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EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but are out of order. 1. Place both trays in sunlight for one week. 2. Spray 100 mL of 100% fertilizer solution over tray A. Spray 100 mL of 50% fertilizer solution over tray B. 3. Measure the biomass of the grass. Record observations in a data table. 4. Fill two trays with soil and plant an equal number of grass seeds. In which order should the class perform these steps? A) 2 – 1 – 3 – 4 B) 2 – 4 – 3 – 1 C) 4 – 1 – 2 – 3

EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

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Page 1: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PRACTICEA class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but are out of order.

1. Place both trays in sunlight for one week.

2. Spray 100 mL of 100% fertilizer solution over tray A.

Spray 100 mL of 50% fertilizer solution over tray B.

3. Measure the biomass of the grass. Record observations in a data table.

4. Fill two trays with soil and plant an equal number of grass seeds.

In which order should the class perform these steps?

A) 2 – 1 – 3 – 4

B) 2 – 4 – 3 – 1

C) 4 – 1 – 2 – 3

D) 4 – 2 – 1 – 3

Page 2: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PRACTICEA class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but are out of order.

1. Place both trays in sunlight for one week.

2. Spray 100 mL of 100% fertilizer solution over tray A.

Spray 100 mL of 50% fertilizer solution over tray B.

3. Measure the biomass of the grass. Record observations in a data table.

4. Fill two trays with soil and plant an equal number of grass seeds.

In which order should the class perform these steps?

A) 2 – 1 – 3 – 4

B) 2 – 4 – 3 – 1

C) 4 – 1 – 2 – 3

D) 4 – 2 – 1 – 3

Page 3: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PRACTICEWhich graph correctly illustrates the growth of a population when resources are unlimited?

A)

D)C)

B)

Page 4: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PRACTICEWhich graph correctly illustrates the growth of a population when resources are unlimited?

A)

D)C)

B)

Page 5: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

What You’ll Learn

You will relate the structure of DNA to its function.

You will explain the role of DNA in protein production.

You will distinguish among different types of mutations.

Page 6: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• Analyze the structure of DNA

Section Objectives:

• Determine how the structure of DNA enables it to reproduce itself accurately.

Page 7: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• Although the environment influences how an organism develops, the genetic information that is held in the molecules of DNA ultimately determines an organism’s traits.

• DNA achieves its control by determining the structure of proteins.

What is DNA?What is DNA?

Page 8: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• All actions, such as eating, running, and even thinking, depend on proteins called enzymes.• Enzymes are critical for an organism’s function because they control the chemical reactions needed for life.

What is DNA?What is DNA?

• Within the structure of DNA is the information for life—the complete instructions for manufacturing all the proteins for an organism.

Page 9: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• In 1952 Alfred Hershey and Martha Chase performed an experiment using radioactively labeled viruses that infect bacteria.

• These viruses were made of only protein and DNA.

DNA as the genetic materialDNA as the genetic material

Page 10: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• Hershey and Chase labeled the virus DNA with a radioactive isotope and the virus protein with a different isotope.

DNA as the genetic materialDNA as the genetic material

• By following the infection of bacterial cells by the labeled viruses, they demonstrated that DNA, rather than protein, entered the cells and caused the bacteria to produce new viruses.

Page 11: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• DNA is a polymer made of repeating subunits called nucleotides.

• Nucleotides have three parts: a simple sugar, a phosphate group, and a nitrogenous base.

Phosphate group

Sugar (deoxyribose)

Nitrogenous base

The structure of nucleotidesThe structure of nucleotides

Page 12: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• The phosphate group is composed of one atom of phosphorus surrounded by four oxygen atoms.

• The simple sugar in DNA, called deoxyribose (dee ahk sih RI bos), gives DNA its name—deoxyribonucleic acid.

The structure of nucleotidesThe structure of nucleotides

Page 13: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• A nitrogenous base is a carbon ring structure that contains one or more atoms of nitrogen.

• In DNA, there are four possible nitrogenous bases: adenine (A), guanine (G), cytosine (C), and thymine (T).

Adenine (A) Guanine (G) Thymine (T)Cytosine (C)

The structure of nucleotidesThe structure of nucleotides

Page 14: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• Thus, in DNA there are four possible nucleotides, each containing one of these four bases.

