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ENVIRONMENTAL PROTECTION THROUGH CONSERVATION AND REMEDIATION

Contact details

Name Environmental Protection through Conservation and Remediation

Acronym ENVIRONMENTALIA

Logo

Site

Address 76, Victoriei Str., 430122, Baia Mare, Romania

Faculty Department

Faculty of Sciences Department of Chemistry and Biology

Telephone +40 262 276059

Fax +40 262 275368

Director Assoc. Prof. Dr. Marian Jelea

e-mail mjelea@yahoo.com , marian.jelea@ubm.ro , +40 740 341280

Areas of expertise

Assessment of biodiversity in natural and anthropogenic ecosystems, the conservation or remediation of degraded lands in perspective by promoting restoration of natural habitats. Identifying, testing and application of combinations of species including microorganisms (bacteria, cianoficee), fungi and plants able to remedy degraded soils and stimulate the installation of natural habitats. Recovery of copper through bio-technological procedures from the low-grade ores. Applied research on tissue culture and plants multiply "in vitro".

Team and key skills

Assoc. Prof. Dr. Marian Jelea Ed.: Faculty of Biology, Univ. of Bucharest - 1983. Prof. exp.: Mining R&D Institute Baia Mare (Researcher II, 1988-’98); UBM-UTCN (1998-present). Holder of Microbiology disciplines. Skills and comp.: Recovery of copper through bio-technological procedures from the low-grade ores - elaboration of technology from Roşia Poieni. Protection of the environment in the affected areas by mining industry. Ecological reconstruction of the soil affected by mining industry. Research studies for native vegetation installed in the acidic mine waste areas. Assoc. Prof. Dr. Monica Marian Ed.: Graduated from the Faculty of Biology (Univ. UBB). Prof. exp.: Holder Northern University in 1993, holder of plant biology disciplines. Skills and comp.: Skills and research projects in the fields of flora and vegetation assessment of areas protected land and polluted / contaminated with heavy metals multiplication "in vitro" Assist. Prof. Dr. Stela-Gabriela Jelea Ed.: Faculty of Biology, Univ. of Bucharest - 1984. Prof. exp.: UBM-UTCN (1994-present). Skills and comp.: Research in histology, animal physiology; toxicology, human nutrition.

Infrastructure

UNICAM 5625 UV/VIS/NIR Spectrophotometer; PERKIN ELMER 3110 Atomic Absorption Spectrometer (Lampa cathode Au/Ag, Na/K, As, Co/Cu/Fe/Mn/Ni, Ca/Mg/Cu/Zn/Cr/Fe); Microcalorimeter - Thermometric AB 2277-C3-Analysentechnik outdoor thermostat; Microscope - HUND H 500 AFI 50 with fluorescentacatod; Centrifugal BIOFUGE cooled -28 RS; BIOFUGE-PICO; Granulometric analysis device AS BASIC 200V/50Hz, with a set of site; pH - portable meter 323 A, WTW - with accessories; Adjustable pipettes 2-20 µl, 20-200 µl, 200-1000 µl; UNITRON incubator with shaking and cooling pan with accessories; Microbiological hood; Dispensette-BRAND 2-10 ml; 5-50 ml; 100 ml pieces;.Shaker for tubes; Apparatus for determination of cyanide with 5 seats – BEHR –Labor – Technik; Analytical balance - SARTORIUS-BP 210S; Ovens thermoregulation - WTC BINDER 2 pieces; Portable oxy- meter Oxi-323 A,

