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Engineering BioBrick vectors from BioBrick parts. Reshma P Shetty , Drew Endy and Thomas F Knight Jr. Outline. Background Synthetic Biology Terms Method Current Research Discussion. Synthetic Biology. What is Synthetic Biology? A Method Milestones Recombinant DNA - PowerPoint PPT Presentation
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Engineering BioBrick vectors from BioBrick parts
Reshma P Shetty, Drew Endy and Thomas F Knight Jr
Outline Background
Synthetic Biology Terms
Method Current Research Discussion
Synthetic Biology What is Synthetic Biology?
A Method Milestones
Recombinant DNA PCR(Polymerase Chain Reaction) Automated Sequencing Automated Construction Standards Abstractions
-Write
-------------------Read
Last 30 years
Synthetic Biology
Synthetic Biology (Cont.) Systems
Devices
Parts
DNA
Terms Vector Plasmid Polymerase Restriction enzyme Ligase
Method Goal
Standardize the BioBrick vectors’ assembly process Maintain
Cloning site Selection marker High copy orgin Terminators flanking the cloning site Verification primer annealing sites
Process Build new BioBrick vectors’ backbone Standard assembly for new BioBrick
Method(Cont.) Basic BioBrick
Method(Cont.) Build new BioBrick vectors’ backbone
Method(Cont.) Standard assembly for new BioBrick
Method(Cont.) Check
Cloning site Selection marker High copy orgin Terminators flanking the cloning site Verification primer annealing sites
Current Research More than 200 parts
Some Systems
Discussion Wetware
Biological film Digital counting machine
Interdisciplinary research
Computer aided design