The structure of nucleotidesThe structure of nucleotides

Page 15: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• Nucleotides join together to form long chains, with the phosphate group of one nucleotide bonding to the deoxyribose sugar of an adjacent nucleotide.

• The phosphate groups and deoxyribose molecules form the backbone of the chain, and the nitrogenous bases stick out like the teeth of a zipper.

The structure of nucleotidesThe structure of nucleotides

Page 16: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• In DNA, the amount of adenine is always equal to the amount of thymine, and the amount of guanine is always equal to the amount of cytosine.

The structure of nucleotidesThe structure of nucleotides

Page 17: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• In 1953, Watson and Crick proposed that DNA is made of two chains of nucleotides held together by nitrogenous bases.

The structure of DNAThe structure of DNA

• Watson and Crick also proposed that DNA is shaped like a long zipper that is twisted into a coil like a spring.

• Because DNA is composed of two strands twisted together, its shape is called double helix.

Page 18: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

The importance of nucleotide sequencesThe importance of nucleotide sequences

Chromosome

The sequence of nucleotides forms the unique genetic information of an organism. The closer the relationship is between two organisms, the more similar their DNA nucleotide sequences will be.

Page 19: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

The importance of nucleotide sequencesThe importance of nucleotide sequences

• Scientists use nucleotide sequences to determine evolutionary relationships among organisms, to determine whether two people are related, and to identify bodies of crime victims.

Page 20: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

Replication of DNAReplication of DNA

• Before a cell can divide by mitosis or meiosis, it must first make a copy of its chromosomes.

• The DNA in the chromosomes is copied in a process called DNA replication.

• Without DNA replication, new cells would have only half the DNA of their parents.

Page 21: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

Replication of DNA

Replication of DNA

DNA

Replication

Replication

Page 22: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

Replication of DNA

Replication of DNA

Page 23: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• DNA is copied during interphase prior to mitosis and meiosis.

• It is important that the new copies are exactly like the original molecules.

Copying DNACopying DNA

Page 24: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

Copying DNACopying DNA

Original DNA

Original DNA

Strand

Original DNA

Strand

Free Nucleotides New DNA

moleculeNew DNA

Strand

New DNA molecule

Page 25: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

Question 1

What importance did the experiment performed by Alfred Hershey and Martha Chase have in determining what genetic material was?

Many scientists believed protein was the genetic material. However, an experiment using radioactively labeled viruses allowed Hershey and Chase to provide convincing evidence that DNA is the genetic material.

Answer

Page 26: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

Question 2

Which of the following is NOT a component of DNA?

D. proteins

C. nitrogenous bases

B. phosphate groups

A. simple sugars

The answer is D.

Page 27: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

Question 3

Which of the following correctly comprises a complimentary base pair?

D. cytosine – thymine

C. guanine – adenine

B. thymine – guanine

A. adenine – thymine

The answer is A.

Page 28: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

A student studying four different organisms recorded this data.

Characteristics of Four Organisms

Organism Food Habitat Same Segment of DNA Time Most Active

1 Small Animals Rivers and lakes AAA CGG TAC AAA Day

2 Grasses and leaves Meadow ATT GCG TAA AAA Day and night

3 Grasses Forest AAA CGG TAA AAA Night

4 Insects and plants Forest ATA GGG TAC AAA Day

Which organisms are most closely related?

A) Organisms 3 and 4 B) Organisms 2 and 3

C) Organisms 1 and 4 D) Organisms 1 and 3

EOI PRACTICE

Organism Food Habitat Same Segment of DNA Time Most Active

1 Small Animals Rivers and lakes AAA CGG TAC AAA Day

2 Grasses and leaves Meadow ATT GCG TAA AAA Day and night

3 Grasses Forest AAA CGG TAA AAA Night

4 Insects and plants Forest ATA GGG TAC AAA Day

Page 29: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

A student studying four different organisms recorded this data.