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WTW, with accessories; MASTERCLAVE culture media preparator; Spectrophotometer PG Instruments T70, UV/VIS, PG; Microbiological incubator thermostat LE-549 SCILAB Instrument Ltd; Microbiological incubator thermostat with turntable mixing ,HEIDOLPH, Titramax 1000; Orbital shaker HEIDOLPH, Rotamax 120; Microbiological incubator with forced ventilation; Horizontal laminar flow hood with HEPA filter; Laboratory microcentrifuge EBA 20 with rotor; Binocular biological microscope MBL 2000 A. KRUSS Optronic GmbH; Trinocular biological microscope MBL 2100 A. KRUSS Optronic GmbH; Precision balance WPS 210/C/2 RADWAG; Orbital shaker TS-500D SCILAB INSTRUMENT Ltd; Automatic colony counter FUNKE GERBER, COLONYSTAR; Mixer mixing samples Velp, VORTEX CLASIC; pH-meter pH/REDOX/EC/TDS/NaCl/Temperature Hi 255 HANNA INSTRUMENT; Termohigrometer; Refrigerator biological samples, ARCTIC FB 245; Digital reception and microscopic imaging - digital camera NIKON COOLPIX P5000, zoom optic 3,5x, 12,0 Megapixeli; Retsch mortar calcination RM100; Oven Nabertherm B180, Centurion Scientific Laboratory Centrifuge K240, BS3030 Raypa sand bath; Ceramic hob Scott Intsruments SLK2, Solvent extraction SER148 Velp Scientific; Microbiological hood Aquaria Bio Activa 120; Microbiological hood Bioquelle Microflow ABS; Stereomicroscope Stemi 1000 Zeiss, Atomic Absorbtion Spectrometer Perkin Elmer 800 Aanalyst; Chamber of vegetation Sanyo MLR 351H; Thermo Scientific ultrapure water system EasyPure RODI; Pomegranate disaggregation system MWS2 evidence Berghof microwave system; Orbital stirrer Heidolph Unimax 1010; Heidolph Incubator Inkubator 1000; Centrifuge Hettich EBA20; Funke Gerber WB436A water bath; Homogenized Heidolph RZR 2021; InoLab WTW pH meter pH 730; Kern ABJ analytical Balance; Balance Kern EG technique; Large capacity autoclave; Stereomicroscopes Kruss; Colony counter.

Development strategy

High quality research by: - Interdisciplinary collaboration in identifying a range of possible topics specific knowledge capitalization multiple fields. - Also follow a theme adapted industrial area is located Baia Mare, in the context of targeting priorities contaminated land remediation and conservation of natural reserves of flora and fauna.

Representative projects in the last 5 years

Coordinating: Monitoring action microbiota in order to use them in the soil remediation ponds (2007-2010). Partners in Agriculture Research Station orchard development, University of Oradea. Funding: Ministry of Education and Research through the PNII. A project aimed at identifying biological pathways using a combination of plants and fungi remediation tailings contaminated with heavy metals. "We can refund flowers borrowed from our children?" In situ and ex situ protection of endangered plant species Ţibleşului Mountains, Valley Lăpuş, Someş Valley and Ridge Codrului (2008-2009). Funding Environment Fund Administration. Developing educational methods for protecting endangered plant species. Conservation of biodiversity and ecological reconstruction of the lower basin of the River Tour - Adrian pond (protected area - 400h) (2007-2008) Funded by AFM. Inventory and mapping activities of species and habitats of EU conservation interest of a Natura 2000 site. Evolution of installation of iron and sulphur oxidizing bacteria in the sulphidic mine wastes and their influence in generating acid mine drainage. (2007-2008). GRANT CNCSIS, Type project A Cod 450, issue no.1, contract no. 3GR/11.05.2007. The study is a tool for those interested in an approach suitable solutions to reduce / stop the acid mine drainage phenomena and ecological remediation of tailings deposits, which can increase the success rate of the techniques applied and / or lead to lower costs (http://194.102.64.7/GranturiFinalizate/faces/Projects/ProjectDetails.jsp). Member of the following projects: Rehabilitation of tailings by applying amendments and cultivation of plant species with high adaptability to heavy metal content - RIVAM (2008-2011). Funding: CNCSIS by PNCDI 2 – Partnerships. Testing activities of fungal strains with high capacity absorption and adsorption of heavy metals. Bioaccumulation of heavy metals in soil-plant-human chain - BIOMEG. (2008-2011). CNCSIS by PNCDI 2 – Partnerships. Identification and testing activities of various vegetables and fruits in terms of accumulation of heavy metals and of how heavy metals accumulate in the food chain. Development of a zeolite-based biophysical micro-plant species on eco-remediation industry tailings from the preparation of gold-silver ore (2008-2011). Funding: CNCSIS in the PNCDI 2 - bilateral projects. Testing combinations of bacteria / fungi used in biological remediation of tailings dams. Interdisciplinary research on soil-plant correlation, establishing transfer factors for historical anthropogenic pollution areas (2008-2011). Grant CNMP. Programme 4. Partnerships in priority areas, contract 52157/2008. The project realized a study on soil pollution from industrial zone establishes correlations between soil and transfer of pollutants into the vegetables and then, through their use in the human body. (www.ubm.ro/sites/CISPPA_2008/cisppa_2008.html) Education in separation and identification of organic xenobiotics in Environmental and food product samples. (2008-2009). Funding: European Union within CEEPUS (Central European Exchange Program for Universities Study, CEEPUS II LP - 0401-2008-2009 in the field of Analytical Chemistry Applied to Environment Monitoring and Protection). Teaching and research activities in the field of food and environmental contaminants. Prevent and eliminate environmental impacts using geosynthetic materials (2007-2008). GRANT CNCSIS. Type project A, Cod 446, no.3, GR/11.05.2007. The project aims at studying the effects of mining waste on the environment and prevention of these effects by using geosynthetic membranes. Project WWF for Nature International - Danube - Carpathian Programme: Priority forest, sub-alpine and alpine

