (RABV). RABV-infected DRG neurons show axonal swellings and4-hydroxy-2-nonenal protein adduct immunostaining, indicating evi-dence of oxidative stress, and also reduced axonal growth versusmock-infected DRG neurons. We hypothesized that RABV infection inducesmitochondrial dysfunction leading to oxidative stress. We investigatedthe effects of RABV infection on mitochondrial parameters. In RABVinfection there were increased activities of electron transport chainComplexes I and IV versus mock infection, whereas Complexes II–III,citrate synthase, and malate dehydrogenase activities were unchanged.Increases in Complex I activity, but not Complex IV activity, correlatedwith cellular susceptibility to RABV infection. Mitochondrial respi-ration studies showed that RABV infection increased maximaluncoupled respiration and Complex IV respiration, whereas coupledrespiration and the rate of proton leak were unchanged. A highmitochondrial membrane potential was generated. RABV infectionreduced the intracellular ATP level and altered the cellular redoxstate with a high NADH/NAD+ ratio. In RABV-infected neurons,basal production of reactive oxygen species (ROS) was unaffected,but a higher rate of ROS generation occurred with mitochondrialsubstrates and inhibitors. We conclude that RABV infection inducesmitochondrial dysfunction leading to ROS overgeneration and oxidativestress. We postulate that the fundamental abnormality is due to theinteraction of the RABV phosphoprotein with Complex I leading to itsincreased activity.

doi:10.1016/j.jns.2013.07.2123

Abstract — WCN 2013No: 1029Topic: 36 — Other topicsEpidemiology of tuberculosis of central nervous system inpatients with HIV-infection in the Irkutsk Region of Russia

Y.N. Bykova, T.P. Filippovab, O.N. Novitskayac. aNeurology, Irkutsk StateMedical University, Russia; bPhthisiology, Irkutsk State Medical University,Russia; cPhthisiology, Irkutsk Regional Tuberculosis Hospital,Irkutsk, Russia

Background: Increasing prevalence of tuberculosis infection in theIrkutsk Region of Russia remains one of the most common problems.Objective: This study presents results of epidemiological investiga-tion devoted to the comorbidity of tuberculosis and HIV-infection inthe Irkutsk Region.Patients and methods: 240 patients (184 males and 56 females)aged from 20 to 56 have been observed.

1. Group of patients with tuberculosis of central nervous system andHIV-infection (196 patients).

2. Group of patients with tuberculosis of central nervous systemwithout HIV-infection (44 patients).

Results:1st group:Clinical forms of tuberculosis of the central nervous system: meningitis— 124 (63.3 ± 3.4%); meningo-encephalitis — 66 (33.7 ±3.4%);meningo-myelitis — 1 (0.5% ± 0.5%); meningo-encephalo-myelitis— 4 (2.0 ±1.0%); tuberculoma of the brain — 1 (0.5% ± 0.5%).HIV-associated diseases (n= 181): candidosis of the gastro-intestinalsystem — 178 (90.8%), pneumocystic pneumonia— 3 (1.5%).Social characteristics: drug abused (injecting drug users) — 158(80.6 ± 2.8%), alcohol abused — 14 (7.1 ± 1.8%).

2nd group:Clinical forms of tuberculosis of central nervous system: meningitis— 38 (86.4 ± 5.0%); meningo-encephalitis— 2 (4.6 ± 3.0%); meningo-myelitis — 3 (6.8% ± 4.0%); meningo-encephalo-myelitis — 1 (2.3 ±2.0%); tuberculoma of the brain — 0.Social characteristics: drug abused (injecting drug users) — 3 (6.8 ±4.0%), alcohol abused — 7 (16.0 ± 3.0%).Conclusion: Obtained data show severe epidemiological situation oftuberculosis in patients with HIV-infection in the Irkutsk Region ofRussia. Frequent combination of central nervous system tuberculosisand HIV-infection was revealed. There was comorbidity withmeningo-encephalitis with generalized tuberculosis infection com-bined with candidosis, and hepatitis in drug abused patients withHIV-infection.

doi:10.1016/j.jns.2013.07.2124

Abstract — WCN 2013No: 1023Topic: 36 — Other topicsThe value of preoperative fMRI in drawing attention to possibleunexpected language area representations in a patientwith anaplastic astrocytoma

M. Jehnaa, C. Enzingera,b, G. von Campec, M. Hoffermannc,L. Bruckmannc, G. Reishoferd, F. Payerb, F. Ebnera. aDivision ofNeuroradiology, Department of Radiology, Medical University of Graz,Graz, Austria; bDepartment of Neurology, Medical University of Graz, Graz,Austria; cDepartment of Neurosurgery, Medical University of Graz, Graz,Austria; dDepartment of Radiology, Medical University of Graz, Graz, Austria

This is a case study of a right handed patient with a grade III anaplasticastrocytoma in the superior frontal cortexwith intra-axial extensions tothe corpus callosum. Presurgical functional MR imaging (fMRI) wasdone to visualize language network distribution. Intraoperative directcortical stimulation (DCS) was accordingly done to prevent eloquentfunctional regions from resection. fMRI results showed a distributedlanguage network including the left inferior frontal gyrus (parsopercularis), the left and right frontal orbital cortices, the left insularcortex, the left superior and middle temporal gyri, the left precentraland postcentral gyri, and the supplementary area. Additionally,statistically stable but unusual activation was found in the left superiorfrontal gyrus (SFG). During surgery, DCS demonstrated that thestimulation of the SFG – involved in language function according tothe fMRI – led to anomic disturbances. These deficits in word findingwere persistently reproducible when stimulating the same area.Afterwards, tumor removal was performed under navigation andfluorescence guidance as well as subcortical electrostimulation, thuspreserving eloquentmotor and language areas and tracts. Regarding theresults of the fMRI and DCS in this patient, it can be speculated that thealtered distribution of eloquent language areas could be the result of

Abstracts / Journal of the Neurological Sciences 333 (2013) e579–e628 e609