Characteristics of Four Organisms

Organism Food Habitat Same Segment of DNA Time Most Active

1 Small Animals Rivers and lakes AAA CGG TAC AAA Day

2 Grasses and leaves Meadow ATT GCG TAA AAA Day and night

3 Grasses Forest AAA CGG TAA AAA Night

4 Insects and plants Forest ATA GGG TAC AAA Day

Which organisms are most closely related?

A) Organisms 3 and 4 B) Organisms 2 and 3

C) Organisms 1 and 4 D) Organisms 1 and 3

EOI PRACTICE

Page 30: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeA scientist wanted to determine the best level of light intensity for photosynthesis in an aquatic plant. He varied the light intensity and measured the amount of a gas produced by the plant at each level of light intensity.

Which is the dependent variable in this experiment?

A) the type of plant

B) the light intensity

C) the amount of oxygen produced

D) the amount of carbon dioxide produced

Page 31: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeA scientist wanted to determine the best level of light intensity for photosynthesis in an aquatic plant. He varied the light intensity and measured the amount of a gas produced by the plant at each level of light intensity.

Which is the dependent variable in this experiment?

A) the type of plant

B) the light intensity

C) the amount of oxygen produced

D) the amount of carbon dioxide produced

Page 32: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeA biologist is studying a deer herd living in a 100-square-kilometer wildlife area. He determines that the current population of 3000 deer is half the carrying capacity of the wildlife area.

What would the deer population density be if the population were at carrying capacity?

A) 15 deer per square kilometer

B) 60 deer per square kilometer

C) 1500 deer

D) 6000 deer

Page 33: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeA biologist is studying a deer herd living in a 100-square-kilometer wildlife area. He determines that the current population of 3000 deer is half the carrying capacity of the wildlife area.

What would the deer population density be if the population were at carrying capacity?

A) 15 deer per square kilometer

B) 60 deer per square kilometer

C) 1500 deer

D) 6000 deer

Page 34: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI Practice

What are alleles?

(a) different parts of one gene

(b) different forms of one gene

(c) different genes that affect the same trait

(d) different chromosomes with the same

genes

Page 35: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI Practice

What are alleles?

(a) different parts of one gene

(b) different forms of one gene

(c) different genes that affect the same trait

(d) different chromosomes with the same

genes

Page 36: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeA scientist compared the genes and proteins from a root cell and a leaf cell of a single apple tree. What will the scientist observe about the cells?

(a) They have different sets of genes but the same set of proteins.

(b) They have different sets of genes and different sets of proteins.

(c) They have the same set of genes and the same set of proteins.

(d) They have the same set of genes but different sets of proteins.

Page 37: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeA scientist compared the genes and proteins from a root cell and a leaf cell of a single apple tree. What will the scientist observe about the cells?

(a) They have different sets of genes but the same set of proteins.

(b) They have different sets of genes and different sets of proteins.

(c) They have the same set of genes and the same set of proteins.

(d) They have the same set of genes but different sets of proteins.

Page 38: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeHuman parents have 46 chromosomes. Why do human offspring have 46 chromosomes instead of 92 chromosomes?

(a) Only the male parent contributes chromosomes to the offspring.

(b) Only the female parent contributes chromosomes to the offspring.

(c) Both parents contribute only one-half of their chromosomes to the offspring.

(d) Only half of the chromosomes that the male and female parents contribute survive in the offspring.

Page 39: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeHuman parents have 46 chromosomes. Why do human offspring have 46 chromosomes instead of 92 chromosomes?

(a) Only the male parent contributes chromosomes to the offspring.

(b) Only the female parent contributes chromosomes to the offspring.

(c) Both parents contribute only one-half of their chromosomes to the offspring.

(d) Only half of the chromosomes that the male and female parents contribute survive in the offspring.

Page 40: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI Practice

DNA contains direct instructions for how to build which part of a cell?

(a) lipids

(b) nucleus

(c) proteins

(d) carbohydrates

Page 41: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI Practice

DNA contains direct instructions for how to build which part of a cell?

(a) lipids

(b) nucleus

(c) proteins

(d) carbohydrates

Page 42: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeBelow is a model of DNA.

Which part of DNA contains genetic information?

(a) the tightness of the coils

(b) the total number of base pairs

(c) the order of sugars and phosphates

(d) the order of different kinds of bases

Page 43: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeBelow is a model of DNA.

