Entering the lab (A fresh beginning)

Andrei Leitão

Types of pipets

Pasteur Transfer Volumetric Serological Mohr

To contain (TC) To deliver (TD)

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Types of pippetors Micropippetor

Manual Electronic Robotics

Electronic pippetor

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Flasks Beaker Erlenmeyer Graduated cylinder Buret Volumetric

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Electronic balances

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Small tissue culture laboratory

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Laminar flow hood

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Liquid dispensers

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Common light microscopes

Magnification: Resolution:

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CO2 incubator

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Sterilizing the material Vertical autoclave Horizontal autoclave

Sterilizing oven Bunsen burner

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Microplate readers - spectrometers

Colorimetry Fluorescence Fluorescence polarization Luminescence Microscopy

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Probability of contamination

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Reading material

Gallagher, S.R.; Wiley, E.A. “Current Protocols in Essential Laboratory Techniques” Volume/Weight Measurement Reagent Preparation Cell Culture Techniques Freshney, Y. “Culture of animal cells” Laboratory Design, Layout, and Equipment Equipment and Materials Aseptic Technique Safety, Bioethics, and Validation

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Planning the assay

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