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habitats in Romania, (2007). LIFE05NAT/RO/00176, Project number EN 00081, 2007. Identification and mapping of priority habitats Gutâi. WWF Project One Europe More Nature programs in Romania, Baseline study, Monitoring biodiversity on grassland in Maramureş. (2007). Project number: 8W006.1, 2007. Monitoring grassland to identify the impact that it has on biodiversity intensive grazing. Development of methods for stabilization and rehabilitation of soils polluted with heavy metals using zeolite resources (2006-2008). GRANT CNCSIS. Type project A, Cod 138, issue no.1, contract no. 5GR/09.05.2006. The effect of magnetic anomalies on terrestrial biodiversity and preservation of extinct forms of life. (2006-2007). PNCDI project developed by Babeş-Bolyai University in partnership with University of Baia Mare. Overall assessment activities flora of the Czech Republic and Ukraine. Together for rehabilitation Ierului Valley (2005-2008). Funding: PHARE RO 2005/017-536.01.01.0124. Assessment activities flora and habitats in Natura 2000 sites.

Significant results

Papers Published In ISI Reviews 1. M. Marian, A. Peter, L. Mihaly-Cozmuta, E. Bakatula, „Increased Survival Chances of the Species Quercus

petraea in Terms of Pollution with Cd and Cu by Using Microbiota-Bentonite Systems”, Carpathian Journal of Earth and Environmental Sciences, Vol. 7, No. 1, February 2012, pp. 231 – 237.

2. A. Lucaciu, C. Motoc, M. Jelea, S.G. Jelea, „Survey of heavy metal deposition in Romania: Transylvania plateau and western Carpathians mountains”. University Politehnica of Bucharest, Scientific Bulletin, Series A - Applied Mathematics and Physics, Vol. 72, Iss. 2, 2010, pp. 171-178.

3. S. G. Jelea, M. Jelea, „Effects of subacute treatment with lead acetate on the mineral constituents from femur and tibia”. Carpathian Journal of Earth and Environmental Sciences, Vol. 4, No. 1, 2009, pp. 61-68.

4. V. Oros, M. Coman, M. Marian, L. Mihaly, A. Mihaly, „Preliminary investication aimed to ecological reclaiming by phytoremediation of a large flotation tailing dump in Baia Mare mining area”, Environmental Engineering and Management Journal, Vol 8, No. 4, July/August 2009, pp. 915-922.

5. L. Mihaly-Cozmuta, C. Varga, M. Marian, A. Mihaly-Cozmuta, T. Hanglani, „Applying artificial networks in the modelling of copper recovery process by ionic exchange in aquous solutions”, Revista de Chimie, (59) No. 7, 2008, pp. 766-772.

6. S. G. Jelea, M. Jelea, „Effects of polluting substances on risk categori molten and agglomeration workers from Romplumb Society Baia Mare”, Carpathian Journal of Earth and Environmental Sciences, Vol. 2, No. 1, 2007, pp. 51-57.

7. M. Jelea, S. G. Jelea, Z. M. Kovacs, D. E. Gheţa, „Research concerning the oxidation degree of the sulphidic tailings from the Novaţ tailings storage facility”, Carpathian Journal of Earth and Environmental Sciences, Vol. 2, No. 2, 2007, pp. 45-55.