Which part of DNA contains genetic information?

(a) the tightness of the coils

(b) the total number of base pairs

(c) the order of sugars and phosphates

(d) the order of different kinds of bases

Page 44: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeThe crossing between two heterozygous tall pea plants (Tt) produces both tall and dwarf pea plants in a 3:1 ratio. Which process leads to the production of offspring in this phenotypic ratio?

(a) replication of alleles during mitosis

(b) incomplete dominance of the alleles

(c) segregation of alleles during meiosis

(d) mutation occurring within the alleles

Page 45: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeThe crossing between two heterozygous tall pea plants (Tt) produces both tall and dwarf pea plants in a 3:1 ratio. Which process leads to the production of offspring in this phenotypic ratio?

(a) replication of alleles during mitosis

(b) incomplete dominance of the alleles

(c) segregation of alleles during meiosis

(d) mutation occurring within the alleles

Page 46: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeA disorder exists in which the pairing of two recessive alleles, rr, is harmful. Which cross will lead to the greatest number of offspring that are unaffected carriers of this disorder?

(a) rr × rr

(b) rr × Rr

(c) RR × rr

(d) RR × RR

Page 47: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

EOI PracticeA disorder exists in which the pairing of two recessive alleles, rr, is harmful. Which cross will lead to the greatest number of offspring that are unaffected carriers of this disorder?

(a) rr × rr

(b) rr × Rr

(c) RR × rr

(d) RR × RR

Page 48: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

Section Objectives

• Sequence the steps involved in protein synthesis.

• Relate the concept of the gene to the sequence of nucleotides in DNA.

Page 49: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• The sequence of nucleotides in DNA contain information.

Genes and ProteinsGenes and Proteins

• This information is put to work through the production of proteins.

• Proteins fold into complex, three- dimensional shapes to become key cell structures and regulators of cell functions.

Page 50: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• Some proteins become important structures, such as the filaments in muscle tissue.

• Other proteins, such as enzymes, control chemical reactions that perform key life functions—breaking down glucose molecules in cellular respiration, digesting food, or making spindle fibers during mitosis.

Genes and ProteinsGenes and Proteins

Page 51: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• Thus, by encoding the instructions for making proteins, DNA controls cells.

• In fact, enzymes control all the chemical reactions of an organism.

Genes and ProteinsGenes and Proteins

Page 52: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• You learned earlier that proteins are polymers of amino acids.

• The sequence of nucleotides in each gene contains information for assembling the string of amino acids that make up a single

protein.

Genes and ProteinsGenes and Proteins

Page 53: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• RNA like DNA, is a nucleic acid. RNA structure differs from DNA structure in three ways.

• First, RNA is single stranded—it looks like one-half of a zipper —whereas DNA is double stranded.

RNARNA

Page 54: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• The sugar in RNA is ribose; DNA’s sugar is deoxyribose.

Ribose

RNARNA

Page 55: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• Both DNA and RNA contain four nitrogenous bases, but rather than thymine, RNA contains a similar base called uracil (U).

Uracil

Hydrogen bonds Adenine

RNARNA

Page 56: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• DNA provides workers with the instructions for making the proteins, and workers build the proteins.

• The workers for protein synthesis are RNA molecules.

• They take from DNA the instructions on how the protein should be assembled, then—amino acid by amino acid—they assemble the protein.

RNARNA

Page 57: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• There are three types of RNA that help build proteins.

• Messenger RNA (mRNA), brings instructions from DNA in the nucleus to the cell’s factory floor, the cytoplasm.

• On the factory floor, mRNA moves to the assembly line, a ribosome.

RNARNA

Page 58: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• The ribosome, made of ribosomal RNA (rRNA), binds to the mRNA and uses the instructions to assemble the amino acids in the correct order.

RNARNA

Page 59: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• Transfer RNA (tRNA) is the supplier. Transfer RNA delivers amino acids to the ribosome to be assembled into a protein.

RNARNA

Page 60: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

TranscriptionTranscription• In the nucleus, enzymes make an RNA copy

of a portion of a DNA strand in a process called transcription.

