Methods in Enzymology

Volume 11 7

Enzyme StructurePart J

C. H . W . Hirs

Serge N. Timasheff

CONTRIBUTORS TO VOLUME 117 Vi i

PREFACE i X

VOLUMES IN SERIES X i

Section I . Size, Shape, and Polydispersity of Macromolecule s

1 . Characterization of Macromolecules by Sedimen- ROBERT J . POLLET

3

tation Equilibrium in the Air-Turbine Ultracen-

trifuge

2 . Measurement of Changes of Hydrodynamic Prop- ROBERT W . OBERFELDER ,

erties by Sedimentation

THOMAS G . CONSLER, AND

JAMES C . LEE

27

3 . Determination of Protein Molecular Weight in JACQUELINE A . REYNOLD S

Complexes with Detergent without Knowledge AND DARRELL R . MCCASLIN 4 1of Binding

4. Calculation of Partial Specific Volumes of Proteins V . PRAKASH AND

in 8 M Urea Solution

SERGE N . TIMASHEFF

5 3

5 . Calculation of the Partial Specific Volume of Pro- T . ARAKAWA AN D

teins in Concentrated Salt and Amino Acid So- SERGE N . TIMASHEFF

60

lution s

6 . Molecular Weight Determinations from Radiation J . T . HARMON ,

Inactivation

T . B . NIELSEN, AN D

E . S . KEMPNER

65

7. Measurement of Molecular Weights of Elongated BRUNO H . ZIMM

94

Molecules by Viscoelastometry

8. Measurement of Hydrodynamic Properties of Ac- LYNDAL K . HESTERBERG AN D

tive Enzyme by Sedimentation

JAMES C . LEE

9 7

9 . Computer-Controlled Scanning Gel Chromatog- LAWRENCE C . DAVIS ,

raphy

THEODORE J . SOCOLOFSKY ,

AND GARY A . RADKE

11 6

10. Hydrodynamic Characterization of Random Coil PHIL G . SQUIRE

142Polymers by Size Exclusion Chromatograph y

11 . Structural Interpretation of Hydrodynamic Mea- THOMAS F . KUMOSINSKI AN D

surements of Proteins in Solution through Cor- HELMUT PESSEN

15 4relations with X-Ray Data

12 . The Use of Covolume in the Estimation of Protein LAWRENCE W . NICHOL AND

Axial Ratios

DONALD J . WINZOR

18 2

13 . Application of Transient Electric Birefringence to PAUL J . HAGERMAN

198

the Study of Biopolymer Structure

14 . Measurements of Protein Hydration by Various HELMUT PESSEN AN D

Techniques

THOMAS F . KUMOSINSKI

21 9

15 . Correlation Function Profile Analysis of Polydis- B . CHU, J . R . FORD, AN D

perse Macromolecular Solutions and Colloidal H . S . DHADWAL

25 6

Suspension s

Section II . Interaction of Macromolecules with Ligands and Linkages

16. Nonlinear Least-Squares Analysis

MICHAEL L . JOHNSON AN D

SUSAN G . FRASIER

30 1

17 . Ligand Binding to Proteins by Equilibrium Gel HIROsHI KIDO ,

Penetration

ALBERTO VITA, AN D

B . L . HORECKER

342

18 . Measurement of Protein-Ligand Interactions by Jost MANUEL ANDREU

346

Gel Chromatograph y

19 . Ultrafiltration in Ligand-Binding Studies

ALKIS J . SOPHIANOPOULOS AND

JUDITH A . SOPHIANOPOULOS 35 4

20 . Protein Chromatography on Hydroxyapatite MARINA J . GORBUNOFF

370

Column s

21 . Measurement of Ligand-Protein Interaction by ROBERT W . OBERFELDER AN D

Electrophoretic and Spectroscopic Techniques JAMES C . LEE

38 1

22 . Measurement of Ligand Binding to Proteins by LARRY D . WARD

400

Fluorescence Spectroscopy

23 . Measurement of Metal-Ligand Distances by ROBERT A . SCOTT

41 4

EXAF S

24 . Velocity Sedimentation Study of Ligand-Induced GEORGE C . NA AN D

Protein Self Association

SERGE N . TIMASHEFF

459

25 . Measurement and Analysis of Ligand-Binding Iso- GEORGE C . NA AN D

therms Linked to Protein Self-Associations

SERGE N . TIMASHEFF

496

AUTHOR INDEX 52 1

SUBJECT INDEX 535