Papers Published in B+ (BDI) Reviews

1. S. G. Jelea, M. Jelea, R. Hotea, Z. Vosgan, „Assessment of the evolution of anthropometric indicators in students with sports program” Studia Universitatis “Vasile Goldiş”, Life Sciences Series, Vol. 22, Issue 3, 2012, pp. 403-409.

2. M. Marian, A. Peter, L. Mihalescu, Z. Vosgan, G. Matei, „Allelopatic Potential of Asarum europaeum toward Lycopersicon esculentum”, Analele Universităţii din Oradea - Fascicula Biologie, Tom. XVIII, Issue: 1, 2011, pp. 39-44.

3. Z. Vosgan, R. Vidican, S.G. Jelea, M. Marian, L. Mihalescu, O. Mare Rosca, „Characterization of a typical districambosol soil from the Gutai Mountains – North of Romania”, Bulletin of University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Agriculture, 68 (1), 2011, pp. 384-387.

4. Z. Vosgan, R. Vidican, M. Marian, L. Mihalescu, S. G. Jelea, O. Mare Rosca, „Researches regarding the vegetation of Surdesti village, Maramures Country”, Bulletin of University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Agriculture, 68 (1): 2011, p. 436.

5. S. G. Jelea, „Study concerning the effects of pollution on the staff worker”, Studia Universitatis “Vasile Goldiş”, Life Sciences Series, Vol. 20 (1), 2010, pp. 69-74.

6. S. G. Jelea, M. Jelea, „Pulmonary tuberculosis incidence analyssis in the adults population of Maramures county, during 2006-2007”, Annals of the Romanian Society for Cell Biology, Vol. XV, Issue 1, 2010, pp. 231-235.

7. S. G. Jelea, M. Jelea, R. Hotea, „Asssessing the health condition of children with sports program by anthropometric nutrition”, Studia Universitatis “Vasile Goldiş”, Life Sciences Series, Vol. 20, August 2010, pp. 27-33.

8. M. Marian, C. Nicula, L. Mihaly-Cozmuta, A. Peter, A. Mihaly-Cozmuta, „Participation Of The Indigenous vs. Alien Herbaceous Species To The Constitution Of Vegetal Layer On The Bozanta Mare Tailing Ponds”, Analele Universitatii din Oradea, Tom XVII/1, 2010, pp. 134-141.

9. C. Nicula, M. Marian, L. Mihaly-Cozmuta, A. Peter, A. Mihaly-Cozmuta, „Adaptive Mechanisms Of Phaseolus vulgaris And Zea mays Seeds Grown In Agrocenoses Prone To Pollution With Heavy Metals”, Analele Universitatii din Oradea, Tom XVII/1, 2010, pp. 152-157.

10. G. Oprea, A. Michnea, C. Mihali, M. Senila, C. Roman, S., Jelea, C. Butean, C. Barz, „Arsenic and Antimony Content in Soil and Plants from Baia Mare Area, Romania”. American Journal of Environmental Sciences 6 (1), 2010, pp. 33-40.

11. S. G. Jelea, M. Jelea, „Assessing the Main Anthropometric Variable with Teenagers from Maramureş Country”, Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture, 66(2), 2009, pp. 105-109.

12. S. G. Jelea, M. Jelea, „Prevalence of Some Severe Diseases Diagnosed to 0-7 Years Old Children, in the Baia Mare Region”, Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture, 66(2), 2009, p.

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545. 13. S. G. Jelea, M. Jelea, „Study of body mass index wirh teenagers from Maramures country”. Annals. Food Science

and Tecnology. Vol 10, Issue 1, 2009, pp. 335-337. 14. M. Marian, L. Mihaly-Cozmuta, C. Varga, A. Mihaly-Cozmuta, E. Nour, „Vegetation Dynamics Depending on

Ecological Particularities of Bozanta Mare (Maramures County-Romania) Tailing Pound: Case Study”, American Journal of Environmental Sciences 5(1), 2009, pp. 116-123.

15. C. Varga, M. Marian, A. Peter, D. Boltea, L. Mihaly-Cozmuta, E. Nour, „Strategies of heavy metal uptake by Phaseolus vulgaris seeds growing in metalliferous and non metalliferous areas”, Studia Universitatis, Babes Bolyai, Chemia, LIV, 3, 2009, pp. 223-234.