Page 61: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

RNA strand

DNA strand

DNA strand

RNA strand

TranscriptionTranscription

A

B

C

Page 62: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

TranscriptionTranscription

• The main difference between transcription and DNA replication is that transcription results in the formation of one single-stranded RNA molecule rather than a double-stranded DNA molecule.

• The mRNA leaves the nucleus and travels to the ribosome.

Page 63: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• The nucleotide sequence transcribed from DNA to a strand of messenger RNA acts as a genetic message, the complete information for the building of a protein.

• As you know, proteins contain chains of amino acids.

The Genetic CodeThe Genetic Code

Page 64: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• Biochemists began to crack the genetic code when they discovered that a group of three nitrogenous bases in mRNA code for one amino acid. Each group is known as a codon.

• A code is needed to convert the language of mRNA into the language of proteins.

The Genetic CodeThe Genetic Code

Page 65: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• Sixty-four combinations are possible when a sequence of three bases is used; thus, 64 different mRNA codons are in the genetic code.

The Genetic CodeThe Genetic Code

Page 66: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

The Messenger RNA Genetic CodeFirst Letter Second Letter

U

U C A G

Third Letter

U

C

A

G

U

C

A

G

U

C

A

G

U

C

A

G

C

A

G

Phenylalanine (UUU)

Phenylalanine (UUC)

Leucine (UUA)

Leucine (UUG)

Leucine (CUU)

Leucine (CUC)

Leucine (CUA)

Leucine (CUG)

Isoleucine (AUU)

Isoleucine (AUC)

Isoleucine (AUA)

Methionine;Start (AUG)

Valine (GUU)

Valine (GUC)

Valine (GUA)

Valine (GUG)

Serine (UCU)

Serine (UCC)

Serine (UCA)

Serine (UCG)

Proline (CCU)

Proline (CCC)

Proline (CCA)

Proline (CCG)

Threonine (ACU)

Threonine (ACC)

Threonine (ACA)

Threonine (ACG)

Alanine (GCU)

Alanine (GCC)

Alanine (GCA)

Alanine (GCG)

Tyrosine (UAU)

Tyrosine (UAC)

Stop (UAA)

Stop (UAG)

Histadine (CAU)

Histadine (CAC)

Glutamine (CAA)

Glutamine (CAG)

Asparagine (AAU)

Asparagine (AAC)

Lysine (AAA)

Lysine (AAG)

Aspartate (GAU)

Aspartate (GAC)

Glutamate (GAA)

Glutamate (GAG)

Cysteine (UGU)

Cysteine (UGC)

Stop (UGA)

Tryptophan (UGG)

Arginine (CGU)

Arginine (CGC)

Arginine (CGA)

Arginine (CGG)

Serine (AGU)

Serine (AGC)

Arginine (AGA)

Arginine (AGG)

Glycine (GGU)

Glycine (GGC)Glycine (GGC)

Glycine (GGA)

Glycine (GGG)

:The Genetic Code:The Genetic Code

Page 67: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• Some codons do not code for amino acids; they provide instructions for making the protein.

• More than one codon can code for the same amino acid.

• However, for any one codon, there can be only one amino acid.

The Genetic CodeThe Genetic Code

Page 68: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• All organisms use the same genetic code.

• This provides evidence that all life on Earth evolved from a common origin.

The Genetic CodeThe Genetic Code

Page 69: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

Translation: From mRNA to ProteinTranslation: From mRNA to Protein

• The process of converting the information in a sequence of nitrogenous bases in mRNA into a sequence of amino acids in protein is known as translation.

• Translation takes place at the ribosomes in the cytoplasm.

• In prokaryotic cells, which have no nucleus, the mRNA is made in the cytoplasm.

Page 70: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

Translation: From mRNA to ProteinTranslation: From mRNA to Protein

• In eukaryotic cells, mRNA is made in the nucleus and travels to the cytoplasm.

• In cytoplasm, a ribosome attaches to the strand of mRNA like a clothespin clamped onto a clothesline.

Page 71: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• For proteins to be built, the 20 different amino acids dissolved in the cytoplasm must be brought to the ribosomes.

• This is the role of transfer RNA.

The role of transfer RNAThe role of transfer RNA

Page 72: EOI PRACTICE A class designed an experiment to test the effects of nitrogen fertilizer on grass growth. The steps for the experiment are shown below but

• Each tRNA molecule attaches to only one type of amino acid.

Amino acid

Chain of RNA nucleotides

Transfer RNA molecule

Anticondon

The role of transfer RNAThe role of transfer RNA

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• As translation begins, a ribosome attaches to the starting end of the mRNA strand. Then, tRNA molecules, each carrying a specific amino acid, approach the ribosome.

• When a tRNA anticodon pairs with the first mRNA codon, the two molecules temporarily join together.

The role of transfer RNAThe role of transfer RNA

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The role of transfer RNAThe role of transfer RNA

Ribosome

mRNA codon

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• Usually, the first codon on mRNA is AUG, which codes for the amino acid methionine.

• AUG signals the start of protein synthesis.

• When this signal is given, the ribosome slides along the mRNA to the next codon.

The role of transfer RNAThe role of transfer RNA

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tRNA anticodon

Methionine

The role of transfer RNAThe role of transfer RNA

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• A new tRNA molecule carrying an amino acid pairs with the second mRNA codon.

Alanine

The role of transfer RNAThe role of transfer RNA

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• The amino acids are joined when a peptide bond is formed between them.

AlanineMethionine

Peptide bond

The role of transfer RNAThe role of transfer RNA

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• A chain of amino acids is formed until the stop codon is reached on the mRNA strand.

Stop codon

The role of transfer RNAThe role of transfer RNA

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Translation

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What are the three chemical differences between RNA and DNA?

Question 1

Answer

RNA consists of a single strand of nucleotides whereas DNA is a double strand. RNA contains ribose as its sugar and DNA contains deoxyribose as its sugar. Uracil in RNA replaces thymine in DNA as the nitrogenous base.

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What is the role of rRNA in protein synthesis?

Question 2

Answer

Ribosomal RNA binds to messenger RNA and assembles the amino acids in the order needed for the protein to be synthesized.

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Which regions of the mRNA travel to the ribosome; introns, exons, or both?

Question 3

Answer

Only exons, which contain coding information, travel to the ribosome. Introns, noncoding nucleotide sequences, do not travel to the ribosome.

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What is an anticodon, and what does it represent?

Question 4

Answer

An anticodon is a sequence of three nucleotides on the tRNA molecule that binds to a codon of the mRNA strand.

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EOI PracticeThe picture shows a cross-section of a living structure

There are many different types of plant cells in the structure. Some cells are involved in photosynthesis, and others are involved in the transport of materials.

What is the correct classification for the living structure?

A) tissue B) organ systems

C) organ D) organism

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EOI PracticeThe picture shows a cross-section of a living structure

There are many different types of plant cells in the structure. Some cells are involved in photosynthesis, and others are involved in the transport of materials.

What is the correct classification for the living structure?

A) tissue B) organ systems

C) organ D) organism

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EOI PracticeA certain disease in humans is a recessive trait (n) while the normal condition is dominant (N). The Punnett square below shows the genotypes for a couple that wants to have a child.

What is the probability that the couple’s child will inherit the disease?

A) 0% B) 25%

C) 50% D) 100%

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EOI PracticeA certain disease in humans is a recessive trait (n) while the normal condition is dominant (N). The Punnett square below shows the genotypes for a couple that wants to have a child.

What is the probability that the couple’s child will inherit the disease?

A) 0% B) 25%

C) 50% D) 100%

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EOI Practice

Which statement best describes the outcome of the process in the diagram?

A) four identical daughter cells

B) four sex cells, two male and two female

C) four daughter cells, each with a different number of chromosomes

D) four sex cells, each with a different combination of genetic material

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EOI Practice

Which statement best describes the outcome of the process in the diagram?

A) four identical daughter cells

B) four sex cells, two male and two female

C) four daughter cells, each with a different number of chromosomes

D) four sex cells, each with a different combination of genetic material

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EOI PracticeA certain species of wasp is the only pollinator for a certain species of plant. The plant is the only source of food and shelter for the wasp. The graph shows the plant and wasp population trends.