16. M. Marian, C. Varga, L. Mihaly-Cozmuta, A. Mihaly-Cozmuta, „Evaluation of the Phytoremediation Potential of the Salix caprea in Tailing Ponds”, Analele Universitatii din Oradea, Tom XIV. 2009, pp. 141-149.

17. M. Marian, C. Varga, L. Mihaly-Cozmuta, A. Mihaly-Cozmuta, A. Peter, D. Boltea, „Evaluation of the Phytoremediation Potential of the Populus tremula in Tailing Ponds”, Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture, Cluj Napoca. 66/2, 2009, pp.124-131.

18. M. Jelea, S. G. Jelea, „Large-scale Experiments for Copper Extraction by Bacterial Heap Leaching of the Chalcopyrite Predominant Ore of Roşia Poieni”, Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca - Animal Sciences and Biotechnologies, 65(1-2), 2008, pp. 330-335.

19. S. G. Jelea, „Biochemical and structural changes in the liver at prepubescent rats treated with lead acetate”. Buletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Veterinary Medicine, 65 (2), 2008, .pp. 16-19.

20. S. G. Jelea, M. Jelea, „Incidenţa tumorilor colorectale diagnosticate, în perioada 2000-2004, la Spitalul Judeţean din Satu Mare”, Annals of the Romanian Society for Cell Biology, Vol. XIII, Issue 1, 2008, pp. 71-75.

21. M. Marian, L. Mihaly-Cozmuta, C. Varga, A. Mihaly-Cozmuta, „The ecology of the phytocoeno-genesis process in the tailing ponds in Maramures county”, Natura Montenegrina, nr. 7 (3), 2008, pp. 41-55.

22. M. Marian, C. Varga, L. Mihaly-Cozmuta, A. Mihaly Cozmuta, L. Mihalescu, O. Rosca Mare, C. Blidar, „Variance analysis on different trees species depending on soil type – uncontaminated and heavy metals contaminated ones”, Analele Universitatii din Oradea, Tom XIV, 2008, pp. 41-46.

The offer addressed to the economic environment

Research & development in core areas

- Evaluation of species of flora / fauna, microorganisms, fungi, from natural and anthropogenic habitats in order to protect themselves or to remedy environmental.

Research & development in applied fields

- Identifying biological methods based on the use of complex organisms able to reduce contamination of soil / water and facilitate the restoration of ecosystems; - Identification of plant extracts alelopatic greenhouse (natural pesticides) in weed control and phytopathogenic; - In vitro multiplication of species of plants for remediation and / or cultivation; - The analysis, monitoring and diminishing of the effects produced by the polluting factors from industry; - Evolution of installation of iron and sulphur oxidizing bacteria in the sulphidic mine wastes and their influence in generating acid mine drainage; - Research studies for native vegetation installed in the acidic mine waste areas.

Consulting - Structure and function in natural ecosystems and to restore contaminated their. - Growing plants in different conditions of land polluted and / or contaminated. - Evaluation of medical resources in the spontaneous flora, possible methods for obtaining the active principles of tissue culture. - Biological assessment of contaminated land. - Combinations of organisms with potential decontaminant. - Impact assessment polluting agents on health of population. - Epidemiological surveys in public health.

Applied engineering services - Recovery of copper through biotechnological procedures from the low-grade ores. - Reducing acid mine drainage (AMD) phenomena through passive methods.

Training - Structure and function in natural ecosystems and to restore contaminated their. - Growing plants in different conditions of land polluted and / or contaminated. - Evaluation of medical resources in the spontaneous flora, possible methods for obtaining the active principles of tissue culture. - Biological assessment of contaminated land. - Occupational toxins, the relationship between chemical structure and toxic action, dose-effect relationships and dose-response relationships. - Ecological restoration of degraded mining lands acid extraction (mining waste dumps and tailings).

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Fig. 1. Research on Environmental Microbiology Laboratory

Fig 2. Biotechnology Research Laboratory (tissue cultures)

Fig. 3. Research on Environmental Microbiology Laboratory

Fig. 4. Monitoring of vegetation on mining dumps

Fig. 5. Monitoring the acid mine drainage phenomena (AMD)