Which will most likely happen to the plant population if an introduced disease kills all the wasps in a short period of time?

A) It will grow more slowly.

B) It will become extinct.

C) It will be pollinated by other species of wasps.

D) It will change into a different species of plant.

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EOI PracticeA certain species of wasp is the only pollinator for a certain species of plant. The plant is the only source of food and shelter for the wasp. The graph shows the plant and wasp population trends.

Which will most likely happen to the plant population if an introduced disease kills all the wasps in a short period of time?

A) It will grow more slowly.

B) It will become extinct.

C) It will be pollinated by other species of wasps.

D) It will change into a different species of plant.

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EOI PracticeJulia is monitoring the growth of a bacterial population in a 50-mL flask.

According to the graph, the population reached the carrying capacity of its environment in about

A) 10 hours. B) 15 hours.

C) 20 hours. D) 30 hours.

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EOI PracticeJulia is monitoring the growth of a bacterial population in a 50-mL flask.

According to the graph, the population reached the carrying capacity of its environment in about

A) 10 hours. B) 15 hours.

C) 20 hours. D) 30 hours.

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EOI PracticeIn an ecosystem, energy from the sun is converted and transferred to all members of the biotic community.

At which level of organization is solar energy converted by photosynthesis?

A) cells

B) organs

C) organisms

D) populations

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EOI PracticeIn an ecosystem, energy from the sun is converted and transferred to all members of the biotic community.

At which level of organization is solar energy converted by photosynthesis?

A) cells

B) organs

C) organisms

D) populations

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• Categorize the different kinds of mutations that can occur in DNA.

Section Objectives:

• Compare the effects of different kinds of mutations on cells and organisms.

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• Organisms have evolved many ways to protect their DNA from changes.

Mutations

• In spite of these mechanisms, however, changes in the DNA occasionally do occur.

• Any change in DNA sequence is called a mutation.

• Mutations can be caused by errors in replication, transcription, cell division, or by external agents.

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• Mutations can affect the reproductive cells of an organism by changing the sequence of nucleotides within a gene in a sperm or an egg cell.

Mutations in reproductive cells

• If this cell takes part in fertilization, the altered gene would become part of the genetic makeup of the offspring.

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Mutations in reproductive cells

• The mutation may produce a new trait or it may result in a protein that does not work correctly.

• Sometimes, the mutation results in a protein that is nonfunctional, and the embryo may not survive.

• In some rare cases a gene mutation may have positive effects.

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• What happens if powerful radiation, such as gamma radiation, hits the DNA of a nonreproductive cell, a cell of the body such as in skin, muscle, or bone?

• If the cell’s DNA is changed, this mutation would not be passed on to offspring.

• However, the mutation may cause problems for the individual.

Mutations in body cells

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Mutations in body cells

• Damage to a gene may impair the function of the cell.

• When that cell divides, the new cells also will have the same mutation.

• Some mutations of DNA in body cells affect genes that control cell division.

• This can result in the cells growing and dividing rapidly, producing cancer.

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• A point mutation is a change in a single base pair in DNA.

• A change in a single nitrogenous base can change the entire structure of a protein because a change in a single amino acid can affect the shape of the protein.

The effects of point mutations

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The effects of point mutations

Normal

Point mutation

mRNA

ProteinStop

Stop

mRNA

Protein

Replace G with A

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Frameshift mutations

• What would happen if a single base were lost from a DNA strand?

• This new sequence with the deleted base would be transcribed into mRNA. But then, the mRNA would be out of position by one base.

• As a result, every codon after the deleted base would be different.

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Frameshift mutations

mRNA

Protein

Frameshift mutation

Deletion of U

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Frameshift mutations

• This mutation would cause nearly every amino acid in the protein after the deletion to be changed.

• A mutation in which a single base is added or deleted from DNA is called a frameshift mutation because it shifts the reading of codons by one base.

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• Changes may occur in chromosomes as well as in genes.

• Alterations to chromosomes may occur in a variety of ways.

• Structural changes in chromosomes are called chromosomal mutations.

Chromosomal Alterations

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• Chromosomal mutations occur in all living organisms, but they are especially common in plants.

• Few chromosomal mutations are passed on to the next generation because the zygote usually dies.

Chromosomal Alterations

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• In cases where the zygote lives and develops, the mature organism is often sterile and thus incapable of producing offspring.

• When a part of a chromosome is left out, a deletion occurs.

Deletion

A B C D E F G H A B C E F G H

Chromosomal Alterations

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• When part of a chromatid breaks off and attaches to its sister chromatid, an insertion occurs.

• The result is a duplication of genes on the same chromosome.

Insertion

A B C D E F G H A B C B C D E F G H

Chromosomal Alterations

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• When part of a chromosome breaks off and reattaches backwards, an inversion occurs.

Inversion

A B C D E F G H A D C B E F G H

Chromosomal Alterations

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• When part of one chromosome breaks off and is added to a different chromosome, a translocation occurs.

A B E FDCBX AWC HGGE HD F

W X Y Z Y ZTranslocation

Chromosomal Alterations

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• Some mutations seem to just happen, perhaps as a mistake in base pairing during DNA replication.

• These mutations are said to be spontaneous.

• However, many mutations are caused by factors in the environment.

Causes of Mutations

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• Any agent that can cause a change in DNA is called a mutagen.

• Mutagens include radiation, chemicals, and even high temperatures.

• Forms of radiation, such as X rays, cosmic rays, ultraviolet light, and nuclear radiation, are dangerous mutagens because the energy they contain can damage or break apart DNA.

Causes of Mutations

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Causes of Mutations• The breaking and reforming of a double-

stranded DNA molecule can result in deletions.

• Chemical mutagens include dioxins, asbestos, benzene, and formaldehyde, substances that are commonly found in buildings and in the environment.

• Chemical mutagens usually cause substitution mutations.

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Repairing DNA• Repair mechanisms that fix mutations in cells

have evolved.

• Enzymes proofread the DNA and replace incorrect nucleotides with correct nucleotides.

• These repair mechanisms work extremely well, but they are not perfect.

• The greater the exposure to a mutagen such as UV light, the more likely is the chance that a mistake will not be corrected.

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Any change in DNA sequences is called a _______.

Question 1

D. translation

C. transcription

B. mutation

A. replication

The answer is B.

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Which is more serious, a point mutation or a frameshift mutation? Why?

Question 2

Answer

A frameshift mutation is more serious than a point mutation because it disrupts more codons than a point mutation.

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Why are chromosomal mutations rarely passed on to the next generation?

Question 3

Answer

Few chromosomal changes are passed on to the next generation because the zygote usually dies. If the zygote survives, it is often sterile and incapable of producing offspring.

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Question 1

How does DNA control the structures and functions of a cell?

Answer

DNA determines the structure of proteins. Some proteins become important cell structures. Other proteins, such as enzymes, control chemical reactions that perform key life functions.

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Question 2The process through which the order of bases in messenger RNA codes for the order of amino acids in a protein is:

D. point mutation

C. replication

B. translation

A. transcription

The answer is B.

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Question 3

Why would scientists use nucleotide sequences to identify bodies of crime victims?

Answer

In comparing nucleotide sequences in the DNA of a crime victim with nucleotide sequences from a possible close relative of the crime victim, scientists can determine if the two are related.

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Question 4

What happens when a stop codon is reached during translation?

Answer

When a stop codon is reached, translation ends and the amino acid strand is released from the ribosome.

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Question 5

A ________ bond forms between adjacent amino acids during translation.

D. peptide

C. hydrogen

B. phosphate

A. nucleotide

The answer is D.

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Question 6

Why is DNA replication important to cell division?

Answer

Without DNA replication, new cells would have only half the DNA of their parents. Species could not survive and individuals could not grow or reproduce successfully.

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Question 8At the beginning and end of replication, which of the following are instrumental in breaking and bonding the hydrogen bonds between bases?

D. enzymes

C. nucleotides

B. purines

A. pyrimidines

The answer is D.

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Question 9

What is the role of mRNA in protein synthesis?

Answer

The messenger RNA acts as a genetic message, providing the complete information, in sequences of codons, for the building of a protein.

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End of Chapter 11 Show

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C.

A.

B.